Syt12 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Syt12
Entrez Gene ID	32290
Full Name	Synaptotagmin 12
Synonyms	CG10617,Dmel\CG10617,Syt 12,dsyt12,syt12
General protein information	Preferred Names Synaptotagmin 12 Names CG10617 gene product from transcript CG10617-RA CG10617-PA CG10617-PB Syt12-PA Syt12-PB synaptotagmin 12 syt12
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 11F1-11F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_132650.2	NP_572878.1	synaptotagmin 12, isoform A
NM_001272584.1	NP_001259513.1	synaptotagmin 12, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Syt12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Syt12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01488	NM_132650.2 Latest version!	Drosophila melanogaster synaptotagmin 12, transcript variant A (Syt12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OFa01488	NM_001272584.1 Latest version!	Drosophila melanogaster synaptotagmin 12, transcript variant B (Syt12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Syt12 ( NM_132650.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572878.1 Drosophila melanogaster synaptotagmin 12, transcript variant A (Syt12), mRNA.

Price & Availability↑

CloneID	OFa01488
Clone ID Related Accession (Same CDS sequence)	NM_132650.2 , NM_001272584.1
Accession Version	NM_132650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	synaptotagmin 12, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132650.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCT TCACCATTAC CTTGGCGCTA CTGCTCCTGG CAGTTCTGAT CCTGGGCATC 
TTGTTCGCCT GCCACTGTCT TGGTCCGCGG GCCGCCAACT GGATGCGTAT GCGATCCAGC 
AAGGAGGAAA AGATCGGACT ATCCAATCAC AAGCAAAAGC TCTTCCATGC CAATGGTTAT 
ATGGCCAGTA TACAATCCGG CTCCGAGTTC CTGCTGAGCA CCAGTGGTAG TTTCAAGCGT 
TTCGATACCA TCGACAAGGA GGACTACAAT CGCTCCCAGC AGACGCATCA GACGCATCTG 
TTCCTGATGA ACGGCGGCGG TGCGGTTTCG ATAACCGGAT CATCCAGTGC CCTGCAGGCC 
ACCACGGCCA TGTGGCATCT ACCGCGTTCG GGTATTAAGA TTCCACCACC GCCCGATCGA 
CCAGCTCCAT CTCCGAATAG TGCTGCAGCA GCCGGCAAAA CGGTTACCTT CTCTTTGAGC 
CAGGTAAACG AGGTCATCAT GCCCGGCGAG CAGGTGACAT CCAACGATCA GGAACGTCGC 
