KFase cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	KFase
Entrez Gene ID	33907
Full Name	Kynurenine formamidase
Synonyms	CG9542,Dmel\CG9542
General protein information	Preferred Names Kynurenine formamidase Names CG9542 gene product from transcript CG9542-RB CG9542-PA CG9542-PB KFase-PA KFase-PB kynurenine formamidase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 26D9-26D9

mRNA and Protein(s)

mRNA	Protein	Name
NM_135192.2	NP_609036.1	kynurenine formamidase, isoform A
NM_001298747.1	NP_001285676.1	kynurenine formamidase, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009433	XP_307845.4
Rattus norvegicus (Norway rat)	Afmid	NP_001104836.1
Pan troglodytes (chimpanzee)	AFMID	XP_001156359.2
Macaca mulatta (Rhesus monkey)	LOC715153	XP_001105238.2
Caenorhabditis elegans (roundworm)	D2024.2	NP_501149.1
Caenorhabditis elegans (roundworm)	Y45F10A.3	NP_502605.2
Danio rerio (zebrafish)	LOC100537894	XP_003199584.2
Homo sapiens (human)	AFMID	NP_001010982.2
Bos taurus (cattle)	AFMID	NP_001179431.1
Mus musculus (house mouse)	Afmid	NP_082103.1
Xenopus tropicalis (tropical clawed frog)	afmid	NP_001106623.1
Canis lupus familiaris (dog)	AFMID	XP_851282.2
Danio rerio (zebrafish)	afmid	NP_001017677.1
Gallus gallus (chicken)	AFMID	XP_004946352.1
Drosophila melanogaster (fruit fly)	KFase	NP_609036.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KFase gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KFase cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13919	NM_135192.2 Latest version!	Drosophila melanogaster kynurenine formamidase, transcript variant A (KFase), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OFa13919	NM_001298747.1 Latest version!	Drosophila melanogaster kynurenine formamidase, transcript variant B (KFase), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KFase ( NM_135192.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609036.1 Drosophila melanogaster kynurenine formamidase, transcript variant A (KFase), mRNA.

Price & Availability↑

CloneID	OFa13919
Clone ID Related Accession (Same CDS sequence)	NM_135192.2 , NM_001298747.1
Accession Version	NM_135192.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	kynurenine formamidase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Nov 5, 2002 this sequence version replaced NM_135192.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACAATC CGAGGTGCAA AGACCTTGAC CGGGACTATT TTCCCAGCTA TCATACGACC 
AGGTTTCAGG ATCAGCCGGA ACCGAATTTG GCCGTGCTAG AGCACTTTGT TCGGGTGACC 
AAGCAGCATG GCAGGGAACT GACTGAGAAG CAGGGCATCA CTGTGGACCA CTTGCGCTAC 
GGAGAAGGTC GTCAGTTGGT GGATGTGTTC TACAGCGAAA AGACCACCAA CCAGGCGCCG 
CTTTTCGTCT TCGTGCACGG CGGCTACTGG CAGGAGATGG ACATGAGCAT GTCCTGCTCG 
ATTGTGGGTC CCCTGGTGCG GCGTGGATAC CGAGTGGCCG TCATGGACTA CAACCTGTGC 
CCGCAGGTCA CCCTCGAGCA GCTGATGACC CAGTTCACCC ATTTCCTCAA CTGGATCTTT 
GACTACACCG AAATGACCAA AGTGTCGTCG CTCACCTTCG CCGGACACTC GGCTGGTGCC 
CACTTGCTGG CCCAGATACT CATGCGCCCA AATGTGATTA CAGCCCAGCG GAGCAAGATG 
GTGTGGGCTT TGATCTTCCT CTGCGGCGTT TACGATCTGA GGGAGCTCTC TAACTTGGAA 
TCGGTTAATC CCAAAAATAT TCTGGGCCTC AACGAGCGGA ATATCGAGTC CGTGTCGCCC 
ATGCTGTGGG AATACACCGA TGTTACCGTT TGGAACTCCA CCAAGATCTA CGTGGTGGCC 
GCCGAGCATG ACAGCACCAC CTTCATCGAG CAAAGTCGCC ACTACGCTGA TGTGCTGAGA 
AAGAAGGGCT ACAAGGCGAG CTTTACGCTC TTCAAAGGGT ACGACCACTT CGACATCATC 
GAGGAGACGG CCATCGACGA TTCGGATGTA TCCCGCTTTC TGCGCAACAT TGAAATTGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609036.1
CDS	65..967
Translation	MYNPRCKDLDRDYFPSYHTTRFQDQPEPNLAVLEHFVRVTKQHGRELTEKQGITVDHLRYGEGRQLVDVFYSEKTTNQAPLFVFVHGGYWQEMDMSMSCSIVGPLVRRGYRVAVMDYNLCPQVTLEQLMTQFTHFLNWIFDYTEMTKVSSLTFAGHSAGAHLLAQILMRPNVITAQRSKMVWALIFLCGVYDLRELSNLESVNPKNILGLNERNIESVSPMLWEYTDVTVWNSTKIYVVAAEHDSTTFIEQSRHYADVLRKKGYKASFTLFKGYDHFDIIEETAIDDSDVSRFLRNIEIE

