Dscam4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dscam4
Entrez Gene ID	2769008
Full Name	Down syndrome cell adhesion molecule 4
Synonyms	CG13672,CG18630,CG33274,CG42330,CG7060,CT21829,Dmel\CG42330,Dmel_CG13672,Dmel_CG33274
General protein information	Preferred Names Down syndrome cell adhesion molecule 4 Names CG42330 gene product from transcript CG42330-RD CG42330-PD CG42330-PE CG42330-PH CG42330-PI CG42330-PJ Dscam4-PD Dscam4-PE Dscam4-PH Dscam4-PI Dscam4-PJ down syndrome cell adhesion molecule 4
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66C1-66C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274643.2	NP_001261572.1	down syndrome cell adhesion molecule 4, isoform J
NM_001043131.3	NP_001036596.2	down syndrome cell adhesion molecule 4, isoform E
NM_001274642.1	NP_001261571.1	down syndrome cell adhesion molecule 4, isoform I
NM_001144437.2	NP_001137909.1	down syndrome cell adhesion molecule 4, isoform H
NM_001144436.2	NP_001137908.1	down syndrome cell adhesion molecule 4, isoform D

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DSCAM	XP_001171538.1
Gallus gallus (chicken)	DSCAM	XP_416734.4
Danio rerio (zebrafish)	dscama	NP_001025395.1
Canis lupus familiaris (dog)	DSCAM	XP_005639051.1
Mus musculus (house mouse)	Dscam	NP_112451.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007092	XP_308666.4
Bos taurus (cattle)	DSCAM	XP_002685157.1
Drosophila melanogaster (fruit fly)	Dscam4	NP_001137908.1
Homo sapiens (human)	DSCAM	NP_001380.2
Macaca mulatta (Rhesus monkey)	DSCAM	XP_002803170.1
Rattus norvegicus (Norway rat)	Dscam	NP_598271.1
Xenopus tropicalis (tropical clawed frog)	dscam	NP_001136135.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dscam4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dscam4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19637	NM_001274642.1 Latest version!	Drosophila melanogaster down syndrome cell adhesion molecule 4, transcript variant I (Dscam4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFa19637	NM_001274643.2 Latest version!	Drosophila melanogaster down syndrome cell adhesion molecule 4, transcript variant J (Dscam4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFa20093	NM_001144436.2 Latest version!	Drosophila melanogaster down syndrome cell adhesion molecule 4, transcript variant D (Dscam4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFa20093	NM_001043131.3 Latest version!	Drosophila melanogaster down syndrome cell adhesion molecule 4, transcript variant E (Dscam4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFa20094	NM_001144437.2 Latest version!	Drosophila melanogaster down syndrome cell adhesion molecule 4, transcript variant H (Dscam4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dscam4 ( NM_001274643.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261572.1 Drosophila melanogaster down syndrome cell adhesion molecule 4, transcript variant J (Dscam4), mRNA.

Price & Availability↑

CloneID	OFa19637
Clone ID Related Accession (Same CDS sequence)	NM_001274643.2 , NM_001274642.1
Accession Version	NM_001274643.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	down syndrome cell adhesion molecule 4, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001274643.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTTAC ACAGCCTTTT TAAGGCCATC CTGATCCTAT ATGTCATAAG AGCCGAGACC 
AGCCAGTTTG TAAATGGACT GGATCTACAG GGACCGATTT TTCTCCATGA GCCACCGCAT 
CGCGTCGAGT TCTCCAACAA TTCGGGCGGA CTCATAGAAT GCTCCGGGCA TGGAAGTCCC 
CCGCCGGAGG TGGAGTGGAC CCCCATCCCG CCGCAGCAGG ACATGGTGTT CCAGCTCTCC 
AACGGTAGCA TGATGTTCTA TCCCTTTACG GCCGAGAAGT ACCGCCACGA GGTGCATGCC 
ACAGTCTACA GATGCAAACT GCGCAATTTG GTGGGCACCG TGCTCAGCCG AGAGGTTCAC 
GTGCGCGGCG TCGTCAACCA GAAGTACGCG GTCCAGGTGC ACGATGAATA CGTGATGACG 
GGCAATACGG CGGTGCTGAA ATGCCAGGTT CCCAGCTACA TGTCGGAATT CGTATTGGTC 
ACCGCTTGGG TGCAGGACAC CGGCATGCAT TTGTATCCGA ACACGGACAT CGGTGGCAAG 
TACACTGTGC TATCGAACGG CGAATTGTAC ATAAATAATG CGGGACCCAA TGATGCGTAC 
AAAAGCTACA CATGCCGCAC TGTAAATAGA CTGACAGGTG AAGTACAAAT TTCCACATAT 
CCCGGCCGCA TTATTGTAAC AGAGCCCAAG GGCATGGTAC AGCCGCGCAT CAACGTGGAG 
AAGCATTCGA TGCGGCATGT TGTCCTTAAT GGCCAAACAA CGTTGCCGTG CATAGCTCAA 
GGACATCCGG TGCCAACATA TCGGTGGTTC AAGGAGGAAA ACGAGCAACT GCTGCCGCTG 
CAACTCAGCG AACGCATCAC CATCGTATCC GCAGGACTCC TCAAAATCAC CAAGGCACGT 
CTCGAGGATA GTGGAAAATA TTTGTGCTGG GTGAACAATA CGGCTGGCGA GGAGACCATC 
CAAGTGTCGC TAACGGTGAC AGCTCCTTTG ACGGCGCACC TACAGCCACA GGTGCAGACG 
GTGGATGTCG ATAAGGATGC GCAATTCCAA TGCATTGTCT CTGGCCATCC GGTCCACGAT 
GTCAACTGGC TGCACGACGG CAAACCCATA CTCAGGGACA ATCGGGTTGA GATACTCACC 
GATCCACCGC GACTGATAAT TAAAAAAGTG CAAAAGGAGG ACCCTGGCAT GTACCAGTGC 
TTCGTCTCCA ACGAATGGGA GCAGATCCAA TCCACCGCTG AACTGCAGTT GGGCGATGCC 
TCGCCGGAAC TGCTTTATTG GTTCTCGGAG CAAACGCTGC AGCCAGGACC AACGGTATCA 
TTGAAGTGCG TAGCCACCGG AAATCCACTG CCTCAATTTA CATGGTCCCT GGACGGATTT 
CCGATACCAG ACAGCTCGCG CTTTTTAGTG GGTCAATATG TTACCATCCA CGACGATGTC 
ATCAGCCACG TGAATATATC AAATGTCAAG GAGGAGGACG GCGGGGAGTA CACCTGCACG 
GCCCAGAATG CAATTGGCAA AGTCTCGCAC AGCGCCAAAG TGAACATCTA TGGATTGCCT 
TACATACGCG AAATGCCAAA AATAACAGGC ATTTCTGGCT CTGATTTGAT TGTTAAATGT 
CCCGTGGCTG GTTACCCCAT CGATAAAATT CACTGGGAGC GAGATGGCCA AACGCTGCCC 
ATAAATCGCA GGCAGCGTGC CTACAACAAT GGCACCTTAA TTATCGAGCA ACTGCAGCGC 
CTCGAGGATG CGGGCACCTA CACGTGCATG GCGCAGAACA AACAAAAGCA AACATCCCGC 
CGGAATGTGG AGATCCAAGT GCTGGTGCCG CCGAAAATTA TGCCCATCCA AGCCATGACG 
AACATGCTCC GCGAGGGAAT GCGCGCCGCC ATATCCTGCC AAATCCTCGA GGGCGACCTG 
CCCGTCAGTT TTCGATGGGA GCGCAACGGA AAGCCACTGA TCGGAACGGG CAATGAGGTT 
TTCCGCCGCC TGGATGAGTA TTCCGCCAGT TTGGTCATCG AGCACATTTC CTCCGACCAT 
TCCGGAAACT ACACCTGCAT CGCGAGCAAT GTGGCAGGTA CGGAAAGATT CACCGTACCC 
CTGACCGTGA ATGTACCGCC CAAGTGGATC CTGGAGCCAA AGGACTCGAG TGCCCAGGCG 
GGTGCAGATG TCCTTCTGCA CTGTCAATCC TCAGGATATC CCACACCGAC AATCACGTGG 
AAAAAGGCGA TTGGACCAAC GCCAGGGGAG TACAAGGATT TCCTTTACGA GCCCACAGTG 
CAGCTATTTC CCAACGGAAC CATTTTCTTC AAGAAGATCA GCAAGGAGTC GCAAGGACAC 
TTCCTGTGCG AGGCCAAAAA TAACATTGGT TCCGGTGTCA GCAAAGTCAT CTTCCTCAAA 
GTGAATGTGC CCGCCCACTT TCAGACGAAG ACCAAACAGA TTTCGGTGGC CAAAGGAAAG 
CAGGTCCATG TGCAGTGCAA CGTGCAGGGC GACAATCCCA TCGACTTTAA ATGGAAAATC 
CAGGCAACGC AACAGTATCT CGACGAGTCG CTGGATTCCC GCTACACAAT AAGAGATCAA 
GTGCTCGACG ACGGCATGGT CTCCGAATTG GGCATTTCGC ACACATATCG CCAGGATACG 
GGCATTTATA TCTGTCAGGC GAGCAATGCA TTCGGACAGG ACGAGATGTC CATTCAGCTC 
ATCGTGCAGG AAGTGCCCGA GCAGCCGAAG AACCTTCGCA TCAACTCGCA ACAGTCGCGC 
AGCCTTCAGC TCACGTGGAG CCAACCCTTT GCCGGAAATA GTCCCATCGA GGAGTACCAC 
ATCTACTACA AACAAATATC AGACATTTGG CAAAATGCGG AGCACCTCAC CATCGCCGGA 
GCCCAGACCG TGATCAACAT CCAGCAGCTG CGACCGGCAA AAGCCTATCA CATCCGCATG 
TCGGCGGAGA ACAAGCTGGG CGCCTCCGAG TTCTCGGAGG TGGTGCAAGT GACCACGCTG 
GAGGAGGTAC CCTCGGGTCC ACCACTTGCC GTTCGGGCTG AGCCCAAGAG CTCCACCGAG 
ATCTTCGTCA CGTGGGATGC ACCGGAACGG GATCACTGGA ACGGCATCCT GCTCGGCTAC 
TACGTGGGCT ACCAGATGTC GCTGACGCCC GAGGACAAGG AGGTGAATCC CACGCAGGGA 
TTCAGCTTCA AAACCGTCGA GGTGCGCTCT CATTTCGGTG GCGAGACGGT GCTGGCCAAT 
CTCAACAAGT TCACCCAATA CCACGTGATT GTGCAGGCCT ACACGAGCCA GGGTAGCGGA 
CCACCGAGCA AGGAGATTGC CGTGCAAACA ATGGAGGACG TTCCCTCATC GCCGCCTGAA 
TCGCCGCAGT GCGATGTCCT GGGCTCCACT TCGATCTATA TCACCTGGTC ACCGCCGGAC 
ATTGATGGCC AAAATGGAAA AATCAAGGGC TATAAAGTGT TTTACATATC GGTGGACGAG 
CTGTACGAAA CCGATCCGGA GGTTGTCAAG TCAACAAATC AATACGTCAC CATCGAGAAT 
CTGCGCAAAT ACACCAACTA CACGGTCTGG GTTTTGGCTT ACACCAAAGT AGGCGATGGC 
ATGAAAACCA AACCGTTTTA TTGCCGCACC CACGAGGATG TGCCCTCGGC CCCGCAGGCC 
ATCAAGGCGA TACCCGCGTC GAGCTCGAAA ATCATAATAT CCTGGCTGCC GCCCGACCTG 
CCCAATGGTG ACATTACCGG CTACACCTTC TACATGTCCA TGCTGGAGGG TGGACGCGAG 
GAGGGCACCC ACAAGCGCCT CCTAGGTCCC TTCGTGGAAA TGCACGAAAC GGTGCGCACC 
CAGGAGTCGG CCACGTACCA GTTCTGGCTG ACTGCCTCCA CCAAGATGGG CGAGGGCGAG 
AAGACGCAGG TGGTAACTGT GCCGCCGAAC AATAAGGTGC CCGCCCGCAT AGTGTCCTTC 
AGCCAGCGAA TAGTCACGCC CTGGAAGGAG CATCTGGAGC TGCCATGCCG GAAGGTGGGA 
GCACCTGCTC CGGTTACCAT TTGGCGCCAG GATGGTCACA ATATGGAGAC GAGTGCCCGC 
AAGACGATAG CCAAGAATGG CACGCTCTAC ATGAAGGAGT GCCAGGCGAG TGATGCGGGC 
AACTACACTT GCAGTGTGGA GAACACCTGG GGCAAGGACG AGATCGTGTA CAACATTGTG 
GTAAAGGTGC CACCAGAGGC GCCCAATCTC ACGGTGATAA ATGCCTACAC GGACAGTCTG 
CTCCTCGAAT GGATGGACAA TAGTCATGGT GGTAGTCCCA TTCTGGGTTA TGTGATCAAT 
TACAAGCGCG ACAACGGCGA TTGGGAAGAG CTGCAGGTGG ACTCGAAGAC CACGTCGCAC 
CTGCTGACCA ACCTGTGGTG TGGCACTCGC TATCAGTTGT ACATAACCGC TTACAATAAG 
ATTGGAACGG GCTTACCCTG CGACATAGTC AACTCCTATA CGAAGGGAAA TCCGCCCGTG 
CAACCGAAGC ACTCCCAGAT GATTACCAAC AACTCAACGA GCGTCACCTG CTGGCTGGAT 
TCCTGGGGAG ATGGTGGCTG TGGCATCCTG TACTTCATGA TCGAGTCCAG GGTTTATGGC 
CGATCCTCGT GGGCAGTGGT GTCCAATCAC ATTCCGCCGA CTGAGAGGAT ATACACGGTG 
AGCGATCTGG TGCCCGGCAC AAAGTACCAA CTGAAAGTCA CGGCACACAA TAATGCCGGC 
TCCACCACTG CCATCTACAA CTTTACCACA CTGTCTACAC AAGGAGTGAT TTACAACAAC 
GATCACTCCA CACCTGTATC CCACCTGAGC GACCTGCCCT TCTATGCCAA TTTCAAACTA 
CTGCTGCCCA TATGCTTTTC CCTACTGATG TTGCTGGCTT TGATTGGTGC CGCTCTATTC 
CTCAGGAAGC GAAAGCTAGC CAGCCAGGCC CGTCTGGCCA GTTCCTCGAT GTCGGAGTCG 
CCATCCCTGG CCAATCTGCA GAACAAACAG AATCGGGACC AGCAGTACCT AGCCGTGCGA 
TGCAATCCGG GCACATCTGC TCCGCGGGGA TCCAACTCCA ATGATTCCGG CAGCTTTGGC 
AAGGCGGAGG GAAACGAGTA CATCGAGGAC ATCTGCCCGT ATGCAACCTT TCAACTGAAC 
AAACAGACTT ATAGCGAGAG CTCCTACAGC GGCAATGTCT ACAGTGGGCC CTACCACTCG 
GTGCGCGGAT CCTTTGTCTA CCACGATGTC AAGCCGGAAA GCTACCACTC CAAGGAACCG 
GAGTACACCA AAGTGCGGCG AAAAGTGGGT CGCCTCAGGG ATCCTCATTC AGAGAGCCAA 
GAATCGGACA ATCCAGGTTC AACAGATTCC GAAGTTAGGA AAATCCTAAC GCTTCACATC 
CCAATTACAG AATATGACAC ACTCGGCTCC GAGTCCGACA ATGATGTGTC TGCGCGAGCT 
CTAAACTCGG CCAAGTATCG AGCACAACGT GATACTCAGG ACGAGACTTC GTCCTCCTCG 
GAAACCACAC CCACTAGCAT GACCCGGAAG TCAAAGCCGC CCTTTGCCGC CCGGAAGGGC 
GGAAAGCCCG GAACGAGCGG AAAGCGGCAC GTCCGAAGTT CCAGTGGCTA TTCGAGCCAC 
AATGAAGAAA CTACTTTTAG CATATCCAAC TATCCACCAA ACTATCAGGA CCACATAAAT 
CCACCAGCTC GTTTTTCGGA TGCCAACGAC AAGACGAAGA CCCAGACGTC GCCACGGATG 
CGTGCCAATC AGAAACTACA CCGGGAGGCT TTCCAGATAA ATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261572.1
CDS	407..6214
Translation	MDLHSLFKAILILYVIRAETSQFVNGLDLQGPIFLHEPPHRVEFSNNSGGLIECSGHGSPPPEVEWTPIPPQQDMVFQLSNGSMMFYPFTAEKYRHEVHATVYRCKLRNLVGTVLSREVHVRGVVNQKYAVQVHDEYVMTGNTAVLKCQVPSYMSEFVLVTAWVQDTGMHLYPNTDIGGKYTVLSNGELYINNAGPNDAYKSYTCRTVNRLTGEVQISTYPGRIIVTEPKGMVQPRINVEKHSMRHVVLNGQTTLPCIAQGHPVPTYRWFKEENEQLLPLQLSERITIVSAGLLKITKARLEDSGKYLCWVNNTAGEETIQVSLTVTAPLTAHLQPQVQTVDVDKDAQFQCIVSGHPVHDVNWLHDGKPILRDNRVEILTDPPRLIIKKVQKEDPGMYQCFVSNEWEQIQSTAELQLGDASPELLYWFSEQTLQPGPTVSLKCVATGNPLPQFTWSLDGFPIPDSSRFLVGQYVTIHDDVISHVNISNVKEEDGGEYTCTAQNAIGKVSHSAKVNIYGLPYIREMPKITGISGSDLIVKCPVAGYPIDKIHWERDGQTLPINRRQRAYNNGTLIIEQLQRLEDAGTYTCMAQNKQKQTSRRNVEIQVLVPPKIMPIQAMTNMLREGMRAAISCQILEGDLPVSFRWERNGKPLIGTGNEVFRRLDEYSASLVIEHISSDHSGNYTCIASNVAGTERFTVPLTVNVPPKWILEPKDSSAQAGADVLLHCQSSGYPTPTITWKKAIGPTPGEYKDFLYEPTVQLFPNGTIFFKKISKESQGHFLCEAKNNIGSGVSKVIFLKVNVPAHFQTKTKQISVAKGKQVHVQCNVQGDNPIDFKWKIQATQQYLDESLDSRYTIRDQVLDDGMVSELGISHTYRQDTGIYICQASNAFGQDEMSIQLIVQEVPEQPKNLRINSQQSRSLQLTWSQPFAGNSPIEEYHIYYKQISDIWQNAEHLTIAGAQTVINIQQLRPAKAYHIRMSAENKLGASEFSEVVQVTTLEEVPSGPPLAVRAEPKSSTEIFVTWDAPERDHWNGILLGYYVGYQMSLTPEDKEVNPTQGFSFKTVEVRSHFGGETVLANLNKFTQYHVIVQAYTSQGSGPPSKEIAVQTMEDVPSSPPESPQCDVLGSTSIYITWSPPDIDGQNGKIKGYKVFYISVDELYETDPEVVKSTNQYVTIENLRKYTNYTVWVLAYTKVGDGMKTKPFYCRTHEDVPSAPQAIKAIPASSSKIIISWLPPDLPNGDITGYTFYMSMLEGGREEGTHKRLLGPFVEMHETVRTQESATYQFWLTASTKMGEGEKTQVVTVPPNNKVPARIVSFSQRIVTPWKEHLELPCRKVGAPAPVTIWRQDGHNMETSARKTIAKNGTLYMKECQASDAGNYTCSVENTWGKDEIVYNIVVKVPPEAPNLTVINAYTDSLLLEWMDNSHGGSPILGYVINYKRDNGDWEELQVDSKTTSHLLTNLWCGTRYQLYITAYNKIGTGLPCDIVNSYTKGNPPVQPKHSQMITNNSTSVTCWLDSWGDGGCGILYFMIESRVYGRSSWAVVSNHIPPTERIYTVSDLVPGTKYQLKVTAHNNAGSTTAIYNFTTLSTQGVIYNNDHSTPVSHLSDLPFYANFKLLLPICFSLLMLLALIGAALFLRKRKLASQARLASSSMSESPSLANLQNKQNRDQQYLAVRCNPGTSAPRGSNSNDSGSFGKAEGNEYIEDICPYATFQLNKQTYSESSYSGNVYSGPYHSVRGSFVYHDVKPESYHSKEPEYTKVRRKVGRLRDPHSESQESDNPGSTDSEVRKILTLHIPITEYDTLGSESDNDVSARALNSAKYRAQRDTQDETSSSSETTPTSMTRKSKPPFAARKGGKPGTSGKRHVRSSSGYSSHNEETTFSISNYPPNYQDHINPPARFSDANDKTKTQTSPRMRANQKLHREAFQINV

