mth cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mth
Entrez Gene ID	38058
Full Name	methuselah
Synonyms	CG6936,Dmel\CG6936,MTH,Mth
General protein information	Preferred Names methuselah Names CG6936 gene product from transcript CG6936-RB CG6936-PA CG6936-PB CG6936-PE methusalah mth-PA mth-PB mth-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61C1-61C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_079147.4	NP_523871.1	methuselah, isoform A
NM_167829.3	NP_728521.1	methuselah, isoform B
NM_001259606.2	NP_001246535.1	methuselah, isoform E

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	mthl4	NP_788394.1
Drosophila melanogaster (fruit fly)	mthl7	NP_788475.1
Drosophila melanogaster (fruit fly)	mthl11	NP_731479.3
Drosophila melanogaster (fruit fly)	mth	NP_728521.1
Drosophila melanogaster (fruit fly)	mthl3	NP_663308.1
Drosophila melanogaster (fruit fly)	mthl13	NP_788325.2
Drosophila melanogaster (fruit fly)	mthl10	NP_001246534.1
Drosophila melanogaster (fruit fly)	mthl2	NP_788462.2
Drosophila melanogaster (fruit fly)	mthl6	NP_788473.1
Drosophila melanogaster (fruit fly)	mthl12	NP_731791.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mth gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mth cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17065	NM_167829.3 Latest version!	Drosophila melanogaster methuselah (mth), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa17066	NM_079147.4 Latest version!	Drosophila melanogaster methuselah (mth), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa17066	NM_001259606.2 Latest version!	Drosophila melanogaster methuselah (mth), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mth ( NM_167829.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728521.1 Drosophila melanogaster methuselah (mth), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17065
Clone ID Related Accession (Same CDS sequence)	NM_167829.3
Accession Version	NM_167829.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	methuselah, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_167829.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAACTC TTTTGGTTCT TCGGATATCA ACTGTCATAC TTGTTGTGCT GGTTATTCAA 
AAATCATATG CAGACATTCT CGAATGCGAT TATTTCGACA CTGTCGATAT TTCGGCAGCT 
