vari cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	vari
Entrez Gene ID	35343
Full Name	varicose
Synonyms	38E.21,CG9326,Dmel\CG9326,Vari,l(2)03953,l(2)03953b,l(2)38EFa,szar
General protein information	Preferred Names varicose Names CG9326 gene product from transcript CG9326-RD CG9326-PB CG9326-PC CG9326-PD CG9326-PE CG9326-PF lethal (2) 38EFa senz'aria senz-aria vari-PB vari-PC vari-PD vari-PE vari-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 38E1-39A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_165342.4	NP_724288.3	varicose, isoform B
NM_165343.3	NP_724289.2	varicose, isoform C
NM_001273715.1	NP_001260644.1	varicose, isoform E
NM_001273716.1	NP_001260645.1	varicose, isoform F
NM_206011.3	NP_995733.2	varicose, isoform D

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	MPP6	XP_002803397.1
Canis lupus familiaris (dog)	MPP6	XP_005628695.1
Rattus norvegicus (Norway rat)	Mpp6	NP_001128454.1
Danio rerio (zebrafish)	mpp6a	NP_001243118.1
Drosophila melanogaster (fruit fly)	vari	NP_001260644.1
Xenopus tropicalis (tropical clawed frog)	mpp6	NP_001017060.1
Homo sapiens (human)	MPP6	NP_057531.2
Pan troglodytes (chimpanzee)	MPP6	NP_001233585.1
Mus musculus (house mouse)	Mpp6	NP_001158205.1
Danio rerio (zebrafish)	mpp6b	NP_001038242.1
Bos taurus (cattle)	MPP6	XP_005205614.1
Gallus gallus (chicken)	MPP6	XP_418721.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008321	XP_317142.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following vari gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vari cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15063	NM_165343.3 Latest version!	Drosophila melanogaster varicose (vari), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa15063	NM_001273716.1 Latest version!	Drosophila melanogaster varicose (vari), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa15062	NM_206011.3 Latest version!	Drosophila melanogaster varicose (vari), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa15064	NM_165342.4 Latest version!	Drosophila melanogaster varicose (vari), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa16954	NM_001273715.1 Latest version!	Drosophila melanogaster varicose (vari), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vari ( NM_165343.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724289.2 Drosophila melanogaster varicose (vari), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa15063
Clone ID Related Accession (Same CDS sequence)	NM_165343.3 , NM_001273716.1
Accession Version	NM_165343.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	varicose, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165343.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGTGG AGACCATCAA GATGGTGGGT CTGCGCCGAG ATCCCAGCAA GCCGCTGGGC 
CTGACCGTCG AACTGGACGA ATTCAAGCAG CTGGTCGTGG CCAGGATTCT GGCGGGCGGG 
GTGATCGACA AACAGAGCAT GCTGCACGTT GGCGATGTCA TCCTAGAGGT GAACGGTACG 
CCCGTTCGCA CTCCCGATGA GCTGCAGGTG GAGGTGTCGC GGGCCAAGGA GAATCTCACC 
CTCAAGATCG GGCCGAACGT GGACGAGGAG ATCAAGAGCG GTCGCTATAC TGTGAGTGGG 
GGTCAGGTAA AACAGAATGG CATCGCGAGT CTCGAGACGG GCAAGAAACT GACGTGCTAC 
ATGCGTGCCC TTTTCACATA CAATCCATCC GAAGATTCCT TGCTGCCATG CAGGGATATT 
GGATTGCCCT TCAAGTCGGG CGACATTTTG CAGATCATCA ACGTAAAGGA TCCCAACTGG 
TGGCAGGCCA AGAACATTAC TGCCGAATCT GACAAAATTG GACTCATACC ATCCCAAGAG 
CTGGAGGAGC GGCGCAAAGC CTTCGTGGCC CCCGAGGCGG ACTACGTTCA CAAGATTGGC 
ATTTGCGGAA CAAGAATCTC GAAGCGAAAG CGCAAGACCA TGTACCGATC TGTGGCCAAT 
TGCGAGTTCG ACAAGGCGGA GCTGCTGCTC TATGAGGAGG TCACGCGGAT GCCGCCGTTC 
CGCAGGAAAA CCCTGGTTCT CATCGGTGTT TCCGGGGTGG GAAGACGTAC GCTCAAGAAT 
CGGCTGATCA ACAGCGATGT GGACAAGTTC GGAGCGGTCA TTCCACATAC CAGTCGCCCC 
AAGCGCGCCT TGGAGGAGAA CGGCAGTAGC TACTGGTTCA TGGACCGCGA GGAAATGGAA 
GAGGCCGTGC GGAACAACGA GTTCCTGGAG TACGGCGAGC ACAATGGCAA TCTGTACGGC 
ACGCATTTGC AGTCCATCAA GGATGTGATC AACAGTGGGC GCATGTGCAT CCTGGATTGT 
GCACCGAATG CCCTGAAGAT CCTGCACAAC AGCCAGGAAC TGATGCCTTT TGTCATCTTT 
GTGGCAGCGC CTGGCATGGA GCAGCTCAAG ACCATCTATG CGGATCGCAG GGCCACGGGC 
TCCAACCGGA ATTTATCTTT TGATCGCCAG AGTTCCATAA GATTCAGCTC CAGACGCGCC 
CGTACGCTCG AGTCCCTAGC GTCGTTGTAT GAGGACGACG ACCTTGTCGC CACCGTCGAG 
GAGAGCAGCT TCGTCCAGCG CAAGTATGAG AAATACTTCG ACATGGTCAT CGTGAACGAG 
GACTTCGATG AGACGTTCCG CCAGGTGGTG GAAACGCTGG ATCAGATGAG CCACGAGGAG 
CAGTGGGTGC CTGTCAACTG GATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724289.2
CDS	326..1735
Translation	MPVETIKMVGLRRDPSKPLGLTVELDEFKQLVVARILAGGVIDKQSMLHVGDVILEVNGTPVRTPDELQVEVSRAKENLTLKIGPNVDEEIKSGRYTVSGGQVKQNGIASLETGKKLTCYMRALFTYNPSEDSLLPCRDIGLPFKSGDILQIINVKDPNWWQAKNITAESDKIGLIPSQELEERRKAFVAPEADYVHKIGICGTRISKRKRKTMYRSVANCEFDKAELLLYEEVTRMPPFRRKTLVLIGVSGVGRRTLKNRLINSDVDKFGAVIPHTSRPKRALEENGSSYWFMDREEMEEAVRNNEFLEYGEHNGNLYGTHLQSIKDVINSGRMCILDCAPNALKILHNSQELMPFVIFVAAPGMEQLKTIYADRRATGSNRNLSFDRQSSIRFSSRRARTLESLASLYEDDDLVATVEESSFVQRKYEKYFDMVIVNEDFDETFRQVVETLDQMSHEEQWVPVNWIY

