Atg16 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atg16
Entrez Gene ID	326115
Full Name	Autophagy-related 16
Synonyms	CG15513,CG18040,CG18683,CG31033,Dmel\CG31033,atg16
General protein information	Preferred Names Autophagy-related 16 Names CG31033 gene product from transcript CG31033-RC Atg16-PA Atg16-PB Atg16-PC Atg16-PF Autophagy-specific gene 16 CG31033-PA CG31033-PB CG31033-PC CG31033-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99C2-99C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144652.2	NP_001138124.2	Autophagy-related 16, isoform F
NM_170433.3	NP_733312.2	Autophagy-related 16, isoform B
NM_170434.4	NP_733313.2	Autophagy-related 16, isoform C
NM_170432.3	NP_733311.2	Autophagy-related 16, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATG16L1	XP_850664.1
Drosophila melanogaster (fruit fly)	CG31033	NP_733313.2
Bos taurus (cattle)	ATG16L1	NP_001178318.1
Rattus norvegicus (Norway rat)	Atg16l1	NP_001102279.2
Xenopus tropicalis (tropical clawed frog)	atg16l1	NP_001017343.1
Oryza sativa (rice)	Os03g0746600	NP_001051251.1
Pan troglodytes (chimpanzee)	ATG16L1	XP_001150112.1
Gallus gallus (chicken)	ATG16L1	XP_004936997.1
Arabidopsis thaliana (thale cress)	AT5G50230	NP_199834.2
Homo sapiens (human)	ATG16L1	NP_110430.5
Macaca mulatta (Rhesus monkey)	ATG16L1	XP_001114814.1
Mus musculus (house mouse)	Atg16l1	NP_084122.2
Danio rerio (zebrafish)	atg16l1	NP_001017854.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Atg16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atg16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa07202	NM_170432.3 Latest version!	Drosophila melanogaster Autophagy-related 16 (Atg16), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa07203	NM_170433.3 Latest version!	Drosophila melanogaster Autophagy-related 16 (Atg16), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa07201	NM_170434.4 Latest version!	Drosophila melanogaster Autophagy-related 16 (Atg16), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa22338	NM_001144652.2 Latest version!	Drosophila melanogaster Autophagy-related 16 (Atg16), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atg16 ( NM_170432.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733311.2 Drosophila melanogaster Autophagy-related 16 (Atg16), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa07202
Clone ID Related Accession (Same CDS sequence)	NM_170432.3
Accession Version	NM_170432.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 16, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 26, 2009 this sequence version replaced NM_170432.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAATACA AGTCAAAGGA TGCCGACAAG TTGAACGAGG AAAACGAGAG CATAATTAGG 
AAACGTTCGG CTAAACTGAA ACGTGACCTA GAAGACGCGG TTCGTGAGCC CAGTTCGTCC 
AGCAACGCCG CCTCCTCGCC CGGCGCCGCA TCCTTGCAGC GCAACTCCAG TCCCGCCCAG 
TTCGTTGGCG GCCTCATCGG CGACGAGGAT TTCGACGAGG CCGCGATAAA TGGCGCCATG 
GAGGCCATAG GTCTCGATGA TAATGAATAT ATTAGCGCTC GTTTTACAGC AGGCGAAATC 
GCCGAGAACT CGCGGGCTTC CATCGACACC CTGAAGGCCA CGGGCTACCT GGGCCAGGCC 
AATCCCACCA AGATCCTCAT GAAGTTCGAG GCCCACGAGA ACGAGTCGCA CGCGGTGCGC 
TGGAGTCCCG TGGAGCGCAT GGTGGCCACC GGTGGCGCTG ATCGCAAAGT CAAACTTTGG 
GACATTGGCA AAAATTCCAC CGAACCACGT GCCGTTCTAA GCGGGAGCAG TGCTGGGATT 
AACTCCGTAG ATTTCGATTC GACAGGCGCC TACATCCTGG GCACCTCCAA TGACTACGGA 
GCCAGAGTAT GGACAGTGAT GGACAATCGC TTAAGACACA CGCTGACGGG TCACAGCGGC 
AAGGTAATGG CCGCCAAGTA CGTCCAGGAG CCCATCAAAG TGGTGACCGG TAGCCACGAT 
CGCACACTGA AGATCTGGGA TTTGCGCAGC ATCGCCTGCA TAGAGACGAA GTTCGCCGGC 
TCCAGCTGCA ATGATCTAGT CACCACCGAC AGCCTCGGAT CAACGATCAT CAGTGGGCAC 
TACGATAAGA AGATCCGGTT CTGGGACATA CGCACCGAGA AGCAGGCGGA CGACGTCTTA 
ATGCCGGCCA AGATCACCTC TTTGGATTTG TCAAAAGATT GCAATTACCT CATATGCTCT 
GTAAGAGACG ATACTATTAA ACTGTTAGAT TTACGCAAGA ACCAAGTTAT ATCCACGTTT 
ACAAACGAAC ACTTTAAGAT CAGCTGTGAT TTTGCTCGAG CTTCTTTCAA CTCGAGCGGC 
CTAAAGATCG CTTGTGGATC AGCGGATGGC GCTATATACA TTTGGAATGT GAATGGGTTC 
CTGGAAGCCA CACTCAAGGG ACACAGCACC GCCGTAAACG CGGTCAGTTG GAGTCCCAAC 
AACAACATGC TGGCCTCCGT GGGAAAGAAC AAGCGCTGCA CCATCTACTC GGAGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733311.2
CDS	121..1380
Translation	MQYKSKDADKLNEENESIIRKRSAKLKRDLEDAVREPSSSSNAASSPGAASLQRNSSPAQFVGGLIGDEDFDEAAINGAMEAIGLDDNEYISARFTAGEIAENSRASIDTLKATGYLGQANPTKILMKFEAHENESHAVRWSPVERMVATGGADRKVKLWDIGKNSTEPRAVLSGSSAGINSVDFDSTGAYILGTSNDYGARVWTVMDNRLRHTLTGHSGKVMAAKYVQEPIKVVTGSHDRTLKIWDLRSIACIETKFAGSSCNDLVTTDSLGSTIISGHYDKKIRFWDIRTEKQADDVLMPAKITSLDLSKDCNYLICSVRDDTIKLLDLRKNQVISTFTNEHFKISCDFARASFNSSGLKIACGSADGAIYIWNVNGFLEATLKGHSTAVNAVSWSPNNNMLASVGKNKRCTIYSES

