brun cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	brun
Entrez Gene ID	35325
Full Name	brunelleschi
Synonyms	38D.28,CG2478,Dmel\CG2478,bru
General protein information	Preferred Names brunelleschi Names CG2478 gene product from transcript CG2478-RA CG2478-PA brun-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 38D2-38D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_136200.3	NP_610044.2	brunelleschi

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRAPPC9	NP_113654.4
Pan troglodytes (chimpanzee)	TRAPPC9	XP_001142596.1
Mus musculus (house mouse)	Trappc9	NP_850993.2
Drosophila melanogaster (fruit fly)	bru	NP_610044.2
Xenopus tropicalis (tropical clawed frog)	trappc9	NP_001135612.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009487	XP_310210.4
Bos taurus (cattle)	TRAPPC9	NP_001032551.1
Danio rerio (zebrafish)	LOC100002630	XP_003200576.1
Canis lupus familiaris (dog)	TRAPPC9	XP_539179.2
Rattus norvegicus (Norway rat)	Trappc9	NP_001029328.1
Gallus gallus (chicken)	TRAPPC9	XP_004940119.1
Caenorhabditis elegans (roundworm)	C35C5.6	NP_509933.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following brun gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brun cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15045	NM_136200.3 Latest version!	Drosophila melanogaster brunelleschi (brun), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

brun ( NM_136200.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610044.2 Drosophila melanogaster brunelleschi (brun), mRNA.

Price & Availability↑

CloneID	OFa15045
Clone ID Related Accession (Same CDS sequence)	NM_136200.3
Accession Version	NM_136200.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	brunelleschi
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_136200.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTGCGG CCGTTGGCCT GATGCTGTCG CACGGTGCTG GTGGCATGGA GCCAGCGTTG 
TCACGCCCAG ACTACGAGCA GAGCGCCCTG CACCACAGCT GCCTGCTGGT GCTGCTCCGC 
GGCGTGGGTC CATCGCGCGC TCGCGTCCTT CAGCGGGCGT TTGAGAAAGT GCGACGTGTC 
AATCACATCC GGGTCAATGA CTCCTCTGGA CATCCCCGCT CTATTTGGAT CCGCTTCGTT 
