alpha-Spec cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	alpha-Spec
Entrez Gene ID	38231
Full Name	alpha Spectrin
Synonyms	3A9,CG1977,Dmel\CG1977,FBtr0072789,FCP-B,Spec,Spectrin,alfa-Spec,alpha,alpha spectrin,alpha-SPEC,alpha-Sp,alpha-Spc,alpha-Spe,alpha-Spect,alpha-Spectrin,alpha-spec,alpha-spectrin,alphaSp,alphaSpec,alphaSpectrin,anon-EST:fe2E2,dre3,klo,l(3)04276,l(3)62Bd,l(3)alpha-Spec,l(3)dre3,spectrin
General protein information	Preferred Names alpha Spectrin Names CG1977 gene product from transcript CG1977-RA CG1977-PA CG1977-PB CG1977-PC CG1977-PD aSpc 3A9 alpha spectrin alpha-SPECTRIN alpha-Spec-PA alpha-Spec-PB alpha-Spec-PC alpha-Spec-PD alpha-spectrin II alphaSpectrin anon-fast-evolving-2E2 klotzchen spectrin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 62B4-62B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274358.1	NP_001261287.1	alpha spectrin, isoform C
NM_001274359.1	NP_001261288.1	alpha spectrin, isoform D
NM_057391.4	NP_476739.1	alpha spectrin, isoform A
NM_001274357.1	NP_001261286.1	alpha spectrin, isoform B

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sptan1	NP_741984.2
Danio rerio (zebrafish)	spna2	NP_001091958.1
Caenorhabditis elegans (roundworm)	spc-1	NP_001257000.1
Xenopus tropicalis (tropical clawed frog)	sptan1	NP_001090674.1
Canis lupus familiaris (dog)	SPTAN1	XP_005625341.1
Bos taurus (cattle)	SPTAN1	NP_001107628.1
Mus musculus (house mouse)	Sptan1	NP_001171138.1
Gallus gallus (chicken)	SPTAN1	NP_001036003.1
Homo sapiens (human)	SPTAN1	NP_001123910.1
Macaca mulatta (Rhesus monkey)	SPTAN1	XP_002800011.1
Drosophila melanogaster (fruit fly)	alpha-Spec	NP_001261286.1
Pan troglodytes (chimpanzee)	SPTAN1	XP_003312345.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006686	XP_316724.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following alpha-Spec gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the alpha-Spec cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17228	NM_057391.4 Latest version!	Drosophila melanogaster alpha spectrin, transcript variant A (alpha-Spec), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OFa20437	NM_001274359.1 Latest version!	Drosophila melanogaster alpha spectrin, transcript variant D (alpha-Spec), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OFa20438	NM_001274358.1 Latest version!	Drosophila melanogaster alpha spectrin, transcript variant C (alpha-Spec), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OFa20439	NM_001274357.1 Latest version!	Drosophila melanogaster alpha spectrin, transcript variant B (alpha-Spec), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

alpha-Spec ( NM_057391.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476739.1 Drosophila melanogaster alpha spectrin, transcript variant A (alpha-Spec), mRNA.

Price & Availability↑

CloneID	OFa17228
Clone ID Related Accession (Same CDS sequence)	NM_057391.4
Accession Version	NM_057391.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	alpha spectrin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_057391.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAACT TTACACCCAA AGAGGTGAAG ATCCTCGAGA CAGTCGAGGA CATCCAGGAG 
CGACGTGAGC AGGTTCTGTC CCGCTACAAT GACTTCAAGA TCGAGACGCG CCAGAAGCGT 
GAAAAGCTCG AGGACTCGCG CCGCTTCCAG TACTTCAAGC GCGATGCCGA CGAGTTGGAG 
TCGTGGATCC ACGAGAAGCT GCAGGCAGCC AGCGAGGAGA GCTACCGCGA TCCGACCAAC 
CTGCAGGCCA AAATCCAGAA ACATCAGGCG TTCGAGGCGG AGGTGTCGGC GCACAGCAAC 
GCCATTGTCT CGCTGGACAA CACTGGCCAG GAGATGATCA ACCAGCAACA TTTTGCCTCC 
GAGTCGATTC AGGTCCGCCT CGACGAGCTG CACAAGCTGT GGGAGCTGCT TCTTAGCCGT 
CTGGCCGAGA AGGGTCTAAA GCTGCAGCAG GCTCTCGTCC TGGTGCAGTT CCTGCGCCAG 
TGCGAGGAGG TGATGTTCTG GATTAAGGAC AAGGAGACCT TTGTCACCGC CGACGAGTTC 
GGCCAAGACC TGGAACATGT AGAGGTTCTG CAGCGCAAGT TCGATGAGTT CCAGAAGGAC 
ATGGCCTCCC AGGAGTATCG CGTGACTGAG GTCAACCAGT TGGCCGATAA GTTGGTGCAA 
GATGGTCATC CCGAGCGCGA CACTATTACC AAGCGCAAGG AGGAGCTAAA CGAGGCTTGG 
CAGCGACTTA AGCAGTTAGC CATTGTGCGC CAAGAGAAGC TATTCGGTGC CCACGAGATC 
CAGCGTTTCA ATCGCGATGC CGACGAAACC GTCGCCTGGA TTGCCGAGAA GGATGTGGTC 
CTGTCGTCCG ATGATTATGG CCGCGATTTG GCCAGTGTTC AGGCGCTGCA ACGCAAACAC 
GAAGGAGTGG AGCGGGATTT GGCCGCCTTG GAGGATAAGG TTTCCACACT GGGAGCCGAG 
GCCCAGCGGC TGTGCTCCAT TCACGCCGAT CACAGTGACC AGATCCGTGA CAAACAGGCC 
GAGATTGCCA ACTACTGGCA GAGTCTCACC ACCAAGGCTC GCGAGCGCAA GCAGAAGCTA 
GATGAGTCCT ACTACCTGCA CCGTTTCCTC GCCGATTTCC GCGACCTCGT GTCCTGGATC 
AATGGCATGA AGGCCATCAT CTCGGCCGAT GAGCTGGCTA AGGATGTGGC TGGAGCAGAG 
GCTCTTCTGG AACGTCACCA GGAGCACAAG GGCGAGATCG ACGCTCGTGA GGATAGCTTC 
AAGCTGACCA CTGAATCCGG ACAGAAGCTG CTGGAGCGTG AGCATTACGC CGCCGCCGAA 
ATCCAGGAAA AGCTTGCCGC TCTGGAAAAC GACAAGAGTT CACTGCTCTC GCTGTGGGAA 
GATCGTCGCA TCCTGTACGA GCAGTGCATG GATCTGCAAC TGTTTTACCG CGATACGGAG 
CAGGCTGACA CTTGGATGGC CAAACAGGAG GCCTTCCTGG CCAACGAGGA CCTTGGCGAC 
TCCCTGGACT CTGTGGAGGC GCTAATTAAG AAGCACGAGG ACTTCGAGAA GAGTCTGGCT 
GCCCAGGAGG AGAAGATCAA GGCTTTAGAC ATCTTTGCCA CCAAATTAAT CGATGGTCAG 
CATTATGCCG CCGATGATGT GGCCCAGCGC CGTCAGATGC TCCTAGCCCG GCGGGCTGCT 
CTCCAAGAAA AATCGTCCAA GCGTCGCCAA TTGCTGGAGG ATTCGAACCG CTACCAGCAG 
TTCGAGCGCG ATTGCGACGA GACCAAGGGC TGGATTAGCG AGAAGCTGAA GTTTGCCACC 
GATGACAGCT ATTTGGATCC TACCAACCTT AACGGAAAGA TGCAGAAGCA CCAGAACTTT 
GAGCACGAGC TGAATGCCAA CAAGTCGCGC ATCGAGGACA TCACCAACGT GGGCACCGAG 
CTGATCGAGA AGCAGCACTA TGCTGCCGAC CAGATCAACA CCCGCATGCA GGAGATCGTC 
GTCCTGTGGG AGACCCTCGT CCAGGCTTCC GATAAAAAGG GAACCAAACT CAACGAGGCA 
TGCCAACAGC AGCAGTTCAA CCGCACCATC GAGGACATTG AGCTGTGGCT TAGCGAGATC 
GAGGGTCAAT TGTTGTCGGA GGATCACGGC AAAGACCTGA CCTCCGTTCA GAATTTGCAG 
AAGAAGCACG CCCTGCTGGA GGCCGATGTG ATGGCTCACC AGGATCGCAT TGAAAGCATC 
AAAGTGGCCG CCAACAAGTT CATCGAATCC GGTCACTTTG ACGCGGACAA CATTCGCAAC 
AAGGAGGGCA ATCTGTCGGC TCGCTATGCC GCCCTAGCTG CACCCATGGG CGAGAGGAAA 
CAACACCTTC TTGACTCCCT TCAGGTGCAG CAGCTCTTCC GCGACTTGGA GGACGAGGCT 
GCCTGGATCC GTGAGAAGGA ACCCATTGCC GCATCCACGA ACCGCGGTCG TGACTTGATT 
GGTGTGCAAA ACCTGATCAA GAAACACCAG GCTGTGCTGG CCGAGATCAA CAACCATGAG 
GCCAGGCTGC TGAATGTGAT CAGTTCGGGC GAGAACATGC TGAAGGACCA GCCCTTCGCC 
AGCGACGATA TCCGTCAGCG TTTGGAGGCG CTCCAAGAGC AGTGGAACAC GCTGAAGGAG 
AAGTCCAGCC AGCGAAAGCA GGATCTGGAT GACTCTCTCC AGGCACACCA GTACTTTGCC 
GACGCCAACG AAGCTGAGTC GTGGATGCGC GAAAAGGAAC CGATTGCAAC TGGCAGTGAT 
TATGGTAAGG ACGAGGATTC CTCGGAGGCT TTGCTCAAGA AGCACGAGGC TCTGGTCTCC 
GATCTAGAGG CTTTTGGCAA CACCATTCAG GCGCTGCAGG AGCAGGCCAA GAACTGTCGC 
CAGCAGGAGA CCCCTGTTGT GGATATCACT GGCAAGGAGT GCGTGGTTGC CCTCTACGAC 
TACACGGAGA AATCCCCTCG TGAGGTCTCG ATGAAGAAGG GCGATGTTCT GACCCTGCTG 
AACTCGAACA ACAAAGACTG GTGGAAGGTG GAGGTCAACG ACCGCCAGGG CTTCGTACCG 
GCCGCATACA TCAAGAAGAT TGACGCCGGC TTGAGCGCCT CCCAGCAGAA CCTAGTGGAC 
AATCATTCGA TTGCCAAGCG CCAGAACCAG ATCAACTCGC AGTACGACAA CTTGCTGGCC 
CTCGCCCGCG AGCGCCAGAA CAAGCTAAAC GAGACTGTGA AGGCGTACGT TCTTGTTCGT 
GAGGCTGCCG ACTTGGCCCA GTGGATCCGG GACAAGGAGA ACCACGCTCA GATCGCTGAT 
GTTGTGGGAG AGGATCTGGA AGAGGTGGAG GTGCTCCAAA AGAAGTTCGA CGACTTCAAC 
GACGACCTGA AGGCCAACGA AGTGCGTCTG GCAAACATGA ACGAGATTGC CGTGCAGCTG 
ACTTCCTTGG GTCAGACCGA GGCCGCCCTA AAGATCCAAA CCCAGATGCA AGACCTGAAC 
GAGAAGTGGA ACAACCTGCA GACGCTGACC GCTGAGAAGG CCAGCCAACT GGGATCCGCC 
CATGAGGTGC AACGCTTCCA TCGTGACATT GACGAGACCA AGGATTGGAT CGCTGAGAAG 
GCCAATGCCC TGAACAACGA CGACCTGGGC AAGGATCTGC GCAGTGTCCA GACTCTGCAG 
CGCAAGCACG AGGGCGTGGA ACGCGACTTG GCCGCATTGC GGGACAAGAT CCGCCAGCTG 
GACGAAACCG CCAACAGGCT GATGCAGTCG CACCCGGACA CCGCCGAGCA GACCTACGCC 
AAGCAGAAGG AGATCAACGA GATGTGGGAC CAGATCATCA CCAAGTCCAC CGCCCGCAAG 
GAGAAGCTGC TGGACTCGTA CGACCTGCAG CGTTTCCTCA GCGACTACCG CGATCTGCTG 
GCCTGGATCA ACTCGATGAT GAGCCTGGTC ACGTCCGACG AGCTGGCCAA CGATGTAACA 
GGAGCCGAGG CTCTGATCGA GCGTCATCAG GAACACCGAA CGGAGATCGA TGCGCGCGCC 
GGAACTTTCG GGGCTTTCGA GCAGTTCGGA AACGAGCTGC TGCAGGCCAA CCACTATGCG 
TCGCCTGAAA TCAAGGAGAA AATCGAGGAT CTGGCCAAGG CCCGCGAGGA CTTGGAGAAG 
GCGTGGACAG AGCGCCGCCT GCAGCTGGAG CAGAACTTGG ATCTGCAGCT CTACATGCGT 
GACTGCGAGT TGGCCGAGTC CTGGATGTCA GCCCGCGAGG CCTTCCTAAA TGCCGATGAT 
GATGCCAATG CCGGCGGAAA TGTGGAGGCA CTGATCAAGA AGCACGAGGA CTTCGACAAG 
GCCATTAACG GCCACGAACA GAAGATTGCT GCTCTGCAGA CGGTGGCGGA TCAGTTGATC 
GCCCAGAACC ACTATGCCTC CAATTTGGTG GACGAGAAGC GCAAGCAAGT GCTGGAGCGC 
TGGCGCCATC TCAAGGAGGG TCTGATCGAG AAGCGATCCC GTTTGGGTGA CGAGCAGACG 
CTGCAGCAGT TCTCGCGCGA TGCCGATGAG ATTGAGAACT GGATTGCAGA GAAGTTGCAG 
CTGGCCACCG AAGAGAGCTA CAAGGACCCG GCTAACATTC AGTCGAAGCA CCAGAAGCAC 
CAGGCCTTCG AGGCGGAGCT GGCGGCTAAC GCCGATCGCA TCCAGAGCGT GCTAGCCATG 
GGCGGAAACC TCATCGATAA GAAGCAGTGC AGCGGGTCGG AGGACGCGGT GCAGAAGCGG 
CTGACGCAGA TCGCCGATCA GTGGGAGTAC CTGACCCATA AGACTACCGA GAAGTCGCTG 
AAGCTAAAGG AGGCGAATAA GCAGCGCACC TATATCGCTG CTGTCAAGGA TTTGGACTTC 
TGGTTGGGCG AGGTCGAGAG TCTTTTGACC ACTGAGGATT CTGGTAAGGA TTTGGCCTCT 
GTCCAAAACC TCATGAAGAA GCATCAGTTG GTCGAGGCGG ATATCGTGGC GCACGAAGAC 
CGCATCAAGG ACATGAACAA CCAGGCCGAT TCCTTGGTGG AGAGCGGCCA GTTCGATACT 
GCCGGCATCC AGGAGAAGCG TCAAAGCATC AATGAGCGCT ACGAGCGCAT CTGCAACCTG 
GCGGCCCATC GCCAAGCCCG TCTCAACGAG GCTCTGACCC TGCACCAATT CTTCCGCGAC 
ATCGCCGACG AGGAGAGCTG GATCAAGGAG AAGAAGCTAC TTGTCGGCTC CGACGACTAT 
GGTCGCGATC TGACCGGCGT CCAGAACCTC AAGAAGAAGC ACAAGCGCCT AGAAGCCGAA 
TTGGGCTCTC ACGAGCCGGC CATTCAAGCT GTGCAGGAGG CTGGCGAGAA GCTCATGGAC 
GTGTCCAACC TCGGTGTCCC CGAAATTGAG CAGCGCCTGA AGGCTCTGAA CCAGGCCTGG 
GCCGAGCTAA AGAACCTGGC TGCCACGCGA GGTCAGAAGT TGGATGAGTC GCTGACCTAT 
CAGCAATTCC TCGCTCAGGT GGAGGAGGAG GAGGCCTGGA TCACCGAGAA GCAACAGCTG 
CTCTCTGTGG AGGACTACGG CGACTCGATG GCCGCCGTAC AAGGACTGCT GAAAAAGCAC 
GACGCATTCG AAACGGACTT CACTGCCCAC AAGGACCGCT GTTCTTTGAT CTGCGACCAG 
GGCAGTGAGT TGGTGGAGGC CAAGAACCAC CACGGAGAGT CCATTGCCCA ACGCTGCCAG 
CAGCTGCGCC TGAAACTGGA CAACCTGTCC GCCCTGGCTG CCCGCCGCAA GGGTGCCCTG 
CTGGACAACT CGGCCTATCT GCAGTTCATG TGGAAGGCCG ATGTGGTCGA GAGCTGGATC 
GACGACAAGG AGAACTATGT GCGCTCCGAT GAGTTCGGAC GCGACCTGTC CACCGTGCAA 
ACGCTGTTGA CCAAGCAAGA GACATTCGAT GCAGGTCTCA ATGCCTTCGA GCAGGAGGGC 
ATACACAACA TCACGGCACT TAAGGACCAA CTGATAAATG CCAGTCACGC CCAGTCGCCG 
GCAATCCTAA AGCGTCACGG AGACGTGATT GCCAGGTGGC AGAAGTTGCG CGACGCCTCC 
AACACGCGCA AGGACCGACT GCTGGCCATG CAGGAGCAGT TCCGCCAGAT CGAGGAACTC 
TACTTGACAT TTGCCAAGAA AGCTTCGGCC TTCAATTCTT GGTTCGAAAA TGCCGAGGAG 
GATCTCACAG ATCCTGTTCG CTGCAATTCG ATCGAAGAAA TCCGCGCCCT GCGCGACGCC 
CATGCCCAAT TCCAGGCGTC CCTTTCGTCG GCCGAAGCTG ACTTCAAGGC ATTGGCAGCA 
CTCGACCAGA AGATCAAGAG CTTTAATGTT GGACCCAACC CGTACACGTG GTTCACCATG 
GAAGCTCTTG AGGAGACCTG GCGTAATCTG CAAAAGATCA TAGAGGAACG CGATGGGGAA 
CTTGCCAAGG AGGCCAAGCG GCAGGAGGAG AACGACAAGC TGCGCAAGGA GTTTGCCAAG 
CACGCCAATC TTTTCCACCA GTGGCTAACA GAGACAAGAA CGTCCATGAT GGAAGGCTCC 
GGTTCCCTGG AACAGCAACT GGAGGCGCTT CGTGTCAAGG CCACCGAGGT GCGCGCACGT 
CGCGTTGACC TCAAGAAGAT CGAGGAGCTG GGAGCCTTGC TGGAGGAGCA CCTTATTCTG 
GACAACCGCT ACACGGAACA CTCGACGGTG GGCCTGGCCC AGCAGTGGGA TCAGCTTGAC 
CAGCTGTCTA TGCGCATGCA GCACAACTTA GAGCAACAGA TCCAGGCACG CAACCACTCG 
GGCGTCTCCG AGGACTCACT CAAGGAGTTC TCGATGATGT TCAAGCACTT CGATAAGGAC 
AAGAGCGGCA AGCTCAACCA CCAGGAATTC AAGTCCTGCC TGCGCGCCCT CGGCTACGAC 
CTGCCCATGG TGGAGGAGGG ACAGCCCGAC CCGGAGTTCG AAGCAATTCT CGATGTTGTC 
GACCCCAACC GCGATGGCTA CGTCTCTCTG CAGGAATACA TCGCGTTCAT GATCTCCAAG 
GAGACGGAGA ACGTTCAATC CTACGAGGAG ATCGAGAATG CCTTCCGCGC CATCACCGCT 
GCCGACCGCC CCTATGTAAC TAAGGAGGAG CTCTACTGCA ACCTCACCAA GGACATGGCT 
GACTACTGCG TGCAGCGCAT GAAACCCTTC TCGGAACCGC GCTCCGGCCA GCCCATCAAG 
GATGCCCTGG ACTACATAGA CTTCACGCGA ACGCTGTTCC AGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476739.1
CDS	277..7524
Translation	MENFTPKEVKILETVEDIQERREQVLSRYNDFKIETRQKREKLEDSRRFQYFKRDADELESWIHEKLQAASEESYRDPTNLQAKIQKHQAFEAEVSAHSNAIVSLDNTGQEMINQQHFASESIQVRLDELHKLWELLLSRLAEKGLKLQQALVLVQFLRQCEEVMFWIKDKETFVTADEFGQDLEHVEVLQRKFDEFQKDMASQEYRVTEVNQLADKLVQDGHPERDTITKRKEELNEAWQRLKQLAIVRQEKLFGAHEIQRFNRDADETVAWIAEKDVVLSSDDYGRDLASVQALQRKHEGVERDLAALEDKVSTLGAEAQRLCSIHADHSDQIRDKQAEIANYWQSLTTKARERKQKLDESYYLHRFLADFRDLVSWINGMKAIISADELAKDVAGAEALLERHQEHKGEIDAREDSFKLTTESGQKLLEREHYAAAEIQEKLAALENDKSSLLSLWEDRRILYEQCMDLQLFYRDTEQADTWMAKQEAFLANEDLGDSLDSVEALIKKHEDFEKSLAAQEEKIKALDIFATKLIDGQHYAADDVAQRRQMLLARRAALQEKSSKRRQLLEDSNRYQQFERDCDETKGWISEKLKFATDDSYLDPTNLNGKMQKHQNFEHELNANKSRIEDITNVGTELIEKQHYAADQINTRMQEIVVLWETLVQASDKKGTKLNEACQQQQFNRTIEDIELWLSEIEGQLLSEDHGKDLTSVQNLQKKHALLEADVMAHQDRIESIKVAANKFIESGHFDADNIRNKEGNLSARYAALAAPMGERKQHLLDSLQVQQLFRDLEDEAAWIREKEPIAASTNRGRDLIGVQNLIKKHQAVLAEINNHEARLLNVISSGENMLKDQPFASDDIRQRLEALQEQWNTLKEKSSQRKQDLDDSLQAHQYFADANEAESWMREKEPIATGSDYGKDEDSSEALLKKHEALVSDLEAFGNTIQALQEQAKNCRQQETPVVDITGKECVVALYDYTEKSPREVSMKKGDVLTLLNSNNKDWWKVEVNDRQGFVPAAYIKKIDAGLSASQQNLVDNHSIAKRQNQINSQYDNLLALARERQNKLNETVKAYVLVREAADLAQWIRDKENHAQIADVVGEDLEEVEVLQKKFDDFNDDLKANEVRLANMNEIAVQLTSLGQTEAALKIQTQMQDLNEKWNNLQTLTAEKASQLGSAHEVQRFHRDIDETKDWIAEKANALNNDDLGKDLRSVQTLQRKHEGVERDLAALRDKIRQLDETANRLMQSHPDTAEQTYAKQKEINEMWDQIITKSTARKEKLLDSYDLQRFLSDYRDLLAWINSMMSLVTSDELANDVTGAEALIERHQEHRTEIDARAGTFGAFEQFGNELLQANHYASPEIKEKIEDLAKAREDLEKAWTERRLQLEQNLDLQLYMRDCELAESWMSAREAFLNADDDANAGGNVEALIKKHEDFDKAINGHEQKIAALQTVADQLIAQNHYASNLVDEKRKQVLERWRHLKEGLIEKRSRLGDEQTLQQFSRDADEIENWIAEKLQLATEESYKDPANIQSKHQKHQAFEAELAANADRIQSVLAMGGNLIDKKQCSGSEDAVQKRLTQIADQWEYLTHKTTEKSLKLKEANKQRTYIAAVKDLDFWLGEVESLLTTEDSGKDLASVQNLMKKHQLVEADIVAHEDRIKDMNNQADSLVESGQFDTAGIQEKRQSINERYERICNLAAHRQARLNEALTLHQFFRDIADEESWIKEKKLLVGSDDYGRDLTGVQNLKKKHKRLEAELGSHEPAIQAVQEAGEKLMDVSNLGVPEIEQRLKALNQAWAELKNLAATRGQKLDESLTYQQFLAQVEEEEAWITEKQQLLSVEDYGDSMAAVQGLLKKHDAFETDFTAHKDRCSLICDQGSELVEAKNHHGESIAQRCQQLRLKLDNLSALAARRKGALLDNSAYLQFMWKADVVESWIDDKENYVRSDEFGRDLSTVQTLLTKQETFDAGLNAFEQEGIHNITALKDQLINASHAQSPAILKRHGDVIARWQKLRDASNTRKDRLLAMQEQFRQIEELYLTFAKKASAFNSWFENAEEDLTDPVRCNSIEEIRALRDAHAQFQASLSSAEADFKALAALDQKIKSFNVGPNPYTWFTMEALEETWRNLQKIIEERDGELAKEAKRQEENDKLRKEFAKHANLFHQWLTETRTSMMEGSGSLEQQLEALRVKATEVRARRVDLKKIEELGALLEEHLILDNRYTEHSTVGLAQQWDQLDQLSMRMQHNLEQQIQARNHSGVSEDSLKEFSMMFKHFDKDKSGKLNHQEFKSCLRALGYDLPMVEEGQPDPEFEAILDVVDPNRDGYVSLQEYIAFMISKETENVQSYEEIENAFRAITAADRPYVTKEELYCNLTKDMADYCVQRMKPFSEPRSGQPIKDALDYIDFTRTLFQN