Target ORF information:

RefSeq Version	NM_170432.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 16 (Atg16), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170432.3

ORF Insert Sequence:

ATGCAATACA AGTCAAAGGA TGCCGACAAG TTGAACGAGG AAAACGAGAG CATAATTAGG 
AAACGTTCGG CTAAACTGAA ACGTGACCTA GAAGACGCGG TTCGTGAGCC CAGTTCGTCC 
AGCAACGCCG CCTCCTCGCC CGGCGCCGCA TCCTTGCAGC GCAACTCCAG TCCCGCCCAG 
TTCGTTGGCG GCCTCATCGG CGACGAGGAT TTCGACGAGG CCGCGATAAA TGGCGCCATG 
GAGGCCATAG GTCTCGATGA TAATGAATAT ATTAGCGCTC GTTTTACAGC AGGCGAAATC 
GCCGAGAACT CGCGGGCTTC CATCGACACC CTGAAGGCCA CGGGCTACCT GGGCCAGGCC 
AATCCCACCA AGATCCTCAT GAAGTTCGAG GCCCACGAGA ACGAGTCGCA CGCGGTGCGC 
TGGAGTCCCG TGGAGCGCAT GGTGGCCACC GGTGGCGCTG ATCGCAAAGT CAAACTTTGG 
GACATTGGCA AAAATTCCAC CGAACCACGT GCCGTTCTAA GCGGGAGCAG TGCTGGGATT 
AACTCCGTAG ATTTCGATTC GACAGGCGCC TACATCCTGG GCACCTCCAA TGACTACGGA 
GCCAGAGTAT GGACAGTGAT GGACAATCGC TTAAGACACA CGCTGACGGG TCACAGCGGC 
AAGGTAATGG CCGCCAAGTA CGTCCAGGAG CCCATCAAAG TGGTGACCGG TAGCCACGAT 
CGCACACTGA AGATCTGGGA TTTGCGCAGC ATCGCCTGCA TAGAGACGAA GTTCGCCGGC 
TCCAGCTGCA ATGATCTAGT CACCACCGAC AGCCTCGGAT CAACGATCAT CAGTGGGCAC 
TACGATAAGA AGATCCGGTT CTGGGACATA CGCACCGAGA AGCAGGCGGA CGACGTCTTA 
ATGCCGGCCA AGATCACCTC TTTGGATTTG TCAAAAGATT GCAATTACCT CATATGCTCT 
GTAAGAGACG ATACTATTAA ACTGTTAGAT TTACGCAAGA ACCAAGTTAT ATCCACGTTT 
ACAAACGAAC ACTTTAAGAT CAGCTGTGAT TTTGCTCGAG CTTCTTTCAA CTCGAGCGGC 
CTAAAGATCG CTTGTGGATC AGCGGATGGC GCTATATACA TTTGGAATGT GAATGGGTTC 
CTGGAAGCCA CACTCAAGGG ACACAGCACC GCCGTAAACG CGGTCAGTTG GAGTCCCAAC 
AACAACATGC TGGCCTCCGT GGGAAAGAAC AAGCGCTGCA CCATCTACTC GGAGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg16 ( NM_170433.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733312.2 Drosophila melanogaster Autophagy-related 16 (Atg16), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa07203
Clone ID Related Accession (Same CDS sequence)	NM_170433.3
Accession Version	NM_170433.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 16, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 26, 2009 this sequence version replaced NM_170433.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAAGC GTCTATTAAA AAGCTTTTCG TTCTCTTACA ATTCACAGGA TGAAAATCGG 