Target ORF information:

RefSeq Version	NM_165343.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster varicose (vari), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165343.3

ORF Insert Sequence:

ATGCCAGTGG AGACCATCAA GATGGTGGGT CTGCGCCGAG ATCCCAGCAA GCCGCTGGGC 
CTGACCGTCG AACTGGACGA ATTCAAGCAG CTGGTCGTGG CCAGGATTCT GGCGGGCGGG 
GTGATCGACA AACAGAGCAT GCTGCACGTT GGCGATGTCA TCCTAGAGGT GAACGGTACG 
CCCGTTCGCA CTCCCGATGA GCTGCAGGTG GAGGTGTCGC GGGCCAAGGA GAATCTCACC 
CTCAAGATCG GGCCGAACGT GGACGAGGAG ATCAAGAGCG GTCGCTATAC TGTGAGTGGG 
GGTCAGGTAA AACAGAATGG CATCGCGAGT CTCGAGACGG GCAAGAAACT GACGTGCTAC 
ATGCGTGCCC TTTTCACATA CAATCCATCC GAAGATTCCT TGCTGCCATG CAGGGATATT 
GGATTGCCCT TCAAGTCGGG CGACATTTTG CAGATCATCA ACGTAAAGGA TCCCAACTGG 
TGGCAGGCCA AGAACATTAC TGCCGAATCT GACAAAATTG GACTCATACC ATCCCAAGAG 
CTGGAGGAGC GGCGCAAAGC CTTCGTGGCC CCCGAGGCGG ACTACGTTCA CAAGATTGGC 
ATTTGCGGAA CAAGAATCTC GAAGCGAAAG CGCAAGACCA TGTACCGATC TGTGGCCAAT 
TGCGAGTTCG ACAAGGCGGA GCTGCTGCTC TATGAGGAGG TCACGCGGAT GCCGCCGTTC 
CGCAGGAAAA CCCTGGTTCT CATCGGTGTT TCCGGGGTGG GAAGACGTAC GCTCAAGAAT 
CGGCTGATCA ACAGCGATGT GGACAAGTTC GGAGCGGTCA TTCCACATAC CAGTCGCCCC 
AAGCGCGCCT TGGAGGAGAA CGGCAGTAGC TACTGGTTCA TGGACCGCGA GGAAATGGAA 
GAGGCCGTGC GGAACAACGA GTTCCTGGAG TACGGCGAGC ACAATGGCAA TCTGTACGGC 
ACGCATTTGC AGTCCATCAA GGATGTGATC AACAGTGGGC GCATGTGCAT CCTGGATTGT 
GCACCGAATG CCCTGAAGAT CCTGCACAAC AGCCAGGAAC TGATGCCTTT TGTCATCTTT 
GTGGCAGCGC CTGGCATGGA GCAGCTCAAG ACCATCTATG CGGATCGCAG GGCCACGGGC 
TCCAACCGGA ATTTATCTTT TGATCGCCAG AGTTCCATAA GATTCAGCTC CAGACGCGCC 
CGTACGCTCG AGTCCCTAGC GTCGTTGTAT GAGGACGACG ACCTTGTCGC CACCGTCGAG 
GAGAGCAGCT TCGTCCAGCG CAAGTATGAG AAATACTTCG ACATGGTCAT CGTGAACGAG 
GACTTCGATG AGACGTTCCG CCAGGTGGTG GAAACGCTGG ATCAGATGAG CCACGAGGAG 
CAGTGGGTGC CTGTCAACTG GATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vari ( NM_001273716.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260645.1 Drosophila melanogaster varicose (vari), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa15063
Clone ID Related Accession (Same CDS sequence)	NM_165343.3 , NM_001273716.1
Accession Version	NM_001273716.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	varicose, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGTGG AGACCATCAA GATGGTGGGT CTGCGCCGAG ATCCCAGCAA GCCGCTGGGC 
CTGACCGTCG AACTGGACGA ATTCAAGCAG CTGGTCGTGG CCAGGATTCT GGCGGGCGGG 
GTGATCGACA AACAGAGCAT GCTGCACGTT GGCGATGTCA TCCTAGAGGT GAACGGTACG 
CCCGTTCGCA CTCCCGATGA GCTGCAGGTG GAGGTGTCGC GGGCCAAGGA GAATCTCACC 
CTCAAGATCG GGCCGAACGT GGACGAGGAG ATCAAGAGCG GTCGCTATAC TGTGAGTGGG 
GGTCAGGTAA AACAGAATGG CATCGCGAGT CTCGAGACGG GCAAGAAACT GACGTGCTAC 
ATGCGTGCCC TTTTCACATA CAATCCATCC GAAGATTCCT TGCTGCCATG CAGGGATATT 
GGATTGCCCT TCAAGTCGGG CGACATTTTG CAGATCATCA ACGTAAAGGA TCCCAACTGG 
TGGCAGGCCA AGAACATTAC TGCCGAATCT GACAAAATTG GACTCATACC ATCCCAAGAG 
CTGGAGGAGC GGCGCAAAGC CTTCGTGGCC CCCGAGGCGG ACTACGTTCA CAAGATTGGC 
ATTTGCGGAA CAAGAATCTC GAAGCGAAAG CGCAAGACCA TGTACCGATC TGTGGCCAAT 
TGCGAGTTCG ACAAGGCGGA GCTGCTGCTC TATGAGGAGG TCACGCGGAT GCCGCCGTTC 
CGCAGGAAAA CCCTGGTTCT CATCGGTGTT TCCGGGGTGG GAAGACGTAC GCTCAAGAAT 
CGGCTGATCA ACAGCGATGT GGACAAGTTC GGAGCGGTCA TTCCACATAC CAGTCGCCCC 
AAGCGCGCCT TGGAGGAGAA CGGCAGTAGC TACTGGTTCA TGGACCGCGA GGAAATGGAA 
GAGGCCGTGC GGAACAACGA GTTCCTGGAG TACGGCGAGC ACAATGGCAA TCTGTACGGC 
ACGCATTTGC AGTCCATCAA GGATGTGATC AACAGTGGGC GCATGTGCAT CCTGGATTGT 
GCACCGAATG CCCTGAAGAT CCTGCACAAC AGCCAGGAAC TGATGCCTTT TGTCATCTTT 
GTGGCAGCGC CTGGCATGGA GCAGCTCAAG ACCATCTATG CGGATCGCAG GGCCACGGGC 
TCCAACCGGA ATTTATCTTT TGATCGCCAG AGTTCCATAA GATTCAGCTC CAGACGCGCC 
CGTACGCTCG AGTCCCTAGC GTCGTTGTAT GAGGACGACG ACCTTGTCGC CACCGTCGAG 
GAGAGCAGCT TCGTCCAGCG CAAGTATGAG AAATACTTCG ACATGGTCAT CGTGAACGAG 
GACTTCGATG AGACGTTCCG CCAGGTGGTG GAAACGCTGG ATCAGATGAG CCACGAGGAG 
CAGTGGGTGC CTGTCAACTG GATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260645.1
CDS	385..1794
Translation	MPVETIKMVGLRRDPSKPLGLTVELDEFKQLVVARILAGGVIDKQSMLHVGDVILEVNGTPVRTPDELQVEVSRAKENLTLKIGPNVDEEIKSGRYTVSGGQVKQNGIASLETGKKLTCYMRALFTYNPSEDSLLPCRDIGLPFKSGDILQIINVKDPNWWQAKNITAESDKIGLIPSQELEERRKAFVAPEADYVHKIGICGTRISKRKRKTMYRSVANCEFDKAELLLYEEVTRMPPFRRKTLVLIGVSGVGRRTLKNRLINSDVDKFGAVIPHTSRPKRALEENGSSYWFMDREEMEEAVRNNEFLEYGEHNGNLYGTHLQSIKDVINSGRMCILDCAPNALKILHNSQELMPFVIFVAAPGMEQLKTIYADRRATGSNRNLSFDRQSSIRFSSRRARTLESLASLYEDDDLVATVEESSFVQRKYEKYFDMVIVNEDFDETFRQVVETLDQMSHEEQWVPVNWIY

