Rhp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rhp
Entrez Gene ID	32531
Full Name	Rhophilin
Synonyms	CG8497,Dmel\CG8497
General protein information	Preferred Names Rhophilin Names CG8497 gene product from transcript CG8497-RB CG8497-PA CG8497-PB Rhp-PA Rhp-PB rhophilin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 13E18-13E18

mRNA and Protein(s)

mRNA	Protein	Name
NM_078613.4	NP_511168.3	rhophilin, isoform A
NM_206724.2	NP_996447.1	rhophilin, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RHPN2	XP_001152684.1
Bos taurus (cattle)	RHPN2	NP_001076939.1
Xenopus tropicalis (tropical clawed frog)	rhpn2	NP_001120536.1
Homo sapiens (human)	RHPN2	NP_149094.3
Danio rerio (zebrafish)	rhpn2	NP_957472.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000243	XP_310883.5
Gallus gallus (chicken)	RHPN2	XP_414133.2
Mus musculus (house mouse)	Rhpn2	NP_082173.3
Drosophila melanogaster (fruit fly)	Rhp	NP_511168.3
Canis lupus familiaris (dog)	RHPN2	NP_001003008.1
Rattus norvegicus (Norway rat)	Rhpn2	NP_001100975.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rhp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rhp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03607	NM_206724.2 Latest version!	Drosophila melanogaster rhophilin, transcript variant B (Rhp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OFa03607	NM_078613.4 Latest version!	Drosophila melanogaster rhophilin, transcript variant A (Rhp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rhp ( NM_078613.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511168.3 Drosophila melanogaster rhophilin, transcript variant A (Rhp), mRNA.

