Sk2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sk2
Entrez Gene ID	326219
Full Name	Sphingosine kinase 2
Synonyms	CG2159,CG32484,Dmel\CG32484,SK2,Sphk2,sk2
General protein information	Preferred Names Sphingosine kinase 2 Names CG32484 gene product from transcript CG32484-RA CG32484-PA Sk2-PA sphingosine kinase sphingosine kinase 2 sphingosine kinase II
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 63A3-63A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_139505.4	NP_647762.3	sphingosine kinase 2

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	LOC710441	XP_001099066.2
Bos taurus (cattle)	SPHK1	XP_002696250.3
Xenopus tropicalis (tropical clawed frog)	sphk1	NP_001016918.1
Gallus gallus (chicken)	SPHK1	XP_004946176.1
Pan troglodytes (chimpanzee)	SPHK1	XP_003953242.1
Canis lupus familiaris (dog)	SPHK1	XP_540448.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006995	XP_308775.3
Homo sapiens (human)	SPHK1	NP_892010.2
Mus musculus (house mouse)	Sphk1	NP_079643.2
Rattus norvegicus (Norway rat)	Sphk1	NP_001257740.1
Drosophila melanogaster (fruit fly)	Sk2	NP_647762.3
Danio rerio (zebrafish)	LOC100331840	XP_002667585.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19602	NM_139505.4 Latest version!	Drosophila melanogaster sphingosine kinase 2 (Sk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sk2 ( NM_139505.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_647762.3 Drosophila melanogaster sphingosine kinase 2 (Sk2), mRNA.

