PheRS-m cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PheRS-m
Entrez Gene ID	36547
Full Name	Phenylalanyl-tRNA synthetase, mitochondrial
Synonyms	Aats-phe,Aats-phe-m,CG13348,Dmel\CG13348,FRS,PheRS,Pts,mtPheRS
General protein information	Preferred Names Phenylalanyl-tRNA synthetase, mitochondrial Names CG13348 gene product from transcript CG13348-RA CG13348-PA PheRS-m-PA Phenylalanyl-tRNA synthetase mitochondrial phenylalanyl-tRNA synthetase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 50C20-50C20

mRNA and Protein(s)

mRNA	Protein	Name
NM_057935.5	NP_477283.1	Phenylalanyl-tRNA synthetase, mitochondrial

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0B12177g	XP_452072.1
Gallus gallus (chicken)	FARS2	XP_426039.2
Caenorhabditis elegans (roundworm)	fars-2	NP_507852.2
Xenopus tropicalis (tropical clawed frog)	fars2	XP_002936470.2
Pan troglodytes (chimpanzee)	FARS2	NP_001233392.1
Canis lupus familiaris (dog)	FARS2	XP_545326.3
Schizosaccharomyces pombe (fission yeast)	SPCC736.03c	NP_587774.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012096	XP_320432.3
Mus musculus (house mouse)	Fars2	NP_077236.1
Eremothecium gossypii	AGOS_ADL305C	NP_983791.2
Homo sapiens (human)	FARS2	NP_006558.1
Drosophila melanogaster (fruit fly)	Aats-phe	NP_477283.1
Arabidopsis thaliana (thale cress)	AT3G58140	NP_567061.1
Neurospora crassa	NCU01512	XP_956961.1
Magnaporthe oryzae (rice blast fungus)	MGG_03217	XP_003716762.1
Oryza sativa (rice)	Os12g0533700	NP_001066926.1
Bos taurus (cattle)	FARS2	NP_001070273.1
Rattus norvegicus (Norway rat)	Fars2	NP_001013157.1
Danio rerio (zebrafish)	fars2	NP_001099064.1
Saccharomyces cerevisiae (baker's yeast)	MSF1	NP_015372.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PheRS-m gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PheRS-m cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12524	NM_057935.5 Latest version!	Drosophila melanogaster Phenylalanyl-tRNA synthetase, mitochondrial (PheRS-m), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PheRS-m ( NM_057935.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477283.1 Drosophila melanogaster Phenylalanyl-tRNA synthetase, mitochondrial (PheRS-m), mRNA.

Price & Availability↑

CloneID	OFa12524
Clone ID Related Accession (Same CDS sequence)	NM_057935.5
Accession Version	NM_057935.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Phenylalanyl-tRNA synthetase, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057935.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTCTGA CGCTTCGAGT GCAGGGAGCA CGGCACTGGT TGAAGTCCAC CCGCTGCCTG 
GCCAGCAGTG CGGCGCCCGC GAAGTCGCCG TCATCGCCAC CGCAGCTGGA GGTCAGTGGA 
AGCACGTACG CCACCGACGG ATGGACGAAC GTAACGCCCA AGATACTCTC CTACGTGGGG 
GCCAACAAGC ACCTGCAGAC GGACCACCCG CTTTCCATCA TCCGCCAGAG GATCGTAAAC 
TACTTTTATG GAGCGTATCG CAACCAAAGG GGCAATCCAC TATTCTCCGT CTATGATCAA 
ATGAATCCGG TGGTTACTGT GCAGCAGAAC TTTGACAATC TGCTAATTCC CGCCGATCAT 
GTGAGTCGAC AGAAATCCGA TTGCTATTAC ATCAATCAGC AGCATCTGCT GCGCGCCCAC 
ACCACCGCCC ATCAGGTGGA ATTGATCTCG GGCGGGCTGG ACAACTTTCT GGTCGTGGGC 
GAGGTGTATC GACGGGACGA AATCGATAGC ACCCACTATC CGGTATTCCA CCAGGCGGAC 
GCTGTGCGGC TGGTCACCAA AGACAAGCTT TTCGAGCGCA ATCCTGGCCT GGAACTCTTT 
GAAGAGACGT GGTCGGGTAC GCTGGCCGAC CCCAAGCTGA TTCTGCCCTC ATCCAAGTTC 
ATGGACCAAA CCAAACAGCC CTGCCACACG CTCGAGGCCG TCAAGTTGAT GGAGCACGAG 
ATGAAGCACG TGCTGGTTGG CCTGACCAAG GATCTGTTTG GGCCGCGCAT CAAGTACAGA 
TGGGTGGACA CCTATTTTCC CTTCACCCAG CCCTCCTGGG AGCTGGAGAT CTATTTCAAG 
GACAACTGGC TGGAGGTCTT AGGCTGCGGC ATCATGCGCC ACGAAATCCT GCAGAGATCG 
GGCGTTCATC AATCGATTGG CTACGCCTTT GGCGTCGGCC TGGAGCGACT GGCCATGGTG 
CTGTTCGATA TACCTGACAT CCGGCTCTTC TGGTCGAACG ACAGTGGCTT TCTGTCGCAG 
TTTAGCGAAA AGGATCTGCA CAATCTGCCC AAGTACAAGC CCATCTCCCA CTATCCGCAG 
TGCACGAATG ACTTGTCGTT CTGGCTGCCC CAGGACATTG AGGTGGATGC CGGCTTCTCG 
CCGAACGACT TTTACGATCT AGTCCGCTCT GTAGCTGGTG ATATGGTGGA GCAGATCAGT 
CTGGTGGACA AGTTTAAGCA CCCGAAAACG GGGAAATCGA GCGTGTGCTT CCGCATTGTT 
TATCGCCATA TGGAGCGCAC CTTAACCCAG GCGGAGGTCA ACGAAATCCA CAAGCAGATC 
GCTAGTGCGA GCGTGGATAG TTTCAATGTG CAAATACGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477283.1
CDS	102..1463
Translation	MLLTLRVQGARHWLKSTRCLASSAAPAKSPSSPPQLEVSGSTYATDGWTNVTPKILSYVGANKHLQTDHPLSIIRQRIVNYFYGAYRNQRGNPLFSVYDQMNPVVTVQQNFDNLLIPADHVSRQKSDCYYINQQHLLRAHTTAHQVELISGGLDNFLVVGEVYRRDEIDSTHYPVFHQADAVRLVTKDKLFERNPGLELFEETWSGTLADPKLILPSSKFMDQTKQPCHTLEAVKLMEHEMKHVLVGLTKDLFGPRIKYRWVDTYFPFTQPSWELEIYFKDNWLEVLGCGIMRHEILQRSGVHQSIGYAFGVGLERLAMVLFDIPDIRLFWSNDSGFLSQFSEKDLHNLPKYKPISHYPQCTNDLSFWLPQDIEVDAGFSPNDFYDLVRSVAGDMVEQISLVDKFKHPKTGKSSVCFRIVYRHMERTLTQAEVNEIHKQIASASVDSFNVQIR

