Gapdh1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gapdh1
Entrez Gene ID	35728
Full Name	Glyceraldehyde 3 phosphate dehydrogenase 1
Synonyms	BEST:GH12586,CG12055,Dmel\CG12055,GA3PDH,GADPH,GAP,GAPDH,GAPDH I,GAPDH-1,GAPDH1,GAPDHI,Gapd,Gapdh,Gapdh-1,Gapdh43E,gapdh,gapdh-1,gapdh1,gh12586
General protein information	Preferred Names Glyceraldehyde 3 phosphate dehydrogenase 1 Names CG12055 gene product from transcript CG12055-RA CG12055-PA CG12055-PB G3-P dehydrogenase GAPDH1 Gapdh1-PA Gapdh1-PB Glyceraldehyde-3-phosphate dehydrogenase1 glyceraldehyde 3 phosphate dehydrogenase glyceraldehyde 3 phosphate dehydrogenase 1 glyceraldehyde 3 phosphate dehydrogenase1 glyceraldehyde-3-phosphate dehydrogenase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 43E17-43E17

mRNA and Protein(s)

mRNA	Protein	Name
NM_080369.3	NP_525108.2	glyceraldehyde 3 phosphate dehydrogenase 1, isoform A
NM_001038847.2	NP_001033936.1	glyceraldehyde 3 phosphate dehydrogenase 1, isoform B

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GAPDH	NP_001029206.1
Mus musculus (house mouse)	Gapdh	NP_032110.1
Gallus gallus (chicken)	GAPDH	NP_989636.1
Caenorhabditis elegans (roundworm)	gpd-1	NP_496237.1
Caenorhabditis elegans (roundworm)	gpd-3	NP_508534.3
Saccharomyces cerevisiae (baker's yeast)	TDH2	NP_012542.1
Canis lupus familiaris (dog)	LOC100688969	XP_003435697.1
Mus musculus (house mouse)	Gm20899	XP_001476757.1
Kluyveromyces lactis	KLLA0F20988g	XP_456022.1
Oryza sativa (rice)	Os08g0126300	NP_001060897.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009623	XP_318655.2
Schizosaccharomyces pombe (fission yeast)	gpd3	NP_595236.1
Magnaporthe oryzae (rice blast fungus)	MGG_01084	XP_003717853.1
Caenorhabditis elegans (roundworm)	gpd-2	NP_508535.1
Drosophila melanogaster (fruit fly)	Gapdh1	NP_525108.2
Caenorhabditis elegans (roundworm)	gpd-4	NP_496192.1
Saccharomyces cerevisiae (baker's yeast)	TDH1	NP_012483.3
Schizosaccharomyces pombe (fission yeast)	tdh1	NP_596154.1
Macaca mulatta (Rhesus monkey)	GAPDH	XP_001105471.1
Rattus norvegicus (Norway rat)	Gapdh	NP_058704.1
Drosophila melanogaster (fruit fly)	Gapdh2	NP_001259584.1
Saccharomyces cerevisiae (baker's yeast)	TDH3	NP_011708.3
Canis lupus familiaris (dog)	LOC100683724	XP_003434435.1
Pan troglodytes (chimpanzee)	GAPDH	XP_508955.1
Canis lupus familiaris (dog)	GAPDH	NP_001003142.1
Neurospora crassa	NCU01528	XP_956977.1
Homo sapiens (human)	GAPDH	NP_002037.2
Danio rerio (zebrafish)	gapdh	NP_001108586.1
Xenopus tropicalis (tropical clawed frog)	gapdh	NP_001004949.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gapdh1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gapdh1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09195	NM_080369.3 Latest version!	Drosophila melanogaster glyceraldehyde 3 phosphate dehydrogenase 1, transcript variant A (Gapdh1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OFa09195	NM_001038847.2 Latest version!	Drosophila melanogaster glyceraldehyde 3 phosphate dehydrogenase 1, transcript variant B (Gapdh1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gapdh1 ( NM_080369.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525108.2 Drosophila melanogaster glyceraldehyde 3 phosphate dehydrogenase 1, transcript variant A (Gapdh1), mRNA.

