AkhR cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	AkhR
Entrez Gene ID	33942
Full Name	Adipokinetic hormone receptor
Synonyms	AKH-R,AKHR,AkhR/GRHR,Akhr,BEST:GH19447,CG11325,DAKHR,DAKHR-1,DGRHR,Dmel\CG11325,GRHR,GRHR/CG11325,GnRHR,akhr
General protein information	Preferred Names Adipokinetic hormone receptor Names CG11325 gene product from transcript CG11325-RC AKH receptor AkhR-PA AkhR-PB AkhR-PC AkhR-PD CG11325-PA CG11325-PB CG11325-PC CG11325-PD Drm-AKH receptor-1 adipokinetic hormone receptor gonadotrophin-releasing hormone receptor gonadotropin-releasing hormone receptor hormone receptor
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 27A1-27A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_058039.4	NP_477387.1	adipokinetic hormone receptor, isoform A
NM_205917.3	NP_995639.1	adipokinetic hormone receptor, isoform C
NM_001273220.1	NP_001260149.1	adipokinetic hormone receptor, isoform D
NM_164705.3	NP_723206.1	adipokinetic hormone receptor, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	GPRGNR1	XP_001687839.2
Danio rerio (zebrafish)	gnrhr1	NP_001138452.1
Gallus gallus (chicken)	CGNRH-R	NP_989984.1
Danio rerio (zebrafish)	gnrhr3	NP_001170921.1
Drosophila melanogaster (fruit fly)	GRHR	NP_723206.1
Xenopus tropicalis (tropical clawed frog)	gnrhr2/nmi	NP_001107548.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AkhR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AkhR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13952	NM_001273220.1 Latest version!	Drosophila melanogaster adipokinetic hormone receptor, transcript variant D (AkhR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa13952	NM_164705.3 Latest version!	Drosophila melanogaster adipokinetic hormone receptor, transcript variant B (AkhR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa13952	NM_058039.4 Latest version!	Drosophila melanogaster adipokinetic hormone receptor, transcript variant A (AkhR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa16320	NM_205917.3 Latest version!	Drosophila melanogaster adipokinetic hormone receptor, transcript variant C (AkhR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AkhR ( NM_058039.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477387.1 Drosophila melanogaster adipokinetic hormone receptor, transcript variant A (AkhR), mRNA.