TCCTGCCTGC GACAAAATGG TGGAACTGCG GGCGGTGCTG GCGGCGGTGG TGTGCTGCCG 
CAGCCACTGG AGGCACCACC TCCGCCACGA CTCACCGCAT CGACGTCGGT GAACTTATCG 
GGTACGGCGA TTCCAAGGCC CATTGCCAAG CGGCAAGTGT CCACGCAGCC GGCCAACAAT 
AGCGGATGCC AGCCGGAGCA GACCGTGGTG ACCCCGATGC GGGAGGGTGA GAGAAAAGCG 
CCTGCCTTGA AGCGTAGAAA CTCCAGCACT AATCCCTTCC TGTGCGAATC AACGGAGCAG 
ATACCCAGTA ATCCCATTTC TGAACCTCTG AATGCCACGG TGGCTTCTCT GGAAAAAACG 
ACTATTTCAA CAAGTGAATG TACTCCTATA ACTGCTCCTC TAGAGACTCC AAATGGGCCT 
ATTTCAACGG ATACTCCATC CACCATACAT CACAATGGCA ATCCCTTCGT GGAGAGAGAA 
AGTCTGTCCA GCAGACTCCT GAAGATCCAT AACACAACGA ATCCTTTCAC GGGACTCTCG 
CAACGCGTCA AGGGTCCGCA TCTACTGCAA AAGACCATTT CGGAGGATTA TCTATTTCGG 
AAATTGGGTG TCAATTGTCC GATGGCCAAT GGCAATGGTA CGGGACCAGG ATCTGGTCAT 
GTCAACGGAA ATGGCAATGG AACTTGGTCC TTTGGACGCT CCCTGTTGCG ACAGGACTCC 
ACATTGAGTC TGGGCATGGG CTTGGGCAGA AGGAACAGCT CCCAGGTATC CCTGGATTCA 
CATGCGGGAT CGGTGCCCGG TTCAATGGAT GGCATCAATT TGGAGCGAGC CATCTCCTGC 
GATTCGGTAA CCTCGGACTC CACTCTATTC CTGGATCAAT TGGATCAGCC CTACACTCAG 
ATAACCGGAT ATTTATGCGT TGGCCTTAAC TACGATCAGA TGAGTATTAG CAACGAGGGC 
ATGGAACTGA CTGTTAGTGT CCTGGAGGCC AAGGGACTCA TATGTCCATT CAGCGTTGAA 
TCCCTGGACA CCTTTGTGCG CATCTACTTG GTGCCGGATC ATCCGGGAGC TATGCAAACT 
AAGGTGGTCA AGGGAACACT GACACCCAGC TACAACGAAA GTTTCGATTT CTGGCTGCAC 
AAGCGACAGG CGCGCCATTC GCTGTGGTTC CATCTGTACC ACAATGGACC CGCCCACACG 
CTCATTGGCG AGGCGGAGAT GGAAATTGGT GAGATGCCAC GCCCCATTAC CACATGGATA 
CCACTGTCCG ATTCGCGCAA GTGCAACGCC CGCTGGGGCG AGCTAATGTT CTCTCTAAGC 
TATCTGCCCA CGGCGGAAAG ACTAACCATC GTCGTGGTCA AGGCACGGAA TCTCAAGCTG 
GACGGTGAGC AACCGGCTCC GGAATCCGCG GAAACGGTGC ACAGCGTCTT CGTTAAGGTC 
TACCTGATGG ACAAGGATCG CAAGGTGCTA AAGAAGCGCA CCTCACTGAA GCGCAAGGAT 
CGCAGTCCGA TCTTCAACGA ATCGATGATC TTCAGTGTGC CGCCACCCAG TTTGACCACC 
ACCCAGCTGC GGGTGACCGT GTTCGGGGTG ACAACTAGTG GGGTGACCCC GTTGGGTCAC 
ATCGTGGCCG GCAGCTGTGC GGTGGGCAAG GGATTGCGCC ATTGGCACCA GATGCTCTCG 
TCGCTTAGGA AACCGGTGGC CATGTGGCAT GTCCTGCGGC GTGCGGTCAA TCAGCCGATT 
TTCACGGGCG GCGCCGAGGC GGTGGTGGCC GCCATGCAAA ACACCCTGCA ACGCTCCACC 
GCCAAGCGGA ACAGCATCGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572878.1
CDS	242..2605