Price & Availability↑

CloneID	OFa03607
Clone ID Related Accession (Same CDS sequence)	NM_078613.4 , NM_206724.2
Accession Version	NM_078613.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	rhophilin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078613.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGAAA AGGAACACGA ATTGTCGACG AGGGTGGTGA AAAACGAGGA GAACGAGGAT 
GACGAGAAGC TCAATGAGTT ATCCTTTTGT TTTGTTCGGG GTTCAGATCC ACGAGCTGCC 
ACTTGTCGTA GCAAACTCCA AAACAGGCGA TGCAAATTAA ACAAAGAAAT AAATAAGGAA 
CTCCGCCTTC GAGCTGGTGC CGAGAATCTA TATAAGGCCA CATCGAATCG CAAACTGCGC 
GACACAGTTG CCTTGGAATT AAGCTTTGTC AACTCAAATC TGCAACTGCT GAAGGAGCAA 
CTGGCCGAGT TGAATTCGTC GGTGGAAATC TATCAGAGCG AAAGTCACAA TGGAATTATG 
CCCATGATAC CGCTGGGCCT GAAGGAAACC AAGGAGATCA ATTTCATGGA ACCCTTCTCC 
GACTTCATCC TGGAACACTA CAGCGAGGAG CCCTCGATGT ACATAGATGC CATAGCTGAT 
ATGACGGACA CGAGACAGGC ATCCAAAACA CCGTCACGAG ATGCCCTGGG AGTGGCCCTA 
CTTTTCCGCT ACTACAACAC GCTGTATTAC GTGGAGCGTC GCTTCTTTCC GCCGGATCGT 
AACCTGGGCG TGTACTTCGA GTGGTACGAC TCGCTCACGG GAGTTCCCTC GTGCCAGCGG 
ACCATCGCGT TCGAGAAAGC CTGCACCCTG TTCAATCTGG GCGGCATATA TACACAGATC 
GGCGCTCGAC ACGATCGCAC GACGGAACGG GGTCTTGACT TGGCCGTGGA TAGTTTTTTA 
CGGGCCGCCG GGGTCTTTCG TCACATCTAC GATACGTTCA CGAATGCACC ATCGATGGAC 
CTGAAGCCAC AGGTCCTGGA CGTGCTCGTT TCCCTGATGC TTTCCCAGGC ACGAGAATGC 
CTCTTTGAGA AGCTTCAGCT GCAGATCGAG GCGATGAGCC ACGATTGCCA GGCGTTTCGG 
GATCTGGCTG GCGAGGCTGC TCAAATATCG CACGAGTACA ATGAGATGCA CAAGAATATC 
CAGGCGAACG ATACGCACAC CTATCTTCCG GAATGCTGGG CCGGTCTAGT GCCAGTCAAG 
GCGGAACTCT ACAAAGCATT CGCACATTTC TACAAGGCAC GGAGCATAGA TGCGACAGAC 
GAGCTGAAGG CATCAAAGTC AAGTCAAAAG AACCAAGAAT CCTTCATCGG CAATTCCCAG 
GAGGTGGAGC GCATCACCAC AGCCGATTAT GGAGCCAGCG ATGAGGCGTC CACCTCCATT 
GCCAACAAAC TGGCCCACTT AAAGGAGGCG CTGGCCAGTA TCGAGGAAGC CCAGCGATTG 
CAGCGGATGT GTCGGTTCTT GAAGAACAAG GCATCACTGA CTGAAGTCAT GAAGGAGGTG 
