ND-75 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ND-75
Entrez Gene ID	31762
Full Name	NADH dehydrogenase (ubiquinone) 75 kDa subunit
Synonyms	CG2286,Dmel\CG2286,ND75,NDUFS1,NUAM
General protein information	Preferred Names NADH dehydrogenase (ubiquinone) 75 kDa subunit Names CG2286 gene product from transcript CG2286-RA CG2286-PA CG2286-PB FBpp0071128 NADH ubiquinone oxidoreductase 75 kDa NADH-ubiquinone oxidoreductase 75 kDa subunit NADH:ubiquinone oxidoreductase 75 kDa subunit NADH:ubiquinone reductase 75 kDa subunit NADH:ubiquinone reductase 75-kD subunit precursor NADH:ubiquinone reductase 75kD subunit precursor ND-75-PA ND-75-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7E1-7E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_167152.2	NP_727255.1	NADH dehydrogenase (ubiquinone) 75 kDa subunit, isoform B
NM_078528.3	NP_511083.1	NADH dehydrogenase (ubiquinone) 75 kDa subunit, isoform A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ndufs1	NP_001007766.1
Drosophila melanogaster (fruit fly)	ND75	NP_511083.1
Arabidopsis thaliana (thale cress)	EMB1467	NP_568550.1
Oryza sativa (rice)	Os03g0713400	NP_001051072.1
Xenopus tropicalis (tropical clawed frog)	ndufs1	XP_002936432.2
Neurospora crassa	NCU01765	XP_957188.2
Homo sapiens (human)	NDUFS1	NP_001186913.1
Macaca mulatta (Rhesus monkey)	NDUFS1	XP_001105816.2
Rattus norvegicus (Norway rat)	Ndufs1	NP_001005550.1
Caenorhabditis elegans (roundworm)	nuo-5	NP_503733.1
Canis lupus familiaris (dog)	NDUFS1	XP_005640630.1
Magnaporthe oryzae (rice blast fungus)	MGG_03155	XP_003716838.1
Pan troglodytes (chimpanzee)	NDUFS1	NP_001073384.1
Bos taurus (cattle)	NDUFS1	NP_777245.1
Mus musculus (house mouse)	Ndufs1	NP_001153510.1
Gallus gallus (chicken)	NDUFS1	NP_001006518.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001653	XP_003435895.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ND-75 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ND-75 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01101	NM_078528.3 Latest version!	Drosophila melanogaster NADH dehydrogenase (ubiquinone) 75 kDa subunit (ND-75), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OFa01101	NM_167152.2 Latest version!	Drosophila melanogaster NADH dehydrogenase (ubiquinone) 75 kDa subunit (ND-75), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ND-75 ( NM_167152.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727255.1 Drosophila melanogaster NADH dehydrogenase (ubiquinone) 75 kDa subunit (ND-75), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01101