Target ORF information:

RefSeq Version	NM_057391.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alpha spectrin, transcript variant A (alpha-Spec), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057391.4

ORF Insert Sequence:

ATGGAGAACT TTACACCCAA AGAGGTGAAG ATCCTCGAGA CAGTCGAGGA CATCCAGGAG 
CGACGTGAGC AGGTTCTGTC CCGCTACAAT GACTTCAAGA TCGAGACGCG CCAGAAGCGT 
GAAAAGCTCG AGGACTCGCG CCGCTTCCAG TACTTCAAGC GCGATGCCGA CGAGTTGGAG 
TCGTGGATCC ACGAGAAGCT GCAGGCAGCC AGCGAGGAGA GCTACCGCGA TCCGACCAAC 
CTGCAGGCCA AAATCCAGAA ACATCAGGCG TTCGAGGCGG AGGTGTCGGC GCACAGCAAC 
GCCATTGTCT CGCTGGACAA CACTGGCCAG GAGATGATCA ACCAGCAACA TTTTGCCTCC 
GAGTCGATTC AGGTCCGCCT CGACGAGCTG CACAAGCTGT GGGAGCTGCT TCTTAGCCGT 
CTGGCCGAGA AGGGTCTAAA GCTGCAGCAG GCTCTCGTCC TGGTGCAGTT CCTGCGCCAG 
TGCGAGGAGG TGATGTTCTG GATTAAGGAC AAGGAGACCT TTGTCACCGC CGACGAGTTC 
GGCCAAGACC TGGAACATGT AGAGGTTCTG CAGCGCAAGT TCGATGAGTT CCAGAAGGAC 
ATGGCCTCCC AGGAGTATCG CGTGACTGAG GTCAACCAGT TGGCCGATAA GTTGGTGCAA 
GATGGTCATC CCGAGCGCGA CACTATTACC AAGCGCAAGG AGGAGCTAAA CGAGGCTTGG 
CAGCGACTTA AGCAGTTAGC CATTGTGCGC CAAGAGAAGC TATTCGGTGC CCACGAGATC 
CAGCGTTTCA ATCGCGATGC CGACGAAACC GTCGCCTGGA TTGCCGAGAA GGATGTGGTC 
CTGTCGTCCG ATGATTATGG CCGCGATTTG GCCAGTGTTC AGGCGCTGCA ACGCAAACAC 
GAAGGAGTGG AGCGGGATTT GGCCGCCTTG GAGGATAAGG TTTCCACACT GGGAGCCGAG 
GCCCAGCGGC TGTGCTCCAT TCACGCCGAT CACAGTGACC AGATCCGTGA CAAACAGGCC 
GAGATTGCCA ACTACTGGCA GAGTCTCACC ACCAAGGCTC GCGAGCGCAA GCAGAAGCTA 
GATGAGTCCT ACTACCTGCA CCGTTTCCTC GCCGATTTCC GCGACCTCGT GTCCTGGATC 
AATGGCATGA AGGCCATCAT CTCGGCCGAT GAGCTGGCTA AGGATGTGGC TGGAGCAGAG 
GCTCTTCTGG AACGTCACCA GGAGCACAAG GGCGAGATCG ACGCTCGTGA GGATAGCTTC 
AAGCTGACCA CTGAATCCGG ACAGAAGCTG CTGGAGCGTG AGCATTACGC CGCCGCCGAA 
ATCCAGGAAA AGCTTGCCGC TCTGGAAAAC GACAAGAGTT CACTGCTCTC GCTGTGGGAA 
GATCGTCGCA TCCTGTACGA GCAGTGCATG GATCTGCAAC TGTTTTACCG CGATACGGAG 
CAGGCTGACA CTTGGATGGC CAAACAGGAG GCCTTCCTGG CCAACGAGGA CCTTGGCGAC 
TCCCTGGACT CTGTGGAGGC GCTAATTAAG AAGCACGAGG ACTTCGAGAA GAGTCTGGCT 
GCCCAGGAGG AGAAGATCAA GGCTTTAGAC ATCTTTGCCA CCAAATTAAT CGATGGTCAG 
CATTATGCCG CCGATGATGT GGCCCAGCGC CGTCAGATGC TCCTAGCCCG GCGGGCTGCT 
CTCCAAGAAA AATCGTCCAA GCGTCGCCAA TTGCTGGAGG ATTCGAACCG CTACCAGCAG 
TTCGAGCGCG ATTGCGACGA GACCAAGGGC TGGATTAGCG AGAAGCTGAA GTTTGCCACC 
GATGACAGCT ATTTGGATCC TACCAACCTT AACGGAAAGA TGCAGAAGCA CCAGAACTTT 
GAGCACGAGC TGAATGCCAA CAAGTCGCGC ATCGAGGACA TCACCAACGT GGGCACCGAG 
CTGATCGAGA AGCAGCACTA TGCTGCCGAC CAGATCAACA CCCGCATGCA GGAGATCGTC 
GTCCTGTGGG AGACCCTCGT CCAGGCTTCC GATAAAAAGG GAACCAAACT CAACGAGGCA 
TGCCAACAGC AGCAGTTCAA CCGCACCATC GAGGACATTG AGCTGTGGCT TAGCGAGATC 
GAGGGTCAAT TGTTGTCGGA GGATCACGGC AAAGACCTGA CCTCCGTTCA GAATTTGCAG 
AAGAAGCACG CCCTGCTGGA GGCCGATGTG ATGGCTCACC AGGATCGCAT TGAAAGCATC 
AAAGTGGCCG CCAACAAGTT CATCGAATCC GGTCACTTTG ACGCGGACAA CATTCGCAAC 
AAGGAGGGCA ATCTGTCGGC TCGCTATGCC GCCCTAGCTG CACCCATGGG CGAGAGGAAA 
CAACACCTTC TTGACTCCCT TCAGGTGCAG CAGCTCTTCC GCGACTTGGA GGACGAGGCT 
GCCTGGATCC GTGAGAAGGA ACCCATTGCC GCATCCACGA ACCGCGGTCG TGACTTGATT 
GGTGTGCAAA ACCTGATCAA GAAACACCAG GCTGTGCTGG CCGAGATCAA CAACCATGAG 
GCCAGGCTGC TGAATGTGAT CAGTTCGGGC GAGAACATGC TGAAGGACCA GCCCTTCGCC 
AGCGACGATA TCCGTCAGCG TTTGGAGGCG CTCCAAGAGC AGTGGAACAC GCTGAAGGAG 
AAGTCCAGCC AGCGAAAGCA GGATCTGGAT GACTCTCTCC AGGCACACCA GTACTTTGCC 
GACGCCAACG AAGCTGAGTC GTGGATGCGC GAAAAGGAAC CGATTGCAAC TGGCAGTGAT 
TATGGTAAGG ACGAGGATTC CTCGGAGGCT TTGCTCAAGA AGCACGAGGC TCTGGTCTCC 
GATCTAGAGG CTTTTGGCAA CACCATTCAG GCGCTGCAGG AGCAGGCCAA GAACTGTCGC 
CAGCAGGAGA CCCCTGTTGT GGATATCACT GGCAAGGAGT GCGTGGTTGC CCTCTACGAC 
TACACGGAGA AATCCCCTCG TGAGGTCTCG ATGAAGAAGG GCGATGTTCT GACCCTGCTG 
AACTCGAACA ACAAAGACTG GTGGAAGGTG GAGGTCAACG ACCGCCAGGG CTTCGTACCG 
GCCGCATACA TCAAGAAGAT TGACGCCGGC TTGAGCGCCT CCCAGCAGAA CCTAGTGGAC 
AATCATTCGA TTGCCAAGCG CCAGAACCAG ATCAACTCGC AGTACGACAA CTTGCTGGCC 
CTCGCCCGCG AGCGCCAGAA CAAGCTAAAC GAGACTGTGA AGGCGTACGT TCTTGTTCGT 
GAGGCTGCCG ACTTGGCCCA GTGGATCCGG GACAAGGAGA ACCACGCTCA GATCGCTGAT 
GTTGTGGGAG AGGATCTGGA AGAGGTGGAG GTGCTCCAAA AGAAGTTCGA CGACTTCAAC 
GACGACCTGA AGGCCAACGA AGTGCGTCTG GCAAACATGA ACGAGATTGC CGTGCAGCTG 
ACTTCCTTGG GTCAGACCGA GGCCGCCCTA AAGATCCAAA CCCAGATGCA AGACCTGAAC 
GAGAAGTGGA ACAACCTGCA GACGCTGACC GCTGAGAAGG CCAGCCAACT GGGATCCGCC 
CATGAGGTGC AACGCTTCCA TCGTGACATT GACGAGACCA AGGATTGGAT CGCTGAGAAG 
GCCAATGCCC TGAACAACGA CGACCTGGGC AAGGATCTGC GCAGTGTCCA GACTCTGCAG 
CGCAAGCACG AGGGCGTGGA ACGCGACTTG GCCGCATTGC GGGACAAGAT CCGCCAGCTG 
GACGAAACCG CCAACAGGCT GATGCAGTCG CACCCGGACA CCGCCGAGCA GACCTACGCC 
AAGCAGAAGG AGATCAACGA GATGTGGGAC CAGATCATCA CCAAGTCCAC CGCCCGCAAG 
GAGAAGCTGC TGGACTCGTA CGACCTGCAG CGTTTCCTCA GCGACTACCG CGATCTGCTG 
GCCTGGATCA ACTCGATGAT GAGCCTGGTC ACGTCCGACG AGCTGGCCAA CGATGTAACA 
GGAGCCGAGG CTCTGATCGA GCGTCATCAG GAACACCGAA CGGAGATCGA TGCGCGCGCC 
GGAACTTTCG GGGCTTTCGA GCAGTTCGGA AACGAGCTGC TGCAGGCCAA CCACTATGCG 
TCGCCTGAAA TCAAGGAGAA AATCGAGGAT CTGGCCAAGG CCCGCGAGGA CTTGGAGAAG 
GCGTGGACAG AGCGCCGCCT GCAGCTGGAG CAGAACTTGG ATCTGCAGCT CTACATGCGT 
GACTGCGAGT TGGCCGAGTC CTGGATGTCA GCCCGCGAGG CCTTCCTAAA TGCCGATGAT 
GATGCCAATG CCGGCGGAAA TGTGGAGGCA CTGATCAAGA AGCACGAGGA CTTCGACAAG 
GCCATTAACG GCCACGAACA GAAGATTGCT GCTCTGCAGA CGGTGGCGGA TCAGTTGATC 
GCCCAGAACC ACTATGCCTC CAATTTGGTG GACGAGAAGC GCAAGCAAGT GCTGGAGCGC 
TGGCGCCATC TCAAGGAGGG TCTGATCGAG AAGCGATCCC GTTTGGGTGA CGAGCAGACG 
CTGCAGCAGT TCTCGCGCGA TGCCGATGAG ATTGAGAACT GGATTGCAGA GAAGTTGCAG 
CTGGCCACCG AAGAGAGCTA CAAGGACCCG GCTAACATTC AGTCGAAGCA CCAGAAGCAC 
CAGGCCTTCG AGGCGGAGCT GGCGGCTAAC GCCGATCGCA TCCAGAGCGT GCTAGCCATG 
GGCGGAAACC TCATCGATAA GAAGCAGTGC AGCGGGTCGG AGGACGCGGT GCAGAAGCGG 
CTGACGCAGA TCGCCGATCA GTGGGAGTAC CTGACCCATA AGACTACCGA GAAGTCGCTG 
AAGCTAAAGG AGGCGAATAA GCAGCGCACC TATATCGCTG CTGTCAAGGA TTTGGACTTC 
TGGTTGGGCG AGGTCGAGAG TCTTTTGACC ACTGAGGATT CTGGTAAGGA TTTGGCCTCT 
GTCCAAAACC TCATGAAGAA GCATCAGTTG GTCGAGGCGG ATATCGTGGC GCACGAAGAC 
CGCATCAAGG ACATGAACAA CCAGGCCGAT TCCTTGGTGG AGAGCGGCCA GTTCGATACT 
GCCGGCATCC AGGAGAAGCG TCAAAGCATC AATGAGCGCT ACGAGCGCAT CTGCAACCTG 
GCGGCCCATC GCCAAGCCCG TCTCAACGAG GCTCTGACCC TGCACCAATT CTTCCGCGAC 
ATCGCCGACG AGGAGAGCTG GATCAAGGAG AAGAAGCTAC TTGTCGGCTC CGACGACTAT 
GGTCGCGATC TGACCGGCGT CCAGAACCTC AAGAAGAAGC ACAAGCGCCT AGAAGCCGAA 
TTGGGCTCTC ACGAGCCGGC CATTCAAGCT GTGCAGGAGG CTGGCGAGAA GCTCATGGAC 
GTGTCCAACC TCGGTGTCCC CGAAATTGAG CAGCGCCTGA AGGCTCTGAA CCAGGCCTGG 
GCCGAGCTAA AGAACCTGGC TGCCACGCGA GGTCAGAAGT TGGATGAGTC GCTGACCTAT 
CAGCAATTCC TCGCTCAGGT GGAGGAGGAG GAGGCCTGGA TCACCGAGAA GCAACAGCTG 
CTCTCTGTGG AGGACTACGG CGACTCGATG GCCGCCGTAC AAGGACTGCT GAAAAAGCAC 
GACGCATTCG AAACGGACTT CACTGCCCAC AAGGACCGCT GTTCTTTGAT CTGCGACCAG 
GGCAGTGAGT TGGTGGAGGC CAAGAACCAC CACGGAGAGT CCATTGCCCA ACGCTGCCAG 
CAGCTGCGCC TGAAACTGGA CAACCTGTCC GCCCTGGCTG CCCGCCGCAA GGGTGCCCTG 
CTGGACAACT CGGCCTATCT GCAGTTCATG TGGAAGGCCG ATGTGGTCGA GAGCTGGATC 
GACGACAAGG AGAACTATGT GCGCTCCGAT GAGTTCGGAC GCGACCTGTC CACCGTGCAA 
ACGCTGTTGA CCAAGCAAGA GACATTCGAT GCAGGTCTCA ATGCCTTCGA GCAGGAGGGC 
ATACACAACA TCACGGCACT TAAGGACCAA CTGATAAATG CCAGTCACGC CCAGTCGCCG 
GCAATCCTAA AGCGTCACGG AGACGTGATT GCCAGGTGGC AGAAGTTGCG CGACGCCTCC 
AACACGCGCA AGGACCGACT GCTGGCCATG CAGGAGCAGT TCCGCCAGAT CGAGGAACTC 
TACTTGACAT TTGCCAAGAA AGCTTCGGCC TTCAATTCTT GGTTCGAAAA TGCCGAGGAG 
GATCTCACAG ATCCTGTTCG CTGCAATTCG ATCGAAGAAA TCCGCGCCCT GCGCGACGCC 
CATGCCCAAT TCCAGGCGTC CCTTTCGTCG GCCGAAGCTG ACTTCAAGGC ATTGGCAGCA 
CTCGACCAGA AGATCAAGAG CTTTAATGTT GGACCCAACC CGTACACGTG GTTCACCATG 
GAAGCTCTTG AGGAGACCTG GCGTAATCTG CAAAAGATCA TAGAGGAACG CGATGGGGAA 
CTTGCCAAGG AGGCCAAGCG GCAGGAGGAG AACGACAAGC TGCGCAAGGA GTTTGCCAAG 
CACGCCAATC TTTTCCACCA GTGGCTAACA GAGACAAGAA CGTCCATGAT GGAAGGCTCC 
GGTTCCCTGG AACAGCAACT GGAGGCGCTT CGTGTCAAGG CCACCGAGGT GCGCGCACGT 
CGCGTTGACC TCAAGAAGAT CGAGGAGCTG GGAGCCTTGC TGGAGGAGCA CCTTATTCTG 
GACAACCGCT ACACGGAACA CTCGACGGTG GGCCTGGCCC AGCAGTGGGA TCAGCTTGAC 
CAGCTGTCTA TGCGCATGCA GCACAACTTA GAGCAACAGA TCCAGGCACG CAACCACTCG 
GGCGTCTCCG AGGACTCACT CAAGGAGTTC TCGATGATGT TCAAGCACTT CGATAAGGAC 
AAGAGCGGCA AGCTCAACCA CCAGGAATTC AAGTCCTGCC TGCGCGCCCT CGGCTACGAC 
CTGCCCATGG TGGAGGAGGG ACAGCCCGAC CCGGAGTTCG AAGCAATTCT CGATGTTGTC 
GACCCCAACC GCGATGGCTA CGTCTCTCTG CAGGAATACA TCGCGTTCAT GATCTCCAAG 
GAGACGGAGA ACGTTCAATC CTACGAGGAG ATCGAGAATG CCTTCCGCGC CATCACCGCT 
GCCGACCGCC CCTATGTAAC TAAGGAGGAG CTCTACTGCA ACCTCACCAA GGACATGGCT 
GACTACTGCG TGCAGCGCAT GAAACCCTTC TCGGAACCGC GCTCCGGCCA GCCCATCAAG 
GATGCCCTGG ACTACATAGA CTTCACGCGA ACGCTGTTCC AGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

alpha-Spec ( NM_001274359.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261288.1 Drosophila melanogaster alpha spectrin, transcript variant D (alpha-Spec), mRNA.