Price & Availability↑

CloneID	OFa13952
Clone ID Related Accession (Same CDS sequence)	NM_058039.4 , NM_001273220.1 , NM_164705.3
Accession Version	NM_058039.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	adipokinetic hormone receptor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_058039.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAAAAG TAGCTGAGGA GAATGATCAT CGTGATCTAA GTAATTGGTC GAATGTGAAC 
GACACCAATG GCACCATTCA CCTGACCAAG GATATGGTCT TCAATGATGG CCACCGATTG 
TCCATCACCG TGTACAGCAT ACTGTTTGTG ATCTCAACAA TTGGCAACAG CACCGTGCTG 
TATCTGCTGA CCAAGCGGCG ACTGCGCGGT CCCTTGCGTA TTGATATCAT GCTAATGCAT 
CTGGCCATCG CCGATCTAAT GGTGACGCTG CTCCTGATGC CAATGGAGAT CGTGTGGGCC 
TGGACGGTGC AGTGGCTATC CACGGACCTG ATGTGCCGCC TGATGAGCTT TTTCCGCGTC 
TTTGGCCTGT ATCTGTCCAG CTACGTGATG GTCTGCATAT CGCTCGACAG ATACTTTGCC 
ATACTGAAGC CGCTCAAGCG GTCCTACAAC CGGGGACGCA TCATGCTGGC CTGCGCCTGG 
CTGGGATCAG TCGTCTGCAG CATTCCACAG GCCTTTCTCT TCCATCTGGA GGAGCATCCC 
GCCGTCACCG GCTACTTTCA GTGCGTCATC TTCAACTCGT TCAGGAGCGA CTTTGATGAG 
AAGCTGTATC AGGCGGCCTC CATGTGCAGC ATGTACGCCT TTCCGCTGAT CATGTTCATC 
TACTGCTACG GAGCCATTTA CCTGGAGATC TATCGGAAGA GCCAGCGCGT CCTCAAGGAC 
GTGATTGCCG AGCGTTTCCG GCGTTCCAAC GACGACGTGC TCAGCCGGGC CAAGAAGCGC 
ACGCTCAAAA TGACCATCAC GATTGTGATT GTGTTCATCA TCTGCTGGAC TCCGTACTAC 
ACCATCTCCA TGTGGTACTG GCTGGACAAG CACTCCGCGG GCAAGATCAA TCCCCTGCTG 
CGCAAGGCGC TGTTCATCTT CGCCTCGACC AACTCATGTA TGAATCCATT GGTCTACGGA 
CTCTACAACA TTCGCGGGCG AATGAACAAC AACAATCCGT CGGTGAACAA CAGGCACACC 
TCGCTGTCCA ATCGCCTGGA CTCCTCCAAT CAGCTGATGC AAAAGCAGTT GACCAACAAC 
TCGCTGCTGA ATGGAAGAGG CCAGGTGATG GCAGCCGCGG TTTCAGCCAC CACTAAGTTG 
GCCAATGTGG TCAGCCTTAA AGGCACTGCC AATGGCAATG GCTCAGCTGC AGCCGCGGGA 
ACAGTTCCAA TAACTCCTCC GCTGACAGTA ACCATCGCTC CGCTGGCCAC CGACGACGAG 
GCCAACGATG ATTCCTGTCT CAGTGCGGTC ACCATCAGGT GCCAGGATCA ATCCCCGATC 
CGCCAGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477387.1
CDS	238..1569
Translation	MAKVAEENDHRDLSNWSNVNDTNGTIHLTKDMVFNDGHRLSITVYSILFVISTIGNSTVLYLLTKRRLRGPLRIDIMLMHLAIADLMVTLLLMPMEIVWAWTVQWLSTDLMCRLMSFFRVFGLYLSSYVMVCISLDRYFAILKPLKRSYNRGRIMLACAWLGSVVCSIPQAFLFHLEEHPAVTGYFQCVIFNSFRSDFDEKLYQAASMCSMYAFPLIMFIYCYGAIYLEIYRKSQRVLKDVIAERFRRSNDDVLSRAKKRTLKMTITIVIVFIICWTPYYTISMWYWLDKHSAGKINPLLRKALFIFASTNSCMNPLVYGLYNIRGRMNNNNPSVNNRHTSLSNRLDSSNQLMQKQLTNNSLLNGRGQVMAAAVSATTKLANVVSLKGTANGNGSAAAAGTVPITPPLTVTIAPLATDDEANDDSCLSAVTIRCQDQSPIRQK

Target ORF information:

RefSeq Version	NM_058039.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster adipokinetic hormone receptor, transcript variant A (AkhR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058039.4

ORF Insert Sequence:

