Pol32 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pol32
Entrez Gene ID	34892
Full Name	Pol32 polymerase delta subunit
Synonyms	BG:DS07295.3,BcDNA:GH08865,CG3975,Dmel\CG3975,pol32,rd
General protein information	Preferred Names Pol32 polymerase delta subunit Names CG3975 gene product from transcript CG3975-RB CG3975-PB CG3975-PC Pol32-PB Pol32-PC reduced
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35C5-35C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001201887.2	NP_001188816.1	Pol32 polymerase delta subunit, isoform B
NM_135899.3	NP_609743.1	Pol32 polymerase delta subunit, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pol32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pol32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14709	NM_135899.3 Latest version!	Drosophila melanogaster Pol32 polymerase delta subunit (Pol32), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa14709	NM_001201887.2 Latest version!	Drosophila melanogaster Pol32 polymerase delta subunit (Pol32), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pol32 ( NM_001201887.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188816.1 Drosophila melanogaster Pol32 polymerase delta subunit (Pol32), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14709
Clone ID Related Accession (Same CDS sequence)	NM_001201887.2 , NM_135899.3
Accession Version	NM_001201887.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Pol32 polymerase delta subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001201887.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCTTA AAAAGGCTCT CGACGATTGC ATGATCGATT TCGATCGCTG TGTGCTGGTC 
ACCGATCTTC TGGAGGAGTA CAAACTGTCC TACAAGGAGG TGAACGATGT GCTGGAGGCC 
TACATCAAAG AGCAGGAACC TGCCACAAAA TTCGAGAAGA GATTTCTAGT CCATGGCAAG 
CGTAAAACGC AAGGATCCGA TTCCGGCGAA GATCTGTACT CTGTGGTGCT GGAGAGCAGG 
ATGCAGGATT GGCTGGCCAA AGTCCAGGAT GCGGAGTCTC AGCTGTATAG TGTGAAAATA 
GCCGGTGGCA CCAAAGCGCC GGCTGCGATT TTCAAGCCCA TGCAGCATTT GGAGGTGAAG 
TTGGCAAAAG TGGAGCAGCG TCCTGGAGCG GGAAAGATAG TGCCCAGTGC AAATGGGACG 
ACTCCCCACA ATGGAGTCAA ATCTGAACCC ACTAAATCGG AGCCATCAAA GTCTGCGGTT 
AAGTTGGAGC CTTCAAAATC TTCACTTAAA TCAGAGCCAG CAAAGTCGAA GGCGGAAAAG 
CCAGTGGCCA GCAAGTCCTC TCCGGAGGAT AAGAAGACCT CGCCCAAGGA ACAGGCCTCT 
AAAGCCAAGC CTGCAGCAGC CAAAAAGGGT AGCATTAATA GCTTCTTCAC AGCGGCGGCC 
AGCAAGCCAA AGGATGTCAA AGCCACGCCC AGCAAATCGA CGTCCGGCAC CGTGGACAAC 
TTCTTTAAGA AGCAACCGGC AGGCGCCAAG AAGTCGCCAC CGGAATCCGA AGATAAATCG 
AAGAAAGATG CTTCCAATTC AAACAAAAAG GAAGCCTCGA AAAAGAAATC CCCCAGTCCC 
ACCAAAAAGC CAACAACGGC AAACACTTCC ATGCAACTGT TCGACGAGGA GAGTGCCGAA 
TCGTCCGACG AGGAGGAAAA GCTGGACATG CTGCGGCGGA AGGTGATCGA GTCGGACAAC 
GACTCCGATC AGGAAAAGGC CTCGTCCTCC AAGCGGCGAC GAATCAGCGA CTCTGAGGAC 
GAGGAGCAGC CGCCCAAGAA GTCCGCCGAT GAAGAGACCA TCGCATTGGA TGAGAAAATG 
GATACGGAAC CGGCCAATGA AACCTATCTG GATGAAGATG GCTTCGTCAT TACGCAGCGT 
AAACCAACAA AAGCCCAGCC TGCCAACAAA AAGGTTTCCC CAAAGGCTGC TGCTCCCGTC 
AACAAAAAGA AGTCACCGCC TTCAGCCGCG AAAGCTGGAA AGGATGCGCC GAAAACCAAA 
CAAGCTGGCA TTATGAACTT TTTTTCCAAG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188816.1
CDS	42..1337
Translation	MSLKKALDDCMIDFDRCVLVTDLLEEYKLSYKEVNDVLEAYIKEQEPATKFEKRFLVHGKRKTQGSDSGEDLYSVVLESRMQDWLAKVQDAESQLYSVKIAGGTKAPAAIFKPMQHLEVKLAKVEQRPGAGKIVPSANGTTPHNGVKSEPTKSEPSKSAVKLEPSKSSLKSEPAKSKAEKPVASKSSPEDKKTSPKEQASKAKPAAAKKGSINSFFTAAASKPKDVKATPSKSTSGTVDNFFKKQPAGAKKSPPESEDKSKKDASNSNKKEASKKKSPSPTKKPTTANTSMQLFDEESAESSDEEEKLDMLRRKVIESDNDSDQEKASSSKRRRISDSEDEEQPPKKSADEETIALDEKMDTEPANETYLDEDGFVITQRKPTKAQPANKKVSPKAAAPVNKKKSPPSAAKAGKDAPKTKQAGIMNFFSKK

