Gs1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gs1
Entrez Gene ID	33172
Full Name	Glutamine synthetase 1
Synonyms	CG2718,Dmel\CG2718,GS1,GSI,GsI,fs(2)P52,fs(2)throng,gs-m,gsI,l(2)21Bc,mt-gs,thg
General protein information	Preferred Names Glutamine synthetase 1 Names CG2718 gene product from transcript CG2718-RC CG2718-PB CG2718-PC CG2718-PD Glutamate-ammonia ligase Gs1-PB Gs1-PC Gs1-PD glutamine synthase 1 glutamine synthetase glutamine synthetase 1 glutamine synthetase I glutamine synthetase-1 mitochondrial glutamine synthetase throng
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21B2-21B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169368.3	NP_001162839.1	glutamine synthetase 1, isoform D
NM_057222.4	NP_476570.1	glutamine synthetase 1, isoform B
NM_164367.2	NP_722606.1	glutamine synthetase 1, isoform C

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_06888	XP_003709618.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008988	XP_319738.2
Mus musculus (house mouse)	Glul	NP_032157.2
Rattus norvegicus (Norway rat)	Glul	NP_058769.4
Danio rerio (zebrafish)	glulb	NP_878286.2
Drosophila melanogaster (fruit fly)	Gs1	NP_001162839.1
Bos taurus (cattle)	GLUL	NP_001035564.1
Schizosaccharomyces pombe (fission yeast)	gln1	NP_593400.1
Oryza sativa (rice)	Os03g0223400	NP_001049424.1
Neurospora crassa	NCU06724	XP_960904.2
Pan troglodytes (chimpanzee)	GLUL	XP_001160939.1
Arabidopsis thaliana (thale cress)	GSR 1	NP_198576.1
Arabidopsis thaliana (thale cress)	GSR2	NP_176794.1
Xenopus tropicalis (tropical clawed frog)	glul	XP_002944531.2
Gallus gallus (chicken)	GLUL	NP_990824.1
Kluyveromyces lactis	KLLA0E06293g	XP_454231.1
Macaca mulatta (Rhesus monkey)	GLUL	XP_001114827.1
Canis lupus familiaris (dog)	GLUL	NP_001002965.1
Caenorhabditis elegans (roundworm)	gln-1	NP_509012.2
Saccharomyces cerevisiae (baker's yeast)	GLN1	NP_015360.2
Arabidopsis thaliana (thale cress)	GLN1;4	NP_568335.1
Magnaporthe oryzae (rice blast fungus)	MGG_14279	XP_003719336.1
Eremothecium gossypii	AGOS_ACR182C	NP_983584.1
Homo sapiens (human)	GLUL	NP_002056.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13305	NM_057222.4 Latest version!	Drosophila melanogaster glutamine synthetase 1, transcript variant B (Gs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa13305	NM_164367.2 Latest version!	Drosophila melanogaster glutamine synthetase 1, transcript variant C (Gs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa13305	NM_001169368.3 Latest version!	Drosophila melanogaster glutamine synthetase 1, transcript variant D (Gs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gs1 ( NM_001169368.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162839.1 Drosophila melanogaster glutamine synthetase 1, transcript variant D (Gs1), mRNA.