CAAAAACTAC AGAATGGATC GTACTTATTT GAGGGGTTGC TCGTTCCGGC CATTTTGACG 
GGAGAATATG ACTTTAGGAT TCTCCCGGAC GACTCGAAGC AGAAGGTGGC TCGTCACATA 
AGAGGATGTG TGTGCAAGCT GAAGCCCTGT GTCAGATTTT GCTGCCCTCA CGACCATATC 
ATGGATAATG GTGTCTGCTA CGACAACATG TCCGACGAGG AGCTGGCTGA ACTCGATCCC 
TTCCTTAATG TGACTCTTGA CGACGGGTCG GTGTCCCGCA GACACTTTAA AAACGAACTA 
ATCGTCCAGT GGGACCTACC GATGCCGTGT GACGGAATGT TCTACCTAGA CAACCGCGAA 
GAACAGGATA AGTACACGTT GTTCGAGAAC GGAACTTTCT TTCGCCACTT TGACCGTGTG 
ACTCTTCGCA AGCGGGAATA CTGCCTTCAG CATCTTACAT TCGCAGATGG TAATGCTACG 
TCTATTCGAA TTGCACCTCA CAACTGTTTG ATAGTGCCAT CAATTACCGG TCAGACGGTT 
GTGATGATCT CTTCGCTGAT ATGCATGGTT CTAACGATCG CCGTATACCT CTTCGTCAAG 
AAACTACAAA ACTTGCATGG AAAATGCTTC ATCTGCTACA TGGTGTGTCT CTTTATGGGA 
TATCTTTTCC TATTGCTCGA TTTGTGGCAG ATATCCATTA GCTTTTGCAA ACCAGCAGGT 
TTTCTGGGTT ACTTCTTTGT CATGGCCGCA TTTTTTTGGC TTTCCGTCAT CAGTCTGCAC 
CTTTGGAACA CGTTCAGAGG CTCCTCCCAC AAAGCGAATC GCTTCTTATT TGAGCATCGG 
TTTCTGGCCT ACAATACCTA TGCTTGGGGC ATGGCGGTGG TCCTGACAGG AATTACCGTT 
CTGGCCGATA ACATCGTGGA AAACCAGGAT TGGAATCCTC GTGTGGGCCA CGAGGGACAC 
TGTTGGATAT ATACTCAAGC CTGGTCAGCC ATGCTCTACT TTTACGGTCC AATGGTATTT 
CTTATTGCCT TTAACATAAC CATGTTCATC CTGACGGCTA AGCGTATATT AGGAGTGAAG 
AAGGACATTC AGAACTTTGC GCACAGGCAA GAGAGAAAGC AGAAGCTGAA CTCCGACAAA 
CAGACTTACA CCTTCTTCCT ACGGCTCTTC ATCATTATGG GATTGTCCTG GAGCTTGGAG 
ATAGGCTCCT ACTTTTCGCA ATCCAACCAA ACTTGGGCCA ACGTCTTTCT GGTGGCTGAC 
TATTTAAATT GGTCTCAAGG AATCATCATA TTTATACTGT TCGTTCTGAA GCGCAGCACG 
TGGAGACTCT TGCAGGAGAG GAACCATCCT AAATCCACGA GGAGTGTCAT TTCTAACAGA 
TCTATGGCAA GTCGTATTAC CGTGGGAACA ACTCCTAAAA ATAAAGCACG GAACTCGCCA 
TTGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728521.1
CDS	148..1716
Translation	MKTLLVLRISTVILVVLVIQKSYADILECDYFDTVDISAAQKLQNGSYLFEGLLVPAILTGEYDFRILPDDSKQKVARHIRGCVCKLKPCVRFCCPHDHIMDNGVCYDNMSDEELAELDPFLNVTLDDGSVSRRHFKNELIVQWDLPMPCDGMFYLDNREEQDKYTLFENGTFFRHFDRVTLRKREYCLQHLTFADGNATSIRIAPHNCLIVPSITGQTVVMISSLICMVLTIAVYLFVKKLQNLHGKCFICYMVCLFMGYLFLLLDLWQISISFCKPAGFLGYFFVMAAFFWLSVISLHLWNTFRGSSHKANRFLFEHRFLAYNTYAWGMAVVLTGITVLADNIVENQDWNPRVGHEGHCWIYTQAWSAMLYFYGPMVFLIAFNITMFILTAKRILGVKKDIQNFAHRQERKQKLNSDKQTYTFFLRLFIIMGLSWSLEIGSYFSQSNQTWANVFLVADYLNWSQGIIIFILFVLKRSTWRLLQERNHPKSTRSVISNRSMASRITVGTTPKNKARNSPLA