Target ORF information:

RefSeq Version	NM_001201887.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Pol32 polymerase delta subunit (Pol32), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201887.2

ORF Insert Sequence:

ATGTCGCTTA AAAAGGCTCT CGACGATTGC ATGATCGATT TCGATCGCTG TGTGCTGGTC 
ACCGATCTTC TGGAGGAGTA CAAACTGTCC TACAAGGAGG TGAACGATGT GCTGGAGGCC 
TACATCAAAG AGCAGGAACC TGCCACAAAA TTCGAGAAGA GATTTCTAGT CCATGGCAAG 
CGTAAAACGC AAGGATCCGA TTCCGGCGAA GATCTGTACT CTGTGGTGCT GGAGAGCAGG 
ATGCAGGATT GGCTGGCCAA AGTCCAGGAT GCGGAGTCTC AGCTGTATAG TGTGAAAATA 
GCCGGTGGCA CCAAAGCGCC GGCTGCGATT TTCAAGCCCA TGCAGCATTT GGAGGTGAAG 
TTGGCAAAAG TGGAGCAGCG TCCTGGAGCG GGAAAGATAG TGCCCAGTGC AAATGGGACG 
ACTCCCCACA ATGGAGTCAA ATCTGAACCC ACTAAATCGG AGCCATCAAA GTCTGCGGTT 
AAGTTGGAGC CTTCAAAATC TTCACTTAAA TCAGAGCCAG CAAAGTCGAA GGCGGAAAAG 
CCAGTGGCCA GCAAGTCCTC TCCGGAGGAT AAGAAGACCT CGCCCAAGGA ACAGGCCTCT 
AAAGCCAAGC CTGCAGCAGC CAAAAAGGGT AGCATTAATA GCTTCTTCAC AGCGGCGGCC 
AGCAAGCCAA AGGATGTCAA AGCCACGCCC AGCAAATCGA CGTCCGGCAC CGTGGACAAC 
TTCTTTAAGA AGCAACCGGC AGGCGCCAAG AAGTCGCCAC CGGAATCCGA AGATAAATCG 
AAGAAAGATG CTTCCAATTC AAACAAAAAG GAAGCCTCGA AAAAGAAATC CCCCAGTCCC 
ACCAAAAAGC CAACAACGGC AAACACTTCC ATGCAACTGT TCGACGAGGA GAGTGCCGAA 
TCGTCCGACG AGGAGGAAAA GCTGGACATG CTGCGGCGGA AGGTGATCGA GTCGGACAAC 
GACTCCGATC AGGAAAAGGC CTCGTCCTCC AAGCGGCGAC GAATCAGCGA CTCTGAGGAC 
GAGGAGCAGC CGCCCAAGAA GTCCGCCGAT GAAGAGACCA TCGCATTGGA TGAGAAAATG 
GATACGGAAC CGGCCAATGA AACCTATCTG GATGAAGATG GCTTCGTCAT TACGCAGCGT 
AAACCAACAA AAGCCCAGCC TGCCAACAAA AAGGTTTCCC CAAAGGCTGC TGCTCCCGTC 
AACAAAAAGA AGTCACCGCC TTCAGCCGCG AAAGCTGGAA AGGATGCGCC GAAAACCAAA 
CAAGCTGGCA TTATGAACTT TTTTTCCAAG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pol32 ( NM_135899.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609743.1 Drosophila melanogaster Pol32 polymerase delta subunit (Pol32), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa14709
Clone ID Related Accession (Same CDS sequence)	NM_001201887.2 , NM_135899.3