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CloneID	OFa20437
Clone ID Related Accession (Same CDS sequence)	NM_001274359.1
Accession Version	NM_001274359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	alpha spectrin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAACT TTACACCCAA AGAGGTGAAG ATCCTCGAGA CAGTCGAGGA CATCCAGGAG 
CGACGTGAGC AGGTTCTGTC CCGCTACAAT GACTTCAAGA TCGAGACGCG CCAGAAGCGT 
GAAAAGCTCG AGGACTCGCG CCGCTTCCAG TACTTCAAGC GCGATGCCGA CGAGTTGGAG 
TCGTGGATCC ACGAGAAGCT GCAGGCAGCC AGCGAGGAGA GCTACCGCGA TCCGACCAAC 
CTGCAGGCCA AAATCCAGAA ACATCAGGCG TTCGAGGCGG AGGTGTCGGC GCACAGCAAC 
GCCATTGTCT CGCTGGACAA CACTGGCCAG GAGATGATCA ACCAGCAACA TTTTGCCTCC 
GAGTCGATTC AGGTCCGCCT CGACGAGCTG CACAAGCTGT GGGAGCTGCT TCTTAGCCGT 
CTGGCCGAGA AGGGTCTAAA GCTGCAGCAG GCTCTCGTCC TGGTGCAGTT CCTGCGCCAG 
TGCGAGGAGG TGATGTTCTG GATTAAGGAC AAGGAGACCT TTGTCACCGC CGACGAGTTC 
GGCCAAGACC TGGAACATGT AGAGGTTCTG CAGCGCAAGT TCGATGAGTT CCAGAAGGAC 
ATGGCCTCCC AGGAGTATCG CGTGACTGAG GTCAACCAGT TGGCCGATAA GTTGGTGCAA 
GATGGTCATC CCGAGCGCGA CACTATTACC AAGCGCAAGG AGGAGCTAAA CGAGGCTTGG 
CAGCGACTTA AGCAGTTAGC CATTGTGCGC CAAGAGAAGC TATTCGGTGC CCACGAGATC 
CAGCGTTTCA ATCGCGATGC CGACGAAACC GTCGCCTGGA TTGCCGAGAA GGATGTGGTC 
CTGTCGTCCG ATGATTATGG CCGCGATTTG GCCAGTGTTC AGGCGCTGCA ACGCAAACAC 
GAAGGAGTGG AGCGGGATTT GGCCGCCTTG GAGGATAAGG TTTCCACACT GGGAGCCGAG 
GCCCAGCGGC TGTGCTCCAT TCACGCCGAT CACAGTGACC AGATCCGTGA CAAACAGGCC 
GAGATTGCCA ACTACTGGCA GAGTCTCACC ACCAAGGCTC GCGAGCGCAA GCAGAAGCTA 
GATGAGTCCT ACTACCTGCA CCGTTTCCTC GCCGATTTCC GCGACCTCGT GTCCTGGATC 
AATGGCATGA AGGCCATCAT CTCGGCCGAT GAGCTGGCTA AGGATGTGGC TGGAGCAGAG 
GCTCTTCTGG AACGTCACCA GGAGCACAAG GGCGAGATCG ACGCTCGTGA GGATAGCTTC 
AAGCTGACCA CTGAATCCGG ACAGAAGCTG CTGGAGCGTG AGCATTACGC CGCCGCCGAA 
ATCCAGGAAA AGCTTGCCGC TCTGGAAAAC GACAAGAGTT CACTGCTCTC GCTGTGGGAA 
GATCGTCGCA TCCTGTACGA GCAGTGCATG GATCTGCAAC TGTTTTACCG CGATACGGAG 
CAGGCTGACA CTTGGATGGC CAAACAGGAG GCCTTCCTGG CCAACGAGGA CCTTGGCGAC 
TCCCTGGACT CTGTGGAGGC GCTAATTAAG AAGCACGAGG ACTTCGAGAA GAGTCTGGCT 
GCCCAGGAGG AGAAGATCAA GGCTTTAGAC ATCTTTGCCA CCAAATTAAT CGATGGTCAG 
CATTATGCCG CCGATGATGT GGCCCAGCGC CGTCAGATGC TCCTAGCCCG GCGGGCTGCT 
CTCCAAGAAA AATCGTCCAA GCGTCGCCAA TTGCTGGAGG ATTCGAACCG CTACCAGCAG 
TTCGAGCGCG ATTGCGACGA GACCAAGGGC TGGATTAGCG AGAAGCTGAA GTTTGCCACC 
GATGACAGCT ATTTGGATCC TACCAACCTT AACGGAAAGA TGCAGAAGCA CCAGAACTTT 
GAGCACGAGC TGAATGCCAA CAAGTCGCGC ATCGAGGACA TCACCAACGT GGGCACCGAG 
CTGATCGAGA AGCAGCACTA TGCTGCCGAC CAGATCAACA CCCGCATGCA GGAGATCGTC 
GTCCTGTGGG AGACCCTCGT CCAGGCTTCC GATAAAAAGG GAACCAAACT CAACGAGGCA 
TGCCAACAGC AGCAGTTCAA CCGCACCATC GAGGACATTG AGCTGTGGCT TAGCGAGATC 
GAGGGTCAAT TGTTGTCGGA GGATCACGGC AAAGACCTGA CCTCCGTTCA GAATTTGCAG 
AAGAAGCACG CCCTGCTGGA GGCCGATGTG ATGGCTCACC AGGATCGCAT TGAAAGCATC 
AAAGTGGCCG CCAACAAGTT CATCGAATCC GGTCACTTTG ACGCGGACAA CATTCGCAAC 
AAGGAGGGCA ATCTGTCGGC TCGCTATGCC GCCCTAGCTG CACCCATGGG CGAGAGGAAA 
CAACACCTTC TTGACTCCCT TCAGGTGCAG CAGCTCTTCC GCGACTTGGA GGACGAGGCT 
GCCTGGATCC GTGAGAAGGA ACCCATTGCC GCATCCACGA ACCGCGGTCG TGACTTGATT 
GGTGTGCAAA ACCTGATCAA GAAACACCAG GCTGTGCTGG CCGAGATCAA CAACCATGAG 
GCCAGGCTGC TGAATGTGAT CAGTTCGGGC GAGAACATGC TGAAGGACCA GCCCTTCGCC 
AGCGACGATA TCCGTCAGCG TTTGGAGGCG CTCCAAGAGC AGTGGAACAC GCTGAAGGAG 
AAGTCCAGCC AGCGAAAGCA GGATCTGGAT GACTCTCTCC AGGCACACCA GTACTTTGCC 
GACGCCAACG AAGCTGAGTC GTGGATGCGC GAAAAGGAAC CGATTGCAAC TGGCAGTGAT 
TATGGTAAGG ACGAGGATTC CTCGGAGGCT TTGCTCAAGA AGCACGAGGC TCTGGTCTCC 
GATCTAGAGG CTTTTGGCAA CACCATTCAG GCGCTGCAGG AGCAGGCCAA GAACTGTCGC 
CAGCAGGAGA CCCCTGTTGT GGATATCACT GGCAAGGAGT GCGTGGTTGC CCTCTACGAC 
TACACGGAGA AATCCCCTCG TGAGGTCTCG ATGAAGAAGG GCGATGTTCT GACCCTGCTG 
AACTCGAACA ACAAAGACTG GTGGAAGGTG GAGGTCAACG ACCGCCAGGG CTTCGTACCG 
GCCGCATACA TCAAGAAGAT TGACGCCGGC TTGAGCGCCT CCCAGCAGAA CCTAGTGGAC 
AATCATTCGA TTGCCAAGCG CCAGAACCAG ATCAACTCGC AGTACGACAA CTTGCTGGCC 
CTCGCCCGCG AGCGCCAGAA CAAGCTAAAC GAGACTGTGA AGGCGTACGT TCTTGTTCGT 
GAGGCTGCCG ACTTGGCCCA GTGGATCCGG GACAAGGAGA ACCACGCTCA GATCGCTGAT 
GTTGTGGGAG AGGATCTGGA AGAGGTGGAG GTGCTCCAAA AGAAGTTCGA CGACTTCAAC 
GACGACCTGA AGGCCAACGA AGTGCGTCTG GCAAACATGA ACGAGATTGC CGTGCAGCTG 
ACTTCCTTGG GTCAGACCGA GGCCGCCCTA AAGATCCAAA CCCAGATGCA AGACCTGAAC 
GAGAAGTGGA ACAACCTGCA GACGCTGACC GCTGAGAAGG CCAGCCAACT GGGATCCGCC 
CATGAGGTGC AACGCTTCCA TCGTGACATT GACGAGACCA AGGATTGGAT CGCTGAGAAG 
GCCAATGCCC TGAACAACGA CGACCTGGGC AAGGATCTGC GCAGTGTCCA GACTCTGCAG 
CGCAAGCACG AGGGCGTGGA ACGCGACTTG GCCGCATTGC GGGACAAGAT CCGCCAGCTG 
GACGAAACCG CCAACAGGCT GATGCAGTCG CACCCGGACA CCGCCGAGCA GACCTACGCC 
AAGCAGAAGG AGATCAACGA GATGTGGGAC CAGATCATCA CCAAGTCCAC CGCCCGCAAG 
GAGAAGCTGC TGGACTCGTA CGACCTGCAG CGTTTCCTCA GCGACTACCG CGATCTGCTG 
GCCTGGATCA ACTCGATGAT GAGCCTGGTC ACGTCCGACG AGCTGGCCAA CGATGTAACA 
GGAGCCGAGG CTCTGATCGA GCGTCATCAG GAACACCGAA CGGAGATCGA TGCGCGCGCC 
GGAACTTTCG GGGCTTTCGA GCAGTTCGGA AACGAGCTGC TGCAGGCCAA CCACTATGCG 
TCGCCTGAAA TCAAGGAGAA AATCGAGGAT CTGGCCAAGG CCCGCGAGGA CTTGGAGAAG 
GCGTGGACAG AGCGCCGCCT GCAGCTGGAG CAGAACTTGG ATCTGCAGCT CTACATGCGT 
GACTGCGAGT TGGCCGAGTC CTGGATGTCA GCCCGCGAGG CCTTCCTAAA TGCCGATGAT 
GATGCCAATG CCGGCGGAAA TGTGGAGGCA CTGATCAAGA AGCACGAGGA CTTCGACAAG 
GCCATTAACG GCCACGAACA GAAGATTGCT GCTCTGCAGA CGGTGGCGGA TCAGTTGATC 
GCCCAGAACC ACTATGCCTC CAATTTGGTG GACGAGAAGC GCAAGCAAGT GCTGGAGCGC 
TGGCGCCATC TCAAGGAGGG TCTGATCGAG AAGCGATCCC GTTTGGGTGA CGAGCAGACG 
CTGCAGCAGT TCTCGCGCGA TGCCGATGAG ATTGAGAACT GGATTGCAGA GAAGTTGCAG 
CTGGCCACCG AAGAGAGCTA CAAGGACCCG GCTAACATTC AGTCGAAGCA CCAGAAGCAC 
CAGGCCTTCG AGGCGGAGCT GGCGGCTAAC GCCGATCGCA TCCAGAGCGT GCTAGCCATG 
GGCGGAAACC TCATCGATAA GAAGCAGTGC AGCGGGTCGG AGGACGCGGT GCAGAAGCGG 
CTGACGCAGA TCGCCGATCA GTGGGAGTAC CTGACCCATA AGACTACCGA GAAGTCGCTG 
AAGCTAAAGG AGGCGAATAA GCAGCGCACC TATATCGCTG CTGTCAAGGA TTTGGACTTC 
TGGTTGGGCG AGGTCGAGAG TCTTTTGACC ACTGAGGATT CTGGTAAGGA TTTGGCCTCT 
GTCCAAAACC TCATGAAGAA GCATCAGTTG GTCGAGGCGG ATATCGTGGC GCACGAAGAC 
CGCATCAAGG ACATGAACAA CCAGGCCGAT TCCTTGGTGG AGAGCGGCCA GTTCGATACT 
GCCGGCATCC AGGAGAAGCG TCAAAGCATC AATGAGCGCT ACGAGCGCAT CTGCAACCTG 
GCGGCCCATC GCCAAGCCCG TCTCAACGAG GCTCTGACCC TGCACCAATT CTTCCGCGAC 
ATCGCCGACG AGGAGAGCTG GATCAAGGAG AAGAAGCTAC TTGTCGGCTC CGACGACTAT 
GGTCGCGATC TGACCGGCGT CCAGAACCTC AAGAAGAAGC ACAAGCGCCT AGAAGCCGAA 
TTGGGCTCTC ACGAGCCGGC CATTCAAGCT GTGCAGGAGG CTGGCGAGAA GCTCATGGAC 
GTGTCCAACC TCGGTGTCCC CGAAATTGAG CAGCGCCTGA AGGCTCTGAA CCAGGCCTGG 
GCCGAGCTAA AGAACCTGGC TGCCACGCGA GGTCAGAAGT TGGATGAGTC GCTGACCTAT 
CAGCAATTCC TCGCTCAGGT GGAGGAGGAG GAGGCCTGGA TCACCGAGAA GCAACAGCTG 
CTCTCTGTGG AGGACTACGG CGACTCGATG GCCGCCGTAC AAGGACTGCT GAAAAAGCAC 
GACGCATTCG AAACGGACTT CACTGCCCAC AAGGACCGCT GTTCTTTGAT CTGCGACCAG 
GGCAGTGAGT TGGTGGAGGC CAAGAACCAC CACGGAGAGT CCATTGCCCA ACGCTGCCAG 
CAGCTGCGCC TGAAACTGGA CAACCTGTCC GCCCTGGCTG CCCGCCGCAA GGGTGCCCTG 
CTGGACAACT CGGCCTATCT GCAGTTCATG TGGAAGGCCG ATGTGGTCGA GAGCTGGATC 
GACGACAAGG AGAACTATGT GCGCTCCGAT GAGTTCGGAC GCGACCTGTC CACCGTGCAA 
ACGCTGTTGA CCAAGCAAGA GACATTCGAT GCAGGTCTCA ATGCCTTCGA GCAGGAGGGC 
ATACACAACA TCACGGCACT TAAGGACCAA CTGATAAATG CCAGTCACGC CCAGTCGCCG 
GCAATCCTAA AGCGTCACGG AGACGTGATT GCCAGGTGGC AGAAGTTGCG CGACGCCTCC 
AACACGCGCA AGGACCGACT GCTGGCCATG CAGGAGCAGT TCCGCCAGAT CGAGGAACTC 
TACTTGACAT TTGCCAAGAA AGCTTCGGCC TTCAATTCTT GGTTCGAAAA TGCCGAGGAG 
GATCTCACAG ATCCTGTTCG CTGCAATTCG ATCGAAGAAA TCCGCGCCCT GCGCGACGCC 
CATGCCCAAT TCCAGGCGTC CCTTTCGTCG GCCGAAGCTG ACTTCAAGGC ATTGGCAGCA 
CTCGACCAGA AGATCAAGAG CTTTAATGTT GGACCCAACC CGTACACGTG GTTCACCATG 
GAAGCTCTTG AGGAGACCTG GCGTAATCTG CAAAAGATCA TAGAGGAACG CGATGGGGAA 
CTTGCCAAGG AGGCCAAGCG GCAGGAGGAG AACGACAAGC TGCGCAAGGA GTTTGCCAAG 
CACGCCAATC TTTTCCACCA GTGGCTAACA GAGACAAGAT ATTATATGCT GGGTTATAAC 
CGTCAAGGAA CGTCCATGAT GGAAGGCTCC GGTTCCCTGG AACAGCAACT GGAGGCGCTT 
CGTGTCAAGG CCACCGAGGT GCGCGCACGT CGCGTTGACC TCAAGAAGAT CGAGGAGCTG 
GGAGCCTTGC TGGAGGAGCA CCTTATTCTG GACAACCGCT ACACGGAACA CTCGACGGTG 
GGCCTGGCCC AGCAGTGGGA TCAGCTTGAC CAGCTGTCTA TGCGCATGCA GCACAACTTA 
GAGCAACAGA TCCAGGCACG CAACCACTCG GGCGTCTCCG AGGACTCACT CAAGGAGTTC 
TCGATGATGT TCAAGCACTT CGATAAGGAC AAGAGCGGCA AGCTCAACCA CCAGGAATTC 
AAGTCCTGCC TGCGCGCCCT CGGCTACGAC CTGCCCATGG TGGAGGAGGG ACAGCCCGAC 
CCGGAGTTCG AAGCAATTCT CGATGTTGTC GACCCCAACC GCGATGGCTA CGTCTCTCTG 
CAGGAATACA TCGCGTTCAT GATCTCCAAG GAGACGGAGA ACGTTCAATC CTACGAGGAG 
ATCGAGAATG CCTTCCGCGC CATCACCGCT GCCGACCGCC CCTATGTAAC TAAGGAGGAG 
CTCTACTGCA ACCTCACCAA GGACATGGCT GACTACTGCG TGCAGCGCAT GAAACCCTTC 
TCGGAACCGC GCTCCGGCCA GCCCATCAAG GATGCCCTGG ACTACATAGA CTTCACGCGA 
ACGCTGTTCC AGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261288.1
CDS	277..7554
Translation	MENFTPKEVKILETVEDIQERREQVLSRYNDFKIETRQKREKLEDSRRFQYFKRDADELESWIHEKLQAASEESYRDPTNLQAKIQKHQAFEAEVSAHSNAIVSLDNTGQEMINQQHFASESIQVRLDELHKLWELLLSRLAEKGLKLQQALVLVQFLRQCEEVMFWIKDKETFVTADEFGQDLEHVEVLQRKFDEFQKDMASQEYRVTEVNQLADKLVQDGHPERDTITKRKEELNEAWQRLKQLAIVRQEKLFGAHEIQRFNRDADETVAWIAEKDVVLSSDDYGRDLASVQALQRKHEGVERDLAALEDKVSTLGAEAQRLCSIHADHSDQIRDKQAEIANYWQSLTTKARERKQKLDESYYLHRFLADFRDLVSWINGMKAIISADELAKDVAGAEALLERHQEHKGEIDAREDSFKLTTESGQKLLEREHYAAAEIQEKLAALENDKSSLLSLWEDRRILYEQCMDLQLFYRDTEQADTWMAKQEAFLANEDLGDSLDSVEALIKKHEDFEKSLAAQEEKIKALDIFATKLIDGQHYAADDVAQRRQMLLARRAALQEKSSKRRQLLEDSNRYQQFERDCDETKGWISEKLKFATDDSYLDPTNLNGKMQKHQNFEHELNANKSRIEDITNVGTELIEKQHYAADQINTRMQEIVVLWETLVQASDKKGTKLNEACQQQQFNRTIEDIELWLSEIEGQLLSEDHGKDLTSVQNLQKKHALLEADVMAHQDRIESIKVAANKFIESGHFDADNIRNKEGNLSARYAALAAPMGERKQHLLDSLQVQQLFRDLEDEAAWIREKEPIAASTNRGRDLIGVQNLIKKHQAVLAEINNHEARLLNVISSGENMLKDQPFASDDIRQRLEALQEQWNTLKEKSSQRKQDLDDSLQAHQYFADANEAESWMREKEPIATGSDYGKDEDSSEALLKKHEALVSDLEAFGNTIQALQEQAKNCRQQETPVVDITGKECVVALYDYTEKSPREVSMKKGDVLTLLNSNNKDWWKVEVNDRQGFVPAAYIKKIDAGLSASQQNLVDNHSIAKRQNQINSQYDNLLALARERQNKLNETVKAYVLVREAADLAQWIRDKENHAQIADVVGEDLEEVEVLQKKFDDFNDDLKANEVRLANMNEIAVQLTSLGQTEAALKIQTQMQDLNEKWNNLQTLTAEKASQLGSAHEVQRFHRDIDETKDWIAEKANALNNDDLGKDLRSVQTLQRKHEGVERDLAALRDKIRQLDETANRLMQSHPDTAEQTYAKQKEINEMWDQIITKSTARKEKLLDSYDLQRFLSDYRDLLAWINSMMSLVTSDELANDVTGAEALIERHQEHRTEIDARAGTFGAFEQFGNELLQANHYASPEIKEKIEDLAKAREDLEKAWTERRLQLEQNLDLQLYMRDCELAESWMSAREAFLNADDDANAGGNVEALIKKHEDFDKAINGHEQKIAALQTVADQLIAQNHYASNLVDEKRKQVLERWRHLKEGLIEKRSRLGDEQTLQQFSRDADEIENWIAEKLQLATEESYKDPANIQSKHQKHQAFEAELAANADRIQSVLAMGGNLIDKKQCSGSEDAVQKRLTQIADQWEYLTHKTTEKSLKLKEANKQRTYIAAVKDLDFWLGEVESLLTTEDSGKDLASVQNLMKKHQLVEADIVAHEDRIKDMNNQADSLVESGQFDTAGIQEKRQSINERYERICNLAAHRQARLNEALTLHQFFRDIADEESWIKEKKLLVGSDDYGRDLTGVQNLKKKHKRLEAELGSHEPAIQAVQEAGEKLMDVSNLGVPEIEQRLKALNQAWAELKNLAATRGQKLDESLTYQQFLAQVEEEEAWITEKQQLLSVEDYGDSMAAVQGLLKKHDAFETDFTAHKDRCSLICDQGSELVEAKNHHGESIAQRCQQLRLKLDNLSALAARRKGALLDNSAYLQFMWKADVVESWIDDKENYVRSDEFGRDLSTVQTLLTKQETFDAGLNAFEQEGIHNITALKDQLINASHAQSPAILKRHGDVIARWQKLRDASNTRKDRLLAMQEQFRQIEELYLTFAKKASAFNSWFENAEEDLTDPVRCNSIEEIRALRDAHAQFQASLSSAEADFKALAALDQKIKSFNVGPNPYTWFTMEALEETWRNLQKIIEERDGELAKEAKRQEENDKLRKEFAKHANLFHQWLTETRYYMLGYNRQGTSMMEGSGSLEQQLEALRVKATEVRARRVDLKKIEELGALLEEHLILDNRYTEHSTVGLAQQWDQLDQLSMRMQHNLEQQIQARNHSGVSEDSLKEFSMMFKHFDKDKSGKLNHQEFKSCLRALGYDLPMVEEGQPDPEFEAILDVVDPNRDGYVSLQEYIAFMISKETENVQSYEEIENAFRAITAADRPYVTKEELYCNLTKDMADYCVQRMKPFSEPRSGQPIKDALDYIDFTRTLFQN