Target ORF information:

RefSeq Version	NM_001274643.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster down syndrome cell adhesion molecule 4, transcript variant J (Dscam4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274643.2

ORF Insert Sequence:

ATGGATTTAC ACAGCCTTTT TAAGGCCATC CTGATCCTAT ATGTCATAAG AGCCGAGACC 
AGCCAGTTTG TAAATGGACT GGATCTACAG GGACCGATTT TTCTCCATGA GCCACCGCAT 
CGCGTCGAGT TCTCCAACAA TTCGGGCGGA CTCATAGAAT GCTCCGGGCA TGGAAGTCCC 
CCGCCGGAGG TGGAGTGGAC CCCCATCCCG CCGCAGCAGG ACATGGTGTT CCAGCTCTCC 
AACGGTAGCA TGATGTTCTA TCCCTTTACG GCCGAGAAGT ACCGCCACGA GGTGCATGCC 
ACAGTCTACA GATGCAAACT GCGCAATTTG GTGGGCACCG TGCTCAGCCG AGAGGTTCAC 
GTGCGCGGCG TCGTCAACCA GAAGTACGCG GTCCAGGTGC ACGATGAATA CGTGATGACG 
GGCAATACGG CGGTGCTGAA ATGCCAGGTT CCCAGCTACA TGTCGGAATT CGTATTGGTC 
ACCGCTTGGG TGCAGGACAC CGGCATGCAT TTGTATCCGA ACACGGACAT CGGTGGCAAG 
TACACTGTGC TATCGAACGG CGAATTGTAC ATAAATAATG CGGGACCCAA TGATGCGTAC 
AAAAGCTACA CATGCCGCAC TGTAAATAGA CTGACAGGTG AAGTACAAAT TTCCACATAT 
CCCGGCCGCA TTATTGTAAC AGAGCCCAAG GGCATGGTAC AGCCGCGCAT CAACGTGGAG 
AAGCATTCGA TGCGGCATGT TGTCCTTAAT GGCCAAACAA CGTTGCCGTG CATAGCTCAA 
GGACATCCGG TGCCAACATA TCGGTGGTTC AAGGAGGAAA ACGAGCAACT GCTGCCGCTG 
CAACTCAGCG AACGCATCAC CATCGTATCC GCAGGACTCC TCAAAATCAC CAAGGCACGT 
CTCGAGGATA GTGGAAAATA TTTGTGCTGG GTGAACAATA CGGCTGGCGA GGAGACCATC 
CAAGTGTCGC TAACGGTGAC AGCTCCTTTG ACGGCGCACC TACAGCCACA GGTGCAGACG 
GTGGATGTCG ATAAGGATGC GCAATTCCAA TGCATTGTCT CTGGCCATCC GGTCCACGAT 
GTCAACTGGC TGCACGACGG CAAACCCATA CTCAGGGACA ATCGGGTTGA GATACTCACC 
GATCCACCGC GACTGATAAT TAAAAAAGTG CAAAAGGAGG ACCCTGGCAT GTACCAGTGC 
TTCGTCTCCA ACGAATGGGA GCAGATCCAA TCCACCGCTG AACTGCAGTT GGGCGATGCC 
TCGCCGGAAC TGCTTTATTG GTTCTCGGAG CAAACGCTGC AGCCAGGACC AACGGTATCA 
TTGAAGTGCG TAGCCACCGG AAATCCACTG CCTCAATTTA CATGGTCCCT GGACGGATTT 
CCGATACCAG ACAGCTCGCG CTTTTTAGTG GGTCAATATG TTACCATCCA CGACGATGTC 
ATCAGCCACG TGAATATATC AAATGTCAAG GAGGAGGACG GCGGGGAGTA CACCTGCACG 
GCCCAGAATG CAATTGGCAA AGTCTCGCAC AGCGCCAAAG TGAACATCTA TGGATTGCCT 
TACATACGCG AAATGCCAAA AATAACAGGC ATTTCTGGCT CTGATTTGAT TGTTAAATGT 
CCCGTGGCTG GTTACCCCAT CGATAAAATT CACTGGGAGC GAGATGGCCA AACGCTGCCC 
ATAAATCGCA GGCAGCGTGC CTACAACAAT GGCACCTTAA TTATCGAGCA ACTGCAGCGC 
CTCGAGGATG CGGGCACCTA CACGTGCATG GCGCAGAACA AACAAAAGCA AACATCCCGC 
CGGAATGTGG AGATCCAAGT GCTGGTGCCG CCGAAAATTA TGCCCATCCA AGCCATGACG 
AACATGCTCC GCGAGGGAAT GCGCGCCGCC ATATCCTGCC AAATCCTCGA GGGCGACCTG 
CCCGTCAGTT TTCGATGGGA GCGCAACGGA AAGCCACTGA TCGGAACGGG CAATGAGGTT 
TTCCGCCGCC TGGATGAGTA TTCCGCCAGT TTGGTCATCG AGCACATTTC CTCCGACCAT 
TCCGGAAACT ACACCTGCAT CGCGAGCAAT GTGGCAGGTA CGGAAAGATT CACCGTACCC 
CTGACCGTGA ATGTACCGCC CAAGTGGATC CTGGAGCCAA AGGACTCGAG TGCCCAGGCG 
GGTGCAGATG TCCTTCTGCA CTGTCAATCC TCAGGATATC CCACACCGAC AATCACGTGG 
AAAAAGGCGA TTGGACCAAC GCCAGGGGAG TACAAGGATT TCCTTTACGA GCCCACAGTG 
CAGCTATTTC CCAACGGAAC CATTTTCTTC AAGAAGATCA GCAAGGAGTC GCAAGGACAC 
TTCCTGTGCG AGGCCAAAAA TAACATTGGT TCCGGTGTCA GCAAAGTCAT CTTCCTCAAA 
GTGAATGTGC CCGCCCACTT TCAGACGAAG ACCAAACAGA TTTCGGTGGC CAAAGGAAAG 
CAGGTCCATG TGCAGTGCAA CGTGCAGGGC GACAATCCCA TCGACTTTAA ATGGAAAATC 
CAGGCAACGC AACAGTATCT CGACGAGTCG CTGGATTCCC GCTACACAAT AAGAGATCAA 
GTGCTCGACG ACGGCATGGT CTCCGAATTG GGCATTTCGC ACACATATCG CCAGGATACG 
GGCATTTATA TCTGTCAGGC GAGCAATGCA TTCGGACAGG ACGAGATGTC CATTCAGCTC 
ATCGTGCAGG AAGTGCCCGA GCAGCCGAAG AACCTTCGCA TCAACTCGCA ACAGTCGCGC 
AGCCTTCAGC TCACGTGGAG CCAACCCTTT GCCGGAAATA GTCCCATCGA GGAGTACCAC 
ATCTACTACA AACAAATATC AGACATTTGG CAAAATGCGG AGCACCTCAC CATCGCCGGA 
GCCCAGACCG TGATCAACAT CCAGCAGCTG CGACCGGCAA AAGCCTATCA CATCCGCATG 
TCGGCGGAGA ACAAGCTGGG CGCCTCCGAG TTCTCGGAGG TGGTGCAAGT GACCACGCTG 
GAGGAGGTAC CCTCGGGTCC ACCACTTGCC GTTCGGGCTG AGCCCAAGAG CTCCACCGAG 
ATCTTCGTCA CGTGGGATGC ACCGGAACGG GATCACTGGA ACGGCATCCT GCTCGGCTAC 
TACGTGGGCT ACCAGATGTC GCTGACGCCC GAGGACAAGG AGGTGAATCC CACGCAGGGA 
TTCAGCTTCA AAACCGTCGA GGTGCGCTCT CATTTCGGTG GCGAGACGGT GCTGGCCAAT 
CTCAACAAGT TCACCCAATA CCACGTGATT GTGCAGGCCT ACACGAGCCA GGGTAGCGGA 
CCACCGAGCA AGGAGATTGC CGTGCAAACA ATGGAGGACG TTCCCTCATC GCCGCCTGAA 
TCGCCGCAGT GCGATGTCCT GGGCTCCACT TCGATCTATA TCACCTGGTC ACCGCCGGAC 
ATTGATGGCC AAAATGGAAA AATCAAGGGC TATAAAGTGT TTTACATATC GGTGGACGAG 
CTGTACGAAA CCGATCCGGA GGTTGTCAAG TCAACAAATC AATACGTCAC CATCGAGAAT 
CTGCGCAAAT ACACCAACTA CACGGTCTGG GTTTTGGCTT ACACCAAAGT AGGCGATGGC 
ATGAAAACCA AACCGTTTTA TTGCCGCACC CACGAGGATG TGCCCTCGGC CCCGCAGGCC 
ATCAAGGCGA TACCCGCGTC GAGCTCGAAA ATCATAATAT CCTGGCTGCC GCCCGACCTG 
CCCAATGGTG ACATTACCGG CTACACCTTC TACATGTCCA TGCTGGAGGG TGGACGCGAG 
GAGGGCACCC ACAAGCGCCT CCTAGGTCCC TTCGTGGAAA TGCACGAAAC GGTGCGCACC 
CAGGAGTCGG CCACGTACCA GTTCTGGCTG ACTGCCTCCA CCAAGATGGG CGAGGGCGAG 
AAGACGCAGG TGGTAACTGT GCCGCCGAAC AATAAGGTGC CCGCCCGCAT AGTGTCCTTC 
AGCCAGCGAA TAGTCACGCC CTGGAAGGAG CATCTGGAGC TGCCATGCCG GAAGGTGGGA 
GCACCTGCTC CGGTTACCAT TTGGCGCCAG GATGGTCACA ATATGGAGAC GAGTGCCCGC 
AAGACGATAG CCAAGAATGG CACGCTCTAC ATGAAGGAGT GCCAGGCGAG TGATGCGGGC 
AACTACACTT GCAGTGTGGA GAACACCTGG GGCAAGGACG AGATCGTGTA CAACATTGTG 
GTAAAGGTGC CACCAGAGGC GCCCAATCTC ACGGTGATAA ATGCCTACAC GGACAGTCTG 
CTCCTCGAAT GGATGGACAA TAGTCATGGT GGTAGTCCCA TTCTGGGTTA TGTGATCAAT 
TACAAGCGCG ACAACGGCGA TTGGGAAGAG CTGCAGGTGG ACTCGAAGAC CACGTCGCAC 
CTGCTGACCA ACCTGTGGTG TGGCACTCGC TATCAGTTGT ACATAACCGC TTACAATAAG 
ATTGGAACGG GCTTACCCTG CGACATAGTC AACTCCTATA CGAAGGGAAA TCCGCCCGTG 
CAACCGAAGC ACTCCCAGAT GATTACCAAC AACTCAACGA GCGTCACCTG CTGGCTGGAT 
TCCTGGGGAG ATGGTGGCTG TGGCATCCTG TACTTCATGA TCGAGTCCAG GGTTTATGGC 
CGATCCTCGT GGGCAGTGGT GTCCAATCAC ATTCCGCCGA CTGAGAGGAT ATACACGGTG 
AGCGATCTGG TGCCCGGCAC AAAGTACCAA CTGAAAGTCA CGGCACACAA TAATGCCGGC 
TCCACCACTG CCATCTACAA CTTTACCACA CTGTCTACAC AAGGAGTGAT TTACAACAAC 
GATCACTCCA CACCTGTATC CCACCTGAGC GACCTGCCCT TCTATGCCAA TTTCAAACTA 
CTGCTGCCCA TATGCTTTTC CCTACTGATG TTGCTGGCTT TGATTGGTGC CGCTCTATTC 
CTCAGGAAGC GAAAGCTAGC CAGCCAGGCC CGTCTGGCCA GTTCCTCGAT GTCGGAGTCG 
CCATCCCTGG CCAATCTGCA GAACAAACAG AATCGGGACC AGCAGTACCT AGCCGTGCGA 
TGCAATCCGG GCACATCTGC TCCGCGGGGA TCCAACTCCA ATGATTCCGG CAGCTTTGGC 
AAGGCGGAGG GAAACGAGTA CATCGAGGAC ATCTGCCCGT ATGCAACCTT TCAACTGAAC 
AAACAGACTT ATAGCGAGAG CTCCTACAGC GGCAATGTCT ACAGTGGGCC CTACCACTCG 
GTGCGCGGAT CCTTTGTCTA CCACGATGTC AAGCCGGAAA GCTACCACTC CAAGGAACCG 
GAGTACACCA AAGTGCGGCG AAAAGTGGGT CGCCTCAGGG ATCCTCATTC AGAGAGCCAA 
GAATCGGACA ATCCAGGTTC AACAGATTCC GAAGTTAGGA AAATCCTAAC GCTTCACATC 
CCAATTACAG AATATGACAC ACTCGGCTCC GAGTCCGACA ATGATGTGTC TGCGCGAGCT 
CTAAACTCGG CCAAGTATCG AGCACAACGT GATACTCAGG ACGAGACTTC GTCCTCCTCG 
GAAACCACAC CCACTAGCAT GACCCGGAAG TCAAAGCCGC CCTTTGCCGC CCGGAAGGGC 
GGAAAGCCCG GAACGAGCGG AAAGCGGCAC GTCCGAAGTT CCAGTGGCTA TTCGAGCCAC 
AATGAAGAAA CTACTTTTAG CATATCCAAC TATCCACCAA ACTATCAGGA CCACATAAAT 
CCACCAGCTC GTTTTTCGGA TGCCAACGAC AAGACGAAGA CCCAGACGTC GCCACGGATG 
CGTGCCAATC AGAAACTACA CCGGGAGGCT TTCCAGATAA ATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dscam4 ( NM_001274642.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261571.1 Drosophila melanogaster down syndrome cell adhesion molecule 4, transcript variant I (Dscam4), mRNA.