Clone ID Related Accession (Same CDS sequence)	NM_167152.2 , NM_078528.3
Accession Version	NM_167152.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	NADH dehydrogenase (ubiquinone) 75 kDa subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_167152.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATACGGG CACCGCTAGT CAAGGCGCTG GGCGCTCTGG GCTCGCCCAC ACATCAGATG 
GCCAGCCGGG CGGTGCGAAC CAGCGCCATG GTGGCCCAGA CACCGGCGAA AGCGCCCGAG 
AAGATCGAGG TCTTTGTGGA TGACATTCCC GTGCAGGTGG TACCCGGCAC CACTGTCCTC 
CAGGCTGCTG CTCAGATCGG CGTGGAGATA CCCAGATTCT GCTACCACGA ACGTCTTGCG 
GTGGCCGGCA ACTGCAGGAT GTGCCTCGTC GAGGTGGAGA AGAGTCCCAA GCCAGTGGCC 
GCCTGCGCTA TGCCCGTGAT GAAGGGCTGG CGCATCAAGA CCAACTCGGA TTTGACCCGC 
AAGGCGCGCG AGGGCGTCAT GGAGTTCCTG CTGATGAACC ATCCGCTGGA TTGCCCCATC 
TGCGATCAGG GCGGCGAGTG CGATCTGCAG GATCAGGCCA TGGCCTTCGG TTCCGATCGA 
TCCCGCTTCA CGGACATTAA CTACACGGGC AAGCGCGCTG TCGAGGACAA GGACATCGGA 
CCGCTAGTCA AGACCATCAT GACACGCTGC ATCCACTGCA CCCGCTGTGT GCGTTTCGCC 
TCCGAGATTG CCGGCGTTGA TGACCTGGGC ACCACTGGCC GTGGCAACGA CATGCAGATC 
GGCACCTACG TGGAGAAGCT CTTCCTTACC GAACTGTCCG GCAACGTGAT CGACCTGTGC 
CCCGTGGGAG CGCTGACCAA CAAGCCCTAC AGTTTTGTGG CCCGTCCCTG GGAGATCCGT 
AAGGTGAGCA GCATCGATGT GCTGGACGCC GTCGGCAGCA ACATTGTCGT CAGCACCCGC 
ACCAATGAGG TGCTGCGCAT CCTGCCCCGT GAGAACGAGG ACGTCAACGA GGAATGGCTT 
GCTGACAAAT CGCGCTTCGC CTGCGACGGT CTGAAGCGCC AGCGCCTGGT GGCTCCCATG 
GTGCGCATGC CCAACGGCGA ACTACAGGCC GTAGAGTGGG AGGGTGCCCT CATCGCCGTG 
GCCAAGGCCA TTAAGGCAGC CGGTGGACAG ATTGCCGGTA TTTCTGGCCA ACTTGCCGAC 
CTGGAGGCTC AGGTGGCCCT GAAGGATCTG CTGAACCGTC TGGGCAGCGA AGTGGTCGCC 
ACTGAACAAG GATTTATTGC CGGAGGCACC GACAACCGTG CTAATTACCT CTTGAACAGC 
ACCATTGCCG GTTTGGAGGA GGCCGACGCC GTTCTCCTGG TGGGCACCAA TCCCCGTTAC 
GAGGCTCCAT TGGTCAACAC CCGTTTGCGC AAGGCCTATG TGCACAACGA ACTGCAGATT 
GCCTCGATTG GACCCAAGAT CGATCTGTCC TACGACCACG AGAACCTTGG CGCCGATGCC 
GCTTTAGTCA AGGACGTGTG CTCCGGAGCT CATGCCTTCT CCAAGGTCTT GGAGGGTGCC 