Price & Availability↑

CloneID	OFa13305
Clone ID Related Accession (Same CDS sequence)	NM_001169368.3 , NM_057222.4 , NM_164367.2
Accession Version	NM_001169368.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	glutamine synthetase 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001169368.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCACTAC GCGTGGCAGG ACTCTTCCTG AAGAAGGAGC TGGTGGCTCC CGCAACACAG 
CAGCTGCGTC TGCTGCGTAC TGGCAATACC ACCCGCTCCC AGTTCTTGGC CAACTCCCCC 
AACACGGCTC TAGACAAGAG CATTCTGCAG AGGTACCGCA ACCTGGAAAC GCCGGCCAAT 
CGAGTGCAGG CTACTTACTT ATGGATCGAC GGTACCGGCG AGAACATTCG CCTCAAGGAT 
CGCGTTTTGG ACAAAGTACC CAGCTCCGTG GAGGACTTGC CGGATTGGCA GTACGACGGC 
AGTTCCACCT ACCAGGCCCA TGGCGAGAAC TCGGACACCA CGCTCAAGCC ACGCGCCATA 
TATCGCGATC CCTTTAAGCC GGGAAAGAAC GACGTAATTG TCCTGTGCGA CACCTACAGC 
GCCGACGGCA AGCCGACGGC GTCCAACAAG CGTGCCGCGT TCCAGGCAGC CATTGACCTA 
ATTAGCGATC AGGAGCCCTG GTTCGGCATT GAGCAAGAAT ACACTTTATT AGACGTGGAC 
GGACGTCCCT TCGGCTGGCC GGAGAACGGT TTCCCAGCCC CACAAGGTCC TTACTACTGC 
GGCGTGGGAG CTGACCGTGT GTACGCTCGC GATCTTGTGG AGGCCCACGT CGTGGCCTGC 
CTGTATGCTG GGATCGACTT CGCCGGCACC AATGCCGAGG TAATGCCCGC GCAGTGGGAG 
TTCCAAATTG GTCCAGCCGG GATTAAGGCA TGTGACGATT TATGGGTGTC GCGATATATT 
TTGCAGCGCA TAGCTGAGGA GTATGGCGTA GTGGTCACCT TTGACCCCAA ACCGATGGAG 
GGTCAGTGGA ACGGAGCCGG CGCGCATACC AACTTTTCCA CAAAGGAGAT GCGTGCTGAT 
GGCGGAATCA AGGCCATCGA GGAAGCTATC GAGAAGCTGA GCAAGCGCCA CGAGCGGCAC 
ATCAAGGCAT ACGACCCCAA GGAAGGCAAA GACAATGAAC GGCGCCTCGT GGGACGTCTT 
GAGACTTCTA GCATCGATAA GTTTTCTTGG GGCGTGGCCA ACCGGGCCGT AAGTGTGCGA 
GTGCCGCGTG GCGTTGCGAC GGCCGGCAAA GGATACCTCG AAGACCGGCG GCCAAGCTCC 
AACTGTGATC CCTACGCCGT GTGCAACGCC ATTGTTCGCA CCTGCCTGCT CAACGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162839.1
CDS	187..1386
Translation	MALRVAGLFLKKELVAPATQQLRLLRTGNTTRSQFLANSPNTALDKSILQRYRNLETPANRVQATYLWIDGTGENIRLKDRVLDKVPSSVEDLPDWQYDGSSTYQAHGENSDTTLKPRAIYRDPFKPGKNDVIVLCDTYSADGKPTASNKRAAFQAAIDLISDQEPWFGIEQEYTLLDVDGRPFGWPENGFPAPQGPYYCGVGADRVYARDLVEAHVVACLYAGIDFAGTNAEVMPAQWEFQIGPAGIKACDDLWVSRYILQRIAEEYGVVVTFDPKPMEGQWNGAGAHTNFSTKEMRADGGIKAIEEAIEKLSKRHERHIKAYDPKEGKDNERRLVGRLETSSIDKFSWGVANRAVSVRVPRGVATAGKGYLEDRRPSSNCDPYAVCNAIVRTCLLNE

Target ORF information:

RefSeq Version	NM_001169368.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glutamine synthetase 1, transcript variant D (Gs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169368.3

ORF Insert Sequence:

