ISPD cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ISPD
Entrez Gene ID	798716
Full Name	isoprenoid synthase domain containing
Synonyms	sb:eu371,zgc:154151
General protein information	Preferred Names isoprenoid synthase domain containing Names D-ribitol-5-phosphate cytidylyltransferase isoprenoid synthase domain-containing protein 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like protein notch1-induced protein
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005157764.3	XP_005157821.1	isoprenoid synthase domain-containing protein isoform X1
XM_017351046.2	XP_017206535.1	isoprenoid synthase domain-containing protein isoform X2
XM_009291808.3	XP_009290083.1	isoprenoid synthase domain-containing protein isoform X3
XM_005157764.4	XP_005157821.1	isoprenoid synthase domain-containing protein isoform X1
NM_001077802.1	NP_001071270.1	D-ribitol-5-phosphate cytidylyltransferase
XM_009291808.2	XP_009290083.1	isoprenoid synthase domain-containing protein isoform X3
XM_017351046.1	XP_017206535.1	isoprenoid synthase domain-containing protein isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ISPD	XP_002686725.1
Macaca mulatta (Rhesus monkey)	ISPD	XP_001105001.2
Mus musculus (house mouse)	Ispd	NP_848744.2
Gallus gallus (chicken)	ISPD	XP_004939388.1
Danio rerio (zebrafish)	ispd	NP_001071270.1
Homo sapiens (human)	ISPD	NP_001094896.1
Pan troglodytes (chimpanzee)	ISPD	XP_003318374.1
Rattus norvegicus (Norway rat)	Ispd	NP_001008387.1
Xenopus tropicalis (tropical clawed frog)	ispd	NP_001016240.1
Canis lupus familiaris (dog)	ISPD	XP_003431914.1

Gene Ontology
Bibliography

Related articles in PubMed

A limb-girdle muscular dystrophy 2I model of muscular dystrophy identifies corrective drug compounds for dystroglycanopathies.
Serafini PR, Feyder MJ, Hightower RM, Garcia-Perez D, Vieira NM, Lek A, Gibbs DE, Moukha-Chafiq O, Augelli-Szafran CE, Kawahara G, Widrick JJ, Kunkel LM, Alexander MS
JCI insight3(18)(2018 09)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Mutations in ISPD cause Walker-Warburg syndrome and defective glycosylation of α-dystroglycan.
Roscioli T, Kamsteeg EJ, Buysse K, Maystadt I, van Reeuwijk J, van den Elzen C, van Beusekom E, Riemersma M, Pfundt R, Vissers LE, Schraders M, Altunoglu U, Buckley MF, Brunner HG, Grisart B, Zhou H, Veltman JA, Gilissen C, Mancini GM, Delrée P, Willemsen MA, Ramadža DP, Chitayat D, Bennett C, Sheridan E, Peeters EA, Tan-Sindhunata GM, de Die-Smulders CE, Devriendt K, Kayserili H, El-Hashash OA, Stemple DL, Lefeber DJ, Lin YY, van Bokhoven H
Nature genetics44(5)581-5(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ISPD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ISPD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa38282	NM_001077802.1 Latest version!	Danio rerio CDP-L-ribitol pyrophosphorylase A (crppa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa20721	XM_005157764.4 Latest version!	Danio rerio isoprenoid synthase domain containing (ispd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa47290	XM_017351046.2 Latest version!	Danio rerio isoprenoid synthase domain containing (ispd), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ODa47291	XM_009291808.3 Latest version!	Danio rerio isoprenoid synthase domain containing (ispd), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
ODa20721	XM_005157764.3	Danio rerio isoprenoid synthase domain containing (ispd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa47291	XM_009291808.2	Danio rerio isoprenoid synthase domain containing (ispd), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ODa47290	XM_017351046.1	Danio rerio isoprenoid synthase domain containing (ispd), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ISPD ( NM_001077802.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001071270.1 Danio rerio CDP-L-ribitol pyrophosphorylase A (crppa), mRNA.