AAGAAGCCGG CCATCATTAT TGGAGCCGAT TTGTTGGAGC GTGCCGATGG TGCCGCCATT 
CACGCCACCG TTGCCGAGTA CTGCAAGAAG CTGAAGAAGC CGAACTGGAA CCCCTTCAAT 
GTGCTGCAGA CCAACGCCGC CCAGGTGGGC GCCCTCGATG TGGGCTACAA GGCCGGTGCA 
CAGACCGCTG TGAAGGCTCA GCCCAAGGTG CTGTTCCTGT TGAACGCCGA CGCTGGCAAG 
GTGACTCGCG AGCAGCTGCC CAAGGACTGC TTCGTGGTGT ACATTGGATC GCATGGTGAC 
AACGGCGCCT CCATTGCCGA TGCCGTTCTG CCTGGTGCCG CCTACACAGA GAAGCAGGGC 
ATCTACGTGA ACACGGAGGG CAGGCCGCAG CAGACGCTGC CTGGTGTTTC GCCACCGGGT 
ATGGCTCGCG AGGACTGGAA GATCCTGCGC GCTCTGTCCG AGGTGGTGGG CAAGCCGCTG 
CCGTACGACA ACCTGGACGA GCTGCGCAAC CGTCTGGAGG ATGTGGCGCC ACATCTAACG 
CGCCTGGGCC AATTGGAGCC AGCCGGCGAT GCCGGAGCAG CGGGCACTAT TAGCAAATCC 
ATCGGCGGTG GCGCCATCGA TATTAAGCTA AAGGAGCTGC GCGACTACTT CATGACCGAC 
GCCATTTCGC GCGCCTCGCC CACCATGGCC AAGTGCATAT CGGCGGTCAA CAAGCAGCAG 
CGGGAGAACG AGGCCAAGCA GAGTGTGGCT ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727255.1
CDS	69..2264
Translation	MIRAPLVKALGALGSPTHQMASRAVRTSAMVAQTPAKAPEKIEVFVDDIPVQVVPGTTVLQAAAQIGVEIPRFCYHERLAVAGNCRMCLVEVEKSPKPVAACAMPVMKGWRIKTNSDLTRKAREGVMEFLLMNHPLDCPICDQGGECDLQDQAMAFGSDRSRFTDINYTGKRAVEDKDIGPLVKTIMTRCIHCTRCVRFASEIAGVDDLGTTGRGNDMQIGTYVEKLFLTELSGNVIDLCPVGALTNKPYSFVARPWEIRKVSSIDVLDAVGSNIVVSTRTNEVLRILPRENEDVNEEWLADKSRFACDGLKRQRLVAPMVRMPNGELQAVEWEGALIAVAKAIKAAGGQIAGISGQLADLEAQVALKDLLNRLGSEVVATEQGFIAGGTDNRANYLLNSTIAGLEEADAVLLVGTNPRYEAPLVNTRLRKAYVHNELQIASIGPKIDLSYDHENLGADAALVKDVCSGAHAFSKVLEGAKKPAIIIGADLLERADGAAIHATVAEYCKKLKKPNWNPFNVLQTNAAQVGALDVGYKAGAQTAVKAQPKVLFLLNADAGKVTREQLPKDCFVVYIGSHGDNGASIADAVLPGAAYTEKQGIYVNTEGRPQQTLPGVSPPGMAREDWKILRALSEVVGKPLPYDNLDELRNRLEDVAPHLTRLGQLEPAGDAGAAGTISKSIGGGAIDIKLKELRDYFMTDAISRASPTMAKCISAVNKQQRENEAKQSVAI

Target ORF information:

RefSeq Version	NM_167152.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NADH dehydrogenase (ubiquinone) 75 kDa subunit (ND-75), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167152.2

ORF Insert Sequence:

ATGATACGGG CACCGCTAGT CAAGGCGCTG GGCGCTCTGG GCTCGCCCAC ACATCAGATG 
GCCAGCCGGG CGGTGCGAAC CAGCGCCATG GTGGCCCAGA CACCGGCGAA AGCGCCCGAG 
AAGATCGAGG TCTTTGTGGA TGACATTCCC GTGCAGGTGG TACCCGGCAC CACTGTCCTC 
CAGGCTGCTG CTCAGATCGG CGTGGAGATA CCCAGATTCT GCTACCACGA ACGTCTTGCG 
GTGGCCGGCA ACTGCAGGAT GTGCCTCGTC GAGGTGGAGA AGAGTCCCAA GCCAGTGGCC 