Target ORF information:

RefSeq Version	NM_001273716.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster varicose (vari), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273716.1

ORF Insert Sequence:

ATGCCAGTGG AGACCATCAA GATGGTGGGT CTGCGCCGAG ATCCCAGCAA GCCGCTGGGC 
CTGACCGTCG AACTGGACGA ATTCAAGCAG CTGGTCGTGG CCAGGATTCT GGCGGGCGGG 
GTGATCGACA AACAGAGCAT GCTGCACGTT GGCGATGTCA TCCTAGAGGT GAACGGTACG 
CCCGTTCGCA CTCCCGATGA GCTGCAGGTG GAGGTGTCGC GGGCCAAGGA GAATCTCACC 
CTCAAGATCG GGCCGAACGT GGACGAGGAG ATCAAGAGCG GTCGCTATAC TGTGAGTGGG 
GGTCAGGTAA AACAGAATGG CATCGCGAGT CTCGAGACGG GCAAGAAACT GACGTGCTAC 
ATGCGTGCCC TTTTCACATA CAATCCATCC GAAGATTCCT TGCTGCCATG CAGGGATATT 
GGATTGCCCT TCAAGTCGGG CGACATTTTG CAGATCATCA ACGTAAAGGA TCCCAACTGG 
TGGCAGGCCA AGAACATTAC TGCCGAATCT GACAAAATTG GACTCATACC ATCCCAAGAG 
CTGGAGGAGC GGCGCAAAGC CTTCGTGGCC CCCGAGGCGG ACTACGTTCA CAAGATTGGC 
ATTTGCGGAA CAAGAATCTC GAAGCGAAAG CGCAAGACCA TGTACCGATC TGTGGCCAAT 
TGCGAGTTCG ACAAGGCGGA GCTGCTGCTC TATGAGGAGG TCACGCGGAT GCCGCCGTTC 
CGCAGGAAAA CCCTGGTTCT CATCGGTGTT TCCGGGGTGG GAAGACGTAC GCTCAAGAAT 
CGGCTGATCA ACAGCGATGT GGACAAGTTC GGAGCGGTCA TTCCACATAC CAGTCGCCCC 
AAGCGCGCCT TGGAGGAGAA CGGCAGTAGC TACTGGTTCA TGGACCGCGA GGAAATGGAA 
GAGGCCGTGC GGAACAACGA GTTCCTGGAG TACGGCGAGC ACAATGGCAA TCTGTACGGC 
ACGCATTTGC AGTCCATCAA GGATGTGATC AACAGTGGGC GCATGTGCAT CCTGGATTGT 
GCACCGAATG CCCTGAAGAT CCTGCACAAC AGCCAGGAAC TGATGCCTTT TGTCATCTTT 
GTGGCAGCGC CTGGCATGGA GCAGCTCAAG ACCATCTATG CGGATCGCAG GGCCACGGGC 
TCCAACCGGA ATTTATCTTT TGATCGCCAG AGTTCCATAA GATTCAGCTC CAGACGCGCC 
CGTACGCTCG AGTCCCTAGC GTCGTTGTAT GAGGACGACG ACCTTGTCGC CACCGTCGAG 
GAGAGCAGCT TCGTCCAGCG CAAGTATGAG AAATACTTCG ACATGGTCAT CGTGAACGAG 
GACTTCGATG AGACGTTCCG CCAGGTGGTG GAAACGCTGG ATCAGATGAG CCACGAGGAG 
CAGTGGGTGC CTGTCAACTG GATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vari ( NM_206011.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995733.2 Drosophila melanogaster varicose (vari), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa15062
Clone ID Related Accession (Same CDS sequence)	NM_206011.3
Accession Version	NM_206011.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	varicose, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_206011.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTCGGT GGAGCGCGCG GTCGCGGCGC CAAGCTCGCC AGGATAAAGT GGAGCTATTG 
GCCAGAAACA ACAAAGTGAA TGGCGAGGAC GATACCTCCG ATAACGCCGC CTTTCGCAAC 
TCGACTGACC TATCCGACCA CGATGAGATC TTCCTCAAGG GATTGCTGCG TAGTAACTCC 
AACACGCCCC ACAAGGAACT GATGCTGAAT CCGACCGAGC CGCAGCCAGT GCCACTATTC 
CTGCCCGCTC ATCTGAACAA CAAGCCCATC TGCGACGACA TCATCCGCAA GTTCTCACCC 
TCGAGGCGCC TGGAGTCGCG AGAGCTCGCC AAGCTTCTCG CCCAGCCGCA TTTTCGTGCT 
CTTTTGCGCG CCCATGATGA GATAGGAGCA CTCTACGAGC AACGACTTAA AGCTGCCGGG 
GGATCCACCA GCCAACTAGA GATCGCCAGC CAACGCCAAA CGGGAGGCTA CCTGTTCACC 
GAGGACGTCC TCAACACCAA GATGCCAGTG GAGACCATCA AGATGGTGGG TCTGCGCCGA 
GATCCCAGCA AGCCGCTGGG CCTGACCGTC GAACTGGACG AATTCAAGCA GCTGGTCGTG 
GCCAGGATTC TGGCGGGCGG GGTGATCGAC AAACAGAGCA TGCTGCACGT TGGCGATGTC 
ATCCTAGAGG TGAACGGTAC GCCCGTTCGC ACTCCCGATG AGCTGCAGGT GGAGGTGTCG 
CGGGCCAAGG AGAATCTCAC CCTCAAGATC GGGCCGAACG TGGACGAGGA GATCAAGAGC 
GGTCGCTATA CTTGCTACAT GCGTGCCCTT TTCACATACA ATCCATCCGA AGATTCCTTG 
CTGCCATGCA GGGATATTGG ATTGCCCTTC AAGTCGGGCG ACATTTTGCA GATCATCAAC 
GTAAAGGATC CCAACTGGTG GCAGGCCAAG AACATTACTG CCGAATCTGA CAAAATTGGA 
CTCATACCAT CCCAAGAGCT GGAGGAGCGG CGCAAAGCCT TCGTGGCCCC CGAGGCGGAC 
TACGTTCACA AGATTGGCAT TTGCGGAACA AGAATCTCGA AGCGAAAGCG CAAGACCATG 
TACCGATCTG TGGCCAATTG CGAGTTCGAC AAGGCGGAGC TGCTGCTCTA TGAGGAGGTC 
ACGCGGATGC CGCCGTTCCG CAGGAAAACC CTGGTTCTCA TCGGTGTTTC CGGGGTGGGA 
AGACGTACGC TCAAGAATCG GCTGATCAAC AGCGATGTGG ACAAGTTCGG AGCGGTCATT 
CCACATACCA GTCGCCCCAA GCGCGCCTTG GAGGAGAACG GCAGTAGCTA CTGGTTCATG 
GACCGCGAGG AAATGGAAGA GGCCGTGCGG AACAACGAGT TCCTGGAGTA CGGCGAGCAC 
AATGGCAATC TGTACGGCAC GCATTTGCAG TCCATCAAGG ATGTGATCAA CAGTGGGCGC 
ATGTGCATCC TGGATTGTGC ACCGAATGCC CTGAAGATCC TGCACAACAG CCAGGAACTG 
ATGCCTTTTG TCATCTTTGT GGCAGCGCCT GGCATGGAGC AGCTCAAGAC CATCTATGCG 
GATCGCAGGG CCACGGGCTC CAACCGGAAT TTATCTTTTG ATCGCCAGAG TTCCATAAGA 
TTCAGCTCCA GACGCGCCCG TACGCTCGAG TCCCTAGCGT CGTTGTATGA GGACGACGAC 
CTTGTCGCCA CCGTCGAGGA GAGCAGCTTC GTCCAGCGCA AGTATGAGAA ATACTTCGAC 
ATGGTCATCG TGAACGAGGA CTTCGATGAG ACGTTCCGCC AGGTGGTGGA AACGCTGGAT 
CAGATGAGCC ACGAGGAGCA GTGGGTGCCT GTCAACTGGA TCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995733.2
CDS	285..2132
Translation	MVRWSARSRRQARQDKVELLARNNKVNGEDDTSDNAAFRNSTDLSDHDEIFLKGLLRSNSNTPHKELMLNPTEPQPVPLFLPAHLNNKPICDDIIRKFSPSRRLESRELAKLLAQPHFRALLRAHDEIGALYEQRLKAAGGSTSQLEIASQRQTGGYLFTEDVLNTKMPVETIKMVGLRRDPSKPLGLTVELDEFKQLVVARILAGGVIDKQSMLHVGDVILEVNGTPVRTPDELQVEVSRAKENLTLKIGPNVDEEIKSGRYTCYMRALFTYNPSEDSLLPCRDIGLPFKSGDILQIINVKDPNWWQAKNITAESDKIGLIPSQELEERRKAFVAPEADYVHKIGICGTRISKRKRKTMYRSVANCEFDKAELLLYEEVTRMPPFRRKTLVLIGVSGVGRRTLKNRLINSDVDKFGAVIPHTSRPKRALEENGSSYWFMDREEMEEAVRNNEFLEYGEHNGNLYGTHLQSIKDVINSGRMCILDCAPNALKILHNSQELMPFVIFVAAPGMEQLKTIYADRRATGSNRNLSFDRQSSIRFSSRRARTLESLASLYEDDDLVATVEESSFVQRKYEKYFDMVIVNEDFDETFRQVVETLDQMSHEEQWVPVNWIY

Target ORF information:

RefSeq Version	NM_206011.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster varicose (vari), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206011.3

ORF Insert Sequence:

ATGGTTCGGT GGAGCGCGCG GTCGCGGCGC CAAGCTCGCC AGGATAAAGT GGAGCTATTG 
GCCAGAAACA ACAAAGTGAA TGGCGAGGAC GATACCTCCG ATAACGCCGC CTTTCGCAAC 
TCGACTGACC TATCCGACCA CGATGAGATC TTCCTCAAGG GATTGCTGCG TAGTAACTCC 
AACACGCCCC ACAAGGAACT GATGCTGAAT CCGACCGAGC CGCAGCCAGT GCCACTATTC 
CTGCCCGCTC ATCTGAACAA CAAGCCCATC TGCGACGACA TCATCCGCAA GTTCTCACCC 
TCGAGGCGCC TGGAGTCGCG AGAGCTCGCC AAGCTTCTCG CCCAGCCGCA TTTTCGTGCT 
CTTTTGCGCG CCCATGATGA GATAGGAGCA CTCTACGAGC AACGACTTAA AGCTGCCGGG 
GGATCCACCA GCCAACTAGA GATCGCCAGC CAACGCCAAA CGGGAGGCTA CCTGTTCACC 
GAGGACGTCC TCAACACCAA GATGCCAGTG GAGACCATCA AGATGGTGGG TCTGCGCCGA 
GATCCCAGCA AGCCGCTGGG CCTGACCGTC GAACTGGACG AATTCAAGCA GCTGGTCGTG 
GCCAGGATTC TGGCGGGCGG GGTGATCGAC AAACAGAGCA TGCTGCACGT TGGCGATGTC 
ATCCTAGAGG TGAACGGTAC GCCCGTTCGC ACTCCCGATG AGCTGCAGGT GGAGGTGTCG 
CGGGCCAAGG AGAATCTCAC CCTCAAGATC GGGCCGAACG TGGACGAGGA GATCAAGAGC 
GGTCGCTATA CTTGCTACAT GCGTGCCCTT TTCACATACA ATCCATCCGA AGATTCCTTG 
CTGCCATGCA GGGATATTGG ATTGCCCTTC AAGTCGGGCG ACATTTTGCA GATCATCAAC 
GTAAAGGATC CCAACTGGTG GCAGGCCAAG AACATTACTG CCGAATCTGA CAAAATTGGA 
CTCATACCAT CCCAAGAGCT GGAGGAGCGG CGCAAAGCCT TCGTGGCCCC CGAGGCGGAC 
TACGTTCACA AGATTGGCAT TTGCGGAACA AGAATCTCGA AGCGAAAGCG CAAGACCATG 
TACCGATCTG TGGCCAATTG CGAGTTCGAC AAGGCGGAGC TGCTGCTCTA TGAGGAGGTC 
ACGCGGATGC CGCCGTTCCG CAGGAAAACC CTGGTTCTCA TCGGTGTTTC CGGGGTGGGA 
AGACGTACGC TCAAGAATCG GCTGATCAAC AGCGATGTGG ACAAGTTCGG AGCGGTCATT 
CCACATACCA GTCGCCCCAA GCGCGCCTTG GAGGAGAACG GCAGTAGCTA CTGGTTCATG 
GACCGCGAGG AAATGGAAGA GGCCGTGCGG AACAACGAGT TCCTGGAGTA CGGCGAGCAC 
AATGGCAATC TGTACGGCAC GCATTTGCAG TCCATCAAGG ATGTGATCAA CAGTGGGCGC 
ATGTGCATCC TGGATTGTGC ACCGAATGCC CTGAAGATCC TGCACAACAG CCAGGAACTG 
ATGCCTTTTG TCATCTTTGT GGCAGCGCCT GGCATGGAGC AGCTCAAGAC CATCTATGCG 
GATCGCAGGG CCACGGGCTC CAACCGGAAT TTATCTTTTG ATCGCCAGAG TTCCATAAGA 
TTCAGCTCCA GACGCGCCCG TACGCTCGAG TCCCTAGCGT CGTTGTATGA GGACGACGAC 
CTTGTCGCCA CCGTCGAGGA GAGCAGCTTC GTCCAGCGCA AGTATGAGAA ATACTTCGAC 
ATGGTCATCG TGAACGAGGA CTTCGATGAG ACGTTCCGCC AGGTGGTGGA AACGCTGGAT 
CAGATGAGCC ACGAGGAGCA GTGGGTGCCT GTCAACTGGA TCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vari ( NM_165342.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724288.3 Drosophila melanogaster varicose (vari), transcript variant B, mRNA.