Accession Version	NM_135899.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Pol32 polymerase delta subunit, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135899.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCTTA AAAAGGCTCT CGACGATTGC ATGATCGATT TCGATCGCTG TGTGCTGGTC 
ACCGATCTTC TGGAGGAGTA CAAACTGTCC TACAAGGAGG TGAACGATGT GCTGGAGGCC 
TACATCAAAG AGCAGGAACC TGCCACAAAA TTCGAGAAGA GATTTCTAGT CCATGGCAAG 
CGTAAAACGC AAGGATCCGA TTCCGGCGAA GATCTGTACT CTGTGGTGCT GGAGAGCAGG 
ATGCAGGATT GGCTGGCCAA AGTCCAGGAT GCGGAGTCTC AGCTGTATAG TGTGAAAATA 
GCCGGTGGCA CCAAAGCGCC GGCTGCGATT TTCAAGCCCA TGCAGCATTT GGAGGTGAAG 
TTGGCAAAAG TGGAGCAGCG TCCTGGAGCG GGAAAGATAG TGCCCAGTGC AAATGGGACG 
ACTCCCCACA ATGGAGTCAA ATCTGAACCC ACTAAATCGG AGCCATCAAA GTCTGCGGTT 
AAGTTGGAGC CTTCAAAATC TTCACTTAAA TCAGAGCCAG CAAAGTCGAA GGCGGAAAAG 
CCAGTGGCCA GCAAGTCCTC TCCGGAGGAT AAGAAGACCT CGCCCAAGGA ACAGGCCTCT 
AAAGCCAAGC CTGCAGCAGC CAAAAAGGGT AGCATTAATA GCTTCTTCAC AGCGGCGGCC 
AGCAAGCCAA AGGATGTCAA AGCCACGCCC AGCAAATCGA CGTCCGGCAC CGTGGACAAC 
TTCTTTAAGA AGCAACCGGC AGGCGCCAAG AAGTCGCCAC CGGAATCCGA AGATAAATCG 
AAGAAAGATG CTTCCAATTC AAACAAAAAG GAAGCCTCGA AAAAGAAATC CCCCAGTCCC 
ACCAAAAAGC CAACAACGGC AAACACTTCC ATGCAACTGT TCGACGAGGA GAGTGCCGAA 
TCGTCCGACG AGGAGGAAAA GCTGGACATG CTGCGGCGGA AGGTGATCGA GTCGGACAAC 
GACTCCGATC AGGAAAAGGC CTCGTCCTCC AAGCGGCGAC GAATCAGCGA CTCTGAGGAC 
GAGGAGCAGC CGCCCAAGAA GTCCGCCGAT GAAGAGACCA TCGCATTGGA TGAGAAAATG 
GATACGGAAC CGGCCAATGA AACCTATCTG GATGAAGATG GCTTCGTCAT TACGCAGCGT 
AAACCAACAA AAGCCCAGCC TGCCAACAAA AAGGTTTCCC CAAAGGCTGC TGCTCCCGTC 
AACAAAAAGA AGTCACCGCC TTCAGCCGCG AAAGCTGGAA AGGATGCGCC GAAAACCAAA 
CAAGCTGGCA TTATGAACTT TTTTTCCAAG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609743.1
CDS	42..1337
Translation	MSLKKALDDCMIDFDRCVLVTDLLEEYKLSYKEVNDVLEAYIKEQEPATKFEKRFLVHGKRKTQGSDSGEDLYSVVLESRMQDWLAKVQDAESQLYSVKIAGGTKAPAAIFKPMQHLEVKLAKVEQRPGAGKIVPSANGTTPHNGVKSEPTKSEPSKSAVKLEPSKSSLKSEPAKSKAEKPVASKSSPEDKKTSPKEQASKAKPAAAKKGSINSFFTAAASKPKDVKATPSKSTSGTVDNFFKKQPAGAKKSPPESEDKSKKDASNSNKKEASKKKSPSPTKKPTTANTSMQLFDEESAESSDEEEKLDMLRRKVIESDNDSDQEKASSSKRRRISDSEDEEQPPKKSADEETIALDEKMDTEPANETYLDEDGFVITQRKPTKAQPANKKVSPKAAAPVNKKKSPPSAAKAGKDAPKTKQAGIMNFFSKK