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CloneID	OFa19637
Clone ID Related Accession (Same CDS sequence)	NM_001274643.2 , NM_001274642.1
Accession Version	NM_001274642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	down syndrome cell adhesion molecule 4, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTTAC ACAGCCTTTT TAAGGCCATC CTGATCCTAT ATGTCATAAG AGCCGAGACC 
AGCCAGTTTG TAAATGGACT GGATCTACAG GGACCGATTT TTCTCCATGA GCCACCGCAT 
CGCGTCGAGT TCTCCAACAA TTCGGGCGGA CTCATAGAAT GCTCCGGGCA TGGAAGTCCC 
CCGCCGGAGG TGGAGTGGAC CCCCATCCCG CCGCAGCAGG ACATGGTGTT CCAGCTCTCC 
AACGGTAGCA TGATGTTCTA TCCCTTTACG GCCGAGAAGT ACCGCCACGA GGTGCATGCC 
ACAGTCTACA GATGCAAACT GCGCAATTTG GTGGGCACCG TGCTCAGCCG AGAGGTTCAC 
GTGCGCGGCG TCGTCAACCA GAAGTACGCG GTCCAGGTGC ACGATGAATA CGTGATGACG 
GGCAATACGG CGGTGCTGAA ATGCCAGGTT CCCAGCTACA TGTCGGAATT CGTATTGGTC 
ACCGCTTGGG TGCAGGACAC CGGCATGCAT TTGTATCCGA ACACGGACAT CGGTGGCAAG 
TACACTGTGC TATCGAACGG CGAATTGTAC ATAAATAATG CGGGACCCAA TGATGCGTAC 
AAAAGCTACA CATGCCGCAC TGTAAATAGA CTGACAGGTG AAGTACAAAT TTCCACATAT 
CCCGGCCGCA TTATTGTAAC AGAGCCCAAG GGCATGGTAC AGCCGCGCAT CAACGTGGAG 
AAGCATTCGA TGCGGCATGT TGTCCTTAAT GGCCAAACAA CGTTGCCGTG CATAGCTCAA 
GGACATCCGG TGCCAACATA TCGGTGGTTC AAGGAGGAAA ACGAGCAACT GCTGCCGCTG 
CAACTCAGCG AACGCATCAC CATCGTATCC GCAGGACTCC TCAAAATCAC CAAGGCACGT 
CTCGAGGATA GTGGAAAATA TTTGTGCTGG GTGAACAATA CGGCTGGCGA GGAGACCATC 
CAAGTGTCGC TAACGGTGAC AGCTCCTTTG ACGGCGCACC TACAGCCACA GGTGCAGACG 
GTGGATGTCG ATAAGGATGC GCAATTCCAA TGCATTGTCT CTGGCCATCC GGTCCACGAT 
GTCAACTGGC TGCACGACGG CAAACCCATA CTCAGGGACA ATCGGGTTGA GATACTCACC 
GATCCACCGC GACTGATAAT TAAAAAAGTG CAAAAGGAGG ACCCTGGCAT GTACCAGTGC 
TTCGTCTCCA ACGAATGGGA GCAGATCCAA TCCACCGCTG AACTGCAGTT GGGCGATGCC 
TCGCCGGAAC TGCTTTATTG GTTCTCGGAG CAAACGCTGC AGCCAGGACC AACGGTATCA 
TTGAAGTGCG TAGCCACCGG AAATCCACTG CCTCAATTTA CATGGTCCCT GGACGGATTT 
CCGATACCAG ACAGCTCGCG CTTTTTAGTG GGTCAATATG TTACCATCCA CGACGATGTC 
ATCAGCCACG TGAATATATC AAATGTCAAG GAGGAGGACG GCGGGGAGTA CACCTGCACG 
GCCCAGAATG CAATTGGCAA AGTCTCGCAC AGCGCCAAAG TGAACATCTA TGGATTGCCT 
TACATACGCG AAATGCCAAA AATAACAGGC ATTTCTGGCT CTGATTTGAT TGTTAAATGT 
CCCGTGGCTG GTTACCCCAT CGATAAAATT CACTGGGAGC GAGATGGCCA AACGCTGCCC 
ATAAATCGCA GGCAGCGTGC CTACAACAAT GGCACCTTAA TTATCGAGCA ACTGCAGCGC 
CTCGAGGATG CGGGCACCTA CACGTGCATG GCGCAGAACA AACAAAAGCA AACATCCCGC 
CGGAATGTGG AGATCCAAGT GCTGGTGCCG CCGAAAATTA TGCCCATCCA AGCCATGACG 
AACATGCTCC GCGAGGGAAT GCGCGCCGCC ATATCCTGCC AAATCCTCGA GGGCGACCTG 
CCCGTCAGTT TTCGATGGGA GCGCAACGGA AAGCCACTGA TCGGAACGGG CAATGAGGTT 
TTCCGCCGCC TGGATGAGTA TTCCGCCAGT TTGGTCATCG AGCACATTTC CTCCGACCAT 
TCCGGAAACT ACACCTGCAT CGCGAGCAAT GTGGCAGGTA CGGAAAGATT CACCGTACCC 
CTGACCGTGA ATGTACCGCC CAAGTGGATC CTGGAGCCAA AGGACTCGAG TGCCCAGGCG 
GGTGCAGATG TCCTTCTGCA CTGTCAATCC TCAGGATATC CCACACCGAC AATCACGTGG 
AAAAAGGCGA TTGGACCAAC GCCAGGGGAG TACAAGGATT TCCTTTACGA GCCCACAGTG 
CAGCTATTTC CCAACGGAAC CATTTTCTTC AAGAAGATCA GCAAGGAGTC GCAAGGACAC 
TTCCTGTGCG AGGCCAAAAA TAACATTGGT TCCGGTGTCA GCAAAGTCAT CTTCCTCAAA 
GTGAATGTGC CCGCCCACTT TCAGACGAAG ACCAAACAGA TTTCGGTGGC CAAAGGAAAG 
CAGGTCCATG TGCAGTGCAA CGTGCAGGGC GACAATCCCA TCGACTTTAA ATGGAAAATC 
CAGGCAACGC AACAGTATCT CGACGAGTCG CTGGATTCCC GCTACACAAT AAGAGATCAA 
GTGCTCGACG ACGGCATGGT CTCCGAATTG GGCATTTCGC ACACATATCG CCAGGATACG 
GGCATTTATA TCTGTCAGGC GAGCAATGCA TTCGGACAGG ACGAGATGTC CATTCAGCTC 
ATCGTGCAGG AAGTGCCCGA GCAGCCGAAG AACCTTCGCA TCAACTCGCA ACAGTCGCGC 
AGCCTTCAGC TCACGTGGAG CCAACCCTTT GCCGGAAATA GTCCCATCGA GGAGTACCAC 
ATCTACTACA AACAAATATC AGACATTTGG CAAAATGCGG AGCACCTCAC CATCGCCGGA 
GCCCAGACCG TGATCAACAT CCAGCAGCTG CGACCGGCAA AAGCCTATCA CATCCGCATG 
TCGGCGGAGA ACAAGCTGGG CGCCTCCGAG TTCTCGGAGG TGGTGCAAGT GACCACGCTG 
GAGGAGGTAC CCTCGGGTCC ACCACTTGCC GTTCGGGCTG AGCCCAAGAG CTCCACCGAG 
ATCTTCGTCA CGTGGGATGC ACCGGAACGG GATCACTGGA ACGGCATCCT GCTCGGCTAC 
TACGTGGGCT ACCAGATGTC GCTGACGCCC GAGGACAAGG AGGTGAATCC CACGCAGGGA 
TTCAGCTTCA AAACCGTCGA GGTGCGCTCT CATTTCGGTG GCGAGACGGT GCTGGCCAAT 
CTCAACAAGT TCACCCAATA CCACGTGATT GTGCAGGCCT ACACGAGCCA GGGTAGCGGA 
CCACCGAGCA AGGAGATTGC CGTGCAAACA ATGGAGGACG TTCCCTCATC GCCGCCTGAA 
TCGCCGCAGT GCGATGTCCT GGGCTCCACT TCGATCTATA TCACCTGGTC ACCGCCGGAC 
ATTGATGGCC AAAATGGAAA AATCAAGGGC TATAAAGTGT TTTACATATC GGTGGACGAG 
CTGTACGAAA CCGATCCGGA GGTTGTCAAG TCAACAAATC AATACGTCAC CATCGAGAAT 
CTGCGCAAAT ACACCAACTA CACGGTCTGG GTTTTGGCTT ACACCAAAGT AGGCGATGGC 
ATGAAAACCA AACCGTTTTA TTGCCGCACC CACGAGGATG TGCCCTCGGC CCCGCAGGCC 
ATCAAGGCGA TACCCGCGTC GAGCTCGAAA ATCATAATAT CCTGGCTGCC GCCCGACCTG 
CCCAATGGTG ACATTACCGG CTACACCTTC TACATGTCCA TGCTGGAGGG TGGACGCGAG 
GAGGGCACCC ACAAGCGCCT CCTAGGTCCC TTCGTGGAAA TGCACGAAAC GGTGCGCACC 
CAGGAGTCGG CCACGTACCA GTTCTGGCTG ACTGCCTCCA CCAAGATGGG CGAGGGCGAG 
AAGACGCAGG TGGTAACTGT GCCGCCGAAC AATAAGGTGC CCGCCCGCAT AGTGTCCTTC 
AGCCAGCGAA TAGTCACGCC CTGGAAGGAG CATCTGGAGC TGCCATGCCG GAAGGTGGGA 
GCACCTGCTC CGGTTACCAT TTGGCGCCAG GATGGTCACA ATATGGAGAC GAGTGCCCGC 
AAGACGATAG CCAAGAATGG CACGCTCTAC ATGAAGGAGT GCCAGGCGAG TGATGCGGGC 
AACTACACTT GCAGTGTGGA GAACACCTGG GGCAAGGACG AGATCGTGTA CAACATTGTG 
GTAAAGGTGC CACCAGAGGC GCCCAATCTC ACGGTGATAA ATGCCTACAC GGACAGTCTG 
CTCCTCGAAT GGATGGACAA TAGTCATGGT GGTAGTCCCA TTCTGGGTTA TGTGATCAAT 
TACAAGCGCG ACAACGGCGA TTGGGAAGAG CTGCAGGTGG ACTCGAAGAC CACGTCGCAC 
CTGCTGACCA ACCTGTGGTG TGGCACTCGC TATCAGTTGT ACATAACCGC TTACAATAAG 
ATTGGAACGG GCTTACCCTG CGACATAGTC AACTCCTATA CGAAGGGAAA TCCGCCCGTG 
CAACCGAAGC ACTCCCAGAT GATTACCAAC AACTCAACGA GCGTCACCTG CTGGCTGGAT 
TCCTGGGGAG ATGGTGGCTG TGGCATCCTG TACTTCATGA TCGAGTCCAG GGTTTATGGC 
CGATCCTCGT GGGCAGTGGT GTCCAATCAC ATTCCGCCGA CTGAGAGGAT ATACACGGTG 
AGCGATCTGG TGCCCGGCAC AAAGTACCAA CTGAAAGTCA CGGCACACAA TAATGCCGGC 
TCCACCACTG CCATCTACAA CTTTACCACA CTGTCTACAC AAGGAGTGAT TTACAACAAC 
GATCACTCCA CACCTGTATC CCACCTGAGC GACCTGCCCT TCTATGCCAA TTTCAAACTA 
CTGCTGCCCA TATGCTTTTC CCTACTGATG TTGCTGGCTT TGATTGGTGC CGCTCTATTC 
CTCAGGAAGC GAAAGCTAGC CAGCCAGGCC CGTCTGGCCA GTTCCTCGAT GTCGGAGTCG 
CCATCCCTGG CCAATCTGCA GAACAAACAG AATCGGGACC AGCAGTACCT AGCCGTGCGA 
TGCAATCCGG GCACATCTGC TCCGCGGGGA TCCAACTCCA ATGATTCCGG CAGCTTTGGC 
AAGGCGGAGG GAAACGAGTA CATCGAGGAC ATCTGCCCGT ATGCAACCTT TCAACTGAAC 
AAACAGACTT ATAGCGAGAG CTCCTACAGC GGCAATGTCT ACAGTGGGCC CTACCACTCG 
GTGCGCGGAT CCTTTGTCTA CCACGATGTC AAGCCGGAAA GCTACCACTC CAAGGAACCG 
GAGTACACCA AAGTGCGGCG AAAAGTGGGT CGCCTCAGGG ATCCTCATTC AGAGAGCCAA 
GAATCGGACA ATCCAGGTTC AACAGATTCC GAAGTTAGGA AAATCCTAAC GCTTCACATC 
CCAATTACAG AATATGACAC ACTCGGCTCC GAGTCCGACA ATGATGTGTC TGCGCGAGCT 
CTAAACTCGG CCAAGTATCG AGCACAACGT GATACTCAGG ACGAGACTTC GTCCTCCTCG 
GAAACCACAC CCACTAGCAT GACCCGGAAG TCAAAGCCGC CCTTTGCCGC CCGGAAGGGC 
GGAAAGCCCG GAACGAGCGG AAAGCGGCAC GTCCGAAGTT CCAGTGGCTA TTCGAGCCAC 
AATGAAGAAA CTACTTTTAG CATATCCAAC TATCCACCAA ACTATCAGGA CCACATAAAT 
CCACCAGCTC GTTTTTCGGA TGCCAACGAC AAGACGAAGA CCCAGACGTC GCCACGGATG 
CGTGCCAATC AGAAACTACA CCGGGAGGCT TTCCAGATAA ATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261571.1
CDS	517..6324
Translation	MDLHSLFKAILILYVIRAETSQFVNGLDLQGPIFLHEPPHRVEFSNNSGGLIECSGHGSPPPEVEWTPIPPQQDMVFQLSNGSMMFYPFTAEKYRHEVHATVYRCKLRNLVGTVLSREVHVRGVVNQKYAVQVHDEYVMTGNTAVLKCQVPSYMSEFVLVTAWVQDTGMHLYPNTDIGGKYTVLSNGELYINNAGPNDAYKSYTCRTVNRLTGEVQISTYPGRIIVTEPKGMVQPRINVEKHSMRHVVLNGQTTLPCIAQGHPVPTYRWFKEENEQLLPLQLSERITIVSAGLLKITKARLEDSGKYLCWVNNTAGEETIQVSLTVTAPLTAHLQPQVQTVDVDKDAQFQCIVSGHPVHDVNWLHDGKPILRDNRVEILTDPPRLIIKKVQKEDPGMYQCFVSNEWEQIQSTAELQLGDASPELLYWFSEQTLQPGPTVSLKCVATGNPLPQFTWSLDGFPIPDSSRFLVGQYVTIHDDVISHVNISNVKEEDGGEYTCTAQNAIGKVSHSAKVNIYGLPYIREMPKITGISGSDLIVKCPVAGYPIDKIHWERDGQTLPINRRQRAYNNGTLIIEQLQRLEDAGTYTCMAQNKQKQTSRRNVEIQVLVPPKIMPIQAMTNMLREGMRAAISCQILEGDLPVSFRWERNGKPLIGTGNEVFRRLDEYSASLVIEHISSDHSGNYTCIASNVAGTERFTVPLTVNVPPKWILEPKDSSAQAGADVLLHCQSSGYPTPTITWKKAIGPTPGEYKDFLYEPTVQLFPNGTIFFKKISKESQGHFLCEAKNNIGSGVSKVIFLKVNVPAHFQTKTKQISVAKGKQVHVQCNVQGDNPIDFKWKIQATQQYLDESLDSRYTIRDQVLDDGMVSELGISHTYRQDTGIYICQASNAFGQDEMSIQLIVQEVPEQPKNLRINSQQSRSLQLTWSQPFAGNSPIEEYHIYYKQISDIWQNAEHLTIAGAQTVINIQQLRPAKAYHIRMSAENKLGASEFSEVVQVTTLEEVPSGPPLAVRAEPKSSTEIFVTWDAPERDHWNGILLGYYVGYQMSLTPEDKEVNPTQGFSFKTVEVRSHFGGETVLANLNKFTQYHVIVQAYTSQGSGPPSKEIAVQTMEDVPSSPPESPQCDVLGSTSIYITWSPPDIDGQNGKIKGYKVFYISVDELYETDPEVVKSTNQYVTIENLRKYTNYTVWVLAYTKVGDGMKTKPFYCRTHEDVPSAPQAIKAIPASSSKIIISWLPPDLPNGDITGYTFYMSMLEGGREEGTHKRLLGPFVEMHETVRTQESATYQFWLTASTKMGEGEKTQVVTVPPNNKVPARIVSFSQRIVTPWKEHLELPCRKVGAPAPVTIWRQDGHNMETSARKTIAKNGTLYMKECQASDAGNYTCSVENTWGKDEIVYNIVVKVPPEAPNLTVINAYTDSLLLEWMDNSHGGSPILGYVINYKRDNGDWEELQVDSKTTSHLLTNLWCGTRYQLYITAYNKIGTGLPCDIVNSYTKGNPPVQPKHSQMITNNSTSVTCWLDSWGDGGCGILYFMIESRVYGRSSWAVVSNHIPPTERIYTVSDLVPGTKYQLKVTAHNNAGSTTAIYNFTTLSTQGVIYNNDHSTPVSHLSDLPFYANFKLLLPICFSLLMLLALIGAALFLRKRKLASQARLASSSMSESPSLANLQNKQNRDQQYLAVRCNPGTSAPRGSNSNDSGSFGKAEGNEYIEDICPYATFQLNKQTYSESSYSGNVYSGPYHSVRGSFVYHDVKPESYHSKEPEYTKVRRKVGRLRDPHSESQESDNPGSTDSEVRKILTLHIPITEYDTLGSESDNDVSARALNSAKYRAQRDTQDETSSSSETTPTSMTRKSKPPFAARKGGKPGTSGKRHVRSSSGYSSHNEETTFSISNYPPNYQDHINPPARFSDANDKTKTQTSPRMRANQKLHREAFQINV

Target ORF information:

RefSeq Version	NM_001274642.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster down syndrome cell adhesion molecule 4, transcript variant I (Dscam4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274642.1

ORF Insert Sequence:

ATGGATTTAC ACAGCCTTTT TAAGGCCATC CTGATCCTAT ATGTCATAAG AGCCGAGACC 
AGCCAGTTTG TAAATGGACT GGATCTACAG GGACCGATTT TTCTCCATGA GCCACCGCAT 
CGCGTCGAGT TCTCCAACAA TTCGGGCGGA CTCATAGAAT GCTCCGGGCA TGGAAGTCCC 
CCGCCGGAGG TGGAGTGGAC CCCCATCCCG CCGCAGCAGG ACATGGTGTT CCAGCTCTCC 
AACGGTAGCA TGATGTTCTA TCCCTTTACG GCCGAGAAGT ACCGCCACGA GGTGCATGCC 
ACAGTCTACA GATGCAAACT GCGCAATTTG GTGGGCACCG TGCTCAGCCG AGAGGTTCAC 
GTGCGCGGCG TCGTCAACCA GAAGTACGCG GTCCAGGTGC ACGATGAATA CGTGATGACG 
GGCAATACGG CGGTGCTGAA ATGCCAGGTT CCCAGCTACA TGTCGGAATT CGTATTGGTC 
ACCGCTTGGG TGCAGGACAC CGGCATGCAT TTGTATCCGA ACACGGACAT CGGTGGCAAG 
TACACTGTGC TATCGAACGG CGAATTGTAC ATAAATAATG CGGGACCCAA TGATGCGTAC 
AAAAGCTACA CATGCCGCAC TGTAAATAGA CTGACAGGTG AAGTACAAAT TTCCACATAT 
CCCGGCCGCA TTATTGTAAC AGAGCCCAAG GGCATGGTAC AGCCGCGCAT CAACGTGGAG 
AAGCATTCGA TGCGGCATGT TGTCCTTAAT GGCCAAACAA CGTTGCCGTG CATAGCTCAA 
GGACATCCGG TGCCAACATA TCGGTGGTTC AAGGAGGAAA ACGAGCAACT GCTGCCGCTG 
CAACTCAGCG AACGCATCAC CATCGTATCC GCAGGACTCC TCAAAATCAC CAAGGCACGT 
CTCGAGGATA GTGGAAAATA TTTGTGCTGG GTGAACAATA CGGCTGGCGA GGAGACCATC 
CAAGTGTCGC TAACGGTGAC AGCTCCTTTG ACGGCGCACC TACAGCCACA GGTGCAGACG 
GTGGATGTCG ATAAGGATGC GCAATTCCAA TGCATTGTCT CTGGCCATCC GGTCCACGAT 
GTCAACTGGC TGCACGACGG CAAACCCATA CTCAGGGACA ATCGGGTTGA GATACTCACC 
GATCCACCGC GACTGATAAT TAAAAAAGTG CAAAAGGAGG ACCCTGGCAT GTACCAGTGC 
TTCGTCTCCA ACGAATGGGA GCAGATCCAA TCCACCGCTG AACTGCAGTT GGGCGATGCC 
TCGCCGGAAC TGCTTTATTG GTTCTCGGAG CAAACGCTGC AGCCAGGACC AACGGTATCA 
TTGAAGTGCG TAGCCACCGG AAATCCACTG CCTCAATTTA CATGGTCCCT GGACGGATTT 
CCGATACCAG ACAGCTCGCG CTTTTTAGTG GGTCAATATG TTACCATCCA CGACGATGTC 
ATCAGCCACG TGAATATATC AAATGTCAAG GAGGAGGACG GCGGGGAGTA CACCTGCACG 
GCCCAGAATG CAATTGGCAA AGTCTCGCAC AGCGCCAAAG TGAACATCTA TGGATTGCCT 
TACATACGCG AAATGCCAAA AATAACAGGC ATTTCTGGCT CTGATTTGAT TGTTAAATGT 
CCCGTGGCTG GTTACCCCAT CGATAAAATT CACTGGGAGC GAGATGGCCA AACGCTGCCC 
ATAAATCGCA GGCAGCGTGC CTACAACAAT GGCACCTTAA TTATCGAGCA ACTGCAGCGC 
CTCGAGGATG CGGGCACCTA CACGTGCATG GCGCAGAACA AACAAAAGCA AACATCCCGC 
CGGAATGTGG AGATCCAAGT GCTGGTGCCG CCGAAAATTA TGCCCATCCA AGCCATGACG 
AACATGCTCC GCGAGGGAAT GCGCGCCGCC ATATCCTGCC AAATCCTCGA GGGCGACCTG 
CCCGTCAGTT TTCGATGGGA GCGCAACGGA AAGCCACTGA TCGGAACGGG CAATGAGGTT 
TTCCGCCGCC TGGATGAGTA TTCCGCCAGT TTGGTCATCG AGCACATTTC CTCCGACCAT 
TCCGGAAACT ACACCTGCAT CGCGAGCAAT GTGGCAGGTA CGGAAAGATT CACCGTACCC 
CTGACCGTGA ATGTACCGCC CAAGTGGATC CTGGAGCCAA AGGACTCGAG TGCCCAGGCG 
GGTGCAGATG TCCTTCTGCA CTGTCAATCC TCAGGATATC CCACACCGAC AATCACGTGG 
AAAAAGGCGA TTGGACCAAC GCCAGGGGAG TACAAGGATT TCCTTTACGA GCCCACAGTG 
CAGCTATTTC CCAACGGAAC CATTTTCTTC AAGAAGATCA GCAAGGAGTC GCAAGGACAC 
TTCCTGTGCG AGGCCAAAAA TAACATTGGT TCCGGTGTCA GCAAAGTCAT CTTCCTCAAA 
GTGAATGTGC CCGCCCACTT TCAGACGAAG ACCAAACAGA TTTCGGTGGC CAAAGGAAAG 
CAGGTCCATG TGCAGTGCAA CGTGCAGGGC GACAATCCCA TCGACTTTAA ATGGAAAATC 
CAGGCAACGC AACAGTATCT CGACGAGTCG CTGGATTCCC GCTACACAAT AAGAGATCAA 
GTGCTCGACG ACGGCATGGT CTCCGAATTG GGCATTTCGC ACACATATCG CCAGGATACG 
GGCATTTATA TCTGTCAGGC GAGCAATGCA TTCGGACAGG ACGAGATGTC CATTCAGCTC 
ATCGTGCAGG AAGTGCCCGA GCAGCCGAAG AACCTTCGCA TCAACTCGCA ACAGTCGCGC 
AGCCTTCAGC TCACGTGGAG CCAACCCTTT GCCGGAAATA GTCCCATCGA GGAGTACCAC 
ATCTACTACA AACAAATATC AGACATTTGG CAAAATGCGG AGCACCTCAC CATCGCCGGA 
GCCCAGACCG TGATCAACAT CCAGCAGCTG CGACCGGCAA AAGCCTATCA CATCCGCATG 
TCGGCGGAGA ACAAGCTGGG CGCCTCCGAG TTCTCGGAGG TGGTGCAAGT GACCACGCTG 
GAGGAGGTAC CCTCGGGTCC ACCACTTGCC GTTCGGGCTG AGCCCAAGAG CTCCACCGAG 
ATCTTCGTCA CGTGGGATGC ACCGGAACGG GATCACTGGA ACGGCATCCT GCTCGGCTAC 
TACGTGGGCT ACCAGATGTC GCTGACGCCC GAGGACAAGG AGGTGAATCC CACGCAGGGA 
TTCAGCTTCA AAACCGTCGA GGTGCGCTCT CATTTCGGTG GCGAGACGGT GCTGGCCAAT 
CTCAACAAGT TCACCCAATA CCACGTGATT GTGCAGGCCT ACACGAGCCA GGGTAGCGGA 
CCACCGAGCA AGGAGATTGC CGTGCAAACA ATGGAGGACG TTCCCTCATC GCCGCCTGAA 
TCGCCGCAGT GCGATGTCCT GGGCTCCACT TCGATCTATA TCACCTGGTC ACCGCCGGAC 
ATTGATGGCC AAAATGGAAA AATCAAGGGC TATAAAGTGT TTTACATATC GGTGGACGAG 
CTGTACGAAA CCGATCCGGA GGTTGTCAAG TCAACAAATC AATACGTCAC CATCGAGAAT 
CTGCGCAAAT ACACCAACTA CACGGTCTGG GTTTTGGCTT ACACCAAAGT AGGCGATGGC 
ATGAAAACCA AACCGTTTTA TTGCCGCACC CACGAGGATG TGCCCTCGGC CCCGCAGGCC 
ATCAAGGCGA TACCCGCGTC GAGCTCGAAA ATCATAATAT CCTGGCTGCC GCCCGACCTG 
CCCAATGGTG ACATTACCGG CTACACCTTC TACATGTCCA TGCTGGAGGG TGGACGCGAG 
GAGGGCACCC ACAAGCGCCT CCTAGGTCCC TTCGTGGAAA TGCACGAAAC GGTGCGCACC 
CAGGAGTCGG CCACGTACCA GTTCTGGCTG ACTGCCTCCA CCAAGATGGG CGAGGGCGAG 
AAGACGCAGG TGGTAACTGT GCCGCCGAAC AATAAGGTGC CCGCCCGCAT AGTGTCCTTC 
AGCCAGCGAA TAGTCACGCC CTGGAAGGAG CATCTGGAGC TGCCATGCCG GAAGGTGGGA 
GCACCTGCTC CGGTTACCAT TTGGCGCCAG GATGGTCACA ATATGGAGAC GAGTGCCCGC 
AAGACGATAG CCAAGAATGG CACGCTCTAC ATGAAGGAGT GCCAGGCGAG TGATGCGGGC 
AACTACACTT GCAGTGTGGA GAACACCTGG GGCAAGGACG AGATCGTGTA CAACATTGTG 
GTAAAGGTGC CACCAGAGGC GCCCAATCTC ACGGTGATAA ATGCCTACAC GGACAGTCTG 
CTCCTCGAAT GGATGGACAA TAGTCATGGT GGTAGTCCCA TTCTGGGTTA TGTGATCAAT 
TACAAGCGCG ACAACGGCGA TTGGGAAGAG CTGCAGGTGG ACTCGAAGAC CACGTCGCAC 
CTGCTGACCA ACCTGTGGTG TGGCACTCGC TATCAGTTGT ACATAACCGC TTACAATAAG 
ATTGGAACGG GCTTACCCTG CGACATAGTC AACTCCTATA CGAAGGGAAA TCCGCCCGTG 
CAACCGAAGC ACTCCCAGAT GATTACCAAC AACTCAACGA GCGTCACCTG CTGGCTGGAT 
TCCTGGGGAG ATGGTGGCTG TGGCATCCTG TACTTCATGA TCGAGTCCAG GGTTTATGGC 
CGATCCTCGT GGGCAGTGGT GTCCAATCAC ATTCCGCCGA CTGAGAGGAT ATACACGGTG 
AGCGATCTGG TGCCCGGCAC AAAGTACCAA CTGAAAGTCA CGGCACACAA TAATGCCGGC 
TCCACCACTG CCATCTACAA CTTTACCACA CTGTCTACAC AAGGAGTGAT TTACAACAAC 
GATCACTCCA CACCTGTATC CCACCTGAGC GACCTGCCCT TCTATGCCAA TTTCAAACTA 
CTGCTGCCCA TATGCTTTTC CCTACTGATG TTGCTGGCTT TGATTGGTGC CGCTCTATTC 
CTCAGGAAGC GAAAGCTAGC CAGCCAGGCC CGTCTGGCCA GTTCCTCGAT GTCGGAGTCG 
CCATCCCTGG CCAATCTGCA GAACAAACAG AATCGGGACC AGCAGTACCT AGCCGTGCGA 
TGCAATCCGG GCACATCTGC TCCGCGGGGA TCCAACTCCA ATGATTCCGG CAGCTTTGGC 
AAGGCGGAGG GAAACGAGTA CATCGAGGAC ATCTGCCCGT ATGCAACCTT TCAACTGAAC 
AAACAGACTT ATAGCGAGAG CTCCTACAGC GGCAATGTCT ACAGTGGGCC CTACCACTCG 
GTGCGCGGAT CCTTTGTCTA CCACGATGTC AAGCCGGAAA GCTACCACTC CAAGGAACCG 
GAGTACACCA AAGTGCGGCG AAAAGTGGGT CGCCTCAGGG ATCCTCATTC AGAGAGCCAA 
GAATCGGACA ATCCAGGTTC AACAGATTCC GAAGTTAGGA AAATCCTAAC GCTTCACATC 
CCAATTACAG AATATGACAC ACTCGGCTCC GAGTCCGACA ATGATGTGTC TGCGCGAGCT 
CTAAACTCGG CCAAGTATCG AGCACAACGT GATACTCAGG ACGAGACTTC GTCCTCCTCG 
GAAACCACAC CCACTAGCAT GACCCGGAAG TCAAAGCCGC CCTTTGCCGC CCGGAAGGGC 
GGAAAGCCCG GAACGAGCGG AAAGCGGCAC GTCCGAAGTT CCAGTGGCTA TTCGAGCCAC 
AATGAAGAAA CTACTTTTAG CATATCCAAC TATCCACCAA ACTATCAGGA CCACATAAAT 
CCACCAGCTC GTTTTTCGGA TGCCAACGAC AAGACGAAGA CCCAGACGTC GCCACGGATG 
CGTGCCAATC AGAAACTACA CCGGGAGGCT TTCCAGATAA ATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dscam4 ( NM_001043131.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036596.2 Drosophila melanogaster down syndrome cell adhesion molecule 4, transcript variant E (Dscam4), mRNA.