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CloneID	OFa15064
Clone ID Related Accession (Same CDS sequence)	NM_165342.4
Accession Version	NM_165342.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	varicose, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165342.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTCGGT GGAGCGCGCG GTCGCGGCGC CAAGCTCGCC AGGATAAAGT GGAGCTATTG 
GCCAGAAACA ACAAAGTGAA TGGCGAGGAC GATACCTCCG ATAACGCCGC CTTTCGCAAC 
TCGACTGACC TATCCGACCA CGATGAGATC TTCCTCAAGG GATTGCTGCG TAGTAACTCC 
AACACGCCCC ACAAGGAACT GATGCTGAAT CCGACCGAGC CGCAGCCAGT GCCACTATTC 
CTGCCCGCTC ATCTGAACAA CAAGCCCATC TGCGACGACA TCATCCGCAA GTTCTCACCC 
TCGAGGCGCC TGGAGTCGCG AGAGCTCGCC AAGCTTCTCG CCCAGCCGCA TTTTCGTGCT 
CTTTTGCGCG CCCATGATGA GATAGGAGCA CTCTACGAGC AACGACTTAA AGCTGCCGGG 
GGATCCACCA GCCAACTAGA GATCGCCAGC CAACGCCAAA CGGGAGGCTA CCTGTTCACC 
GAGGACGTCC TCAACACCAA GATGCCAGTG GAGACCATCA AGATGGTGGG TCTGCGCCGA 
GATCCCAGCA AGCCGCTGGG CCTGACCGTC GAACTGGACG AATTCAAGCA GCTGGTCGTG 
GCCAGGATTC TGGCGGGCGG GGTGATCGAC AAACAGAGCA TGCTGCACGT TGGCGATGTC 
ATCCTAGAGG TGAACGGTAC GCCCGTTCGC ACTCCCGATG AGCTGCAGGT GGAGGTGTCG 
CGGGCCAAGG AGAATCTCAC CCTCAAGATC GGGCCGAACG TGGACGAGGA GATCAAGAGC 
GGTCGCTATA CTGTGAGTGG GGGTCAGGTA AAACAGAATG GCATCGCGAG TCTCGAGACG 
GGCAAGAAAC TGACGTGCTA CATGCGTGCC CTTTTCACAT ACAATCCATC CGAAGATTCC 
TTGCTGCCAT GCAGGGATAT TGGATTGCCC TTCAAGTCGG GCGACATTTT GCAGATCATC 
AACGTAAAGG ATCCCAACTG GTGGCAGGCC AAGAACATTA CTGCCGAATC TGACAAAATT 
GGACTCATAC CATCCCAAGA GCTGGAGGAG CGGCGCAAAG CCTTCGTGGC CCCCGAGGCG 
GACTACGTTC ACAAGATTGG CATTTGCGGA ACAAGAATCT CGAAGCGAAA GCGCAAGACC 
ATGTACCGAT CTGTGGCCAA TTGCGAGTTC GACAAGGCGG AGCTGCTGCT CTATGAGGAG 
GTCACGCGGA TGCCGCCGTT CCGCAGGAAA ACCCTGGTTC TCATCGGTGT TTCCGGGGTG 
GGAAGACGTA CGCTCAAGAA TCGGCTGATC AACAGCGATG TGGACAAGTT CGGAGCGGTC 
ATTCCACATA CCAGTCGCCC CAAGCGCGCC TTGGAGGAGA ACGGCAGTAG CTACTGGTTC 
ATGGACCGCG AGGAAATGGA AGAGGCCGTG CGGAACAACG AGTTCCTGGA GTACGGCGAG 
CACAATGGCA ATCTGTACGG CACGCATTTG CAGTCCATCA AGGATGTGAT CAACAGTGGG 
CGCATGTGCA TCCTGGATTG TGCACCGAAT GCCCTGAAGA TCCTGCACAA CAGCCAGGAA 
CTGATGCCTT TTGTCATCTT TGTGGCAGCG CCTGGCATGG AGCAGCTCAA GACCATCTAT 
GCGGATCGCA GGGCCACGGG CTCCAACCGG AATTTATCTT TTGATCGCCA GAGTTCCATA 
AGATTCAGCT CCAGACGCGC CCGTACGCTC GAGTCCCTAG CGTCGTTGTA TGAGGACGAC 
GACCTTGTCG CCACCGTCGA GGAGAGCAGC TTCGTCCAGC GCAAGTATGA GAAATACTTC 
GACATGGTCA TCGTGAACGA GGACTTCGAT GAGACGTTCC GCCAGGTGGT GGAAACGCTG 
GATCAGATGA GCCACGAGGA GCAGTGGGTG CCTGTCAACT GGATCTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724288.3
CDS	285..2195
Translation	MVRWSARSRRQARQDKVELLARNNKVNGEDDTSDNAAFRNSTDLSDHDEIFLKGLLRSNSNTPHKELMLNPTEPQPVPLFLPAHLNNKPICDDIIRKFSPSRRLESRELAKLLAQPHFRALLRAHDEIGALYEQRLKAAGGSTSQLEIASQRQTGGYLFTEDVLNTKMPVETIKMVGLRRDPSKPLGLTVELDEFKQLVVARILAGGVIDKQSMLHVGDVILEVNGTPVRTPDELQVEVSRAKENLTLKIGPNVDEEIKSGRYTVSGGQVKQNGIASLETGKKLTCYMRALFTYNPSEDSLLPCRDIGLPFKSGDILQIINVKDPNWWQAKNITAESDKIGLIPSQELEERRKAFVAPEADYVHKIGICGTRISKRKRKTMYRSVANCEFDKAELLLYEEVTRMPPFRRKTLVLIGVSGVGRRTLKNRLINSDVDKFGAVIPHTSRPKRALEENGSSYWFMDREEMEEAVRNNEFLEYGEHNGNLYGTHLQSIKDVINSGRMCILDCAPNALKILHNSQELMPFVIFVAAPGMEQLKTIYADRRATGSNRNLSFDRQSSIRFSSRRARTLESLASLYEDDDLVATVEESSFVQRKYEKYFDMVIVNEDFDETFRQVVETLDQMSHEEQWVPVNWIY

Target ORF information:

RefSeq Version	NM_165342.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster varicose (vari), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165342.4

ORF Insert Sequence:

ATGGTTCGGT GGAGCGCGCG GTCGCGGCGC CAAGCTCGCC AGGATAAAGT GGAGCTATTG 
GCCAGAAACA ACAAAGTGAA TGGCGAGGAC GATACCTCCG ATAACGCCGC CTTTCGCAAC 
TCGACTGACC TATCCGACCA CGATGAGATC TTCCTCAAGG GATTGCTGCG TAGTAACTCC 
AACACGCCCC ACAAGGAACT GATGCTGAAT CCGACCGAGC CGCAGCCAGT GCCACTATTC 
CTGCCCGCTC ATCTGAACAA CAAGCCCATC TGCGACGACA TCATCCGCAA GTTCTCACCC 
TCGAGGCGCC TGGAGTCGCG AGAGCTCGCC AAGCTTCTCG CCCAGCCGCA TTTTCGTGCT 
CTTTTGCGCG CCCATGATGA GATAGGAGCA CTCTACGAGC AACGACTTAA AGCTGCCGGG 
GGATCCACCA GCCAACTAGA GATCGCCAGC CAACGCCAAA CGGGAGGCTA CCTGTTCACC 
GAGGACGTCC TCAACACCAA GATGCCAGTG GAGACCATCA AGATGGTGGG TCTGCGCCGA 
GATCCCAGCA AGCCGCTGGG CCTGACCGTC GAACTGGACG AATTCAAGCA GCTGGTCGTG 
GCCAGGATTC TGGCGGGCGG GGTGATCGAC AAACAGAGCA TGCTGCACGT TGGCGATGTC 
ATCCTAGAGG TGAACGGTAC GCCCGTTCGC ACTCCCGATG AGCTGCAGGT GGAGGTGTCG 
CGGGCCAAGG AGAATCTCAC CCTCAAGATC GGGCCGAACG TGGACGAGGA GATCAAGAGC 
GGTCGCTATA CTGTGAGTGG GGGTCAGGTA AAACAGAATG GCATCGCGAG TCTCGAGACG 
GGCAAGAAAC TGACGTGCTA CATGCGTGCC CTTTTCACAT ACAATCCATC CGAAGATTCC 
TTGCTGCCAT GCAGGGATAT TGGATTGCCC TTCAAGTCGG GCGACATTTT GCAGATCATC 
AACGTAAAGG ATCCCAACTG GTGGCAGGCC AAGAACATTA CTGCCGAATC TGACAAAATT 
GGACTCATAC CATCCCAAGA GCTGGAGGAG CGGCGCAAAG CCTTCGTGGC CCCCGAGGCG 
GACTACGTTC ACAAGATTGG CATTTGCGGA ACAAGAATCT CGAAGCGAAA GCGCAAGACC 
ATGTACCGAT CTGTGGCCAA TTGCGAGTTC GACAAGGCGG AGCTGCTGCT CTATGAGGAG 
GTCACGCGGA TGCCGCCGTT CCGCAGGAAA ACCCTGGTTC TCATCGGTGT TTCCGGGGTG 
GGAAGACGTA CGCTCAAGAA TCGGCTGATC AACAGCGATG TGGACAAGTT CGGAGCGGTC 
ATTCCACATA CCAGTCGCCC CAAGCGCGCC TTGGAGGAGA ACGGCAGTAG CTACTGGTTC 
ATGGACCGCG AGGAAATGGA AGAGGCCGTG CGGAACAACG AGTTCCTGGA GTACGGCGAG 
CACAATGGCA ATCTGTACGG CACGCATTTG CAGTCCATCA AGGATGTGAT CAACAGTGGG 
CGCATGTGCA TCCTGGATTG TGCACCGAAT GCCCTGAAGA TCCTGCACAA CAGCCAGGAA 
CTGATGCCTT TTGTCATCTT TGTGGCAGCG CCTGGCATGG AGCAGCTCAA GACCATCTAT 
GCGGATCGCA GGGCCACGGG CTCCAACCGG AATTTATCTT TTGATCGCCA GAGTTCCATA 
AGATTCAGCT CCAGACGCGC CCGTACGCTC GAGTCCCTAG CGTCGTTGTA TGAGGACGAC 
GACCTTGTCG CCACCGTCGA GGAGAGCAGC TTCGTCCAGC GCAAGTATGA GAAATACTTC 
GACATGGTCA TCGTGAACGA GGACTTCGAT GAGACGTTCC GCCAGGTGGT GGAAACGCTG 
GATCAGATGA GCCACGAGGA GCAGTGGGTG CCTGTCAACT GGATCTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vari ( NM_001273715.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260644.1 Drosophila melanogaster varicose (vari), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa16954
Clone ID Related Accession (Same CDS sequence)	NM_001273715.1
Accession Version	NM_001273715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	varicose, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTCGGT GGAGCGCGCG GTCGCGGCGC CAAGCTCGCC AGGATAAAGT GGAGCTATTG 