CACTCCAAAT CGCAGGAGGA GCTCGAGAAG TTCAGACTGC AAGCTTCAGC CAAAAATATC 
GAAGATGGCG ATCTTCTAGA GCGATCGGTA GAAGCATCTT CGAAGTTCAC GTTATCCCTG 
ACCGGACCCG ACTTCACATC GCACAAGGTT AAGGATCCCT TCAAGCGCCT CGGCCCGATT 
GCAATATTCT CGGCGAGACG CCACTGGACA GCGCCCAGAT GTGTGCGTCT CCAGAAAGGT 
TCCTCCCTGT ATCACAGCGT GCCCAGCAAT GACAACAAGT GCCCATTGGA TAACGATGAT 
GACGAGGAGC ACGATGGTGG ATACAATCTG TACAAGGAGG AGTTTGAGAA CTTTGGCTTC 
CATGTGCGCG GCGATGCTCC GGTGATCATT GCCCACGTTG AGATTAATTC TTTGGCAGAC 
CTTGGCGGCA TCAAGGAGGG AGACTTCATA GTCGAGATAG CCGGCGTGGA CGTGAAGTGG 
TACTCCCATC AGCAGGTGGT GCAGCTCATA CAATCCTGCG GCTCTACACT CGAGCTGAGA 
GTGATAACGC CCATGGATCG CAACTACTTG AAGCCATTGT CGTCGAAGGG CTCATTATCA 
ACGCTATCGG CGGCCAGTTC ATCGGGCATT TCATCCGGAT TTCCCAGTCC CACAAGTATT 
GCGGCCAAGC CCAAGCTCCA CCTGAAAACA TCCTCTAGTT CGCGACCCGC TGGCAGCGTT 
TCATCCAGTT CGTGGAATCC GTTTCGGCGA ACGCCTAGCT TAGCTAAAAT ATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511168.3
CDS	105..2261
Translation	MDEKEHELSTRVVKNEENEDDEKLNELSFCFVRGSDPRAATCRSKLQNRRCKLNKEINKELRLRAGAENLYKATSNRKLRDTVALELSFVNSNLQLLKEQLAELNSSVEIYQSESHNGIMPMIPLGLKETKEINFMEPFSDFILEHYSEEPSMYIDAIADMTDTRQASKTPSRDALGVALLFRYYNTLYYVERRFFPPDRNLGVYFEWYDSLTGVPSCQRTIAFEKACTLFNLGGIYTQIGARHDRTTERGLDLAVDSFLRAAGVFRHIYDTFTNAPSMDLKPQVLDVLVSLMLSQARECLFEKLQLQIEAMSHDCQAFRDLAGEAAQISHEYNEMHKNIQANDTHTYLPECWAGLVPVKAELYKAFAHFYKARSIDATDELKASKSSQKNQESFIGNSQEVERITTADYGASDEASTSIANKLAHLKEALASIEEAQRLQRMCRFLKNKASLTEVMKEVHSKSQEELEKFRLQASAKNIEDGDLLERSVEASSKFTLSLTGPDFTSHKVKDPFKRLGPIAIFSARRHWTAPRCVRLQKGSSLYHSVPSNDNKCPLDNDDDEEHDGGYNLYKEEFENFGFHVRGDAPVIIAHVEINSLADLGGIKEGDFIVEIAGVDVKWYSHQQVVQLIQSCGSTLELRVITPMDRNYLKPLSSKGSLSTLSAASSSGISSGFPSPTSIAAKPKLHLKTSSSSRPAGSVSSSSWNPFRRTPSLAKIF

Target ORF information:

RefSeq Version	NM_078613.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rhophilin, transcript variant A (Rhp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078613.4

ORF Insert Sequence:

ATGGATGAAA AGGAACACGA ATTGTCGACG AGGGTGGTGA AAAACGAGGA GAACGAGGAT 
GACGAGAAGC TCAATGAGTT ATCCTTTTGT TTTGTTCGGG GTTCAGATCC ACGAGCTGCC 
ACTTGTCGTA GCAAACTCCA AAACAGGCGA TGCAAATTAA ACAAAGAAAT AAATAAGGAA 
CTCCGCCTTC GAGCTGGTGC CGAGAATCTA TATAAGGCCA CATCGAATCG CAAACTGCGC 
GACACAGTTG CCTTGGAATT AAGCTTTGTC AACTCAAATC TGCAACTGCT GAAGGAGCAA 
CTGGCCGAGT TGAATTCGTC GGTGGAAATC TATCAGAGCG AAAGTCACAA TGGAATTATG 
CCCATGATAC CGCTGGGCCT GAAGGAAACC AAGGAGATCA ATTTCATGGA ACCCTTCTCC 
GACTTCATCC TGGAACACTA CAGCGAGGAG CCCTCGATGT ACATAGATGC CATAGCTGAT 
ATGACGGACA CGAGACAGGC ATCCAAAACA CCGTCACGAG ATGCCCTGGG AGTGGCCCTA 
CTTTTCCGCT ACTACAACAC GCTGTATTAC GTGGAGCGTC GCTTCTTTCC GCCGGATCGT 
AACCTGGGCG TGTACTTCGA GTGGTACGAC TCGCTCACGG GAGTTCCCTC GTGCCAGCGG 
ACCATCGCGT TCGAGAAAGC CTGCACCCTG TTCAATCTGG GCGGCATATA TACACAGATC 
GGCGCTCGAC ACGATCGCAC GACGGAACGG GGTCTTGACT TGGCCGTGGA TAGTTTTTTA 
CGGGCCGCCG GGGTCTTTCG TCACATCTAC GATACGTTCA CGAATGCACC ATCGATGGAC 
CTGAAGCCAC AGGTCCTGGA CGTGCTCGTT TCCCTGATGC TTTCCCAGGC ACGAGAATGC 
CTCTTTGAGA AGCTTCAGCT GCAGATCGAG GCGATGAGCC ACGATTGCCA GGCGTTTCGG 
GATCTGGCTG GCGAGGCTGC TCAAATATCG CACGAGTACA ATGAGATGCA CAAGAATATC 
CAGGCGAACG ATACGCACAC CTATCTTCCG GAATGCTGGG CCGGTCTAGT GCCAGTCAAG 
GCGGAACTCT ACAAAGCATT CGCACATTTC TACAAGGCAC GGAGCATAGA TGCGACAGAC 
GAGCTGAAGG CATCAAAGTC AAGTCAAAAG AACCAAGAAT CCTTCATCGG CAATTCCCAG 
GAGGTGGAGC GCATCACCAC AGCCGATTAT GGAGCCAGCG ATGAGGCGTC CACCTCCATT 
GCCAACAAAC TGGCCCACTT AAAGGAGGCG CTGGCCAGTA TCGAGGAAGC CCAGCGATTG 
CAGCGGATGT GTCGGTTCTT GAAGAACAAG GCATCACTGA CTGAAGTCAT GAAGGAGGTG 
CACTCCAAAT CGCAGGAGGA GCTCGAGAAG TTCAGACTGC AAGCTTCAGC CAAAAATATC 
GAAGATGGCG ATCTTCTAGA GCGATCGGTA GAAGCATCTT CGAAGTTCAC GTTATCCCTG 
ACCGGACCCG ACTTCACATC GCACAAGGTT AAGGATCCCT TCAAGCGCCT CGGCCCGATT 
GCAATATTCT CGGCGAGACG CCACTGGACA GCGCCCAGAT GTGTGCGTCT CCAGAAAGGT 
TCCTCCCTGT ATCACAGCGT GCCCAGCAAT GACAACAAGT GCCCATTGGA TAACGATGAT 
GACGAGGAGC ACGATGGTGG ATACAATCTG TACAAGGAGG AGTTTGAGAA CTTTGGCTTC 
CATGTGCGCG GCGATGCTCC GGTGATCATT GCCCACGTTG AGATTAATTC TTTGGCAGAC 
CTTGGCGGCA TCAAGGAGGG AGACTTCATA GTCGAGATAG CCGGCGTGGA CGTGAAGTGG 
TACTCCCATC AGCAGGTGGT GCAGCTCATA CAATCCTGCG GCTCTACACT CGAGCTGAGA 
GTGATAACGC CCATGGATCG CAACTACTTG AAGCCATTGT CGTCGAAGGG CTCATTATCA 
ACGCTATCGG CGGCCAGTTC ATCGGGCATT TCATCCGGAT TTCCCAGTCC CACAAGTATT 
GCGGCCAAGC CCAAGCTCCA CCTGAAAACA TCCTCTAGTT CGCGACCCGC TGGCAGCGTT 
TCATCCAGTT CGTGGAATCC GTTTCGGCGA ACGCCTAGCT TAGCTAAAAT ATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhp ( NM_206724.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996447.1 Drosophila melanogaster rhophilin, transcript variant B (Rhp), mRNA.