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CloneID	OFa20093
Clone ID Related Accession (Same CDS sequence)	NM_001043131.3 , NM_001144436.2
Accession Version	NM_001043131.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	down syndrome cell adhesion molecule 4, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001043131.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTTAC ACAGCCTTTT TAAGGCCATC CTGATCCTAT ATGTCATAAG AGCCGAGACC 
AGCCAGTTTG TAAATGGACT GGATCTACAG GGACCGATTT TTCTCCATGA GCCACCGCAT 
CGCGTCGAGT TCTCCAACAA TTCGGGCGGA CTCATAGAAT GCTCCGGGCA TGGAAGTCCC 
CCGCCGGAGG TGGAGTGGAC CCCCATCCCG CCGCAGCAGG ACATGGTGTT CCAGCTCTCC 
AACGGTAGCA TGATGTTCTA TCCCTTTACG GCCGAGAAGT ACCGCCACGA GGTGCATGCC 
ACAGTCTACA GATGCAAACT GCGCAATTTG GTGGGCACCG TGCTCAGCCG AGAGGTTCAC 
GTGCGCGGCG TCGTCAACCA GAAGTACGCG GTCCAGGTGC ACGATGAATA CGTGATGACG 
GGCAATACGG CGGTGCTGAA ATGCCAGGTT CCCAGCTACA TGTCGGAATT CGTATTGGTC 
ACCGCTTGGG TGCAGGACAC CGGCATGCAT TTGTATCCGA ACACGGACAT CGGTGGCAAG 
TACACTGTGC TATCGAACGG CGAATTGTAC ATAAATAATG CGGGACCCAA TGATGCGTAC 
AAAAGCTACA CATGCCGCAC TGTAAATAGA CTGACAGGTG AAGTACAAAT TTCCACATAT 
CCCGGCCGCA TTATTGTAAC AGAGCCCAAG GGCATGGTAC AGCCGCGCAT CAACGTGGAG 
AAGCATTCGA TGCGGCATGT TGTCCTTAAT GGCCAAACAA CGTTGCCGTG CATAGCTCAA 
GGACATCCGG TGCCAACATA TCGGTGGTTC AAGGAGGAAA ACGAGCAACT GCTGCCGCTG 
CAACTCAGCG AACGCATCAC CATCGTATCC GCAGGACTCC TCAAAATCAC CAAGGCACGT 
CTCGAGGATA GTGGAAAATA TTTGTGCTGG GTGAACAATA CGGCTGGCGA GGAGACCATC 
CAAGTGTCGC TAACGGTGAC AGCTCCTTTG ACGGCGCACC TACAGCCACA GGTGCAGACG 
GTGGATGTCG ATAAGGATGC GCAATTCCAA TGCATTGTCT CTGGCCATCC GGTCCACGAT 
GTCAACTGGC TGCACGACGG CAAACCCATA CTCAGGGACA ATCGGGTTGA GATACTCACC 
GATCCACCGC GACTGATAAT TAAAAAAGTG CAAAAGGAGG ACCCTGGCAT GTACCAGTGC 
TTCGTCTCCA ACGAATGGGA GCAGATCCAA TCCACCGCTG AACTGCAGTT GGGCGATGCC 
TCGCCGGAAC TGCTTTATTG GTTCTCGGAG CAAACGCTGC AGCCAGGACC AACGGTATCA 
TTGAAGTGCG TAGCCACCGG AAATCCACTG CCTCAATTTA CATGGTCCCT GGACGGATTT 
CCGATACCAG ACAGCTCGCG CTTTTTAGTG GGTCAATATG TTACCATCCA CGACGATGTC 
ATCAGCCACG TGAATATATC AAATGTCAAG GAGGAGGACG GCGGGGAGTA CACCTGCACG 
GCCCAGAATG CAATTGGCAA AGTCTCGCAC AGCGCCAAAG TGAACATCTA TGGATTGCCT 
TACATACGCG AAATGCCAAA AATAACAGGC ATTTCTGGCT CTGATTTGAT TGTTAAATGT 
CCCGTGGCTG GTTACCCCAT CGATAAAATT CACTGGGAGC GAGATGGCCA AACGCTGCCC 
ATAAATCGCA GGCAGCGTGC CTACAACAAT GGCACCTTAA TTATCGAGCA ACTGCAGCGC 
CTCGAGGATG CGGGCACCTA CACGTGCATG GCGCAGAACA AACAAAAGCA AACATCCCGC 
CGGAATGTGG AGATCCAAGT GCTGGTGCCG CCGAAAATTA TGCCCATCCA AGCCATGACG 
AACATGCTCC GCGAGGGAAT GCGCGCCGCC ATATCCTGCC AAATCCTCGA GGGCGACCTG 
CCCGTCAGTT TTCGATGGGA GCGCAACGGA AAGCCACTGA TCGGAACGGG CAATGAGGTT 
TTCCGCCGCC TGGATGAGTA TTCCGCCAGT TTGGTCATCG AGCACATTTC CTCCGACCAT 
TCCGGAAACT ACACCTGCAT CGCGAGCAAT GTGGCAGGTA CGGAAAGATT CACCGTACCC 
CTGACCGTGA ATGTACCGCC CAAGTGGATC CTGGAGCCAA AGGACTCGAG TGCCCAGGCG 
GGTGCAGATG TCCTTCTGCA CTGTCAATCC TCAGGATATC CCACACCGAC AATCACGTGG 
AAAAAGGCGA TTGGACCAAC GCCAGGGGAG TACAAGGATT TCCTTTACGA GCCCACAGTG 
CAGCTATTTC CCAACGGAAC CATTTTCTTC AAGAAGATCA GCAAGGAGTC GCAAGGACAC 
TTCCTGTGCG AGGCCAAAAA TAACATTGGT TCCGGTGTCA GCAAAGTCAT CTTCCTCAAA 
GTGAATGTGC CCGCCCACTT TCAGACGAAG ACCAAACAGA TTTCGGTGGC CAAAGGAAAG 
CAGGTCCATG TGCAGTGCAA CGTGCAGGGC GACAATCCCA TCGACTTTAA ATGGAAAATC 
CAGGCAACGC AACAGTATCT CGACGAGTCG CTGGATTCCC GCTACACAAT AAGAGATCAA 
GTGCTCGACG ACGGCATGGT CTCCGAATTG GGCATTTCGC ACACATATCG CCAGGATACG 
GGCATTTATA TCTGTCAGGC GAGCAATGCA TTCGGACAGG ACGAGATGTC CATTCAGCTC 
ATCGTGCAGG AAGTGCCCGA GCAGCCGAAG AACCTTCGCA TCAACTCGCA ACAGTCGCGC 
AGCCTTCAGC TCACGTGGAG CCAACCCTTT GCCGGAAATA GTCCCATCGA GGAGTACCAC 
ATCTACTACA AACAAATATC AGATTTCTTT TCACCCTCAG ACATTTGGCA AAATGCGGAG 
CACCTCACCA TCGCCGGAGC CCAGACCGTG ATCAACATCC AGCAGCTGCG ACCGGCAAAA 
GCCTATCACA TCCGCATGTC GGCGGAGAAC AAGCTGGGCG CCTCCGAGTT CTCGGAGGTG 
GTGCAAGTGA CCACGCTGGA GGAGGTACCC TCGGGTCCAC CACTTGCCGT TCGGGCTGAG 
CCCAAGAGCT CCACCGAGAT CTTCGTCACG TGGGATGCAC CGGAACGGGA TCACTGGAAC 
GGCATCCTGC TCGGCTACTA CGTGGGCTAC CAGATGTCGC TGACGCCCGA GGACAAGGAG 
GTGAATCCCA CGCAGGGATT CAGCTTCAAA ACCGTCGAGG TGCGCTCTCA TTTCGGTGGC 
GAGACGGTGC TGGCCAATCT CAACAAGTTC ACCCAATACC ACGTGATTGT GCAGGCCTAC 
ACGAGCCAGG GTAGCGGACC ACCGAGCAAG GAGATTGCCG TGCAAACAAT GGAGGACGTT 
CCCTCATCGC CGCCTGAATC GCCGCAGTGC GATGTCCTGG GCTCCACTTC GATCTATATC 
ACCTGGTCAC CGCCGGACAT TGATGGCCAA AATGGAAAAA TCAAGGGCTA TAAAGTGTTT 
TACATATCGG TGGACGAGCT GTACGAAACC GATCCGGAGG TTGTCAAGTC AACAAATCAA 
TACGTCACCA TCGAGAATCT GCGCAAATAC ACCAACTACA CGGTCTGGGT TTTGGCTTAC 
ACCAAAGTAG GCGATGGCAT GAAAACCAAA CCGTTTTATT GCCGCACCCA CGAGGATGTG 
CCCTCGGCCC CGCAGGCCAT CAAGGCGATA CCCGCGTCGA GCTCGAAAAT CATAATATCC 
TGGCTGCCGC CCGACCTGCC CAATGGTGAC ATTACCGGCT ACACCTTCTA CATGTCCATG 
CTGGAGGGTG GACGCGAGGA GGGCACCCAC AAGCGCCTCC TAGGTCCCTT CGTGGAAATG 
CACGAAACGG TGCGCACCCA GGAGTCGGCC ACGTACCAGT TCTGGCTGAC TGCCTCCACC 
AAGATGGGCG AGGGCGAGAA GACGCAGGTG GTAACTGTGC CGCCGAACAA TAAGGTGCCC 
GCCCGCATAG TGTCCTTCAG CCAGCGAATA GTCACGCCCT GGAAGGAGCA TCTGGAGCTG 
CCATGCCGGA AGGTGGGAGC ACCTGCTCCG GTTACCATTT GGCGCCAGGA TGGTCACAAT 
ATGGAGACGA GTGCCCGCAA GACGATAGCC AAGAATGGCA CGCTCTACAT GAAGGAGTGC 
CAGGCGAGTG ATGCGGGCAA CTACACTTGC AGTGTGGAGA ACACCTGGGG CAAGGACGAG 
ATCGTGTACA ACATTGTGGT AAAGGTGCCA CCAGAGGCGC CCAATCTCAC GGTGATAAAT 
GCCTACACGG ACAGTCTGCT CCTCGAATGG ATGGACAATA GTCATGGTGG TAGTCCCATT 
CTGGGTTATG TGATCAATTA CAAGCGCGAC AACGGCGATT GGGAAGAGCT GCAGGTGGAC 
TCGAAGACCA CGTCGCACCT GCTGACCAAC CTGTGGTGTG GCACTCGCTA TCAGTTGTAC 
ATAACCGCTT ACAATAAGAT TGGAACGGGC TTACCCTGCG ACATAGTCAA CTCCTATACG 
AAGGGAAATC CGCCCGTGCA ACCGAAGCAC TCCCAGATGA TTACCAACAA CTCAACGAGC 
GTCACCTGCT GGCTGGATTC CTGGGGAGAT GGTGGCTGTG GCATCCTGTA CTTCATGATC 
GAGTCCAGGG TTTATGGCCG ATCCTCGTGG GCAGTGGTGT CCAATCACAT TCCGCCGACT 
GAGAGGATAT ACACGGTGAG CGATCTGGTG CCCGGCACAA AGTACCAACT GAAAGTCACG 
GCACACAATA ATGCCGGCTC CACCACTGCC ATCTACAACT TTACCACACT GTCTACACAA 
GGAGTGATTT ACAACAACGA TCACTCCACA CCTGTATCCC ACCTGAGCGA CCTGCCCTTC 
TATGCCAATT TCAAACTACT GCTGCCCATA TGCTTTTCCC TACTGATGTT GCTGGCTTTG 
ATTGGTGCCG CTCTATTCCT CAGGAAGCGA AAGCTAGCCA GCCAGGCCCG TCTGGCCAGT 
TCCTCGATGT CGGAGTCGCC ATCCCTGGCC AATCTGCAGA ACAAACAGAA TCGGGACCAG 
CAGTACCTAG CCGTGCGATG CAATCCGGGC ACATCTGCTC CGCGGGGATC CAACTCCAAT 
GATTCCGGCA GCTTTGGCAA GGCGGAGGGA AACGAGTACA TCGAGGACAT CTGCCCGTAT 
GCAACCTTTC AACTGAACAA ACAGACTTAT AGCGAGAGCT CCTACAGCGG CAATGTCTAC 
AGTGGGCCCT ACCACTCGGT GCGCGGATCC TTTGTCTACC ACGATGTCAA GCCGGAAAGC 
TACCACTCCA AGGAACCGGA GTACACCAAA GTGCGGCGAA AAGTGGGTCG CCTCAGGGAT 
CCTCATTCAG AGAGCCAAGA ATATGACACA CTCGGCTCCG AGTCCGACAA TGATGTGTCT 
GCGCGAGCTC TAAACTCGGC CAAGTATCGA GCACAACGTG ATACTCAGGA CGAGACTTCG 
TCCTCCTCGG AAACCACACC CACTAGCATG ACCCGGAAGT CAAAGCCGCC CTTTGCCGCC 
CGGAAGGGCG GAAAGCCCGG AACGAGCGGA AAGCGGCACG TCCGAAGTTC CAGTGGCTAT 
TCGAGCCACA ATGAAGAAAC TACTTTTAGC ATATCCAACT ATCCACCAAA CTATCAGGAC 
CACATAAATC CACCAGCTCG TTTTTCGGAT GCCAACGACA AGACGAAGAC CCAGACGTCG 
CCACGGATGC GTGCCAATCA GAAACTACAC CGGGAGGCTT TCCAGATAAA TGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036596.2
CDS	233..5989
Translation	MDLHSLFKAILILYVIRAETSQFVNGLDLQGPIFLHEPPHRVEFSNNSGGLIECSGHGSPPPEVEWTPIPPQQDMVFQLSNGSMMFYPFTAEKYRHEVHATVYRCKLRNLVGTVLSREVHVRGVVNQKYAVQVHDEYVMTGNTAVLKCQVPSYMSEFVLVTAWVQDTGMHLYPNTDIGGKYTVLSNGELYINNAGPNDAYKSYTCRTVNRLTGEVQISTYPGRIIVTEPKGMVQPRINVEKHSMRHVVLNGQTTLPCIAQGHPVPTYRWFKEENEQLLPLQLSERITIVSAGLLKITKARLEDSGKYLCWVNNTAGEETIQVSLTVTAPLTAHLQPQVQTVDVDKDAQFQCIVSGHPVHDVNWLHDGKPILRDNRVEILTDPPRLIIKKVQKEDPGMYQCFVSNEWEQIQSTAELQLGDASPELLYWFSEQTLQPGPTVSLKCVATGNPLPQFTWSLDGFPIPDSSRFLVGQYVTIHDDVISHVNISNVKEEDGGEYTCTAQNAIGKVSHSAKVNIYGLPYIREMPKITGISGSDLIVKCPVAGYPIDKIHWERDGQTLPINRRQRAYNNGTLIIEQLQRLEDAGTYTCMAQNKQKQTSRRNVEIQVLVPPKIMPIQAMTNMLREGMRAAISCQILEGDLPVSFRWERNGKPLIGTGNEVFRRLDEYSASLVIEHISSDHSGNYTCIASNVAGTERFTVPLTVNVPPKWILEPKDSSAQAGADVLLHCQSSGYPTPTITWKKAIGPTPGEYKDFLYEPTVQLFPNGTIFFKKISKESQGHFLCEAKNNIGSGVSKVIFLKVNVPAHFQTKTKQISVAKGKQVHVQCNVQGDNPIDFKWKIQATQQYLDESLDSRYTIRDQVLDDGMVSELGISHTYRQDTGIYICQASNAFGQDEMSIQLIVQEVPEQPKNLRINSQQSRSLQLTWSQPFAGNSPIEEYHIYYKQISDFFSPSDIWQNAEHLTIAGAQTVINIQQLRPAKAYHIRMSAENKLGASEFSEVVQVTTLEEVPSGPPLAVRAEPKSSTEIFVTWDAPERDHWNGILLGYYVGYQMSLTPEDKEVNPTQGFSFKTVEVRSHFGGETVLANLNKFTQYHVIVQAYTSQGSGPPSKEIAVQTMEDVPSSPPESPQCDVLGSTSIYITWSPPDIDGQNGKIKGYKVFYISVDELYETDPEVVKSTNQYVTIENLRKYTNYTVWVLAYTKVGDGMKTKPFYCRTHEDVPSAPQAIKAIPASSSKIIISWLPPDLPNGDITGYTFYMSMLEGGREEGTHKRLLGPFVEMHETVRTQESATYQFWLTASTKMGEGEKTQVVTVPPNNKVPARIVSFSQRIVTPWKEHLELPCRKVGAPAPVTIWRQDGHNMETSARKTIAKNGTLYMKECQASDAGNYTCSVENTWGKDEIVYNIVVKVPPEAPNLTVINAYTDSLLLEWMDNSHGGSPILGYVINYKRDNGDWEELQVDSKTTSHLLTNLWCGTRYQLYITAYNKIGTGLPCDIVNSYTKGNPPVQPKHSQMITNNSTSVTCWLDSWGDGGCGILYFMIESRVYGRSSWAVVSNHIPPTERIYTVSDLVPGTKYQLKVTAHNNAGSTTAIYNFTTLSTQGVIYNNDHSTPVSHLSDLPFYANFKLLLPICFSLLMLLALIGAALFLRKRKLASQARLASSSMSESPSLANLQNKQNRDQQYLAVRCNPGTSAPRGSNSNDSGSFGKAEGNEYIEDICPYATFQLNKQTYSESSYSGNVYSGPYHSVRGSFVYHDVKPESYHSKEPEYTKVRRKVGRLRDPHSESQEYDTLGSESDNDVSARALNSAKYRAQRDTQDETSSSSETTPTSMTRKSKPPFAARKGGKPGTSGKRHVRSSSGYSSHNEETTFSISNYPPNYQDHINPPARFSDANDKTKTQTSPRMRANQKLHREAFQINV