ATGGCAAAAG TAGCTGAGGA GAATGATCAT CGTGATCTAA GTAATTGGTC GAATGTGAAC 
GACACCAATG GCACCATTCA CCTGACCAAG GATATGGTCT TCAATGATGG CCACCGATTG 
TCCATCACCG TGTACAGCAT ACTGTTTGTG ATCTCAACAA TTGGCAACAG CACCGTGCTG 
TATCTGCTGA CCAAGCGGCG ACTGCGCGGT CCCTTGCGTA TTGATATCAT GCTAATGCAT 
CTGGCCATCG CCGATCTAAT GGTGACGCTG CTCCTGATGC CAATGGAGAT CGTGTGGGCC 
TGGACGGTGC AGTGGCTATC CACGGACCTG ATGTGCCGCC TGATGAGCTT TTTCCGCGTC 
TTTGGCCTGT ATCTGTCCAG CTACGTGATG GTCTGCATAT CGCTCGACAG ATACTTTGCC 
ATACTGAAGC CGCTCAAGCG GTCCTACAAC CGGGGACGCA TCATGCTGGC CTGCGCCTGG 
CTGGGATCAG TCGTCTGCAG CATTCCACAG GCCTTTCTCT TCCATCTGGA GGAGCATCCC 
GCCGTCACCG GCTACTTTCA GTGCGTCATC TTCAACTCGT TCAGGAGCGA CTTTGATGAG 
AAGCTGTATC AGGCGGCCTC CATGTGCAGC ATGTACGCCT TTCCGCTGAT CATGTTCATC 
TACTGCTACG GAGCCATTTA CCTGGAGATC TATCGGAAGA GCCAGCGCGT CCTCAAGGAC 
GTGATTGCCG AGCGTTTCCG GCGTTCCAAC GACGACGTGC TCAGCCGGGC CAAGAAGCGC 
ACGCTCAAAA TGACCATCAC GATTGTGATT GTGTTCATCA TCTGCTGGAC TCCGTACTAC 
ACCATCTCCA TGTGGTACTG GCTGGACAAG CACTCCGCGG GCAAGATCAA TCCCCTGCTG 
CGCAAGGCGC TGTTCATCTT CGCCTCGACC AACTCATGTA TGAATCCATT GGTCTACGGA 
CTCTACAACA TTCGCGGGCG AATGAACAAC AACAATCCGT CGGTGAACAA CAGGCACACC 
TCGCTGTCCA ATCGCCTGGA CTCCTCCAAT CAGCTGATGC AAAAGCAGTT GACCAACAAC 
TCGCTGCTGA ATGGAAGAGG CCAGGTGATG GCAGCCGCGG TTTCAGCCAC CACTAAGTTG 
GCCAATGTGG TCAGCCTTAA AGGCACTGCC AATGGCAATG GCTCAGCTGC AGCCGCGGGA 
ACAGTTCCAA TAACTCCTCC GCTGACAGTA ACCATCGCTC CGCTGGCCAC CGACGACGAG 
GCCAACGATG ATTCCTGTCT CAGTGCGGTC ACCATCAGGT GCCAGGATCA ATCCCCGATC 
CGCCAGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AkhR ( NM_001273220.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260149.1 Drosophila melanogaster adipokinetic hormone receptor, transcript variant D (AkhR), mRNA.

Price & Availability↑

CloneID	OFa13952
Clone ID Related Accession (Same CDS sequence)	NM_058039.4 , NM_001273220.1 , NM_164705.3
Accession Version	NM_001273220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	adipokinetic hormone receptor, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAAAAG TAGCTGAGGA GAATGATCAT CGTGATCTAA GTAATTGGTC GAATGTGAAC 
GACACCAATG GCACCATTCA CCTGACCAAG GATATGGTCT TCAATGATGG CCACCGATTG 
TCCATCACCG TGTACAGCAT ACTGTTTGTG ATCTCAACAA TTGGCAACAG CACCGTGCTG 
TATCTGCTGA CCAAGCGGCG ACTGCGCGGT CCCTTGCGTA TTGATATCAT GCTAATGCAT 
CTGGCCATCG CCGATCTAAT GGTGACGCTG CTCCTGATGC CAATGGAGAT CGTGTGGGCC 
TGGACGGTGC AGTGGCTATC CACGGACCTG ATGTGCCGCC TGATGAGCTT TTTCCGCGTC 
TTTGGCCTGT ATCTGTCCAG CTACGTGATG GTCTGCATAT CGCTCGACAG ATACTTTGCC 
ATACTGAAGC CGCTCAAGCG GTCCTACAAC CGGGGACGCA TCATGCTGGC CTGCGCCTGG 
CTGGGATCAG TCGTCTGCAG CATTCCACAG GCCTTTCTCT TCCATCTGGA GGAGCATCCC 
GCCGTCACCG GCTACTTTCA GTGCGTCATC TTCAACTCGT TCAGGAGCGA CTTTGATGAG 
AAGCTGTATC AGGCGGCCTC CATGTGCAGC ATGTACGCCT TTCCGCTGAT CATGTTCATC 
TACTGCTACG GAGCCATTTA CCTGGAGATC TATCGGAAGA GCCAGCGCGT CCTCAAGGAC 
GTGATTGCCG AGCGTTTCCG GCGTTCCAAC GACGACGTGC TCAGCCGGGC CAAGAAGCGC 
ACGCTCAAAA TGACCATCAC GATTGTGATT GTGTTCATCA TCTGCTGGAC TCCGTACTAC 
ACCATCTCCA TGTGGTACTG GCTGGACAAG CACTCCGCGG GCAAGATCAA TCCCCTGCTG 
CGCAAGGCGC TGTTCATCTT CGCCTCGACC AACTCATGTA TGAATCCATT GGTCTACGGA 
CTCTACAACA TTCGCGGGCG AATGAACAAC AACAATCCGT CGGTGAACAA CAGGCACACC 
TCGCTGTCCA ATCGCCTGGA CTCCTCCAAT CAGCTGATGC AAAAGCAGTT GACCAACAAC 
TCGCTGCTGA ATGGAAGAGG CCAGGTGATG GCAGCCGCGG TTTCAGCCAC CACTAAGTTG 
GCCAATGTGG TCAGCCTTAA AGGCACTGCC AATGGCAATG GCTCAGCTGC AGCCGCGGGA 
ACAGTTCCAA TAACTCCTCC GCTGACAGTA ACCATCGCTC CGCTGGCCAC CGACGACGAG 
GCCAACGATG ATTCCTGTCT CAGTGCGGTC ACCATCAGGT GCCAGGATCA ATCCCCGATC 
CGCCAGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260149.1
CDS	794..2125
Translation	MAKVAEENDHRDLSNWSNVNDTNGTIHLTKDMVFNDGHRLSITVYSILFVISTIGNSTVLYLLTKRRLRGPLRIDIMLMHLAIADLMVTLLLMPMEIVWAWTVQWLSTDLMCRLMSFFRVFGLYLSSYVMVCISLDRYFAILKPLKRSYNRGRIMLACAWLGSVVCSIPQAFLFHLEEHPAVTGYFQCVIFNSFRSDFDEKLYQAASMCSMYAFPLIMFIYCYGAIYLEIYRKSQRVLKDVIAERFRRSNDDVLSRAKKRTLKMTITIVIVFIICWTPYYTISMWYWLDKHSAGKINPLLRKALFIFASTNSCMNPLVYGLYNIRGRMNNNNPSVNNRHTSLSNRLDSSNQLMQKQLTNNSLLNGRGQVMAAAVSATTKLANVVSLKGTANGNGSAAAAGTVPITPPLTVTIAPLATDDEANDDSCLSAVTIRCQDQSPIRQK