Target ORF information:

RefSeq Version	NM_057935.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Phenylalanyl-tRNA synthetase, mitochondrial (PheRS-m), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057935.5

ORF Insert Sequence:

ATGCTTCTGA CGCTTCGAGT GCAGGGAGCA CGGCACTGGT TGAAGTCCAC CCGCTGCCTG 
GCCAGCAGTG CGGCGCCCGC GAAGTCGCCG TCATCGCCAC CGCAGCTGGA GGTCAGTGGA 
AGCACGTACG CCACCGACGG ATGGACGAAC GTAACGCCCA AGATACTCTC CTACGTGGGG 
GCCAACAAGC ACCTGCAGAC GGACCACCCG CTTTCCATCA TCCGCCAGAG GATCGTAAAC 
TACTTTTATG GAGCGTATCG CAACCAAAGG GGCAATCCAC TATTCTCCGT CTATGATCAA 
ATGAATCCGG TGGTTACTGT GCAGCAGAAC TTTGACAATC TGCTAATTCC CGCCGATCAT 
GTGAGTCGAC AGAAATCCGA TTGCTATTAC ATCAATCAGC AGCATCTGCT GCGCGCCCAC 
ACCACCGCCC ATCAGGTGGA ATTGATCTCG GGCGGGCTGG ACAACTTTCT GGTCGTGGGC 
GAGGTGTATC GACGGGACGA AATCGATAGC ACCCACTATC CGGTATTCCA CCAGGCGGAC 
GCTGTGCGGC TGGTCACCAA AGACAAGCTT TTCGAGCGCA ATCCTGGCCT GGAACTCTTT 
GAAGAGACGT GGTCGGGTAC GCTGGCCGAC CCCAAGCTGA TTCTGCCCTC ATCCAAGTTC 
ATGGACCAAA CCAAACAGCC CTGCCACACG CTCGAGGCCG TCAAGTTGAT GGAGCACGAG 
ATGAAGCACG TGCTGGTTGG CCTGACCAAG GATCTGTTTG GGCCGCGCAT CAAGTACAGA 
TGGGTGGACA CCTATTTTCC CTTCACCCAG CCCTCCTGGG AGCTGGAGAT CTATTTCAAG 
GACAACTGGC TGGAGGTCTT AGGCTGCGGC ATCATGCGCC ACGAAATCCT GCAGAGATCG 
GGCGTTCATC AATCGATTGG CTACGCCTTT GGCGTCGGCC TGGAGCGACT GGCCATGGTG 
CTGTTCGATA TACCTGACAT CCGGCTCTTC TGGTCGAACG ACAGTGGCTT TCTGTCGCAG 
TTTAGCGAAA AGGATCTGCA CAATCTGCCC AAGTACAAGC CCATCTCCCA CTATCCGCAG 
TGCACGAATG ACTTGTCGTT CTGGCTGCCC CAGGACATTG AGGTGGATGC CGGCTTCTCG 
CCGAACGACT TTTACGATCT AGTCCGCTCT GTAGCTGGTG ATATGGTGGA GCAGATCAGT 
CTGGTGGACA AGTTTAAGCA CCCGAAAACG GGGAAATCGA GCGTGTGCTT CCGCATTGTT 
TATCGCCATA TGGAGCGCAC CTTAACCCAG GCGGAGGTCA ACGAAATCCA CAAGCAGATC 
GCTAGTGCGA GCGTGGATAG TTTCAATGTG CAAATACGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PheRS-m cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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