rbl1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rbl1
Entrez Gene ID	795522
Full Name	retinoblastoma-like 1 (p107)
Synonyms	fj58e06,wu:fj58e06
General protein information	Preferred Names retinoblastoma-like 1 (p107) Names retinoblastoma-like protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021476946.1	XP_021332621.1	retinoblastoma-like protein 1 isoform X1
NM_001130610.1	NP_001124082.1	retinoblastoma-like protein 1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os11g0533500	NP_001068020.1
Bos taurus (cattle)	RBL1	NP_001179531.1
Mus musculus (house mouse)	Rbl1	NP_035379.2
Danio rerio (zebrafish)	rbl1	NP_001124082.1
Xenopus tropicalis (tropical clawed frog)	rbl1	NP_001135587.1
Homo sapiens (human)	RBL1	NP_002886.2
Macaca mulatta (Rhesus monkey)	RBL1	XP_002798195.1
Rattus norvegicus (Norway rat)	Rbl1	NP_001177995.1
Caenorhabditis elegans (roundworm)	lin-35	NP_491686.1
Arabidopsis thaliana (thale cress)	RBR1	NP_566417.3
Pan troglodytes (chimpanzee)	RBL1	XP_001139919.1
Canis lupus familiaris (dog)	RBL1	XP_534412.2
Gallus gallus (chicken)	RBL1	XP_417312.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

High-throughput genome editing and phenotyping facilitated by high resolution melting curve analysis.
Thomas HR, Percival SM, Yoder BK, Parant JM
PloS one9(12)e114632(2014)

Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish.
Hu SN, Yu H, Zhang YB, Wu ZL, Yan YC, Li YX, Li YY, Li YP
FEBS letters586(3)222-8(2012 Feb)

Coordinate control of cell cycle regulatory genes in zebrafish development tested by cyclin D1 knockdown with morpholino phosphorodiamidates and hydroxyprolyl-phosphono peptide nucleic acids.
Duffy KT, McAleer MF, Davidson WR, Kari L, Kari C, Liu CG, Farber SA, Cheng KC, Mest JR, Wickstrom E, Dicker AP, Rodeck U
Nucleic acids research33(15)4914-21(2005)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rbl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rbl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36447	NM_001130610.1 Latest version!	Danio rerio retinoblastoma-like 1 (p107) (rbl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa142993	XM_021476946.1 Latest version!	Danio rerio retinoblastoma-like 1 (p107) (rbl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rbl1 ( NM_001130610.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001124082.1 Danio rerio retinoblastoma-like 1 (p107) (rbl1), mRNA.