Target ORF information:

RefSeq Version	NM_167829.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster methuselah (mth), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167829.3

ORF Insert Sequence:

ATGAAAACTC TTTTGGTTCT TCGGATATCA ACTGTCATAC TTGTTGTGCT GGTTATTCAA 
AAATCATATG CAGACATTCT CGAATGCGAT TATTTCGACA CTGTCGATAT TTCGGCAGCT 
CAAAAACTAC AGAATGGATC GTACTTATTT GAGGGGTTGC TCGTTCCGGC CATTTTGACG 
GGAGAATATG ACTTTAGGAT TCTCCCGGAC GACTCGAAGC AGAAGGTGGC TCGTCACATA 
AGAGGATGTG TGTGCAAGCT GAAGCCCTGT GTCAGATTTT GCTGCCCTCA CGACCATATC 
ATGGATAATG GTGTCTGCTA CGACAACATG TCCGACGAGG AGCTGGCTGA ACTCGATCCC 
TTCCTTAATG TGACTCTTGA CGACGGGTCG GTGTCCCGCA GACACTTTAA AAACGAACTA 
ATCGTCCAGT GGGACCTACC GATGCCGTGT GACGGAATGT TCTACCTAGA CAACCGCGAA 
GAACAGGATA AGTACACGTT GTTCGAGAAC GGAACTTTCT TTCGCCACTT TGACCGTGTG 
ACTCTTCGCA AGCGGGAATA CTGCCTTCAG CATCTTACAT TCGCAGATGG TAATGCTACG 
TCTATTCGAA TTGCACCTCA CAACTGTTTG ATAGTGCCAT CAATTACCGG TCAGACGGTT 
GTGATGATCT CTTCGCTGAT ATGCATGGTT CTAACGATCG CCGTATACCT CTTCGTCAAG 
AAACTACAAA ACTTGCATGG AAAATGCTTC ATCTGCTACA TGGTGTGTCT CTTTATGGGA 
TATCTTTTCC TATTGCTCGA TTTGTGGCAG ATATCCATTA GCTTTTGCAA ACCAGCAGGT 
TTTCTGGGTT ACTTCTTTGT CATGGCCGCA TTTTTTTGGC TTTCCGTCAT CAGTCTGCAC 
CTTTGGAACA CGTTCAGAGG CTCCTCCCAC AAAGCGAATC GCTTCTTATT TGAGCATCGG 
TTTCTGGCCT ACAATACCTA TGCTTGGGGC ATGGCGGTGG TCCTGACAGG AATTACCGTT 
CTGGCCGATA ACATCGTGGA AAACCAGGAT TGGAATCCTC GTGTGGGCCA CGAGGGACAC 
TGTTGGATAT ATACTCAAGC CTGGTCAGCC ATGCTCTACT TTTACGGTCC AATGGTATTT 
CTTATTGCCT TTAACATAAC CATGTTCATC CTGACGGCTA AGCGTATATT AGGAGTGAAG 
AAGGACATTC AGAACTTTGC GCACAGGCAA GAGAGAAAGC AGAAGCTGAA CTCCGACAAA 
CAGACTTACA CCTTCTTCCT ACGGCTCTTC ATCATTATGG GATTGTCCTG GAGCTTGGAG 
ATAGGCTCCT ACTTTTCGCA ATCCAACCAA ACTTGGGCCA ACGTCTTTCT GGTGGCTGAC 
TATTTAAATT GGTCTCAAGG AATCATCATA TTTATACTGT TCGTTCTGAA GCGCAGCACG 
TGGAGACTCT TGCAGGAGAG GAACCATCCT AAATCCACGA GGAGTGTCAT TTCTAACAGA 
TCTATGGCAA GTCGTATTAC CGTGGGAACA ACTCCTAAAA ATAAAGCACG GAACTCGCCA 
TTGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mth ( NM_079147.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523871.1 Drosophila melanogaster methuselah (mth), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17066
Clone ID Related Accession (Same CDS sequence)	NM_079147.4 , NM_001259606.2