ATGGCACTAC GCGTGGCAGG ACTCTTCCTG AAGAAGGAGC TGGTGGCTCC CGCAACACAG 
CAGCTGCGTC TGCTGCGTAC TGGCAATACC ACCCGCTCCC AGTTCTTGGC CAACTCCCCC 
AACACGGCTC TAGACAAGAG CATTCTGCAG AGGTACCGCA ACCTGGAAAC GCCGGCCAAT 
CGAGTGCAGG CTACTTACTT ATGGATCGAC GGTACCGGCG AGAACATTCG CCTCAAGGAT 
CGCGTTTTGG ACAAAGTACC CAGCTCCGTG GAGGACTTGC CGGATTGGCA GTACGACGGC 
AGTTCCACCT ACCAGGCCCA TGGCGAGAAC TCGGACACCA CGCTCAAGCC ACGCGCCATA 
TATCGCGATC CCTTTAAGCC GGGAAAGAAC GACGTAATTG TCCTGTGCGA CACCTACAGC 
GCCGACGGCA AGCCGACGGC GTCCAACAAG CGTGCCGCGT TCCAGGCAGC CATTGACCTA 
ATTAGCGATC AGGAGCCCTG GTTCGGCATT GAGCAAGAAT ACACTTTATT AGACGTGGAC 
GGACGTCCCT TCGGCTGGCC GGAGAACGGT TTCCCAGCCC CACAAGGTCC TTACTACTGC 
GGCGTGGGAG CTGACCGTGT GTACGCTCGC GATCTTGTGG AGGCCCACGT CGTGGCCTGC 
CTGTATGCTG GGATCGACTT CGCCGGCACC AATGCCGAGG TAATGCCCGC GCAGTGGGAG 
TTCCAAATTG GTCCAGCCGG GATTAAGGCA TGTGACGATT TATGGGTGTC GCGATATATT 
TTGCAGCGCA TAGCTGAGGA GTATGGCGTA GTGGTCACCT TTGACCCCAA ACCGATGGAG 
GGTCAGTGGA ACGGAGCCGG CGCGCATACC AACTTTTCCA CAAAGGAGAT GCGTGCTGAT 
GGCGGAATCA AGGCCATCGA GGAAGCTATC GAGAAGCTGA GCAAGCGCCA CGAGCGGCAC 
ATCAAGGCAT ACGACCCCAA GGAAGGCAAA GACAATGAAC GGCGCCTCGT GGGACGTCTT 
GAGACTTCTA GCATCGATAA GTTTTCTTGG GGCGTGGCCA ACCGGGCCGT AAGTGTGCGA 
GTGCCGCGTG GCGTTGCGAC GGCCGGCAAA GGATACCTCG AAGACCGGCG GCCAAGCTCC 
AACTGTGATC CCTACGCCGT GTGCAACGCC ATTGTTCGCA CCTGCCTGCT CAACGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gs1 ( NM_057222.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476570.1 Drosophila melanogaster glutamine synthetase 1, transcript variant B (Gs1), mRNA.