Target ORF information:

RefSeq Version	NM_001274359.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alpha spectrin, transcript variant D (alpha-Spec), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274359.1

ORF Insert Sequence:

ATGGAGAACT TTACACCCAA AGAGGTGAAG ATCCTCGAGA CAGTCGAGGA CATCCAGGAG 
CGACGTGAGC AGGTTCTGTC CCGCTACAAT GACTTCAAGA TCGAGACGCG CCAGAAGCGT 
GAAAAGCTCG AGGACTCGCG CCGCTTCCAG TACTTCAAGC GCGATGCCGA CGAGTTGGAG 
TCGTGGATCC ACGAGAAGCT GCAGGCAGCC AGCGAGGAGA GCTACCGCGA TCCGACCAAC 
CTGCAGGCCA AAATCCAGAA ACATCAGGCG TTCGAGGCGG AGGTGTCGGC GCACAGCAAC 
GCCATTGTCT CGCTGGACAA CACTGGCCAG GAGATGATCA ACCAGCAACA TTTTGCCTCC 
GAGTCGATTC AGGTCCGCCT CGACGAGCTG CACAAGCTGT GGGAGCTGCT TCTTAGCCGT 
CTGGCCGAGA AGGGTCTAAA GCTGCAGCAG GCTCTCGTCC TGGTGCAGTT CCTGCGCCAG 
TGCGAGGAGG TGATGTTCTG GATTAAGGAC AAGGAGACCT TTGTCACCGC CGACGAGTTC 
GGCCAAGACC TGGAACATGT AGAGGTTCTG CAGCGCAAGT TCGATGAGTT CCAGAAGGAC 
ATGGCCTCCC AGGAGTATCG CGTGACTGAG GTCAACCAGT TGGCCGATAA GTTGGTGCAA 
GATGGTCATC CCGAGCGCGA CACTATTACC AAGCGCAAGG AGGAGCTAAA CGAGGCTTGG 
CAGCGACTTA AGCAGTTAGC CATTGTGCGC CAAGAGAAGC TATTCGGTGC CCACGAGATC 
CAGCGTTTCA ATCGCGATGC CGACGAAACC GTCGCCTGGA TTGCCGAGAA GGATGTGGTC 
CTGTCGTCCG ATGATTATGG CCGCGATTTG GCCAGTGTTC AGGCGCTGCA ACGCAAACAC 
GAAGGAGTGG AGCGGGATTT GGCCGCCTTG GAGGATAAGG TTTCCACACT GGGAGCCGAG 
GCCCAGCGGC TGTGCTCCAT TCACGCCGAT CACAGTGACC AGATCCGTGA CAAACAGGCC 
GAGATTGCCA ACTACTGGCA GAGTCTCACC ACCAAGGCTC GCGAGCGCAA GCAGAAGCTA 
GATGAGTCCT ACTACCTGCA CCGTTTCCTC GCCGATTTCC GCGACCTCGT GTCCTGGATC 
AATGGCATGA AGGCCATCAT CTCGGCCGAT GAGCTGGCTA AGGATGTGGC TGGAGCAGAG 
GCTCTTCTGG AACGTCACCA GGAGCACAAG GGCGAGATCG ACGCTCGTGA GGATAGCTTC 
AAGCTGACCA CTGAATCCGG ACAGAAGCTG CTGGAGCGTG AGCATTACGC CGCCGCCGAA 
ATCCAGGAAA AGCTTGCCGC TCTGGAAAAC GACAAGAGTT CACTGCTCTC GCTGTGGGAA 
GATCGTCGCA TCCTGTACGA GCAGTGCATG GATCTGCAAC TGTTTTACCG CGATACGGAG 
CAGGCTGACA CTTGGATGGC CAAACAGGAG GCCTTCCTGG CCAACGAGGA CCTTGGCGAC 
TCCCTGGACT CTGTGGAGGC GCTAATTAAG AAGCACGAGG ACTTCGAGAA GAGTCTGGCT 
GCCCAGGAGG AGAAGATCAA GGCTTTAGAC ATCTTTGCCA CCAAATTAAT CGATGGTCAG 
CATTATGCCG CCGATGATGT GGCCCAGCGC CGTCAGATGC TCCTAGCCCG GCGGGCTGCT 
CTCCAAGAAA AATCGTCCAA GCGTCGCCAA TTGCTGGAGG ATTCGAACCG CTACCAGCAG 
TTCGAGCGCG ATTGCGACGA GACCAAGGGC TGGATTAGCG AGAAGCTGAA GTTTGCCACC 
GATGACAGCT ATTTGGATCC TACCAACCTT AACGGAAAGA TGCAGAAGCA CCAGAACTTT 
GAGCACGAGC TGAATGCCAA CAAGTCGCGC ATCGAGGACA TCACCAACGT GGGCACCGAG 
CTGATCGAGA AGCAGCACTA TGCTGCCGAC CAGATCAACA CCCGCATGCA GGAGATCGTC 
GTCCTGTGGG AGACCCTCGT CCAGGCTTCC GATAAAAAGG GAACCAAACT CAACGAGGCA 
TGCCAACAGC AGCAGTTCAA CCGCACCATC GAGGACATTG AGCTGTGGCT TAGCGAGATC 
GAGGGTCAAT TGTTGTCGGA GGATCACGGC AAAGACCTGA CCTCCGTTCA GAATTTGCAG 
AAGAAGCACG CCCTGCTGGA GGCCGATGTG ATGGCTCACC AGGATCGCAT TGAAAGCATC 
AAAGTGGCCG CCAACAAGTT CATCGAATCC GGTCACTTTG ACGCGGACAA CATTCGCAAC 
AAGGAGGGCA ATCTGTCGGC TCGCTATGCC GCCCTAGCTG CACCCATGGG CGAGAGGAAA 
CAACACCTTC TTGACTCCCT TCAGGTGCAG CAGCTCTTCC GCGACTTGGA GGACGAGGCT 
GCCTGGATCC GTGAGAAGGA ACCCATTGCC GCATCCACGA ACCGCGGTCG TGACTTGATT 
GGTGTGCAAA ACCTGATCAA GAAACACCAG GCTGTGCTGG CCGAGATCAA CAACCATGAG 
GCCAGGCTGC TGAATGTGAT CAGTTCGGGC GAGAACATGC TGAAGGACCA GCCCTTCGCC 
AGCGACGATA TCCGTCAGCG TTTGGAGGCG CTCCAAGAGC AGTGGAACAC GCTGAAGGAG 
AAGTCCAGCC AGCGAAAGCA GGATCTGGAT GACTCTCTCC AGGCACACCA GTACTTTGCC 
GACGCCAACG AAGCTGAGTC GTGGATGCGC GAAAAGGAAC CGATTGCAAC TGGCAGTGAT 
TATGGTAAGG ACGAGGATTC CTCGGAGGCT TTGCTCAAGA AGCACGAGGC TCTGGTCTCC 
GATCTAGAGG CTTTTGGCAA CACCATTCAG GCGCTGCAGG AGCAGGCCAA GAACTGTCGC 
CAGCAGGAGA CCCCTGTTGT GGATATCACT GGCAAGGAGT GCGTGGTTGC CCTCTACGAC 
TACACGGAGA AATCCCCTCG TGAGGTCTCG ATGAAGAAGG GCGATGTTCT GACCCTGCTG 
AACTCGAACA ACAAAGACTG GTGGAAGGTG GAGGTCAACG ACCGCCAGGG CTTCGTACCG 
GCCGCATACA TCAAGAAGAT TGACGCCGGC TTGAGCGCCT CCCAGCAGAA CCTAGTGGAC 
AATCATTCGA TTGCCAAGCG CCAGAACCAG ATCAACTCGC AGTACGACAA CTTGCTGGCC 
CTCGCCCGCG AGCGCCAGAA CAAGCTAAAC GAGACTGTGA AGGCGTACGT TCTTGTTCGT 
GAGGCTGCCG ACTTGGCCCA GTGGATCCGG GACAAGGAGA ACCACGCTCA GATCGCTGAT 
GTTGTGGGAG AGGATCTGGA AGAGGTGGAG GTGCTCCAAA AGAAGTTCGA CGACTTCAAC 
GACGACCTGA AGGCCAACGA AGTGCGTCTG GCAAACATGA ACGAGATTGC CGTGCAGCTG 
ACTTCCTTGG GTCAGACCGA GGCCGCCCTA AAGATCCAAA CCCAGATGCA AGACCTGAAC 
GAGAAGTGGA ACAACCTGCA GACGCTGACC GCTGAGAAGG CCAGCCAACT GGGATCCGCC 
CATGAGGTGC AACGCTTCCA TCGTGACATT GACGAGACCA AGGATTGGAT CGCTGAGAAG 
GCCAATGCCC TGAACAACGA CGACCTGGGC AAGGATCTGC GCAGTGTCCA GACTCTGCAG 
CGCAAGCACG AGGGCGTGGA ACGCGACTTG GCCGCATTGC GGGACAAGAT CCGCCAGCTG 
GACGAAACCG CCAACAGGCT GATGCAGTCG CACCCGGACA CCGCCGAGCA GACCTACGCC 
AAGCAGAAGG AGATCAACGA GATGTGGGAC CAGATCATCA CCAAGTCCAC CGCCCGCAAG 
GAGAAGCTGC TGGACTCGTA CGACCTGCAG CGTTTCCTCA GCGACTACCG CGATCTGCTG 
GCCTGGATCA ACTCGATGAT GAGCCTGGTC ACGTCCGACG AGCTGGCCAA CGATGTAACA 
GGAGCCGAGG CTCTGATCGA GCGTCATCAG GAACACCGAA CGGAGATCGA TGCGCGCGCC 
GGAACTTTCG GGGCTTTCGA GCAGTTCGGA AACGAGCTGC TGCAGGCCAA CCACTATGCG 
TCGCCTGAAA TCAAGGAGAA AATCGAGGAT CTGGCCAAGG CCCGCGAGGA CTTGGAGAAG 
GCGTGGACAG AGCGCCGCCT GCAGCTGGAG CAGAACTTGG ATCTGCAGCT CTACATGCGT 
GACTGCGAGT TGGCCGAGTC CTGGATGTCA GCCCGCGAGG CCTTCCTAAA TGCCGATGAT 
GATGCCAATG CCGGCGGAAA TGTGGAGGCA CTGATCAAGA AGCACGAGGA CTTCGACAAG 
GCCATTAACG GCCACGAACA GAAGATTGCT GCTCTGCAGA CGGTGGCGGA TCAGTTGATC 
GCCCAGAACC ACTATGCCTC CAATTTGGTG GACGAGAAGC GCAAGCAAGT GCTGGAGCGC 
TGGCGCCATC TCAAGGAGGG TCTGATCGAG AAGCGATCCC GTTTGGGTGA CGAGCAGACG 
CTGCAGCAGT TCTCGCGCGA TGCCGATGAG ATTGAGAACT GGATTGCAGA GAAGTTGCAG 
CTGGCCACCG AAGAGAGCTA CAAGGACCCG GCTAACATTC AGTCGAAGCA CCAGAAGCAC 
CAGGCCTTCG AGGCGGAGCT GGCGGCTAAC GCCGATCGCA TCCAGAGCGT GCTAGCCATG 
GGCGGAAACC TCATCGATAA GAAGCAGTGC AGCGGGTCGG AGGACGCGGT GCAGAAGCGG 
CTGACGCAGA TCGCCGATCA GTGGGAGTAC CTGACCCATA AGACTACCGA GAAGTCGCTG 
AAGCTAAAGG AGGCGAATAA GCAGCGCACC TATATCGCTG CTGTCAAGGA TTTGGACTTC 
TGGTTGGGCG AGGTCGAGAG TCTTTTGACC ACTGAGGATT CTGGTAAGGA TTTGGCCTCT 
GTCCAAAACC TCATGAAGAA GCATCAGTTG GTCGAGGCGG ATATCGTGGC GCACGAAGAC 
CGCATCAAGG ACATGAACAA CCAGGCCGAT TCCTTGGTGG AGAGCGGCCA GTTCGATACT 
GCCGGCATCC AGGAGAAGCG TCAAAGCATC AATGAGCGCT ACGAGCGCAT CTGCAACCTG 
GCGGCCCATC GCCAAGCCCG TCTCAACGAG GCTCTGACCC TGCACCAATT CTTCCGCGAC 
ATCGCCGACG AGGAGAGCTG GATCAAGGAG AAGAAGCTAC TTGTCGGCTC CGACGACTAT 
GGTCGCGATC TGACCGGCGT CCAGAACCTC AAGAAGAAGC ACAAGCGCCT AGAAGCCGAA 
TTGGGCTCTC ACGAGCCGGC CATTCAAGCT GTGCAGGAGG CTGGCGAGAA GCTCATGGAC 
GTGTCCAACC TCGGTGTCCC CGAAATTGAG CAGCGCCTGA AGGCTCTGAA CCAGGCCTGG 
GCCGAGCTAA AGAACCTGGC TGCCACGCGA GGTCAGAAGT TGGATGAGTC GCTGACCTAT 
CAGCAATTCC TCGCTCAGGT GGAGGAGGAG GAGGCCTGGA TCACCGAGAA GCAACAGCTG 
CTCTCTGTGG AGGACTACGG CGACTCGATG GCCGCCGTAC AAGGACTGCT GAAAAAGCAC 
GACGCATTCG AAACGGACTT CACTGCCCAC AAGGACCGCT GTTCTTTGAT CTGCGACCAG 
GGCAGTGAGT TGGTGGAGGC CAAGAACCAC CACGGAGAGT CCATTGCCCA ACGCTGCCAG 
CAGCTGCGCC TGAAACTGGA CAACCTGTCC GCCCTGGCTG CCCGCCGCAA GGGTGCCCTG 
CTGGACAACT CGGCCTATCT GCAGTTCATG TGGAAGGCCG ATGTGGTCGA GAGCTGGATC 
GACGACAAGG AGAACTATGT GCGCTCCGAT GAGTTCGGAC GCGACCTGTC CACCGTGCAA 
ACGCTGTTGA CCAAGCAAGA GACATTCGAT GCAGGTCTCA ATGCCTTCGA GCAGGAGGGC 
ATACACAACA TCACGGCACT TAAGGACCAA CTGATAAATG CCAGTCACGC CCAGTCGCCG 
GCAATCCTAA AGCGTCACGG AGACGTGATT GCCAGGTGGC AGAAGTTGCG CGACGCCTCC 
AACACGCGCA AGGACCGACT GCTGGCCATG CAGGAGCAGT TCCGCCAGAT CGAGGAACTC 
TACTTGACAT TTGCCAAGAA AGCTTCGGCC TTCAATTCTT GGTTCGAAAA TGCCGAGGAG 
GATCTCACAG ATCCTGTTCG CTGCAATTCG ATCGAAGAAA TCCGCGCCCT GCGCGACGCC 
CATGCCCAAT TCCAGGCGTC CCTTTCGTCG GCCGAAGCTG ACTTCAAGGC ATTGGCAGCA 
CTCGACCAGA AGATCAAGAG CTTTAATGTT GGACCCAACC CGTACACGTG GTTCACCATG 
GAAGCTCTTG AGGAGACCTG GCGTAATCTG CAAAAGATCA TAGAGGAACG CGATGGGGAA 
CTTGCCAAGG AGGCCAAGCG GCAGGAGGAG AACGACAAGC TGCGCAAGGA GTTTGCCAAG 
CACGCCAATC TTTTCCACCA GTGGCTAACA GAGACAAGAT ATTATATGCT GGGTTATAAC 
CGTCAAGGAA CGTCCATGAT GGAAGGCTCC GGTTCCCTGG AACAGCAACT GGAGGCGCTT 
CGTGTCAAGG CCACCGAGGT GCGCGCACGT CGCGTTGACC TCAAGAAGAT CGAGGAGCTG 
GGAGCCTTGC TGGAGGAGCA CCTTATTCTG GACAACCGCT ACACGGAACA CTCGACGGTG 
GGCCTGGCCC AGCAGTGGGA TCAGCTTGAC CAGCTGTCTA TGCGCATGCA GCACAACTTA 
GAGCAACAGA TCCAGGCACG CAACCACTCG GGCGTCTCCG AGGACTCACT CAAGGAGTTC 
TCGATGATGT TCAAGCACTT CGATAAGGAC AAGAGCGGCA AGCTCAACCA CCAGGAATTC 
AAGTCCTGCC TGCGCGCCCT CGGCTACGAC CTGCCCATGG TGGAGGAGGG ACAGCCCGAC 
CCGGAGTTCG AAGCAATTCT CGATGTTGTC GACCCCAACC GCGATGGCTA CGTCTCTCTG 
CAGGAATACA TCGCGTTCAT GATCTCCAAG GAGACGGAGA ACGTTCAATC CTACGAGGAG 
ATCGAGAATG CCTTCCGCGC CATCACCGCT GCCGACCGCC CCTATGTAAC TAAGGAGGAG 
CTCTACTGCA ACCTCACCAA GGACATGGCT GACTACTGCG TGCAGCGCAT GAAACCCTTC 
TCGGAACCGC GCTCCGGCCA GCCCATCAAG GATGCCCTGG ACTACATAGA CTTCACGCGA 
ACGCTGTTCC AGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

alpha-Spec ( NM_001274358.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261287.1 Drosophila melanogaster alpha spectrin, transcript variant C (alpha-Spec), mRNA.