Target ORF information:

RefSeq Version	NM_001043131.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster down syndrome cell adhesion molecule 4, transcript variant E (Dscam4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043131.3

ORF Insert Sequence:

ATGGATTTAC ACAGCCTTTT TAAGGCCATC CTGATCCTAT ATGTCATAAG AGCCGAGACC 
AGCCAGTTTG TAAATGGACT GGATCTACAG GGACCGATTT TTCTCCATGA GCCACCGCAT 
CGCGTCGAGT TCTCCAACAA TTCGGGCGGA CTCATAGAAT GCTCCGGGCA TGGAAGTCCC 
CCGCCGGAGG TGGAGTGGAC CCCCATCCCG CCGCAGCAGG ACATGGTGTT CCAGCTCTCC 
AACGGTAGCA TGATGTTCTA TCCCTTTACG GCCGAGAAGT ACCGCCACGA GGTGCATGCC 
ACAGTCTACA GATGCAAACT GCGCAATTTG GTGGGCACCG TGCTCAGCCG AGAGGTTCAC 
GTGCGCGGCG TCGTCAACCA GAAGTACGCG GTCCAGGTGC ACGATGAATA CGTGATGACG 
GGCAATACGG CGGTGCTGAA ATGCCAGGTT CCCAGCTACA TGTCGGAATT CGTATTGGTC 
ACCGCTTGGG TGCAGGACAC CGGCATGCAT TTGTATCCGA ACACGGACAT CGGTGGCAAG 
TACACTGTGC TATCGAACGG CGAATTGTAC ATAAATAATG CGGGACCCAA TGATGCGTAC 
AAAAGCTACA CATGCCGCAC TGTAAATAGA CTGACAGGTG AAGTACAAAT TTCCACATAT 
CCCGGCCGCA TTATTGTAAC AGAGCCCAAG GGCATGGTAC AGCCGCGCAT CAACGTGGAG 
AAGCATTCGA TGCGGCATGT TGTCCTTAAT GGCCAAACAA CGTTGCCGTG CATAGCTCAA 
GGACATCCGG TGCCAACATA TCGGTGGTTC AAGGAGGAAA ACGAGCAACT GCTGCCGCTG 
CAACTCAGCG AACGCATCAC CATCGTATCC GCAGGACTCC TCAAAATCAC CAAGGCACGT 
CTCGAGGATA GTGGAAAATA TTTGTGCTGG GTGAACAATA CGGCTGGCGA GGAGACCATC 
CAAGTGTCGC TAACGGTGAC AGCTCCTTTG ACGGCGCACC TACAGCCACA GGTGCAGACG 
GTGGATGTCG ATAAGGATGC GCAATTCCAA TGCATTGTCT CTGGCCATCC GGTCCACGAT 
GTCAACTGGC TGCACGACGG CAAACCCATA CTCAGGGACA ATCGGGTTGA GATACTCACC 
GATCCACCGC GACTGATAAT TAAAAAAGTG CAAAAGGAGG ACCCTGGCAT GTACCAGTGC 
TTCGTCTCCA ACGAATGGGA GCAGATCCAA TCCACCGCTG AACTGCAGTT GGGCGATGCC 
TCGCCGGAAC TGCTTTATTG GTTCTCGGAG CAAACGCTGC AGCCAGGACC AACGGTATCA 
TTGAAGTGCG TAGCCACCGG AAATCCACTG CCTCAATTTA CATGGTCCCT GGACGGATTT 
CCGATACCAG ACAGCTCGCG CTTTTTAGTG GGTCAATATG TTACCATCCA CGACGATGTC 
ATCAGCCACG TGAATATATC AAATGTCAAG GAGGAGGACG GCGGGGAGTA CACCTGCACG 
GCCCAGAATG CAATTGGCAA AGTCTCGCAC AGCGCCAAAG TGAACATCTA TGGATTGCCT 
TACATACGCG AAATGCCAAA AATAACAGGC ATTTCTGGCT CTGATTTGAT TGTTAAATGT 
CCCGTGGCTG GTTACCCCAT CGATAAAATT CACTGGGAGC GAGATGGCCA AACGCTGCCC 
ATAAATCGCA GGCAGCGTGC CTACAACAAT GGCACCTTAA TTATCGAGCA ACTGCAGCGC 
CTCGAGGATG CGGGCACCTA CACGTGCATG GCGCAGAACA AACAAAAGCA AACATCCCGC 
CGGAATGTGG AGATCCAAGT GCTGGTGCCG CCGAAAATTA TGCCCATCCA AGCCATGACG 
AACATGCTCC GCGAGGGAAT GCGCGCCGCC ATATCCTGCC AAATCCTCGA GGGCGACCTG 
CCCGTCAGTT TTCGATGGGA GCGCAACGGA AAGCCACTGA TCGGAACGGG CAATGAGGTT 
TTCCGCCGCC TGGATGAGTA TTCCGCCAGT TTGGTCATCG AGCACATTTC CTCCGACCAT 
TCCGGAAACT ACACCTGCAT CGCGAGCAAT GTGGCAGGTA CGGAAAGATT CACCGTACCC 
CTGACCGTGA ATGTACCGCC CAAGTGGATC CTGGAGCCAA AGGACTCGAG TGCCCAGGCG 
GGTGCAGATG TCCTTCTGCA CTGTCAATCC TCAGGATATC CCACACCGAC AATCACGTGG 
AAAAAGGCGA TTGGACCAAC GCCAGGGGAG TACAAGGATT TCCTTTACGA GCCCACAGTG 
CAGCTATTTC CCAACGGAAC CATTTTCTTC AAGAAGATCA GCAAGGAGTC GCAAGGACAC 
TTCCTGTGCG AGGCCAAAAA TAACATTGGT TCCGGTGTCA GCAAAGTCAT CTTCCTCAAA 
GTGAATGTGC CCGCCCACTT TCAGACGAAG ACCAAACAGA TTTCGGTGGC CAAAGGAAAG 
CAGGTCCATG TGCAGTGCAA CGTGCAGGGC GACAATCCCA TCGACTTTAA ATGGAAAATC 
CAGGCAACGC AACAGTATCT CGACGAGTCG CTGGATTCCC GCTACACAAT AAGAGATCAA 
GTGCTCGACG ACGGCATGGT CTCCGAATTG GGCATTTCGC ACACATATCG CCAGGATACG 
GGCATTTATA TCTGTCAGGC GAGCAATGCA TTCGGACAGG ACGAGATGTC CATTCAGCTC 
ATCGTGCAGG AAGTGCCCGA GCAGCCGAAG AACCTTCGCA TCAACTCGCA ACAGTCGCGC 
AGCCTTCAGC TCACGTGGAG CCAACCCTTT GCCGGAAATA GTCCCATCGA GGAGTACCAC 
ATCTACTACA AACAAATATC AGATTTCTTT TCACCCTCAG ACATTTGGCA AAATGCGGAG 
CACCTCACCA TCGCCGGAGC CCAGACCGTG ATCAACATCC AGCAGCTGCG ACCGGCAAAA 
GCCTATCACA TCCGCATGTC GGCGGAGAAC AAGCTGGGCG CCTCCGAGTT CTCGGAGGTG 
GTGCAAGTGA CCACGCTGGA GGAGGTACCC TCGGGTCCAC CACTTGCCGT TCGGGCTGAG 
CCCAAGAGCT CCACCGAGAT CTTCGTCACG TGGGATGCAC CGGAACGGGA TCACTGGAAC 
GGCATCCTGC TCGGCTACTA CGTGGGCTAC CAGATGTCGC TGACGCCCGA GGACAAGGAG 
GTGAATCCCA CGCAGGGATT CAGCTTCAAA ACCGTCGAGG TGCGCTCTCA TTTCGGTGGC 
GAGACGGTGC TGGCCAATCT CAACAAGTTC ACCCAATACC ACGTGATTGT GCAGGCCTAC 
ACGAGCCAGG GTAGCGGACC ACCGAGCAAG GAGATTGCCG TGCAAACAAT GGAGGACGTT 
CCCTCATCGC CGCCTGAATC GCCGCAGTGC GATGTCCTGG GCTCCACTTC GATCTATATC 
ACCTGGTCAC CGCCGGACAT TGATGGCCAA AATGGAAAAA TCAAGGGCTA TAAAGTGTTT 
TACATATCGG TGGACGAGCT GTACGAAACC GATCCGGAGG TTGTCAAGTC AACAAATCAA 
TACGTCACCA TCGAGAATCT GCGCAAATAC ACCAACTACA CGGTCTGGGT TTTGGCTTAC 
ACCAAAGTAG GCGATGGCAT GAAAACCAAA CCGTTTTATT GCCGCACCCA CGAGGATGTG 
CCCTCGGCCC CGCAGGCCAT CAAGGCGATA CCCGCGTCGA GCTCGAAAAT CATAATATCC 
TGGCTGCCGC CCGACCTGCC CAATGGTGAC ATTACCGGCT ACACCTTCTA CATGTCCATG 
CTGGAGGGTG GACGCGAGGA GGGCACCCAC AAGCGCCTCC TAGGTCCCTT CGTGGAAATG 
CACGAAACGG TGCGCACCCA GGAGTCGGCC ACGTACCAGT TCTGGCTGAC TGCCTCCACC 
AAGATGGGCG AGGGCGAGAA GACGCAGGTG GTAACTGTGC CGCCGAACAA TAAGGTGCCC 
GCCCGCATAG TGTCCTTCAG CCAGCGAATA GTCACGCCCT GGAAGGAGCA TCTGGAGCTG 
CCATGCCGGA AGGTGGGAGC ACCTGCTCCG GTTACCATTT GGCGCCAGGA TGGTCACAAT 
ATGGAGACGA GTGCCCGCAA GACGATAGCC AAGAATGGCA CGCTCTACAT GAAGGAGTGC 
CAGGCGAGTG ATGCGGGCAA CTACACTTGC AGTGTGGAGA ACACCTGGGG CAAGGACGAG 
ATCGTGTACA ACATTGTGGT AAAGGTGCCA CCAGAGGCGC CCAATCTCAC GGTGATAAAT 
GCCTACACGG ACAGTCTGCT CCTCGAATGG ATGGACAATA GTCATGGTGG TAGTCCCATT 
CTGGGTTATG TGATCAATTA CAAGCGCGAC AACGGCGATT GGGAAGAGCT GCAGGTGGAC 
TCGAAGACCA CGTCGCACCT GCTGACCAAC CTGTGGTGTG GCACTCGCTA TCAGTTGTAC 
ATAACCGCTT ACAATAAGAT TGGAACGGGC TTACCCTGCG ACATAGTCAA CTCCTATACG 
AAGGGAAATC CGCCCGTGCA ACCGAAGCAC TCCCAGATGA TTACCAACAA CTCAACGAGC 
GTCACCTGCT GGCTGGATTC CTGGGGAGAT GGTGGCTGTG GCATCCTGTA CTTCATGATC 
GAGTCCAGGG TTTATGGCCG ATCCTCGTGG GCAGTGGTGT CCAATCACAT TCCGCCGACT 
GAGAGGATAT ACACGGTGAG CGATCTGGTG CCCGGCACAA AGTACCAACT GAAAGTCACG 
GCACACAATA ATGCCGGCTC CACCACTGCC ATCTACAACT TTACCACACT GTCTACACAA 
GGAGTGATTT ACAACAACGA TCACTCCACA CCTGTATCCC ACCTGAGCGA CCTGCCCTTC 
TATGCCAATT TCAAACTACT GCTGCCCATA TGCTTTTCCC TACTGATGTT GCTGGCTTTG 
ATTGGTGCCG CTCTATTCCT CAGGAAGCGA AAGCTAGCCA GCCAGGCCCG TCTGGCCAGT 
TCCTCGATGT CGGAGTCGCC ATCCCTGGCC AATCTGCAGA ACAAACAGAA TCGGGACCAG 
CAGTACCTAG CCGTGCGATG CAATCCGGGC ACATCTGCTC CGCGGGGATC CAACTCCAAT 
GATTCCGGCA GCTTTGGCAA GGCGGAGGGA AACGAGTACA TCGAGGACAT CTGCCCGTAT 
GCAACCTTTC AACTGAACAA ACAGACTTAT AGCGAGAGCT CCTACAGCGG CAATGTCTAC 
AGTGGGCCCT ACCACTCGGT GCGCGGATCC TTTGTCTACC ACGATGTCAA GCCGGAAAGC 
TACCACTCCA AGGAACCGGA GTACACCAAA GTGCGGCGAA AAGTGGGTCG CCTCAGGGAT 
CCTCATTCAG AGAGCCAAGA ATATGACACA CTCGGCTCCG AGTCCGACAA TGATGTGTCT 
GCGCGAGCTC TAAACTCGGC CAAGTATCGA GCACAACGTG ATACTCAGGA CGAGACTTCG 
TCCTCCTCGG AAACCACACC CACTAGCATG ACCCGGAAGT CAAAGCCGCC CTTTGCCGCC 
CGGAAGGGCG GAAAGCCCGG AACGAGCGGA AAGCGGCACG TCCGAAGTTC CAGTGGCTAT 
TCGAGCCACA ATGAAGAAAC TACTTTTAGC ATATCCAACT ATCCACCAAA CTATCAGGAC 
CACATAAATC CACCAGCTCG TTTTTCGGAT GCCAACGACA AGACGAAGAC CCAGACGTCG 
CCACGGATGC GTGCCAATCA GAAACTACAC CGGGAGGCTT TCCAGATAAA TGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dscam4 ( NM_001144436.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137908.1 Drosophila melanogaster down syndrome cell adhesion molecule 4, transcript variant D (Dscam4), mRNA.