Translation	MMSFTITLALLLLAVLILGILFACHCLGPRAANWMRMRSSKEEKIGLSNHKQKLFHANGYMASIQSGSEFLLSTSGSFKRFDTIDKEDYNRSQQTHQTHLFLMNGGGAVSITGSSSALQATTAMWHLPRSGIKIPPPPDRPAPSPNSAAAAGKTVTFSLSQVNEVIMPGEQVTSNDQERRSCLRQNGGTAGGAGGGGVLPQPLEAPPPPRLTASTSVNLSGTAIPRPIAKRQVSTQPANNSGCQPEQTVVTPMREGERKAPALKRRNSSTNPFLCESTEQIPSNPISEPLNATVASLEKTTISTSECTPITAPLETPNGPISTDTPSTIHHNGNPFVERESLSSRLLKIHNTTNPFTGLSQRVKGPHLLQKTISEDYLFRKLGVNCPMANGNGTGPGSGHVNGNGNGTWSFGRSLLRQDSTLSLGMGLGRRNSSQVSLDSHAGSVPGSMDGINLERAISCDSVTSDSTLFLDQLDQPYTQITGYLCVGLNYDQMSISNEGMELTVSVLEAKGLICPFSVESLDTFVRIYLVPDHPGAMQTKVVKGTLTPSYNESFDFWLHKRQARHSLWFHLYHNGPAHTLIGEAEMEIGEMPRPITTWIPLSDSRKCNARWGELMFSLSYLPTAERLTIVVVKARNLKLDGEQPAPESAETVHSVFVKVYLMDKDRKVLKKRTSLKRKDRSPIFNESMIFSVPPPSLTTTQLRVTVFGVTTSGVTPLGHIVAGSCAVGKGLRHWHQMLSSLRKPVAMWHVLRRAVNQPIFTGGAEAVVAAMQNTLQRSTAKRNSIV

Target ORF information:

RefSeq Version	NM_132650.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster synaptotagmin 12, transcript variant A (Syt12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132650.2

ORF Insert Sequence:

ATGATGAGCT TCACCATTAC CTTGGCGCTA CTGCTCCTGG CAGTTCTGAT CCTGGGCATC 
TTGTTCGCCT GCCACTGTCT TGGTCCGCGG GCCGCCAACT GGATGCGTAT GCGATCCAGC 
AAGGAGGAAA AGATCGGACT ATCCAATCAC AAGCAAAAGC TCTTCCATGC CAATGGTTAT 
ATGGCCAGTA TACAATCCGG CTCCGAGTTC CTGCTGAGCA CCAGTGGTAG TTTCAAGCGT 
TTCGATACCA TCGACAAGGA GGACTACAAT CGCTCCCAGC AGACGCATCA GACGCATCTG 
TTCCTGATGA ACGGCGGCGG TGCGGTTTCG ATAACCGGAT CATCCAGTGC CCTGCAGGCC 
ACCACGGCCA TGTGGCATCT ACCGCGTTCG GGTATTAAGA TTCCACCACC GCCCGATCGA 
CCAGCTCCAT CTCCGAATAG TGCTGCAGCA GCCGGCAAAA CGGTTACCTT CTCTTTGAGC 
CAGGTAAACG AGGTCATCAT GCCCGGCGAG CAGGTGACAT CCAACGATCA GGAACGTCGC 
TCCTGCCTGC GACAAAATGG TGGAACTGCG GGCGGTGCTG GCGGCGGTGG TGTGCTGCCG 
CAGCCACTGG AGGCACCACC TCCGCCACGA CTCACCGCAT CGACGTCGGT GAACTTATCG 
GGTACGGCGA TTCCAAGGCC CATTGCCAAG CGGCAAGTGT CCACGCAGCC GGCCAACAAT 
AGCGGATGCC AGCCGGAGCA GACCGTGGTG ACCCCGATGC GGGAGGGTGA GAGAAAAGCG 
CCTGCCTTGA AGCGTAGAAA CTCCAGCACT AATCCCTTCC TGTGCGAATC AACGGAGCAG 
ATACCCAGTA ATCCCATTTC TGAACCTCTG AATGCCACGG TGGCTTCTCT GGAAAAAACG 
ACTATTTCAA CAAGTGAATG TACTCCTATA ACTGCTCCTC TAGAGACTCC AAATGGGCCT 
ATTTCAACGG ATACTCCATC CACCATACAT CACAATGGCA ATCCCTTCGT GGAGAGAGAA 
AGTCTGTCCA GCAGACTCCT GAAGATCCAT AACACAACGA ATCCTTTCAC GGGACTCTCG 