Target ORF information:

RefSeq Version	NM_001273220.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster adipokinetic hormone receptor, transcript variant D (AkhR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273220.1

ORF Insert Sequence:

ATGGCAAAAG TAGCTGAGGA GAATGATCAT CGTGATCTAA GTAATTGGTC GAATGTGAAC 
GACACCAATG GCACCATTCA CCTGACCAAG GATATGGTCT TCAATGATGG CCACCGATTG 
TCCATCACCG TGTACAGCAT ACTGTTTGTG ATCTCAACAA TTGGCAACAG CACCGTGCTG 
TATCTGCTGA CCAAGCGGCG ACTGCGCGGT CCCTTGCGTA TTGATATCAT GCTAATGCAT 
CTGGCCATCG CCGATCTAAT GGTGACGCTG CTCCTGATGC CAATGGAGAT CGTGTGGGCC 
TGGACGGTGC AGTGGCTATC CACGGACCTG ATGTGCCGCC TGATGAGCTT TTTCCGCGTC 
TTTGGCCTGT ATCTGTCCAG CTACGTGATG GTCTGCATAT CGCTCGACAG ATACTTTGCC 
ATACTGAAGC CGCTCAAGCG GTCCTACAAC CGGGGACGCA TCATGCTGGC CTGCGCCTGG 
CTGGGATCAG TCGTCTGCAG CATTCCACAG GCCTTTCTCT TCCATCTGGA GGAGCATCCC 
GCCGTCACCG GCTACTTTCA GTGCGTCATC TTCAACTCGT TCAGGAGCGA CTTTGATGAG 
AAGCTGTATC AGGCGGCCTC CATGTGCAGC ATGTACGCCT TTCCGCTGAT CATGTTCATC 
TACTGCTACG GAGCCATTTA CCTGGAGATC TATCGGAAGA GCCAGCGCGT CCTCAAGGAC 
GTGATTGCCG AGCGTTTCCG GCGTTCCAAC GACGACGTGC TCAGCCGGGC CAAGAAGCGC 
ACGCTCAAAA TGACCATCAC GATTGTGATT GTGTTCATCA TCTGCTGGAC TCCGTACTAC 
ACCATCTCCA TGTGGTACTG GCTGGACAAG CACTCCGCGG GCAAGATCAA TCCCCTGCTG 
CGCAAGGCGC TGTTCATCTT CGCCTCGACC AACTCATGTA TGAATCCATT GGTCTACGGA 
CTCTACAACA TTCGCGGGCG AATGAACAAC AACAATCCGT CGGTGAACAA CAGGCACACC 
TCGCTGTCCA ATCGCCTGGA CTCCTCCAAT CAGCTGATGC AAAAGCAGTT GACCAACAAC 
TCGCTGCTGA ATGGAAGAGG CCAGGTGATG GCAGCCGCGG TTTCAGCCAC CACTAAGTTG 
GCCAATGTGG TCAGCCTTAA AGGCACTGCC AATGGCAATG GCTCAGCTGC AGCCGCGGGA 
ACAGTTCCAA TAACTCCTCC GCTGACAGTA ACCATCGCTC CGCTGGCCAC CGACGACGAG 
GCCAACGATG ATTCCTGTCT CAGTGCGGTC ACCATCAGGT GCCAGGATCA ATCCCCGATC 
CGCCAGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AkhR ( NM_164705.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723206.1 Drosophila melanogaster adipokinetic hormone receptor, transcript variant B (AkhR), mRNA.