CGTCTACTGG AGCGACTTAT GCAATACAAG TCAAAGGATG CCGACAAGTT GAACGAGGAA 
AACGAGAGCA TAATTAGGAA ACGTTCGGCT AAACTGAAAC GTGACCTAGA AGACGCGGTT 
CGTGAGCCCA GTTCGTCCAG CAACGCCGCC TCCTCGCCCG GCGCCGCATC CTTGCAGCGC 
AACTCCAGTC CCGCCCAGTT CGTTGGCGGC CTCATCGGCG ACGAGGATTT CGACGAGGCC 
GCGATAAATG GCGCCATGGA GGCCATAGGT CTCGATGATA ATGAATATAT TAGCGCTCGT 
TTTACAGCAG GCGAAATCGC CGAGAACTCG CGGGCTTCCA TCGACACCCT GAAGGCCACG 
GGCTACCTGG GCCAGGCCAA TCCCACCAAG ATCCTCATGA AGTTCGAGGC CCACGAGAAC 
GAGTCGCACG CGGTGCGCTG GAGTCCCGTG GAGCGCATGG TGGCCACCGG TGGCGCTGAT 
CGCAAAGTCA AACTTTGGGA CATTGGCAAA AATTCCACCG AACCACGTGC CGTTCTAAGC 
GGGAGCAGTG CTGGGATTAA CTCCGTAGAT TTCGATTCGA CAGGCGCCTA CATCCTGGGC 
ACCTCCAATG ACTACGGAGC CAGAGTATGG ACAGTGATGG ACAATCGCTT AAGACACACG 
CTGACGGGTC ACAGCGGCAA GGTAATGGCC GCCAAGTACG TCCAGGAGCC CATCAAAGTG 
GTGACCGGTA GCCACGATCG CACACTGAAG ATCTGGGATT TGCGCAGCAT CGCCTGCATA 
GAGACGAAGT TCGCCGGCTC CAGCTGCAAT GATCTAGTCA CCACCGACAG CCTCGGATCA 
ACGATCATCA GTGGGCACTA CGATAAGAAG ATCCGGTTCT GGGACATACG CACCGAGAAG 
CAGGCGGACG ACGTCTTAAT GCCGGCCAAG ATCACCTCTT TGGATTTGTC AAAAGATTGC 
AATTACCTCA TATGCTCTGT AAGAGACGAT ACTATTAAAC TGTTAGATTT ACGCAAGAAC 
CAAGTTATAT CCACGTTTAC AAACGAACAC TTTAAGATCA GCTGTGATTT TGCTCGAGCT 
TCTTTCAACT CGAGCGGCCT AAAGATCGCT TGTGGATCAG CGGATGGCGC TATATACATT 
TGGAATGTGA ATGGGTTCCT GGAAGCCACA CTCAAGGGAC ACAGCACCGC CGTAAACGCG 
GTCAGTTGGA GTCCCAACAA CAACATGCTG GCCTCCGTGG GAAAGAACAA GCGCTGCACC 
ATCTACTCGG AGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733312.2
CDS	52..1389
Translation	MKKRLLKSFSFSYNSQDENRRLLERLMQYKSKDADKLNEENESIIRKRSAKLKRDLEDAVREPSSSSNAASSPGAASLQRNSSPAQFVGGLIGDEDFDEAAINGAMEAIGLDDNEYISARFTAGEIAENSRASIDTLKATGYLGQANPTKILMKFEAHENESHAVRWSPVERMVATGGADRKVKLWDIGKNSTEPRAVLSGSSAGINSVDFDSTGAYILGTSNDYGARVWTVMDNRLRHTLTGHSGKVMAAKYVQEPIKVVTGSHDRTLKIWDLRSIACIETKFAGSSCNDLVTTDSLGSTIISGHYDKKIRFWDIRTEKQADDVLMPAKITSLDLSKDCNYLICSVRDDTIKLLDLRKNQVISTFTNEHFKISCDFARASFNSSGLKIACGSADGAIYIWNVNGFLEATLKGHSTAVNAVSWSPNNNMLASVGKNKRCTIYSES

Target ORF information:

RefSeq Version	NM_170433.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 16 (Atg16), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170433.3

ORF Insert Sequence:

ATGAAAAAGC GTCTATTAAA AAGCTTTTCG TTCTCTTACA ATTCACAGGA TGAAAATCGG 
CGTCTACTGG AGCGACTTAT GCAATACAAG TCAAAGGATG CCGACAAGTT GAACGAGGAA 
AACGAGAGCA TAATTAGGAA ACGTTCGGCT AAACTGAAAC GTGACCTAGA AGACGCGGTT 
CGTGAGCCCA GTTCGTCCAG CAACGCCGCC TCCTCGCCCG GCGCCGCATC CTTGCAGCGC 
AACTCCAGTC CCGCCCAGTT CGTTGGCGGC CTCATCGGCG ACGAGGATTT CGACGAGGCC 
GCGATAAATG GCGCCATGGA GGCCATAGGT CTCGATGATA ATGAATATAT TAGCGCTCGT 
TTTACAGCAG GCGAAATCGC CGAGAACTCG CGGGCTTCCA TCGACACCCT GAAGGCCACG 
GGCTACCTGG GCCAGGCCAA TCCCACCAAG ATCCTCATGA AGTTCGAGGC CCACGAGAAC 
GAGTCGCACG CGGTGCGCTG GAGTCCCGTG GAGCGCATGG TGGCCACCGG TGGCGCTGAT 
CGCAAAGTCA AACTTTGGGA CATTGGCAAA AATTCCACCG AACCACGTGC CGTTCTAAGC 
GGGAGCAGTG CTGGGATTAA CTCCGTAGAT TTCGATTCGA CAGGCGCCTA CATCCTGGGC 
ACCTCCAATG ACTACGGAGC CAGAGTATGG ACAGTGATGG ACAATCGCTT AAGACACACG 
CTGACGGGTC ACAGCGGCAA GGTAATGGCC GCCAAGTACG TCCAGGAGCC CATCAAAGTG 
GTGACCGGTA GCCACGATCG CACACTGAAG ATCTGGGATT TGCGCAGCAT CGCCTGCATA 
GAGACGAAGT TCGCCGGCTC CAGCTGCAAT GATCTAGTCA CCACCGACAG CCTCGGATCA 
ACGATCATCA GTGGGCACTA CGATAAGAAG ATCCGGTTCT GGGACATACG CACCGAGAAG 
CAGGCGGACG ACGTCTTAAT GCCGGCCAAG ATCACCTCTT TGGATTTGTC AAAAGATTGC 
AATTACCTCA TATGCTCTGT AAGAGACGAT ACTATTAAAC TGTTAGATTT ACGCAAGAAC 
CAAGTTATAT CCACGTTTAC AAACGAACAC TTTAAGATCA GCTGTGATTT TGCTCGAGCT 
TCTTTCAACT CGAGCGGCCT AAAGATCGCT TGTGGATCAG CGGATGGCGC TATATACATT 
TGGAATGTGA ATGGGTTCCT GGAAGCCACA CTCAAGGGAC ACAGCACCGC CGTAAACGCG 
GTCAGTTGGA GTCCCAACAA CAACATGCTG GCCTCCGTGG GAAAGAACAA GCGCTGCACC 
ATCTACTCGG AGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg16 ( NM_170434.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733313.2 Drosophila melanogaster Autophagy-related 16 (Atg16), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa07201
Clone ID Related Accession (Same CDS sequence)	NM_170434.4
Accession Version	NM_170434.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 16, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 26, 2009 this sequence version replaced NM_170434.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTACGG AGGAGCATGT GTGGCGAGCG CACGTCGTGC GGCGCTTACG CGAGCGAAAT 
CGCAAGGAGT GCGACAACTT CAAGGAGATC ATCGAGCAAA ACAACCGACT GATTGACCAT 
GTGGCGCAAC TGAAGGCGGA CAACCTGAAG ATCTCCGTCG AAAACGAGCA GCTCCGCAAC 
GCGGTGTCAA CAGGTGGCAC CGGCTCCAAC GTGGCTATCG CCACCCTCGA AAAGAAGCTG 
CTCAGCCAGC AGGAGGAGCT CACAGAGCTA CACAAGCGCA AGGGCGAGAA CTCCCAGATG 