CAACGCGTCA AGGGTCCGCA TCTACTGCAA AAGACCATTT CGGAGGATTA TCTATTTCGG 
AAATTGGGTG TCAATTGTCC GATGGCCAAT GGCAATGGTA CGGGACCAGG ATCTGGTCAT 
GTCAACGGAA ATGGCAATGG AACTTGGTCC TTTGGACGCT CCCTGTTGCG ACAGGACTCC 
ACATTGAGTC TGGGCATGGG CTTGGGCAGA AGGAACAGCT CCCAGGTATC CCTGGATTCA 
CATGCGGGAT CGGTGCCCGG TTCAATGGAT GGCATCAATT TGGAGCGAGC CATCTCCTGC 
GATTCGGTAA CCTCGGACTC CACTCTATTC CTGGATCAAT TGGATCAGCC CTACACTCAG 
ATAACCGGAT ATTTATGCGT TGGCCTTAAC TACGATCAGA TGAGTATTAG CAACGAGGGC 
ATGGAACTGA CTGTTAGTGT CCTGGAGGCC AAGGGACTCA TATGTCCATT CAGCGTTGAA 
TCCCTGGACA CCTTTGTGCG CATCTACTTG GTGCCGGATC ATCCGGGAGC TATGCAAACT 
AAGGTGGTCA AGGGAACACT GACACCCAGC TACAACGAAA GTTTCGATTT CTGGCTGCAC 
AAGCGACAGG CGCGCCATTC GCTGTGGTTC CATCTGTACC ACAATGGACC CGCCCACACG 
CTCATTGGCG AGGCGGAGAT GGAAATTGGT GAGATGCCAC GCCCCATTAC CACATGGATA 
CCACTGTCCG ATTCGCGCAA GTGCAACGCC CGCTGGGGCG AGCTAATGTT CTCTCTAAGC 
TATCTGCCCA CGGCGGAAAG ACTAACCATC GTCGTGGTCA AGGCACGGAA TCTCAAGCTG 
GACGGTGAGC AACCGGCTCC GGAATCCGCG GAAACGGTGC ACAGCGTCTT CGTTAAGGTC 
TACCTGATGG ACAAGGATCG CAAGGTGCTA AAGAAGCGCA CCTCACTGAA GCGCAAGGAT 
CGCAGTCCGA TCTTCAACGA ATCGATGATC TTCAGTGTGC CGCCACCCAG TTTGACCACC 
ACCCAGCTGC GGGTGACCGT GTTCGGGGTG ACAACTAGTG GGGTGACCCC GTTGGGTCAC 
ATCGTGGCCG GCAGCTGTGC GGTGGGCAAG GGATTGCGCC ATTGGCACCA GATGCTCTCG 
TCGCTTAGGA AACCGGTGGC CATGTGGCAT GTCCTGCGGC GTGCGGTCAA TCAGCCGATT 
TTCACGGGCG GCGCCGAGGC GGTGGTGGCC GCCATGCAAA ACACCCTGCA ACGCTCCACC 
GCCAAGCGGA ACAGCATCGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Syt12 ( NM_001272584.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259513.1 Drosophila melanogaster synaptotagmin 12, transcript variant B (Syt12), mRNA.

Price & Availability↑

CloneID	OFa01488
Clone ID Related Accession (Same CDS sequence)	NM_132650.2 , NM_001272584.1
Accession Version	NM_001272584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	synaptotagmin 12, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCT TCACCATTAC CTTGGCGCTA CTGCTCCTGG CAGTTCTGAT CCTGGGCATC 
TTGTTCGCCT GCCACTGTCT TGGTCCGCGG GCCGCCAACT GGATGCGTAT GCGATCCAGC 
AAGGAGGAAA AGATCGGACT ATCCAATCAC AAGCAAAAGC TCTTCCATGC CAATGGTTAT 