GCCAGAAACA ACAAAGTGAA TGGCGAGGAC GATACCTCCG ATAACGCCGC CTTTCGCAAC 
TCGACTGACC TATCCGACCA CGATGAGATC TTCCTCAAGG GATTGCTGCG TAGTAACTCC 
AACACGCCCC ACAAGGAACT GATGCTGAAT CCGACCGAGC CGCAGCCAGT GCCACTATTC 
CTGCCCGCTC ATCTGAACAA CAAGCCCATC TGCGACGACA TCATCCGCAA GTTCTCACCC 
TCGAGGCGCC TGGAGTCGCG AGAGCTCGCC AAGCTTCTCG CCCAGCCGCA TTTTCGTGCT 
CTTTTGCGCG CCCATGATGA GATAGGAGCA CTCTACGAGC AACGACTTAA AGCTGCCGGG 
GGATCCACCA GCCAACTAGA GATCGCCAGC CAACGCCAAA CGGGAGGCTA CCTGTTCACC 
GAGGACGTCC TCAACACCAA GATGCCAGTG GAGACCATCA AGATGGTGGG TCTGCGCCGA 
GATCCCAGCA AGCCGCTGGG CCTGACCGTC GAACTGGACG AATTCAAGCA GCTGGTCGTG 
GCCAGGATTC TGGCGGGCGG GGTGATCGAC AAACAGAGCA TGCTGCACGT TGGCGATGTC 
ATCCTAGAGG TGAACGGTAC GCCCGTTCGC ACTCCCGATG AGCTGCAGGT GGAGGTGTCG 
CGGGCCAAGG AGAATCTCAC CCTCAAGATC GGGCCGAACG TGGACGAGGA GATCAAGAGC 
GGTCGCTATA CTGTGAGTGG GGGTCAGGTA AAACAGAATG GCATCGCGAG TCTCGAGACG 
GGCAAGAAAC TGACGTGCTA CATGCGTGCC CTTTTCACAT ACAATCCATC CGAAGATTCC 
TTGCTGCCAT GCAGGGATAT TGGATTGCCC TTCAAGTCGG GCGACATTTT GCAGATCATC 
AACGTAAAGG ATCCCAACTG GTGGCAGGCC AAGAACATTA CTGCCGAATC TGACAAAATT 
GGACTCATAC CATCCCAAGA GCTGGAGGAG CGGCGCAAAG CCTTCGTGGC CCCCGAGGCG 
GACTACGTTC ACAAGATTGG CATTTGCGGA ACAAGAATCT CGAAGCGAAA GCGCAAGACC 
ATGTACCGAT CTGTGGCCAA TTGCGAGTTC GACAAGGCGG AGCTGCTGCT CTATGAGGAG 
GTCACGCGGA TGCCGCCGTT CCGCAGGAAA ACCCTGGTTC TCATCGGTGT TTCCGGGGTG 
GGAAGACGTA CGCTCAAGAA TCGGCTGATC AACAGCGATG TGGACAAGTT CGGAGCGGTC 
ATTCCACATA CCAGTCGCCC CAAGCGCGCC TTGGAGGAGA ACGGCAGTAG CTACTGGTTC 
ATGGACCGCG AGGAAATGGA AGAGGCCGTG CGGAACAACG AGTTCCTGGA GTACGGCGAG 
CACAATGGCA ATCTGTACGG CACGCATTTG CAGTCCATCA AGGATGTGAT CAACAGTGGG 
CGCATGTGCA TCCTGGATTG TGCACCGAAT GCCCTGAAGA TCCTGCACAA CAGCCAGGAA 
CTGATGCCTT TTGTCATCTT TGTGGCAGCG CCTGGCATGG AGCAGCTCAA GACCATCTAT 
GCGGATCGCA GGGCCACGGG CTCCAACCGG AATTTATCTG ACGACGACCT TGTCGCCACC 
GTCGAGGAGA GCAGCTTCGT CCAGCGCAAG TATGAGAAAT ACTTCGACAT GGTCATCGTG 
AACGAGGACT TCGATGAGAC GTTCCGCCAG GTGGTGGAAA CGCTGGATCA GATGAGCCAC 
GAGGAGCAGT GGGTGCCTGT CAACTGGATC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260644.1
CDS	285..2120
Translation	MVRWSARSRRQARQDKVELLARNNKVNGEDDTSDNAAFRNSTDLSDHDEIFLKGLLRSNSNTPHKELMLNPTEPQPVPLFLPAHLNNKPICDDIIRKFSPSRRLESRELAKLLAQPHFRALLRAHDEIGALYEQRLKAAGGSTSQLEIASQRQTGGYLFTEDVLNTKMPVETIKMVGLRRDPSKPLGLTVELDEFKQLVVARILAGGVIDKQSMLHVGDVILEVNGTPVRTPDELQVEVSRAKENLTLKIGPNVDEEIKSGRYTVSGGQVKQNGIASLETGKKLTCYMRALFTYNPSEDSLLPCRDIGLPFKSGDILQIINVKDPNWWQAKNITAESDKIGLIPSQELEERRKAFVAPEADYVHKIGICGTRISKRKRKTMYRSVANCEFDKAELLLYEEVTRMPPFRRKTLVLIGVSGVGRRTLKNRLINSDVDKFGAVIPHTSRPKRALEENGSSYWFMDREEMEEAVRNNEFLEYGEHNGNLYGTHLQSIKDVINSGRMCILDCAPNALKILHNSQELMPFVIFVAAPGMEQLKTIYADRRATGSNRNLSDDDLVATVEESSFVQRKYEKYFDMVIVNEDFDETFRQVVETLDQMSHEEQWVPVNWIY