ATCGTGGACC TCAACCAGAA GGTCGAGCAG CAGAGGATCA TAATTTCCGA GAAGGAGCAC 
AGCCTCGTCG AACAGCAAAC GAACAACAAC CGATTGCGCG CGGAGGTGCA GCTACTTCAC 
TCGAGTTTGG AGGAACTCAA GAAGCTGAAC AACACAATGC TGGACGAACA TACGGCGCTT 
CAATTGGCAT TTAGCTCCCT CGAGGAGAAG TTAAGGGGAG TGCAAGATGA AAATCGGCGT 
CTACTGGAGC GACTTATGCA ATACAAGTCA AAGGATGCCG ACAAGTTGAA CGAGGAAAAC 
GAGAGCATAA TTAGGAAACG TTCGGCTAAA CTGAAACGTG ACCTAGAAGA CGCGGTTCGT 
GAGCCCAGTT CGTCCAGCAA CGCCGCCTCC TCGCCCGGCG CCGCATCCTT GCAGCGCAAC 
TCCAGTCCCG CCCAGTTCGT TGGCGGCCTC ATCGGCGACG AGGATTTCGA CGAGGCCGCG 
ATAAATGGCG CCATGGAGGC CATAGGTCTC GATGATAATG AATATATTAG CGCTCGTTTT 
ACAGCAGGCG AAATCGCCGA GAACTCGCGG GCTTCCATCG ACACCCTGAA GGCCACGGGC 
TACCTGGGCC AGGCCAATCC CACCAAGATC CTCATGAAGT TCGAGGCCCA CGAGAACGAG 
TCGCACGCGG TGCGCTGGAG TCCCGTGGAG CGCATGGTGG CCACCGGTGG CGCTGATCGC 
AAAGTCAAAC TTTGGGACAT TGGCAAAAAT TCCACCGAAC CACGTGCCGT TCTAAGCGGG 
AGCAGTGCTG GGATTAACTC CGTAGATTTC GATTCGACAG GCGCCTACAT CCTGGGCACC 
TCCAATGACT ACGGAGCCAG AGTATGGACA GTGATGGACA ATCGCTTAAG ACACACGCTG 
ACGGGTCACA GCGGCAAGGT AATGGCCGCC AAGTACGTCC AGGAGCCCAT CAAAGTGGTG 
ACCGGTAGCC ACGATCGCAC ACTGAAGATC TGGGATTTGC GCAGCATCGC CTGCATAGAG 
ACGAAGTTCG CCGGCTCCAG CTGCAATGAT CTAGTCACCA CCGACAGCCT CGGATCAACG 
ATCATCAGTG GGCACTACGA TAAGAAGATC CGGTTCTGGG ACATACGCAC CGAGAAGCAG 
GCGGACGACG TCTTAATGCC GGCCAAGATC ACCTCTTTGG ATTTGTCAAA AGATTGCAAT 
TACCTCATAT GCTCTGTAAG AGACGATACT ATTAAACTGT TAGATTTACG CAAGAACCAA 
GTTATATCCA CGTTTACAAA CGAACACTTT AAGATCAGCT GTGATTTTGC TCGAGCTTCT 
TTCAACTCGA GCGGCCTAAA GATCGCTTGT GGATCAGCGG ATGGCGCTAT ATACATTTGG 
AATGTGAATG GGTTCCTGGA AGCCACACTC AAGGGACACA GCACCGCCGT AAACGCGGTC 
AGTTGGAGTC CCAACAACAA CATGCTGGCC TCCGTGGGAA AGAACAAGCG CTGCACCATC 
TACTCGGAGT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733313.2
CDS	257..2071
Translation	MSTEEHVWRAHVVRRLRERNRKECDNFKEIIEQNNRLIDHVAQLKADNLKISVENEQLRNAVSTGGTGSNVAIATLEKKLLSQQEELTELHKRKGENSQMIVDLNQKVEQQRIIISEKEHSLVEQQTNNNRLRAEVQLLHSSLEELKKLNNTMLDEHTALQLAFSSLEEKLRGVQDENRRLLERLMQYKSKDADKLNEENESIIRKRSAKLKRDLEDAVREPSSSSNAASSPGAASLQRNSSPAQFVGGLIGDEDFDEAAINGAMEAIGLDDNEYISARFTAGEIAENSRASIDTLKATGYLGQANPTKILMKFEAHENESHAVRWSPVERMVATGGADRKVKLWDIGKNSTEPRAVLSGSSAGINSVDFDSTGAYILGTSNDYGARVWTVMDNRLRHTLTGHSGKVMAAKYVQEPIKVVTGSHDRTLKIWDLRSIACIETKFAGSSCNDLVTTDSLGSTIISGHYDKKIRFWDIRTEKQADDVLMPAKITSLDLSKDCNYLICSVRDDTIKLLDLRKNQVISTFTNEHFKISCDFARASFNSSGLKIACGSADGAIYIWNVNGFLEATLKGHSTAVNAVSWSPNNNMLASVGKNKRCTIYSES

