Awh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Awh
Entrez Gene ID	38451
Full Name	Arrowhead
Synonyms	CG1072,Dmel\CG1072,awh,l(3)63Ea,l(3)63Ea/Awh,l(3)64Ea,l(3)SH9,l63Ea
General protein information	Preferred Names Arrowhead Names CG1072 gene product from transcript CG1072-RB Awh-PA Awh-PB Awh-PC Awh-PD CG1072-PA CG1072-PB CG1072-PC CG1072-PD arrowhead lethal(3)63Ea
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 63F4-63F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274450.1	NP_001261379.1	arrowhead, isoform C
NM_001274451.1	NP_001261380.1	arrowhead, isoform D
NM_168042.2	NP_728906.1	arrowhead, isoform B
NM_079183.3	NP_523907.2	arrowhead, isoform A

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Lhx8	NP_001012219.1
Macaca mulatta (Rhesus monkey)	LHX8	XP_001097664.2
Canis lupus familiaris (dog)	LHX8	XP_005622116.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006540	XP_316578.4
Bos taurus (cattle)	LHX8	XP_002686347.1
Gallus gallus (chicken)	LHX8	NP_001035556.1
Danio rerio (zebrafish)	lhx8a	NP_001003980.1
Homo sapiens (human)	LHX8	NP_001001933.1
Xenopus tropicalis (tropical clawed frog)	lhx8	NP_001015899.1
Pan troglodytes (chimpanzee)	LHX8	XP_524738.2
Mus musculus (house mouse)	Lhx8	NP_034843.2
Drosophila melanogaster (fruit fly)	Awh	NP_728906.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Awh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Awh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17423	NM_168042.2 Latest version!	Drosophila melanogaster arrowhead, transcript variant B (Awh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OFa17424	NM_079183.3 Latest version!	Drosophila melanogaster arrowhead, transcript variant A (Awh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OFa20552	NM_001274450.1 Latest version!	Drosophila melanogaster arrowhead, transcript variant C (Awh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OFa20553	NM_001274451.1 Latest version!	Drosophila melanogaster arrowhead, transcript variant D (Awh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Awh ( NM_168042.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728906.1 Drosophila melanogaster arrowhead, transcript variant B (Awh), mRNA.

Price & Availability↑

CloneID	OFa17423
Clone ID Related Accession (Same CDS sequence)	NM_168042.2
Accession Version	NM_168042.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	arrowhead, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168042.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGACTG AGCTGCGTTC CTGCGCAGCG TGCGGGGAAC CCATCTCGGA TCGGTTCTTC 
CTGGAGGTCG GCGGCTGCTC CTGGCACGCC CACTGCCTCC GCTGCTGCAT GTGCATGTGC 
CCCCTGGATC GCCAGCAGTC CTGCTTCATC CGGGAGAGGC AGGTGTACTG CAAGGCCGAC 
TACAGCAAAA ATTTCGGCGC CAAGTGCTCC AAGTGCTGTC GCGGCATCTC CGCCTCGGAT 
TGGGTGCGTC GGGCACGGGA GCTGGTCTTC CATCTAGCCT GCTTTGCCTG CGACCAGTGT 
GGACGCCAGT TGTCCACCGG CGAGCAGTTC GCCCTCATGG ATGACCGGGT GCTCTGCAAG 
GCTCATTATC TGGAGACGGT CGAGGGTGGC ACCACATCGA GCGACGAGGG CTGTGATGGC 
GATGGTTATC ACAAGAGCAA GACGAAACGG GTGCGCACCA CCTTCACCGA GGAGCAGTTG 
CAAGTGCTGC AGGCCAACTT CCAGATTGAC AGTAATCCGG ATGGCCAGGA TCTGGAGCGG 
ATCGCCTCGG TGACCGGTCT CAGCAAGCGT GTGACGCAAG TATGGTTCCA GAACTCGCGG 
GCGCGTCAGA AAAAACACAT ACATGCTGGT AAGAATAAAA TACGCGAACC GGAAGGAAGC 
TCATTTGCGC GTCATATTAA CCTGCAGTTA ACGTATTCAT TTCAGAATAA CGCACAGAAC 
CCAATGCATT TGAATGGCTC TAAAGCGGGT CTATACCCGA CTCATGAATC CTCTATGGAT 
GAATTGTCGC AGGACTCCAG TGTACACTGT ATGCCCAGCG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728906.1
CDS	537..1364
Translation	MKTELRSCAACGEPISDRFFLEVGGCSWHAHCLRCCMCMCPLDRQQSCFIRERQVYCKADYSKNFGAKCSKCCRGISASDWVRRARELVFHLACFACDQCGRQLSTGEQFALMDDRVLCKAHYLETVEGGTTSSDEGCDGDGYHKSKTKRVRTTFTEEQLQVLQANFQIDSNPDGQDLERIASVTGLSKRVTQVWFQNSRARQKKHIHAGKNKIREPEGSSFARHINLQLTYSFQNNAQNPMHLNGSKAGLYPTHESSMDELSQDSSVHCMPSEV