Price & Availability↑

CloneID	OFa13952
Clone ID Related Accession (Same CDS sequence)	NM_058039.4 , NM_001273220.1 , NM_164705.3
Accession Version	NM_164705.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	adipokinetic hormone receptor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164705.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAAAAG TAGCTGAGGA GAATGATCAT CGTGATCTAA GTAATTGGTC GAATGTGAAC 
GACACCAATG GCACCATTCA CCTGACCAAG GATATGGTCT TCAATGATGG CCACCGATTG 
TCCATCACCG TGTACAGCAT ACTGTTTGTG ATCTCAACAA TTGGCAACAG CACCGTGCTG 
TATCTGCTGA CCAAGCGGCG ACTGCGCGGT CCCTTGCGTA TTGATATCAT GCTAATGCAT 
CTGGCCATCG CCGATCTAAT GGTGACGCTG CTCCTGATGC CAATGGAGAT CGTGTGGGCC 
TGGACGGTGC AGTGGCTATC CACGGACCTG ATGTGCCGCC TGATGAGCTT TTTCCGCGTC 
TTTGGCCTGT ATCTGTCCAG CTACGTGATG GTCTGCATAT CGCTCGACAG ATACTTTGCC 
ATACTGAAGC CGCTCAAGCG GTCCTACAAC CGGGGACGCA TCATGCTGGC CTGCGCCTGG 
CTGGGATCAG TCGTCTGCAG CATTCCACAG GCCTTTCTCT TCCATCTGGA GGAGCATCCC 
GCCGTCACCG GCTACTTTCA GTGCGTCATC TTCAACTCGT TCAGGAGCGA CTTTGATGAG 
AAGCTGTATC AGGCGGCCTC CATGTGCAGC ATGTACGCCT TTCCGCTGAT CATGTTCATC 
TACTGCTACG GAGCCATTTA CCTGGAGATC TATCGGAAGA GCCAGCGCGT CCTCAAGGAC 
GTGATTGCCG AGCGTTTCCG GCGTTCCAAC GACGACGTGC TCAGCCGGGC CAAGAAGCGC 
ACGCTCAAAA TGACCATCAC GATTGTGATT GTGTTCATCA TCTGCTGGAC TCCGTACTAC 
ACCATCTCCA TGTGGTACTG GCTGGACAAG CACTCCGCGG GCAAGATCAA TCCCCTGCTG 
CGCAAGGCGC TGTTCATCTT CGCCTCGACC AACTCATGTA TGAATCCATT GGTCTACGGA 
CTCTACAACA TTCGCGGGCG AATGAACAAC AACAATCCGT CGGTGAACAA CAGGCACACC 
TCGCTGTCCA ATCGCCTGGA CTCCTCCAAT CAGCTGATGC AAAAGCAGTT GACCAACAAC 
TCGCTGCTGA ATGGAAGAGG CCAGGTGATG GCAGCCGCGG TTTCAGCCAC CACTAAGTTG 
GCCAATGTGG TCAGCCTTAA AGGCACTGCC AATGGCAATG GCTCAGCTGC AGCCGCGGGA 
ACAGTTCCAA TAACTCCTCC GCTGACAGTA ACCATCGCTC CGCTGGCCAC CGACGACGAG 
GCCAACGATG ATTCCTGTCT CAGTGCGGTC ACCATCAGGT GCCAGGATCA ATCCCCGATC 
CGCCAGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723206.1
CDS	393..1724
Translation	MAKVAEENDHRDLSNWSNVNDTNGTIHLTKDMVFNDGHRLSITVYSILFVISTIGNSTVLYLLTKRRLRGPLRIDIMLMHLAIADLMVTLLLMPMEIVWAWTVQWLSTDLMCRLMSFFRVFGLYLSSYVMVCISLDRYFAILKPLKRSYNRGRIMLACAWLGSVVCSIPQAFLFHLEEHPAVTGYFQCVIFNSFRSDFDEKLYQAASMCSMYAFPLIMFIYCYGAIYLEIYRKSQRVLKDVIAERFRRSNDDVLSRAKKRTLKMTITIVIVFIICWTPYYTISMWYWLDKHSAGKINPLLRKALFIFASTNSCMNPLVYGLYNIRGRMNNNNPSVNNRHTSLSNRLDSSNQLMQKQLTNNSLLNGRGQVMAAAVSATTKLANVVSLKGTANGNGSAAAAGTVPITPPLTVTIAPLATDDEANDDSCLSAVTIRCQDQSPIRQK

