hgo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	hgo
Entrez Gene ID	34552
Full Name	homogentisate 1,2-dioxygenase
Synonyms	CG4779,Dmel\CG4779
General protein information	Preferred Names homogentisate 1,2-dioxygenase Names CG4779 gene product from transcript CG4779-RA CG4779-PA hgo-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 32D5-32D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_078820.3	NP_523544.2	homogentisate 1,2-dioxygenase

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os06g0103300	NP_001056540.1
Drosophila melanogaster (fruit fly)	hgo	NP_523544.2
Homo sapiens (human)	HGD	NP_000178.2
Bos taurus (cattle)	HGD	NP_001138324.1
Danio rerio (zebrafish)	hgd	NP_694498.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009609	XP_318638.4
Caenorhabditis elegans (roundworm)	hgo-1	NP_492433.1
Magnaporthe oryzae (rice blast fungus)	MGG_00431	XP_003718640.1
Canis lupus familiaris (dog)	HGD	XP_535754.3
Mus musculus (house mouse)	Hgd	NP_038575.2
Gallus gallus (chicken)	HGD	XP_416553.1
Xenopus tropicalis (tropical clawed frog)	hgd	XP_004912503.1
Pan troglodytes (chimpanzee)	HGD	XP_001164938.1
Macaca mulatta (Rhesus monkey)	HGD	XP_001110996.1
Rattus norvegicus (Norway rat)	Hgd	NP_001012145.1
Arabidopsis thaliana (thale cress)	HGO	NP_851187.1
Neurospora crassa	NCU05499	XP_960461.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following hgo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hgo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16665	NM_078820.3 Latest version!	Drosophila melanogaster homogentisate 1,2-dioxygenase (hgo), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hgo ( NM_078820.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523544.2 Drosophila melanogaster homogentisate 1,2-dioxygenase (hgo), mRNA.

Price & Availability↑

CloneID	OFa16665
Clone ID Related Accession (Same CDS sequence)	NM_078820.3
Accession Version	NM_078820.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	homogentisate 1,2-dioxygenase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_078820.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGAAT ACAAGTACCT CTCGGGTTTT GGGTCTCACT TCTCATCTGA GGATGAGCGG 
TATCCCAACT CCTTGCCAGT GGGACAGAAC TCACCTCAAG TGTGCCCATA CAAACTCTAT 
GCGGAGCAGC TATCCGGAAG TGCCTTCACT GCGCCCAGGA CGGAGAATAT GCGCACGTGG 
CTCTACAGGA AGTTGCCCTC TGCCGCGCAC CTTCCATTCC AGCCCTTCAA GGGAGCTGAG 
TACTTTAGTC AAAACTGGGA TGAGCAGCCA CCGAATCCAA ACCAGCTTCG TTGGAAACCC 
TTTGACTTGC CTCCAAAGGA TGGAAAAAAT GTGAACTTTG TGGAAGGCTT GCACACGGTT 
TGTGGAGCGG GAGATCCCAG ATCTCGTCAT GGTCTTGCCA TACACATTTA CTCCTGCAAT 
GGCTCGATGG ATAACTCAGC TTTCTATAAC AGCGATGGTG ATTTTTTGAT TGTGCCCCAG 
CAAGGAGTCT TAGATATAAC CACAGAGTTT GGAAGGATGT CTGTAGCCCC AAATGAGATC 
TGCGTTATAC CACAGGGAAT ACGATTTGCT GTAAATGTGG ATTCTCCTTC TAGGGGTTAT 
ATCCTGGAGG TCTATGATGA TCACTTCGTT TTGCCGGACT TGGGTCCCAT TGGTGCTAAT 
GGTTTGGCCA ATCCCAGAGA TTTCGAGACT CCTGTGGCGT GGTTCGATGA TCGGGATGTA 
AAGGACTTCC AGGTAATTTC GAAATTCCAA GGTCGCCTCT TCGTGGCCAA GCAAAATCAC 
ACCGTCTTCG ATGTGGTGGC TTGGCATGGT AACTATGTGC CCTTCAAGTA CGATCTGTCC 
AAGTTCATGG TGATTAACTC GGTTAGCTTT GACCATTGTG ACCCCAGCAT TTTTACCGTG 
CTCACTTGTC CCAGCTTACG AGCTGGAACG GCCATAGCAG ACTTTGTGAT CTTTCCACCC 
CGTTGGTCAG TGCAGGAGCA CACCTTCAGG CCACCCTATT ACCACAGGAA CTGCATGAGC 
GAGTTCATGG GCCTAATTCT GGGCAAGTAT GAGGCCAAGG AGGATGGCTT TGCGGCAGGA 
GGAGCGACCC TCCATTCCAT GATGACGCCA CATGGTCCAG ATGTCAAGTG CTTTGAAGGT 
GCCTCGAATG CCAAACTGGT GCCGGAACGC GTGGCCGAGG GAACTCAGGC CTTCATGTTC 
GAATCCTCTC TAAGTTTGGC CGTTACCAAG TGGGGCGAGG AGACCTGCCA AAAACTGGAT 
GCCGCATATT ACGAATGCTG GCAGGCGCTG AAGAACAACT TTCAAATAAC TAAAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523544.2
CDS	41..1360
Translation	MSEYKYLSGFGSHFSSEDERYPNSLPVGQNSPQVCPYKLYAEQLSGSAFTAPRTENMRTWLYRKLPSAAHLPFQPFKGAEYFSQNWDEQPPNPNQLRWKPFDLPPKDGKNVNFVEGLHTVCGAGDPRSRHGLAIHIYSCNGSMDNSAFYNSDGDFLIVPQQGVLDITTEFGRMSVAPNEICVIPQGIRFAVNVDSPSRGYILEVYDDHFVLPDLGPIGANGLANPRDFETPVAWFDDRDVKDFQVISKFQGRLFVAKQNHTVFDVVAWHGNYVPFKYDLSKFMVINSVSFDHCDPSIFTVLTCPSLRAGTAIADFVIFPPRWSVQEHTFRPPYYHRNCMSEFMGLILGKYEAKEDGFAAGGATLHSMMTPHGPDVKCFEGASNAKLVPERVAEGTQAFMFESSLSLAVTKWGEETCQKLDAAYYECWQALKNNFQITKN