Target ORF information:

RefSeq Version	NM_168042.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster arrowhead, transcript variant B (Awh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168042.2

ORF Insert Sequence:

ATGAAGACTG AGCTGCGTTC CTGCGCAGCG TGCGGGGAAC CCATCTCGGA TCGGTTCTTC 
CTGGAGGTCG GCGGCTGCTC CTGGCACGCC CACTGCCTCC GCTGCTGCAT GTGCATGTGC 
CCCCTGGATC GCCAGCAGTC CTGCTTCATC CGGGAGAGGC AGGTGTACTG CAAGGCCGAC 
TACAGCAAAA ATTTCGGCGC CAAGTGCTCC AAGTGCTGTC GCGGCATCTC CGCCTCGGAT 
TGGGTGCGTC GGGCACGGGA GCTGGTCTTC CATCTAGCCT GCTTTGCCTG CGACCAGTGT 
GGACGCCAGT TGTCCACCGG CGAGCAGTTC GCCCTCATGG ATGACCGGGT GCTCTGCAAG 
GCTCATTATC TGGAGACGGT CGAGGGTGGC ACCACATCGA GCGACGAGGG CTGTGATGGC 
GATGGTTATC ACAAGAGCAA GACGAAACGG GTGCGCACCA CCTTCACCGA GGAGCAGTTG 
CAAGTGCTGC AGGCCAACTT CCAGATTGAC AGTAATCCGG ATGGCCAGGA TCTGGAGCGG 
ATCGCCTCGG TGACCGGTCT CAGCAAGCGT GTGACGCAAG TATGGTTCCA GAACTCGCGG 
GCGCGTCAGA AAAAACACAT ACATGCTGGT AAGAATAAAA TACGCGAACC GGAAGGAAGC 
TCATTTGCGC GTCATATTAA CCTGCAGTTA ACGTATTCAT TTCAGAATAA CGCACAGAAC 
CCAATGCATT TGAATGGCTC TAAAGCGGGT CTATACCCGA CTCATGAATC CTCTATGGAT 
GAATTGTCGC AGGACTCCAG TGTACACTGT ATGCCCAGCG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Awh ( NM_079183.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523907.2 Drosophila melanogaster arrowhead, transcript variant A (Awh), mRNA.

Price & Availability↑

CloneID	OFa17424
Clone ID Related Accession (Same CDS sequence)	NM_079183.3
Accession Version	NM_079183.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	arrowhead, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079183.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGACTG AGCTGCGTTC CTGCGCAGCG TGCGGGGAAC CCATCTCGGA TCGGTTCTTC 
CTGGAGGTCG GCGGCTGCTC CTGGCACGCC CACTGCCTCC GCTGCTGCAT GTGCATGTGC 
CCCCTGGATC GCCAGCAGTC CTGCTTCATC CGGGAGAGGC AGGTGTACTG CAAGGCCGAC 
TACAGCAAAA ATTTCGGCGC CAAGTGCTCC AAGTGCTGTC GCGGCATCTC CGCCTCGGAT 
TGGGTGCGTC GGGCACGGGA GCTGGTCTTC CATCTAGCCT GCTTTGCCTG CGACCAGTGT 
GGACGCCAGT TGTCCACCGG CGAGCAGTTC GCCCTCATGG ATGACCGGGT GCTCTGCAAG 
GCTCATTATC TGGAGACGGT CGAGGGTGGC ACCACATCGA GCGACGAGGG CTGTGATGGC 
GATGGTTATC ACAAGAGCAA GACGAAACGG GTGCGCACCA CCTTCACCGA GGAGCAGTTG 
CAAGTGCTGC AGGCCAACTT CCAGATTGAC AGTAATCCGG ATGGCCAGGA TCTGGAGCGG 
ATCGCCTCGG TGACCGGTCT CAGCAAGCGT GTGACGCAAG TATGGTTCCA GAACTCGCGG 
GCGCGTCAGA AAAAACACAT ACATGCTGGT AAGAATAAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523907.2
CDS	537..1181
Translation	MKTELRSCAACGEPISDRFFLEVGGCSWHAHCLRCCMCMCPLDRQQSCFIRERQVYCKADYSKNFGAKCSKCCRGISASDWVRRARELVFHLACFACDQCGRQLSTGEQFALMDDRVLCKAHYLETVEGGTTSSDEGCDGDGYHKSKTKRVRTTFTEEQLQVLQANFQIDSNPDGQDLERIASVTGLSKRVTQVWFQNSRARQKKHIHAGKNKK

