gkt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	gkt
Entrez Gene ID	33530
Full Name	glaikit
Synonyms	CG8825,CG8826,Dmel\CG8825,Gkt,TDP1,Tdp1,anon-23Db,c22
General protein information	Preferred Names glaikit Names CG8825 gene product from transcript CG8825-RA CG8825-PA Tyrosyl-DNA phosophodiesterase 1 anon-23Db gkt-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 23D4-23D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_078741.4	NP_523465.2	glaikit

Pathways from BioSystems

Homologies

Homo sapiens (human)	TDP1	NP_001008744.1
Danio rerio (zebrafish)	tdp1	XP_700174.2
Xenopus tropicalis (tropical clawed frog)	tdp1	NP_001039242.1
Oryza sativa (rice)	Os07g0530100	NP_001059844.1
Bos taurus (cattle)	TDP1	NP_001180084.1
Gallus gallus (chicken)	TDP1	XP_004941916.1
Caenorhabditis elegans (roundworm)	F52C12.1	NP_500149.1
Macaca mulatta (Rhesus monkey)	TDP1	XP_001089545.1
Canis lupus familiaris (dog)	TDP1	XP_547950.2
Pan troglodytes (chimpanzee)	TDP1	NP_001233557.1
Mus musculus (house mouse)	Tdp1	NP_082630.2
Rattus norvegicus (Norway rat)	Tdp1	NP_001026827.1
Arabidopsis thaliana (thale cress)	TDP1	NP_197021.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010577	XP_558110.3
Drosophila melanogaster (fruit fly)	gkt	NP_523465.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gkt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gkt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13611	NM_078741.4 Latest version!	Drosophila melanogaster glaikit (gkt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gkt ( NM_078741.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523465.2 Drosophila melanogaster glaikit (gkt), mRNA.