Price & Availability↑

CloneID	OFa09195
Clone ID Related Accession (Same CDS sequence)	NM_080369.3 , NM_001038847.2
Accession Version	NM_080369.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	glyceraldehyde 3 phosphate dehydrogenase 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_080369.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAAGA TCGGAATTAA CGGATTTGGC CGCATCGGCC GCTTGGTGCT CCGCGCCGCC 
ATCGATAAGG GCGCCTCCGT GGTGGCCGTC AACGATCCCT TCATCGATGT CAACTACATG 
GTTTACCTGT TTAAATTCGA CTCGACTCAC GGTCGTTTCA AGGGCACCGT TGCGGCTGAG 
GGCGGATTCC TGGTGGTGAA CGGCCAGAAG ATCACCGTGT TCAGCGAGCG CGACCCGGCC 
AACATCAACT GGGCCAGTGC TGGAGCCGAG TATGTGGTGG AGTCCACCGG AGTGTTCACC 
ACCATTGACA AGGCGTCCAC CCACTTGAAG GGCGGCGCCA AGAAGGTCAT CATCTCGGCC 
CCATCCGCCG ATGCGCCCAT GTTCGTGTGC GGCGTTAACC TGGACGCCTA CAGCCCCGAC 
ATGAAGGTGG TCTCCAACGC CTCGTGCACC ACCAACTGCC TGGCTCCCCT GGCCAAGGTC 
ATCAATGACA ACTTCGAGAT CGTCGAGGGT CTGATGACCA CCGTGCACGC CACCACTGCC 
ACCCAGAAGA CCGTCGACGG TCCCTCTGGC AAACTGTGGC GCGATGGACG TGGCGCCGCC 
CAGAACATCA TCCCGGCCGC CACCGGAGCC GCCAAGGCTG TGGGCAAGGT CATCCCCGCC 
CTGAACGGCA AGCTGACCGG CATGGCTTTC CGCGTGCCCA CGCCCAATGT CTCCGTTGTG 
GATCTTACCG TCCGCTTGGG CAAGGGAGCC ACCTATGACG AAATCAAGGC TAAGGTCGAG 
GAGGCCTCCA AGGGACCCCT GAAGGGAATC CTGGGCTACA CCGATGAGGA GGTGGTCTCC 
ACCGACTTCT TCAGCGACAC CCATTCGTCT GTGTTCGACG CCAAGGCTGG CATTTCGCTG 
AACGATAAGT TCGTCAAGCT AATCTCGTGG TACGACAACG AGTTCGGTTA CTCCAACCGC 
GTCATCGACC TGATCAAGTA TATGCAGAGC AAGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525108.2
CDS	261..1259
Translation	MSKIGINGFGRIGRLVLRAAIDKGASVVAVNDPFIDVNYMVYLFKFDSTHGRFKGTVAAEGGFLVVNGQKITVFSERDPANINWASAGAEYVVESTGVFTTIDKASTHLKGGAKKVIISAPSADAPMFVCGVNLDAYSPDMKVVSNASCTTNCLAPLAKVINDNFEIVEGLMTTVHATTATQKTVDGPSGKLWRDGRGAAQNIIPAATGAAKAVGKVIPALNGKLTGMAFRVPTPNVSVVDLTVRLGKGATYDEIKAKVEEASKGPLKGILGYTDEEVVSTDFFSDTHSSVFDAKAGISLNDKFVKLISWYDNEFGYSNRVIDLIKYMQSKD

Target ORF information:

RefSeq Version	NM_080369.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glyceraldehyde 3 phosphate dehydrogenase 1, transcript variant A (Gapdh1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080369.3

ORF Insert Sequence:

ATGTCGAAGA TCGGAATTAA CGGATTTGGC CGCATCGGCC GCTTGGTGCT CCGCGCCGCC 
ATCGATAAGG GCGCCTCCGT GGTGGCCGTC AACGATCCCT TCATCGATGT CAACTACATG 
GTTTACCTGT TTAAATTCGA CTCGACTCAC GGTCGTTTCA AGGGCACCGT TGCGGCTGAG 
GGCGGATTCC TGGTGGTGAA CGGCCAGAAG ATCACCGTGT TCAGCGAGCG CGACCCGGCC 
AACATCAACT GGGCCAGTGC TGGAGCCGAG TATGTGGTGG AGTCCACCGG AGTGTTCACC 
ACCATTGACA AGGCGTCCAC CCACTTGAAG GGCGGCGCCA AGAAGGTCAT CATCTCGGCC 
CCATCCGCCG ATGCGCCCAT GTTCGTGTGC GGCGTTAACC TGGACGCCTA CAGCCCCGAC 
ATGAAGGTGG TCTCCAACGC CTCGTGCACC ACCAACTGCC TGGCTCCCCT GGCCAAGGTC 
ATCAATGACA ACTTCGAGAT CGTCGAGGGT CTGATGACCA CCGTGCACGC CACCACTGCC 
ACCCAGAAGA CCGTCGACGG TCCCTCTGGC AAACTGTGGC GCGATGGACG TGGCGCCGCC 
CAGAACATCA TCCCGGCCGC CACCGGAGCC GCCAAGGCTG TGGGCAAGGT CATCCCCGCC 
CTGAACGGCA AGCTGACCGG CATGGCTTTC CGCGTGCCCA CGCCCAATGT CTCCGTTGTG 
GATCTTACCG TCCGCTTGGG CAAGGGAGCC ACCTATGACG AAATCAAGGC TAAGGTCGAG 
GAGGCCTCCA AGGGACCCCT GAAGGGAATC CTGGGCTACA CCGATGAGGA GGTGGTCTCC 
ACCGACTTCT TCAGCGACAC CCATTCGTCT GTGTTCGACG CCAAGGCTGG CATTTCGCTG 
AACGATAAGT TCGTCAAGCT AATCTCGTGG TACGACAACG AGTTCGGTTA CTCCAACCGC 
GTCATCGACC TGATCAAGTA TATGCAGAGC AAGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gapdh1 ( NM_001038847.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001033936.1 Drosophila melanogaster glyceraldehyde 3 phosphate dehydrogenase 1, transcript variant B (Gapdh1), mRNA.

Price & Availability↑

CloneID	OFa09195
Clone ID Related Accession (Same CDS sequence)	NM_080369.3 , NM_001038847.2
Accession Version	NM_001038847.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	glyceraldehyde 3 phosphate dehydrogenase 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001038847.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAAGA TCGGAATTAA CGGATTTGGC CGCATCGGCC GCTTGGTGCT CCGCGCCGCC 
ATCGATAAGG GCGCCTCCGT GGTGGCCGTC AACGATCCCT TCATCGATGT CAACTACATG 
GTTTACCTGT TTAAATTCGA CTCGACTCAC GGTCGTTTCA AGGGCACCGT TGCGGCTGAG 
GGCGGATTCC TGGTGGTGAA CGGCCAGAAG ATCACCGTGT TCAGCGAGCG CGACCCGGCC 
AACATCAACT GGGCCAGTGC TGGAGCCGAG TATGTGGTGG AGTCCACCGG AGTGTTCACC 
ACCATTGACA AGGCGTCCAC CCACTTGAAG GGCGGCGCCA AGAAGGTCAT CATCTCGGCC 
CCATCCGCCG ATGCGCCCAT GTTCGTGTGC GGCGTTAACC TGGACGCCTA CAGCCCCGAC 
ATGAAGGTGG TCTCCAACGC CTCGTGCACC ACCAACTGCC TGGCTCCCCT GGCCAAGGTC 
ATCAATGACA ACTTCGAGAT CGTCGAGGGT CTGATGACCA CCGTGCACGC CACCACTGCC 
ACCCAGAAGA CCGTCGACGG TCCCTCTGGC AAACTGTGGC GCGATGGACG TGGCGCCGCC 
CAGAACATCA TCCCGGCCGC CACCGGAGCC GCCAAGGCTG TGGGCAAGGT CATCCCCGCC 
CTGAACGGCA AGCTGACCGG CATGGCTTTC CGCGTGCCCA CGCCCAATGT CTCCGTTGTG 
GATCTTACCG TCCGCTTGGG CAAGGGAGCC ACCTATGACG AAATCAAGGC TAAGGTCGAG 
GAGGCCTCCA AGGGACCCCT GAAGGGAATC CTGGGCTACA CCGATGAGGA GGTGGTCTCC 
ACCGACTTCT TCAGCGACAC CCATTCGTCT GTGTTCGACG CCAAGGCTGG CATTTCGCTG 
AACGATAAGT TCGTCAAGCT AATCTCGTGG TACGACAACG AGTTCGGTTA CTCCAACCGC 
GTCATCGACC TGATCAAGTA TATGCAGAGC AAGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033936.1
CDS	111..1109
Translation	MSKIGINGFGRIGRLVLRAAIDKGASVVAVNDPFIDVNYMVYLFKFDSTHGRFKGTVAAEGGFLVVNGQKITVFSERDPANINWASAGAEYVVESTGVFTTIDKASTHLKGGAKKVIISAPSADAPMFVCGVNLDAYSPDMKVVSNASCTTNCLAPLAKVINDNFEIVEGLMTTVHATTATQKTVDGPSGKLWRDGRGAAQNIIPAATGAAKAVGKVIPALNGKLTGMAFRVPTPNVSVVDLTVRLGKGATYDEIKAKVEEASKGPLKGILGYTDEEVVSTDFFSDTHSSVFDAKAGISLNDKFVKLISWYDNEFGYSNRVIDLIKYMQSKD