Target ORF information:

RefSeq Version	NM_164705.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster adipokinetic hormone receptor, transcript variant B (AkhR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164705.3

ORF Insert Sequence:

ATGGCAAAAG TAGCTGAGGA GAATGATCAT CGTGATCTAA GTAATTGGTC GAATGTGAAC 
GACACCAATG GCACCATTCA CCTGACCAAG GATATGGTCT TCAATGATGG CCACCGATTG 
TCCATCACCG TGTACAGCAT ACTGTTTGTG ATCTCAACAA TTGGCAACAG CACCGTGCTG 
TATCTGCTGA CCAAGCGGCG ACTGCGCGGT CCCTTGCGTA TTGATATCAT GCTAATGCAT 
CTGGCCATCG CCGATCTAAT GGTGACGCTG CTCCTGATGC CAATGGAGAT CGTGTGGGCC 
TGGACGGTGC AGTGGCTATC CACGGACCTG ATGTGCCGCC TGATGAGCTT TTTCCGCGTC 
TTTGGCCTGT ATCTGTCCAG CTACGTGATG GTCTGCATAT CGCTCGACAG ATACTTTGCC 
ATACTGAAGC CGCTCAAGCG GTCCTACAAC CGGGGACGCA TCATGCTGGC CTGCGCCTGG 
CTGGGATCAG TCGTCTGCAG CATTCCACAG GCCTTTCTCT TCCATCTGGA GGAGCATCCC 
GCCGTCACCG GCTACTTTCA GTGCGTCATC TTCAACTCGT TCAGGAGCGA CTTTGATGAG 
AAGCTGTATC AGGCGGCCTC CATGTGCAGC ATGTACGCCT TTCCGCTGAT CATGTTCATC 
TACTGCTACG GAGCCATTTA CCTGGAGATC TATCGGAAGA GCCAGCGCGT CCTCAAGGAC 
GTGATTGCCG AGCGTTTCCG GCGTTCCAAC GACGACGTGC TCAGCCGGGC CAAGAAGCGC 
ACGCTCAAAA TGACCATCAC GATTGTGATT GTGTTCATCA TCTGCTGGAC TCCGTACTAC 
ACCATCTCCA TGTGGTACTG GCTGGACAAG CACTCCGCGG GCAAGATCAA TCCCCTGCTG 
CGCAAGGCGC TGTTCATCTT CGCCTCGACC AACTCATGTA TGAATCCATT GGTCTACGGA 
CTCTACAACA TTCGCGGGCG AATGAACAAC AACAATCCGT CGGTGAACAA CAGGCACACC 
TCGCTGTCCA ATCGCCTGGA CTCCTCCAAT CAGCTGATGC AAAAGCAGTT GACCAACAAC 
TCGCTGCTGA ATGGAAGAGG CCAGGTGATG GCAGCCGCGG TTTCAGCCAC CACTAAGTTG 
GCCAATGTGG TCAGCCTTAA AGGCACTGCC AATGGCAATG GCTCAGCTGC AGCCGCGGGA 
ACAGTTCCAA TAACTCCTCC GCTGACAGTA ACCATCGCTC CGCTGGCCAC CGACGACGAG 
GCCAACGATG ATTCCTGTCT CAGTGCGGTC ACCATCAGGT GCCAGGATCA ATCCCCGATC 
CGCCAGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AkhR ( NM_205917.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995639.1 Drosophila melanogaster adipokinetic hormone receptor, transcript variant C (AkhR), mRNA.