Price & Availability↑

CloneID	ODa38282
Clone ID Related Accession (Same CDS sequence)	NM_001077802.1
Accession Version	NM_001077802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Danio rerio(zebrafish)
Product	D-ribitol-5-phosphate cytidylyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC127405.1. On Dec 20, 2007 this sequence version replaced XM_001339114.1. ##Evidence-Data-START## Transcript exon combination :: BC127405.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGGAACGG TAACAATTCA GTTGTCTTGT CTCCGTCACA TTCGCAAACT TTGCTTTTCT 
TGTCCATGGG AGGGCAGACG TCTCTTCAAA ATGCTGTTAC AGTTCCACCA CGAGACGCAA 
AGGCCCTTGG ACCCCGGGTG TCTCCTATCA CAAGACGCCG AGCGGAGCGG CGACCAACCC 
GGGCGCGCGG TGGTGGATTT CCCGGTAGCT GCCGTTCTGC CCGCCGGAGG CTCCGGGGAG 
AGGATGGGAC TGACCACACC GAAACAATTC TGCTCCATCT TTAACAGACC TCTGATAAGC 
TACACCATTC AAGCATTTGA GAGATTACCG TGGATTGTAA TGGTTGTCGT TGTGATTGCT 
AAGGATAATC ATGACCTGAT GCTGAATATT GTCCGAAAAT TTAATCACAC AAAGGTAAAA 
GTAGTCCACG GGGGTACCAC CAGACACCGG TCCATCTACA ACGGTCTTCA GGCCTTCAGC 
GACTCTACAG ACTCATCGAC ACCCAAACCA AAAGTGGTCA TCATACATGA TGCAGTACGG 
CCATTTGTGG AAGAAGATCT TCTGCTGAAA ATCACCCTAG CAGCTAAAGA ACAAGGGGCC 
TCAGGAGCGA TTCGTCCTCT TGTGTCAACG GTCATTGCAA CCACGTCAGA AAGTTACCTG 
GACCACTCGC TGGAGAGAGC CAAGTACCGG GCCAGTGAAA TGCCTCAAGG ATTTCTTTAT 
GACATTATTT TCCAAGCCTA CCAGCGGTGC AGTGAATTTG ACTTAGAGTT TGGCACCGAG 
TGTCTACATT TGGCCCTGCA GTACTGTGGC ACGAATGCCA GGCTCATAGA GGGACCACCG 
ACTCTGTGGA AAGTAACATA CAAGCGAGAT CTAGCAGCTG CAGAGGCTAT TATTAAAGAG 
ACTCTTTCGG TGTCAGCCTG TATCATCGCA GAAGCTGAAG AAGAAGCTGT TGAGTTGGCC 
AAAACCCTTC AGAAAAATCT GAACATGATG GAGACAGACG TCATTCCATG TGGAAAGGAG 
AGCAATGTGC AGTACCTGTC CAAAACCCGA AACTTTATTC ACATATCTGC GAGTGCATCA 
AGCTCCTCGT GGGTGCTGGA AATGGTGAAA TGTTTTGAAG ACATCGACCA TGCTCGTTTA 
TACCCAGTAG TTATTGTTTG GGTACAGCTG AGCAAGACAA AGCAGTCGGC TGACAGCCAA 
GAAACAGACG AGTTCATGGC TCTGGCCTCA GAAGTCAAAC AGCGAAACGT TCTGCTCTAT 
GGAATTAAGA TCGATCACTC AAAGGAGCTG GAGCAATGGC AGAGATCACT GGAGAGACTC 
GGTCAGATCA CACTCGTCCT CATCAGAGAC AGAAACATGG CTCTCACTGG ACAGATGCTT 
CATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071270.1
CDS	26..1414
Translation	MGTVTIQLSCLRHIRKLCFSCPWEGRRLFKMLLQFHHETQRPLDPGCLLSQDAERSGDQPGRAVVDFPVAAVLPAGGSGERMGLTTPKQFCSIFNRPLISYTIQAFERLPWIVMVVVVIAKDNHDLMLNIVRKFNHTKVKVVHGGTTRHRSIYNGLQAFSDSTDSSTPKPKVVIIHDAVRPFVEEDLLLKITLAAKEQGASGAIRPLVSTVIATTSESYLDHSLERAKYRASEMPQGFLYDIIFQAYQRCSEFDLEFGTECLHLALQYCGTNARLIEGPPTLWKVTYKRDLAAAEAIIKETLSVSACIIAEAEEEAVELAKTLQKNLNMMETDVIPCGKESNVQYLSKTRNFIHISASASSSSWVLEMVKCFEDIDHARLYPVVIVWVQLSKTKQSADSQETDEFMALASEVKQRNVLLYGIKIDHSKELEQWQRSLERLGQITLVLIRDRNMALTGQMLHV

Target ORF information:

RefSeq Version	NM_001077802.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio CDP-L-ribitol pyrophosphorylase A (crppa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077802.1

ORF Insert Sequence:

ATGGGAACGG TAACAATTCA GTTGTCTTGT CTCCGTCACA TTCGCAAACT TTGCTTTTCT 
TGTCCATGGG AGGGCAGACG TCTCTTCAAA ATGCTGTTAC AGTTCCACCA CGAGACGCAA 
AGGCCCTTGG ACCCCGGGTG TCTCCTATCA CAAGACGCCG AGCGGAGCGG CGACCAACCC 
GGGCGCGCGG TGGTGGATTT CCCGGTAGCT GCCGTTCTGC CCGCCGGAGG CTCCGGGGAG 
AGGATGGGAC TGACCACACC GAAACAATTC TGCTCCATCT TTAACAGACC TCTGATAAGC 
TACACCATTC AAGCATTTGA GAGATTACCG TGGATTGTAA TGGTTGTCGT TGTGATTGCT 
AAGGATAATC ATGACCTGAT GCTGAATATT GTCCGAAAAT TTAATCACAC AAAGGTAAAA 
GTAGTCCACG GGGGTACCAC CAGACACCGG TCCATCTACA ACGGTCTTCA GGCCTTCAGC 
GACTCTACAG ACTCATCGAC ACCCAAACCA AAAGTGGTCA TCATACATGA TGCAGTACGG 
CCATTTGTGG AAGAAGATCT TCTGCTGAAA ATCACCCTAG CAGCTAAAGA ACAAGGGGCC 
TCAGGAGCGA TTCGTCCTCT TGTGTCAACG GTCATTGCAA CCACGTCAGA AAGTTACCTG 
GACCACTCGC TGGAGAGAGC CAAGTACCGG GCCAGTGAAA TGCCTCAAGG ATTTCTTTAT 
GACATTATTT TCCAAGCCTA CCAGCGGTGC AGTGAATTTG ACTTAGAGTT TGGCACCGAG 
TGTCTACATT TGGCCCTGCA GTACTGTGGC ACGAATGCCA GGCTCATAGA GGGACCACCG 
ACTCTGTGGA AAGTAACATA CAAGCGAGAT CTAGCAGCTG CAGAGGCTAT TATTAAAGAG 
ACTCTTTCGG TGTCAGCCTG TATCATCGCA GAAGCTGAAG AAGAAGCTGT TGAGTTGGCC 
AAAACCCTTC AGAAAAATCT GAACATGATG GAGACAGACG TCATTCCATG TGGAAAGGAG 
AGCAATGTGC AGTACCTGTC CAAAACCCGA AACTTTATTC ACATATCTGC GAGTGCATCA 
AGCTCCTCGT GGGTGCTGGA AATGGTGAAA TGTTTTGAAG ACATCGACCA TGCTCGTTTA 
TACCCAGTAG TTATTGTTTG GGTACAGCTG AGCAAGACAA AGCAGTCGGC TGACAGCCAA 
GAAACAGACG AGTTCATGGC TCTGGCCTCA GAAGTCAAAC AGCGAAACGT TCTGCTCTAT 
GGAATTAAGA TCGATCACTC AAAGGAGCTG GAGCAATGGC AGAGATCACT GGAGAGACTC 
GGTCAGATCA CACTCGTCCT CATCAGAGAC AGAAACATGG CTCTCACTGG ACAGATGCTT 
CATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISPD ( XM_005157764.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005157821.1 Danio rerio isoprenoid synthase domain containing (ispd), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20721
Clone ID Related Accession (Same CDS sequence)	XM_005157764.3 , XM_005157764.4
Accession Version	XM_005157764.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	isoprenoid synthase domain-containing protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157764.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATACGG CTGCCACAAA TGCGGAGTTA ATTGAAACCA AGGAAAAAAT GCAGCAAGAG 
CGCTGTTCTT CCTCCGACAG GCCCTTGGAC CCCGGGTGTC TCCTACCACA AGACGCCGAG 
CGGAGCGCCG ACCAACCCGG GCGCGCGGTG GTGGATTTCC CGGTAGCTGC CGTTCTGCCC 
GCCGGAGGCT CCGGGGAGAG GATGGGACTG ACCACACCGA AACAATTCTG CTCCATCTTT 
AACAGACCTC TGATAAGCTA CACCATTCAA GCATTTGAGA GATTACCGTG GATCGTAATG 
GTTGTCGTTG TGGTTGCTAA GGATAATCAT GACCTGATGC TGAATATTGT CCGAAAATTT 
AATCACACAA AGGTAAAAGT AGTCCACGGG GGTACCACCA GACACCGGTC CATCTACAAC 
GGTCTTCAGG CCTTCAGCGA CTCTACAGAC TCATCGACAC CCAAACCAAA AGTGGTCATC 
ATACATGATG CAGTACGGCC ATTTGTGGAA GAAGATCTTC TGCTGAAAAT CACCCTAGCA 
GCTAAAGAAC AAGGGGCCTC AGGAGCGATT CGTCCTCTTG TGTCAACGGT CATTGCAACC 
ACGTCAGAAA GTTACCTGGA CCACTCGCTG GAGAGAGCCA AGTACCGGGC CAGTGAAATG 
CCTCAAGGAT TTCTTTATGA CATTATTTTC CAAGCCTACC AGCGGTGCAG TGAATTTGAC 
TTAGAGTTTG GCACCGAGTG TCTACATTTG GCCCTGCAGT ACTGTGGCAC GAATGCCAGG 
CTCATAGAGG GACCACCGAC TCTGTGGAAA GTAACATACA AGCGAGATCT AGCAGCTGCA 
GAGGCTATTA TTAAAGAGAC TCTTTCGGTG TCAGCCTGTA TCATCGCAGA AGCTGAAGAA 
GAAGCTGTTG AGTTGGCCAA AACCCTTCAG AAAAATCTGA ACATGATGGA GACAGACGTC 
ATTCCATGTG GAAAGGAGAG CAATGTGCAG TACCTGTCCA AAACCCGAAA CTTTATTCAC 
ATATCTGCGA GTGCATCAAG CTCCTTGTGG GTGCTGGAAA TGGTGAAATG TTTTGAAGAC 
ATCGACCATG CTCGTTTATA CCCAGTAGTT ATTGTTTGGG TACAGCTGAG CATGACAAAG 
CAGTCGGCTG ACAGCCAAGA AACAGACGAG TTCATGGCTC TGGCCTCAGA AGTCAAACAG 
CGAAACGTTC TGCTCTATGG AATTAAGATC GATCACTCAA AGGAGCTGGA GCAATGGCAG 
AGATCACTGG AGAGACTCGG TCAGATCACA CTCGTCCTCA TCAGAGACAG AAACATGGCT 
CTCACTGGAC AGATGCTTCA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157821.1
CDS	320..1666
Translation	MNTAATNAELIETKEKMQQERCSSSDRPLDPGCLLPQDAERSADQPGRAVVDFPVAAVLPAGGSGERMGLTTPKQFCSIFNRPLISYTIQAFERLPWIVMVVVVVAKDNHDLMLNIVRKFNHTKVKVVHGGTTRHRSIYNGLQAFSDSTDSSTPKPKVVIIHDAVRPFVEEDLLLKITLAAKEQGASGAIRPLVSTVIATTSESYLDHSLERAKYRASEMPQGFLYDIIFQAYQRCSEFDLEFGTECLHLALQYCGTNARLIEGPPTLWKVTYKRDLAAAEAIIKETLSVSACIIAEAEEEAVELAKTLQKNLNMMETDVIPCGKESNVQYLSKTRNFIHISASASSSLWVLEMVKCFEDIDHARLYPVVIVWVQLSMTKQSADSQETDEFMALASEVKQRNVLLYGIKIDHSKELEQWQRSLERLGQITLVLIRDRNMALTGQMLHV

Target ORF information:

RefSeq Version	XM_005157764.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio isoprenoid synthase domain containing (ispd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157764.3

ORF Insert Sequence:

ATGAATACGG CTGCCACAAA TGCGGAGTTA ATTGAAACCA AGGAAAAAAT GCAGCAAGAG 
CGCTGTTCTT CCTCCGACAG GCCCTTGGAC CCCGGGTGTC TCCTACCACA AGACGCCGAG 
CGGAGCGCCG ACCAACCCGG GCGCGCGGTG GTGGATTTCC CGGTAGCTGC CGTTCTGCCC 
GCCGGAGGCT CCGGGGAGAG GATGGGACTG ACCACACCGA AACAATTCTG CTCCATCTTT 
AACAGACCTC TGATAAGCTA CACCATTCAA GCATTTGAGA GATTACCGTG GATCGTAATG 
GTTGTCGTTG TGGTTGCTAA GGATAATCAT GACCTGATGC TGAATATTGT CCGAAAATTT 
AATCACACAA AGGTAAAAGT AGTCCACGGG GGTACCACCA GACACCGGTC CATCTACAAC 
GGTCTTCAGG CCTTCAGCGA CTCTACAGAC TCATCGACAC CCAAACCAAA AGTGGTCATC 
ATACATGATG CAGTACGGCC ATTTGTGGAA GAAGATCTTC TGCTGAAAAT CACCCTAGCA 
GCTAAAGAAC AAGGGGCCTC AGGAGCGATT CGTCCTCTTG TGTCAACGGT CATTGCAACC 
ACGTCAGAAA GTTACCTGGA CCACTCGCTG GAGAGAGCCA AGTACCGGGC CAGTGAAATG 
CCTCAAGGAT TTCTTTATGA CATTATTTTC CAAGCCTACC AGCGGTGCAG TGAATTTGAC 
TTAGAGTTTG GCACCGAGTG TCTACATTTG GCCCTGCAGT ACTGTGGCAC GAATGCCAGG 
CTCATAGAGG GACCACCGAC TCTGTGGAAA GTAACATACA AGCGAGATCT AGCAGCTGCA 
GAGGCTATTA TTAAAGAGAC TCTTTCGGTG TCAGCCTGTA TCATCGCAGA AGCTGAAGAA 
GAAGCTGTTG AGTTGGCCAA AACCCTTCAG AAAAATCTGA ACATGATGGA GACAGACGTC 
ATTCCATGTG GAAAGGAGAG CAATGTGCAG TACCTGTCCA AAACCCGAAA CTTTATTCAC 
ATATCTGCGA GTGCATCAAG CTCCTTGTGG GTGCTGGAAA TGGTGAAATG TTTTGAAGAC 
ATCGACCATG CTCGTTTATA CCCAGTAGTT ATTGTTTGGG TACAGCTGAG CATGACAAAG 
CAGTCGGCTG ACAGCCAAGA AACAGACGAG TTCATGGCTC TGGCCTCAGA AGTCAAACAG 
CGAAACGTTC TGCTCTATGG AATTAAGATC GATCACTCAA AGGAGCTGGA GCAATGGCAG 
AGATCACTGG AGAGACTCGG TCAGATCACA CTCGTCCTCA TCAGAGACAG AAACATGGCT 
CTCACTGGAC AGATGCTTCA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISPD ( XM_005157764.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005157821.1 Danio rerio isoprenoid synthase domain containing (ispd), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20721
Clone ID Related Accession (Same CDS sequence)	XM_005157764.3 , XM_005157764.4
Accession Version	XM_005157764.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	isoprenoid synthase domain-containing protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157764.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATACGG CTGCCACAAA TGCGGAGTTA ATTGAAACCA AGGAAAAAAT GCAGCAAGAG 
CGCTGTTCTT CCTCCGACAG GCCCTTGGAC CCCGGGTGTC TCCTACCACA AGACGCCGAG 
CGGAGCGCCG ACCAACCCGG GCGCGCGGTG GTGGATTTCC CGGTAGCTGC CGTTCTGCCC 
GCCGGAGGCT CCGGGGAGAG GATGGGACTG ACCACACCGA AACAATTCTG CTCCATCTTT 
AACAGACCTC TGATAAGCTA CACCATTCAA GCATTTGAGA GATTACCGTG GATCGTAATG 
GTTGTCGTTG TGGTTGCTAA GGATAATCAT GACCTGATGC TGAATATTGT CCGAAAATTT 
AATCACACAA AGGTAAAAGT AGTCCACGGG GGTACCACCA GACACCGGTC CATCTACAAC 
GGTCTTCAGG CCTTCAGCGA CTCTACAGAC TCATCGACAC CCAAACCAAA AGTGGTCATC 
ATACATGATG CAGTACGGCC ATTTGTGGAA GAAGATCTTC TGCTGAAAAT CACCCTAGCA 
GCTAAAGAAC AAGGGGCCTC AGGAGCGATT CGTCCTCTTG TGTCAACGGT CATTGCAACC 
ACGTCAGAAA GTTACCTGGA CCACTCGCTG GAGAGAGCCA AGTACCGGGC CAGTGAAATG 
CCTCAAGGAT TTCTTTATGA CATTATTTTC CAAGCCTACC AGCGGTGCAG TGAATTTGAC 
TTAGAGTTTG GCACCGAGTG TCTACATTTG GCCCTGCAGT ACTGTGGCAC GAATGCCAGG 
CTCATAGAGG GACCACCGAC TCTGTGGAAA GTAACATACA AGCGAGATCT AGCAGCTGCA 
GAGGCTATTA TTAAAGAGAC TCTTTCGGTG TCAGCCTGTA TCATCGCAGA AGCTGAAGAA 
GAAGCTGTTG AGTTGGCCAA AACCCTTCAG AAAAATCTGA ACATGATGGA GACAGACGTC 
ATTCCATGTG GAAAGGAGAG CAATGTGCAG TACCTGTCCA AAACCCGAAA CTTTATTCAC 
ATATCTGCGA GTGCATCAAG CTCCTTGTGG GTGCTGGAAA TGGTGAAATG TTTTGAAGAC 
ATCGACCATG CTCGTTTATA CCCAGTAGTT ATTGTTTGGG TACAGCTGAG CATGACAAAG 
CAGTCGGCTG ACAGCCAAGA AACAGACGAG TTCATGGCTC TGGCCTCAGA AGTCAAACAG 
CGAAACGTTC TGCTCTATGG AATTAAGATC GATCACTCAA AGGAGCTGGA GCAATGGCAG 
AGATCACTGG AGAGACTCGG TCAGATCACA CTCGTCCTCA TCAGAGACAG AAACATGGCT 
CTCACTGGAC AGATGCTTCA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157821.1
CDS	310..1656
Translation	MNTAATNAELIETKEKMQQERCSSSDRPLDPGCLLPQDAERSADQPGRAVVDFPVAAVLPAGGSGERMGLTTPKQFCSIFNRPLISYTIQAFERLPWIVMVVVVVAKDNHDLMLNIVRKFNHTKVKVVHGGTTRHRSIYNGLQAFSDSTDSSTPKPKVVIIHDAVRPFVEEDLLLKITLAAKEQGASGAIRPLVSTVIATTSESYLDHSLERAKYRASEMPQGFLYDIIFQAYQRCSEFDLEFGTECLHLALQYCGTNARLIEGPPTLWKVTYKRDLAAAEAIIKETLSVSACIIAEAEEEAVELAKTLQKNLNMMETDVIPCGKESNVQYLSKTRNFIHISASASSSLWVLEMVKCFEDIDHARLYPVVIVWVQLSMTKQSADSQETDEFMALASEVKQRNVLLYGIKIDHSKELEQWQRSLERLGQITLVLIRDRNMALTGQMLHV