GCCTGCGCTA TGCCCGTGAT GAAGGGCTGG CGCATCAAGA CCAACTCGGA TTTGACCCGC 
AAGGCGCGCG AGGGCGTCAT GGAGTTCCTG CTGATGAACC ATCCGCTGGA TTGCCCCATC 
TGCGATCAGG GCGGCGAGTG CGATCTGCAG GATCAGGCCA TGGCCTTCGG TTCCGATCGA 
TCCCGCTTCA CGGACATTAA CTACACGGGC AAGCGCGCTG TCGAGGACAA GGACATCGGA 
CCGCTAGTCA AGACCATCAT GACACGCTGC ATCCACTGCA CCCGCTGTGT GCGTTTCGCC 
TCCGAGATTG CCGGCGTTGA TGACCTGGGC ACCACTGGCC GTGGCAACGA CATGCAGATC 
GGCACCTACG TGGAGAAGCT CTTCCTTACC GAACTGTCCG GCAACGTGAT CGACCTGTGC 
CCCGTGGGAG CGCTGACCAA CAAGCCCTAC AGTTTTGTGG CCCGTCCCTG GGAGATCCGT 
AAGGTGAGCA GCATCGATGT GCTGGACGCC GTCGGCAGCA ACATTGTCGT CAGCACCCGC 
ACCAATGAGG TGCTGCGCAT CCTGCCCCGT GAGAACGAGG ACGTCAACGA GGAATGGCTT 
GCTGACAAAT CGCGCTTCGC CTGCGACGGT CTGAAGCGCC AGCGCCTGGT GGCTCCCATG 
GTGCGCATGC CCAACGGCGA ACTACAGGCC GTAGAGTGGG AGGGTGCCCT CATCGCCGTG 
GCCAAGGCCA TTAAGGCAGC CGGTGGACAG ATTGCCGGTA TTTCTGGCCA ACTTGCCGAC 
CTGGAGGCTC AGGTGGCCCT GAAGGATCTG CTGAACCGTC TGGGCAGCGA AGTGGTCGCC 
ACTGAACAAG GATTTATTGC CGGAGGCACC GACAACCGTG CTAATTACCT CTTGAACAGC 
ACCATTGCCG GTTTGGAGGA GGCCGACGCC GTTCTCCTGG TGGGCACCAA TCCCCGTTAC 
GAGGCTCCAT TGGTCAACAC CCGTTTGCGC AAGGCCTATG TGCACAACGA ACTGCAGATT 
GCCTCGATTG GACCCAAGAT CGATCTGTCC TACGACCACG AGAACCTTGG CGCCGATGCC 
GCTTTAGTCA AGGACGTGTG CTCCGGAGCT CATGCCTTCT CCAAGGTCTT GGAGGGTGCC 
AAGAAGCCGG CCATCATTAT TGGAGCCGAT TTGTTGGAGC GTGCCGATGG TGCCGCCATT 
CACGCCACCG TTGCCGAGTA CTGCAAGAAG CTGAAGAAGC CGAACTGGAA CCCCTTCAAT 
GTGCTGCAGA CCAACGCCGC CCAGGTGGGC GCCCTCGATG TGGGCTACAA GGCCGGTGCA 
CAGACCGCTG TGAAGGCTCA GCCCAAGGTG CTGTTCCTGT TGAACGCCGA CGCTGGCAAG 
GTGACTCGCG AGCAGCTGCC CAAGGACTGC TTCGTGGTGT ACATTGGATC GCATGGTGAC 
AACGGCGCCT CCATTGCCGA TGCCGTTCTG CCTGGTGCCG CCTACACAGA GAAGCAGGGC 
ATCTACGTGA ACACGGAGGG CAGGCCGCAG CAGACGCTGC CTGGTGTTTC GCCACCGGGT 
ATGGCTCGCG AGGACTGGAA GATCCTGCGC GCTCTGTCCG AGGTGGTGGG CAAGCCGCTG 
CCGTACGACA ACCTGGACGA GCTGCGCAAC CGTCTGGAGG ATGTGGCGCC ACATCTAACG 
CGCCTGGGCC AATTGGAGCC AGCCGGCGAT GCCGGAGCAG CGGGCACTAT TAGCAAATCC 
ATCGGCGGTG GCGCCATCGA TATTAAGCTA AAGGAGCTGC GCGACTACTT CATGACCGAC 
GCCATTTCGC GCGCCTCGCC CACCATGGCC AAGTGCATAT CGGCGGTCAA CAAGCAGCAG 
CGGGAGAACG AGGCCAAGCA GAGTGTGGCT ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ND-75 ( NM_078528.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511083.1 Drosophila melanogaster NADH dehydrogenase (ubiquinone) 75 kDa subunit (ND-75), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01101