Price & Availability↑

CloneID	ODa36447
Clone ID Related Accession (Same CDS sequence)	NM_001130610.1
Accession Version	NM_001130610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	retinoblastoma-like protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC162986.1. On Jul 30, 2008 this sequence version replaced XM_001333003.2. ##Evidence-Data-START## Transcript exon combination :: BC162986.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCGTGGGG ATGAAACGGA TACAGAGTCC GTCAGATCAG CCGATGGCTC GATCCGGAGT 
AATTTCGAGG CGCTTTGTCA GGAGCTCAAT ATGGACGAAG AGACGGCAGC TGAAGCGCTT 
CAGAACTTCA GATCCATCTG GAACACTTAC ACGCTGGAGG GTGATGTCAT GCACTGGCTG 
GCCTGCTCTC TGTACGCTGC CTGTAGGAAG AGCTCCATCC CTACAGTGGG ACGGGGTGTG 
ATGGAGGGCA ACTGTGTTTC CCTTACAAGG ATCTTGCGCT CTGCAAAACT CAGTTTGATC 
CAGTTCTTCA GCAAGATGAA GAAATGGTCA GACATGGCGA ATCTCTGTCA GGACTTTCGC 
AGCCGCATAG GTCGACTAGA GCGTAATTTT GAAGTGTCGA CAGTGATTTT TCGCAAATTT 
GAGCCGATAT TTCTGGACAT GTTTCAGAAT CCTCAGAGTG AACCTCCTCG CATGCCTAGA 
AGTCGCAAAC ACCGACGTCT TCCATGCCAC ATCAGTGATG TCTTCAAGTT CTGTTGGACC 
CTCTTTGTTT ATACAAAAGG TAATTTCCAC ATGATCGGAG ATGATTTGGT CAACTCCTAT 
CACCTCCTCC TCTGCTGTCT GGACCTGGTG TTTTGCAACG CTCTCATGTG CTCCAACAGG 
AAAGATCTAA TCAATCAGAA TTTCAGAGGC CTGCCGAAAG ACACAGAAAA CCTGAAAGAG 
ATGCCCTGTG TCATTGACAA GTTGTGTGAA CTGCATGATG GGTTGGTGGT GGAGGCCAAA 
GGCATAAAGG AGCACTACTT CAAGCCCTAC ATAAAGACCC TGTTTGAGAA AAGAATGTTA 
AAAGGTGATG TTGAAACTCT GACTGAATTA CTGGACACTC CAAATTTCCA AGATAACAAT 
AAAGCCATAA ATCGTGATTA TGAGGAGTTT GTTTTGACTG TGGGGGATTT TGATGAGCGA 
GTGTTTTTGG GGGCTGATGC AGACGAGGAG ATCGGCACAC CGCGCAAATC CCCCGCTGAA 
CCTCCTTCAG GTCATCTCTC TGCCCGCATG CAGGTGGAGA ACAACCTTCA GCAGCACTTT 
GAGAAGACAC GTTCCCTTGC TCCCTCTACT CCTCTAACGG GTCGACGGTA TTTAAAGGAG 
AAGGAAGTGC TGGTCACGCC TGTTTCTTCA GCCACCCAAA GTGTGAGTCG ACTGCAAAGC 
ATGGTGTCTG GTCTCCGAAA CGCACCGAGC GACGCTCTGC TCCAGATATT TAATTCCTGT 
TCTCGAAACC CCACTGAATC CATTCTGAAT CGTGTGAAAA CCTTGGGGGA AAAGTTCAAA 
CAGGCCTACA CTAATCCCAC AGATGACCTG CCGGGAGCAC ATATGGACTT TGCAGAGAAA 
CGTCTGAAGC TGGCCGAGAT CTTGTACTTT AAGATCCTGG AGAATGTAAT GACTCAAGAG 
ATGAAGAGAT TACAAGGCAA AGACTGGGCA GTCCTGTTGG AGCAGGAGGT GCTCCATTGC 
TCTCTTTTGG CCTGCTGTCT GGAGGTGGTG CTGTTCTCAT ACAGCTCACA AAGAACTTTC 
CCCTGGATAC TGGAAATCTT TCAGATCCCA CCTTTCTACT TTTACAAGGT GATTGAGGTG 
TTCATCCGCT CAGAGGAGGG TTTATCCAGA GACATGGTGA AGCATTTGAA CAGTATAGAG 