Accession Version	NM_079147.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	methuselah, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079147.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAACTC TTTTGGTTCT TCGGATATCA ACTGTCATAC TTGTTGTGCT GGTTATTCAA 
AAATCATATG CAGACATTCT CGAATGCGAT TATTTCGACA CTGTCGATAT TTCGGCAGCT 
CAAAAACTAC AGAATGGATC GTACTTATTT GAGGGGTTGC TCGTTCCGGC CATTTTGACG 
GGAGAATATG ACTTTAGGAT TCTCCCGGAC GACTCGAAGC AGAAGGTGGC TCGTCACATA 
AGAGGATGTG TGTGCAAGCT GAAGCCCTGT GTCAGATTTT GCTGCCCTCA CGACCATATC 
ATGGATAATG GTGTCTGCTA CGACAACATG TCCGACGAGG AGCTGGCTGA ACTCGATCCC 
TTCCTTAATG TGACTCTTGA CGACGGGTCG GTGTCCCGCA GACACTTTAA AAACGAACTA 
ATCGTCCAGT GGGACCTACC GATGCCGTGT GACGGAATGT TCTACCTAGA CAACCGCGAA 
GAACAGGATA AGTACACGTT GTTCGAGAAC GGAACTTTCT TTCGCCACTT TGACCGTGTG 
ACTCTTCGCA AGCGGGAATA CTGCCTTCAG CATCTTACAT TCGCAGATGG TAATGCTACG 
TCTATTCGAA TTGCACCTCA CAACTGTTTG ATAGTGCCAT CAATTACCGG TCAGACGGTT 
GTGATGATCT CTTCGCTGAT ATGCATGGTT CTAACGATCG CCGTATACCT CTTCGTCAAG 
AAACTACAAA ACTTGCATGG AAAATGCTTC ATCTGCTACA TGGTGTGTCT CTTTATGGGA 
TATCTTTTCC TATTGCTCGA TTTGTGGCAG ATATCCATTA GCTTTTGCAA ACCAGCAGGT 
TTTCTGGGTT ACTTCTTTGT CATGGCCGCA TTTTTTTGGC TTTCCGTCAT CAGTCTGCAC 
CTTTGGAACA CGTTCAGAGG CTCCTCCCAC AAAGCGAATC GCTTCTTATT TGAGCATCGG 
TTTCTGGCCT ACAATACCTA TGCTTGGGGC ATGGCGGTGG TCCTGACAGG AATTACCGTT 
CTGGCCGATA ACATCGTGGA AAACCAGGAT TGGAATCCTC GTGTGGGCCA CGAGGGACAC 
TGTTGGATAT ATACTCAAGC CTGGTCAGCC ATGCTCTACT TTTACGGTCC AATGGTATTT 
CTTATTGCCT TTAACATAAC CATGTTCATC CTGACGGCTA AGCGTATATT AGGAGTGAAG 
AAGGACATTC AGAACTTTGC GCACAGGCAA GAGAGAAAGC AGAAGCTGAA CTCCGACAAA 
CAGACTTACA CCTTCTTCCT ACGGCTCTTC ATCATTATGG GATTGTCCTG GAGCTTGGAG 
ATAGGCTCCT ACTTTTCGCA ATCCAACCAA ACTTGGGCCA ACGTCTTTCT GGTGGCTGAC 
TATTTAAATT GGTCTCAAGG AATCATCATA TTTATACTGT TCGTTCTGAA GCGCAGCACG 
TGGAGACTCT TGCAGGAGAG CATTAGGGGG GAGGGTGAGG AGGTAAACAA CAGTGAGGAA 
GAGATTTCGC TAGAAAACAC GACGACACGA AATGTCCTAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523871.1
CDS	148..1692
Translation	MKTLLVLRISTVILVVLVIQKSYADILECDYFDTVDISAAQKLQNGSYLFEGLLVPAILTGEYDFRILPDDSKQKVARHIRGCVCKLKPCVRFCCPHDHIMDNGVCYDNMSDEELAELDPFLNVTLDDGSVSRRHFKNELIVQWDLPMPCDGMFYLDNREEQDKYTLFENGTFFRHFDRVTLRKREYCLQHLTFADGNATSIRIAPHNCLIVPSITGQTVVMISSLICMVLTIAVYLFVKKLQNLHGKCFICYMVCLFMGYLFLLLDLWQISISFCKPAGFLGYFFVMAAFFWLSVISLHLWNTFRGSSHKANRFLFEHRFLAYNTYAWGMAVVLTGITVLADNIVENQDWNPRVGHEGHCWIYTQAWSAMLYFYGPMVFLIAFNITMFILTAKRILGVKKDIQNFAHRQERKQKLNSDKQTYTFFLRLFIIMGLSWSLEIGSYFSQSNQTWANVFLVADYLNWSQGIIIFILFVLKRSTWRLLQESIRGEGEEVNNSEEEISLENTTTRNVLL