Clone ID Related Accession (Same CDS sequence)	NM_167152.2 , NM_078528.3
Accession Version	NM_078528.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	NADH dehydrogenase (ubiquinone) 75 kDa subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078528.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATACGGG CACCGCTAGT CAAGGCGCTG GGCGCTCTGG GCTCGCCCAC ACATCAGATG 
GCCAGCCGGG CGGTGCGAAC CAGCGCCATG GTGGCCCAGA CACCGGCGAA AGCGCCCGAG 
AAGATCGAGG TCTTTGTGGA TGACATTCCC GTGCAGGTGG TACCCGGCAC CACTGTCCTC 
CAGGCTGCTG CTCAGATCGG CGTGGAGATA CCCAGATTCT GCTACCACGA ACGTCTTGCG 
GTGGCCGGCA ACTGCAGGAT GTGCCTCGTC GAGGTGGAGA AGAGTCCCAA GCCAGTGGCC 
GCCTGCGCTA TGCCCGTGAT GAAGGGCTGG CGCATCAAGA CCAACTCGGA TTTGACCCGC 
AAGGCGCGCG AGGGCGTCAT GGAGTTCCTG CTGATGAACC ATCCGCTGGA TTGCCCCATC 
TGCGATCAGG GCGGCGAGTG CGATCTGCAG GATCAGGCCA TGGCCTTCGG TTCCGATCGA 
TCCCGCTTCA CGGACATTAA CTACACGGGC AAGCGCGCTG TCGAGGACAA GGACATCGGA 
CCGCTAGTCA AGACCATCAT GACACGCTGC ATCCACTGCA CCCGCTGTGT GCGTTTCGCC 
TCCGAGATTG CCGGCGTTGA TGACCTGGGC ACCACTGGCC GTGGCAACGA CATGCAGATC 
GGCACCTACG TGGAGAAGCT CTTCCTTACC GAACTGTCCG GCAACGTGAT CGACCTGTGC 
CCCGTGGGAG CGCTGACCAA CAAGCCCTAC AGTTTTGTGG CCCGTCCCTG GGAGATCCGT 
AAGGTGAGCA GCATCGATGT GCTGGACGCC GTCGGCAGCA ACATTGTCGT CAGCACCCGC 
ACCAATGAGG TGCTGCGCAT CCTGCCCCGT GAGAACGAGG ACGTCAACGA GGAATGGCTT 
GCTGACAAAT CGCGCTTCGC CTGCGACGGT CTGAAGCGCC AGCGCCTGGT GGCTCCCATG 
GTGCGCATGC CCAACGGCGA ACTACAGGCC GTAGAGTGGG AGGGTGCCCT CATCGCCGTG 
GCCAAGGCCA TTAAGGCAGC CGGTGGACAG ATTGCCGGTA TTTCTGGCCA ACTTGCCGAC 
CTGGAGGCTC AGGTGGCCCT GAAGGATCTG CTGAACCGTC TGGGCAGCGA AGTGGTCGCC 
ACTGAACAAG GATTTATTGC CGGAGGCACC GACAACCGTG CTAATTACCT CTTGAACAGC 
ACCATTGCCG GTTTGGAGGA GGCCGACGCC GTTCTCCTGG TGGGCACCAA TCCCCGTTAC 
GAGGCTCCAT TGGTCAACAC CCGTTTGCGC AAGGCCTATG TGCACAACGA ACTGCAGATT 
GCCTCGATTG GACCCAAGAT CGATCTGTCC TACGACCACG AGAACCTTGG CGCCGATGCC 
GCTTTAGTCA AGGACGTGTG CTCCGGAGCT CATGCCTTCT CCAAGGTCTT GGAGGGTGCC 
AAGAAGCCGG CCATCATTAT TGGAGCCGAT TTGTTGGAGC GTGCCGATGG TGCCGCCATT 
CACGCCACCG TTGCCGAGTA CTGCAAGAAG CTGAAGAAGC CGAACTGGAA CCCCTTCAAT 
GTGCTGCAGA CCAACGCCGC CCAGGTGGGC GCCCTCGATG TGGGCTACAA GGCCGGTGCA 