ATGGCCAGTA TACAATCCGG CTCCGAGTTC CTGCTGAGCA CCAGTGGTAG TTTCAAGCGT 
TTCGATACCA TCGACAAGGA GGACTACAAT CGCTCCCAGC AGACGCATCA GACGCATCTG 
TTCCTGATGA ACGGCGGCGG TGCGGTTTCG ATAACCGGAT CATCCAGTGC CCTGCAGGCC 
ACCACGGCCA TGTGGCATCT ACCGCGTTCG GGTATTAAGA TTCCACCACC GCCCGATCGA 
CCAGCTCCAT CTCCGAATAG TGCTGCAGCA GCCGGCAAAA CGGTTACCTT CTCTTTGAGC 
CAGGTAAACG AGGTCATCAT GCCCGGCGAG CAGGTGACAT CCAACGATCA GGAACGTCGC 
TCCTGCCTGC GACAAAATGG TGGAACTGCG GGCGGTGCTG GCGGCGGTGG TGTGCTGCCG 
CAGCCACTGG AGGCACCACC TCCGCCACGA CTCACCGCAT CGACGTCGGT GAACTTATCG 
GGTACGGCGA TTCCAAGGCC CATTGCCAAG CGGCAAGTGT CCACGCAGCC GGCCAACAAT 
AGCGGATGCC AGCCGGAGCA GACCGTGGTG ACCCCGATGC GGGAGGGTGA GAGAAAAGCG 
CCTGCCTTGA AGCGTAGAAA CTCCAGCACT AATCCCTTCC TGTGCGAATC AACGGAGCAG 
ATACCCAGTA ATCCCATTTC TGAACCTCTG AATGCCACGG TGGCTTCTCT GGAAAAAACG 
ACTATTTCAA CAAGTGAATG TACTCCTATA ACTGCTCCTC TAGAGACTCC AAATGGGCCT 
ATTTCAACGG ATACTCCATC CACCATACAT CACAATGGCA ATCCCTTCGT GGAGAGAGAA 
AGTCTGTCCA GCAGACTCCT GAAGATCCAT AACACAACGA ATCCTTTCAC GGGACTCTCG 
CAACGCGTCA AGGGTCCGCA TCTACTGCAA AAGACCATTT CGGAGGATTA TCTATTTCGG 
AAATTGGGTG TCAATTGTCC GATGGCCAAT GGCAATGGTA CGGGACCAGG ATCTGGTCAT 
GTCAACGGAA ATGGCAATGG AACTTGGTCC TTTGGACGCT CCCTGTTGCG ACAGGACTCC 
ACATTGAGTC TGGGCATGGG CTTGGGCAGA AGGAACAGCT CCCAGGTATC CCTGGATTCA 
CATGCGGGAT CGGTGCCCGG TTCAATGGAT GGCATCAATT TGGAGCGAGC CATCTCCTGC 
GATTCGGTAA CCTCGGACTC CACTCTATTC CTGGATCAAT TGGATCAGCC CTACACTCAG 
ATAACCGGAT ATTTATGCGT TGGCCTTAAC TACGATCAGA TGAGTATTAG CAACGAGGGC 
ATGGAACTGA CTGTTAGTGT CCTGGAGGCC AAGGGACTCA TATGTCCATT CAGCGTTGAA 
TCCCTGGACA CCTTTGTGCG CATCTACTTG GTGCCGGATC ATCCGGGAGC TATGCAAACT 
AAGGTGGTCA AGGGAACACT GACACCCAGC TACAACGAAA GTTTCGATTT CTGGCTGCAC 
AAGCGACAGG CGCGCCATTC GCTGTGGTTC CATCTGTACC ACAATGGACC CGCCCACACG 
CTCATTGGCG AGGCGGAGAT GGAAATTGGT GAGATGCCAC GCCCCATTAC CACATGGATA 
CCACTGTCCG ATTCGCGCAA GTGCAACGCC CGCTGGGGCG AGCTAATGTT CTCTCTAAGC 
TATCTGCCCA CGGCGGAAAG ACTAACCATC GTCGTGGTCA AGGCACGGAA TCTCAAGCTG 
GACGGTGAGC AACCGGCTCC GGAATCCGCG GAAACGGTGC ACAGCGTCTT CGTTAAGGTC 
TACCTGATGG ACAAGGATCG CAAGGTGCTA AAGAAGCGCA CCTCACTGAA GCGCAAGGAT 
CGCAGTCCGA TCTTCAACGA ATCGATGATC TTCAGTGTGC CGCCACCCAG TTTGACCACC 