Price & Availability↑

CloneID	OFa13305
Clone ID Related Accession (Same CDS sequence)	NM_001169368.3 , NM_057222.4 , NM_164367.2
Accession Version	NM_057222.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	glutamine synthetase 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057222.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCACTAC GCGTGGCAGG ACTCTTCCTG AAGAAGGAGC TGGTGGCTCC CGCAACACAG 
CAGCTGCGTC TGCTGCGTAC TGGCAATACC ACCCGCTCCC AGTTCTTGGC CAACTCCCCC 
AACACGGCTC TAGACAAGAG CATTCTGCAG AGGTACCGCA ACCTGGAAAC GCCGGCCAAT 
CGAGTGCAGG CTACTTACTT ATGGATCGAC GGTACCGGCG AGAACATTCG CCTCAAGGAT 
CGCGTTTTGG ACAAAGTACC CAGCTCCGTG GAGGACTTGC CGGATTGGCA GTACGACGGC 
AGTTCCACCT ACCAGGCCCA TGGCGAGAAC TCGGACACCA CGCTCAAGCC ACGCGCCATA 
TATCGCGATC CCTTTAAGCC GGGAAAGAAC GACGTAATTG TCCTGTGCGA CACCTACAGC 
GCCGACGGCA AGCCGACGGC GTCCAACAAG CGTGCCGCGT TCCAGGCAGC CATTGACCTA 
ATTAGCGATC AGGAGCCCTG GTTCGGCATT GAGCAAGAAT ACACTTTATT AGACGTGGAC 
GGACGTCCCT TCGGCTGGCC GGAGAACGGT TTCCCAGCCC CACAAGGTCC TTACTACTGC 
GGCGTGGGAG CTGACCGTGT GTACGCTCGC GATCTTGTGG AGGCCCACGT CGTGGCCTGC 
CTGTATGCTG GGATCGACTT CGCCGGCACC AATGCCGAGG TAATGCCCGC GCAGTGGGAG 
TTCCAAATTG GTCCAGCCGG GATTAAGGCA TGTGACGATT TATGGGTGTC GCGATATATT 
TTGCAGCGCA TAGCTGAGGA GTATGGCGTA GTGGTCACCT TTGACCCCAA ACCGATGGAG 
GGTCAGTGGA ACGGAGCCGG CGCGCATACC AACTTTTCCA CAAAGGAGAT GCGTGCTGAT 
GGCGGAATCA AGGCCATCGA GGAAGCTATC GAGAAGCTGA GCAAGCGCCA CGAGCGGCAC 
ATCAAGGCAT ACGACCCCAA GGAAGGCAAA GACAATGAAC GGCGCCTCGT GGGACGTCTT 
GAGACTTCTA GCATCGATAA GTTTTCTTGG GGCGTGGCCA ACCGGGCCGT AAGTGTGCGA 
GTGCCGCGTG GCGTTGCGAC GGCCGGCAAA GGATACCTCG AAGACCGGCG GCCAAGCTCC 
AACTGTGATC CCTACGCCGT GTGCAACGCC ATTGTTCGCA CCTGCCTGCT CAACGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476570.1
CDS	187..1386
Translation	MALRVAGLFLKKELVAPATQQLRLLRTGNTTRSQFLANSPNTALDKSILQRYRNLETPANRVQATYLWIDGTGENIRLKDRVLDKVPSSVEDLPDWQYDGSSTYQAHGENSDTTLKPRAIYRDPFKPGKNDVIVLCDTYSADGKPTASNKRAAFQAAIDLISDQEPWFGIEQEYTLLDVDGRPFGWPENGFPAPQGPYYCGVGADRVYARDLVEAHVVACLYAGIDFAGTNAEVMPAQWEFQIGPAGIKACDDLWVSRYILQRIAEEYGVVVTFDPKPMEGQWNGAGAHTNFSTKEMRADGGIKAIEEAIEKLSKRHERHIKAYDPKEGKDNERRLVGRLETSSIDKFSWGVANRAVSVRVPRGVATAGKGYLEDRRPSSNCDPYAVCNAIVRTCLLNE

Target ORF information:

RefSeq Version	NM_057222.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glutamine synthetase 1, transcript variant B (Gs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057222.4

ORF Insert Sequence:

ATGGCACTAC GCGTGGCAGG ACTCTTCCTG AAGAAGGAGC TGGTGGCTCC CGCAACACAG 
CAGCTGCGTC TGCTGCGTAC TGGCAATACC ACCCGCTCCC AGTTCTTGGC CAACTCCCCC 
AACACGGCTC TAGACAAGAG CATTCTGCAG AGGTACCGCA ACCTGGAAAC GCCGGCCAAT 
CGAGTGCAGG CTACTTACTT ATGGATCGAC GGTACCGGCG AGAACATTCG CCTCAAGGAT 
CGCGTTTTGG ACAAAGTACC CAGCTCCGTG GAGGACTTGC CGGATTGGCA GTACGACGGC 
AGTTCCACCT ACCAGGCCCA TGGCGAGAAC TCGGACACCA CGCTCAAGCC ACGCGCCATA 
TATCGCGATC CCTTTAAGCC GGGAAAGAAC GACGTAATTG TCCTGTGCGA CACCTACAGC 
GCCGACGGCA AGCCGACGGC GTCCAACAAG CGTGCCGCGT TCCAGGCAGC CATTGACCTA 
ATTAGCGATC AGGAGCCCTG GTTCGGCATT GAGCAAGAAT ACACTTTATT AGACGTGGAC 
GGACGTCCCT TCGGCTGGCC GGAGAACGGT TTCCCAGCCC CACAAGGTCC TTACTACTGC 
GGCGTGGGAG CTGACCGTGT GTACGCTCGC GATCTTGTGG AGGCCCACGT CGTGGCCTGC 
CTGTATGCTG GGATCGACTT CGCCGGCACC AATGCCGAGG TAATGCCCGC GCAGTGGGAG 
TTCCAAATTG GTCCAGCCGG GATTAAGGCA TGTGACGATT TATGGGTGTC GCGATATATT 
TTGCAGCGCA TAGCTGAGGA GTATGGCGTA GTGGTCACCT TTGACCCCAA ACCGATGGAG 
GGTCAGTGGA ACGGAGCCGG CGCGCATACC AACTTTTCCA CAAAGGAGAT GCGTGCTGAT 
GGCGGAATCA AGGCCATCGA GGAAGCTATC GAGAAGCTGA GCAAGCGCCA CGAGCGGCAC 
ATCAAGGCAT ACGACCCCAA GGAAGGCAAA GACAATGAAC GGCGCCTCGT GGGACGTCTT 
GAGACTTCTA GCATCGATAA GTTTTCTTGG GGCGTGGCCA ACCGGGCCGT AAGTGTGCGA 
GTGCCGCGTG GCGTTGCGAC GGCCGGCAAA GGATACCTCG AAGACCGGCG GCCAAGCTCC 
AACTGTGATC CCTACGCCGT GTGCAACGCC ATTGTTCGCA CCTGCCTGCT CAACGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gs1 ( NM_164367.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722606.1 Drosophila melanogaster glutamine synthetase 1, transcript variant C (Gs1), mRNA.