Target ORF information:

RefSeq Version	NM_135899.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Pol32 polymerase delta subunit (Pol32), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135899.3

ORF Insert Sequence:

ATGTCGCTTA AAAAGGCTCT CGACGATTGC ATGATCGATT TCGATCGCTG TGTGCTGGTC 
ACCGATCTTC TGGAGGAGTA CAAACTGTCC TACAAGGAGG TGAACGATGT GCTGGAGGCC 
TACATCAAAG AGCAGGAACC TGCCACAAAA TTCGAGAAGA GATTTCTAGT CCATGGCAAG 
CGTAAAACGC AAGGATCCGA TTCCGGCGAA GATCTGTACT CTGTGGTGCT GGAGAGCAGG 
ATGCAGGATT GGCTGGCCAA AGTCCAGGAT GCGGAGTCTC AGCTGTATAG TGTGAAAATA 
GCCGGTGGCA CCAAAGCGCC GGCTGCGATT TTCAAGCCCA TGCAGCATTT GGAGGTGAAG 
TTGGCAAAAG TGGAGCAGCG TCCTGGAGCG GGAAAGATAG TGCCCAGTGC AAATGGGACG 
ACTCCCCACA ATGGAGTCAA ATCTGAACCC ACTAAATCGG AGCCATCAAA GTCTGCGGTT 
AAGTTGGAGC CTTCAAAATC TTCACTTAAA TCAGAGCCAG CAAAGTCGAA GGCGGAAAAG 
CCAGTGGCCA GCAAGTCCTC TCCGGAGGAT AAGAAGACCT CGCCCAAGGA ACAGGCCTCT 
AAAGCCAAGC CTGCAGCAGC CAAAAAGGGT AGCATTAATA GCTTCTTCAC AGCGGCGGCC 
AGCAAGCCAA AGGATGTCAA AGCCACGCCC AGCAAATCGA CGTCCGGCAC CGTGGACAAC 
TTCTTTAAGA AGCAACCGGC AGGCGCCAAG AAGTCGCCAC CGGAATCCGA AGATAAATCG 
AAGAAAGATG CTTCCAATTC AAACAAAAAG GAAGCCTCGA AAAAGAAATC CCCCAGTCCC 
ACCAAAAAGC CAACAACGGC AAACACTTCC ATGCAACTGT TCGACGAGGA GAGTGCCGAA 
TCGTCCGACG AGGAGGAAAA GCTGGACATG CTGCGGCGGA AGGTGATCGA GTCGGACAAC 
GACTCCGATC AGGAAAAGGC CTCGTCCTCC AAGCGGCGAC GAATCAGCGA CTCTGAGGAC 
GAGGAGCAGC CGCCCAAGAA GTCCGCCGAT GAAGAGACCA TCGCATTGGA TGAGAAAATG 
GATACGGAAC CGGCCAATGA AACCTATCTG GATGAAGATG GCTTCGTCAT TACGCAGCGT 
AAACCAACAA AAGCCCAGCC TGCCAACAAA AAGGTTTCCC CAAAGGCTGC TGCTCCCGTC 
AACAAAAAGA AGTCACCGCC TTCAGCCGCG AAAGCTGGAA AGGATGCGCC GAAAACCAAA 
CAAGCTGGCA TTATGAACTT TTTTTCCAAG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pol32 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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