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CloneID	OFa20438
Clone ID Related Accession (Same CDS sequence)	NM_001274358.1
Accession Version	NM_001274358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	alpha spectrin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAACT TTACACCCAA AGAGGTGAAG ATCCTCGAGA CAGTCGAGGA CATCCAGGAG 
CGACGTGAGC AGGTTCTGTC CCGCTACAAT GACTTCAAGA TCGAGACGCG CCAGAAGCGT 
GAAAAGCTCG AGGACTCGCG CCGCTTCCAG TACTTCAAGC GCGATGCCGA CGAGTTGGAG 
TCGTGGATCC ACGAGAAGCT GCAGGCAGCC AGCGAGGAGA GCTACCGCGA TCCGACCAAC 
CTGCAGGCCA AAATCCAGAA ACATCAGGCG TTCGAGGCGG AGGTGTCGGC GCACAGCAAC 
GCCATTGTCT CGCTGGACAA CACTGGCCAG GAGATGATCA ACCAGCAACA TTTTGCCTCC 
GAGTCGATTC AGGTCCGCCT CGACGAGCTG CACAAGCTGT GGGAGCTGCT TCTTAGCCGT 
CTGGCCGAGA AGGGTCTAAA GCTGCAGCAG GCTCTCGTCC TGGTGCAGTT CCTGCGCCAG 
TGCGAGGAGG TGATGTTCTG GATTAAGGAC AAGGAGACCT TTGTCACCGC CGACGAGTTC 
GGCCAAGACC TGGAACATGT AGAGGTTCTG CAGCGCAAGT TCGATGAGTT CCAGAAGGAC 
ATGGCCTCCC AGGAGTATCG CGTGACTGAG GTCAACCAGT TGGCCGATAA GTTGGTGCAA 
GATGGTCATC CCGAGCGCGA CACTATTACC AAGCGCAAGG AGGAGCTAAA CGAGGCTTGG 
CAGCGACTTA AGCAGTTAGC CATTGTGCGC CAAGAGAAGC TATTCGGTGC CCACGAGATC 
CAGCGTTTCA ATCGCGATGC CGACGAAACC GTCGCCTGGA TTGCCGAGAA GGATGTGGTC 
CTGTCGTCCG ATGATTATGG CCGCGATTTG GCCAGTGTTC AGGCGCTGCA ACGCAAACAC 
GAAGGAGTGG AGCGGGATTT GGCCGCCTTG GAGGATAAGG TTTCCACACT GGGAGCCGAG 
GCCCAGCGGC TGTGCTCCAT TCACGCCGAT CACAGTGACC AGATCCGTGA CAAACAGGCC 
GAGATTGCCA ACTACTGGCA GAGTCTCACC ACCAAGGCTC GCGAGCGCAA GCAGAAGCTA 
GATGAGTCCT ACTACCTGCA CCGTTTCCTC GCCGATTTCC GCGACCTCGT GTCCTGGATC 
AATGGCATGA AGGCCATCAT CTCGGCCGAT GAGCTGGCTA AGGATGTGGC TGGAGCAGAG 
GCTCTTCTGG AACGTCACCA GGAGCACAAG GGCGAGATCG ACGCTCGTGA GGATAGCTTC 
AAGCTGACCA CTGAATCCGG ACAGAAGCTG CTGGAGCGTG AGCATTACGC CGCCGCCGAA 
ATCCAGGAAA AGCTTGCCGC TCTGGAAAAC GACAAGAGTT CACTGCTCTC GCTGTGGGAA 
GATCGTCGCA TCCTGTACGA GCAGTGCATG GATCTGCAAC TGTTTTACCG CGATACGGAG 
CAGGCTGACA CTTGGATGGC CAAACAGGAG GCCTTCCTGG CCAACGAGGA CCTTGGCGAC 
TCCCTGGACT CTGTGGAGGC GCTAATTAAG AAGCACGAGG ACTTCGAGAA GAGTCTGGCT 
GCCCAGGAGG AGAAGATCAA GGCTTTAGAC ATCTTTGCCA CCAAATTAAT CGATGGTCAG 
CATTATGCCG CCGATGATGT GGCCCAGCGC CGTCAGATGC TCCTAGCCCG GCGGGCTGCT 
CTCCAAGAAA AATCGTCCAA GCGTCGCCAA TTGCTGGAGG ATTCGAACCG CTACCAGCAG 
TTCGAGCGCG ATTGCGACGA GACCAAGGGC TGGATTAGCG AGAAGCTGAA GTTTGCCACC 
GATGACAGCT ATTTGGATCC TACCAACCTT AACGGAAAGA TGCAGAAGCA CCAGAACTTT 
GAGCACGAGC TGAATGCCAA CAAGTCGCGC ATCGAGGACA TCACCAACGT GGGCACCGAG 
CTGATCGAGA AGCAGCACTA TGCTGCCGAC CAGATCAACA CCCGCATGCA GGAGATCGTC 
GTCCTGTGGG AGACCCTCGT CCAGGCTTCC GATAAAAAGG GAACCAAACT CAACGAGGCA 
TGCCAACAGC AGCAGTTCAA CCGCACCATC GAGGACATTG AGCTGTGGCT TAGCGAGATC 
GAGGGTCAAT TGTTGTCGGA GGATCACGGC AAAGACCTGA CCTCCGTTCA GAATTTGCAG 
AAGAAGCACG CCCTGCTGGA GGCCGATGTG ATGGCTCACC AGGATCGCAT TGAAAGCATC 
AAAGTGGCCG CCAACAAGTT CATCGAATCC GGTCACTTTG ACGCGGACAA CATTCGCAAC 
AAGGAGGGCA ATCTGTCGGC TCGCTATGCC GCCCTAGCTG CACCCATGGG CGAGAGGAAA 
CAACACCTTC TTGACTCCCT TCAGGTGCAG CAGCTCTTCC GCGACTTGGA GGACGAGGCT 
GCCTGGATCC GTGAGAAGGA ACCCATTGCC GCATCCACGA ACCGCGGTCG TGACTTGATT 
GGTGTGCAAA ACCTGATCAA GAAACACCAG GCTGTGCTGG CCGAGATCAA CAACCATGAG 
GCCAGGCTGC TGAATGTGAT CAGTTCGGGC GAGAACATGC TGAAGGACCA GCCCTTCGCC 
AGCGACGATA TCCGTCAGCG TTTGGAGGCG CTCCAAGAGC AGTGGAACAC GCTGAAGGAG 
AAGTCCAGCC AGCGAAAGCA GGATCTGGAT GACTCTCTCC AGGCACACCA GTACTTTGCC 
GACGCCAACG AAGCTGAGTC GTGGATGCGC GAAAAGGAAC CGATTGCAAC TGGCAGTGAT 
TATGGTAAGG ACGAGGATTC CTCGGAGGCT TTGCTCAAGA AGCACGAGGC TCTGGTCTCC 
GATCTAGAGG CTTTTGGCAA CACCATTCAG GCGCTGCAGG AGCAGGCCAA GAACTGTCGC 
CAGCAGGAGA CCCCTGTTGT GGATATCACT GGCAAGGAGT GCGTGGTTGC CCTCTACGAC 
TACACGGAGA AATCCCCTCG TGAGGTCTCG ATGAAGAAGG GCGATGTTCT GACCCTGCTG 
AACTCGAACA ACAAAGACTG GTGGAAGGTG GAGGTCAACG ACCGCCAGGG CTTCGTACCG 
GCCGCATACA TCAAGAAGAT TGACGCCGGC TTGAGCGCCT CCCAGCAGAA CCTAGTGGAC 
AATCATTCGA TTGCCAAGCG CCAGAACCAG ATCAACTCGC AGTACGACAA CTTGCTGGCC 
CTCGCCCGCG AGCGCCAGAA CAAGCTAAAC GAGACTGTGA AGGCGTACGT TCTTGTTCGT 
GAGGCTGCCG ACTTGGCCCA GTGGATCCGG GACAAGGAGA ACCACGCTCA GATCGCTGAT 
GTTGTGGGAG AGGATCTGGA AGAGGTGGAG GTGCTCCAAA AGAAGTTCGA CGACTTCAAC 
GACGACCTGA AGGCCAACGA AGTGCGTCTG GCAAACATGA ACGAGATTGC CGTGCAGCTG 
ACTTCCTTGG GTCAGACCGA GGCCGCCCTA AAGATCCAAA CCCAGATGCA AGACCTGAAC 
GAGAAGTGGA ACAACCTGCA GACGCTGACC GCTGAGAAGG CCAGCCAACT GGGATCCGCC 
CATGAGGTGC AACGCTTCCA TCGTGACATT GACGAGACCA AGGATTGGAT CGCTGAGAAG 
GCCAATGCCC TGAACAACGA CGACCTGGGC AAGGATCTGC GCAGTGTCCA GACTCTGCAG 
CGCAAGCACG AGGGCGTGGA ACGCGACTTG GCCGCATTGC GGGACAAGAT CCGCCAGCTG 
GACGAAACCG CCAACAGGCT GATGCAGTCG CACCCGGACA CCGCCGAGCA GACCTACGCC 
AAGCAGAAGG AGATCAACGA GATGTGGGAC CAGATCATCA CCAAGTCCAC CGCCCGCAAG 
GAGAAGCTGC TGGACTCGTA CGACCTGCAG CGTTTCCTCA GCGACTACCG CGATCTGCTG 
GCCTGGATCA ACTCGATGAT GAGCCTGGTC ACGTCCGACG AGCTGGCCAA CGATGTAACA 
GGAGCCGAGG CTCTGATCGA GCGTCATCAG GCACGCCGTG CCGAGATTGG GTTCACATTG 
GGCATCAGCT CGGCTCCCGG AGCCGCCGCC TCGACCTCCA GCATCGCATC TCCATCCGGC 
GACGAGGAAC ACCGAACGGA GATCGATGCG CGCGCCGGAA CTTTCGGGGC TTTCGAGCAG 
TTCGGAAACG AGCTGCTGCA GGCCAACCAC TATGCGTCGC CTGAAATCAA GGAGAAAATC 
GAGGATCTGG CCAAGGCCCG CGAGGACTTG GAGAAGGCGT GGACAGAGCG CCGCCTGCAG 
CTGGAGCAGA ACTTGGATCT GCAGCTCTAC ATGCGTGACT GCGAGTTGGC CGAGTCCTGG 
ATGTCAGCCC GCGAGGCCTT CCTAAATGCC GATGATGATG CCAATGCCGG CGGAAATGTG 
GAGGCACTGA TCAAGAAGCA CGAGGACTTC GACAAGGCCA TTAACGGCCA CGAACAGAAG 
ATTGCTGCTC TGCAGACGGT GGCGGATCAG TTGATCGCCC AGAACCACTA TGCCTCCAAT 
TTGGTGGACG AGAAGCGCAA GCAAGTGCTG GAGCGCTGGC GCCATCTCAA GGAGGGTCTG 
ATCGAGAAGC GATCCCGTTT GGGTGACGAG CAGACGCTGC AGCAGTTCTC GCGCGATGCC 
GATGAGATTG AGAACTGGAT TGCAGAGAAG TTGCAGCTGG CCACCGAAGA GAGCTACAAG 
GACCCGGCTA ACATTCAGTC GAAGCACCAG AAGCACCAGG CCTTCGAGGC GGAGCTGGCG 
GCTAACGCCG ATCGCATCCA GAGCGTGCTA GCCATGGGCG GAAACCTCAT CGATAAGAAG 
CAGTGCAGCG GGTCGGAGGA CGCGGTGCAG AAGCGGCTGA CGCAGATCGC CGATCAGTGG 
GAGTACCTGA CCCATAAGAC TACCGAGAAG TCGCTGAAGC TAAAGGAGGC GAATAAGCAG 
CGCACCTATA TCGCTGCTGT CAAGGATTTG GACTTCTGGT TGGGCGAGGT CGAGAGTCTT 
TTGACCACTG AGGATTCTGG TAAGGATTTG GCCTCTGTCC AAAACCTCAT GAAGAAGCAT 
CAGTTGGTCG AGGCGGATAT CGTGGCGCAC GAAGACCGCA TCAAGGACAT GAACAACCAG 
GCCGATTCCT TGGTGGAGAG CGGCCAGTTC GATACTGCCG GCATCCAGGA GAAGCGTCAA 
AGCATCAATG AGCGCTACGA GCGCATCTGC AACCTGGCGG CCCATCGCCA AGCCCGTCTC 
AACGAGGCTC TGACCCTGCA CCAATTCTTC CGCGACATCG CCGACGAGGA GAGCTGGATC 
AAGGAGAAGA AGCTACTTGT CGGCTCCGAC GACTATGGTC GCGATCTGAC CGGCGTCCAG 
AACCTCAAGA AGAAGCACAA GCGCCTAGAA GCCGAATTGG GCTCTCACGA GCCGGCCATT 
CAAGCTGTGC AGGAGGCTGG CGAGAAGCTC ATGGACGTGT CCAACCTCGG TGTCCCCGAA 
ATTGAGCAGC GCCTGAAGGC TCTGAACCAG GCCTGGGCCG AGCTAAAGAA CCTGGCTGCC 
ACGCGAGGTC AGAAGTTGGA TGAGTCGCTG ACCTATCAGC AATTCCTCGC TCAGGTGGAG 
GAGGAGGAGG CCTGGATCAC CGAGAAGCAA CAGCTGCTCT CTGTGGAGGA CTACGGCGAC 
TCGATGGCCG CCGTACAAGG ACTGCTGAAA AAGCACGACG CATTCGAAAC GGACTTCACT 
GCCCACAAGG ACCGCTGTTC TTTGATCTGC GACCAGGGCA GTGAGTTGGT GGAGGCCAAG 
AACCACCACG GAGAGTCCAT TGCCCAACGC TGCCAGCAGC TGCGCCTGAA ACTGGACAAC 
CTGTCCGCCC TGGCTGCCCG CCGCAAGGGT GCCCTGCTGG ACAACTCGGC CTATCTGCAG 
TTCATGTGGA AGGCCGATGT GGTCGAGAGC TGGATCGACG ACAAGGAGAA CTATGTGCGC 
TCCGATGAGT TCGGACGCGA CCTGTCCACC GTGCAAACGC TGTTGACCAA GCAAGAGACA 
TTCGATGCAG GTCTCAATGC CTTCGAGCAG GAGGGCATAC ACAACATCAC GGCACTTAAG 
GACCAACTGA TAAATGCCAG TCACGCCCAG TCGCCGGCAA TCCTAAAGCG TCACGGAGAC 
GTGATTGCCA GGTGGCAGAA GTTGCGCGAC GCCTCCAACA CGCGCAAGGA CCGACTGCTG 
GCCATGCAGG AGCAGTTCCG CCAGATCGAG GAACTCTACT TGACATTTGC CAAGAAAGCT 
TCGGCCTTCA ATTCTTGGTT CGAAAATGCC GAGGAGGATC TCACAGATCC TGTTCGCTGC 
AATTCGATCG AAGAAATCCG CGCCCTGCGC GACGCCCATG CCCAATTCCA GGCGTCCCTT 
TCGTCGGCCG AAGCTGACTT CAAGGCATTG GCAGCACTCG ACCAGAAGAT CAAGAGCTTT 
AATGTTGGAC CCAACCCGTA CACGTGGTTC ACCATGGAAG CTCTTGAGGA GACCTGGCGT 
AATCTGCAAA AGATCATAGA GGAACGCGAT GGGGAACTTG CCAAGGAGGC CAAGCGGCAG 
GAGGAGAACG ACAAGCTGCG CAAGGAGTTT GCCAAGCACG CCAATCTTTT CCACCAGTGG 
CTAACAGAGA CAAGAACGTC CATGATGGAA GGCTCCGGTT CCCTGGAACA GCAACTGGAG 
GCGCTTCGTG TCAAGGCCAC CGAGGTGCGC GCACGTCGCG TTGACCTCAA GAAGATCGAG 
GAGCTGGGAG CCTTGCTGGA GGAGCACCTT ATTCTGGACA ACCGCTACAC GGAACACTCG 
ACGGTGGGCC TGGCCCAGCA GTGGGATCAG CTTGACCAGC TGTCTATGCG CATGCAGCAC 
AACTTAGAGC AACAGATCCA GGCACGCAAC CACTCGGGCG TCTCCGAGGA CTCACTCAAG 
GAGTTCTCGA TGATGTTCAA GCACTTCGAT AAGGACAAGA GCGGCAAGCT CAACCACCAG 
GAATTCAAGT CCTGCCTGCG CGCCCTCGGC TACGACCTGC CCATGGTGGA GGAGGGACAG 
CCCGACCCGG AGTTCGAAGC AATTCTCGAT GTTGTCGACC CCAACCGCGA TGGCTACGTC 
TCTCTGCAGG AATACATCGC GTTCATGATC TCCAAGGAGA CGGAGAACGT TCAATCCTAC 
GAGGAGATCG AGAATGCCTT CCGCGCCATC ACCGCTGCCG ACCGCCCCTA TGTAACTAAG 
GAGGAGCTCT ACTGCAACCT CACCAAGGAC ATGGCTGACT ACTGCGTGCA GCGCATGAAA 
CCCTTCTCGG AACCGCGCTC CGGCCAGCCC ATCAAGGATG CCCTGGACTA CATAGACTTC 
ACGCGAACGC TGTTCCAGAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261287.1
CDS	277..7620
Translation	MENFTPKEVKILETVEDIQERREQVLSRYNDFKIETRQKREKLEDSRRFQYFKRDADELESWIHEKLQAASEESYRDPTNLQAKIQKHQAFEAEVSAHSNAIVSLDNTGQEMINQQHFASESIQVRLDELHKLWELLLSRLAEKGLKLQQALVLVQFLRQCEEVMFWIKDKETFVTADEFGQDLEHVEVLQRKFDEFQKDMASQEYRVTEVNQLADKLVQDGHPERDTITKRKEELNEAWQRLKQLAIVRQEKLFGAHEIQRFNRDADETVAWIAEKDVVLSSDDYGRDLASVQALQRKHEGVERDLAALEDKVSTLGAEAQRLCSIHADHSDQIRDKQAEIANYWQSLTTKARERKQKLDESYYLHRFLADFRDLVSWINGMKAIISADELAKDVAGAEALLERHQEHKGEIDAREDSFKLTTESGQKLLEREHYAAAEIQEKLAALENDKSSLLSLWEDRRILYEQCMDLQLFYRDTEQADTWMAKQEAFLANEDLGDSLDSVEALIKKHEDFEKSLAAQEEKIKALDIFATKLIDGQHYAADDVAQRRQMLLARRAALQEKSSKRRQLLEDSNRYQQFERDCDETKGWISEKLKFATDDSYLDPTNLNGKMQKHQNFEHELNANKSRIEDITNVGTELIEKQHYAADQINTRMQEIVVLWETLVQASDKKGTKLNEACQQQQFNRTIEDIELWLSEIEGQLLSEDHGKDLTSVQNLQKKHALLEADVMAHQDRIESIKVAANKFIESGHFDADNIRNKEGNLSARYAALAAPMGERKQHLLDSLQVQQLFRDLEDEAAWIREKEPIAASTNRGRDLIGVQNLIKKHQAVLAEINNHEARLLNVISSGENMLKDQPFASDDIRQRLEALQEQWNTLKEKSSQRKQDLDDSLQAHQYFADANEAESWMREKEPIATGSDYGKDEDSSEALLKKHEALVSDLEAFGNTIQALQEQAKNCRQQETPVVDITGKECVVALYDYTEKSPREVSMKKGDVLTLLNSNNKDWWKVEVNDRQGFVPAAYIKKIDAGLSASQQNLVDNHSIAKRQNQINSQYDNLLALARERQNKLNETVKAYVLVREAADLAQWIRDKENHAQIADVVGEDLEEVEVLQKKFDDFNDDLKANEVRLANMNEIAVQLTSLGQTEAALKIQTQMQDLNEKWNNLQTLTAEKASQLGSAHEVQRFHRDIDETKDWIAEKANALNNDDLGKDLRSVQTLQRKHEGVERDLAALRDKIRQLDETANRLMQSHPDTAEQTYAKQKEINEMWDQIITKSTARKEKLLDSYDLQRFLSDYRDLLAWINSMMSLVTSDELANDVTGAEALIERHQARRAEIGFTLGISSAPGAAASTSSIASPSGDEEHRTEIDARAGTFGAFEQFGNELLQANHYASPEIKEKIEDLAKAREDLEKAWTERRLQLEQNLDLQLYMRDCELAESWMSAREAFLNADDDANAGGNVEALIKKHEDFDKAINGHEQKIAALQTVADQLIAQNHYASNLVDEKRKQVLERWRHLKEGLIEKRSRLGDEQTLQQFSRDADEIENWIAEKLQLATEESYKDPANIQSKHQKHQAFEAELAANADRIQSVLAMGGNLIDKKQCSGSEDAVQKRLTQIADQWEYLTHKTTEKSLKLKEANKQRTYIAAVKDLDFWLGEVESLLTTEDSGKDLASVQNLMKKHQLVEADIVAHEDRIKDMNNQADSLVESGQFDTAGIQEKRQSINERYERICNLAAHRQARLNEALTLHQFFRDIADEESWIKEKKLLVGSDDYGRDLTGVQNLKKKHKRLEAELGSHEPAIQAVQEAGEKLMDVSNLGVPEIEQRLKALNQAWAELKNLAATRGQKLDESLTYQQFLAQVEEEEAWITEKQQLLSVEDYGDSMAAVQGLLKKHDAFETDFTAHKDRCSLICDQGSELVEAKNHHGESIAQRCQQLRLKLDNLSALAARRKGALLDNSAYLQFMWKADVVESWIDDKENYVRSDEFGRDLSTVQTLLTKQETFDAGLNAFEQEGIHNITALKDQLINASHAQSPAILKRHGDVIARWQKLRDASNTRKDRLLAMQEQFRQIEELYLTFAKKASAFNSWFENAEEDLTDPVRCNSIEEIRALRDAHAQFQASLSSAEADFKALAALDQKIKSFNVGPNPYTWFTMEALEETWRNLQKIIEERDGELAKEAKRQEENDKLRKEFAKHANLFHQWLTETRTSMMEGSGSLEQQLEALRVKATEVRARRVDLKKIEELGALLEEHLILDNRYTEHSTVGLAQQWDQLDQLSMRMQHNLEQQIQARNHSGVSEDSLKEFSMMFKHFDKDKSGKLNHQEFKSCLRALGYDLPMVEEGQPDPEFEAILDVVDPNRDGYVSLQEYIAFMISKETENVQSYEEIENAFRAITAADRPYVTKEELYCNLTKDMADYCVQRMKPFSEPRSGQPIKDALDYIDFTRTLFQN