Target ORF information:

RefSeq Version	NM_079183.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster arrowhead, transcript variant A (Awh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079183.3

ORF Insert Sequence:

ATGAAGACTG AGCTGCGTTC CTGCGCAGCG TGCGGGGAAC CCATCTCGGA TCGGTTCTTC 
CTGGAGGTCG GCGGCTGCTC CTGGCACGCC CACTGCCTCC GCTGCTGCAT GTGCATGTGC 
CCCCTGGATC GCCAGCAGTC CTGCTTCATC CGGGAGAGGC AGGTGTACTG CAAGGCCGAC 
TACAGCAAAA ATTTCGGCGC CAAGTGCTCC AAGTGCTGTC GCGGCATCTC CGCCTCGGAT 
TGGGTGCGTC GGGCACGGGA GCTGGTCTTC CATCTAGCCT GCTTTGCCTG CGACCAGTGT 
GGACGCCAGT TGTCCACCGG CGAGCAGTTC GCCCTCATGG ATGACCGGGT GCTCTGCAAG 
GCTCATTATC TGGAGACGGT CGAGGGTGGC ACCACATCGA GCGACGAGGG CTGTGATGGC 
GATGGTTATC ACAAGAGCAA GACGAAACGG GTGCGCACCA CCTTCACCGA GGAGCAGTTG 
CAAGTGCTGC AGGCCAACTT CCAGATTGAC AGTAATCCGG ATGGCCAGGA TCTGGAGCGG 
ATCGCCTCGG TGACCGGTCT CAGCAAGCGT GTGACGCAAG TATGGTTCCA GAACTCGCGG 
GCGCGTCAGA AAAAACACAT ACATGCTGGT AAGAATAAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Awh ( NM_001274450.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261379.1 Drosophila melanogaster arrowhead, transcript variant C (Awh), mRNA.

Price & Availability↑

CloneID	OFa20552
Clone ID Related Accession (Same CDS sequence)	NM_001274450.1
Accession Version	NM_001274450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	arrowhead, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGACTG AGCTGCGTTC CTGCGCAGCG TGCGGGGAAC CCATCTCGGA TCGGTTCTTC 
CTGGAGGTCG GCGGCTGCTC CTGGCACGCC CACTGCCTCC GCTGCTGCAT GTGCATGTGC 
CCCCTGGATC GCCAGCAGTC CTGCTTCATC CGGGAGAGGC AGGTGTACTG CAAGGCCGAC 
TACAGCAAAA ATTTCGGCGC CAAGTGCTCC AAGTGCTGTC GCGGCATCTC CGCCTCGGAT 
TGGGTGCGTC GGGCACGGGA GCTGGTCTTC CATCTAGCCT GCTTTGCCTG CGACCAGTGT 
GGACGCCAGT TGTCCACCGG CGAGCAGTTC GCCCTCATGG ATGACCGGGT GCTCTGCAAG 
GCTCATTATC TGGAGACGGT CGAGGGTGGC ACCACATCGA GCGACGAGGG CTGTGATGGC 
GATGGTTATC ACAAGAGCAA GACGAAACGG GTGCGCACCA CCTTCACCGA GGAGCAGTTG 
CAAGTGCTGC AGGCCAACTT CCAGATTGAC AGTAATCCGG ATGGCCAGGA TCTGGAGCGG 
ATCGCCTCGG TGACCGGTCT CAGCAAGCGT GTGACGCAAG TATGGTTCCA GAACTCGCGG 
GCGCGTCAGA AAAAACACAT ACATGCTGGT AAGAATAAAA TACGCGAACC GGAAGGAAGC 
TCATTTGCGC GTCATATTAA CCTGCAGTTA ACGTATTCAT TTCAGAATAA CGCACAGAAC 
CCAATGCATT TGAATGGCTC TAAAGCGGGT CTATACCCGA CTCATGAATC CTCTATGGAT 
GAATTGTCGC AGGACTCCAG TGTACACTGT ATGCCCAGCG AGGTGTGACT GGAGCAATCC 
TCGCCAGCTC GAGCACATCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261379.1
CDS	537..1400
Translation	MKTELRSCAACGEPISDRFFLEVGGCSWHAHCLRCCMCMCPLDRQQSCFIRERQVYCKADYSKNFGAKCSKCCRGISASDWVRRARELVFHLACFACDQCGRQLSTGEQFALMDDRVLCKAHYLETVEGGTTSSDEGCDGDGYHKSKTKRVRTTFTEEQLQVLQANFQIDSNPDGQDLERIASVTGLSKRVTQVWFQNSRARQKKHIHAGKNKIREPEGSSFARHINLQLTYSFQNNAQNPMHLNGSKAGLYPTHESSMDELSQDSSVHCMPSEV*LEQSSPARAHR