Target ORF information:

RefSeq Version	NM_135192.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kynurenine formamidase, transcript variant A (KFase), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135192.2

ORF Insert Sequence:

ATGTACAATC CGAGGTGCAA AGACCTTGAC CGGGACTATT TTCCCAGCTA TCATACGACC 
AGGTTTCAGG ATCAGCCGGA ACCGAATTTG GCCGTGCTAG AGCACTTTGT TCGGGTGACC 
AAGCAGCATG GCAGGGAACT GACTGAGAAG CAGGGCATCA CTGTGGACCA CTTGCGCTAC 
GGAGAAGGTC GTCAGTTGGT GGATGTGTTC TACAGCGAAA AGACCACCAA CCAGGCGCCG 
CTTTTCGTCT TCGTGCACGG CGGCTACTGG CAGGAGATGG ACATGAGCAT GTCCTGCTCG 
ATTGTGGGTC CCCTGGTGCG GCGTGGATAC CGAGTGGCCG TCATGGACTA CAACCTGTGC 
CCGCAGGTCA CCCTCGAGCA GCTGATGACC CAGTTCACCC ATTTCCTCAA CTGGATCTTT 
GACTACACCG AAATGACCAA AGTGTCGTCG CTCACCTTCG CCGGACACTC GGCTGGTGCC 
CACTTGCTGG CCCAGATACT CATGCGCCCA AATGTGATTA CAGCCCAGCG GAGCAAGATG 
GTGTGGGCTT TGATCTTCCT CTGCGGCGTT TACGATCTGA GGGAGCTCTC TAACTTGGAA 
TCGGTTAATC CCAAAAATAT TCTGGGCCTC AACGAGCGGA ATATCGAGTC CGTGTCGCCC 
ATGCTGTGGG AATACACCGA TGTTACCGTT TGGAACTCCA CCAAGATCTA CGTGGTGGCC 
GCCGAGCATG ACAGCACCAC CTTCATCGAG CAAAGTCGCC ACTACGCTGA TGTGCTGAGA 
AAGAAGGGCT ACAAGGCGAG CTTTACGCTC TTCAAAGGGT ACGACCACTT CGACATCATC 
GAGGAGACGG CCATCGACGA TTCGGATGTA TCCCGCTTTC TGCGCAACAT TGAAATTGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KFase ( NM_001298747.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285676.1 Drosophila melanogaster kynurenine formamidase, transcript variant B (KFase), mRNA.