CAGACCGCTG TGAAGGCTCA GCCCAAGGTG CTGTTCCTGT TGAACGCCGA CGCTGGCAAG 
GTGACTCGCG AGCAGCTGCC CAAGGACTGC TTCGTGGTGT ACATTGGATC GCATGGTGAC 
AACGGCGCCT CCATTGCCGA TGCCGTTCTG CCTGGTGCCG CCTACACAGA GAAGCAGGGC 
ATCTACGTGA ACACGGAGGG CAGGCCGCAG CAGACGCTGC CTGGTGTTTC GCCACCGGGT 
ATGGCTCGCG AGGACTGGAA GATCCTGCGC GCTCTGTCCG AGGTGGTGGG CAAGCCGCTG 
CCGTACGACA ACCTGGACGA GCTGCGCAAC CGTCTGGAGG ATGTGGCGCC ACATCTAACG 
CGCCTGGGCC AATTGGAGCC AGCCGGCGAT GCCGGAGCAG CGGGCACTAT TAGCAAATCC 
ATCGGCGGTG GCGCCATCGA TATTAAGCTA AAGGAGCTGC GCGACTACTT CATGACCGAC 
GCCATTTCGC GCGCCTCGCC CACCATGGCC AAGTGCATAT CGGCGGTCAA CAAGCAGCAG 
CGGGAGAACG AGGCCAAGCA GAGTGTGGCT ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511083.1
CDS	97..2292
Translation	MIRAPLVKALGALGSPTHQMASRAVRTSAMVAQTPAKAPEKIEVFVDDIPVQVVPGTTVLQAAAQIGVEIPRFCYHERLAVAGNCRMCLVEVEKSPKPVAACAMPVMKGWRIKTNSDLTRKAREGVMEFLLMNHPLDCPICDQGGECDLQDQAMAFGSDRSRFTDINYTGKRAVEDKDIGPLVKTIMTRCIHCTRCVRFASEIAGVDDLGTTGRGNDMQIGTYVEKLFLTELSGNVIDLCPVGALTNKPYSFVARPWEIRKVSSIDVLDAVGSNIVVSTRTNEVLRILPRENEDVNEEWLADKSRFACDGLKRQRLVAPMVRMPNGELQAVEWEGALIAVAKAIKAAGGQIAGISGQLADLEAQVALKDLLNRLGSEVVATEQGFIAGGTDNRANYLLNSTIAGLEEADAVLLVGTNPRYEAPLVNTRLRKAYVHNELQIASIGPKIDLSYDHENLGADAALVKDVCSGAHAFSKVLEGAKKPAIIIGADLLERADGAAIHATVAEYCKKLKKPNWNPFNVLQTNAAQVGALDVGYKAGAQTAVKAQPKVLFLLNADAGKVTREQLPKDCFVVYIGSHGDNGASIADAVLPGAAYTEKQGIYVNTEGRPQQTLPGVSPPGMAREDWKILRALSEVVGKPLPYDNLDELRNRLEDVAPHLTRLGQLEPAGDAGAAGTISKSIGGGAIDIKLKELRDYFMTDAISRASPTMAKCISAVNKQQRENEAKQSVAI

Target ORF information:

RefSeq Version	NM_078528.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NADH dehydrogenase (ubiquinone) 75 kDa subunit (ND-75), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078528.3

ORF Insert Sequence:

ATGATACGGG CACCGCTAGT CAAGGCGCTG GGCGCTCTGG GCTCGCCCAC ACATCAGATG 
GCCAGCCGGG CGGTGCGAAC CAGCGCCATG GTGGCCCAGA CACCGGCGAA AGCGCCCGAG 
AAGATCGAGG TCTTTGTGGA TGACATTCCC GTGCAGGTGG TACCCGGCAC CACTGTCCTC 
CAGGCTGCTG CTCAGATCGG CGTGGAGATA CCCAGATTCT GCTACCACGA ACGTCTTGCG 
GTGGCCGGCA ACTGCAGGAT GTGCCTCGTC GAGGTGGAGA AGAGTCCCAA GCCAGTGGCC 
GCCTGCGCTA TGCCCGTGAT GAAGGGCTGG CGCATCAAGA CCAACTCGGA TTTGACCCGC 
AAGGCGCGCG AGGGCGTCAT GGAGTTCCTG CTGATGAACC ATCCGCTGGA TTGCCCCATC 
TGCGATCAGG GCGGCGAGTG CGATCTGCAG GATCAGGCCA TGGCCTTCGG TTCCGATCGA 