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CloneID	OFa20093
Clone ID Related Accession (Same CDS sequence)	NM_001043131.3 , NM_001144436.2
Accession Version	NM_001144436.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	down syndrome cell adhesion molecule 4, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001144436.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTTAC ACAGCCTTTT TAAGGCCATC CTGATCCTAT ATGTCATAAG AGCCGAGACC 
AGCCAGTTTG TAAATGGACT GGATCTACAG GGACCGATTT TTCTCCATGA GCCACCGCAT 
CGCGTCGAGT TCTCCAACAA TTCGGGCGGA CTCATAGAAT GCTCCGGGCA TGGAAGTCCC 
CCGCCGGAGG TGGAGTGGAC CCCCATCCCG CCGCAGCAGG ACATGGTGTT CCAGCTCTCC 
AACGGTAGCA TGATGTTCTA TCCCTTTACG GCCGAGAAGT ACCGCCACGA GGTGCATGCC 
ACAGTCTACA GATGCAAACT GCGCAATTTG GTGGGCACCG TGCTCAGCCG AGAGGTTCAC 
GTGCGCGGCG TCGTCAACCA GAAGTACGCG GTCCAGGTGC ACGATGAATA CGTGATGACG 
GGCAATACGG CGGTGCTGAA ATGCCAGGTT CCCAGCTACA TGTCGGAATT CGTATTGGTC 
ACCGCTTGGG TGCAGGACAC CGGCATGCAT TTGTATCCGA ACACGGACAT CGGTGGCAAG 
TACACTGTGC TATCGAACGG CGAATTGTAC ATAAATAATG CGGGACCCAA TGATGCGTAC 
AAAAGCTACA CATGCCGCAC TGTAAATAGA CTGACAGGTG AAGTACAAAT TTCCACATAT 
CCCGGCCGCA TTATTGTAAC AGAGCCCAAG GGCATGGTAC AGCCGCGCAT CAACGTGGAG 
AAGCATTCGA TGCGGCATGT TGTCCTTAAT GGCCAAACAA CGTTGCCGTG CATAGCTCAA 
GGACATCCGG TGCCAACATA TCGGTGGTTC AAGGAGGAAA ACGAGCAACT GCTGCCGCTG 
CAACTCAGCG AACGCATCAC CATCGTATCC GCAGGACTCC TCAAAATCAC CAAGGCACGT 
CTCGAGGATA GTGGAAAATA TTTGTGCTGG GTGAACAATA CGGCTGGCGA GGAGACCATC 
CAAGTGTCGC TAACGGTGAC AGCTCCTTTG ACGGCGCACC TACAGCCACA GGTGCAGACG 
GTGGATGTCG ATAAGGATGC GCAATTCCAA TGCATTGTCT CTGGCCATCC GGTCCACGAT 
GTCAACTGGC TGCACGACGG CAAACCCATA CTCAGGGACA ATCGGGTTGA GATACTCACC 
GATCCACCGC GACTGATAAT TAAAAAAGTG CAAAAGGAGG ACCCTGGCAT GTACCAGTGC 
TTCGTCTCCA ACGAATGGGA GCAGATCCAA TCCACCGCTG AACTGCAGTT GGGCGATGCC 
TCGCCGGAAC TGCTTTATTG GTTCTCGGAG CAAACGCTGC AGCCAGGACC AACGGTATCA 
TTGAAGTGCG TAGCCACCGG AAATCCACTG CCTCAATTTA CATGGTCCCT GGACGGATTT 
CCGATACCAG ACAGCTCGCG CTTTTTAGTG GGTCAATATG TTACCATCCA CGACGATGTC 
ATCAGCCACG TGAATATATC AAATGTCAAG GAGGAGGACG GCGGGGAGTA CACCTGCACG 
GCCCAGAATG CAATTGGCAA AGTCTCGCAC AGCGCCAAAG TGAACATCTA TGGATTGCCT 
TACATACGCG AAATGCCAAA AATAACAGGC ATTTCTGGCT CTGATTTGAT TGTTAAATGT 
CCCGTGGCTG GTTACCCCAT CGATAAAATT CACTGGGAGC GAGATGGCCA AACGCTGCCC 
ATAAATCGCA GGCAGCGTGC CTACAACAAT GGCACCTTAA TTATCGAGCA ACTGCAGCGC 
CTCGAGGATG CGGGCACCTA CACGTGCATG GCGCAGAACA AACAAAAGCA AACATCCCGC 
CGGAATGTGG AGATCCAAGT GCTGGTGCCG CCGAAAATTA TGCCCATCCA AGCCATGACG 
AACATGCTCC GCGAGGGAAT GCGCGCCGCC ATATCCTGCC AAATCCTCGA GGGCGACCTG 
CCCGTCAGTT TTCGATGGGA GCGCAACGGA AAGCCACTGA TCGGAACGGG CAATGAGGTT 
TTCCGCCGCC TGGATGAGTA TTCCGCCAGT TTGGTCATCG AGCACATTTC CTCCGACCAT 
TCCGGAAACT ACACCTGCAT CGCGAGCAAT GTGGCAGGTA CGGAAAGATT CACCGTACCC 
CTGACCGTGA ATGTACCGCC CAAGTGGATC CTGGAGCCAA AGGACTCGAG TGCCCAGGCG 
GGTGCAGATG TCCTTCTGCA CTGTCAATCC TCAGGATATC CCACACCGAC AATCACGTGG 
AAAAAGGCGA TTGGACCAAC GCCAGGGGAG TACAAGGATT TCCTTTACGA GCCCACAGTG 
CAGCTATTTC CCAACGGAAC CATTTTCTTC AAGAAGATCA GCAAGGAGTC GCAAGGACAC 
TTCCTGTGCG AGGCCAAAAA TAACATTGGT TCCGGTGTCA GCAAAGTCAT CTTCCTCAAA 
GTGAATGTGC CCGCCCACTT TCAGACGAAG ACCAAACAGA TTTCGGTGGC CAAAGGAAAG 
CAGGTCCATG TGCAGTGCAA CGTGCAGGGC GACAATCCCA TCGACTTTAA ATGGAAAATC 
CAGGCAACGC AACAGTATCT CGACGAGTCG CTGGATTCCC GCTACACAAT AAGAGATCAA 
GTGCTCGACG ACGGCATGGT CTCCGAATTG GGCATTTCGC ACACATATCG CCAGGATACG 
GGCATTTATA TCTGTCAGGC GAGCAATGCA TTCGGACAGG ACGAGATGTC CATTCAGCTC 
ATCGTGCAGG AAGTGCCCGA GCAGCCGAAG AACCTTCGCA TCAACTCGCA ACAGTCGCGC 
AGCCTTCAGC TCACGTGGAG CCAACCCTTT GCCGGAAATA GTCCCATCGA GGAGTACCAC 
ATCTACTACA AACAAATATC AGATTTCTTT TCACCCTCAG ACATTTGGCA AAATGCGGAG 
CACCTCACCA TCGCCGGAGC CCAGACCGTG ATCAACATCC AGCAGCTGCG ACCGGCAAAA 
GCCTATCACA TCCGCATGTC GGCGGAGAAC AAGCTGGGCG CCTCCGAGTT CTCGGAGGTG 
GTGCAAGTGA CCACGCTGGA GGAGGTACCC TCGGGTCCAC CACTTGCCGT TCGGGCTGAG 
CCCAAGAGCT CCACCGAGAT CTTCGTCACG TGGGATGCAC CGGAACGGGA TCACTGGAAC 
GGCATCCTGC TCGGCTACTA CGTGGGCTAC CAGATGTCGC TGACGCCCGA GGACAAGGAG 
GTGAATCCCA CGCAGGGATT CAGCTTCAAA ACCGTCGAGG TGCGCTCTCA TTTCGGTGGC 
GAGACGGTGC TGGCCAATCT CAACAAGTTC ACCCAATACC ACGTGATTGT GCAGGCCTAC 
ACGAGCCAGG GTAGCGGACC ACCGAGCAAG GAGATTGCCG TGCAAACAAT GGAGGACGTT 
CCCTCATCGC CGCCTGAATC GCCGCAGTGC GATGTCCTGG GCTCCACTTC GATCTATATC 
ACCTGGTCAC CGCCGGACAT TGATGGCCAA AATGGAAAAA TCAAGGGCTA TAAAGTGTTT 
TACATATCGG TGGACGAGCT GTACGAAACC GATCCGGAGG TTGTCAAGTC AACAAATCAA 
TACGTCACCA TCGAGAATCT GCGCAAATAC ACCAACTACA CGGTCTGGGT TTTGGCTTAC 
ACCAAAGTAG GCGATGGCAT GAAAACCAAA CCGTTTTATT GCCGCACCCA CGAGGATGTG 
CCCTCGGCCC CGCAGGCCAT CAAGGCGATA CCCGCGTCGA GCTCGAAAAT CATAATATCC 
TGGCTGCCGC CCGACCTGCC CAATGGTGAC ATTACCGGCT ACACCTTCTA CATGTCCATG 
CTGGAGGGTG GACGCGAGGA GGGCACCCAC AAGCGCCTCC TAGGTCCCTT CGTGGAAATG 
CACGAAACGG TGCGCACCCA GGAGTCGGCC ACGTACCAGT TCTGGCTGAC TGCCTCCACC 
AAGATGGGCG AGGGCGAGAA GACGCAGGTG GTAACTGTGC CGCCGAACAA TAAGGTGCCC 
GCCCGCATAG TGTCCTTCAG CCAGCGAATA GTCACGCCCT GGAAGGAGCA TCTGGAGCTG 
CCATGCCGGA AGGTGGGAGC ACCTGCTCCG GTTACCATTT GGCGCCAGGA TGGTCACAAT 
ATGGAGACGA GTGCCCGCAA GACGATAGCC AAGAATGGCA CGCTCTACAT GAAGGAGTGC 
CAGGCGAGTG ATGCGGGCAA CTACACTTGC AGTGTGGAGA ACACCTGGGG CAAGGACGAG 
ATCGTGTACA ACATTGTGGT AAAGGTGCCA CCAGAGGCGC CCAATCTCAC GGTGATAAAT 
GCCTACACGG ACAGTCTGCT CCTCGAATGG ATGGACAATA GTCATGGTGG TAGTCCCATT 
CTGGGTTATG TGATCAATTA CAAGCGCGAC AACGGCGATT GGGAAGAGCT GCAGGTGGAC 
TCGAAGACCA CGTCGCACCT GCTGACCAAC CTGTGGTGTG GCACTCGCTA TCAGTTGTAC 
ATAACCGCTT ACAATAAGAT TGGAACGGGC TTACCCTGCG ACATAGTCAA CTCCTATACG 
AAGGGAAATC CGCCCGTGCA ACCGAAGCAC TCCCAGATGA TTACCAACAA CTCAACGAGC 
GTCACCTGCT GGCTGGATTC CTGGGGAGAT GGTGGCTGTG GCATCCTGTA CTTCATGATC 
GAGTCCAGGG TTTATGGCCG ATCCTCGTGG GCAGTGGTGT CCAATCACAT TCCGCCGACT 
GAGAGGATAT ACACGGTGAG CGATCTGGTG CCCGGCACAA AGTACCAACT GAAAGTCACG 
GCACACAATA ATGCCGGCTC CACCACTGCC ATCTACAACT TTACCACACT GTCTACACAA 
GGAGTGATTT ACAACAACGA TCACTCCACA CCTGTATCCC ACCTGAGCGA CCTGCCCTTC 
TATGCCAATT TCAAACTACT GCTGCCCATA TGCTTTTCCC TACTGATGTT GCTGGCTTTG 
ATTGGTGCCG CTCTATTCCT CAGGAAGCGA AAGCTAGCCA GCCAGGCCCG TCTGGCCAGT 
TCCTCGATGT CGGAGTCGCC ATCCCTGGCC AATCTGCAGA ACAAACAGAA TCGGGACCAG 
CAGTACCTAG CCGTGCGATG CAATCCGGGC ACATCTGCTC CGCGGGGATC CAACTCCAAT 
GATTCCGGCA GCTTTGGCAA GGCGGAGGGA AACGAGTACA TCGAGGACAT CTGCCCGTAT 
GCAACCTTTC AACTGAACAA ACAGACTTAT AGCGAGAGCT CCTACAGCGG CAATGTCTAC 
AGTGGGCCCT ACCACTCGGT GCGCGGATCC TTTGTCTACC ACGATGTCAA GCCGGAAAGC 
TACCACTCCA AGGAACCGGA GTACACCAAA GTGCGGCGAA AAGTGGGTCG CCTCAGGGAT 
CCTCATTCAG AGAGCCAAGA ATATGACACA CTCGGCTCCG AGTCCGACAA TGATGTGTCT 
GCGCGAGCTC TAAACTCGGC CAAGTATCGA GCACAACGTG ATACTCAGGA CGAGACTTCG 
TCCTCCTCGG AAACCACACC CACTAGCATG ACCCGGAAGT CAAAGCCGCC CTTTGCCGCC 
CGGAAGGGCG GAAAGCCCGG AACGAGCGGA AAGCGGCACG TCCGAAGTTC CAGTGGCTAT 
TCGAGCCACA ATGAAGAAAC TACTTTTAGC ATATCCAACT ATCCACCAAA CTATCAGGAC 
CACATAAATC CACCAGCTCG TTTTTCGGAT GCCAACGACA AGACGAAGAC CCAGACGTCG 
CCACGGATGC GTGCCAATCA GAAACTACAC CGGGAGGCTT TCCAGATAAA TGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137908.1
CDS	517..6273
Translation	MDLHSLFKAILILYVIRAETSQFVNGLDLQGPIFLHEPPHRVEFSNNSGGLIECSGHGSPPPEVEWTPIPPQQDMVFQLSNGSMMFYPFTAEKYRHEVHATVYRCKLRNLVGTVLSREVHVRGVVNQKYAVQVHDEYVMTGNTAVLKCQVPSYMSEFVLVTAWVQDTGMHLYPNTDIGGKYTVLSNGELYINNAGPNDAYKSYTCRTVNRLTGEVQISTYPGRIIVTEPKGMVQPRINVEKHSMRHVVLNGQTTLPCIAQGHPVPTYRWFKEENEQLLPLQLSERITIVSAGLLKITKARLEDSGKYLCWVNNTAGEETIQVSLTVTAPLTAHLQPQVQTVDVDKDAQFQCIVSGHPVHDVNWLHDGKPILRDNRVEILTDPPRLIIKKVQKEDPGMYQCFVSNEWEQIQSTAELQLGDASPELLYWFSEQTLQPGPTVSLKCVATGNPLPQFTWSLDGFPIPDSSRFLVGQYVTIHDDVISHVNISNVKEEDGGEYTCTAQNAIGKVSHSAKVNIYGLPYIREMPKITGISGSDLIVKCPVAGYPIDKIHWERDGQTLPINRRQRAYNNGTLIIEQLQRLEDAGTYTCMAQNKQKQTSRRNVEIQVLVPPKIMPIQAMTNMLREGMRAAISCQILEGDLPVSFRWERNGKPLIGTGNEVFRRLDEYSASLVIEHISSDHSGNYTCIASNVAGTERFTVPLTVNVPPKWILEPKDSSAQAGADVLLHCQSSGYPTPTITWKKAIGPTPGEYKDFLYEPTVQLFPNGTIFFKKISKESQGHFLCEAKNNIGSGVSKVIFLKVNVPAHFQTKTKQISVAKGKQVHVQCNVQGDNPIDFKWKIQATQQYLDESLDSRYTIRDQVLDDGMVSELGISHTYRQDTGIYICQASNAFGQDEMSIQLIVQEVPEQPKNLRINSQQSRSLQLTWSQPFAGNSPIEEYHIYYKQISDFFSPSDIWQNAEHLTIAGAQTVINIQQLRPAKAYHIRMSAENKLGASEFSEVVQVTTLEEVPSGPPLAVRAEPKSSTEIFVTWDAPERDHWNGILLGYYVGYQMSLTPEDKEVNPTQGFSFKTVEVRSHFGGETVLANLNKFTQYHVIVQAYTSQGSGPPSKEIAVQTMEDVPSSPPESPQCDVLGSTSIYITWSPPDIDGQNGKIKGYKVFYISVDELYETDPEVVKSTNQYVTIENLRKYTNYTVWVLAYTKVGDGMKTKPFYCRTHEDVPSAPQAIKAIPASSSKIIISWLPPDLPNGDITGYTFYMSMLEGGREEGTHKRLLGPFVEMHETVRTQESATYQFWLTASTKMGEGEKTQVVTVPPNNKVPARIVSFSQRIVTPWKEHLELPCRKVGAPAPVTIWRQDGHNMETSARKTIAKNGTLYMKECQASDAGNYTCSVENTWGKDEIVYNIVVKVPPEAPNLTVINAYTDSLLLEWMDNSHGGSPILGYVINYKRDNGDWEELQVDSKTTSHLLTNLWCGTRYQLYITAYNKIGTGLPCDIVNSYTKGNPPVQPKHSQMITNNSTSVTCWLDSWGDGGCGILYFMIESRVYGRSSWAVVSNHIPPTERIYTVSDLVPGTKYQLKVTAHNNAGSTTAIYNFTTLSTQGVIYNNDHSTPVSHLSDLPFYANFKLLLPICFSLLMLLALIGAALFLRKRKLASQARLASSSMSESPSLANLQNKQNRDQQYLAVRCNPGTSAPRGSNSNDSGSFGKAEGNEYIEDICPYATFQLNKQTYSESSYSGNVYSGPYHSVRGSFVYHDVKPESYHSKEPEYTKVRRKVGRLRDPHSESQEYDTLGSESDNDVSARALNSAKYRAQRDTQDETSSSSETTPTSMTRKSKPPFAARKGGKPGTSGKRHVRSSSGYSSHNEETTFSISNYPPNYQDHINPPARFSDANDKTKTQTSPRMRANQKLHREAFQINV

Target ORF information:

RefSeq Version	NM_001144436.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster down syndrome cell adhesion molecule 4, transcript variant D (Dscam4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144436.2

ORF Insert Sequence:

ATGGATTTAC ACAGCCTTTT TAAGGCCATC CTGATCCTAT ATGTCATAAG AGCCGAGACC 
AGCCAGTTTG TAAATGGACT GGATCTACAG GGACCGATTT TTCTCCATGA GCCACCGCAT 
CGCGTCGAGT TCTCCAACAA TTCGGGCGGA CTCATAGAAT GCTCCGGGCA TGGAAGTCCC 
CCGCCGGAGG TGGAGTGGAC CCCCATCCCG CCGCAGCAGG ACATGGTGTT CCAGCTCTCC 
AACGGTAGCA TGATGTTCTA TCCCTTTACG GCCGAGAAGT ACCGCCACGA GGTGCATGCC 
ACAGTCTACA GATGCAAACT GCGCAATTTG GTGGGCACCG TGCTCAGCCG AGAGGTTCAC 
GTGCGCGGCG TCGTCAACCA GAAGTACGCG GTCCAGGTGC ACGATGAATA CGTGATGACG 
GGCAATACGG CGGTGCTGAA ATGCCAGGTT CCCAGCTACA TGTCGGAATT CGTATTGGTC 
ACCGCTTGGG TGCAGGACAC CGGCATGCAT TTGTATCCGA ACACGGACAT CGGTGGCAAG 
TACACTGTGC TATCGAACGG CGAATTGTAC ATAAATAATG CGGGACCCAA TGATGCGTAC 
AAAAGCTACA CATGCCGCAC TGTAAATAGA CTGACAGGTG AAGTACAAAT TTCCACATAT 
CCCGGCCGCA TTATTGTAAC AGAGCCCAAG GGCATGGTAC AGCCGCGCAT CAACGTGGAG 
AAGCATTCGA TGCGGCATGT TGTCCTTAAT GGCCAAACAA CGTTGCCGTG CATAGCTCAA 
GGACATCCGG TGCCAACATA TCGGTGGTTC AAGGAGGAAA ACGAGCAACT GCTGCCGCTG 
CAACTCAGCG AACGCATCAC CATCGTATCC GCAGGACTCC TCAAAATCAC CAAGGCACGT 
CTCGAGGATA GTGGAAAATA TTTGTGCTGG GTGAACAATA CGGCTGGCGA GGAGACCATC 
CAAGTGTCGC TAACGGTGAC AGCTCCTTTG ACGGCGCACC TACAGCCACA GGTGCAGACG 
GTGGATGTCG ATAAGGATGC GCAATTCCAA TGCATTGTCT CTGGCCATCC GGTCCACGAT 
GTCAACTGGC TGCACGACGG CAAACCCATA CTCAGGGACA ATCGGGTTGA GATACTCACC 
GATCCACCGC GACTGATAAT TAAAAAAGTG CAAAAGGAGG ACCCTGGCAT GTACCAGTGC 
TTCGTCTCCA ACGAATGGGA GCAGATCCAA TCCACCGCTG AACTGCAGTT GGGCGATGCC 
TCGCCGGAAC TGCTTTATTG GTTCTCGGAG CAAACGCTGC AGCCAGGACC AACGGTATCA 
TTGAAGTGCG TAGCCACCGG AAATCCACTG CCTCAATTTA CATGGTCCCT GGACGGATTT 
CCGATACCAG ACAGCTCGCG CTTTTTAGTG GGTCAATATG TTACCATCCA CGACGATGTC 
ATCAGCCACG TGAATATATC AAATGTCAAG GAGGAGGACG GCGGGGAGTA CACCTGCACG 
GCCCAGAATG CAATTGGCAA AGTCTCGCAC AGCGCCAAAG TGAACATCTA TGGATTGCCT 
TACATACGCG AAATGCCAAA AATAACAGGC ATTTCTGGCT CTGATTTGAT TGTTAAATGT 
CCCGTGGCTG GTTACCCCAT CGATAAAATT CACTGGGAGC GAGATGGCCA AACGCTGCCC 
ATAAATCGCA GGCAGCGTGC CTACAACAAT GGCACCTTAA TTATCGAGCA ACTGCAGCGC 
CTCGAGGATG CGGGCACCTA CACGTGCATG GCGCAGAACA AACAAAAGCA AACATCCCGC 
CGGAATGTGG AGATCCAAGT GCTGGTGCCG CCGAAAATTA TGCCCATCCA AGCCATGACG 
AACATGCTCC GCGAGGGAAT GCGCGCCGCC ATATCCTGCC AAATCCTCGA GGGCGACCTG 
CCCGTCAGTT TTCGATGGGA GCGCAACGGA AAGCCACTGA TCGGAACGGG CAATGAGGTT 
TTCCGCCGCC TGGATGAGTA TTCCGCCAGT TTGGTCATCG AGCACATTTC CTCCGACCAT 
TCCGGAAACT ACACCTGCAT CGCGAGCAAT GTGGCAGGTA CGGAAAGATT CACCGTACCC 
CTGACCGTGA ATGTACCGCC CAAGTGGATC CTGGAGCCAA AGGACTCGAG TGCCCAGGCG 
GGTGCAGATG TCCTTCTGCA CTGTCAATCC TCAGGATATC CCACACCGAC AATCACGTGG 
AAAAAGGCGA TTGGACCAAC GCCAGGGGAG TACAAGGATT TCCTTTACGA GCCCACAGTG 
CAGCTATTTC CCAACGGAAC CATTTTCTTC AAGAAGATCA GCAAGGAGTC GCAAGGACAC 
TTCCTGTGCG AGGCCAAAAA TAACATTGGT TCCGGTGTCA GCAAAGTCAT CTTCCTCAAA 
GTGAATGTGC CCGCCCACTT TCAGACGAAG ACCAAACAGA TTTCGGTGGC CAAAGGAAAG 
CAGGTCCATG TGCAGTGCAA CGTGCAGGGC GACAATCCCA TCGACTTTAA ATGGAAAATC 
CAGGCAACGC AACAGTATCT CGACGAGTCG CTGGATTCCC GCTACACAAT AAGAGATCAA 
GTGCTCGACG ACGGCATGGT CTCCGAATTG GGCATTTCGC ACACATATCG CCAGGATACG 
GGCATTTATA TCTGTCAGGC GAGCAATGCA TTCGGACAGG ACGAGATGTC CATTCAGCTC 
ATCGTGCAGG AAGTGCCCGA GCAGCCGAAG AACCTTCGCA TCAACTCGCA ACAGTCGCGC 
AGCCTTCAGC TCACGTGGAG CCAACCCTTT GCCGGAAATA GTCCCATCGA GGAGTACCAC 
ATCTACTACA AACAAATATC AGATTTCTTT TCACCCTCAG ACATTTGGCA AAATGCGGAG 
CACCTCACCA TCGCCGGAGC CCAGACCGTG ATCAACATCC AGCAGCTGCG ACCGGCAAAA 
GCCTATCACA TCCGCATGTC GGCGGAGAAC AAGCTGGGCG CCTCCGAGTT CTCGGAGGTG 
GTGCAAGTGA CCACGCTGGA GGAGGTACCC TCGGGTCCAC CACTTGCCGT TCGGGCTGAG 
CCCAAGAGCT CCACCGAGAT CTTCGTCACG TGGGATGCAC CGGAACGGGA TCACTGGAAC 
GGCATCCTGC TCGGCTACTA CGTGGGCTAC CAGATGTCGC TGACGCCCGA GGACAAGGAG 
GTGAATCCCA CGCAGGGATT CAGCTTCAAA ACCGTCGAGG TGCGCTCTCA TTTCGGTGGC 
GAGACGGTGC TGGCCAATCT CAACAAGTTC ACCCAATACC ACGTGATTGT GCAGGCCTAC 
ACGAGCCAGG GTAGCGGACC ACCGAGCAAG GAGATTGCCG TGCAAACAAT GGAGGACGTT 
CCCTCATCGC CGCCTGAATC GCCGCAGTGC GATGTCCTGG GCTCCACTTC GATCTATATC 
ACCTGGTCAC CGCCGGACAT TGATGGCCAA AATGGAAAAA TCAAGGGCTA TAAAGTGTTT 
TACATATCGG TGGACGAGCT GTACGAAACC GATCCGGAGG TTGTCAAGTC AACAAATCAA 
TACGTCACCA TCGAGAATCT GCGCAAATAC ACCAACTACA CGGTCTGGGT TTTGGCTTAC 
ACCAAAGTAG GCGATGGCAT GAAAACCAAA CCGTTTTATT GCCGCACCCA CGAGGATGTG 
CCCTCGGCCC CGCAGGCCAT CAAGGCGATA CCCGCGTCGA GCTCGAAAAT CATAATATCC 
TGGCTGCCGC CCGACCTGCC CAATGGTGAC ATTACCGGCT ACACCTTCTA CATGTCCATG 
CTGGAGGGTG GACGCGAGGA GGGCACCCAC AAGCGCCTCC TAGGTCCCTT CGTGGAAATG 
CACGAAACGG TGCGCACCCA GGAGTCGGCC ACGTACCAGT TCTGGCTGAC TGCCTCCACC 
AAGATGGGCG AGGGCGAGAA GACGCAGGTG GTAACTGTGC CGCCGAACAA TAAGGTGCCC 
GCCCGCATAG TGTCCTTCAG CCAGCGAATA GTCACGCCCT GGAAGGAGCA TCTGGAGCTG 
CCATGCCGGA AGGTGGGAGC ACCTGCTCCG GTTACCATTT GGCGCCAGGA TGGTCACAAT 
ATGGAGACGA GTGCCCGCAA GACGATAGCC AAGAATGGCA CGCTCTACAT GAAGGAGTGC 
CAGGCGAGTG ATGCGGGCAA CTACACTTGC AGTGTGGAGA ACACCTGGGG CAAGGACGAG 
ATCGTGTACA ACATTGTGGT AAAGGTGCCA CCAGAGGCGC CCAATCTCAC GGTGATAAAT 
GCCTACACGG ACAGTCTGCT CCTCGAATGG ATGGACAATA GTCATGGTGG TAGTCCCATT 
CTGGGTTATG TGATCAATTA CAAGCGCGAC AACGGCGATT GGGAAGAGCT GCAGGTGGAC 
TCGAAGACCA CGTCGCACCT GCTGACCAAC CTGTGGTGTG GCACTCGCTA TCAGTTGTAC 
ATAACCGCTT ACAATAAGAT TGGAACGGGC TTACCCTGCG ACATAGTCAA CTCCTATACG 
AAGGGAAATC CGCCCGTGCA ACCGAAGCAC TCCCAGATGA TTACCAACAA CTCAACGAGC 
GTCACCTGCT GGCTGGATTC CTGGGGAGAT GGTGGCTGTG GCATCCTGTA CTTCATGATC 
GAGTCCAGGG TTTATGGCCG ATCCTCGTGG GCAGTGGTGT CCAATCACAT TCCGCCGACT 
GAGAGGATAT ACACGGTGAG CGATCTGGTG CCCGGCACAA AGTACCAACT GAAAGTCACG 
GCACACAATA ATGCCGGCTC CACCACTGCC ATCTACAACT TTACCACACT GTCTACACAA 
GGAGTGATTT ACAACAACGA TCACTCCACA CCTGTATCCC ACCTGAGCGA CCTGCCCTTC 
TATGCCAATT TCAAACTACT GCTGCCCATA TGCTTTTCCC TACTGATGTT GCTGGCTTTG 
ATTGGTGCCG CTCTATTCCT CAGGAAGCGA AAGCTAGCCA GCCAGGCCCG TCTGGCCAGT 
TCCTCGATGT CGGAGTCGCC ATCCCTGGCC AATCTGCAGA ACAAACAGAA TCGGGACCAG 
CAGTACCTAG CCGTGCGATG CAATCCGGGC ACATCTGCTC CGCGGGGATC CAACTCCAAT 
GATTCCGGCA GCTTTGGCAA GGCGGAGGGA AACGAGTACA TCGAGGACAT CTGCCCGTAT 
GCAACCTTTC AACTGAACAA ACAGACTTAT AGCGAGAGCT CCTACAGCGG CAATGTCTAC 
AGTGGGCCCT ACCACTCGGT GCGCGGATCC TTTGTCTACC ACGATGTCAA GCCGGAAAGC 
TACCACTCCA AGGAACCGGA GTACACCAAA GTGCGGCGAA AAGTGGGTCG CCTCAGGGAT 
CCTCATTCAG AGAGCCAAGA ATATGACACA CTCGGCTCCG AGTCCGACAA TGATGTGTCT 
GCGCGAGCTC TAAACTCGGC CAAGTATCGA GCACAACGTG ATACTCAGGA CGAGACTTCG 
TCCTCCTCGG AAACCACACC CACTAGCATG ACCCGGAAGT CAAAGCCGCC CTTTGCCGCC 
CGGAAGGGCG GAAAGCCCGG AACGAGCGGA AAGCGGCACG TCCGAAGTTC CAGTGGCTAT 
TCGAGCCACA ATGAAGAAAC TACTTTTAGC ATATCCAACT ATCCACCAAA CTATCAGGAC 
CACATAAATC CACCAGCTCG TTTTTCGGAT GCCAACGACA AGACGAAGAC CCAGACGTCG 
CCACGGATGC GTGCCAATCA GAAACTACAC CGGGAGGCTT TCCAGATAAA TGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dscam4 ( NM_001144437.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137909.1 Drosophila melanogaster down syndrome cell adhesion molecule 4, transcript variant H (Dscam4), mRNA.

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CloneID	OFa20094
Clone ID Related Accession (Same CDS sequence)	NM_001144437.2
Accession Version	NM_001144437.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	down syndrome cell adhesion molecule 4, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001144437.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTTAC ACAGCCTTTT TAAGGCCATC CTGATCCTAT ATGTCATAAG AGCCGAGACC 
AGCCAGTTTG TAAATGGACT GGATCTACAG GGACCGATTT TTCTCCATGA GCCACCGCAT 
CGCGTCGAGT TCTCCAACAA TTCGGGCGGA CTCATAGAAT GCTCCGGGCA TGGAAGTCCC 
CCGCCGGAGG TGGAGTGGAC CCCCATCCCG CCGCAGCAGG ACATGGTGTT CCAGCTCTCC 
AACGGTAGCA TGATGTTCTA TCCCTTTACG GCCGAGAAGT ACCGCCACGA GGTGCATGCC 
ACAGTCTACA GATGCAAACT GCGCAATTTG GTGGGCACCG TGCTCAGCCG AGAGGTTCAC 
GTGCGCGGCG TCGTCAACCA GAAGTACGCG GTCCAGGTGC ACGATGAATA CGTGATGACG 
GGCAATACGG CGGTGCTGAA ATGCCAGGTT CCCAGCTACA TGTCGGAATT CGTATTGGTC 
ACCGCTTGGG TGCAGGACAC CGGCATGCAT TTGTATCCGA ACACGGACAT CGGTGGCAAG 
TACACTGTGC TATCGAACGG CGAATTGTAC ATAAATAATG CGGGACCCAA TGATGCGTAC 
AAAAGCTACA CATGCCGCAC TGTAAATAGA CTGACAGGTG AAGTACAAAT TTCCACATAT 
CCCGGCCGCA TTATTGTAAC AGAGCCCAAG GGCATGGTAC AGCCGCGCAT CAACGTGGAG 
AAGCATTCGA TGCGGCATGT TGTCCTTAAT GGCCAAACAA CGTTGCCGTG CATAGCTCAA 
GGACATCCGG TGCCAACATA TCGGTGGTTC AAGGAGGAAA ACGAGCAACT GCTGCCGCTG 
CAACTCAGCG AACGCATCAC CATCGTATCC GCAGGACTCC TCAAAATCAC CAAGGCACGT 
CTCGAGGATA GTGGAAAATA TTTGTGCTGG GTGAACAATA CGGCTGGCGA GGAGACCATC 
CAAGTGTCGC TAACGGTGAC AGCTCCTTTG ACGGCGCACC TACAGCCACA GGTGCAGACG 
GTGGATGTCG ATAAGGATGC GCAATTCCAA TGCATTGTCT CTGGCCATCC GGTCCACGAT 
GTCAACTGGC TGCACGACGG CAAACCCATA CTCAGGGACA ATCGGGTTGA GATACTCACC 
GATCCACCGC GACTGATAAT TAAAAAAGTG CAAAAGGAGG ACCCTGGCAT GTACCAGTGC 
TTCGTCTCCA ACGAATGGGA GCAGATCCAA TCCACCGCTG AACTGCAGTT GGGCGATGCC 
TCGCCGGAAC TGCTTTATTG GTTCTCGGAG CAAACGCTGC AGCCAGGACC AACGGTATCA 
TTGAAGTGCG TAGCCACCGG AAATCCACTG CCTCAATTTA CATGGTCCCT GGACGGATTT 
CCGATACCAG ACAGCTCGCG CTTTTTAGTG GGTCAATATG TTACCATCCA CGACGATGTC 
ATCAGCCACG TGAATATATC AAATGTCAAG GAGGAGGACG GCGGGGAGTA CACCTGCACG 
GCCCAGAATG CAATTGGCAA AGTCTCGCAC AGCGCCAAAG TGAACATCTA TGGATTGCCT 
TACATACGCG AAATGCCAAA AATAACAGGC ATTTCTGGCT CTGATTTGAT TGTTAAATGT 
CCCGTGGCTG GTTACCCCAT CGATAAAATT CACTGGGAGC GAGATGGCCA AACGCTGCCC 
ATAAATCGCA GGCAGCGTGC CTACAACAAT GGCACCTTAA TTATCGAGCA ACTGCAGCGC 
CTCGAGGATG CGGGCACCTA CACGTGCATG GCGCAGAACA AACAAAAGCA AACATCCCGC 
CGGAATGTGG AGATCCAAGT GCTGGTGCCG CCGAAAATTA TGCCCATCCA AGCCATGACG 
AACATGCTCC GCGAGGGAAT GCGCGCCGCC ATATCCTGCC AAATCCTCGA GGGCGACCTG 
CCCGTCAGTT TTCGATGGGA GCGCAACGGA AAGCCACTGA TCGGAACGGG CAATGAGGTT 
TTCCGCCGCC TGGATGAGTA TTCCGCCAGT TTGGTCATCG AGCACATTTC CTCCGACCAT 
TCCGGAAACT ACACCTGCAT CGCGAGCAAT GTGGCAGGTA CGGAAAGATT CACCGTACCC 
CTGACCGTGA ATGTACCGCC CAAGTGGATC CTGGAGCCAA AGGACTCGAG TGCCCAGGCG 
GGTGCAGATG TCCTTCTGCA CTGTCAATCC TCAGGATATC CCACACCGAC AATCACGTGG 
AAAAAGGCGA TTGGACCAAC GCCAGGGGAG TACAAGGATT TCCTTTACGA GCCCACAGTG 
CAGCTATTTC CCAACGGAAC CATTTTCTTC AAGAAGATCA GCAAGGAGTC GCAAGGACAC 
TTCCTGTGCG AGGCCAAAAA TAACATTGGT TCCGGTGTCA GCAAAGTCAT CTTCCTCAAA 
GTGAATGTGC CCGCCCACTT TCAGACGAAG ACCAAACAGA TTTCGGTGGC CAAAGGAAAG 
CAGGTCCATG TGCAGTGCAA CGTGCAGGGC GACAATCCCA TCGACTTTAA ATGGAAAATC 
CAGGCAACGC AACAGTATCT CGACGAGTCG CTGGATTCCC GCTACACAAT AAGAGATCAA 
GTGCTCGACG ACGGCATGGT CTCCGAATTG GGCATTTCGC ACACATATCG CCAGGATACG 
GGCATTTATA TCTGTCAGGC GAGCAATGCA TTCGGACAGG ACGAGATGTC CATTCAGCTC 
ATCGTGCAGG AAGTGCCCGA GCAGCCGAAG AACCTTCGCA TCAACTCGCA ACAGTCGCGC 
AGCCTTCAGC TCACGTGGAG CCAACCCTTT GCCGGAAATA GTCCCATCGA GGAGTACCAC 
ATCTACTACA AACAAATATC AGATTTCTTT TCACCCTCAG ACATTTGGCA AAATGCGGAG 
CACCTCACCA TCGCCGGAGC CCAGACCGTG ATCAACATCC AGCAGCTGCG ACCGGCAAAA 
GCCTATCACA TCCGCATGTC GGCGGAGAAC AAGCTGGGCG CCTCCGAGTT CTCGGAGGTG 
GTGCAAGTGA CCACGCTGGA GGAGGTACCC TCGGGTCCAC CACTTGCCGT TCGGGCTGAG 
CCCAAGAGCT CCACCGAGAT CTTCGTCACG TGGGATGCAC CGGAACGGGA TCACTGGAAC 
GGCATCCTGC TCGGCTACTA CGTGGGCTAC CAGATGTCGC TGACGCCCGA GGACAAGGAG 
GTGAATCCCA CGCAGGGATT CAGCTTCAAA ACCGTCGAGG TGCGCTCTCA TTTCGGTGGC 
GAGACGGTGC TGGCCAATCT CAACAAGTTC ACCCAATACC ACGTGATTGT GCAGGCCTAC 
ACGAGCCAGG GTAGCGGACC ACCGAGCAAG GAGATTGCCG TGCAAACAAT GGAGGACGTT 
CCCTCATCGC CGCCTGAATC GCCGCAGTGC GATGTCCTGG GCTCCACTTC GATCTATATC 
ACCTGGTCAC CGCCGGACAT TGATGGCCAA AATGGAAAAA TCAAGGGCTA TAAAGTGTTT 
TACATATCGG TGGACGAGCT GTACGAAACC GATCCGGAGG TTGTCAAGTC AACAAATCAA 
TACGTCACCA TCGAGAATCT GCGCAAATAC ACCAACTACA CGGTCTGGGT TTTGGCTTAC 
ACCAAAGTAG GCGATGGCAT GAAAACCAAA CCGTTTTATT GCCGCACCCA CGAGGATGTG 
CCCTCGGCCC CGCAGGCCAT CAAGGCGATA CCCGCGTCGA GCTCGAAAAT CATAATATCC 
TGGCTGCCGC CCGACCTGCC CAATGGTGAC ATTACCGGCT ACACCTTCTA CATGTCCATG 
CTGGAGGGTG GACGCGAGGA GGGCACCCAC AAGCGCCTCC TAGGTCCCTT CGTGGAAATG 
CACGAAACGG TGCGCACCCA GGAGTCGGCC ACGTACCAGT TCTGGCTGAC TGCCTCCACC 
AAGATGGGCG AGGGCGAGAA GACGCAGGTG GTAACTGTGC CGCCGAACAA TAAGGTGCCC 
GCCCGCATAG TGTCCTTCAG CCAGCGAATA GTCACGCCCT GGAAGGAGCA TCTGGAGCTG 
CCATGCCGGA AGGTGGGAGC ACCTGCTCCG GTTACCATTT GGCGCCAGGA TGGTCACAAT 
ATGGAGACGA GTGCCCGCAA GACGATAGCC AAGAATGGCA CGCTCTACAT GAAGGAGTGC 
CAGGCGAGTG ATGCGGGCAA CTACACTTGC AGTGTGGAGA ACACCTGGGG CAAGGACGAG 
ATCGTGTACA ACATTGTGGT AAAGGTGCCA CCAGAGGCGC CCAATCTCAC GGTGATAAAT 
GCCTACACGG ACAGTCTGCT CCTCGAATGG ATGGACAATA GTCATGGTGG TAGTCCCATT 
CTGGGTTATG TGATCAATTA CAAGCGCGAC AACGGCGATT GGGAAGAGCT GCAGGTGGAC 
TCGAAGACCA CGTCGCACCT GCTGACCAAC CTGTGGTGTG GCACTCGCTA TCAGTTGTAC 
ATAACCGCTT ACAATAAGAT TGGAACGGGC TTACCCTGCG ACATAGTCAA CTCCTATACG 
AAGGGAAATC CGCCCGTGCA ACCGAAGCAC TCCCAGATGA TTACCAACAA CTCAACGAGC 
GTCACCTGCT GGCTGGATTC CTGGGGAGAT GGTGGCTGTG GCATCCTGTA CTTCATGATC 
GAGTCCAGGG TTTATGGCCG ATCCTCGTGG GCAGTGGTGT CCAATCACAT TCCGCCGACT 
GAGAGGATAT ACACGGTGAG CGATCTGGTG CCCGGCACAA AGTACCAACT GAAAGTCACG 
GCACACAATA ATGCCGGCTC CACCACTGCC ATCTACAACT TTACCACACT GTCTACACAA 
GGAGTGATTT ACAACAACGA TCACTCCACA CCTGTATCCC ACCTGAGCGA CCTGCCCTTC 
TATGCCAATT TCAAACTACT GCTGCCCATA TGCTTTTCCC TACTGATGTT GCTGGCTTTG 
ATTGGTGCCG CTCTATTCCT CAGGAAGCGA AAGCTAGCCA GCCAGGCCCG TCTGGCCAGT 
TCCTCGATGT CGGAGTCGCC ATCCCTGGCC AATCTGCAGA ACAAACAGAA TCGGGACCAG 
CAGTACCTAG CCGTGCGATG CAATCCGGGC ACATCTGCTC CGCGGGGATC CAACTCCAAT 
GATTCCGGCA GCTTTGGCAA GGCGGAGGGA AACGAGTACA TCGAGGACAT CTGCCCGTAT 
GCAACCTTTC AACTGAACAA ACAGACTTAT AGCGAGAGCT CCTACAGCGG CAATGTCTAC 
AGTGGGCCCT ACCACTCGGT GCGCGGATCC TTTGTCTACC ACGATGTCAA GCCGGAAAGC 
TACCACTCCA AGGAACCGGA GTACACCAAA GTGCGGCGAA AAGTGGGTCG CCTCAGGGAT 
CCTCATTCAG AGAGCCAAGA ATATGACACA CTCGGCTCCG AGTCCGACAA TGATGTGTCT 
GCGCGAGCTC TAAACTCGGC CAAGTATCGA GCACAACGTG ATACTCAGGA CGAGACTTCG 
TCCTCCTCGG AAACCACACC CACTAGCATG ACCCGGAAGT CAAAGCCGCC CTTTGCCGCC 
CGGAAGGGCG GAAAGCCCGG AACGAGCGGA AAGCGGCACG TCCGAAGTTC CAGTGGCTAT 
TCGAGCCACA ATGAAGAAAC TACTTTTAGA ACCCTGTACG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137909.1
CDS	517..6141
Translation	MDLHSLFKAILILYVIRAETSQFVNGLDLQGPIFLHEPPHRVEFSNNSGGLIECSGHGSPPPEVEWTPIPPQQDMVFQLSNGSMMFYPFTAEKYRHEVHATVYRCKLRNLVGTVLSREVHVRGVVNQKYAVQVHDEYVMTGNTAVLKCQVPSYMSEFVLVTAWVQDTGMHLYPNTDIGGKYTVLSNGELYINNAGPNDAYKSYTCRTVNRLTGEVQISTYPGRIIVTEPKGMVQPRINVEKHSMRHVVLNGQTTLPCIAQGHPVPTYRWFKEENEQLLPLQLSERITIVSAGLLKITKARLEDSGKYLCWVNNTAGEETIQVSLTVTAPLTAHLQPQVQTVDVDKDAQFQCIVSGHPVHDVNWLHDGKPILRDNRVEILTDPPRLIIKKVQKEDPGMYQCFVSNEWEQIQSTAELQLGDASPELLYWFSEQTLQPGPTVSLKCVATGNPLPQFTWSLDGFPIPDSSRFLVGQYVTIHDDVISHVNISNVKEEDGGEYTCTAQNAIGKVSHSAKVNIYGLPYIREMPKITGISGSDLIVKCPVAGYPIDKIHWERDGQTLPINRRQRAYNNGTLIIEQLQRLEDAGTYTCMAQNKQKQTSRRNVEIQVLVPPKIMPIQAMTNMLREGMRAAISCQILEGDLPVSFRWERNGKPLIGTGNEVFRRLDEYSASLVIEHISSDHSGNYTCIASNVAGTERFTVPLTVNVPPKWILEPKDSSAQAGADVLLHCQSSGYPTPTITWKKAIGPTPGEYKDFLYEPTVQLFPNGTIFFKKISKESQGHFLCEAKNNIGSGVSKVIFLKVNVPAHFQTKTKQISVAKGKQVHVQCNVQGDNPIDFKWKIQATQQYLDESLDSRYTIRDQVLDDGMVSELGISHTYRQDTGIYICQASNAFGQDEMSIQLIVQEVPEQPKNLRINSQQSRSLQLTWSQPFAGNSPIEEYHIYYKQISDFFSPSDIWQNAEHLTIAGAQTVINIQQLRPAKAYHIRMSAENKLGASEFSEVVQVTTLEEVPSGPPLAVRAEPKSSTEIFVTWDAPERDHWNGILLGYYVGYQMSLTPEDKEVNPTQGFSFKTVEVRSHFGGETVLANLNKFTQYHVIVQAYTSQGSGPPSKEIAVQTMEDVPSSPPESPQCDVLGSTSIYITWSPPDIDGQNGKIKGYKVFYISVDELYETDPEVVKSTNQYVTIENLRKYTNYTVWVLAYTKVGDGMKTKPFYCRTHEDVPSAPQAIKAIPASSSKIIISWLPPDLPNGDITGYTFYMSMLEGGREEGTHKRLLGPFVEMHETVRTQESATYQFWLTASTKMGEGEKTQVVTVPPNNKVPARIVSFSQRIVTPWKEHLELPCRKVGAPAPVTIWRQDGHNMETSARKTIAKNGTLYMKECQASDAGNYTCSVENTWGKDEIVYNIVVKVPPEAPNLTVINAYTDSLLLEWMDNSHGGSPILGYVINYKRDNGDWEELQVDSKTTSHLLTNLWCGTRYQLYITAYNKIGTGLPCDIVNSYTKGNPPVQPKHSQMITNNSTSVTCWLDSWGDGGCGILYFMIESRVYGRSSWAVVSNHIPPTERIYTVSDLVPGTKYQLKVTAHNNAGSTTAIYNFTTLSTQGVIYNNDHSTPVSHLSDLPFYANFKLLLPICFSLLMLLALIGAALFLRKRKLASQARLASSSMSESPSLANLQNKQNRDQQYLAVRCNPGTSAPRGSNSNDSGSFGKAEGNEYIEDICPYATFQLNKQTYSESSYSGNVYSGPYHSVRGSFVYHDVKPESYHSKEPEYTKVRRKVGRLRDPHSESQEYDTLGSESDNDVSARALNSAKYRAQRDTQDETSSSSETTPTSMTRKSKPPFAARKGGKPGTSGKRHVRSSSGYSSHNEETTFRTLYV