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CloneID	OFa16320
Clone ID Related Accession (Same CDS sequence)	NM_205917.3
Accession Version	NM_205917.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	adipokinetic hormone receptor, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_205917.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAAAAG TAGCTGAGGA GAATGATCAT CGTGATCTAA GTAATTGGTC GAATGTGAAC 
GACACCAATG GCACCATTCA CCTGACCAAG GATATGGTCT TCAATGATGG CCACCGATTG 
TCCATCACCG TGTACAGCAT ACTGTTTGTG ATCTCAACAA TTGGCAACAG CACCGTGCTG 
TATCTGCTGA CCAAGCGGCG ACTGCGCGGT CCCTTGCGTA TTGATATCAT GCTAATGCAT 
CTGGCCATCG CCGATCTAAT GGTGACGCTG CTCCTGATGC CAATGGAGAT CGTGTGGGCC 
TGGACGGTGC AGTGGCTATC CACGGACCTG ATGTGCCGCC TGATGAGCTT TTTCCGCGTC 
TTTGGCCTGT ATCTGTCCAG CTACGTGATG GTCTGCATAT CGCTCGACAG ATACTTTGCC 
ATACTGAAGC CGCTCAAGCG GTCCTACAAC CGGGGACGCA TCATGCTGGC CTGCGCCTGG 
CTGGGATCAG TCGTCTGCAG CATTCCACAG GCCTTTCTCT TCCATCTGGA GGAGCATCCC 
GCCGTCACCG GCTACTTTCA GTGCGTCATC TTCAACTCGT TCAGGAGCGA CTTTGATGAG 
AAGCTGTATC AGGCGGCCTC CATGTGCAGC ATGTACGCCT TTCCGCTGAT CATGTTCATC 
TACTGCTACG GAGCCATTTA CCTGGAGATC TATCGGAAGA GCCAGCGCGT CCTCAAGGAC 
GTGATTGCCG AGCGTTTCCG GCGTTCCAAC GACGACGTGC TCAGCCGGGC CAAGAAGCGC 
ACGCTCAAAA TGACCATCAC GATTGTGATT GTGTTCATCA TCTGCTGGAC TCCGTACTAC 
ACCATCTCCA TGTGGTACTG GCTGGACAAG CACTCCGCGG GCAAGATCAA TCCCCTGCTG 
CGCAAGGCGC TGTTCATCTT CGCCTCGACC AACTCATGTA TGAATCCATT GGTCTACGGA 
CTCTACAACA TTCGCGGGCG AATGAACAAC AACAATCCGT CGGTGAACAA CAGGCACACC 
TCGCTGTCCA ATCGCCTGGA CTCCTCCAAT CAGCTGATGC AAAAGCAGTT GACCAACAAC 
TCGCTGCTGA ATGGAAGAGG CCAGGTGATG GCAGCCGCGG TTTCAGCCAC CACTAAGTTG 
GCCAATGTGG TCAGCCTTAA AGGCACTGCC AATGGCAATG GCTCAGCTGC AGCCGCGGGA 
ACAGTTCCAA TAACTCCTCC GCTGACAGTA ACCATCGCTC CGCTGGCCAC CGACGACGAG 
GCCAACGATG ATTCCTGTCT CAGTGCGGTC ACCATCAGGT GCCAGGATCA ATCCCCGATC 
CGCCAGAAAT GTGGCGACTC CATCGAATTG ACCAGTGTGG TCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995639.1
CDS	238..1605
Translation	MAKVAEENDHRDLSNWSNVNDTNGTIHLTKDMVFNDGHRLSITVYSILFVISTIGNSTVLYLLTKRRLRGPLRIDIMLMHLAIADLMVTLLLMPMEIVWAWTVQWLSTDLMCRLMSFFRVFGLYLSSYVMVCISLDRYFAILKPLKRSYNRGRIMLACAWLGSVVCSIPQAFLFHLEEHPAVTGYFQCVIFNSFRSDFDEKLYQAASMCSMYAFPLIMFIYCYGAIYLEIYRKSQRVLKDVIAERFRRSNDDVLSRAKKRTLKMTITIVIVFIICWTPYYTISMWYWLDKHSAGKINPLLRKALFIFASTNSCMNPLVYGLYNIRGRMNNNNPSVNNRHTSLSNRLDSSNQLMQKQLTNNSLLNGRGQVMAAAVSATTKLANVVSLKGTANGNGSAAAAGTVPITPPLTVTIAPLATDDEANDDSCLSAVTIRCQDQSPIRQKCGDSIELTSVVK