Target ORF information:

RefSeq Version	XM_005157764.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio isoprenoid synthase domain containing (ispd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157764.4

ORF Insert Sequence:

ATGAATACGG CTGCCACAAA TGCGGAGTTA ATTGAAACCA AGGAAAAAAT GCAGCAAGAG 
CGCTGTTCTT CCTCCGACAG GCCCTTGGAC CCCGGGTGTC TCCTACCACA AGACGCCGAG 
CGGAGCGCCG ACCAACCCGG GCGCGCGGTG GTGGATTTCC CGGTAGCTGC CGTTCTGCCC 
GCCGGAGGCT CCGGGGAGAG GATGGGACTG ACCACACCGA AACAATTCTG CTCCATCTTT 
AACAGACCTC TGATAAGCTA CACCATTCAA GCATTTGAGA GATTACCGTG GATCGTAATG 
GTTGTCGTTG TGGTTGCTAA GGATAATCAT GACCTGATGC TGAATATTGT CCGAAAATTT 
AATCACACAA AGGTAAAAGT AGTCCACGGG GGTACCACCA GACACCGGTC CATCTACAAC 
GGTCTTCAGG CCTTCAGCGA CTCTACAGAC TCATCGACAC CCAAACCAAA AGTGGTCATC 
ATACATGATG CAGTACGGCC ATTTGTGGAA GAAGATCTTC TGCTGAAAAT CACCCTAGCA 
GCTAAAGAAC AAGGGGCCTC AGGAGCGATT CGTCCTCTTG TGTCAACGGT CATTGCAACC 
ACGTCAGAAA GTTACCTGGA CCACTCGCTG GAGAGAGCCA AGTACCGGGC CAGTGAAATG 
CCTCAAGGAT TTCTTTATGA CATTATTTTC CAAGCCTACC AGCGGTGCAG TGAATTTGAC 
TTAGAGTTTG GCACCGAGTG TCTACATTTG GCCCTGCAGT ACTGTGGCAC GAATGCCAGG 
CTCATAGAGG GACCACCGAC TCTGTGGAAA GTAACATACA AGCGAGATCT AGCAGCTGCA 
GAGGCTATTA TTAAAGAGAC TCTTTCGGTG TCAGCCTGTA TCATCGCAGA AGCTGAAGAA 
GAAGCTGTTG AGTTGGCCAA AACCCTTCAG AAAAATCTGA ACATGATGGA GACAGACGTC 
ATTCCATGTG GAAAGGAGAG CAATGTGCAG TACCTGTCCA AAACCCGAAA CTTTATTCAC 
ATATCTGCGA GTGCATCAAG CTCCTTGTGG GTGCTGGAAA TGGTGAAATG TTTTGAAGAC 
ATCGACCATG CTCGTTTATA CCCAGTAGTT ATTGTTTGGG TACAGCTGAG CATGACAAAG 
CAGTCGGCTG ACAGCCAAGA AACAGACGAG TTCATGGCTC TGGCCTCAGA AGTCAAACAG 
CGAAACGTTC TGCTCTATGG AATTAAGATC GATCACTCAA AGGAGCTGGA GCAATGGCAG 
AGATCACTGG AGAGACTCGG TCAGATCACA CTCGTCCTCA TCAGAGACAG AAACATGGCT 
CTCACTGGAC AGATGCTTCA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISPD ( XM_017351046.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017206535.1 Danio rerio isoprenoid synthase domain containing (ispd), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa47290
Clone ID Related Accession (Same CDS sequence)	XM_017351046.2 , XM_017351046.1
Accession Version	XM_017351046.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	isoprenoid synthase domain-containing protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351046.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATACGG CTGCCACAAA TGCGGAGTTA ATTGAAACCA AGGAAAAAAT GCAGCAAGAG 
CGCTGTTCTT CCTCCGACAG GCCCTTGGAC CCCGGGTGTC TCCTACCACA AGACGCCGAG 
CGGAGCGCCG ACCAACCCGG GCGCGCGGTG GTGGATTTCC CGGTAGCTGC CGTTCTGCCC 
GCCGGAGGCT CCGGGGAGAG GATGGGACTG ACCACACCGA AACAATTCTG CTCCATCTTT 
AACAGACCTC TGATAAGCTA CACCATTCAA GCATTTGAGA GATTACCGTG GATCGTAATG 
GTTGTCGTTG TGGTTGCTAA GGATAATCAT GACCTGATGC TGAATATTGT CCGAAAATTT 
AATCACACAA AGGTAAAAGT AGTCCACGGG GGTACCACCA GACACCGGTC CATCTACAAC 
GGTCTTCAGG CCTTCAGCGA CTCTACAGAC TCATCGACAC CCAAACCAAA AGTGGTCATC 
ATACATGATG CAGTACGGCC ATTTGTGGAA GAAGATCTTC TGCTGAAAAT CACCCTAGCA 
GCTAAAGAAC AAGGGGCCTC AGGAGCGATT CGTCCTCTTG TGTCAACGGT CATTGCAACC 
ACGTCAGAAA GTTACCTGGA CCACTCGCTG GAGAGAGCCA AGTACCGGGC CAGTGAAATG 
CCTCAAGGAT TTCTTTATGA CATTATTTTC CAAGCCTACC AGCGGTGCAG TGAATTTGAC 
TTAGAGTTTG GCACCGAGTG TCTACATTTG GCCCTGCAGT ACTGTGGCAC GAATGCCAGG 
CTCATAGAGG GACCACCGAC TCTGTGGAAA GTAAGGAACA AAAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206535.1
CDS	315..1142
Translation	MNTAATNAELIETKEKMQQERCSSSDRPLDPGCLLPQDAERSADQPGRAVVDFPVAAVLPAGGSGERMGLTTPKQFCSIFNRPLISYTIQAFERLPWIVMVVVVVAKDNHDLMLNIVRKFNHTKVKVVHGGTTRHRSIYNGLQAFSDSTDSSTPKPKVVIIHDAVRPFVEEDLLLKITLAAKEQGASGAIRPLVSTVIATTSESYLDHSLERAKYRASEMPQGFLYDIIFQAYQRCSEFDLEFGTECLHLALQYCGTNARLIEGPPTLWKVRNKR