Price & Availability↑

CloneID	OFa13305
Clone ID Related Accession (Same CDS sequence)	NM_001169368.3 , NM_057222.4 , NM_164367.2
Accession Version	NM_164367.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	glutamine synthetase 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_164367.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCACTAC GCGTGGCAGG ACTCTTCCTG AAGAAGGAGC TGGTGGCTCC CGCAACACAG 
CAGCTGCGTC TGCTGCGTAC TGGCAATACC ACCCGCTCCC AGTTCTTGGC CAACTCCCCC 
AACACGGCTC TAGACAAGAG CATTCTGCAG AGGTACCGCA ACCTGGAAAC GCCGGCCAAT 
CGAGTGCAGG CTACTTACTT ATGGATCGAC GGTACCGGCG AGAACATTCG CCTCAAGGAT 
CGCGTTTTGG ACAAAGTACC CAGCTCCGTG GAGGACTTGC CGGATTGGCA GTACGACGGC 
AGTTCCACCT ACCAGGCCCA TGGCGAGAAC TCGGACACCA CGCTCAAGCC ACGCGCCATA 
TATCGCGATC CCTTTAAGCC GGGAAAGAAC GACGTAATTG TCCTGTGCGA CACCTACAGC 
GCCGACGGCA AGCCGACGGC GTCCAACAAG CGTGCCGCGT TCCAGGCAGC CATTGACCTA 
ATTAGCGATC AGGAGCCCTG GTTCGGCATT GAGCAAGAAT ACACTTTATT AGACGTGGAC 
GGACGTCCCT TCGGCTGGCC GGAGAACGGT TTCCCAGCCC CACAAGGTCC TTACTACTGC 
GGCGTGGGAG CTGACCGTGT GTACGCTCGC GATCTTGTGG AGGCCCACGT CGTGGCCTGC 
CTGTATGCTG GGATCGACTT CGCCGGCACC AATGCCGAGG TAATGCCCGC GCAGTGGGAG 
TTCCAAATTG GTCCAGCCGG GATTAAGGCA TGTGACGATT TATGGGTGTC GCGATATATT 
TTGCAGCGCA TAGCTGAGGA GTATGGCGTA GTGGTCACCT TTGACCCCAA ACCGATGGAG 
GGTCAGTGGA ACGGAGCCGG CGCGCATACC AACTTTTCCA CAAAGGAGAT GCGTGCTGAT 
GGCGGAATCA AGGCCATCGA GGAAGCTATC GAGAAGCTGA GCAAGCGCCA CGAGCGGCAC 
ATCAAGGCAT ACGACCCCAA GGAAGGCAAA GACAATGAAC GGCGCCTCGT GGGACGTCTT 
GAGACTTCTA GCATCGATAA GTTTTCTTGG GGCGTGGCCA ACCGGGCCGT AAGTGTGCGA 
GTGCCGCGTG GCGTTGCGAC GGCCGGCAAA GGATACCTCG AAGACCGGCG GCCAAGCTCC 
AACTGTGATC CCTACGCCGT GTGCAACGCC ATTGTTCGCA CCTGCCTGCT CAACGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722606.1
CDS	407..1606
Translation	MALRVAGLFLKKELVAPATQQLRLLRTGNTTRSQFLANSPNTALDKSILQRYRNLETPANRVQATYLWIDGTGENIRLKDRVLDKVPSSVEDLPDWQYDGSSTYQAHGENSDTTLKPRAIYRDPFKPGKNDVIVLCDTYSADGKPTASNKRAAFQAAIDLISDQEPWFGIEQEYTLLDVDGRPFGWPENGFPAPQGPYYCGVGADRVYARDLVEAHVVACLYAGIDFAGTNAEVMPAQWEFQIGPAGIKACDDLWVSRYILQRIAEEYGVVVTFDPKPMEGQWNGAGAHTNFSTKEMRADGGIKAIEEAIEKLSKRHERHIKAYDPKEGKDNERRLVGRLETSSIDKFSWGVANRAVSVRVPRGVATAGKGYLEDRRPSSNCDPYAVCNAIVRTCLLNE