Target ORF information:

RefSeq Version	NM_001038847.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glyceraldehyde 3 phosphate dehydrogenase 1, transcript variant B (Gapdh1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038847.2

ORF Insert Sequence:

ATGTCGAAGA TCGGAATTAA CGGATTTGGC CGCATCGGCC GCTTGGTGCT CCGCGCCGCC 
ATCGATAAGG GCGCCTCCGT GGTGGCCGTC AACGATCCCT TCATCGATGT CAACTACATG 
GTTTACCTGT TTAAATTCGA CTCGACTCAC GGTCGTTTCA AGGGCACCGT TGCGGCTGAG 
GGCGGATTCC TGGTGGTGAA CGGCCAGAAG ATCACCGTGT TCAGCGAGCG CGACCCGGCC 
AACATCAACT GGGCCAGTGC TGGAGCCGAG TATGTGGTGG AGTCCACCGG AGTGTTCACC 
ACCATTGACA AGGCGTCCAC CCACTTGAAG GGCGGCGCCA AGAAGGTCAT CATCTCGGCC 
CCATCCGCCG ATGCGCCCAT GTTCGTGTGC GGCGTTAACC TGGACGCCTA CAGCCCCGAC 
ATGAAGGTGG TCTCCAACGC CTCGTGCACC ACCAACTGCC TGGCTCCCCT GGCCAAGGTC 
ATCAATGACA ACTTCGAGAT CGTCGAGGGT CTGATGACCA CCGTGCACGC CACCACTGCC 
ACCCAGAAGA CCGTCGACGG TCCCTCTGGC AAACTGTGGC GCGATGGACG TGGCGCCGCC 
CAGAACATCA TCCCGGCCGC CACCGGAGCC GCCAAGGCTG TGGGCAAGGT CATCCCCGCC 
CTGAACGGCA AGCTGACCGG CATGGCTTTC CGCGTGCCCA CGCCCAATGT CTCCGTTGTG 
GATCTTACCG TCCGCTTGGG CAAGGGAGCC ACCTATGACG AAATCAAGGC TAAGGTCGAG 
GAGGCCTCCA AGGGACCCCT GAAGGGAATC CTGGGCTACA CCGATGAGGA GGTGGTCTCC 
ACCGACTTCT TCAGCGACAC CCATTCGTCT GTGTTCGACG CCAAGGCTGG CATTTCGCTG 
AACGATAAGT TCGTCAAGCT AATCTCGTGG TACGACAACG AGTTCGGTTA CTCCAACCGC 
GTCATCGACC TGATCAAGTA TATGCAGAGC AAGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gapdh1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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