mTTF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mTTF
Entrez Gene ID	34837
Full Name	mitochondrial transcription termination factor
Synonyms	BG:DS01068.4,CG18124,DS01068.4,DmTTF,Dmel\CG18124
General protein information	Preferred Names mitochondrial transcription termination factor Names CG18124 gene product from transcript CG18124-RA CG18124-PA mTTF-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35A4-35A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_135865.4	NP_609709.1	mitochondrial transcription termination factor

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008874	XP_319620.4
Drosophila melanogaster (fruit fly)	mTTF	NP_609709.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mTTF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mTTF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16776	NM_135865.4 Latest version!	Drosophila melanogaster mitochondrial transcription termination factor (mTTF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mTTF ( NM_135865.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609709.1 Drosophila melanogaster mitochondrial transcription termination factor (mTTF), mRNA.

Price & Availability↑

CloneID	OFa16776
Clone ID Related Accession (Same CDS sequence)	NM_135865.4
Accession Version	NM_135865.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial transcription termination factor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135865.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTAGAA GCCTTCTGCG CAGCTTTGAG ACGGCACTCA AGCTGCATGC CGGCTTAAAT 
ATGCACCCAA TGCACTGCAG CAGGCGCCTA CTCTTCTCGC AGTACGAAAA CCGCGCCAGT 
CCGAGCAGAC TGACCTCCAG TGGCACTTTA GGTAGCAACG AGGCGGAAAA CGATTATGTG 
CCTTATCGCC AGGATCGGGA GACTGGAACC AAGACGCGGG TGCTCCTCGA AGCTCTGCGG 
GAACGTTTTC GCTTCACGGA CGCTGAGCTC CAAAAGATAA TCAGCGACGA GCTGGTGCAC 
CGCTGCTACC GCGGTAGATC ACTGACTCTG GTCATGGACA CACTGCAGCT AGAGGGCGTT 
AGCAGGCGCA GCTTCGTGGA GTATCCTTGG CTACTGTCGC TGGACAATAA ACGCTTGGAG 
CTAAAGATGC AGCTACTCAA GTCAATGGAC TTTAAGGACA TCAATCATTT TGTGCCTTTC 
CTGCGCCTCA CTGTTCCACG TCTTCGAAAA CTGGTGGGTG CTCTGAACTC TGAGAGGGAT 
GCAATGCCAC AGCGAAATCG TGTCTACTAT ATCAGCGAAA AGCTGGATGT CAGCCCCGAT 
ATTGTGAGCA AATATCTATC GAAGAGGCTG TTTATCCTGG AAATGCCGTT CGAGATGTTC 
GAAAAGAACC TGCAGCACAT GATCGACTAC AATGTGTCGC CAATAAACGT ACTCAAGGAT 
CTATGGGCCT TTCGCTATAC TCCAAAGTCT GTTCAGTTGC GTTTGGAGCG CGCCAAGCGG 
GCGAAAAAGG ACAAGATCAT GCCGTGGATG GTGCGCTGCC CGGAGCCAAT ACTGCAGCGC 
TCCCTGAAGC TTTCTCTCGA CGAACTGAAG GTTTTGGGAG AGTTCTCCTC CGTGGTGGAA 
TACTTAGCGC ATCGTCTTGG TTTCAGCACC AGTGAAGCCA AGGCAATCAT GGACAAACAT 
CCGCAGGTGC ACACCGTGCG AGTTACCAAG ATCAAAGAGG TGCTAGACTA CCTACTCGAT 
GAGGCCCAGT TTACCAGATT TGAGGTCGCT CAAAATCCTC GAATTCTATG CCACAGCCTG 
AAAACCACAA AGGAGCGCAT GGAAGAACTG AAATCGCACG GTTGTCGACC CTCCTCTCTT 
GTAATTCTTT GCCGCAGCCG GAGGGAGTAC GATAAGTTTT TGCAGAACTG GATAAGCCAT 
GAAAGAAATC CGCAAAGTGT ATCAGAAGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609709.1
CDS	85..1317
Translation	MIRSLLRSFETALKLHAGLNMHPMHCSRRLLFSQYENRASPSRLTSSGTLGSNEAENDYVPYRQDRETGTKTRVLLEALRERFRFTDAELQKIISDELVHRCYRGRSLTLVMDTLQLEGVSRRSFVEYPWLLSLDNKRLELKMQLLKSMDFKDINHFVPFLRLTVPRLRKLVGALNSERDAMPQRNRVYYISEKLDVSPDIVSKYLSKRLFILEMPFEMFEKNLQHMIDYNVSPINVLKDLWAFRYTPKSVQLRLERAKRAKKDKIMPWMVRCPEPILQRSLKLSLDELKVLGEFSSVVEYLAHRLGFSTSEAKAIMDKHPQVHTVRVTKIKEVLDYLLDEAQFTRFEVAQNPRILCHSLKTTKERMEELKSHGCRPSSLVILCRSRREYDKFLQNWISHERNPQSVSEG