Target ORF information:

RefSeq Version	NM_170434.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 16 (Atg16), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170434.4

ORF Insert Sequence:

ATGTCTACGG AGGAGCATGT GTGGCGAGCG CACGTCGTGC GGCGCTTACG CGAGCGAAAT 
CGCAAGGAGT GCGACAACTT CAAGGAGATC ATCGAGCAAA ACAACCGACT GATTGACCAT 
GTGGCGCAAC TGAAGGCGGA CAACCTGAAG ATCTCCGTCG AAAACGAGCA GCTCCGCAAC 
GCGGTGTCAA CAGGTGGCAC CGGCTCCAAC GTGGCTATCG CCACCCTCGA AAAGAAGCTG 
CTCAGCCAGC AGGAGGAGCT CACAGAGCTA CACAAGCGCA AGGGCGAGAA CTCCCAGATG 
ATCGTGGACC TCAACCAGAA GGTCGAGCAG CAGAGGATCA TAATTTCCGA GAAGGAGCAC 
AGCCTCGTCG AACAGCAAAC GAACAACAAC CGATTGCGCG CGGAGGTGCA GCTACTTCAC 
TCGAGTTTGG AGGAACTCAA GAAGCTGAAC AACACAATGC TGGACGAACA TACGGCGCTT 
CAATTGGCAT TTAGCTCCCT CGAGGAGAAG TTAAGGGGAG TGCAAGATGA AAATCGGCGT 
CTACTGGAGC GACTTATGCA ATACAAGTCA AAGGATGCCG ACAAGTTGAA CGAGGAAAAC 
GAGAGCATAA TTAGGAAACG TTCGGCTAAA CTGAAACGTG ACCTAGAAGA CGCGGTTCGT 
GAGCCCAGTT CGTCCAGCAA CGCCGCCTCC TCGCCCGGCG CCGCATCCTT GCAGCGCAAC 
TCCAGTCCCG CCCAGTTCGT TGGCGGCCTC ATCGGCGACG AGGATTTCGA CGAGGCCGCG 
ATAAATGGCG CCATGGAGGC CATAGGTCTC GATGATAATG AATATATTAG CGCTCGTTTT 
ACAGCAGGCG AAATCGCCGA GAACTCGCGG GCTTCCATCG ACACCCTGAA GGCCACGGGC 
TACCTGGGCC AGGCCAATCC CACCAAGATC CTCATGAAGT TCGAGGCCCA CGAGAACGAG 
TCGCACGCGG TGCGCTGGAG TCCCGTGGAG CGCATGGTGG CCACCGGTGG CGCTGATCGC 
AAAGTCAAAC TTTGGGACAT TGGCAAAAAT TCCACCGAAC CACGTGCCGT TCTAAGCGGG 
AGCAGTGCTG GGATTAACTC CGTAGATTTC GATTCGACAG GCGCCTACAT CCTGGGCACC 
TCCAATGACT ACGGAGCCAG AGTATGGACA GTGATGGACA ATCGCTTAAG ACACACGCTG 
ACGGGTCACA GCGGCAAGGT AATGGCCGCC AAGTACGTCC AGGAGCCCAT CAAAGTGGTG 
ACCGGTAGCC ACGATCGCAC ACTGAAGATC TGGGATTTGC GCAGCATCGC CTGCATAGAG 
ACGAAGTTCG CCGGCTCCAG CTGCAATGAT CTAGTCACCA CCGACAGCCT CGGATCAACG 
ATCATCAGTG GGCACTACGA TAAGAAGATC CGGTTCTGGG ACATACGCAC CGAGAAGCAG 
GCGGACGACG TCTTAATGCC GGCCAAGATC ACCTCTTTGG ATTTGTCAAA AGATTGCAAT 
TACCTCATAT GCTCTGTAAG AGACGATACT ATTAAACTGT TAGATTTACG CAAGAACCAA 
GTTATATCCA CGTTTACAAA CGAACACTTT AAGATCAGCT GTGATTTTGC TCGAGCTTCT 
TTCAACTCGA GCGGCCTAAA GATCGCTTGT GGATCAGCGG ATGGCGCTAT ATACATTTGG 
AATGTGAATG GGTTCCTGGA AGCCACACTC AAGGGACACA GCACCGCCGT AAACGCGGTC 
AGTTGGAGTC CCAACAACAA CATGCTGGCC TCCGTGGGAA AGAACAAGCG CTGCACCATC 
TACTCGGAGT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg16 ( NM_001144652.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138124.2 Drosophila melanogaster Autophagy-related 16 (Atg16), transcript variant F, mRNA.