ACCCAGCTGC GGGTGACCGT GTTCGGGGTG ACAACTAGTG GGGTGACCCC GTTGGGTCAC 
ATCGTGGCCG GCAGCTGTGC GGTGGGCAAG GGATTGCGCC ATTGGCACCA GATGCTCTCG 
TCGCTTAGGA AACCGGTGGC CATGTGGCAT GTCCTGCGGC GTGCGGTCAA TCAGCCGATT 
TTCACGGGCG GCGCCGAGGC GGTGGTGGCC GCCATGCAAA ACACCCTGCA ACGCTCCACC 
GCCAAGCGGA ACAGCATCGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259513.1
CDS	239..2602
Translation	MMSFTITLALLLLAVLILGILFACHCLGPRAANWMRMRSSKEEKIGLSNHKQKLFHANGYMASIQSGSEFLLSTSGSFKRFDTIDKEDYNRSQQTHQTHLFLMNGGGAVSITGSSSALQATTAMWHLPRSGIKIPPPPDRPAPSPNSAAAAGKTVTFSLSQVNEVIMPGEQVTSNDQERRSCLRQNGGTAGGAGGGGVLPQPLEAPPPPRLTASTSVNLSGTAIPRPIAKRQVSTQPANNSGCQPEQTVVTPMREGERKAPALKRRNSSTNPFLCESTEQIPSNPISEPLNATVASLEKTTISTSECTPITAPLETPNGPISTDTPSTIHHNGNPFVERESLSSRLLKIHNTTNPFTGLSQRVKGPHLLQKTISEDYLFRKLGVNCPMANGNGTGPGSGHVNGNGNGTWSFGRSLLRQDSTLSLGMGLGRRNSSQVSLDSHAGSVPGSMDGINLERAISCDSVTSDSTLFLDQLDQPYTQITGYLCVGLNYDQMSISNEGMELTVSVLEAKGLICPFSVESLDTFVRIYLVPDHPGAMQTKVVKGTLTPSYNESFDFWLHKRQARHSLWFHLYHNGPAHTLIGEAEMEIGEMPRPITTWIPLSDSRKCNARWGELMFSLSYLPTAERLTIVVVKARNLKLDGEQPAPESAETVHSVFVKVYLMDKDRKVLKKRTSLKRKDRSPIFNESMIFSVPPPSLTTTQLRVTVFGVTTSGVTPLGHIVAGSCAVGKGLRHWHQMLSSLRKPVAMWHVLRRAVNQPIFTGGAEAVVAAMQNTLQRSTAKRNSIV

Target ORF information:

RefSeq Version	NM_001272584.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster synaptotagmin 12, transcript variant B (Syt12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272584.1

ORF Insert Sequence:

ATGATGAGCT TCACCATTAC CTTGGCGCTA CTGCTCCTGG CAGTTCTGAT CCTGGGCATC 
TTGTTCGCCT GCCACTGTCT TGGTCCGCGG GCCGCCAACT GGATGCGTAT GCGATCCAGC 
AAGGAGGAAA AGATCGGACT ATCCAATCAC AAGCAAAAGC TCTTCCATGC CAATGGTTAT 
ATGGCCAGTA TACAATCCGG CTCCGAGTTC CTGCTGAGCA CCAGTGGTAG TTTCAAGCGT 
TTCGATACCA TCGACAAGGA GGACTACAAT CGCTCCCAGC AGACGCATCA GACGCATCTG 
TTCCTGATGA ACGGCGGCGG TGCGGTTTCG ATAACCGGAT CATCCAGTGC CCTGCAGGCC 
ACCACGGCCA TGTGGCATCT ACCGCGTTCG GGTATTAAGA TTCCACCACC GCCCGATCGA 
CCAGCTCCAT CTCCGAATAG TGCTGCAGCA GCCGGCAAAA CGGTTACCTT CTCTTTGAGC 
CAGGTAAACG AGGTCATCAT GCCCGGCGAG CAGGTGACAT CCAACGATCA GGAACGTCGC 
TCCTGCCTGC GACAAAATGG TGGAACTGCG GGCGGTGCTG GCGGCGGTGG TGTGCTGCCG 
CAGCCACTGG AGGCACCACC TCCGCCACGA CTCACCGCAT CGACGTCGGT GAACTTATCG 