Target ORF information:

RefSeq Version	NM_001274358.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alpha spectrin, transcript variant C (alpha-Spec), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274358.1

ORF Insert Sequence:

ATGGAGAACT TTACACCCAA AGAGGTGAAG ATCCTCGAGA CAGTCGAGGA CATCCAGGAG 
CGACGTGAGC AGGTTCTGTC CCGCTACAAT GACTTCAAGA TCGAGACGCG CCAGAAGCGT 
GAAAAGCTCG AGGACTCGCG CCGCTTCCAG TACTTCAAGC GCGATGCCGA CGAGTTGGAG 
TCGTGGATCC ACGAGAAGCT GCAGGCAGCC AGCGAGGAGA GCTACCGCGA TCCGACCAAC 
CTGCAGGCCA AAATCCAGAA ACATCAGGCG TTCGAGGCGG AGGTGTCGGC GCACAGCAAC 
GCCATTGTCT CGCTGGACAA CACTGGCCAG GAGATGATCA ACCAGCAACA TTTTGCCTCC 
GAGTCGATTC AGGTCCGCCT CGACGAGCTG CACAAGCTGT GGGAGCTGCT TCTTAGCCGT 
CTGGCCGAGA AGGGTCTAAA GCTGCAGCAG GCTCTCGTCC TGGTGCAGTT CCTGCGCCAG 
TGCGAGGAGG TGATGTTCTG GATTAAGGAC AAGGAGACCT TTGTCACCGC CGACGAGTTC 
GGCCAAGACC TGGAACATGT AGAGGTTCTG CAGCGCAAGT TCGATGAGTT CCAGAAGGAC 
ATGGCCTCCC AGGAGTATCG CGTGACTGAG GTCAACCAGT TGGCCGATAA GTTGGTGCAA 
GATGGTCATC CCGAGCGCGA CACTATTACC AAGCGCAAGG AGGAGCTAAA CGAGGCTTGG 
CAGCGACTTA AGCAGTTAGC CATTGTGCGC CAAGAGAAGC TATTCGGTGC CCACGAGATC 
CAGCGTTTCA ATCGCGATGC CGACGAAACC GTCGCCTGGA TTGCCGAGAA GGATGTGGTC 
CTGTCGTCCG ATGATTATGG CCGCGATTTG GCCAGTGTTC AGGCGCTGCA ACGCAAACAC 
GAAGGAGTGG AGCGGGATTT GGCCGCCTTG GAGGATAAGG TTTCCACACT GGGAGCCGAG 
GCCCAGCGGC TGTGCTCCAT TCACGCCGAT CACAGTGACC AGATCCGTGA CAAACAGGCC 
GAGATTGCCA ACTACTGGCA GAGTCTCACC ACCAAGGCTC GCGAGCGCAA GCAGAAGCTA 
GATGAGTCCT ACTACCTGCA CCGTTTCCTC GCCGATTTCC GCGACCTCGT GTCCTGGATC 
AATGGCATGA AGGCCATCAT CTCGGCCGAT GAGCTGGCTA AGGATGTGGC TGGAGCAGAG 
GCTCTTCTGG AACGTCACCA GGAGCACAAG GGCGAGATCG ACGCTCGTGA GGATAGCTTC 
AAGCTGACCA CTGAATCCGG ACAGAAGCTG CTGGAGCGTG AGCATTACGC CGCCGCCGAA 
ATCCAGGAAA AGCTTGCCGC TCTGGAAAAC GACAAGAGTT CACTGCTCTC GCTGTGGGAA 
GATCGTCGCA TCCTGTACGA GCAGTGCATG GATCTGCAAC TGTTTTACCG CGATACGGAG 
CAGGCTGACA CTTGGATGGC CAAACAGGAG GCCTTCCTGG CCAACGAGGA CCTTGGCGAC 
TCCCTGGACT CTGTGGAGGC GCTAATTAAG AAGCACGAGG ACTTCGAGAA GAGTCTGGCT 
GCCCAGGAGG AGAAGATCAA GGCTTTAGAC ATCTTTGCCA CCAAATTAAT CGATGGTCAG 
CATTATGCCG CCGATGATGT GGCCCAGCGC CGTCAGATGC TCCTAGCCCG GCGGGCTGCT 
CTCCAAGAAA AATCGTCCAA GCGTCGCCAA TTGCTGGAGG ATTCGAACCG CTACCAGCAG 
TTCGAGCGCG ATTGCGACGA GACCAAGGGC TGGATTAGCG AGAAGCTGAA GTTTGCCACC 
GATGACAGCT ATTTGGATCC TACCAACCTT AACGGAAAGA TGCAGAAGCA CCAGAACTTT 
GAGCACGAGC TGAATGCCAA CAAGTCGCGC ATCGAGGACA TCACCAACGT GGGCACCGAG 
CTGATCGAGA AGCAGCACTA TGCTGCCGAC CAGATCAACA CCCGCATGCA GGAGATCGTC 
GTCCTGTGGG AGACCCTCGT CCAGGCTTCC GATAAAAAGG GAACCAAACT CAACGAGGCA 
TGCCAACAGC AGCAGTTCAA CCGCACCATC GAGGACATTG AGCTGTGGCT TAGCGAGATC 
GAGGGTCAAT TGTTGTCGGA GGATCACGGC AAAGACCTGA CCTCCGTTCA GAATTTGCAG 
AAGAAGCACG CCCTGCTGGA GGCCGATGTG ATGGCTCACC AGGATCGCAT TGAAAGCATC 
AAAGTGGCCG CCAACAAGTT CATCGAATCC GGTCACTTTG ACGCGGACAA CATTCGCAAC 
AAGGAGGGCA ATCTGTCGGC TCGCTATGCC GCCCTAGCTG CACCCATGGG CGAGAGGAAA 
CAACACCTTC TTGACTCCCT TCAGGTGCAG CAGCTCTTCC GCGACTTGGA GGACGAGGCT 
GCCTGGATCC GTGAGAAGGA ACCCATTGCC GCATCCACGA ACCGCGGTCG TGACTTGATT 
GGTGTGCAAA ACCTGATCAA GAAACACCAG GCTGTGCTGG CCGAGATCAA CAACCATGAG 
GCCAGGCTGC TGAATGTGAT CAGTTCGGGC GAGAACATGC TGAAGGACCA GCCCTTCGCC 
AGCGACGATA TCCGTCAGCG TTTGGAGGCG CTCCAAGAGC AGTGGAACAC GCTGAAGGAG 
AAGTCCAGCC AGCGAAAGCA GGATCTGGAT GACTCTCTCC AGGCACACCA GTACTTTGCC 
GACGCCAACG AAGCTGAGTC GTGGATGCGC GAAAAGGAAC CGATTGCAAC TGGCAGTGAT 
TATGGTAAGG ACGAGGATTC CTCGGAGGCT TTGCTCAAGA AGCACGAGGC TCTGGTCTCC 
GATCTAGAGG CTTTTGGCAA CACCATTCAG GCGCTGCAGG AGCAGGCCAA GAACTGTCGC 
CAGCAGGAGA CCCCTGTTGT GGATATCACT GGCAAGGAGT GCGTGGTTGC CCTCTACGAC 
TACACGGAGA AATCCCCTCG TGAGGTCTCG ATGAAGAAGG GCGATGTTCT GACCCTGCTG 
AACTCGAACA ACAAAGACTG GTGGAAGGTG GAGGTCAACG ACCGCCAGGG CTTCGTACCG 
GCCGCATACA TCAAGAAGAT TGACGCCGGC TTGAGCGCCT CCCAGCAGAA CCTAGTGGAC 
AATCATTCGA TTGCCAAGCG CCAGAACCAG ATCAACTCGC AGTACGACAA CTTGCTGGCC 
CTCGCCCGCG AGCGCCAGAA CAAGCTAAAC GAGACTGTGA AGGCGTACGT TCTTGTTCGT 
GAGGCTGCCG ACTTGGCCCA GTGGATCCGG GACAAGGAGA ACCACGCTCA GATCGCTGAT 
GTTGTGGGAG AGGATCTGGA AGAGGTGGAG GTGCTCCAAA AGAAGTTCGA CGACTTCAAC 
GACGACCTGA AGGCCAACGA AGTGCGTCTG GCAAACATGA ACGAGATTGC CGTGCAGCTG 
ACTTCCTTGG GTCAGACCGA GGCCGCCCTA AAGATCCAAA CCCAGATGCA AGACCTGAAC 
GAGAAGTGGA ACAACCTGCA GACGCTGACC GCTGAGAAGG CCAGCCAACT GGGATCCGCC 
CATGAGGTGC AACGCTTCCA TCGTGACATT GACGAGACCA AGGATTGGAT CGCTGAGAAG 
GCCAATGCCC TGAACAACGA CGACCTGGGC AAGGATCTGC GCAGTGTCCA GACTCTGCAG 
CGCAAGCACG AGGGCGTGGA ACGCGACTTG GCCGCATTGC GGGACAAGAT CCGCCAGCTG 
GACGAAACCG CCAACAGGCT GATGCAGTCG CACCCGGACA CCGCCGAGCA GACCTACGCC 
AAGCAGAAGG AGATCAACGA GATGTGGGAC CAGATCATCA CCAAGTCCAC CGCCCGCAAG 
GAGAAGCTGC TGGACTCGTA CGACCTGCAG CGTTTCCTCA GCGACTACCG CGATCTGCTG 
GCCTGGATCA ACTCGATGAT GAGCCTGGTC ACGTCCGACG AGCTGGCCAA CGATGTAACA 
GGAGCCGAGG CTCTGATCGA GCGTCATCAG GCACGCCGTG CCGAGATTGG GTTCACATTG 
GGCATCAGCT CGGCTCCCGG AGCCGCCGCC TCGACCTCCA GCATCGCATC TCCATCCGGC 
GACGAGGAAC ACCGAACGGA GATCGATGCG CGCGCCGGAA CTTTCGGGGC TTTCGAGCAG 
TTCGGAAACG AGCTGCTGCA GGCCAACCAC TATGCGTCGC CTGAAATCAA GGAGAAAATC 
GAGGATCTGG CCAAGGCCCG CGAGGACTTG GAGAAGGCGT GGACAGAGCG CCGCCTGCAG 
CTGGAGCAGA ACTTGGATCT GCAGCTCTAC ATGCGTGACT GCGAGTTGGC CGAGTCCTGG 
ATGTCAGCCC GCGAGGCCTT CCTAAATGCC GATGATGATG CCAATGCCGG CGGAAATGTG 
GAGGCACTGA TCAAGAAGCA CGAGGACTTC GACAAGGCCA TTAACGGCCA CGAACAGAAG 
ATTGCTGCTC TGCAGACGGT GGCGGATCAG TTGATCGCCC AGAACCACTA TGCCTCCAAT 
TTGGTGGACG AGAAGCGCAA GCAAGTGCTG GAGCGCTGGC GCCATCTCAA GGAGGGTCTG 
ATCGAGAAGC GATCCCGTTT GGGTGACGAG CAGACGCTGC AGCAGTTCTC GCGCGATGCC 
GATGAGATTG AGAACTGGAT TGCAGAGAAG TTGCAGCTGG CCACCGAAGA GAGCTACAAG 
GACCCGGCTA ACATTCAGTC GAAGCACCAG AAGCACCAGG CCTTCGAGGC GGAGCTGGCG 
GCTAACGCCG ATCGCATCCA GAGCGTGCTA GCCATGGGCG GAAACCTCAT CGATAAGAAG 
CAGTGCAGCG GGTCGGAGGA CGCGGTGCAG AAGCGGCTGA CGCAGATCGC CGATCAGTGG 
GAGTACCTGA CCCATAAGAC TACCGAGAAG TCGCTGAAGC TAAAGGAGGC GAATAAGCAG 
CGCACCTATA TCGCTGCTGT CAAGGATTTG GACTTCTGGT TGGGCGAGGT CGAGAGTCTT 
TTGACCACTG AGGATTCTGG TAAGGATTTG GCCTCTGTCC AAAACCTCAT GAAGAAGCAT 
CAGTTGGTCG AGGCGGATAT CGTGGCGCAC GAAGACCGCA TCAAGGACAT GAACAACCAG 
GCCGATTCCT TGGTGGAGAG CGGCCAGTTC GATACTGCCG GCATCCAGGA GAAGCGTCAA 
AGCATCAATG AGCGCTACGA GCGCATCTGC AACCTGGCGG CCCATCGCCA AGCCCGTCTC 
AACGAGGCTC TGACCCTGCA CCAATTCTTC CGCGACATCG CCGACGAGGA GAGCTGGATC 
AAGGAGAAGA AGCTACTTGT CGGCTCCGAC GACTATGGTC GCGATCTGAC CGGCGTCCAG 
AACCTCAAGA AGAAGCACAA GCGCCTAGAA GCCGAATTGG GCTCTCACGA GCCGGCCATT 
CAAGCTGTGC AGGAGGCTGG CGAGAAGCTC ATGGACGTGT CCAACCTCGG TGTCCCCGAA 
ATTGAGCAGC GCCTGAAGGC TCTGAACCAG GCCTGGGCCG AGCTAAAGAA CCTGGCTGCC 
ACGCGAGGTC AGAAGTTGGA TGAGTCGCTG ACCTATCAGC AATTCCTCGC TCAGGTGGAG 
GAGGAGGAGG CCTGGATCAC CGAGAAGCAA CAGCTGCTCT CTGTGGAGGA CTACGGCGAC 
TCGATGGCCG CCGTACAAGG ACTGCTGAAA AAGCACGACG CATTCGAAAC GGACTTCACT 
GCCCACAAGG ACCGCTGTTC TTTGATCTGC GACCAGGGCA GTGAGTTGGT GGAGGCCAAG 
AACCACCACG GAGAGTCCAT TGCCCAACGC TGCCAGCAGC TGCGCCTGAA ACTGGACAAC 
CTGTCCGCCC TGGCTGCCCG CCGCAAGGGT GCCCTGCTGG ACAACTCGGC CTATCTGCAG 
TTCATGTGGA AGGCCGATGT GGTCGAGAGC TGGATCGACG ACAAGGAGAA CTATGTGCGC 
TCCGATGAGT TCGGACGCGA CCTGTCCACC GTGCAAACGC TGTTGACCAA GCAAGAGACA 
TTCGATGCAG GTCTCAATGC CTTCGAGCAG GAGGGCATAC ACAACATCAC GGCACTTAAG 
GACCAACTGA TAAATGCCAG TCACGCCCAG TCGCCGGCAA TCCTAAAGCG TCACGGAGAC 
GTGATTGCCA GGTGGCAGAA GTTGCGCGAC GCCTCCAACA CGCGCAAGGA CCGACTGCTG 
GCCATGCAGG AGCAGTTCCG CCAGATCGAG GAACTCTACT TGACATTTGC CAAGAAAGCT 
TCGGCCTTCA ATTCTTGGTT CGAAAATGCC GAGGAGGATC TCACAGATCC TGTTCGCTGC 
AATTCGATCG AAGAAATCCG CGCCCTGCGC GACGCCCATG CCCAATTCCA GGCGTCCCTT 
TCGTCGGCCG AAGCTGACTT CAAGGCATTG GCAGCACTCG ACCAGAAGAT CAAGAGCTTT 
AATGTTGGAC CCAACCCGTA CACGTGGTTC ACCATGGAAG CTCTTGAGGA GACCTGGCGT 
AATCTGCAAA AGATCATAGA GGAACGCGAT GGGGAACTTG CCAAGGAGGC CAAGCGGCAG 
GAGGAGAACG ACAAGCTGCG CAAGGAGTTT GCCAAGCACG CCAATCTTTT CCACCAGTGG 
CTAACAGAGA CAAGAACGTC CATGATGGAA GGCTCCGGTT CCCTGGAACA GCAACTGGAG 
GCGCTTCGTG TCAAGGCCAC CGAGGTGCGC GCACGTCGCG TTGACCTCAA GAAGATCGAG 
GAGCTGGGAG CCTTGCTGGA GGAGCACCTT ATTCTGGACA ACCGCTACAC GGAACACTCG 
ACGGTGGGCC TGGCCCAGCA GTGGGATCAG CTTGACCAGC TGTCTATGCG CATGCAGCAC 
AACTTAGAGC AACAGATCCA GGCACGCAAC CACTCGGGCG TCTCCGAGGA CTCACTCAAG 
GAGTTCTCGA TGATGTTCAA GCACTTCGAT AAGGACAAGA GCGGCAAGCT CAACCACCAG 
GAATTCAAGT CCTGCCTGCG CGCCCTCGGC TACGACCTGC CCATGGTGGA GGAGGGACAG 
CCCGACCCGG AGTTCGAAGC AATTCTCGAT GTTGTCGACC CCAACCGCGA TGGCTACGTC 
TCTCTGCAGG AATACATCGC GTTCATGATC TCCAAGGAGA CGGAGAACGT TCAATCCTAC 
GAGGAGATCG AGAATGCCTT CCGCGCCATC ACCGCTGCCG ACCGCCCCTA TGTAACTAAG 
GAGGAGCTCT ACTGCAACCT CACCAAGGAC ATGGCTGACT ACTGCGTGCA GCGCATGAAA 
CCCTTCTCGG AACCGCGCTC CGGCCAGCCC ATCAAGGATG CCCTGGACTA CATAGACTTC 
ACGCGAACGC TGTTCCAGAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

alpha-Spec ( NM_001274357.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261286.1 Drosophila melanogaster alpha spectrin, transcript variant B (alpha-Spec), mRNA.