CACGATCATC CTGTGGAGCA CAACGATTGG GGCGACTTTC AGACCCATCG CCGACTGCTG 
GGACTTGTCA CCATTGGCAA ATTCGATAGC CAAATCGAGC TCAATGAGCT GTGCCGTCAG 
CACGAGTCCT TAAAAGTTCG CTACGGGTCT ACTCTGTACG AGTCCCGTGC AATTTTCTTT 
GGGCCCGATG AGCAGCCACT GGAAACCATT GGGGAGGTGC TGGGACCACC GGCAGCTGGT 
GGACGACGCC TTCAAGATGA ATTCACAACG CCATCGAACT TCAAAGCGCA GGCCTTTTTC 
TATCGCGAAC AGGATTCGTG TGCCGATCTC GAATCTCGAA TTGGGGACTT TGCTAGCGCG 
TTGTTTTGGG TCCTGGAATC ACGACGGTTA GAGCGATCTC GCGAGAAGGC GGACAAGGTG 
AGCCTGTTGC TGGCACCCTT TGAAAAGCGA GACTTTGTGG GCCTGGACAT GGAGTCGAGG 
AACAATAGGA AAAGATGCGT GGGTCGTGTG ATGAAGAACC TGGCAGATCT ATCGCTGCAA 
GCTGGCCTGG TTGACGACGC CTTGAGTCTG TACCACAATG CCAATGAGAC TTTGAGGTCG 
GTAGGTGATT CCCTTTGGGT TGGGGCAACC GAAGAGGGCT TGTGTGCCGC TTCCGCAATG 
CTGTTGTATC CCCAAATGCG AGAAACCGAA ACGCTGCATA GAAACTCGTC GCTTCAGGAG 
GCGGGCACCT CTCCCCTAAA AAACACCCCT GAAAAGTGGC GTGCTAGCGA CGCCACCAAG 
AAGATTAGCG CCAGTGACGC GACCGCGAAC AATGTGGATT CCAACCAGCC TCAGCAGCGC 
GTAACCTCGA ACTCATCTTC CTGCTCATCC GTGTCTTCCC TGGTGACAAC GGCCACCAAT 
TCCTCCGCCT CGGACACGCC AACTACATCA TCTTCCTCCA CTTCGACGAT ATCGGCAGCA 
CCGATACCAG GACACCAGAG GAACGGCGAT CTGCCGGGCA ACATCCTCAA GGCGGAGGAG 
ATCAGCAACT ACTATCGCAA GGCCATCATT AACTACAGCA AGTACAGGCA TGCAGCCACT 
ATAGAGACGG AGGCGGCACT GAAGGCCTCG CGGATTTGCA TCGAGCAGAA TCGTCCCCTG 
GACGTGGCCA TGTTTTTGCA GAATATACTC TACATAAACC TGAGCATGAG CGAAGCCGAG 
CGGGTAAAGA GATTCGAGGT CATTACGGAT CTTTACCAGC AGATCGGATA CCAAAGGAAG 
GCGGCATTTT TCCAAAGACT CGCTGCACTC AAGCACGTTC AGCAGGGCAG CCAAGCGCCG 
GACTGGAACC AAAGTTACCG CCTCATGTTG GGCAGTTTTA CGGGGTACCG GCTTTGTCTC 
GACCCCTTGG AAGTAATAGA AAATGCCGCC GGCTGGCCGG CGTTGCAAAT CGATCTGGTC 
CAGACCCTTA TAACTGCCGC CAGGCGTTTG GGACACTCCG CTTTGGCCAC GCGGCACATG 
ACCTTTTTGC TGCAGACTCA GTGGGACAAC ATGTCGCCAA CGGAGCAAAG CGAGATGGCT 
GTGCAACTGC AGAATTTAAG CGCTCAGTGC GAGGGCTCTC CTGTGCCCTT GGTGCTGGAA 
AATGGCACTG TGATACCACC CGCCAATCTC ACGGATCTGC CCTACTGTAT CGATCTTCAG 
GTTAAAGATT TGCCCGCTCA TTTGCGACCG CAAAGAATAA AGGTGGCCAA AGCGGACAGT 
GGGCCTTTCC TATTCACCCC CATACACTTT AATTCTGTCG ACAGGAGGGA TAAGAAAAAG 
GACAAAAATA AAATAGCTTT CCAGTGGGTG CAAAATGATC TTAGTGAAGT GACTGTGCGC 