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CloneID	OFa22338
Clone ID Related Accession (Same CDS sequence)	NM_001144652.2
Accession Version	NM_001144652.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 16, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001144652.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTACGG AGGAGCATGT GTGGCGAGCG CACGTCGTGC GGCGCTTACG CGAGCGAAAT 
CGCAAGGAGT GCGACAACTT CAAGGAGATC ATCGAGCAAA ACAACCGACT GATTGACCAT 
GTGGCGCAAC TGAAGGCGGA CAACCTGAAG ATCTCCGTCG AAAACGAGCA GCTCCGCAAC 
GCGGTGTCAA CAGGTGGCAC CGGCTCCAAC GTGGCTATCG CCACCCTCGA AAAGAAGCTG 
CTCAGCCAGC AGGAGGAGCT CACAGAGCTA CACAAGCGCA AGGGCGAGAA CTCCCAGATG 
ATCGTGGACC TCAACCAGAA GGTCGAGCAG CAGAGGATCA TAATTTCCGA GAAGGAGCAC 
AGCCTCGTCG AACAGCAAAC GAACAACAAC CGATTGCGCG CGGAGGTGCA GCTACTTCAC 
TCGAGTTTGG AGGAACTCAA GAAGCTGAAC AACACAATGC TGGACGAACA TACGGCGCTT 
CAATTGGCAT TTAGCTCCCT CGAGGAGAAG TTAAGGGGAG TGCAAGATGA AAATCGGCGT 
CTACTGGAGC GACTTATGCA ATACAAGTCA AAGGATGCCG ACAAGTTGAA CGAGGAAAAC 
GAGAGCATAA TTAGAAAAAG ACTGCCGTCA ATTTTCAGGA AACGTTCGGC TAAACTGAAA 
CGTGACCTAG AAGACGCGGT TCGTGAGCCC AGTTCGTCCA GCAACGCCGC CTCCTCGCCC 
GGCGCCGCAT CCTTGCAGCG CAACTCCAGT CCCGCCCAGT TCGTTGGCGG CCTCATCGGC 
GACGAGGATT TCGACGAGGC CGCGATAAAT GGCGCCATGG AGGCCATAGG TCTCGATGAT 
AATGAATATA TTAGCGCTCG TTTTACAGCA GGCGAAATCG CCGAGAACTC GCGGGCTTCC 
ATCGACACCC TGAAGGCCAC GGGCTACCTG GGCCAGGCCA ATCCCACCAA GATCCTCATG 
AAGTTCGAGG CCCACGAGAA CGAGTCGCAC GCGGTGCGCT GGAGTCCCGT GGAGCGCATG 
GTGGCCACCG GTGGCGCTGA TCGCAAAGTC AAACTTTGGG ACATTGGCAA AAATTCCACC 
GAACCACGTG CCGTTCTAAG CGGGAGCAGT GCTGGGATTA ACTCCGTAGA TTTCGATTCG 
ACAGGCGCCT ACATCCTGGG CACCTCCAAT GACTACGGAG CCAGAGTATG GACAGTGATG 
GACAATCGCT TAAGACACAC GCTGACGGGT CACAGCGGCA AGGTAATGGC CGCCAAGTAC 
GTCCAGGAGC CCATCAAAGT GGTGACCGGT AGCCACGATC GCACACTGAA GATCTGGGAT 
TTGCGCAGCA TCGCCTGCAT AGAGACGAAG TTCGCCGGCT CCAGCTGCAA TGATCTAGTC 
ACCACCGACA GCCTCGGATC AACGATCATC AGTGGGCACT ACGATAAGAA GATCCGGTTC 
TGGGACATAC GCACCGAGAA GCAGGCGGAC GACGTCTTAA TGCCGGCCAA GATCACCTCT 
TTGGATTTGT CAAAAGATTG CAATTACCTC ATATGCTCTG TAAGAGACGA TACTATTAAA 
CTGTTAGATT TACGCAAGAA CCAAGTTATA TCCACGTTTA CAAACGAACA CTTTAAGATC 
AGCTGTGATT TTGCTCGAGC TTCTTTCAAC TCGAGCGGCC TAAAGATCGC TTGTGGATCA 
GCGGATGGCG CTATATACAT TTGGAATGTG AATGGGTTCC TGGAAGCCAC ACTCAAGGGA 
CACAGCACCG CCGTAAACGC GGTCAGTTGG AGTCCCAACA ACAACATGCT GGCCTCCGTG 
GGAAAGAACA AGCGCTGCAC CATCTACTCG GAGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138124.2
CDS	257..2095
Translation	MSTEEHVWRAHVVRRLRERNRKECDNFKEIIEQNNRLIDHVAQLKADNLKISVENEQLRNAVSTGGTGSNVAIATLEKKLLSQQEELTELHKRKGENSQMIVDLNQKVEQQRIIISEKEHSLVEQQTNNNRLRAEVQLLHSSLEELKKLNNTMLDEHTALQLAFSSLEEKLRGVQDENRRLLERLMQYKSKDADKLNEENESIIRKRLPSIFRKRSAKLKRDLEDAVREPSSSSNAASSPGAASLQRNSSPAQFVGGLIGDEDFDEAAINGAMEAIGLDDNEYISARFTAGEIAENSRASIDTLKATGYLGQANPTKILMKFEAHENESHAVRWSPVERMVATGGADRKVKLWDIGKNSTEPRAVLSGSSAGINSVDFDSTGAYILGTSNDYGARVWTVMDNRLRHTLTGHSGKVMAAKYVQEPIKVVTGSHDRTLKIWDLRSIACIETKFAGSSCNDLVTTDSLGSTIISGHYDKKIRFWDIRTEKQADDVLMPAKITSLDLSKDCNYLICSVRDDTIKLLDLRKNQVISTFTNEHFKISCDFARASFNSSGLKIACGSADGAIYIWNVNGFLEATLKGHSTAVNAVSWSPNNNMLASVGKNKRCTIYSES