Target ORF information:

RefSeq Version	NM_001274450.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster arrowhead, transcript variant C (Awh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274450.1

ORF Insert Sequence:

ATGAAGACTG AGCTGCGTTC CTGCGCAGCG TGCGGGGAAC CCATCTCGGA TCGGTTCTTC 
CTGGAGGTCG GCGGCTGCTC CTGGCACGCC CACTGCCTCC GCTGCTGCAT GTGCATGTGC 
CCCCTGGATC GCCAGCAGTC CTGCTTCATC CGGGAGAGGC AGGTGTACTG CAAGGCCGAC 
TACAGCAAAA ATTTCGGCGC CAAGTGCTCC AAGTGCTGTC GCGGCATCTC CGCCTCGGAT 
TGGGTGCGTC GGGCACGGGA GCTGGTCTTC CATCTAGCCT GCTTTGCCTG CGACCAGTGT 
GGACGCCAGT TGTCCACCGG CGAGCAGTTC GCCCTCATGG ATGACCGGGT GCTCTGCAAG 
GCTCATTATC TGGAGACGGT CGAGGGTGGC ACCACATCGA GCGACGAGGG CTGTGATGGC 
GATGGTTATC ACAAGAGCAA GACGAAACGG GTGCGCACCA CCTTCACCGA GGAGCAGTTG 
CAAGTGCTGC AGGCCAACTT CCAGATTGAC AGTAATCCGG ATGGCCAGGA TCTGGAGCGG 
ATCGCCTCGG TGACCGGTCT CAGCAAGCGT GTGACGCAAG TATGGTTCCA GAACTCGCGG 
GCGCGTCAGA AAAAACACAT ACATGCTGGT AAGAATAAAA TACGCGAACC GGAAGGAAGC 
TCATTTGCGC GTCATATTAA CCTGCAGTTA ACGTATTCAT TTCAGAATAA CGCACAGAAC 
CCAATGCATT TGAATGGCTC TAAAGCGGGT CTATACCCGA CTCATGAATC CTCTATGGAT 
GAATTGTCGC AGGACTCCAG TGTACACTGT ATGCCCAGCG AGGTGTGACT GGAGCAATCC 
TCGCCAGCTC GAGCACATCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Awh ( NM_001274451.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261380.1 Drosophila melanogaster arrowhead, transcript variant D (Awh), mRNA.