Target ORF information:

RefSeq Version	XM_017351046.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio isoprenoid synthase domain containing (ispd), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351046.2

ORF Insert Sequence:

ATGAATACGG CTGCCACAAA TGCGGAGTTA ATTGAAACCA AGGAAAAAAT GCAGCAAGAG 
CGCTGTTCTT CCTCCGACAG GCCCTTGGAC CCCGGGTGTC TCCTACCACA AGACGCCGAG 
CGGAGCGCCG ACCAACCCGG GCGCGCGGTG GTGGATTTCC CGGTAGCTGC CGTTCTGCCC 
GCCGGAGGCT CCGGGGAGAG GATGGGACTG ACCACACCGA AACAATTCTG CTCCATCTTT 
AACAGACCTC TGATAAGCTA CACCATTCAA GCATTTGAGA GATTACCGTG GATCGTAATG 
GTTGTCGTTG TGGTTGCTAA GGATAATCAT GACCTGATGC TGAATATTGT CCGAAAATTT 
AATCACACAA AGGTAAAAGT AGTCCACGGG GGTACCACCA GACACCGGTC CATCTACAAC 
GGTCTTCAGG CCTTCAGCGA CTCTACAGAC TCATCGACAC CCAAACCAAA AGTGGTCATC 
ATACATGATG CAGTACGGCC ATTTGTGGAA GAAGATCTTC TGCTGAAAAT CACCCTAGCA 
GCTAAAGAAC AAGGGGCCTC AGGAGCGATT CGTCCTCTTG TGTCAACGGT CATTGCAACC 
ACGTCAGAAA GTTACCTGGA CCACTCGCTG GAGAGAGCCA AGTACCGGGC CAGTGAAATG 
CCTCAAGGAT TTCTTTATGA CATTATTTTC CAAGCCTACC AGCGGTGCAG TGAATTTGAC 
TTAGAGTTTG GCACCGAGTG TCTACATTTG GCCCTGCAGT ACTGTGGCAC GAATGCCAGG 
CTCATAGAGG GACCACCGAC TCTGTGGAAA GTAAGGAACA AAAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISPD ( XM_017351046.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017206535.1 Danio rerio isoprenoid synthase domain containing (ispd), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa47290
Clone ID Related Accession (Same CDS sequence)	XM_017351046.2 , XM_017351046.1
Accession Version	XM_017351046.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	isoprenoid synthase domain-containing protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATACGG CTGCCACAAA TGCGGAGTTA ATTGAAACCA AGGAAAAAAT GCAGCAAGAG 
CGCTGTTCTT CCTCCGACAG GCCCTTGGAC CCCGGGTGTC TCCTACCACA AGACGCCGAG 
CGGAGCGCCG ACCAACCCGG GCGCGCGGTG GTGGATTTCC CGGTAGCTGC CGTTCTGCCC 
GCCGGAGGCT CCGGGGAGAG GATGGGACTG ACCACACCGA AACAATTCTG CTCCATCTTT 
AACAGACCTC TGATAAGCTA CACCATTCAA GCATTTGAGA GATTACCGTG GATCGTAATG 
GTTGTCGTTG TGGTTGCTAA GGATAATCAT GACCTGATGC TGAATATTGT CCGAAAATTT 
AATCACACAA AGGTAAAAGT AGTCCACGGG GGTACCACCA GACACCGGTC CATCTACAAC 
GGTCTTCAGG CCTTCAGCGA CTCTACAGAC TCATCGACAC CCAAACCAAA AGTGGTCATC 
ATACATGATG CAGTACGGCC ATTTGTGGAA GAAGATCTTC TGCTGAAAAT CACCCTAGCA 
GCTAAAGAAC AAGGGGCCTC AGGAGCGATT CGTCCTCTTG TGTCAACGGT CATTGCAACC 
ACGTCAGAAA GTTACCTGGA CCACTCGCTG GAGAGAGCCA AGTACCGGGC CAGTGAAATG 
CCTCAAGGAT TTCTTTATGA CATTATTTTC CAAGCCTACC AGCGGTGCAG TGAATTTGAC 
TTAGAGTTTG GCACCGAGTG TCTACATTTG GCCCTGCAGT ACTGTGGCAC GAATGCCAGG 
CTCATAGAGG GACCACCGAC TCTGTGGAAA GTAAGGAACA AAAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206535.1
CDS	333..1160
Translation	MNTAATNAELIETKEKMQQERCSSSDRPLDPGCLLPQDAERSADQPGRAVVDFPVAAVLPAGGSGERMGLTTPKQFCSIFNRPLISYTIQAFERLPWIVMVVVVVAKDNHDLMLNIVRKFNHTKVKVVHGGTTRHRSIYNGLQAFSDSTDSSTPKPKVVIIHDAVRPFVEEDLLLKITLAAKEQGASGAIRPLVSTVIATTSESYLDHSLERAKYRASEMPQGFLYDIIFQAYQRCSEFDLEFGTECLHLALQYCGTNARLIEGPPTLWKVRNKR

Target ORF information:

RefSeq Version	XM_017351046.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio isoprenoid synthase domain containing (ispd), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351046.1

ORF Insert Sequence:

ATGAATACGG CTGCCACAAA TGCGGAGTTA ATTGAAACCA AGGAAAAAAT GCAGCAAGAG 
CGCTGTTCTT CCTCCGACAG GCCCTTGGAC CCCGGGTGTC TCCTACCACA AGACGCCGAG 
CGGAGCGCCG ACCAACCCGG GCGCGCGGTG GTGGATTTCC CGGTAGCTGC CGTTCTGCCC 
GCCGGAGGCT CCGGGGAGAG GATGGGACTG ACCACACCGA AACAATTCTG CTCCATCTTT 
AACAGACCTC TGATAAGCTA CACCATTCAA GCATTTGAGA GATTACCGTG GATCGTAATG 
GTTGTCGTTG TGGTTGCTAA GGATAATCAT GACCTGATGC TGAATATTGT CCGAAAATTT 
AATCACACAA AGGTAAAAGT AGTCCACGGG GGTACCACCA GACACCGGTC CATCTACAAC 
GGTCTTCAGG CCTTCAGCGA CTCTACAGAC TCATCGACAC CCAAACCAAA AGTGGTCATC 
ATACATGATG CAGTACGGCC ATTTGTGGAA GAAGATCTTC TGCTGAAAAT CACCCTAGCA 
GCTAAAGAAC AAGGGGCCTC AGGAGCGATT CGTCCTCTTG TGTCAACGGT CATTGCAACC 
ACGTCAGAAA GTTACCTGGA CCACTCGCTG GAGAGAGCCA AGTACCGGGC CAGTGAAATG 
CCTCAAGGAT TTCTTTATGA CATTATTTTC CAAGCCTACC AGCGGTGCAG TGAATTTGAC 
TTAGAGTTTG GCACCGAGTG TCTACATTTG GCCCTGCAGT ACTGTGGCAC GAATGCCAGG 
CTCATAGAGG GACCACCGAC TCTGTGGAAA GTAAGGAACA AAAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISPD ( XM_009291808.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009290083.1 Danio rerio isoprenoid synthase domain containing (ispd), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa47291
Clone ID Related Accession (Same CDS sequence)	XM_009291808.3 , XM_009291808.2
Accession Version	XM_009291808.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	isoprenoid synthase domain-containing protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291808.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATACGG CTGCCACAAA TGCGGAGTTA ATTGAAACCA AGGAAAAAAT GCAGCAAGAG 
CGCTGTTCTT CCTCCGACAG GCCCTTGGAC CCCGGGTGTC TCCTACCACA AGACGCCGAG 
CGGAGCGCCG ACCAACCCGG GCGCGCGGTG GTGGATTTCC CGGTAGCTGC CGTTCTGCCC 
GCCGGAGGCT CCGGGGAGAG GATGGGACTG ACCACACCGA AACAATTCTG CTCCATCTTT 
AACAGACCTC TGATAAGCTA CACCATTCAA GCATTTGAGA GATTACCGTG GATCGTAATG 
GTTGTCGTTG TGGTTGCTAA GGATAATCAT GACCTGATGC TGAATATTGT CCGAAAATTT 
AATCACACAA AGGTAAAAGT AGTCCACGGG GGTACCACCA GACACCGGTC CATCTACAAC 
GGTCTTCAGG CCTTCAGCGA CTCTACAGAC TCATCGACAC CCAAACCAAA AGTGGTCATC 
ATACATGATG CAGTACGGCC ATTTGTGGAA GAAGATCTTC TGCTGAAAAT CACCCTAGCA 
GCTAAAGAAC AAGGGGCCTC AGGAGCGATT CGTCCTCTTG TGTCAACGGT CATTGCAACC 
ACGTCAGAAA GTTACCTGGA CCACTCGCTG GAGAGAGCCA AGTACCGGGC CAGTGAAATG 
CCTCAAGGAT TTCTTTATGA CATTATTTTC CAAGCCTACC AGCGGCAGAA AAAAGTAACT 
CATGAAGATT TAAACCCACA TGAAGGAGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290083.1
CDS	317..1069
Translation	MNTAATNAELIETKEKMQQERCSSSDRPLDPGCLLPQDAERSADQPGRAVVDFPVAAVLPAGGSGERMGLTTPKQFCSIFNRPLISYTIQAFERLPWIVMVVVVVAKDNHDLMLNIVRKFNHTKVKVVHGGTTRHRSIYNGLQAFSDSTDSSTPKPKVVIIHDAVRPFVEEDLLLKITLAAKEQGASGAIRPLVSTVIATTSESYLDHSLERAKYRASEMPQGFLYDIIFQAYQRQKKVTHEDLNPHEGE

Target ORF information:

RefSeq Version	XM_009291808.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio isoprenoid synthase domain containing (ispd), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291808.3

ORF Insert Sequence:

ATGAATACGG CTGCCACAAA TGCGGAGTTA ATTGAAACCA AGGAAAAAAT GCAGCAAGAG 
CGCTGTTCTT CCTCCGACAG GCCCTTGGAC CCCGGGTGTC TCCTACCACA AGACGCCGAG 
CGGAGCGCCG ACCAACCCGG GCGCGCGGTG GTGGATTTCC CGGTAGCTGC CGTTCTGCCC 
GCCGGAGGCT CCGGGGAGAG GATGGGACTG ACCACACCGA AACAATTCTG CTCCATCTTT 
AACAGACCTC TGATAAGCTA CACCATTCAA GCATTTGAGA GATTACCGTG GATCGTAATG 
GTTGTCGTTG TGGTTGCTAA GGATAATCAT GACCTGATGC TGAATATTGT CCGAAAATTT 
AATCACACAA AGGTAAAAGT AGTCCACGGG GGTACCACCA GACACCGGTC CATCTACAAC 
GGTCTTCAGG CCTTCAGCGA CTCTACAGAC TCATCGACAC CCAAACCAAA AGTGGTCATC 
ATACATGATG CAGTACGGCC ATTTGTGGAA GAAGATCTTC TGCTGAAAAT CACCCTAGCA 
GCTAAAGAAC AAGGGGCCTC AGGAGCGATT CGTCCTCTTG TGTCAACGGT CATTGCAACC 
ACGTCAGAAA GTTACCTGGA CCACTCGCTG GAGAGAGCCA AGTACCGGGC CAGTGAAATG 
CCTCAAGGAT TTCTTTATGA CATTATTTTC CAAGCCTACC AGCGGCAGAA AAAAGTAACT 
CATGAAGATT TAAACCCACA TGAAGGAGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISPD ( XM_009291808.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009290083.1 Danio rerio isoprenoid synthase domain containing (ispd), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa47291
Clone ID Related Accession (Same CDS sequence)	XM_009291808.3 , XM_009291808.2
Accession Version	XM_009291808.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	isoprenoid synthase domain-containing protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291808.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATACGG CTGCCACAAA TGCGGAGTTA ATTGAAACCA AGGAAAAAAT GCAGCAAGAG 
CGCTGTTCTT CCTCCGACAG GCCCTTGGAC CCCGGGTGTC TCCTACCACA AGACGCCGAG 
CGGAGCGCCG ACCAACCCGG GCGCGCGGTG GTGGATTTCC CGGTAGCTGC CGTTCTGCCC 
GCCGGAGGCT CCGGGGAGAG GATGGGACTG ACCACACCGA AACAATTCTG CTCCATCTTT 
AACAGACCTC TGATAAGCTA CACCATTCAA GCATTTGAGA GATTACCGTG GATCGTAATG 
GTTGTCGTTG TGGTTGCTAA GGATAATCAT GACCTGATGC TGAATATTGT CCGAAAATTT 
AATCACACAA AGGTAAAAGT AGTCCACGGG GGTACCACCA GACACCGGTC CATCTACAAC 
GGTCTTCAGG CCTTCAGCGA CTCTACAGAC TCATCGACAC CCAAACCAAA AGTGGTCATC 
ATACATGATG CAGTACGGCC ATTTGTGGAA GAAGATCTTC TGCTGAAAAT CACCCTAGCA 
GCTAAAGAAC AAGGGGCCTC AGGAGCGATT CGTCCTCTTG TGTCAACGGT CATTGCAACC 
ACGTCAGAAA GTTACCTGGA CCACTCGCTG GAGAGAGCCA AGTACCGGGC CAGTGAAATG 
CCTCAAGGAT TTCTTTATGA CATTATTTTC CAAGCCTACC AGCGGCAGAA AAAAGTAACT 
CATGAAGATT TAAACCCACA TGAAGGAGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290083.1
CDS	335..1087
Translation	MNTAATNAELIETKEKMQQERCSSSDRPLDPGCLLPQDAERSADQPGRAVVDFPVAAVLPAGGSGERMGLTTPKQFCSIFNRPLISYTIQAFERLPWIVMVVVVVAKDNHDLMLNIVRKFNHTKVKVVHGGTTRHRSIYNGLQAFSDSTDSSTPKPKVVIIHDAVRPFVEEDLLLKITLAAKEQGASGAIRPLVSTVIATTSESYLDHSLERAKYRASEMPQGFLYDIIFQAYQRQKKVTHEDLNPHEGE

Target ORF information:

RefSeq Version	XM_009291808.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio isoprenoid synthase domain containing (ispd), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291808.2

ORF Insert Sequence:

ATGAATACGG CTGCCACAAA TGCGGAGTTA ATTGAAACCA AGGAAAAAAT GCAGCAAGAG 
CGCTGTTCTT CCTCCGACAG GCCCTTGGAC CCCGGGTGTC TCCTACCACA AGACGCCGAG 
CGGAGCGCCG ACCAACCCGG GCGCGCGGTG GTGGATTTCC CGGTAGCTGC CGTTCTGCCC 
GCCGGAGGCT CCGGGGAGAG GATGGGACTG ACCACACCGA AACAATTCTG CTCCATCTTT 
AACAGACCTC TGATAAGCTA CACCATTCAA GCATTTGAGA GATTACCGTG GATCGTAATG 
GTTGTCGTTG TGGTTGCTAA GGATAATCAT GACCTGATGC TGAATATTGT CCGAAAATTT 
AATCACACAA AGGTAAAAGT AGTCCACGGG GGTACCACCA GACACCGGTC CATCTACAAC 
GGTCTTCAGG CCTTCAGCGA CTCTACAGAC TCATCGACAC CCAAACCAAA AGTGGTCATC 
ATACATGATG CAGTACGGCC ATTTGTGGAA GAAGATCTTC TGCTGAAAAT CACCCTAGCA 
GCTAAAGAAC AAGGGGCCTC AGGAGCGATT CGTCCTCTTG TGTCAACGGT CATTGCAACC 
ACGTCAGAAA GTTACCTGGA CCACTCGCTG GAGAGAGCCA AGTACCGGGC CAGTGAAATG 
CCTCAAGGAT TTCTTTATGA CATTATTTTC CAAGCCTACC AGCGGCAGAA AAAAGTAACT 
CATGAAGATT TAAACCCACA TGAAGGAGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A limb-girdle muscular dystrophy 2I model of muscular dystrophy identifies corrective drug compounds for dystroglycanopathies.
JCI insight3(18)(2018 09)
Serafini PR,Feyder MJ,Hightower RM,Garcia-Perez D,Vieira NM,Lek A,Gibbs DE,Moukha-Chafiq O,Augelli-Szafran CE,Kawahara G,Widrick JJ,Kunkel LM,Alexander MS

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Mutations in ISPD cause Walker-Warburg syndrome and defective glycosylation of α-dystroglycan.
Nature genetics44(5)581-5(2012 May)
Roscioli T,Kamsteeg EJ,Buysse K,Maystadt I,van Reeuwijk J,van den Elzen C,van Beusekom E,Riemersma M,Pfundt R,Vissers LE,Schraders M,Altunoglu U,Buckley MF,Brunner HG,Grisart B,Zhou H,Veltman JA,Gilissen C,Mancini GM,Delrée P,Willemsen MA,Ramadža DP,Chitayat D,Bennett C,Sheridan E,Peeters EA,Tan-Sindhunata GM,de Die-Smulders CE,Devriendt K,Kayserili H,El-Hashash OA,Stemple DL,Lefeber DJ,Lin YY,van Bokhoven H

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ISPD cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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