Target ORF information:

RefSeq Version	NM_205917.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster adipokinetic hormone receptor, transcript variant C (AkhR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205917.3

ORF Insert Sequence:

ATGGCAAAAG TAGCTGAGGA GAATGATCAT CGTGATCTAA GTAATTGGTC GAATGTGAAC 
GACACCAATG GCACCATTCA CCTGACCAAG GATATGGTCT TCAATGATGG CCACCGATTG 
TCCATCACCG TGTACAGCAT ACTGTTTGTG ATCTCAACAA TTGGCAACAG CACCGTGCTG 
TATCTGCTGA CCAAGCGGCG ACTGCGCGGT CCCTTGCGTA TTGATATCAT GCTAATGCAT 
CTGGCCATCG CCGATCTAAT GGTGACGCTG CTCCTGATGC CAATGGAGAT CGTGTGGGCC 
TGGACGGTGC AGTGGCTATC CACGGACCTG ATGTGCCGCC TGATGAGCTT TTTCCGCGTC 
TTTGGCCTGT ATCTGTCCAG CTACGTGATG GTCTGCATAT CGCTCGACAG ATACTTTGCC 
ATACTGAAGC CGCTCAAGCG GTCCTACAAC CGGGGACGCA TCATGCTGGC CTGCGCCTGG 
CTGGGATCAG TCGTCTGCAG CATTCCACAG GCCTTTCTCT TCCATCTGGA GGAGCATCCC 
GCCGTCACCG GCTACTTTCA GTGCGTCATC TTCAACTCGT TCAGGAGCGA CTTTGATGAG 
AAGCTGTATC AGGCGGCCTC CATGTGCAGC ATGTACGCCT TTCCGCTGAT CATGTTCATC 
TACTGCTACG GAGCCATTTA CCTGGAGATC TATCGGAAGA GCCAGCGCGT CCTCAAGGAC 
GTGATTGCCG AGCGTTTCCG GCGTTCCAAC GACGACGTGC TCAGCCGGGC CAAGAAGCGC 
ACGCTCAAAA TGACCATCAC GATTGTGATT GTGTTCATCA TCTGCTGGAC TCCGTACTAC 
ACCATCTCCA TGTGGTACTG GCTGGACAAG CACTCCGCGG GCAAGATCAA TCCCCTGCTG 
CGCAAGGCGC TGTTCATCTT CGCCTCGACC AACTCATGTA TGAATCCATT GGTCTACGGA 
CTCTACAACA TTCGCGGGCG AATGAACAAC AACAATCCGT CGGTGAACAA CAGGCACACC 
TCGCTGTCCA ATCGCCTGGA CTCCTCCAAT CAGCTGATGC AAAAGCAGTT GACCAACAAC 
TCGCTGCTGA ATGGAAGAGG CCAGGTGATG GCAGCCGCGG TTTCAGCCAC CACTAAGTTG 
GCCAATGTGG TCAGCCTTAA AGGCACTGCC AATGGCAATG GCTCAGCTGC AGCCGCGGGA 
ACAGTTCCAA TAACTCCTCC GCTGACAGTA ACCATCGCTC CGCTGGCCAC CGACGACGAG 
GCCAACGATG ATTCCTGTCT CAGTGCGGTC ACCATCAGGT GCCAGGATCA ATCCCCGATC 
CGCCAGAAAT GTGGCGACTC CATCGAATTG ACCAGTGTGG TCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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AkhR cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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