CTGCGGAATC CCCTGCCCTT TGAGCTGCCC GTAACGGACA TGAGATTGCT AACAAACGGC 
GTGGTGTTTG AGTCTTTGCC CCAGACTCTT GTACTACAGC CACATGTGCC CACGTATGTG 
GCGCTACATG GAACGCCAAT TGAGACGGGG CAACTCGATC TGCAGGGCTA CAGCACGCAC 
ACGCTCGGTG TGAAGTCCAA CTGTCGGCTG AAGCACATGC GTGGTCGTAG CTTTCCGCCC 
AACTACGTGG TTGATGTTAT CCCCGCCCTT CCTAGGATAT CTGTGAAAAC TTCGCTACCA 
CAGACGGCGA CGTTTAGCAA CATGAACAGT GCGGATATCG TTGTGACCTC TGCCAGCTTA 
ACGCTGTACA ACGGAGAATC CTCCAGCTGC ACAATAACTA TTACCAATGA GAGCGCCACG 
CTGCCACTGG AGCACCTAGA GTTTAGCATC AACTCAAATG TGGAGCAGGA GCTGCAGCAG 
AAGATCTTCC GAATAGATGA AGAAGCCATC AAGGCCCATT TGCCAGTGCC TCCCCAGGGC 
ACCATTGAAA TCATAGTGGA TGTTTTTGCC GAAGCGGACT TCGTTTGTCC TCAACCACCT 
GCTTCATTGC ATTCTGCCGC TGCTCCTGGG GACTACGGTG CGTCGTCACT AACGCACTAC 
TCGAGCGTGT CCACCTCTGG GCACGCCAGC TTACCCTCGA GAGTCGGTTC ACCGCATCAT 
CGTCGAAATG AGCCACAGAA TTCCAGTTTC CGGTCGACCA TTTCTGGCGG TCCACCATCG 
CTGGCTGCCT TAACCCTGCA CCCTGGCGGC GGTGGAGGAG TAGGACCTTC CAGCCTTGGG 
TCACAATATA ACCAGCACAT AGAGGCGCAA GTTAGATTTA AGTACTCGGG CGGAGATGCT 
TTGACGGCGG GCTACTGTCG CCAATGTGCG GTGTCCTTTA ATCTAGAACT TTTGCCTAGC 
GTACAGATTA CAAGCTGGGA TGTGCTTCCC GCAGAGGTAG CCTCCCAATT TTACCTGGTA 
CTTGACATCT CCAACCTAAC CGCTCAAGAG ATGTCGCTCA ACTATACGGA CACCAAGAAT 
ATACTCATCG AGGCCAAAGA GAGCTGCCGA GTGCCCATAC CCGTGGATCG GTGCTCCTTG 
GAGAAAGTGG TGGCTGCTCG GGCGGCCGAG GTAGCTGAGA ATCTGGAGAG GGAACTCTGC 
TTCCGGACGC AGCTGCTGTC GTTTAACGAT GCTCTGAGTA AGCTCTGCTC AATTCATATA 
GCTGAAAGGG TGAAAATCAA GTGGCTGTTA ACGGGGACGG ACATTCAGGG AATCGCCTCA 
CTGCGAGGAA TAGTTCTCTC CCAGTCTATG GTGGATTTGA CAGCTGTGTC GCCTTTGGAG 
TGGGCTATCA GCTTTCAAGA CACGCTCGTG CAGCCACACA ATGAGATTGT ATGCACGGTG 
GGACAGCGGT CGCTGCTAAG CATCCAGCTG GCCAATCAAT CCCTGCAACC ACTTAGAAAT 
CTGGTGCTGA GCATTAAGTT CTATCAGGAT TACCTGAACG GAATGGAAAA TTACAATTTG 
GAGACACGCG TGGCCATTTC AGGACCAAAT AGAATTGCAA TACCACTACT GGAGAAGCAG 
GAGCAGAAAG AGCATACTTG CTCTGTGATA TTCTTTACCC CCGGACGTTT CAAGGCCAGC 
ATCGAATGCA CCAGCAATCC GCAGAAGCAG TCGGAGCAGC CGTCGTCATT GCTAACCCGC 
TCCTGTCCGG CAGAAGCGGA GAGTGTCGGC CAATCGGTCA TGTTCTCCTC CAGCTACGAT 
GAGCAGCAGG CGCATGTGTG GAAGTTCATT CCGCCCATCG AGGTGACCGT CGTGGAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610044.2