Target ORF information:

RefSeq Version	NM_164367.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glutamine synthetase 1, transcript variant C (Gs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164367.2

ORF Insert Sequence:

ATGGCACTAC GCGTGGCAGG ACTCTTCCTG AAGAAGGAGC TGGTGGCTCC CGCAACACAG 
CAGCTGCGTC TGCTGCGTAC TGGCAATACC ACCCGCTCCC AGTTCTTGGC CAACTCCCCC 
AACACGGCTC TAGACAAGAG CATTCTGCAG AGGTACCGCA ACCTGGAAAC GCCGGCCAAT 
CGAGTGCAGG CTACTTACTT ATGGATCGAC GGTACCGGCG AGAACATTCG CCTCAAGGAT 
CGCGTTTTGG ACAAAGTACC CAGCTCCGTG GAGGACTTGC CGGATTGGCA GTACGACGGC 
AGTTCCACCT ACCAGGCCCA TGGCGAGAAC TCGGACACCA CGCTCAAGCC ACGCGCCATA 
TATCGCGATC CCTTTAAGCC GGGAAAGAAC GACGTAATTG TCCTGTGCGA CACCTACAGC 
GCCGACGGCA AGCCGACGGC GTCCAACAAG CGTGCCGCGT TCCAGGCAGC CATTGACCTA 
ATTAGCGATC AGGAGCCCTG GTTCGGCATT GAGCAAGAAT ACACTTTATT AGACGTGGAC 
GGACGTCCCT TCGGCTGGCC GGAGAACGGT TTCCCAGCCC CACAAGGTCC TTACTACTGC 
GGCGTGGGAG CTGACCGTGT GTACGCTCGC GATCTTGTGG AGGCCCACGT CGTGGCCTGC 
CTGTATGCTG GGATCGACTT CGCCGGCACC AATGCCGAGG TAATGCCCGC GCAGTGGGAG 
TTCCAAATTG GTCCAGCCGG GATTAAGGCA TGTGACGATT TATGGGTGTC GCGATATATT 
TTGCAGCGCA TAGCTGAGGA GTATGGCGTA GTGGTCACCT TTGACCCCAA ACCGATGGAG 
GGTCAGTGGA ACGGAGCCGG CGCGCATACC AACTTTTCCA CAAAGGAGAT GCGTGCTGAT 
GGCGGAATCA AGGCCATCGA GGAAGCTATC GAGAAGCTGA GCAAGCGCCA CGAGCGGCAC 
ATCAAGGCAT ACGACCCCAA GGAAGGCAAA GACAATGAAC GGCGCCTCGT GGGACGTCTT 
GAGACTTCTA GCATCGATAA GTTTTCTTGG GGCGTGGCCA ACCGGGCCGT AAGTGTGCGA 
GTGCCGCGTG GCGTTGCGAC GGCCGGCAAA GGATACCTCG AAGACCGGCG GCCAAGCTCC 
AACTGTGATC CCTACGCCGT GTGCAACGCC ATTGTTCGCA CCTGCCTGCT CAACGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
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Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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