TCCCGCTTCA CGGACATTAA CTACACGGGC AAGCGCGCTG TCGAGGACAA GGACATCGGA 
CCGCTAGTCA AGACCATCAT GACACGCTGC ATCCACTGCA CCCGCTGTGT GCGTTTCGCC 
TCCGAGATTG CCGGCGTTGA TGACCTGGGC ACCACTGGCC GTGGCAACGA CATGCAGATC 
GGCACCTACG TGGAGAAGCT CTTCCTTACC GAACTGTCCG GCAACGTGAT CGACCTGTGC 
CCCGTGGGAG CGCTGACCAA CAAGCCCTAC AGTTTTGTGG CCCGTCCCTG GGAGATCCGT 
AAGGTGAGCA GCATCGATGT GCTGGACGCC GTCGGCAGCA ACATTGTCGT CAGCACCCGC 
ACCAATGAGG TGCTGCGCAT CCTGCCCCGT GAGAACGAGG ACGTCAACGA GGAATGGCTT 
GCTGACAAAT CGCGCTTCGC CTGCGACGGT CTGAAGCGCC AGCGCCTGGT GGCTCCCATG 
GTGCGCATGC CCAACGGCGA ACTACAGGCC GTAGAGTGGG AGGGTGCCCT CATCGCCGTG 
GCCAAGGCCA TTAAGGCAGC CGGTGGACAG ATTGCCGGTA TTTCTGGCCA ACTTGCCGAC 
CTGGAGGCTC AGGTGGCCCT GAAGGATCTG CTGAACCGTC TGGGCAGCGA AGTGGTCGCC 
ACTGAACAAG GATTTATTGC CGGAGGCACC GACAACCGTG CTAATTACCT CTTGAACAGC 
ACCATTGCCG GTTTGGAGGA GGCCGACGCC GTTCTCCTGG TGGGCACCAA TCCCCGTTAC 
GAGGCTCCAT TGGTCAACAC CCGTTTGCGC AAGGCCTATG TGCACAACGA ACTGCAGATT 
GCCTCGATTG GACCCAAGAT CGATCTGTCC TACGACCACG AGAACCTTGG CGCCGATGCC 
GCTTTAGTCA AGGACGTGTG CTCCGGAGCT CATGCCTTCT CCAAGGTCTT GGAGGGTGCC 
AAGAAGCCGG CCATCATTAT TGGAGCCGAT TTGTTGGAGC GTGCCGATGG TGCCGCCATT 
CACGCCACCG TTGCCGAGTA CTGCAAGAAG CTGAAGAAGC CGAACTGGAA CCCCTTCAAT 
GTGCTGCAGA CCAACGCCGC CCAGGTGGGC GCCCTCGATG TGGGCTACAA GGCCGGTGCA 
CAGACCGCTG TGAAGGCTCA GCCCAAGGTG CTGTTCCTGT TGAACGCCGA CGCTGGCAAG 
GTGACTCGCG AGCAGCTGCC CAAGGACTGC TTCGTGGTGT ACATTGGATC GCATGGTGAC 
AACGGCGCCT CCATTGCCGA TGCCGTTCTG CCTGGTGCCG CCTACACAGA GAAGCAGGGC 
ATCTACGTGA ACACGGAGGG CAGGCCGCAG CAGACGCTGC CTGGTGTTTC GCCACCGGGT 
ATGGCTCGCG AGGACTGGAA GATCCTGCGC GCTCTGTCCG AGGTGGTGGG CAAGCCGCTG 
CCGTACGACA ACCTGGACGA GCTGCGCAAC CGTCTGGAGG ATGTGGCGCC ACATCTAACG 
CGCCTGGGCC AATTGGAGCC AGCCGGCGAT GCCGGAGCAG CGGGCACTAT TAGCAAATCC 
ATCGGCGGTG GCGCCATCGA TATTAAGCTA AAGGAGCTGC GCGACTACTT CATGACCGAC 
GCCATTTCGC GCGCCTCGCC CACCATGGCC AAGTGCATAT CGGCGGTCAA CAAGCAGCAG 
CGGGAGAACG AGGCCAAGCA GAGTGTGGCT ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ND-75 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

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ORF Insert Sequence:

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ORF Insert Sequence:

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