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CloneID	OFa20439
Clone ID Related Accession (Same CDS sequence)	NM_001274357.1
Accession Version	NM_001274357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	alpha spectrin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAACT TTACACCCAA AGAGGTGAAG ATCCTCGAGA CAGTCGAGGA CATCCAGGAG 
CGACGTGAGC AGGTTCTGTC CCGCTACAAT GACTTCAAGA TCGAGACGCG CCAGAAGCGT 
GAAAAGCTCG AGGACTCGCG CCGCTTCCAG TACTTCAAGC GCGATGCCGA CGAGTTGGAG 
TCGTGGATCC ACGAGAAGCT GCAGGCAGCC AGCGAGGAGA GCTACCGCGA TCCGACCAAC 
CTGCAGGCCA AAATCCAGAA ACATCAGGCG TTCGAGGCGG AGGTGTCGGC GCACAGCAAC 
GCCATTGTCT CGCTGGACAA CACTGGCCAG GAGATGATCA ACCAGCAACA TTTTGCCTCC 
GAGTCGATTC AGGTCCGCCT CGACGAGCTG CACAAGCTGT GGGAGCTGCT TCTTAGCCGT 
CTGGCCGAGA AGGGTCTAAA GCTGCAGCAG GCTCTCGTCC TGGTGCAGTT CCTGCGCCAG 
TGCGAGGAGG TGATGTTCTG GATTAAGGAC AAGGAGACCT TTGTCACCGC CGACGAGTTC 
GGCCAAGACC TGGAACATGT AGAGGTTCTG CAGCGCAAGT TCGATGAGTT CCAGAAGGAC 
ATGGCCTCCC AGGAGTATCG CGTGACTGAG GTCAACCAGT TGGCCGATAA GTTGGTGCAA 
GATGGTCATC CCGAGCGCGA CACTATTACC AAGCGCAAGG AGGAGCTAAA CGAGGCTTGG 
CAGCGACTTA AGCAGTTAGC CATTGTGCGC CAAGAGAAGC TATTCGGTGC CCACGAGATC 
CAGCGTTTCA ATCGCGATGC CGACGAAACC GTCGCCTGGA TTGCCGAGAA GGATGTGGTC 
CTGTCGTCCG ATGATTATGG CCGCGATTTG GCCAGTGTTC AGGCGCTGCA ACGCAAACAC 
GAAGGAGTGG AGCGGGATTT GGCCGCCTTG GAGGATAAGG TTTCCACACT GGGAGCCGAG 
GCCCAGCGGC TGTGCTCCAT TCACGCCGAT CACAGTGACC AGATCCGTGA CAAACAGGCC 
GAGATTGCCA ACTACTGGCA GAGTCTCACC ACCAAGGCTC GCGAGCGCAA GCAGAAGCTA 
GATGAGTCCT ACTACCTGCA CCGTTTCCTC GCCGATTTCC GCGACCTCGT GTCCTGGATC 
AATGGCATGA AGGCCATCAT CTCGGCCGAT GAGCTGGCTA AGGATGTGGC TGGAGCAGAG 
GCTCTTCTGG AACGTCACCA GGAGCACAAG GGCGAGATCG ACGCTCGTGA GGATAGCTTC 
AAGCTGACCA CTGAATCCGG ACAGAAGCTG CTGGAGCGTG AGCATTACGC CGCCGCCGAA 
ATCCAGGAAA AGCTTGCCGC TCTGGAAAAC GACAAGAGTT CACTGCTCTC GCTGTGGGAA 
GATCGTCGCA TCCTGTACGA GCAGTGCATG GATCTGCAAC TGTTTTACCG CGATACGGAG 
CAGGCTGACA CTTGGATGGC CAAACAGGAG GCCTTCCTGG CCAACGAGGA CCTTGGCGAC 
TCCCTGGACT CTGTGGAGGC GCTAATTAAG AAGCACGAGG ACTTCGAGAA GAGTCTGGCT 
GCCCAGGAGG AGAAGATCAA GGCTTTAGAC ATCTTTGCCA CCAAATTAAT CGATGGTCAG 
CATTATGCCG CCGATGATGT GGCCCAGCGC CGTCAGATGC TCCTAGCCCG GCGGGCTGCT 
CTCCAAGAAA AATCGTCCAA GCGTCGCCAA TTGCTGGAGG ATTCGAACCG CTACCAGCAG 
TTCGAGCGCG ATTGCGACGA GACCAAGGGC TGGATTAGCG AGAAGCTGAA GTTTGCCACC 
GATGACAGCT ATTTGGATCC TACCAACCTT AACGGAAAGA TGCAGAAGCA CCAGAACTTT 
GAGCACGAGC TGAATGCCAA CAAGTCGCGC ATCGAGGACA TCACCAACGT GGGCACCGAG 
CTGATCGAGA AGCAGCACTA TGCTGCCGAC CAGATCAACA CCCGCATGCA GGAGATCGTC 
GTCCTGTGGG AGACCCTCGT CCAGGCTTCC GATAAAAAGG GAACCAAACT CAACGAGGCA 
TGCCAACAGC AGCAGTTCAA CCGCACCATC GAGGACATTG AGCTGTGGCT TAGCGAGATC 
GAGGGTCAAT TGTTGTCGGA GGATCACGGC AAAGACCTGA CCTCCGTTCA GAATTTGCAG 
AAGAAGCACG CCCTGCTGGA GGCCGATGTG ATGGCTCACC AGGATCGCAT TGAAAGCATC 
AAAGTGGCCG CCAACAAGTT CATCGAATCC GGTCACTTTG ACGCGGACAA CATTCGCAAC 
AAGGAGGGCA ATCTGTCGGC TCGCTATGCC GCCCTAGCTG CACCCATGGG CGAGAGGAAA 
CAACACCTTC TTGACTCCCT TCAGGTGCAG CAGCTCTTCC GCGACTTGGA GGACGAGGCT 
GCCTGGATCC GTGAGAAGGA ACCCATTGCC GCATCCACGA ACCGCGGTCG TGACTTGATT 
GGTGTGCAAA ACCTGATCAA GAAACACCAG GCTGTGCTGG CCGAGATCAA CAACCATGAG 
GCCAGGCTGC TGAATGTGAT CAGTTCGGGC GAGAACATGC TGAAGGACCA GCCCTTCGCC 
AGCGACGATA TCCGTCAGCG TTTGGAGGCG CTCCAAGAGC AGTGGAACAC GCTGAAGGAG 
AAGTCCAGCC AGCGAAAGCA GGATCTGGAT GACTCTCTCC AGGCACACCA GTACTTTGCC 
GACGCCAACG AAGCTGAGTC GTGGATGCGC GAAAAGGAAC CGATTGCAAC TGGCAGTGAT 
TATGGTAAGG ACGAGGATTC CTCGGAGGCT TTGCTCAAGA AGCACGAGGC TCTGGTCTCC 
GATCTAGAGG CTTTTGGCAA CACCATTCAG GCGCTGCAGG AGCAGGCCAA GAACTGTCGC 
CAGCAGGAGA CCCCTGTTGT GGATATCACT GGCAAGGAGT GCGTGGTTGC CCTCTACGAC 
TACACGGAGA AATCCCCTCG TGAGGTCTCG ATGAAGAAGG GCGATGTTCT GACCCTGCTG 
AACTCGAACA ACAAAGACTG GTGGAAGGTG GAGGTCAACG ACCGCCAGGG CTTCGTACCG 
GCCGCATACA TCAAGAAGAT TGACGCCGGC TTGAGCGCCT CCCAGCAGAA CCTAGTGGAC 
AATCATTCGA TTGCCAAGCG CCAGAACCAG ATCAACTCGC AGTACGACAA CTTGCTGGCC 
CTCGCCCGCG AGCGCCAGAA CAAGCTAAAC GAGACTGTGA AGGCGTACGT TCTTGTTCGT 
GAGGCTGCCG ACTTGGCCCA GTGGATCCGG GACAAGGAGA ACCACGCTCA GATCGCTGAT 
GTTGTGGGAG AGGATCTGGA AGAGGTGGAG GTGCTCCAAA AGAAGTTCGA CGACTTCAAC 
GACGACCTGA AGGCCAACGA AGTGCGTCTG GCAAACATGA ACGAGATTGC CGTGCAGCTG 
ACTTCCTTGG GTCAGACCGA GGCCGCCCTA AAGATCCAAA CCCAGATGCA AGACCTGAAC 
GAGAAGTGGA ACAACCTGCA GACGCTGACC GCTGAGAAGG CCAGCCAACT GGGATCCGCC 
CATGAGGTGC AACGCTTCCA TCGTGACATT GACGAGACCA AGGATTGGAT CGCTGAGAAG 
GCCAATGCCC TGAACAACGA CGACCTGGGC AAGGATCTGC GCAGTGTCCA GACTCTGCAG 
CGCAAGCACG AGGGCGTGGA ACGCGACTTG GCCGCATTGC GGGACAAGAT CCGCCAGCTG 
GACGAAACCG CCAACAGGCT GATGCAGTCG CACCCGGACA CCGCCGAGCA GACCTACGCC 
AAGCAGAAGG AGATCAACGA GATGTGGGAC CAGATCATCA CCAAGTCCAC CGCCCGCAAG 
GAGAAGCTGC TGGACTCGTA CGACCTGCAG CGTTTCCTCA GCGACTACCG CGATCTGCTG 
GCCTGGATCA ACTCGATGAT GAGCCTGGTC ACGTCCGACG AGCTGGCCAA CGATGTAACA 
GGAGCCGAGG CTCTGATCGA GCGTCATCAG GCACGCCGTG CCGAGATTGG GTTCACATTG 
GGCATCAGCT CGGCTCCCGG AGCCGCCGCC TCGACCTCCA GCATCGCATC TCCATCCGGC 
GACGAGGAAC ACCGAACGGA GATCGATGCG CGCGCCGGAA CTTTCGGGGC TTTCGAGCAG 
TTCGGAAACG AGCTGCTGCA GGCCAACCAC TATGCGTCGC CTGAAATCAA GGAGAAAATC 
GAGGATCTGG CCAAGGCCCG CGAGGACTTG GAGAAGGCGT GGACAGAGCG CCGCCTGCAG 
CTGGAGCAGA ACTTGGATCT GCAGCTCTAC ATGCGTGACT GCGAGTTGGC CGAGTCCTGG 
ATGTCAGCCC GCGAGGCCTT CCTAAATGCC GATGATGATG CCAATGCCGG CGGAAATGTG 
GAGGCACTGA TCAAGAAGCA CGAGGACTTC GACAAGGCCA TTAACGGCCA CGAACAGAAG 
ATTGCTGCTC TGCAGACGGT GGCGGATCAG TTGATCGCCC AGAACCACTA TGCCTCCAAT 
TTGGTGGACG AGAAGCGCAA GCAAGTGCTG GAGCGCTGGC GCCATCTCAA GGAGGGTCTG 
ATCGAGAAGC GATCCCGTTT GGGTGACGAG CAGACGCTGC AGCAGTTCTC GCGCGATGCC 
GATGAGATTG AGAACTGGAT TGCAGAGAAG TTGCAGCTGG CCACCGAAGA GAGCTACAAG 
GACCCGGCTA ACATTCAGTC GAAGCACCAG AAGCACCAGG CCTTCGAGGC GGAGCTGGCG 
GCTAACGCCG ATCGCATCCA GAGCGTGCTA GCCATGGGCG GAAACCTCAT CGATAAGAAG 
CAGTGCAGCG GGTCGGAGGA CGCGGTGCAG AAGCGGCTGA CGCAGATCGC CGATCAGTGG 
GAGTACCTGA CCCATAAGAC TACCGAGAAG TCGCTGAAGC TAAAGGAGGC GAATAAGCAG 
CGCACCTATA TCGCTGCTGT CAAGGATTTG GACTTCTGGT TGGGCGAGGT CGAGAGTCTT 
TTGACCACTG AGGATTCTGG TAAGGATTTG GCCTCTGTCC AAAACCTCAT GAAGAAGCAT 
CAGTTGGTCG AGGCGGATAT CGTGGCGCAC GAAGACCGCA TCAAGGACAT GAACAACCAG 
GCCGATTCCT TGGTGGAGAG CGGCCAGTTC GATACTGCCG GCATCCAGGA GAAGCGTCAA 
AGCATCAATG AGCGCTACGA GCGCATCTGC AACCTGGCGG CCCATCGCCA AGCCCGTCTC 
AACGAGGCTC TGACCCTGCA CCAATTCTTC CGCGACATCG CCGACGAGGA GAGCTGGATC 
AAGGAGAAGA AGCTACTTGT CGGCTCCGAC GACTATGGTC GCGATCTGAC CGGCGTCCAG 
AACCTCAAGA AGAAGCACAA GCGCCTAGAA GCCGAATTGG GCTCTCACGA GCCGGCCATT 
CAAGCTGTGC AGGAGGCTGG CGAGAAGCTC ATGGACGTGT CCAACCTCGG TGTCCCCGAA 
ATTGAGCAGC GCCTGAAGGC TCTGAACCAG GCCTGGGCCG AGCTAAAGAA CCTGGCTGCC 
ACGCGAGGTC AGAAGTTGGA TGAGTCGCTG ACCTATCAGC AATTCCTCGC TCAGGTGGAG 
GAGGAGGAGG CCTGGATCAC CGAGAAGCAA CAGCTGCTCT CTGTGGAGGA CTACGGCGAC 
TCGATGGCCG CCGTACAAGG ACTGCTGAAA AAGCACGACG CATTCGAAAC GGACTTCACT 
GCCCACAAGG ACCGCTGTTC TTTGATCTGC GACCAGGGCA GTGAGTTGGT GGAGGCCAAG 
AACCACCACG GAGAGTCCAT TGCCCAACGC TGCCAGCAGC TGCGCCTGAA ACTGGACAAC 
CTGTCCGCCC TGGCTGCCCG CCGCAAGGGT GCCCTGCTGG ACAACTCGGC CTATCTGCAG 
TTCATGTGGA AGGCCGATGT GGTCGAGAGC TGGATCGACG ACAAGGAGAA CTATGTGCGC 
TCCGATGAGT TCGGACGCGA CCTGTCCACC GTGCAAACGC TGTTGACCAA GCAAGAGACA 
TTCGATGCAG GTCTCAATGC CTTCGAGCAG GAGGGCATAC ACAACATCAC GGCACTTAAG 
GACCAACTGA TAAATGCCAG TCACGCCCAG TCGCCGGCAA TCCTAAAGCG TCACGGAGAC 
GTGATTGCCA GGTGGCAGAA GTTGCGCGAC GCCTCCAACA CGCGCAAGGA CCGACTGCTG 
GCCATGCAGG AGCAGTTCCG CCAGATCGAG GAACTCTACT TGACATTTGC CAAGAAAGCT 
TCGGCCTTCA ATTCTTGGTT CGAAAATGCC GAGGAGGATC TCACAGATCC TGTTCGCTGC 
AATTCGATCG AAGAAATCCG CGCCCTGCGC GACGCCCATG CCCAATTCCA GGCGTCCCTT 
TCGTCGGCCG AAGCTGACTT CAAGGCATTG GCAGCACTCG ACCAGAAGAT CAAGAGCTTT 
AATGTTGGAC CCAACCCGTA CACGTGGTTC ACCATGGAAG CTCTTGAGGA GACCTGGCGT 
AATCTGCAAA AGATCATAGA GGAACGCGAT GGGGAACTTG CCAAGGAGGC CAAGCGGCAG 
GAGGAGAACG ACAAGCTGCG CAAGGAGTTT GCCAAGCACG CCAATCTTTT CCACCAGTGG 
CTAACAGAGA CAAGATATTA TATGCTGGGT TATAACCGTC AAGGAACGTC CATGATGGAA 
GGCTCCGGTT CCCTGGAACA GCAACTGGAG GCGCTTCGTG TCAAGGCCAC CGAGGTGCGC 
GCACGTCGCG TTGACCTCAA GAAGATCGAG GAGCTGGGAG CCTTGCTGGA GGAGCACCTT 
ATTCTGGACA ACCGCTACAC GGAACACTCG ACGGTGGGCC TGGCCCAGCA GTGGGATCAG 
CTTGACCAGC TGTCTATGCG CATGCAGCAC AACTTAGAGC AACAGATCCA GGCACGCAAC 
CACTCGGGCG TCTCCGAGGA CTCACTCAAG GAGTTCTCGA TGATGTTCAA GCACTTCGAT 
AAGGACAAGA GCGGCAAGCT CAACCACCAG GAATTCAAGT CCTGCCTGCG CGCCCTCGGC 
TACGACCTGC CCATGGTGGA GGAGGGACAG CCCGACCCGG AGTTCGAAGC AATTCTCGAT 
GTTGTCGACC CCAACCGCGA TGGCTACGTC TCTCTGCAGG AATACATCGC GTTCATGATC 
TCCAAGGAGA CGGAGAACGT TCAATCCTAC GAGGAGATCG AGAATGCCTT CCGCGCCATC 
ACCGCTGCCG ACCGCCCCTA TGTAACTAAG GAGGAGCTCT ACTGCAACCT CACCAAGGAC 
ATGGCTGACT ACTGCGTGCA GCGCATGAAA CCCTTCTCGG AACCGCGCTC CGGCCAGCCC 
ATCAAGGATG CCCTGGACTA CATAGACTTC ACGCGAACGC TGTTCCAGAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261286.1
CDS	277..7650
Translation	MENFTPKEVKILETVEDIQERREQVLSRYNDFKIETRQKREKLEDSRRFQYFKRDADELESWIHEKLQAASEESYRDPTNLQAKIQKHQAFEAEVSAHSNAIVSLDNTGQEMINQQHFASESIQVRLDELHKLWELLLSRLAEKGLKLQQALVLVQFLRQCEEVMFWIKDKETFVTADEFGQDLEHVEVLQRKFDEFQKDMASQEYRVTEVNQLADKLVQDGHPERDTITKRKEELNEAWQRLKQLAIVRQEKLFGAHEIQRFNRDADETVAWIAEKDVVLSSDDYGRDLASVQALQRKHEGVERDLAALEDKVSTLGAEAQRLCSIHADHSDQIRDKQAEIANYWQSLTTKARERKQKLDESYYLHRFLADFRDLVSWINGMKAIISADELAKDVAGAEALLERHQEHKGEIDAREDSFKLTTESGQKLLEREHYAAAEIQEKLAALENDKSSLLSLWEDRRILYEQCMDLQLFYRDTEQADTWMAKQEAFLANEDLGDSLDSVEALIKKHEDFEKSLAAQEEKIKALDIFATKLIDGQHYAADDVAQRRQMLLARRAALQEKSSKRRQLLEDSNRYQQFERDCDETKGWISEKLKFATDDSYLDPTNLNGKMQKHQNFEHELNANKSRIEDITNVGTELIEKQHYAADQINTRMQEIVVLWETLVQASDKKGTKLNEACQQQQFNRTIEDIELWLSEIEGQLLSEDHGKDLTSVQNLQKKHALLEADVMAHQDRIESIKVAANKFIESGHFDADNIRNKEGNLSARYAALAAPMGERKQHLLDSLQVQQLFRDLEDEAAWIREKEPIAASTNRGRDLIGVQNLIKKHQAVLAEINNHEARLLNVISSGENMLKDQPFASDDIRQRLEALQEQWNTLKEKSSQRKQDLDDSLQAHQYFADANEAESWMREKEPIATGSDYGKDEDSSEALLKKHEALVSDLEAFGNTIQALQEQAKNCRQQETPVVDITGKECVVALYDYTEKSPREVSMKKGDVLTLLNSNNKDWWKVEVNDRQGFVPAAYIKKIDAGLSASQQNLVDNHSIAKRQNQINSQYDNLLALARERQNKLNETVKAYVLVREAADLAQWIRDKENHAQIADVVGEDLEEVEVLQKKFDDFNDDLKANEVRLANMNEIAVQLTSLGQTEAALKIQTQMQDLNEKWNNLQTLTAEKASQLGSAHEVQRFHRDIDETKDWIAEKANALNNDDLGKDLRSVQTLQRKHEGVERDLAALRDKIRQLDETANRLMQSHPDTAEQTYAKQKEINEMWDQIITKSTARKEKLLDSYDLQRFLSDYRDLLAWINSMMSLVTSDELANDVTGAEALIERHQARRAEIGFTLGISSAPGAAASTSSIASPSGDEEHRTEIDARAGTFGAFEQFGNELLQANHYASPEIKEKIEDLAKAREDLEKAWTERRLQLEQNLDLQLYMRDCELAESWMSAREAFLNADDDANAGGNVEALIKKHEDFDKAINGHEQKIAALQTVADQLIAQNHYASNLVDEKRKQVLERWRHLKEGLIEKRSRLGDEQTLQQFSRDADEIENWIAEKLQLATEESYKDPANIQSKHQKHQAFEAELAANADRIQSVLAMGGNLIDKKQCSGSEDAVQKRLTQIADQWEYLTHKTTEKSLKLKEANKQRTYIAAVKDLDFWLGEVESLLTTEDSGKDLASVQNLMKKHQLVEADIVAHEDRIKDMNNQADSLVESGQFDTAGIQEKRQSINERYERICNLAAHRQARLNEALTLHQFFRDIADEESWIKEKKLLVGSDDYGRDLTGVQNLKKKHKRLEAELGSHEPAIQAVQEAGEKLMDVSNLGVPEIEQRLKALNQAWAELKNLAATRGQKLDESLTYQQFLAQVEEEEAWITEKQQLLSVEDYGDSMAAVQGLLKKHDAFETDFTAHKDRCSLICDQGSELVEAKNHHGESIAQRCQQLRLKLDNLSALAARRKGALLDNSAYLQFMWKADVVESWIDDKENYVRSDEFGRDLSTVQTLLTKQETFDAGLNAFEQEGIHNITALKDQLINASHAQSPAILKRHGDVIARWQKLRDASNTRKDRLLAMQEQFRQIEELYLTFAKKASAFNSWFENAEEDLTDPVRCNSIEEIRALRDAHAQFQASLSSAEADFKALAALDQKIKSFNVGPNPYTWFTMEALEETWRNLQKIIEERDGELAKEAKRQEENDKLRKEFAKHANLFHQWLTETRYYMLGYNRQGTSMMEGSGSLEQQLEALRVKATEVRARRVDLKKIEELGALLEEHLILDNRYTEHSTVGLAQQWDQLDQLSMRMQHNLEQQIQARNHSGVSEDSLKEFSMMFKHFDKDKSGKLNHQEFKSCLRALGYDLPMVEEGQPDPEFEAILDVVDPNRDGYVSLQEYIAFMISKETENVQSYEEIENAFRAITAADRPYVTKEELYCNLTKDMADYCVQRMKPFSEPRSGQPIKDALDYIDFTRTLFQN