Target ORF information:

RefSeq Version	NM_001273715.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster varicose (vari), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273715.1

ORF Insert Sequence:

ATGGTTCGGT GGAGCGCGCG GTCGCGGCGC CAAGCTCGCC AGGATAAAGT GGAGCTATTG 
GCCAGAAACA ACAAAGTGAA TGGCGAGGAC GATACCTCCG ATAACGCCGC CTTTCGCAAC 
TCGACTGACC TATCCGACCA CGATGAGATC TTCCTCAAGG GATTGCTGCG TAGTAACTCC 
AACACGCCCC ACAAGGAACT GATGCTGAAT CCGACCGAGC CGCAGCCAGT GCCACTATTC 
CTGCCCGCTC ATCTGAACAA CAAGCCCATC TGCGACGACA TCATCCGCAA GTTCTCACCC 
TCGAGGCGCC TGGAGTCGCG AGAGCTCGCC AAGCTTCTCG CCCAGCCGCA TTTTCGTGCT 
CTTTTGCGCG CCCATGATGA GATAGGAGCA CTCTACGAGC AACGACTTAA AGCTGCCGGG 
GGATCCACCA GCCAACTAGA GATCGCCAGC CAACGCCAAA CGGGAGGCTA CCTGTTCACC 
GAGGACGTCC TCAACACCAA GATGCCAGTG GAGACCATCA AGATGGTGGG TCTGCGCCGA 
GATCCCAGCA AGCCGCTGGG CCTGACCGTC GAACTGGACG AATTCAAGCA GCTGGTCGTG 
GCCAGGATTC TGGCGGGCGG GGTGATCGAC AAACAGAGCA TGCTGCACGT TGGCGATGTC 
ATCCTAGAGG TGAACGGTAC GCCCGTTCGC ACTCCCGATG AGCTGCAGGT GGAGGTGTCG 
CGGGCCAAGG AGAATCTCAC CCTCAAGATC GGGCCGAACG TGGACGAGGA GATCAAGAGC 
GGTCGCTATA CTGTGAGTGG GGGTCAGGTA AAACAGAATG GCATCGCGAG TCTCGAGACG 
GGCAAGAAAC TGACGTGCTA CATGCGTGCC CTTTTCACAT ACAATCCATC CGAAGATTCC 
TTGCTGCCAT GCAGGGATAT TGGATTGCCC TTCAAGTCGG GCGACATTTT GCAGATCATC 
AACGTAAAGG ATCCCAACTG GTGGCAGGCC AAGAACATTA CTGCCGAATC TGACAAAATT 
GGACTCATAC CATCCCAAGA GCTGGAGGAG CGGCGCAAAG CCTTCGTGGC CCCCGAGGCG 
GACTACGTTC ACAAGATTGG CATTTGCGGA ACAAGAATCT CGAAGCGAAA GCGCAAGACC 
ATGTACCGAT CTGTGGCCAA TTGCGAGTTC GACAAGGCGG AGCTGCTGCT CTATGAGGAG 
GTCACGCGGA TGCCGCCGTT CCGCAGGAAA ACCCTGGTTC TCATCGGTGT TTCCGGGGTG 
GGAAGACGTA CGCTCAAGAA TCGGCTGATC AACAGCGATG TGGACAAGTT CGGAGCGGTC 
ATTCCACATA CCAGTCGCCC CAAGCGCGCC TTGGAGGAGA ACGGCAGTAG CTACTGGTTC 
ATGGACCGCG AGGAAATGGA AGAGGCCGTG CGGAACAACG AGTTCCTGGA GTACGGCGAG 
CACAATGGCA ATCTGTACGG CACGCATTTG CAGTCCATCA AGGATGTGAT CAACAGTGGG 
CGCATGTGCA TCCTGGATTG TGCACCGAAT GCCCTGAAGA TCCTGCACAA CAGCCAGGAA 
CTGATGCCTT TTGTCATCTT TGTGGCAGCG CCTGGCATGG AGCAGCTCAA GACCATCTAT 
GCGGATCGCA GGGCCACGGG CTCCAACCGG AATTTATCTG ACGACGACCT TGTCGCCACC 
GTCGAGGAGA GCAGCTTCGT CCAGCGCAAG TATGAGAAAT ACTTCGACAT GGTCATCGTG 
AACGAGGACT TCGATGAGAC GTTCCGCCAG GTGGTGGAAA CGCTGGATCA GATGAGCCAC 
GAGGAGCAGT GGGTGCCTGT CAACTGGATC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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vari cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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