Price & Availability↑

CloneID	OFa03607
Clone ID Related Accession (Same CDS sequence)	NM_078613.4 , NM_206724.2
Accession Version	NM_206724.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	rhophilin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206724.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGAAA AGGAACACGA ATTGTCGACG AGGGTGGTGA AAAACGAGGA GAACGAGGAT 
GACGAGAAGC TCAATGAGTT ATCCTTTTGT TTTGTTCGGG GTTCAGATCC ACGAGCTGCC 
ACTTGTCGTA GCAAACTCCA AAACAGGCGA TGCAAATTAA ACAAAGAAAT AAATAAGGAA 
CTCCGCCTTC GAGCTGGTGC CGAGAATCTA TATAAGGCCA CATCGAATCG CAAACTGCGC 
GACACAGTTG CCTTGGAATT AAGCTTTGTC AACTCAAATC TGCAACTGCT GAAGGAGCAA 
CTGGCCGAGT TGAATTCGTC GGTGGAAATC TATCAGAGCG AAAGTCACAA TGGAATTATG 
CCCATGATAC CGCTGGGCCT GAAGGAAACC AAGGAGATCA ATTTCATGGA ACCCTTCTCC 
GACTTCATCC TGGAACACTA CAGCGAGGAG CCCTCGATGT ACATAGATGC CATAGCTGAT 
ATGACGGACA CGAGACAGGC ATCCAAAACA CCGTCACGAG ATGCCCTGGG AGTGGCCCTA 
CTTTTCCGCT ACTACAACAC GCTGTATTAC GTGGAGCGTC GCTTCTTTCC GCCGGATCGT 
AACCTGGGCG TGTACTTCGA GTGGTACGAC TCGCTCACGG GAGTTCCCTC GTGCCAGCGG 
ACCATCGCGT TCGAGAAAGC CTGCACCCTG TTCAATCTGG GCGGCATATA TACACAGATC 
GGCGCTCGAC ACGATCGCAC GACGGAACGG GGTCTTGACT TGGCCGTGGA TAGTTTTTTA 
CGGGCCGCCG GGGTCTTTCG TCACATCTAC GATACGTTCA CGAATGCACC ATCGATGGAC 
CTGAAGCCAC AGGTCCTGGA CGTGCTCGTT TCCCTGATGC TTTCCCAGGC ACGAGAATGC 
CTCTTTGAGA AGCTTCAGCT GCAGATCGAG GCGATGAGCC ACGATTGCCA GGCGTTTCGG 
GATCTGGCTG GCGAGGCTGC TCAAATATCG CACGAGTACA ATGAGATGCA CAAGAATATC 
CAGGCGAACG ATACGCACAC CTATCTTCCG GAATGCTGGG CCGGTCTAGT GCCAGTCAAG 
GCGGAACTCT ACAAAGCATT CGCACATTTC TACAAGGCAC GGAGCATAGA TGCGACAGAC 
GAGCTGAAGG CATCAAAGTC AAGTCAAAAG AACCAAGAAT CCTTCATCGG CAATTCCCAG 
GAGGTGGAGC GCATCACCAC AGCCGATTAT GGAGCCAGCG ATGAGGCGTC CACCTCCATT 
GCCAACAAAC TGGCCCACTT AAAGGAGGCG CTGGCCAGTA TCGAGGAAGC CCAGCGATTG 
CAGCGGATGT GTCGGTTCTT GAAGAACAAG GCATCACTGA CTGAAGTCAT GAAGGAGGTG 
CACTCCAAAT CGCAGGAGGA GCTCGAGAAG TTCAGACTGC AAGCTTCAGC CAAAAATATC 
GAAGATGGCG ATCTTCTAGA GCGATCGGTA GAAGCATCTT CGAAGTTCAC GTTATCCCTG 
ACCGGACCCG ACTTCACATC GCACAAGGTT AAGGATCCCT TCAAGCGCCT CGGCCCGATT 
GCAATATTCT CGGCGAGACG CCACTGGACA GCGCCCAGAT GTGTGCGTCT CCAGAAAGGT 
TCCTCCCTGT ATCACAGCGT GCCCAGCAAT GACAACAAGT GCCCATTGGA TAACGATGAT 
GACGAGGAGC ACGATGGTGG ATACAATCTG TACAAGGAGG AGTTTGAGAA CTTTGGCTTC 
CATGTGCGCG GCGATGCTCC GGTGATCATT GCCCACGTTG AGATTAATTC TTTGGCAGAC 
CTTGGCGGCA TCAAGGAGGG AGACTTCATA GTCGAGATAG CCGGCGTGGA CGTGAAGTGG 
TACTCCCATC AGCAGGTGGT GCAGCTCATA CAATCCTGCG GCTCTACACT CGAGCTGAGA 
GTGATAACGC CCATGGATCG CAACTACTTG AAGCCATTGT CGTCGAAGGG CTCATTATCA 
ACGCTATCGG CGGCCAGTTC ATCGGGCATT TCATCCGGAT TTCCCAGTCC CACAAGTATT 
GCGGCCAAGC CCAAGCTCCA CCTGAAAACA TCCTCTAGTT CGCGACCCGC TGGCAGCGTT 
TCATCCAGTT CGTGGAATCC GTTTCGGCGA ACGCCTAGCT TAGCTAAAAT ATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996447.1
CDS	260..2416
Translation	MDEKEHELSTRVVKNEENEDDEKLNELSFCFVRGSDPRAATCRSKLQNRRCKLNKEINKELRLRAGAENLYKATSNRKLRDTVALELSFVNSNLQLLKEQLAELNSSVEIYQSESHNGIMPMIPLGLKETKEINFMEPFSDFILEHYSEEPSMYIDAIADMTDTRQASKTPSRDALGVALLFRYYNTLYYVERRFFPPDRNLGVYFEWYDSLTGVPSCQRTIAFEKACTLFNLGGIYTQIGARHDRTTERGLDLAVDSFLRAAGVFRHIYDTFTNAPSMDLKPQVLDVLVSLMLSQARECLFEKLQLQIEAMSHDCQAFRDLAGEAAQISHEYNEMHKNIQANDTHTYLPECWAGLVPVKAELYKAFAHFYKARSIDATDELKASKSSQKNQESFIGNSQEVERITTADYGASDEASTSIANKLAHLKEALASIEEAQRLQRMCRFLKNKASLTEVMKEVHSKSQEELEKFRLQASAKNIEDGDLLERSVEASSKFTLSLTGPDFTSHKVKDPFKRLGPIAIFSARRHWTAPRCVRLQKGSSLYHSVPSNDNKCPLDNDDDEEHDGGYNLYKEEFENFGFHVRGDAPVIIAHVEINSLADLGGIKEGDFIVEIAGVDVKWYSHQQVVQLIQSCGSTLELRVITPMDRNYLKPLSSKGSLSTLSAASSSGISSGFPSPTSIAAKPKLHLKTSSSSRPAGSVSSSSWNPFRRTPSLAKIF