Target ORF information:

RefSeq Version	NM_001144652.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 16 (Atg16), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144652.2

ORF Insert Sequence:

ATGTCTACGG AGGAGCATGT GTGGCGAGCG CACGTCGTGC GGCGCTTACG CGAGCGAAAT 
CGCAAGGAGT GCGACAACTT CAAGGAGATC ATCGAGCAAA ACAACCGACT GATTGACCAT 
GTGGCGCAAC TGAAGGCGGA CAACCTGAAG ATCTCCGTCG AAAACGAGCA GCTCCGCAAC 
GCGGTGTCAA CAGGTGGCAC CGGCTCCAAC GTGGCTATCG CCACCCTCGA AAAGAAGCTG 
CTCAGCCAGC AGGAGGAGCT CACAGAGCTA CACAAGCGCA AGGGCGAGAA CTCCCAGATG 
ATCGTGGACC TCAACCAGAA GGTCGAGCAG CAGAGGATCA TAATTTCCGA GAAGGAGCAC 
AGCCTCGTCG AACAGCAAAC GAACAACAAC CGATTGCGCG CGGAGGTGCA GCTACTTCAC 
TCGAGTTTGG AGGAACTCAA GAAGCTGAAC AACACAATGC TGGACGAACA TACGGCGCTT 
CAATTGGCAT TTAGCTCCCT CGAGGAGAAG TTAAGGGGAG TGCAAGATGA AAATCGGCGT 
CTACTGGAGC GACTTATGCA ATACAAGTCA AAGGATGCCG ACAAGTTGAA CGAGGAAAAC 
GAGAGCATAA TTAGAAAAAG ACTGCCGTCA ATTTTCAGGA AACGTTCGGC TAAACTGAAA 
CGTGACCTAG AAGACGCGGT TCGTGAGCCC AGTTCGTCCA GCAACGCCGC CTCCTCGCCC 
GGCGCCGCAT CCTTGCAGCG CAACTCCAGT CCCGCCCAGT TCGTTGGCGG CCTCATCGGC 
GACGAGGATT TCGACGAGGC CGCGATAAAT GGCGCCATGG AGGCCATAGG TCTCGATGAT 
AATGAATATA TTAGCGCTCG TTTTACAGCA GGCGAAATCG CCGAGAACTC GCGGGCTTCC 
ATCGACACCC TGAAGGCCAC GGGCTACCTG GGCCAGGCCA ATCCCACCAA GATCCTCATG 
AAGTTCGAGG CCCACGAGAA CGAGTCGCAC GCGGTGCGCT GGAGTCCCGT GGAGCGCATG 
GTGGCCACCG GTGGCGCTGA TCGCAAAGTC AAACTTTGGG ACATTGGCAA AAATTCCACC 
GAACCACGTG CCGTTCTAAG CGGGAGCAGT GCTGGGATTA ACTCCGTAGA TTTCGATTCG 
ACAGGCGCCT ACATCCTGGG CACCTCCAAT GACTACGGAG CCAGAGTATG GACAGTGATG 
GACAATCGCT TAAGACACAC GCTGACGGGT CACAGCGGCA AGGTAATGGC CGCCAAGTAC 
GTCCAGGAGC CCATCAAAGT GGTGACCGGT AGCCACGATC GCACACTGAA GATCTGGGAT 
TTGCGCAGCA TCGCCTGCAT AGAGACGAAG TTCGCCGGCT CCAGCTGCAA TGATCTAGTC 
ACCACCGACA GCCTCGGATC AACGATCATC AGTGGGCACT ACGATAAGAA GATCCGGTTC 
TGGGACATAC GCACCGAGAA GCAGGCGGAC GACGTCTTAA TGCCGGCCAA GATCACCTCT 
TTGGATTTGT CAAAAGATTG CAATTACCTC ATATGCTCTG TAAGAGACGA TACTATTAAA 
CTGTTAGATT TACGCAAGAA CCAAGTTATA TCCACGTTTA CAAACGAACA CTTTAAGATC 
AGCTGTGATT TTGCTCGAGC TTCTTTCAAC TCGAGCGGCC TAAAGATCGC TTGTGGATCA 
GCGGATGGCG CTATATACAT TTGGAATGTG AATGGGTTCC TGGAAGCCAC ACTCAAGGGA 
CACAGCACCG CCGTAAACGC GGTCAGTTGG AGTCCCAACA ACAACATGCT GGCCTCCGTG 
GGAAAGAACA AGCGCTGCAC CATCTACTCG GAGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Atg16 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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