Target ORF information:

RefSeq Version	NM_078820.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster homogentisate 1,2-dioxygenase (hgo), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078820.3

ORF Insert Sequence:

ATGTCGGAAT ACAAGTACCT CTCGGGTTTT GGGTCTCACT TCTCATCTGA GGATGAGCGG 
TATCCCAACT CCTTGCCAGT GGGACAGAAC TCACCTCAAG TGTGCCCATA CAAACTCTAT 
GCGGAGCAGC TATCCGGAAG TGCCTTCACT GCGCCCAGGA CGGAGAATAT GCGCACGTGG 
CTCTACAGGA AGTTGCCCTC TGCCGCGCAC CTTCCATTCC AGCCCTTCAA GGGAGCTGAG 
TACTTTAGTC AAAACTGGGA TGAGCAGCCA CCGAATCCAA ACCAGCTTCG TTGGAAACCC 
TTTGACTTGC CTCCAAAGGA TGGAAAAAAT GTGAACTTTG TGGAAGGCTT GCACACGGTT 
TGTGGAGCGG GAGATCCCAG ATCTCGTCAT GGTCTTGCCA TACACATTTA CTCCTGCAAT 
GGCTCGATGG ATAACTCAGC TTTCTATAAC AGCGATGGTG ATTTTTTGAT TGTGCCCCAG 
CAAGGAGTCT TAGATATAAC CACAGAGTTT GGAAGGATGT CTGTAGCCCC AAATGAGATC 
TGCGTTATAC CACAGGGAAT ACGATTTGCT GTAAATGTGG ATTCTCCTTC TAGGGGTTAT 
ATCCTGGAGG TCTATGATGA TCACTTCGTT TTGCCGGACT TGGGTCCCAT TGGTGCTAAT 
GGTTTGGCCA ATCCCAGAGA TTTCGAGACT CCTGTGGCGT GGTTCGATGA TCGGGATGTA 
AAGGACTTCC AGGTAATTTC GAAATTCCAA GGTCGCCTCT TCGTGGCCAA GCAAAATCAC 
ACCGTCTTCG ATGTGGTGGC TTGGCATGGT AACTATGTGC CCTTCAAGTA CGATCTGTCC 
AAGTTCATGG TGATTAACTC GGTTAGCTTT GACCATTGTG ACCCCAGCAT TTTTACCGTG 
CTCACTTGTC CCAGCTTACG AGCTGGAACG GCCATAGCAG ACTTTGTGAT CTTTCCACCC 
CGTTGGTCAG TGCAGGAGCA CACCTTCAGG CCACCCTATT ACCACAGGAA CTGCATGAGC 
GAGTTCATGG GCCTAATTCT GGGCAAGTAT GAGGCCAAGG AGGATGGCTT TGCGGCAGGA 
GGAGCGACCC TCCATTCCAT GATGACGCCA CATGGTCCAG ATGTCAAGTG CTTTGAAGGT 
GCCTCGAATG CCAAACTGGT GCCGGAACGC GTGGCCGAGG GAACTCAGGC CTTCATGTTC 
GAATCCTCTC TAAGTTTGGC CGTTACCAAG TGGGGCGAGG AGACCTGCCA AAAACTGGAT 
GCCGCATATT ACGAATGCTG GCAGGCGCTG AAGAACAACT TTCAAATAAC TAAAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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hgo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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