Target ORF information:

RefSeq Version	NM_079147.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster methuselah (mth), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079147.4

ORF Insert Sequence:

ATGAAAACTC TTTTGGTTCT TCGGATATCA ACTGTCATAC TTGTTGTGCT GGTTATTCAA 
AAATCATATG CAGACATTCT CGAATGCGAT TATTTCGACA CTGTCGATAT TTCGGCAGCT 
CAAAAACTAC AGAATGGATC GTACTTATTT GAGGGGTTGC TCGTTCCGGC CATTTTGACG 
GGAGAATATG ACTTTAGGAT TCTCCCGGAC GACTCGAAGC AGAAGGTGGC TCGTCACATA 
AGAGGATGTG TGTGCAAGCT GAAGCCCTGT GTCAGATTTT GCTGCCCTCA CGACCATATC 
ATGGATAATG GTGTCTGCTA CGACAACATG TCCGACGAGG AGCTGGCTGA ACTCGATCCC 
TTCCTTAATG TGACTCTTGA CGACGGGTCG GTGTCCCGCA GACACTTTAA AAACGAACTA 
ATCGTCCAGT GGGACCTACC GATGCCGTGT GACGGAATGT TCTACCTAGA CAACCGCGAA 
GAACAGGATA AGTACACGTT GTTCGAGAAC GGAACTTTCT TTCGCCACTT TGACCGTGTG 
ACTCTTCGCA AGCGGGAATA CTGCCTTCAG CATCTTACAT TCGCAGATGG TAATGCTACG 
TCTATTCGAA TTGCACCTCA CAACTGTTTG ATAGTGCCAT CAATTACCGG TCAGACGGTT 
GTGATGATCT CTTCGCTGAT ATGCATGGTT CTAACGATCG CCGTATACCT CTTCGTCAAG 
AAACTACAAA ACTTGCATGG AAAATGCTTC ATCTGCTACA TGGTGTGTCT CTTTATGGGA 
TATCTTTTCC TATTGCTCGA TTTGTGGCAG ATATCCATTA GCTTTTGCAA ACCAGCAGGT 
TTTCTGGGTT ACTTCTTTGT CATGGCCGCA TTTTTTTGGC TTTCCGTCAT CAGTCTGCAC 
CTTTGGAACA CGTTCAGAGG CTCCTCCCAC AAAGCGAATC GCTTCTTATT TGAGCATCGG 
TTTCTGGCCT ACAATACCTA TGCTTGGGGC ATGGCGGTGG TCCTGACAGG AATTACCGTT 
CTGGCCGATA ACATCGTGGA AAACCAGGAT TGGAATCCTC GTGTGGGCCA CGAGGGACAC 
TGTTGGATAT ATACTCAAGC CTGGTCAGCC ATGCTCTACT TTTACGGTCC AATGGTATTT 
CTTATTGCCT TTAACATAAC CATGTTCATC CTGACGGCTA AGCGTATATT AGGAGTGAAG 
AAGGACATTC AGAACTTTGC GCACAGGCAA GAGAGAAAGC AGAAGCTGAA CTCCGACAAA 
CAGACTTACA CCTTCTTCCT ACGGCTCTTC ATCATTATGG GATTGTCCTG GAGCTTGGAG 
ATAGGCTCCT ACTTTTCGCA ATCCAACCAA ACTTGGGCCA ACGTCTTTCT GGTGGCTGAC 
TATTTAAATT GGTCTCAAGG AATCATCATA TTTATACTGT TCGTTCTGAA GCGCAGCACG 
TGGAGACTCT TGCAGGAGAG CATTAGGGGG GAGGGTGAGG AGGTAAACAA CAGTGAGGAA 
GAGATTTCGC TAGAAAACAC GACGACACGA AATGTCCTAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mth ( NM_001259606.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246535.1 Drosophila melanogaster methuselah (mth), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa17066
Clone ID Related Accession (Same CDS sequence)	NM_079147.4 , NM_001259606.2
Accession Version	NM_001259606.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	methuselah, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259606.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAACTC TTTTGGTTCT TCGGATATCA ACTGTCATAC TTGTTGTGCT GGTTATTCAA 