Price & Availability↑

CloneID	OFa20553
Clone ID Related Accession (Same CDS sequence)	NM_001274451.1
Accession Version	NM_001274451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	arrowhead, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGACTG AGCTGCGTTC CTGCGCAGCG TGCGGGGAAC CCATCTCGGA TCGGTTCTTC 
CTGGAGGTCG GCGGCTGCTC CTGGCACGCC CACTGCCTCC GCTGCTGCAT GTGCATGTGC 
CCCCTGGATC GCCAGCAGTC CTGCTTCATC CGGGAGAGGC AGGTGTACTG CAAGGCCGAC 
TACAGCAAAA ATTTCGGCGC CAAGTGCTCC AAGTGCTGTC GCGGCATCTC CGCCTCGGAT 
TGGGTGCGTC GGGCACGGGA GCTGGTCTTC CATCTAGCCT GCTTTGCCTG CGACCAGTGT 
GGACGCCAGT TGTCCACCGG CGAGCAGTTC GCCCTCATGG ATGACCGGGT GCTCTGCAAG 
GCTCATTATC TGGAGACGGT CGAGGGTGGC ACCACATCGA GCGACGAGGG CTGTGATGGC 
GATGGTTATC ACAAGAGCAA GACGAAACGG GTGCGCACCA CCTTCACCGA GGAGCAGTTG 
CAAGTGCTGC AGGCCAACTT CCAGATTGAC AGTAATCCGG ATGGCCAGGA TCTGGAGCGG 
ATCGCCTCGG TGACCGGTCT CAGCAAGCGT GTGACGCAAG TATGGTTCCA GAACTCGCGG 
GCGCGTCAGA AAAAACACAT ACATGCTGTA CGCGAACCGG AAGGAAGCTC ATTTGCGCGT 
CATATTAACC TGCAGTTAAC GTATTCATTT CAGAATAACG CACAGAACCC AATGCATTTG 
AATGGCTCTA AAGCGGGTCT ATACCCGACT CATGAATCCT CTATGGATGA ATTGTCGCAG 
GACTCCAGTG TACACTGTAT GCCCAGCGAG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261380.1
CDS	537..1352
Translation	MKTELRSCAACGEPISDRFFLEVGGCSWHAHCLRCCMCMCPLDRQQSCFIRERQVYCKADYSKNFGAKCSKCCRGISASDWVRRARELVFHLACFACDQCGRQLSTGEQFALMDDRVLCKAHYLETVEGGTTSSDEGCDGDGYHKSKTKRVRTTFTEEQLQVLQANFQIDSNPDGQDLERIASVTGLSKRVTQVWFQNSRARQKKHIHAVREPEGSSFARHINLQLTYSFQNNAQNPMHLNGSKAGLYPTHESSMDELSQDSSVHCMPSEV

Target ORF information:

RefSeq Version	NM_001274451.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster arrowhead, transcript variant D (Awh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274451.1

ORF Insert Sequence:

ATGAAGACTG AGCTGCGTTC CTGCGCAGCG TGCGGGGAAC CCATCTCGGA TCGGTTCTTC 
CTGGAGGTCG GCGGCTGCTC CTGGCACGCC CACTGCCTCC GCTGCTGCAT GTGCATGTGC 
CCCCTGGATC GCCAGCAGTC CTGCTTCATC CGGGAGAGGC AGGTGTACTG CAAGGCCGAC 
TACAGCAAAA ATTTCGGCGC CAAGTGCTCC AAGTGCTGTC GCGGCATCTC CGCCTCGGAT 
TGGGTGCGTC GGGCACGGGA GCTGGTCTTC CATCTAGCCT GCTTTGCCTG CGACCAGTGT 
GGACGCCAGT TGTCCACCGG CGAGCAGTTC GCCCTCATGG ATGACCGGGT GCTCTGCAAG 
GCTCATTATC TGGAGACGGT CGAGGGTGGC ACCACATCGA GCGACGAGGG CTGTGATGGC 
GATGGTTATC ACAAGAGCAA GACGAAACGG GTGCGCACCA CCTTCACCGA GGAGCAGTTG 
CAAGTGCTGC AGGCCAACTT CCAGATTGAC AGTAATCCGG ATGGCCAGGA TCTGGAGCGG 
ATCGCCTCGG TGACCGGTCT CAGCAAGCGT GTGACGCAAG TATGGTTCCA GAACTCGCGG 
GCGCGTCAGA AAAAACACAT ACATGCTGTA CGCGAACCGG AAGGAAGCTC ATTTGCGCGT 
CATATTAACC TGCAGTTAAC GTATTCATTT CAGAATAACG CACAGAACCC AATGCATTTG 
AATGGCTCTA AAGCGGGTCT ATACCCGACT CATGAATCCT CTATGGATGA ATTGTCGCAG 
GACTCCAGTG TACACTGTAT GCCCAGCGAG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Awh cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

ORF Insert Sequence:

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