Target ORF information:

RefSeq Version	NM_206724.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rhophilin, transcript variant B (Rhp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206724.2

ORF Insert Sequence:

ATGGATGAAA AGGAACACGA ATTGTCGACG AGGGTGGTGA AAAACGAGGA GAACGAGGAT 
GACGAGAAGC TCAATGAGTT ATCCTTTTGT TTTGTTCGGG GTTCAGATCC ACGAGCTGCC 
ACTTGTCGTA GCAAACTCCA AAACAGGCGA TGCAAATTAA ACAAAGAAAT AAATAAGGAA 
CTCCGCCTTC GAGCTGGTGC CGAGAATCTA TATAAGGCCA CATCGAATCG CAAACTGCGC 
GACACAGTTG CCTTGGAATT AAGCTTTGTC AACTCAAATC TGCAACTGCT GAAGGAGCAA 
CTGGCCGAGT TGAATTCGTC GGTGGAAATC TATCAGAGCG AAAGTCACAA TGGAATTATG 
CCCATGATAC CGCTGGGCCT GAAGGAAACC AAGGAGATCA ATTTCATGGA ACCCTTCTCC 
GACTTCATCC TGGAACACTA CAGCGAGGAG CCCTCGATGT ACATAGATGC CATAGCTGAT 
ATGACGGACA CGAGACAGGC ATCCAAAACA CCGTCACGAG ATGCCCTGGG AGTGGCCCTA 
CTTTTCCGCT ACTACAACAC GCTGTATTAC GTGGAGCGTC GCTTCTTTCC GCCGGATCGT 
AACCTGGGCG TGTACTTCGA GTGGTACGAC TCGCTCACGG GAGTTCCCTC GTGCCAGCGG 
ACCATCGCGT TCGAGAAAGC CTGCACCCTG TTCAATCTGG GCGGCATATA TACACAGATC 
GGCGCTCGAC ACGATCGCAC GACGGAACGG GGTCTTGACT TGGCCGTGGA TAGTTTTTTA 
CGGGCCGCCG GGGTCTTTCG TCACATCTAC GATACGTTCA CGAATGCACC ATCGATGGAC 
CTGAAGCCAC AGGTCCTGGA CGTGCTCGTT TCCCTGATGC TTTCCCAGGC ACGAGAATGC 
CTCTTTGAGA AGCTTCAGCT GCAGATCGAG GCGATGAGCC ACGATTGCCA GGCGTTTCGG 
GATCTGGCTG GCGAGGCTGC TCAAATATCG CACGAGTACA ATGAGATGCA CAAGAATATC 
CAGGCGAACG ATACGCACAC CTATCTTCCG GAATGCTGGG CCGGTCTAGT GCCAGTCAAG 
GCGGAACTCT ACAAAGCATT CGCACATTTC TACAAGGCAC GGAGCATAGA TGCGACAGAC 
GAGCTGAAGG CATCAAAGTC AAGTCAAAAG AACCAAGAAT CCTTCATCGG CAATTCCCAG 
GAGGTGGAGC GCATCACCAC AGCCGATTAT GGAGCCAGCG ATGAGGCGTC CACCTCCATT 
GCCAACAAAC TGGCCCACTT AAAGGAGGCG CTGGCCAGTA TCGAGGAAGC CCAGCGATTG 
CAGCGGATGT GTCGGTTCTT GAAGAACAAG GCATCACTGA CTGAAGTCAT GAAGGAGGTG 
CACTCCAAAT CGCAGGAGGA GCTCGAGAAG TTCAGACTGC AAGCTTCAGC CAAAAATATC 
GAAGATGGCG ATCTTCTAGA GCGATCGGTA GAAGCATCTT CGAAGTTCAC GTTATCCCTG 
ACCGGACCCG ACTTCACATC GCACAAGGTT AAGGATCCCT TCAAGCGCCT CGGCCCGATT 
GCAATATTCT CGGCGAGACG CCACTGGACA GCGCCCAGAT GTGTGCGTCT CCAGAAAGGT 
TCCTCCCTGT ATCACAGCGT GCCCAGCAAT GACAACAAGT GCCCATTGGA TAACGATGAT 
GACGAGGAGC ACGATGGTGG ATACAATCTG TACAAGGAGG AGTTTGAGAA CTTTGGCTTC 
CATGTGCGCG GCGATGCTCC GGTGATCATT GCCCACGTTG AGATTAATTC TTTGGCAGAC 
CTTGGCGGCA TCAAGGAGGG AGACTTCATA GTCGAGATAG CCGGCGTGGA CGTGAAGTGG 
TACTCCCATC AGCAGGTGGT GCAGCTCATA CAATCCTGCG GCTCTACACT CGAGCTGAGA 
GTGATAACGC CCATGGATCG CAACTACTTG AAGCCATTGT CGTCGAAGGG CTCATTATCA 
ACGCTATCGG CGGCCAGTTC ATCGGGCATT TCATCCGGAT TTCCCAGTCC CACAAGTATT 
GCGGCCAAGC CCAAGCTCCA CCTGAAAACA TCCTCTAGTT CGCGACCCGC TGGCAGCGTT 
TCATCCAGTT CGTGGAATCC GTTTCGGCGA ACGCCTAGCT TAGCTAAAAT ATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Rhp cDNA ORF clone, Drosophila melanogaster(fruit fly)

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