Target ORF information:

RefSeq Version	NM_001144437.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster down syndrome cell adhesion molecule 4, transcript variant H (Dscam4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144437.2

ORF Insert Sequence:

ATGGATTTAC ACAGCCTTTT TAAGGCCATC CTGATCCTAT ATGTCATAAG AGCCGAGACC 
AGCCAGTTTG TAAATGGACT GGATCTACAG GGACCGATTT TTCTCCATGA GCCACCGCAT 
CGCGTCGAGT TCTCCAACAA TTCGGGCGGA CTCATAGAAT GCTCCGGGCA TGGAAGTCCC 
CCGCCGGAGG TGGAGTGGAC CCCCATCCCG CCGCAGCAGG ACATGGTGTT CCAGCTCTCC 
AACGGTAGCA TGATGTTCTA TCCCTTTACG GCCGAGAAGT ACCGCCACGA GGTGCATGCC 
ACAGTCTACA GATGCAAACT GCGCAATTTG GTGGGCACCG TGCTCAGCCG AGAGGTTCAC 
GTGCGCGGCG TCGTCAACCA GAAGTACGCG GTCCAGGTGC ACGATGAATA CGTGATGACG 
GGCAATACGG CGGTGCTGAA ATGCCAGGTT CCCAGCTACA TGTCGGAATT CGTATTGGTC 
ACCGCTTGGG TGCAGGACAC CGGCATGCAT TTGTATCCGA ACACGGACAT CGGTGGCAAG 
TACACTGTGC TATCGAACGG CGAATTGTAC ATAAATAATG CGGGACCCAA TGATGCGTAC 
AAAAGCTACA CATGCCGCAC TGTAAATAGA CTGACAGGTG AAGTACAAAT TTCCACATAT 
CCCGGCCGCA TTATTGTAAC AGAGCCCAAG GGCATGGTAC AGCCGCGCAT CAACGTGGAG 
AAGCATTCGA TGCGGCATGT TGTCCTTAAT GGCCAAACAA CGTTGCCGTG CATAGCTCAA 
GGACATCCGG TGCCAACATA TCGGTGGTTC AAGGAGGAAA ACGAGCAACT GCTGCCGCTG 
CAACTCAGCG AACGCATCAC CATCGTATCC GCAGGACTCC TCAAAATCAC CAAGGCACGT 
CTCGAGGATA GTGGAAAATA TTTGTGCTGG GTGAACAATA CGGCTGGCGA GGAGACCATC 
CAAGTGTCGC TAACGGTGAC AGCTCCTTTG ACGGCGCACC TACAGCCACA GGTGCAGACG 
GTGGATGTCG ATAAGGATGC GCAATTCCAA TGCATTGTCT CTGGCCATCC GGTCCACGAT 
GTCAACTGGC TGCACGACGG CAAACCCATA CTCAGGGACA ATCGGGTTGA GATACTCACC 
GATCCACCGC GACTGATAAT TAAAAAAGTG CAAAAGGAGG ACCCTGGCAT GTACCAGTGC 
TTCGTCTCCA ACGAATGGGA GCAGATCCAA TCCACCGCTG AACTGCAGTT GGGCGATGCC 
TCGCCGGAAC TGCTTTATTG GTTCTCGGAG CAAACGCTGC AGCCAGGACC AACGGTATCA 
TTGAAGTGCG TAGCCACCGG AAATCCACTG CCTCAATTTA CATGGTCCCT GGACGGATTT 
CCGATACCAG ACAGCTCGCG CTTTTTAGTG GGTCAATATG TTACCATCCA CGACGATGTC 
ATCAGCCACG TGAATATATC AAATGTCAAG GAGGAGGACG GCGGGGAGTA CACCTGCACG 
GCCCAGAATG CAATTGGCAA AGTCTCGCAC AGCGCCAAAG TGAACATCTA TGGATTGCCT 
TACATACGCG AAATGCCAAA AATAACAGGC ATTTCTGGCT CTGATTTGAT TGTTAAATGT 
CCCGTGGCTG GTTACCCCAT CGATAAAATT CACTGGGAGC GAGATGGCCA AACGCTGCCC 
ATAAATCGCA GGCAGCGTGC CTACAACAAT GGCACCTTAA TTATCGAGCA ACTGCAGCGC 
CTCGAGGATG CGGGCACCTA CACGTGCATG GCGCAGAACA AACAAAAGCA AACATCCCGC 
CGGAATGTGG AGATCCAAGT GCTGGTGCCG CCGAAAATTA TGCCCATCCA AGCCATGACG 
AACATGCTCC GCGAGGGAAT GCGCGCCGCC ATATCCTGCC AAATCCTCGA GGGCGACCTG 
CCCGTCAGTT TTCGATGGGA GCGCAACGGA AAGCCACTGA TCGGAACGGG CAATGAGGTT 
TTCCGCCGCC TGGATGAGTA TTCCGCCAGT TTGGTCATCG AGCACATTTC CTCCGACCAT 
TCCGGAAACT ACACCTGCAT CGCGAGCAAT GTGGCAGGTA CGGAAAGATT CACCGTACCC 
CTGACCGTGA ATGTACCGCC CAAGTGGATC CTGGAGCCAA AGGACTCGAG TGCCCAGGCG 
GGTGCAGATG TCCTTCTGCA CTGTCAATCC TCAGGATATC CCACACCGAC AATCACGTGG 
AAAAAGGCGA TTGGACCAAC GCCAGGGGAG TACAAGGATT TCCTTTACGA GCCCACAGTG 
CAGCTATTTC CCAACGGAAC CATTTTCTTC AAGAAGATCA GCAAGGAGTC GCAAGGACAC 
TTCCTGTGCG AGGCCAAAAA TAACATTGGT TCCGGTGTCA GCAAAGTCAT CTTCCTCAAA 
GTGAATGTGC CCGCCCACTT TCAGACGAAG ACCAAACAGA TTTCGGTGGC CAAAGGAAAG 
CAGGTCCATG TGCAGTGCAA CGTGCAGGGC GACAATCCCA TCGACTTTAA ATGGAAAATC 
CAGGCAACGC AACAGTATCT CGACGAGTCG CTGGATTCCC GCTACACAAT AAGAGATCAA 
GTGCTCGACG ACGGCATGGT CTCCGAATTG GGCATTTCGC ACACATATCG CCAGGATACG 
GGCATTTATA TCTGTCAGGC GAGCAATGCA TTCGGACAGG ACGAGATGTC CATTCAGCTC 
ATCGTGCAGG AAGTGCCCGA GCAGCCGAAG AACCTTCGCA TCAACTCGCA ACAGTCGCGC 
AGCCTTCAGC TCACGTGGAG CCAACCCTTT GCCGGAAATA GTCCCATCGA GGAGTACCAC 
ATCTACTACA AACAAATATC AGATTTCTTT TCACCCTCAG ACATTTGGCA AAATGCGGAG 
CACCTCACCA TCGCCGGAGC CCAGACCGTG ATCAACATCC AGCAGCTGCG ACCGGCAAAA 
GCCTATCACA TCCGCATGTC GGCGGAGAAC AAGCTGGGCG CCTCCGAGTT CTCGGAGGTG 
GTGCAAGTGA CCACGCTGGA GGAGGTACCC TCGGGTCCAC CACTTGCCGT TCGGGCTGAG 
CCCAAGAGCT CCACCGAGAT CTTCGTCACG TGGGATGCAC CGGAACGGGA TCACTGGAAC 
GGCATCCTGC TCGGCTACTA CGTGGGCTAC CAGATGTCGC TGACGCCCGA GGACAAGGAG 
GTGAATCCCA CGCAGGGATT CAGCTTCAAA ACCGTCGAGG TGCGCTCTCA TTTCGGTGGC 
GAGACGGTGC TGGCCAATCT CAACAAGTTC ACCCAATACC ACGTGATTGT GCAGGCCTAC 
ACGAGCCAGG GTAGCGGACC ACCGAGCAAG GAGATTGCCG TGCAAACAAT GGAGGACGTT 
CCCTCATCGC CGCCTGAATC GCCGCAGTGC GATGTCCTGG GCTCCACTTC GATCTATATC 
ACCTGGTCAC CGCCGGACAT TGATGGCCAA AATGGAAAAA TCAAGGGCTA TAAAGTGTTT 
TACATATCGG TGGACGAGCT GTACGAAACC GATCCGGAGG TTGTCAAGTC AACAAATCAA 
TACGTCACCA TCGAGAATCT GCGCAAATAC ACCAACTACA CGGTCTGGGT TTTGGCTTAC 
ACCAAAGTAG GCGATGGCAT GAAAACCAAA CCGTTTTATT GCCGCACCCA CGAGGATGTG 
CCCTCGGCCC CGCAGGCCAT CAAGGCGATA CCCGCGTCGA GCTCGAAAAT CATAATATCC 
TGGCTGCCGC CCGACCTGCC CAATGGTGAC ATTACCGGCT ACACCTTCTA CATGTCCATG 
CTGGAGGGTG GACGCGAGGA GGGCACCCAC AAGCGCCTCC TAGGTCCCTT CGTGGAAATG 
CACGAAACGG TGCGCACCCA GGAGTCGGCC ACGTACCAGT TCTGGCTGAC TGCCTCCACC 
AAGATGGGCG AGGGCGAGAA GACGCAGGTG GTAACTGTGC CGCCGAACAA TAAGGTGCCC 
GCCCGCATAG TGTCCTTCAG CCAGCGAATA GTCACGCCCT GGAAGGAGCA TCTGGAGCTG 
CCATGCCGGA AGGTGGGAGC ACCTGCTCCG GTTACCATTT GGCGCCAGGA TGGTCACAAT 
ATGGAGACGA GTGCCCGCAA GACGATAGCC AAGAATGGCA CGCTCTACAT GAAGGAGTGC 
CAGGCGAGTG ATGCGGGCAA CTACACTTGC AGTGTGGAGA ACACCTGGGG CAAGGACGAG 
ATCGTGTACA ACATTGTGGT AAAGGTGCCA CCAGAGGCGC CCAATCTCAC GGTGATAAAT 
GCCTACACGG ACAGTCTGCT CCTCGAATGG ATGGACAATA GTCATGGTGG TAGTCCCATT 
CTGGGTTATG TGATCAATTA CAAGCGCGAC AACGGCGATT GGGAAGAGCT GCAGGTGGAC 
TCGAAGACCA CGTCGCACCT GCTGACCAAC CTGTGGTGTG GCACTCGCTA TCAGTTGTAC 
ATAACCGCTT ACAATAAGAT TGGAACGGGC TTACCCTGCG ACATAGTCAA CTCCTATACG 
AAGGGAAATC CGCCCGTGCA ACCGAAGCAC TCCCAGATGA TTACCAACAA CTCAACGAGC 
GTCACCTGCT GGCTGGATTC CTGGGGAGAT GGTGGCTGTG GCATCCTGTA CTTCATGATC 
GAGTCCAGGG TTTATGGCCG ATCCTCGTGG GCAGTGGTGT CCAATCACAT TCCGCCGACT 
GAGAGGATAT ACACGGTGAG CGATCTGGTG CCCGGCACAA AGTACCAACT GAAAGTCACG 
GCACACAATA ATGCCGGCTC CACCACTGCC ATCTACAACT TTACCACACT GTCTACACAA 
GGAGTGATTT ACAACAACGA TCACTCCACA CCTGTATCCC ACCTGAGCGA CCTGCCCTTC 
TATGCCAATT TCAAACTACT GCTGCCCATA TGCTTTTCCC TACTGATGTT GCTGGCTTTG 
ATTGGTGCCG CTCTATTCCT CAGGAAGCGA AAGCTAGCCA GCCAGGCCCG TCTGGCCAGT 
TCCTCGATGT CGGAGTCGCC ATCCCTGGCC AATCTGCAGA ACAAACAGAA TCGGGACCAG 
CAGTACCTAG CCGTGCGATG CAATCCGGGC ACATCTGCTC CGCGGGGATC CAACTCCAAT 
GATTCCGGCA GCTTTGGCAA GGCGGAGGGA AACGAGTACA TCGAGGACAT CTGCCCGTAT 
GCAACCTTTC AACTGAACAA ACAGACTTAT AGCGAGAGCT CCTACAGCGG CAATGTCTAC 
AGTGGGCCCT ACCACTCGGT GCGCGGATCC TTTGTCTACC ACGATGTCAA GCCGGAAAGC 
TACCACTCCA AGGAACCGGA GTACACCAAA GTGCGGCGAA AAGTGGGTCG CCTCAGGGAT 
CCTCATTCAG AGAGCCAAGA ATATGACACA CTCGGCTCCG AGTCCGACAA TGATGTGTCT 
GCGCGAGCTC TAAACTCGGC CAAGTATCGA GCACAACGTG ATACTCAGGA CGAGACTTCG 
TCCTCCTCGG AAACCACACC CACTAGCATG ACCCGGAAGT CAAAGCCGCC CTTTGCCGCC 
CGGAAGGGCG GAAAGCCCGG AACGAGCGGA AAGCGGCACG TCCGAAGTTC CAGTGGCTAT 
TCGAGCCACA ATGAAGAAAC TACTTTTAGA ACCCTGTACG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
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Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dscam4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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