CDS	395..4357
Translation	MRAAVGLMLSHGAGGMEPALSRPDYEQSALHHSCLLVLLRGVGPSRARVLQRAFEKVRRVNHIRVNDSSGHPRSIWIRFVHDHPVEHNDWGDFQTHRRLLGLVTIGKFDSQIELNELCRQHESLKVRYGSTLYESRAIFFGPDEQPLETIGEVLGPPAAGGRRLQDEFTTPSNFKAQAFFYREQDSCADLESRIGDFASALFWVLESRRLERSREKADKVSLLLAPFEKRDFVGLDMESRNNRKRCVGRVMKNLADLSLQAGLVDDALSLYHNANETLRSVGDSLWVGATEEGLCAASAMLLYPQMRETETLHRNSSLQEAGTSPLKNTPEKWRASDATKKISASDATANNVDSNQPQQRVTSNSSSCSSVSSLVTTATNSSASDTPTTSSSSTSTISAAPIPGHQRNGDLPGNILKAEEISNYYRKAIINYSKYRHAATIETEAALKASRICIEQNRPLDVAMFLQNILYINLSMSEAERVKRFEVITDLYQQIGYQRKAAFFQRLAALKHVQQGSQAPDWNQSYRLMLGSFTGYRLCLDPLEVIENAAGWPALQIDLVQTLITAARRLGHSALATRHMTFLLQTQWDNMSPTEQSEMAVQLQNLSAQCEGSPVPLVLENGTVIPPANLTDLPYCIDLQVKDLPAHLRPQRIKVAKADSGPFLFTPIHFNSVDRRDKKKDKNKIAFQWVQNDLSEVTVRLRNPLPFELPVTDMRLLTNGVVFESLPQTLVLQPHVPTYVALHGTPIETGQLDLQGYSTHTLGVKSNCRLKHMRGRSFPPNYVVDVIPALPRISVKTSLPQTATFSNMNSADIVVTSASLTLYNGESSSCTITITNESATLPLEHLEFSINSNVEQELQQKIFRIDEEAIKAHLPVPPQGTIEIIVDVFAEADFVCPQPPASLHSAAAPGDYGASSLTHYSSVSTSGHASLPSRVGSPHHRRNEPQNSSFRSTISGGPPSLAALTLHPGGGGGVGPSSLGSQYNQHIEAQVRFKYSGGDALTAGYCRQCAVSFNLELLPSVQITSWDVLPAEVASQFYLVLDISNLTAQEMSLNYTDTKNILIEAKESCRVPIPVDRCSLEKVVAARAAEVAENLERELCFRTQLLSFNDALSKLCSIHIAERVKIKWLLTGTDIQGIASLRGIVLSQSMVDLTAVSPLEWAISFQDTLVQPHNEIVCTVGQRSLLSIQLANQSLQPLRNLVLSIKFYQDYLNGMENYNLETRVAISGPNRIAIPLLEKQEQKEHTCSVIFFTPGRFKASIECTSNPQKQSEQPSSLLTRSCPAEAESVGQSVMFSSSYDEQQAHVWKFIPPIEVTVVEQ

Target ORF information:

RefSeq Version	NM_136200.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brunelleschi (brun), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136200.3

ORF Insert Sequence:

ATGCGTGCGG CCGTTGGCCT GATGCTGTCG CACGGTGCTG GTGGCATGGA GCCAGCGTTG 
TCACGCCCAG ACTACGAGCA GAGCGCCCTG CACCACAGCT GCCTGCTGGT GCTGCTCCGC 
GGCGTGGGTC CATCGCGCGC TCGCGTCCTT CAGCGGGCGT TTGAGAAAGT GCGACGTGTC 
AATCACATCC GGGTCAATGA CTCCTCTGGA CATCCCCGCT CTATTTGGAT CCGCTTCGTT 
CACGATCATC CTGTGGAGCA CAACGATTGG GGCGACTTTC AGACCCATCG CCGACTGCTG 
GGACTTGTCA CCATTGGCAA ATTCGATAGC CAAATCGAGC TCAATGAGCT GTGCCGTCAG 
CACGAGTCCT TAAAAGTTCG CTACGGGTCT ACTCTGTACG AGTCCCGTGC AATTTTCTTT 