AAATCATATG CAGACATTCT CGAATGCGAT TATTTCGACA CTGTCGATAT TTCGGCAGCT 
CAAAAACTAC AGAATGGATC GTACTTATTT GAGGGGTTGC TCGTTCCGGC CATTTTGACG 
GGAGAATATG ACTTTAGGAT TCTCCCGGAC GACTCGAAGC AGAAGGTGGC TCGTCACATA 
AGAGGATGTG TGTGCAAGCT GAAGCCCTGT GTCAGATTTT GCTGCCCTCA CGACCATATC 
ATGGATAATG GTGTCTGCTA CGACAACATG TCCGACGAGG AGCTGGCTGA ACTCGATCCC 
TTCCTTAATG TGACTCTTGA CGACGGGTCG GTGTCCCGCA GACACTTTAA AAACGAACTA 
ATCGTCCAGT GGGACCTACC GATGCCGTGT GACGGAATGT TCTACCTAGA CAACCGCGAA 
GAACAGGATA AGTACACGTT GTTCGAGAAC GGAACTTTCT TTCGCCACTT TGACCGTGTG 
ACTCTTCGCA AGCGGGAATA CTGCCTTCAG CATCTTACAT TCGCAGATGG TAATGCTACG 
TCTATTCGAA TTGCACCTCA CAACTGTTTG ATAGTGCCAT CAATTACCGG TCAGACGGTT 
GTGATGATCT CTTCGCTGAT ATGCATGGTT CTAACGATCG CCGTATACCT CTTCGTCAAG 
AAACTACAAA ACTTGCATGG AAAATGCTTC ATCTGCTACA TGGTGTGTCT CTTTATGGGA 
TATCTTTTCC TATTGCTCGA TTTGTGGCAG ATATCCATTA GCTTTTGCAA ACCAGCAGGT 
TTTCTGGGTT ACTTCTTTGT CATGGCCGCA TTTTTTTGGC TTTCCGTCAT CAGTCTGCAC 
CTTTGGAACA CGTTCAGAGG CTCCTCCCAC AAAGCGAATC GCTTCTTATT TGAGCATCGG 
TTTCTGGCCT ACAATACCTA TGCTTGGGGC ATGGCGGTGG TCCTGACAGG AATTACCGTT 
CTGGCCGATA ACATCGTGGA AAACCAGGAT TGGAATCCTC GTGTGGGCCA CGAGGGACAC 
TGTTGGATAT ATACTCAAGC CTGGTCAGCC ATGCTCTACT TTTACGGTCC AATGGTATTT 
CTTATTGCCT TTAACATAAC CATGTTCATC CTGACGGCTA AGCGTATATT AGGAGTGAAG 
AAGGACATTC AGAACTTTGC GCACAGGCAA GAGAGAAAGC AGAAGCTGAA CTCCGACAAA 
CAGACTTACA CCTTCTTCCT ACGGCTCTTC ATCATTATGG GATTGTCCTG GAGCTTGGAG 
ATAGGCTCCT ACTTTTCGCA ATCCAACCAA ACTTGGGCCA ACGTCTTTCT GGTGGCTGAC 
TATTTAAATT GGTCTCAAGG AATCATCATA TTTATACTGT TCGTTCTGAA GCGCAGCACG 
TGGAGACTCT TGCAGGAGAG CATTAGGGGG GAGGGTGAGG AGGTAAACAA CAGTGAGGAA 
GAGATTTCGC TAGAAAACAC GACGACACGA AATGTCCTAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246535.1
CDS	601..2145
Translation	MKTLLVLRISTVILVVLVIQKSYADILECDYFDTVDISAAQKLQNGSYLFEGLLVPAILTGEYDFRILPDDSKQKVARHIRGCVCKLKPCVRFCCPHDHIMDNGVCYDNMSDEELAELDPFLNVTLDDGSVSRRHFKNELIVQWDLPMPCDGMFYLDNREEQDKYTLFENGTFFRHFDRVTLRKREYCLQHLTFADGNATSIRIAPHNCLIVPSITGQTVVMISSLICMVLTIAVYLFVKKLQNLHGKCFICYMVCLFMGYLFLLLDLWQISISFCKPAGFLGYFFVMAAFFWLSVISLHLWNTFRGSSHKANRFLFEHRFLAYNTYAWGMAVVLTGITVLADNIVENQDWNPRVGHEGHCWIYTQAWSAMLYFYGPMVFLIAFNITMFILTAKRILGVKKDIQNFAHRQERKQKLNSDKQTYTFFLRLFIIMGLSWSLEIGSYFSQSNQTWANVFLVADYLNWSQGIIIFILFVLKRSTWRLLQESIRGEGEEVNNSEEEISLENTTTRNVLL