Price & Availability↑

CloneID	OFa13611
Clone ID Related Accession (Same CDS sequence)	NM_078741.4
Accession Version	NM_078741.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	glaikit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078741.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGAGT GTCCTTATGG CGAAAAGTGC TACAGGAAGA ATCCCATTCA TTTTGGCGAG 
TTCTCCCATG CCCACCTTGA TGCCATTTAT GCGAAGGGAA ATGAATCGGG GGACTATGAG 
ATACCAGCCA ATTACTCCAG CGAGATGATT CACACCCAGT TGAAGCTGCT GGAGAAACTG 
TTTCCCAAGC AGGCAACCAA CAAGGAACAG GAAGCACATT CCTCTAGCTC AAAACCAGCC 
GTAACTGCTC CGGTTGCCAG TGGATCCTCG AGTTCTGGGT CCCTAGATAC CAATCCCAGT 
GGATCGTCAG CCTCCGGGCC AGCTGCTTCC CAAGACACCT CAAATCTGGC CAAAAAACAG 
AAGCTAAATG CCAAGAACAT CAGAGATTAT ATTCCTGTGG TAATCGAAAA GGGAGGAATG 
GCCAAAAAGC TGGAGCGGGC AGCTCCCTAT AACATGTTCC TCACCGCGAT CACGGACTCC 
AAGCCCACCC ACTCGGAGCC ATTGAGCATA ACGCTGCAGG AAATCCTGGA CGAAAGTCTC 
GGCGAGATTG AGAGCACCGT CCAAATTAAC TTTATGGTGG ACATTGGCTG GCTCCTGGGA 
CACTACTACT TTGCCGGGAT ACTGGACAAG CCACTTTTGC TGCTCTACGG CGACGAATCG 
CCGGAGCTGC TTAGCATTGG GAAGTTCAAG CAGCAGGTCA CGGCCATTCG TGTCAAGATG 
CCCACGCCGT TTGCCACCTC GCACACCAAG ATGATGTTCC TCGGCTACAG CGATGGCTCC 
ATGCGGGTGG TCATATCCAC GGCGAATCTG TACGAGGACG ACTGGCACAA TCGCACGCAG 
GGCCTGTGGA TCAGTCCCAA GCTGCCTGCT CTGCCGGTCG ATGCGGATAC GGGCGCTGGC 
GAAAGTCTGA CGGGCTTCAA ACAGGATCTG ATGCTGTACT TGGTGGAGTA CAAGATAAGC 
CAGCTGCAGC CTTGGATAGC AAGGATTCGG AACAGTGATT TCAGTGCCAT CAACGTTTTC 
TTCTTGGGCT CTGTGCCCGG AGGTCATCGC GAGGGCAGCG TGCGTGGTCA TCCATGGGGC 
CACGCCCGCT TGGCCTCCTT GCTCGCCAAA CACGCGGCGC CCATCGACGA TCGCATACCG 
GTCGTCTGCC AGAGCTCGAG CATCGGCAGC CTGGGAGCCA ACGTGCAGGC CTGGATTCAG 
CAGGATTTCG TCAATAGCCT GAAGAAGGAT TCCACGCCTG TGGGCAAGCT GCGCCAGATG 
CCGCCCTTCA AGATGATCTA TCCCAGCTAC GGCAACGTCG CGGGCAGTCA TGATGGAATG 
CTGGGCGGCG GGTGTTTGCC CTACGGCAAG AACACCAACG ATAAGCAGCC ATGGCTCAAG 
GACTATCTGC AGCAATGGAA GTCCAGCGAT CGCTTTCGCT CGCGGGCAAT GCCGCACATC 
AAGAGCTATA CGCGCTTCAA TCTGGAGGAT CAGTCGGTGT ACTGGTTCGT TTTAACCTCG 
GCGAATCTCT CGAAGGCAGC CTGGGGCTGC TTTAACAAAA ACTCGAATAT CCAACCCTGC 
CTGAGGATCG CCAACTATGA GGCTGGCGTC CTATTCCTGC CCAGATTTGT GACTGGCGAG 
GACACCTTTC CTTTGGGTAA CAATCGTGAT GGAGTGCCAG CGTTTCCGCT ACCATACGAC 
GTTCCATTGA CGCCCTATGC TCCAGATGAT AAGCCCTTCC TGATGGATTA CCTACAAGGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523465.2
CDS	124..1866
Translation	MKECPYGEKCYRKNPIHFGEFSHAHLDAIYAKGNESGDYEIPANYSSEMIHTQLKLLEKLFPKQATNKEQEAHSSSSKPAVTAPVASGSSSSGSLDTNPSGSSASGPAASQDTSNLAKKQKLNAKNIRDYIPVVIEKGGMAKKLERAAPYNMFLTAITDSKPTHSEPLSITLQEILDESLGEIESTVQINFMVDIGWLLGHYYFAGILDKPLLLLYGDESPELLSIGKFKQQVTAIRVKMPTPFATSHTKMMFLGYSDGSMRVVISTANLYEDDWHNRTQGLWISPKLPALPVDADTGAGESLTGFKQDLMLYLVEYKISQLQPWIARIRNSDFSAINVFFLGSVPGGHREGSVRGHPWGHARLASLLAKHAAPIDDRIPVVCQSSSIGSLGANVQAWIQQDFVNSLKKDSTPVGKLRQMPPFKMIYPSYGNVAGSHDGMLGGGCLPYGKNTNDKQPWLKDYLQQWKSSDRFRSRAMPHIKSYTRFNLEDQSVYWFVLTSANLSKAAWGCFNKNSNIQPCLRIANYEAGVLFLPRFVTGEDTFPLGNNRDGVPAFPLPYDVPLTPYAPDDKPFLMDYLQG

Target ORF information:

RefSeq Version	NM_078741.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glaikit (gkt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078741.4

ORF Insert Sequence:

ATGAAGGAGT GTCCTTATGG CGAAAAGTGC TACAGGAAGA ATCCCATTCA TTTTGGCGAG 
TTCTCCCATG CCCACCTTGA TGCCATTTAT GCGAAGGGAA ATGAATCGGG GGACTATGAG 
ATACCAGCCA ATTACTCCAG CGAGATGATT CACACCCAGT TGAAGCTGCT GGAGAAACTG 
TTTCCCAAGC AGGCAACCAA CAAGGAACAG GAAGCACATT CCTCTAGCTC AAAACCAGCC 
GTAACTGCTC CGGTTGCCAG TGGATCCTCG AGTTCTGGGT CCCTAGATAC CAATCCCAGT 
GGATCGTCAG CCTCCGGGCC AGCTGCTTCC CAAGACACCT CAAATCTGGC CAAAAAACAG 
AAGCTAAATG CCAAGAACAT CAGAGATTAT ATTCCTGTGG TAATCGAAAA GGGAGGAATG 
GCCAAAAAGC TGGAGCGGGC AGCTCCCTAT AACATGTTCC TCACCGCGAT CACGGACTCC 
AAGCCCACCC ACTCGGAGCC ATTGAGCATA ACGCTGCAGG AAATCCTGGA CGAAAGTCTC 
GGCGAGATTG AGAGCACCGT CCAAATTAAC TTTATGGTGG ACATTGGCTG GCTCCTGGGA 
CACTACTACT TTGCCGGGAT ACTGGACAAG CCACTTTTGC TGCTCTACGG CGACGAATCG 
CCGGAGCTGC TTAGCATTGG GAAGTTCAAG CAGCAGGTCA CGGCCATTCG TGTCAAGATG 
CCCACGCCGT TTGCCACCTC GCACACCAAG ATGATGTTCC TCGGCTACAG CGATGGCTCC 
ATGCGGGTGG TCATATCCAC GGCGAATCTG TACGAGGACG ACTGGCACAA TCGCACGCAG 
GGCCTGTGGA TCAGTCCCAA GCTGCCTGCT CTGCCGGTCG ATGCGGATAC GGGCGCTGGC 
GAAAGTCTGA CGGGCTTCAA ACAGGATCTG ATGCTGTACT TGGTGGAGTA CAAGATAAGC 
CAGCTGCAGC CTTGGATAGC AAGGATTCGG AACAGTGATT TCAGTGCCAT CAACGTTTTC 
TTCTTGGGCT CTGTGCCCGG AGGTCATCGC GAGGGCAGCG TGCGTGGTCA TCCATGGGGC 
CACGCCCGCT TGGCCTCCTT GCTCGCCAAA CACGCGGCGC CCATCGACGA TCGCATACCG 
GTCGTCTGCC AGAGCTCGAG CATCGGCAGC CTGGGAGCCA ACGTGCAGGC CTGGATTCAG 
CAGGATTTCG TCAATAGCCT GAAGAAGGAT TCCACGCCTG TGGGCAAGCT GCGCCAGATG 
CCGCCCTTCA AGATGATCTA TCCCAGCTAC GGCAACGTCG CGGGCAGTCA TGATGGAATG 
CTGGGCGGCG GGTGTTTGCC CTACGGCAAG AACACCAACG ATAAGCAGCC ATGGCTCAAG 
GACTATCTGC AGCAATGGAA GTCCAGCGAT CGCTTTCGCT CGCGGGCAAT GCCGCACATC 
AAGAGCTATA CGCGCTTCAA TCTGGAGGAT CAGTCGGTGT ACTGGTTCGT TTTAACCTCG 
GCGAATCTCT CGAAGGCAGC CTGGGGCTGC TTTAACAAAA ACTCGAATAT CCAACCCTGC 
CTGAGGATCG CCAACTATGA GGCTGGCGTC CTATTCCTGC CCAGATTTGT GACTGGCGAG 
GACACCTTTC CTTTGGGTAA CAATCGTGAT GGAGTGCCAG CGTTTCCGCT ACCATACGAC 
GTTCCATTGA CGCCCTATGC TCCAGATGAT AAGCCCTTCC TGATGGATTA CCTACAAGGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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gkt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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