GGGCCCGATG AGCAGCCACT GGAAACCATT GGGGAGGTGC TGGGACCACC GGCAGCTGGT 
GGACGACGCC TTCAAGATGA ATTCACAACG CCATCGAACT TCAAAGCGCA GGCCTTTTTC 
TATCGCGAAC AGGATTCGTG TGCCGATCTC GAATCTCGAA TTGGGGACTT TGCTAGCGCG 
TTGTTTTGGG TCCTGGAATC ACGACGGTTA GAGCGATCTC GCGAGAAGGC GGACAAGGTG 
AGCCTGTTGC TGGCACCCTT TGAAAAGCGA GACTTTGTGG GCCTGGACAT GGAGTCGAGG 
AACAATAGGA AAAGATGCGT GGGTCGTGTG ATGAAGAACC TGGCAGATCT ATCGCTGCAA 
GCTGGCCTGG TTGACGACGC CTTGAGTCTG TACCACAATG CCAATGAGAC TTTGAGGTCG 
GTAGGTGATT CCCTTTGGGT TGGGGCAACC GAAGAGGGCT TGTGTGCCGC TTCCGCAATG 
CTGTTGTATC CCCAAATGCG AGAAACCGAA ACGCTGCATA GAAACTCGTC GCTTCAGGAG 
GCGGGCACCT CTCCCCTAAA AAACACCCCT GAAAAGTGGC GTGCTAGCGA CGCCACCAAG 
AAGATTAGCG CCAGTGACGC GACCGCGAAC AATGTGGATT CCAACCAGCC TCAGCAGCGC 
GTAACCTCGA ACTCATCTTC CTGCTCATCC GTGTCTTCCC TGGTGACAAC GGCCACCAAT 
TCCTCCGCCT CGGACACGCC AACTACATCA TCTTCCTCCA CTTCGACGAT ATCGGCAGCA 
CCGATACCAG GACACCAGAG GAACGGCGAT CTGCCGGGCA ACATCCTCAA GGCGGAGGAG 
ATCAGCAACT ACTATCGCAA GGCCATCATT AACTACAGCA AGTACAGGCA TGCAGCCACT 
ATAGAGACGG AGGCGGCACT GAAGGCCTCG CGGATTTGCA TCGAGCAGAA TCGTCCCCTG 
GACGTGGCCA TGTTTTTGCA GAATATACTC TACATAAACC TGAGCATGAG CGAAGCCGAG 
CGGGTAAAGA GATTCGAGGT CATTACGGAT CTTTACCAGC AGATCGGATA CCAAAGGAAG 
GCGGCATTTT TCCAAAGACT CGCTGCACTC AAGCACGTTC AGCAGGGCAG CCAAGCGCCG 
GACTGGAACC AAAGTTACCG CCTCATGTTG GGCAGTTTTA CGGGGTACCG GCTTTGTCTC 
GACCCCTTGG AAGTAATAGA AAATGCCGCC GGCTGGCCGG CGTTGCAAAT CGATCTGGTC 
CAGACCCTTA TAACTGCCGC CAGGCGTTTG GGACACTCCG CTTTGGCCAC GCGGCACATG 
ACCTTTTTGC TGCAGACTCA GTGGGACAAC ATGTCGCCAA CGGAGCAAAG CGAGATGGCT 
GTGCAACTGC AGAATTTAAG CGCTCAGTGC GAGGGCTCTC CTGTGCCCTT GGTGCTGGAA 
AATGGCACTG TGATACCACC CGCCAATCTC ACGGATCTGC CCTACTGTAT CGATCTTCAG 
GTTAAAGATT TGCCCGCTCA TTTGCGACCG CAAAGAATAA AGGTGGCCAA AGCGGACAGT 
GGGCCTTTCC TATTCACCCC CATACACTTT AATTCTGTCG ACAGGAGGGA TAAGAAAAAG 
GACAAAAATA AAATAGCTTT CCAGTGGGTG CAAAATGATC TTAGTGAAGT GACTGTGCGC 
CTGCGGAATC CCCTGCCCTT TGAGCTGCCC GTAACGGACA TGAGATTGCT AACAAACGGC 
GTGGTGTTTG AGTCTTTGCC CCAGACTCTT GTACTACAGC CACATGTGCC CACGTATGTG 
GCGCTACATG GAACGCCAAT TGAGACGGGG CAACTCGATC TGCAGGGCTA CAGCACGCAC 