Target ORF information:

RefSeq Version	NM_001259606.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster methuselah (mth), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259606.2

ORF Insert Sequence:

ATGAAAACTC TTTTGGTTCT TCGGATATCA ACTGTCATAC TTGTTGTGCT GGTTATTCAA 
AAATCATATG CAGACATTCT CGAATGCGAT TATTTCGACA CTGTCGATAT TTCGGCAGCT 
CAAAAACTAC AGAATGGATC GTACTTATTT GAGGGGTTGC TCGTTCCGGC CATTTTGACG 
GGAGAATATG ACTTTAGGAT TCTCCCGGAC GACTCGAAGC AGAAGGTGGC TCGTCACATA 
AGAGGATGTG TGTGCAAGCT GAAGCCCTGT GTCAGATTTT GCTGCCCTCA CGACCATATC 
ATGGATAATG GTGTCTGCTA CGACAACATG TCCGACGAGG AGCTGGCTGA ACTCGATCCC 
TTCCTTAATG TGACTCTTGA CGACGGGTCG GTGTCCCGCA GACACTTTAA AAACGAACTA 
ATCGTCCAGT GGGACCTACC GATGCCGTGT GACGGAATGT TCTACCTAGA CAACCGCGAA 
GAACAGGATA AGTACACGTT GTTCGAGAAC GGAACTTTCT TTCGCCACTT TGACCGTGTG 
ACTCTTCGCA AGCGGGAATA CTGCCTTCAG CATCTTACAT TCGCAGATGG TAATGCTACG 
TCTATTCGAA TTGCACCTCA CAACTGTTTG ATAGTGCCAT CAATTACCGG TCAGACGGTT 
GTGATGATCT CTTCGCTGAT ATGCATGGTT CTAACGATCG CCGTATACCT CTTCGTCAAG 
AAACTACAAA ACTTGCATGG AAAATGCTTC ATCTGCTACA TGGTGTGTCT CTTTATGGGA 
TATCTTTTCC TATTGCTCGA TTTGTGGCAG ATATCCATTA GCTTTTGCAA ACCAGCAGGT 
TTTCTGGGTT ACTTCTTTGT CATGGCCGCA TTTTTTTGGC TTTCCGTCAT CAGTCTGCAC 
CTTTGGAACA CGTTCAGAGG CTCCTCCCAC AAAGCGAATC GCTTCTTATT TGAGCATCGG 
TTTCTGGCCT ACAATACCTA TGCTTGGGGC ATGGCGGTGG TCCTGACAGG AATTACCGTT 
CTGGCCGATA ACATCGTGGA AAACCAGGAT TGGAATCCTC GTGTGGGCCA CGAGGGACAC 
TGTTGGATAT ATACTCAAGC CTGGTCAGCC ATGCTCTACT TTTACGGTCC AATGGTATTT 
CTTATTGCCT TTAACATAAC CATGTTCATC CTGACGGCTA AGCGTATATT AGGAGTGAAG 
AAGGACATTC AGAACTTTGC GCACAGGCAA GAGAGAAAGC AGAAGCTGAA CTCCGACAAA 
CAGACTTACA CCTTCTTCCT ACGGCTCTTC ATCATTATGG GATTGTCCTG GAGCTTGGAG 
ATAGGCTCCT ACTTTTCGCA ATCCAACCAA ACTTGGGCCA ACGTCTTTCT GGTGGCTGAC 
TATTTAAATT GGTCTCAAGG AATCATCATA TTTATACTGT TCGTTCTGAA GCGCAGCACG 
TGGAGACTCT TGCAGGAGAG CATTAGGGGG GAGGGTGAGG AGGTAAACAA CAGTGAGGAA 
GAGATTTCGC TAGAAAACAC GACGACACGA AATGTCCTAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mth cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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ORF Insert Sequence:

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