GGTACGGCGA TTCCAAGGCC CATTGCCAAG CGGCAAGTGT CCACGCAGCC GGCCAACAAT 
AGCGGATGCC AGCCGGAGCA GACCGTGGTG ACCCCGATGC GGGAGGGTGA GAGAAAAGCG 
CCTGCCTTGA AGCGTAGAAA CTCCAGCACT AATCCCTTCC TGTGCGAATC AACGGAGCAG 
ATACCCAGTA ATCCCATTTC TGAACCTCTG AATGCCACGG TGGCTTCTCT GGAAAAAACG 
ACTATTTCAA CAAGTGAATG TACTCCTATA ACTGCTCCTC TAGAGACTCC AAATGGGCCT 
ATTTCAACGG ATACTCCATC CACCATACAT CACAATGGCA ATCCCTTCGT GGAGAGAGAA 
AGTCTGTCCA GCAGACTCCT GAAGATCCAT AACACAACGA ATCCTTTCAC GGGACTCTCG 
CAACGCGTCA AGGGTCCGCA TCTACTGCAA AAGACCATTT CGGAGGATTA TCTATTTCGG 
AAATTGGGTG TCAATTGTCC GATGGCCAAT GGCAATGGTA CGGGACCAGG ATCTGGTCAT 
GTCAACGGAA ATGGCAATGG AACTTGGTCC TTTGGACGCT CCCTGTTGCG ACAGGACTCC 
ACATTGAGTC TGGGCATGGG CTTGGGCAGA AGGAACAGCT CCCAGGTATC CCTGGATTCA 
CATGCGGGAT CGGTGCCCGG TTCAATGGAT GGCATCAATT TGGAGCGAGC CATCTCCTGC 
GATTCGGTAA CCTCGGACTC CACTCTATTC CTGGATCAAT TGGATCAGCC CTACACTCAG 
ATAACCGGAT ATTTATGCGT TGGCCTTAAC TACGATCAGA TGAGTATTAG CAACGAGGGC 
ATGGAACTGA CTGTTAGTGT CCTGGAGGCC AAGGGACTCA TATGTCCATT CAGCGTTGAA 
TCCCTGGACA CCTTTGTGCG CATCTACTTG GTGCCGGATC ATCCGGGAGC TATGCAAACT 
AAGGTGGTCA AGGGAACACT GACACCCAGC TACAACGAAA GTTTCGATTT CTGGCTGCAC 
AAGCGACAGG CGCGCCATTC GCTGTGGTTC CATCTGTACC ACAATGGACC CGCCCACACG 
CTCATTGGCG AGGCGGAGAT GGAAATTGGT GAGATGCCAC GCCCCATTAC CACATGGATA 
CCACTGTCCG ATTCGCGCAA GTGCAACGCC CGCTGGGGCG AGCTAATGTT CTCTCTAAGC 
TATCTGCCCA CGGCGGAAAG ACTAACCATC GTCGTGGTCA AGGCACGGAA TCTCAAGCTG 
GACGGTGAGC AACCGGCTCC GGAATCCGCG GAAACGGTGC ACAGCGTCTT CGTTAAGGTC 
TACCTGATGG ACAAGGATCG CAAGGTGCTA AAGAAGCGCA CCTCACTGAA GCGCAAGGAT 
CGCAGTCCGA TCTTCAACGA ATCGATGATC TTCAGTGTGC CGCCACCCAG TTTGACCACC 
ACCCAGCTGC GGGTGACCGT GTTCGGGGTG ACAACTAGTG GGGTGACCCC GTTGGGTCAC 
ATCGTGGCCG GCAGCTGTGC GGTGGGCAAG GGATTGCGCC ATTGGCACCA GATGCTCTCG 
TCGCTTAGGA AACCGGTGGC CATGTGGCAT GTCCTGCGGC GTGCGGTCAA TCAGCCGATT 
TTCACGGGCG GCGCCGAGGC GGTGGTGGCC GCCATGCAAA ACACCCTGCA ACGCTCCACC 
GCCAAGCGGA ACAGCATCGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
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Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Syt12 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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