ACGCTCGGTG TGAAGTCCAA CTGTCGGCTG AAGCACATGC GTGGTCGTAG CTTTCCGCCC 
AACTACGTGG TTGATGTTAT CCCCGCCCTT CCTAGGATAT CTGTGAAAAC TTCGCTACCA 
CAGACGGCGA CGTTTAGCAA CATGAACAGT GCGGATATCG TTGTGACCTC TGCCAGCTTA 
ACGCTGTACA ACGGAGAATC CTCCAGCTGC ACAATAACTA TTACCAATGA GAGCGCCACG 
CTGCCACTGG AGCACCTAGA GTTTAGCATC AACTCAAATG TGGAGCAGGA GCTGCAGCAG 
AAGATCTTCC GAATAGATGA AGAAGCCATC AAGGCCCATT TGCCAGTGCC TCCCCAGGGC 
ACCATTGAAA TCATAGTGGA TGTTTTTGCC GAAGCGGACT TCGTTTGTCC TCAACCACCT 
GCTTCATTGC ATTCTGCCGC TGCTCCTGGG GACTACGGTG CGTCGTCACT AACGCACTAC 
TCGAGCGTGT CCACCTCTGG GCACGCCAGC TTACCCTCGA GAGTCGGTTC ACCGCATCAT 
CGTCGAAATG AGCCACAGAA TTCCAGTTTC CGGTCGACCA TTTCTGGCGG TCCACCATCG 
CTGGCTGCCT TAACCCTGCA CCCTGGCGGC GGTGGAGGAG TAGGACCTTC CAGCCTTGGG 
TCACAATATA ACCAGCACAT AGAGGCGCAA GTTAGATTTA AGTACTCGGG CGGAGATGCT 
TTGACGGCGG GCTACTGTCG CCAATGTGCG GTGTCCTTTA ATCTAGAACT TTTGCCTAGC 
GTACAGATTA CAAGCTGGGA TGTGCTTCCC GCAGAGGTAG CCTCCCAATT TTACCTGGTA 
CTTGACATCT CCAACCTAAC CGCTCAAGAG ATGTCGCTCA ACTATACGGA CACCAAGAAT 
ATACTCATCG AGGCCAAAGA GAGCTGCCGA GTGCCCATAC CCGTGGATCG GTGCTCCTTG 
GAGAAAGTGG TGGCTGCTCG GGCGGCCGAG GTAGCTGAGA ATCTGGAGAG GGAACTCTGC 
TTCCGGACGC AGCTGCTGTC GTTTAACGAT GCTCTGAGTA AGCTCTGCTC AATTCATATA 
GCTGAAAGGG TGAAAATCAA GTGGCTGTTA ACGGGGACGG ACATTCAGGG AATCGCCTCA 
CTGCGAGGAA TAGTTCTCTC CCAGTCTATG GTGGATTTGA CAGCTGTGTC GCCTTTGGAG 
TGGGCTATCA GCTTTCAAGA CACGCTCGTG CAGCCACACA ATGAGATTGT ATGCACGGTG 
GGACAGCGGT CGCTGCTAAG CATCCAGCTG GCCAATCAAT CCCTGCAACC ACTTAGAAAT 
CTGGTGCTGA GCATTAAGTT CTATCAGGAT TACCTGAACG GAATGGAAAA TTACAATTTG 
GAGACACGCG TGGCCATTTC AGGACCAAAT AGAATTGCAA TACCACTACT GGAGAAGCAG 
GAGCAGAAAG AGCATACTTG CTCTGTGATA TTCTTTACCC CCGGACGTTT CAAGGCCAGC 
ATCGAATGCA CCAGCAATCC GCAGAAGCAG TCGGAGCAGC CGTCGTCATT GCTAACCCGC 
TCCTGTCCGG CAGAAGCGGA GAGTGTCGGC CAATCGGTCA TGTTCTCCTC CAGCTACGAT 
GAGCAGCAGG CGCATGTGTG GAAGTTCATT CCGCCCATCG AGGTGACCGT CGTGGAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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brun cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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