Price & Availability↑

CloneID	OFa19602
Clone ID Related Accession (Same CDS sequence)	NM_139505.4
Accession Version	NM_139505.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	sphingosine kinase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139505.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGAAT CTCTTGATAA GACCACCAGC CCGAGCTCGG CCAGTTCCAG GGCCACGCCC 
CCGGGAACGC AGGATGCGGA TGAGGGCCAC GACGTGAGCG ATACCTTCTA CACGAGCCAG 
CGCAAGAAGG GCAGCCACGT ATTTCGGGTG CGCCTTGACG CCACAGGATT CACCCTGCAG 
CGGGAGTCGC CGGGCGGTAG CATTGTTAAG GAGCAACATG TCCGCATATC GGACATTGTG 
GGTGCCCGCT GCATGCGGCC CAAGAAGAGC CGGCGCCTGG CGATGTCGGG CGCCTGTGCG 
TGCAGCTCCG GTAATCCCAA TTCGCCAGCC ATCTCGGCGT CCGGCGATCA CCATCGCCCT 
GCCACCACAC CAAGCAAATG CAGCACCAAT AGTCGGGATA ATATTCCTTC GGATGGCGGC 
GATGTCAGCG CGTTTCTCTA CGTTTTTGCC TATGTTCTGA AGAAGAGGAG CCTGCGGTCG 
GAGTTGCACC GGGAGCGAAC GGTGCTCACT CTGCGCTTCC GGTCGTTCGA CACCTTCGAG 
GACAACATGA GGGAGGCGGA TCGTTGGTAC AGATCCCTTC GCTGGCAGTT GCATCGCACG 
CTGGAGGAGA TCTTTGTGGC GCCGACGGTG GATGAGCGAC GCCGTCGAGT GCTTGTGCTG 
TTGAATCCCA AATCCGGTTC CGGTGACGCT CGTGAGGTCT TCAACATGCA CGTGACGCCG 
GTGCTCAACG AGGCCGAGGT GCCCTACGAC CTGTATGTAA CCAAGCATTC CAACTTTGCC 
ATCGAGTTCT TGAGCACCAG GTGCCTGGAC GCCTGGTGCT GCGTGGTGGC TGTCGGCGGA 
GACGGTCTCT TCCACGAGAT AGTCAATGGA CTGCTGCAGC GCCAGGACTG GGCCCACGTC 
CTGCCTCATC TGGCACTGGG AATCATTCCT TGCGGCTCCG GAAATGGATT GGCCCGCTCC 
ATTGCCCATT GTTACAACGA ACCATACTTC AGCAAGCCAG TGCTAGGAGC TGCTCTGACC 
GTAATCAGTG GACGCAGTTC ACCCATGGAC GTGGTCCGGG TGCAGCTGCA GAGTCGCTCC 
CTCTACTCCT TCCTGTCCAT CGGCTGGGGT CTGATCTCGG ACGTGGACAT CGAGAGCGAG 
CGCATTCGCA TGTTGGGCTA CCAGCGCTTC ACCGTGTGGA CCCTCTACCG TCTGGTGAAT 
CTGCGCACCT ACAACGGCCG AATCAGCTAT CTTCTGACGG ACCATGAGGT GTCCTCAACC 
CATAGCGCTA CCGGTTATGC TGCCCAGCGG AGAATGCAGA GCAGCCGTAG CTGCAACACG 
CACATCGACA TGCTAAATGG GCCGGCGCCC ATCTATCATT CCAGTGCCGA GTACCTGCCA 
CAGGAGTTTG CGGACGTGAT CTCCCTGGAG ACGTCCATCA ATCAGTCGTT CCGCTCGAGG 
TGCGACAGCT GGTTGTCGGG GGGATCGCGG CGCAGCTTTT ACTATTCCAT ATCGGAGAGC 
ATCTACCACA GTCTGGCGGA TGAGAGCGAG TTCGCCGGCC TGGCGGCCGC CTCGCTGGAA 
AACCGGCAGC AGAACTACGG TCCGGCAAGC GAGCTGCCGG ATCTGAACGA ACCGCTGTCC 
GAGGATCAGG GTTGGCTGGT GGAGGAGGGC GAGTTCGTCA TGATGCACGC CGTTTACCAG 
ACCCATCTGG GCATCGACTG TCATTTTGCG CCCAAGGCCC AGCTGAACGA CGGCACCATC 
TACCTGATCC TCATACGCGC CGGCATCAGC CGCCCGCACC TGCTGAGCTT CCTCTACAAC 
ATGAGCTCCG GCACTCACCT GCCGGAGTCG CACGACGACC ATGTGAAGGT GCTGCCAGTG 
CGAGCATTCC GCCTGGAGCC CTACGACAAT CACGGCATCA TCACGGTCGA CGGCGAGCGC 
GTCGAGTTCG GGCCCCTCCA AGCTGAGGTC CTGCCGGGCA TAGCCCGCGT CATGGTGCCC 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647762.3
CDS	104..2089
Translation	MSESLDKTTSPSSASSRATPPGTQDADEGHDVSDTFYTSQRKKGSHVFRVRLDATGFTLQRESPGGSIVKEQHVRISDIVGARCMRPKKSRRLAMSGACACSSGNPNSPAISASGDHHRPATTPSKCSTNSRDNIPSDGGDVSAFLYVFAYVLKKRSLRSELHRERTVLTLRFRSFDTFEDNMREADRWYRSLRWQLHRTLEEIFVAPTVDERRRRVLVLLNPKSGSGDAREVFNMHVTPVLNEAEVPYDLYVTKHSNFAIEFLSTRCLDAWCCVVAVGGDGLFHEIVNGLLQRQDWAHVLPHLALGIIPCGSGNGLARSIAHCYNEPYFSKPVLGAALTVISGRSSPMDVVRVQLQSRSLYSFLSIGWGLISDVDIESERIRMLGYQRFTVWTLYRLVNLRTYNGRISYLLTDHEVSSTHSATGYAAQRRMQSSRSCNTHIDMLNGPAPIYHSSAEYLPQEFADVISLETSINQSFRSRCDSWLSGGSRRSFYYSISESIYHSLADESEFAGLAAASLENRQQNYGPASELPDLNEPLSEDQGWLVEEGEFVMMHAVYQTHLGIDCHFAPKAQLNDGTIYLILIRAGISRPHLLSFLYNMSSGTHLPESHDDHVKVLPVRAFRLEPYDNHGIITVDGERVEFGPLQAEVLPGIARVMVPK

Target ORF information:

RefSeq Version	NM_139505.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sphingosine kinase 2 (Sk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139505.4

ORF Insert Sequence:

ATGAGCGAAT CTCTTGATAA GACCACCAGC CCGAGCTCGG CCAGTTCCAG GGCCACGCCC 
CCGGGAACGC AGGATGCGGA TGAGGGCCAC GACGTGAGCG ATACCTTCTA CACGAGCCAG 
CGCAAGAAGG GCAGCCACGT ATTTCGGGTG CGCCTTGACG CCACAGGATT CACCCTGCAG 
CGGGAGTCGC CGGGCGGTAG CATTGTTAAG GAGCAACATG TCCGCATATC GGACATTGTG 
GGTGCCCGCT GCATGCGGCC CAAGAAGAGC CGGCGCCTGG CGATGTCGGG CGCCTGTGCG 
TGCAGCTCCG GTAATCCCAA TTCGCCAGCC ATCTCGGCGT CCGGCGATCA CCATCGCCCT 
GCCACCACAC CAAGCAAATG CAGCACCAAT AGTCGGGATA ATATTCCTTC GGATGGCGGC 
GATGTCAGCG CGTTTCTCTA CGTTTTTGCC TATGTTCTGA AGAAGAGGAG CCTGCGGTCG 
GAGTTGCACC GGGAGCGAAC GGTGCTCACT CTGCGCTTCC GGTCGTTCGA CACCTTCGAG 
GACAACATGA GGGAGGCGGA TCGTTGGTAC AGATCCCTTC GCTGGCAGTT GCATCGCACG 
CTGGAGGAGA TCTTTGTGGC GCCGACGGTG GATGAGCGAC GCCGTCGAGT GCTTGTGCTG 
TTGAATCCCA AATCCGGTTC CGGTGACGCT CGTGAGGTCT TCAACATGCA CGTGACGCCG 
GTGCTCAACG AGGCCGAGGT GCCCTACGAC CTGTATGTAA CCAAGCATTC CAACTTTGCC 
ATCGAGTTCT TGAGCACCAG GTGCCTGGAC GCCTGGTGCT GCGTGGTGGC TGTCGGCGGA 
GACGGTCTCT TCCACGAGAT AGTCAATGGA CTGCTGCAGC GCCAGGACTG GGCCCACGTC 
CTGCCTCATC TGGCACTGGG AATCATTCCT TGCGGCTCCG GAAATGGATT GGCCCGCTCC 
ATTGCCCATT GTTACAACGA ACCATACTTC AGCAAGCCAG TGCTAGGAGC TGCTCTGACC 
GTAATCAGTG GACGCAGTTC ACCCATGGAC GTGGTCCGGG TGCAGCTGCA GAGTCGCTCC 
CTCTACTCCT TCCTGTCCAT CGGCTGGGGT CTGATCTCGG ACGTGGACAT CGAGAGCGAG 
CGCATTCGCA TGTTGGGCTA CCAGCGCTTC ACCGTGTGGA CCCTCTACCG TCTGGTGAAT 
CTGCGCACCT ACAACGGCCG AATCAGCTAT CTTCTGACGG ACCATGAGGT GTCCTCAACC 
CATAGCGCTA CCGGTTATGC TGCCCAGCGG AGAATGCAGA GCAGCCGTAG CTGCAACACG 
CACATCGACA TGCTAAATGG GCCGGCGCCC ATCTATCATT CCAGTGCCGA GTACCTGCCA 
CAGGAGTTTG CGGACGTGAT CTCCCTGGAG ACGTCCATCA ATCAGTCGTT CCGCTCGAGG 
TGCGACAGCT GGTTGTCGGG GGGATCGCGG CGCAGCTTTT ACTATTCCAT ATCGGAGAGC 
ATCTACCACA GTCTGGCGGA TGAGAGCGAG TTCGCCGGCC TGGCGGCCGC CTCGCTGGAA 
AACCGGCAGC AGAACTACGG TCCGGCAAGC GAGCTGCCGG ATCTGAACGA ACCGCTGTCC 
GAGGATCAGG GTTGGCTGGT GGAGGAGGGC GAGTTCGTCA TGATGCACGC CGTTTACCAG 
ACCCATCTGG GCATCGACTG TCATTTTGCG CCCAAGGCCC AGCTGAACGA CGGCACCATC 
TACCTGATCC TCATACGCGC CGGCATCAGC CGCCCGCACC TGCTGAGCTT CCTCTACAAC 
ATGAGCTCCG GCACTCACCT GCCGGAGTCG CACGACGACC ATGTGAAGGT GCTGCCAGTG 
CGAGCATTCC GCCTGGAGCC CTACGACAAT CACGGCATCA TCACGGTCGA CGGCGAGCGC 
GTCGAGTTCG GGCCCCTCCA AGCTGAGGTC CTGCCGGGCA TAGCCCGCGT CATGGTGCCC 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sk2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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