Price & Availability↑

CloneID	OFa13919
Clone ID Related Accession (Same CDS sequence)	NM_135192.2 , NM_001298747.1
Accession Version	NM_001298747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	kynurenine formamidase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACAATC CGAGGTGCAA AGACCTTGAC CGGGACTATT TTCCCAGCTA TCATACGACC 
AGGTTTCAGG ATCAGCCGGA ACCGAATTTG GCCGTGCTAG AGCACTTTGT TCGGGTGACC 
AAGCAGCATG GCAGGGAACT GACTGAGAAG CAGGGCATCA CTGTGGACCA CTTGCGCTAC 
GGAGAAGGTC GTCAGTTGGT GGATGTGTTC TACAGCGAAA AGACCACCAA CCAGGCGCCG 
CTTTTCGTCT TCGTGCACGG CGGCTACTGG CAGGAGATGG ACATGAGCAT GTCCTGCTCG 
ATTGTGGGTC CCCTGGTGCG GCGTGGATAC CGAGTGGCCG TCATGGACTA CAACCTGTGC 
CCGCAGGTCA CCCTCGAGCA GCTGATGACC CAGTTCACCC ATTTCCTCAA CTGGATCTTT 
GACTACACCG AAATGACCAA AGTGTCGTCG CTCACCTTCG CCGGACACTC GGCTGGTGCC 
CACTTGCTGG CCCAGATACT CATGCGCCCA AATGTGATTA CAGCCCAGCG GAGCAAGATG 
GTGTGGGCTT TGATCTTCCT CTGCGGCGTT TACGATCTGA GGGAGCTCTC TAACTTGGAA 
TCGGTTAATC CCAAAAATAT TCTGGGCCTC AACGAGCGGA ATATCGAGTC CGTGTCGCCC 
ATGCTGTGGG AATACACCGA TGTTACCGTT TGGAACTCCA CCAAGATCTA CGTGGTGGCC 
GCCGAGCATG ACAGCACCAC CTTCATCGAG CAAAGTCGCC ACTACGCTGA TGTGCTGAGA 
AAGAAGGGCT ACAAGGCGAG CTTTACGCTC TTCAAAGGGT ACGACCACTT CGACATCATC 
GAGGAGACGG CCATCGACGA TTCGGATGTA TCCCGCTTTC TGCGCAACAT TGAAATTGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285676.1
CDS	206..1108
Translation	MYNPRCKDLDRDYFPSYHTTRFQDQPEPNLAVLEHFVRVTKQHGRELTEKQGITVDHLRYGEGRQLVDVFYSEKTTNQAPLFVFVHGGYWQEMDMSMSCSIVGPLVRRGYRVAVMDYNLCPQVTLEQLMTQFTHFLNWIFDYTEMTKVSSLTFAGHSAGAHLLAQILMRPNVITAQRSKMVWALIFLCGVYDLRELSNLESVNPKNILGLNERNIESVSPMLWEYTDVTVWNSTKIYVVAAEHDSTTFIEQSRHYADVLRKKGYKASFTLFKGYDHFDIIEETAIDDSDVSRFLRNIEIE

Target ORF information:

RefSeq Version	NM_001298747.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kynurenine formamidase, transcript variant B (KFase), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298747.1

ORF Insert Sequence:

ATGTACAATC CGAGGTGCAA AGACCTTGAC CGGGACTATT TTCCCAGCTA TCATACGACC 
AGGTTTCAGG ATCAGCCGGA ACCGAATTTG GCCGTGCTAG AGCACTTTGT TCGGGTGACC 
AAGCAGCATG GCAGGGAACT GACTGAGAAG CAGGGCATCA CTGTGGACCA CTTGCGCTAC 
GGAGAAGGTC GTCAGTTGGT GGATGTGTTC TACAGCGAAA AGACCACCAA CCAGGCGCCG 
CTTTTCGTCT TCGTGCACGG CGGCTACTGG CAGGAGATGG ACATGAGCAT GTCCTGCTCG 
ATTGTGGGTC CCCTGGTGCG GCGTGGATAC CGAGTGGCCG TCATGGACTA CAACCTGTGC 
CCGCAGGTCA CCCTCGAGCA GCTGATGACC CAGTTCACCC ATTTCCTCAA CTGGATCTTT 
GACTACACCG AAATGACCAA AGTGTCGTCG CTCACCTTCG CCGGACACTC GGCTGGTGCC 
CACTTGCTGG CCCAGATACT CATGCGCCCA AATGTGATTA CAGCCCAGCG GAGCAAGATG 
GTGTGGGCTT TGATCTTCCT CTGCGGCGTT TACGATCTGA GGGAGCTCTC TAACTTGGAA 
TCGGTTAATC CCAAAAATAT TCTGGGCCTC AACGAGCGGA ATATCGAGTC CGTGTCGCCC 
ATGCTGTGGG AATACACCGA TGTTACCGTT TGGAACTCCA CCAAGATCTA CGTGGTGGCC 
GCCGAGCATG ACAGCACCAC CTTCATCGAG CAAAGTCGCC ACTACGCTGA TGTGCTGAGA 
AAGAAGGGCT ACAAGGCGAG CTTTACGCTC TTCAAAGGGT ACGACCACTT CGACATCATC 
GAGGAGACGG CCATCGACGA TTCGGATGTA TCCCGCTTTC TGCGCAACAT TGAAATTGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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KFase cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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