Target ORF information:

RefSeq Version	NM_001274357.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alpha spectrin, transcript variant B (alpha-Spec), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274357.1

ORF Insert Sequence:

ATGGAGAACT TTACACCCAA AGAGGTGAAG ATCCTCGAGA CAGTCGAGGA CATCCAGGAG 
CGACGTGAGC AGGTTCTGTC CCGCTACAAT GACTTCAAGA TCGAGACGCG CCAGAAGCGT 
GAAAAGCTCG AGGACTCGCG CCGCTTCCAG TACTTCAAGC GCGATGCCGA CGAGTTGGAG 
TCGTGGATCC ACGAGAAGCT GCAGGCAGCC AGCGAGGAGA GCTACCGCGA TCCGACCAAC 
CTGCAGGCCA AAATCCAGAA ACATCAGGCG TTCGAGGCGG AGGTGTCGGC GCACAGCAAC 
GCCATTGTCT CGCTGGACAA CACTGGCCAG GAGATGATCA ACCAGCAACA TTTTGCCTCC 
GAGTCGATTC AGGTCCGCCT CGACGAGCTG CACAAGCTGT GGGAGCTGCT TCTTAGCCGT 
CTGGCCGAGA AGGGTCTAAA GCTGCAGCAG GCTCTCGTCC TGGTGCAGTT CCTGCGCCAG 
TGCGAGGAGG TGATGTTCTG GATTAAGGAC AAGGAGACCT TTGTCACCGC CGACGAGTTC 
GGCCAAGACC TGGAACATGT AGAGGTTCTG CAGCGCAAGT TCGATGAGTT CCAGAAGGAC 
ATGGCCTCCC AGGAGTATCG CGTGACTGAG GTCAACCAGT TGGCCGATAA GTTGGTGCAA 
GATGGTCATC CCGAGCGCGA CACTATTACC AAGCGCAAGG AGGAGCTAAA CGAGGCTTGG 
CAGCGACTTA AGCAGTTAGC CATTGTGCGC CAAGAGAAGC TATTCGGTGC CCACGAGATC 
CAGCGTTTCA ATCGCGATGC CGACGAAACC GTCGCCTGGA TTGCCGAGAA GGATGTGGTC 
CTGTCGTCCG ATGATTATGG CCGCGATTTG GCCAGTGTTC AGGCGCTGCA ACGCAAACAC 
GAAGGAGTGG AGCGGGATTT GGCCGCCTTG GAGGATAAGG TTTCCACACT GGGAGCCGAG 
GCCCAGCGGC TGTGCTCCAT TCACGCCGAT CACAGTGACC AGATCCGTGA CAAACAGGCC 
GAGATTGCCA ACTACTGGCA GAGTCTCACC ACCAAGGCTC GCGAGCGCAA GCAGAAGCTA 
GATGAGTCCT ACTACCTGCA CCGTTTCCTC GCCGATTTCC GCGACCTCGT GTCCTGGATC 
AATGGCATGA AGGCCATCAT CTCGGCCGAT GAGCTGGCTA AGGATGTGGC TGGAGCAGAG 
GCTCTTCTGG AACGTCACCA GGAGCACAAG GGCGAGATCG ACGCTCGTGA GGATAGCTTC 
AAGCTGACCA CTGAATCCGG ACAGAAGCTG CTGGAGCGTG AGCATTACGC CGCCGCCGAA 
ATCCAGGAAA AGCTTGCCGC TCTGGAAAAC GACAAGAGTT CACTGCTCTC GCTGTGGGAA 
GATCGTCGCA TCCTGTACGA GCAGTGCATG GATCTGCAAC TGTTTTACCG CGATACGGAG 
CAGGCTGACA CTTGGATGGC CAAACAGGAG GCCTTCCTGG CCAACGAGGA CCTTGGCGAC 
TCCCTGGACT CTGTGGAGGC GCTAATTAAG AAGCACGAGG ACTTCGAGAA GAGTCTGGCT 
GCCCAGGAGG AGAAGATCAA GGCTTTAGAC ATCTTTGCCA CCAAATTAAT CGATGGTCAG 
CATTATGCCG CCGATGATGT GGCCCAGCGC CGTCAGATGC TCCTAGCCCG GCGGGCTGCT 
CTCCAAGAAA AATCGTCCAA GCGTCGCCAA TTGCTGGAGG ATTCGAACCG CTACCAGCAG 
TTCGAGCGCG ATTGCGACGA GACCAAGGGC TGGATTAGCG AGAAGCTGAA GTTTGCCACC 
GATGACAGCT ATTTGGATCC TACCAACCTT AACGGAAAGA TGCAGAAGCA CCAGAACTTT 
GAGCACGAGC TGAATGCCAA CAAGTCGCGC ATCGAGGACA TCACCAACGT GGGCACCGAG 
CTGATCGAGA AGCAGCACTA TGCTGCCGAC CAGATCAACA CCCGCATGCA GGAGATCGTC 
GTCCTGTGGG AGACCCTCGT CCAGGCTTCC GATAAAAAGG GAACCAAACT CAACGAGGCA 
TGCCAACAGC AGCAGTTCAA CCGCACCATC GAGGACATTG AGCTGTGGCT TAGCGAGATC 
GAGGGTCAAT TGTTGTCGGA GGATCACGGC AAAGACCTGA CCTCCGTTCA GAATTTGCAG 
AAGAAGCACG CCCTGCTGGA GGCCGATGTG ATGGCTCACC AGGATCGCAT TGAAAGCATC 
AAAGTGGCCG CCAACAAGTT CATCGAATCC GGTCACTTTG ACGCGGACAA CATTCGCAAC 
AAGGAGGGCA ATCTGTCGGC TCGCTATGCC GCCCTAGCTG CACCCATGGG CGAGAGGAAA 
CAACACCTTC TTGACTCCCT TCAGGTGCAG CAGCTCTTCC GCGACTTGGA GGACGAGGCT 
GCCTGGATCC GTGAGAAGGA ACCCATTGCC GCATCCACGA ACCGCGGTCG TGACTTGATT 
GGTGTGCAAA ACCTGATCAA GAAACACCAG GCTGTGCTGG CCGAGATCAA CAACCATGAG 
GCCAGGCTGC TGAATGTGAT CAGTTCGGGC GAGAACATGC TGAAGGACCA GCCCTTCGCC 
AGCGACGATA TCCGTCAGCG TTTGGAGGCG CTCCAAGAGC AGTGGAACAC GCTGAAGGAG 
AAGTCCAGCC AGCGAAAGCA GGATCTGGAT GACTCTCTCC AGGCACACCA GTACTTTGCC 
GACGCCAACG AAGCTGAGTC GTGGATGCGC GAAAAGGAAC CGATTGCAAC TGGCAGTGAT 
TATGGTAAGG ACGAGGATTC CTCGGAGGCT TTGCTCAAGA AGCACGAGGC TCTGGTCTCC 
GATCTAGAGG CTTTTGGCAA CACCATTCAG GCGCTGCAGG AGCAGGCCAA GAACTGTCGC 
CAGCAGGAGA CCCCTGTTGT GGATATCACT GGCAAGGAGT GCGTGGTTGC CCTCTACGAC 
TACACGGAGA AATCCCCTCG TGAGGTCTCG ATGAAGAAGG GCGATGTTCT GACCCTGCTG 
AACTCGAACA ACAAAGACTG GTGGAAGGTG GAGGTCAACG ACCGCCAGGG CTTCGTACCG 
GCCGCATACA TCAAGAAGAT TGACGCCGGC TTGAGCGCCT CCCAGCAGAA CCTAGTGGAC 
AATCATTCGA TTGCCAAGCG CCAGAACCAG ATCAACTCGC AGTACGACAA CTTGCTGGCC 
CTCGCCCGCG AGCGCCAGAA CAAGCTAAAC GAGACTGTGA AGGCGTACGT TCTTGTTCGT 
GAGGCTGCCG ACTTGGCCCA GTGGATCCGG GACAAGGAGA ACCACGCTCA GATCGCTGAT 
GTTGTGGGAG AGGATCTGGA AGAGGTGGAG GTGCTCCAAA AGAAGTTCGA CGACTTCAAC 
GACGACCTGA AGGCCAACGA AGTGCGTCTG GCAAACATGA ACGAGATTGC CGTGCAGCTG 
ACTTCCTTGG GTCAGACCGA GGCCGCCCTA AAGATCCAAA CCCAGATGCA AGACCTGAAC 
GAGAAGTGGA ACAACCTGCA GACGCTGACC GCTGAGAAGG CCAGCCAACT GGGATCCGCC 
CATGAGGTGC AACGCTTCCA TCGTGACATT GACGAGACCA AGGATTGGAT CGCTGAGAAG 
GCCAATGCCC TGAACAACGA CGACCTGGGC AAGGATCTGC GCAGTGTCCA GACTCTGCAG 
CGCAAGCACG AGGGCGTGGA ACGCGACTTG GCCGCATTGC GGGACAAGAT CCGCCAGCTG 
GACGAAACCG CCAACAGGCT GATGCAGTCG CACCCGGACA CCGCCGAGCA GACCTACGCC 
AAGCAGAAGG AGATCAACGA GATGTGGGAC CAGATCATCA CCAAGTCCAC CGCCCGCAAG 
GAGAAGCTGC TGGACTCGTA CGACCTGCAG CGTTTCCTCA GCGACTACCG CGATCTGCTG 
GCCTGGATCA ACTCGATGAT GAGCCTGGTC ACGTCCGACG AGCTGGCCAA CGATGTAACA 
GGAGCCGAGG CTCTGATCGA GCGTCATCAG GCACGCCGTG CCGAGATTGG GTTCACATTG 
GGCATCAGCT CGGCTCCCGG AGCCGCCGCC TCGACCTCCA GCATCGCATC TCCATCCGGC 
GACGAGGAAC ACCGAACGGA GATCGATGCG CGCGCCGGAA CTTTCGGGGC TTTCGAGCAG 
TTCGGAAACG AGCTGCTGCA GGCCAACCAC TATGCGTCGC CTGAAATCAA GGAGAAAATC 
GAGGATCTGG CCAAGGCCCG CGAGGACTTG GAGAAGGCGT GGACAGAGCG CCGCCTGCAG 
CTGGAGCAGA ACTTGGATCT GCAGCTCTAC ATGCGTGACT GCGAGTTGGC CGAGTCCTGG 
ATGTCAGCCC GCGAGGCCTT CCTAAATGCC GATGATGATG CCAATGCCGG CGGAAATGTG 
GAGGCACTGA TCAAGAAGCA CGAGGACTTC GACAAGGCCA TTAACGGCCA CGAACAGAAG 
ATTGCTGCTC TGCAGACGGT GGCGGATCAG TTGATCGCCC AGAACCACTA TGCCTCCAAT 
TTGGTGGACG AGAAGCGCAA GCAAGTGCTG GAGCGCTGGC GCCATCTCAA GGAGGGTCTG 
ATCGAGAAGC GATCCCGTTT GGGTGACGAG CAGACGCTGC AGCAGTTCTC GCGCGATGCC 
GATGAGATTG AGAACTGGAT TGCAGAGAAG TTGCAGCTGG CCACCGAAGA GAGCTACAAG 
GACCCGGCTA ACATTCAGTC GAAGCACCAG AAGCACCAGG CCTTCGAGGC GGAGCTGGCG 
GCTAACGCCG ATCGCATCCA GAGCGTGCTA GCCATGGGCG GAAACCTCAT CGATAAGAAG 
CAGTGCAGCG GGTCGGAGGA CGCGGTGCAG AAGCGGCTGA CGCAGATCGC CGATCAGTGG 
GAGTACCTGA CCCATAAGAC TACCGAGAAG TCGCTGAAGC TAAAGGAGGC GAATAAGCAG 
CGCACCTATA TCGCTGCTGT CAAGGATTTG GACTTCTGGT TGGGCGAGGT CGAGAGTCTT 
TTGACCACTG AGGATTCTGG TAAGGATTTG GCCTCTGTCC AAAACCTCAT GAAGAAGCAT 
CAGTTGGTCG AGGCGGATAT CGTGGCGCAC GAAGACCGCA TCAAGGACAT GAACAACCAG 
GCCGATTCCT TGGTGGAGAG CGGCCAGTTC GATACTGCCG GCATCCAGGA GAAGCGTCAA 
AGCATCAATG AGCGCTACGA GCGCATCTGC AACCTGGCGG CCCATCGCCA AGCCCGTCTC 
AACGAGGCTC TGACCCTGCA CCAATTCTTC CGCGACATCG CCGACGAGGA GAGCTGGATC 
AAGGAGAAGA AGCTACTTGT CGGCTCCGAC GACTATGGTC GCGATCTGAC CGGCGTCCAG 
AACCTCAAGA AGAAGCACAA GCGCCTAGAA GCCGAATTGG GCTCTCACGA GCCGGCCATT 
CAAGCTGTGC AGGAGGCTGG CGAGAAGCTC ATGGACGTGT CCAACCTCGG TGTCCCCGAA 
ATTGAGCAGC GCCTGAAGGC TCTGAACCAG GCCTGGGCCG AGCTAAAGAA CCTGGCTGCC 
ACGCGAGGTC AGAAGTTGGA TGAGTCGCTG ACCTATCAGC AATTCCTCGC TCAGGTGGAG 
GAGGAGGAGG CCTGGATCAC CGAGAAGCAA CAGCTGCTCT CTGTGGAGGA CTACGGCGAC 
TCGATGGCCG CCGTACAAGG ACTGCTGAAA AAGCACGACG CATTCGAAAC GGACTTCACT 
GCCCACAAGG ACCGCTGTTC TTTGATCTGC GACCAGGGCA GTGAGTTGGT GGAGGCCAAG 
AACCACCACG GAGAGTCCAT TGCCCAACGC TGCCAGCAGC TGCGCCTGAA ACTGGACAAC 
CTGTCCGCCC TGGCTGCCCG CCGCAAGGGT GCCCTGCTGG ACAACTCGGC CTATCTGCAG 
TTCATGTGGA AGGCCGATGT GGTCGAGAGC TGGATCGACG ACAAGGAGAA CTATGTGCGC 
TCCGATGAGT TCGGACGCGA CCTGTCCACC GTGCAAACGC TGTTGACCAA GCAAGAGACA 
TTCGATGCAG GTCTCAATGC CTTCGAGCAG GAGGGCATAC ACAACATCAC GGCACTTAAG 
GACCAACTGA TAAATGCCAG TCACGCCCAG TCGCCGGCAA TCCTAAAGCG TCACGGAGAC 
GTGATTGCCA GGTGGCAGAA GTTGCGCGAC GCCTCCAACA CGCGCAAGGA CCGACTGCTG 
GCCATGCAGG AGCAGTTCCG CCAGATCGAG GAACTCTACT TGACATTTGC CAAGAAAGCT 
TCGGCCTTCA ATTCTTGGTT CGAAAATGCC GAGGAGGATC TCACAGATCC TGTTCGCTGC 
AATTCGATCG AAGAAATCCG CGCCCTGCGC GACGCCCATG CCCAATTCCA GGCGTCCCTT 
TCGTCGGCCG AAGCTGACTT CAAGGCATTG GCAGCACTCG ACCAGAAGAT CAAGAGCTTT 
AATGTTGGAC CCAACCCGTA CACGTGGTTC ACCATGGAAG CTCTTGAGGA GACCTGGCGT 
AATCTGCAAA AGATCATAGA GGAACGCGAT GGGGAACTTG CCAAGGAGGC CAAGCGGCAG 
GAGGAGAACG ACAAGCTGCG CAAGGAGTTT GCCAAGCACG CCAATCTTTT CCACCAGTGG 
CTAACAGAGA CAAGATATTA TATGCTGGGT TATAACCGTC AAGGAACGTC CATGATGGAA 
GGCTCCGGTT CCCTGGAACA GCAACTGGAG GCGCTTCGTG TCAAGGCCAC CGAGGTGCGC 
GCACGTCGCG TTGACCTCAA GAAGATCGAG GAGCTGGGAG CCTTGCTGGA GGAGCACCTT 
ATTCTGGACA ACCGCTACAC GGAACACTCG ACGGTGGGCC TGGCCCAGCA GTGGGATCAG 
CTTGACCAGC TGTCTATGCG CATGCAGCAC AACTTAGAGC AACAGATCCA GGCACGCAAC 
CACTCGGGCG TCTCCGAGGA CTCACTCAAG GAGTTCTCGA TGATGTTCAA GCACTTCGAT 
AAGGACAAGA GCGGCAAGCT CAACCACCAG GAATTCAAGT CCTGCCTGCG CGCCCTCGGC 
TACGACCTGC CCATGGTGGA GGAGGGACAG CCCGACCCGG AGTTCGAAGC AATTCTCGAT 
GTTGTCGACC CCAACCGCGA TGGCTACGTC TCTCTGCAGG AATACATCGC GTTCATGATC 
TCCAAGGAGA CGGAGAACGT TCAATCCTAC GAGGAGATCG AGAATGCCTT CCGCGCCATC 
ACCGCTGCCG ACCGCCCCTA TGTAACTAAG GAGGAGCTCT ACTGCAACCT CACCAAGGAC 
ATGGCTGACT ACTGCGTGCA GCGCATGAAA CCCTTCTCGG AACCGCGCTC CGGCCAGCCC 
ATCAAGGATG CCCTGGACTA CATAGACTTC ACGCGAACGC TGTTCCAGAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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alpha-Spec cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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