Target ORF information:

RefSeq Version	NM_135865.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial transcription termination factor (mTTF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135865.4

ORF Insert Sequence:

ATGATTAGAA GCCTTCTGCG CAGCTTTGAG ACGGCACTCA AGCTGCATGC CGGCTTAAAT 
ATGCACCCAA TGCACTGCAG CAGGCGCCTA CTCTTCTCGC AGTACGAAAA CCGCGCCAGT 
CCGAGCAGAC TGACCTCCAG TGGCACTTTA GGTAGCAACG AGGCGGAAAA CGATTATGTG 
CCTTATCGCC AGGATCGGGA GACTGGAACC AAGACGCGGG TGCTCCTCGA AGCTCTGCGG 
GAACGTTTTC GCTTCACGGA CGCTGAGCTC CAAAAGATAA TCAGCGACGA GCTGGTGCAC 
CGCTGCTACC GCGGTAGATC ACTGACTCTG GTCATGGACA CACTGCAGCT AGAGGGCGTT 
AGCAGGCGCA GCTTCGTGGA GTATCCTTGG CTACTGTCGC TGGACAATAA ACGCTTGGAG 
CTAAAGATGC AGCTACTCAA GTCAATGGAC TTTAAGGACA TCAATCATTT TGTGCCTTTC 
CTGCGCCTCA CTGTTCCACG TCTTCGAAAA CTGGTGGGTG CTCTGAACTC TGAGAGGGAT 
GCAATGCCAC AGCGAAATCG TGTCTACTAT ATCAGCGAAA AGCTGGATGT CAGCCCCGAT 
ATTGTGAGCA AATATCTATC GAAGAGGCTG TTTATCCTGG AAATGCCGTT CGAGATGTTC 
GAAAAGAACC TGCAGCACAT GATCGACTAC AATGTGTCGC CAATAAACGT ACTCAAGGAT 
CTATGGGCCT TTCGCTATAC TCCAAAGTCT GTTCAGTTGC GTTTGGAGCG CGCCAAGCGG 
GCGAAAAAGG ACAAGATCAT GCCGTGGATG GTGCGCTGCC CGGAGCCAAT ACTGCAGCGC 
TCCCTGAAGC TTTCTCTCGA CGAACTGAAG GTTTTGGGAG AGTTCTCCTC CGTGGTGGAA 
TACTTAGCGC ATCGTCTTGG TTTCAGCACC AGTGAAGCCA AGGCAATCAT GGACAAACAT 
CCGCAGGTGC ACACCGTGCG AGTTACCAAG ATCAAAGAGG TGCTAGACTA CCTACTCGAT 
GAGGCCCAGT TTACCAGATT TGAGGTCGCT CAAAATCCTC GAATTCTATG CCACAGCCTG 
AAAACCACAA AGGAGCGCAT GGAAGAACTG AAATCGCACG GTTGTCGACC CTCCTCTCTT 
GTAATTCTTT GCCGCAGCCG GAGGGAGTAC GATAAGTTTT TGCAGAACTG GATAAGCCAT 
GAAAGAAATC CGCAAAGTGT ATCAGAAGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mTTF cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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