Alg10 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alg10
Entrez Gene ID	326193
Full Name	Alpha 3 glucosyltransferase
Synonyms	CG32076,CG7624,Dmel\CG32076,alg10,dAlg10
General protein information	Preferred Names Alpha 3 glucosyltransferase Names CG32076 gene product from transcript CG32076-RA Alg10-PA CG32076-PA alpha 3 glucosyltransferase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 68A9-68A9

mRNA and Protein(s)

mRNA	Protein	Name
NM_168449.2	NP_729680.1	ALG10 alpha-1,2-glucosyltransferase

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E15797g	XP_454662.1
Arabidopsis thaliana (thale cress)	AT5G02410	NP_195861.2
Pan troglodytes (chimpanzee)	ALG10	XP_003313875.1
Mus musculus (house mouse)	Alg10b	NP_001028613.1
Rattus norvegicus (Norway rat)	Alg10	NP_620801.1
Gallus gallus (chicken)	ALG10	NP_001026565.1
Schizosaccharomyces pombe (fission yeast)	alg10	NP_593221.1
Drosophila melanogaster (fruit fly)	Alg10	NP_729680.1
Xenopus tropicalis (tropical clawed frog)	alg10	NP_001016192.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002420	XP_312525.4
Danio rerio (zebrafish)	LOC100331575	XP_002661512.1
Homo sapiens (human)	ALG10	NP_116223.3
Canis lupus familiaris (dog)	ALG10	XP_534842.3
Bos taurus (cattle)	ALG10	NP_001069657.2
Saccharomyces cerevisiae (baker's yeast)	DIE2	NP_011743.2
Oryza sativa (rice)	Os04g0503700	NP_001053242.1
Eremothecium gossypii	AGOS_ADL138C	NP_983958.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Alg10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alg10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19582	NM_168449.2 Latest version!	Drosophila melanogaster ALG10 alpha-1,2-glucosyltransferase (Alg10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alg10 ( NM_168449.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729680.1 Drosophila melanogaster ALG10 alpha-1,2-glucosyltransferase (Alg10), mRNA.

Price & Availability↑

CloneID	OFa19582
Clone ID Related Accession (Same CDS sequence)	NM_168449.2
Accession Version	NM_168449.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	ALG10 alpha-1,2-glucosyltransferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168449.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATGGGT CCTGGAAACT AATCCTGCCC GTGGGCTTTG TGCTGTACTC GCTGCCACTT 
TTCCTGCGGG TGAATGGCAC ATCGGACTAT GTAATCGACG AGGAGTTCCA CATTCCGCAG 
GGATTGGCCT TTTGCCGCAA GGAATTCGAT GTGTGGGATC CCAAGATAAC AACATTTCCG 
GGCCTCTATC TGATCGCCCT GCTCCTGAAT CCCCTAAGTC TGTGCACGGT GACGGGCCTG 
AGGATGCTCA GCCTAGCAGG TGCCGGCATC AATATTCTGC TGCTCTACAA GATCAGACGG 
CGCATCCTGG CCGGCTCGGG TGGCAACTCA TATGCCGCCC ATGAGGCTAT CACCATGTCC 
GTGCTTCCGC CGCTCTACTT CTTTAGCCAC CTTTACTACA CGGACACCTT GTCGCTGACC 
ATGGTGCTTC TGTTCTACAA CTATTGGCAG CAGGAGGCGC ACCTGCCGGC GGCGGTCTTT 
GGGGCTGCCA GTGTGCTTAT GCGCCAGACG AATATCGTGT GGGTATGCAT GGCCACCGGA 
ATGACTGTGC TGGACACGTT GGTCAATCAA TGCGCCCGCA CTGGGCGCGT TCCCAAGGAA 
AATGTTCGAC TGATGGGCAA AGAGTTGTGG CTTCAACTCG TTAGCAGCCC CCAATTGCTG 
TGCAATTGCA TCCTGAGCAT CTTGGCCAAG TGCTGTTTCT ACGCTTCGAT CATTCTGCCG 
TTCGTGGGAT TCCTCTTCAT CAATGGTTCC ATTGTGGTGG GCGACAAGAG TGCCCACGAG 
GCCAGTTTGC ATGTGCCGCA GCTATTCTAC TTCGCCATCT TTGCGGCTGG CTTTGGGATA 
TCCAACACCA TACGTCAGTT TCGTCCGGCA GCAGAATTAA TACGCAGGAA TCGCGTGCTG 
TCCCTATTGG CATTGCTGCT CATACTGGTG GTGGTGCACC TAAACACCGA AGTGCATCCC 
TACCTGCTGG CTGACAACAG ACACTATACA TTCTATATAT GGAGCCGACT TTATGGACGG 
TTCTGGTGGT TCCGCTACGC CATGGCGCCC GCTTATCTGC TCTCGATTTG CGTACTCTTT 
TGCGGACTAC GTCACATGCC GGAAAGCTTT AAGTTAATGT TTCCACTTAG TCTATTCCTG 
GTGCTGTGCT TCCAGCGACT CCTGGAGCTG CGCTACTTCC TGGTGCCGTA CATTCTGTTT 
CGACTCAACA CACGGCACAC TCGCAAGGGT TACGCCGAGT GGCTGGAGCT CGGAGCTCAT 
CTGCTGCTCA ATGTGGCCAC GTTTTACGTG TACTTTACAA AGGAGTTCTA CTGGAAAAAC 
TACCGCACTC CTCAAAGGAT CATATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729680.1
CDS	106..1455
Translation	MNGSWKLILPVGFVLYSLPLFLRVNGTSDYVIDEEFHIPQGLAFCRKEFDVWDPKITTFPGLYLIALLLNPLSLCTVTGLRMLSLAGAGINILLLYKIRRRILAGSGGNSYAAHEAITMSVLPPLYFFSHLYYTDTLSLTMVLLFYNYWQQEAHLPAAVFGAASVLMRQTNIVWVCMATGMTVLDTLVNQCARTGRVPKENVRLMGKELWLQLVSSPQLLCNCILSILAKCCFYASIILPFVGFLFINGSIVVGDKSAHEASLHVPQLFYFAIFAAGFGISNTIRQFRPAAELIRRNRVLSLLALLLILVVVHLNTEVHPYLLADNRHYTFYIWSRLYGRFWWFRYAMAPAYLLSICVLFCGLRHMPESFKLMFPLSLFLVLCFQRLLELRYFLVPYILFRLNTRHTRKGYAEWLELGAHLLLNVATFYVYFTKEFYWKNYRTPQRIIW

Target ORF information:

RefSeq Version	NM_168449.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ALG10 alpha-1,2-glucosyltransferase (Alg10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168449.2

ORF Insert Sequence:

ATGAATGGGT CCTGGAAACT AATCCTGCCC GTGGGCTTTG TGCTGTACTC GCTGCCACTT 
TTCCTGCGGG TGAATGGCAC ATCGGACTAT GTAATCGACG AGGAGTTCCA CATTCCGCAG 
GGATTGGCCT TTTGCCGCAA GGAATTCGAT GTGTGGGATC CCAAGATAAC AACATTTCCG 
GGCCTCTATC TGATCGCCCT GCTCCTGAAT CCCCTAAGTC TGTGCACGGT GACGGGCCTG 
AGGATGCTCA GCCTAGCAGG TGCCGGCATC AATATTCTGC TGCTCTACAA GATCAGACGG 
CGCATCCTGG CCGGCTCGGG TGGCAACTCA TATGCCGCCC ATGAGGCTAT CACCATGTCC 
GTGCTTCCGC CGCTCTACTT CTTTAGCCAC CTTTACTACA CGGACACCTT GTCGCTGACC 
ATGGTGCTTC TGTTCTACAA CTATTGGCAG CAGGAGGCGC ACCTGCCGGC GGCGGTCTTT 
GGGGCTGCCA GTGTGCTTAT GCGCCAGACG AATATCGTGT GGGTATGCAT GGCCACCGGA 
ATGACTGTGC TGGACACGTT GGTCAATCAA TGCGCCCGCA CTGGGCGCGT TCCCAAGGAA 
AATGTTCGAC TGATGGGCAA AGAGTTGTGG CTTCAACTCG TTAGCAGCCC CCAATTGCTG 
TGCAATTGCA TCCTGAGCAT CTTGGCCAAG TGCTGTTTCT ACGCTTCGAT CATTCTGCCG 
TTCGTGGGAT TCCTCTTCAT CAATGGTTCC ATTGTGGTGG GCGACAAGAG TGCCCACGAG 
GCCAGTTTGC ATGTGCCGCA GCTATTCTAC TTCGCCATCT TTGCGGCTGG CTTTGGGATA 
TCCAACACCA TACGTCAGTT TCGTCCGGCA GCAGAATTAA TACGCAGGAA TCGCGTGCTG 
TCCCTATTGG CATTGCTGCT CATACTGGTG GTGGTGCACC TAAACACCGA AGTGCATCCC 
TACCTGCTGG CTGACAACAG ACACTATACA TTCTATATAT GGAGCCGACT TTATGGACGG 
TTCTGGTGGT TCCGCTACGC CATGGCGCCC GCTTATCTGC TCTCGATTTG CGTACTCTTT 
TGCGGACTAC GTCACATGCC GGAAAGCTTT AAGTTAATGT TTCCACTTAG TCTATTCCTG 
GTGCTGTGCT TCCAGCGACT CCTGGAGCTG CGCTACTTCC TGGTGCCGTA CATTCTGTTT 
CGACTCAACA CACGGCACAC TCGCAAGGGT TACGCCGAGT GGCTGGAGCT CGGAGCTCAT 
CTGCTGCTCA ATGTGGCCAC GTTTTACGTG TACTTTACAA AGGAGTTCTA CTGGAAAAAC 
TACCGCACTC CTCAAAGGAT CATATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Alg10 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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