GAACAGGTGC TGGAAAGCAG AGCGTGGACA AGAGATTCAG CCCTGTGGAA TGCTCTTAAC 
AAGGATAACA ATAAAGTTCC TACTGTAGAA GAGGTGAATT TTCCTAGCAA TTTTGATACA 
GGCAACAATG CAGGTGGTCC CACTCACCTC CCATTGGTGG CGCTGTCCCC CATCATTCAC 
CCTCGCCTCA GGGAGGTCCG CACGGGCCTG GGGTCCAGCG CACGCAAAGA CGTCCCTCAA 
TCCCCCATCT CTCTCCATGA TCGCTACAGC TCTCCAGCTG CTGGCAGCGC AAAGAGACGC 
TTGTTTGGTG ACGACGCTCC ACCACAATCC CCAGTAAAAA GGATCTCTGT CACTCCCATC 
AAAATCATTC CCAGTGGCAT GGAAAACCAG ACCACCACAA CCACTGTCCT CTCCATGGCC 
ACCGGAAATG GCCAGCAGCT CACCATTCCA CTGCCAGTGG TGAAGAATGA GGCAGGCGGC 
ATCACAGTCA TCCAGCTTCA GGCCAGCGAT ATGAATCCTC TCACTGCTCA AGTTTTATTG 
ACCGCTTCCC CGAGCCGGCC TGCTCTAGCC GCTCCAGCTA TCAGCTCTGA CTCACAGCCA 
CCACCTGCCA ATAAACCTCG CCGCACGGGC TCACTCGCAC TCTTCTTCAG GAAGGTGTAT 
CATTTAGCCA GTGTGCGTCT GCGAGACCTG TGTCTGAAGC TGGATATCTC CTCAGAGCTA 
AGAGGGAAGA TCTGGACGTG TTTTGAGCAC TCTCTCCTGC ACTGCACTGA CCTGATGAAG 
GACAGACACC TGGACCAGCT GCTGCTCTGT GCTGTTTACA TCATATCAAG GATCACGAAA 
GAAGAGCACA CTTTTCAGGA CATTATGAAA TGCTACCGAA CACAACCACA GGCCAACAGT 
CATGTGTACC GCAGTGTTTT AATAAAGAGG CGTCCCAAAG TGCAGCAGGC TGACGAGAAC 
ATGGAAGTCG ATCCTCCAGC AGACCAGTCC AATGAGAGGA CAGATCAGGC AGCCAATGAG 
AGGACCGATC AGGCTGACAG AACACAGACT GTCTTCGAAT CAGAGGAAAG AGGGGATCTC 
ATCCAGTTCT ACAACAACAT TTATGTGTCA AAGATGAGAT CATTTGCTTT TAAATATGCA 
CTTTCCACGC TTGATAACAG GATGGAAGCT CCTCCGTTAT CTCCGTTCCC CTCCGTTCGG 
TCTCACACTC TCTCTCCTCG CCGTGTTTCT CAGAAACACT CCATCTTTGT GTCTCCTCAT 
AAAAACAGCT CCAGTCTCAC GCCCAGCACT GCATACACGT ACAGGTTCAC TGGAAGCCCT 
TCAAAGGAGC TGAGTGACAT AAACCAGATG ATCCGTCAGG GTGGCGTGAG CAAGAAGCGG 
GCATTCACCA TGGAGGGCGA CGAGACCGAA TCTCCGTCTA AATGTCTCCG GCAAGAGAAC 
GACGACGTGC TGCTCAAACG CCTCCAGGAC GTGGTCAGCG AGAGAGCCAG TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124082.1
CDS	96..3272
Translation	MRGDETDTESVRSADGSIRSNFEALCQELNMDEETAAEALQNFRSIWNTYTLEGDVMHWLACSLYAACRKSSIPTVGRGVMEGNCVSLTRILRSAKLSLIQFFSKMKKWSDMANLCQDFRSRIGRLERNFEVSTVIFRKFEPIFLDMFQNPQSEPPRMPRSRKHRRLPCHISDVFKFCWTLFVYTKGNFHMIGDDLVNSYHLLLCCLDLVFCNALMCSNRKDLINQNFRGLPKDTENLKEMPCVIDKLCELHDGLVVEAKGIKEHYFKPYIKTLFEKRMLKGDVETLTELLDTPNFQDNNKAINRDYEEFVLTVGDFDERVFLGADADEEIGTPRKSPAEPPSGHLSARMQVENNLQQHFEKTRSLAPSTPLTGRRYLKEKEVLVTPVSSATQSVSRLQSMVSGLRNAPSDALLQIFNSCSRNPTESILNRVKTLGEKFKQAYTNPTDDLPGAHMDFAEKRLKLAEILYFKILENVMTQEMKRLQGKDWAVLLEQEVLHCSLLACCLEVVLFSYSSQRTFPWILEIFQIPPFYFYKVIEVFIRSEEGLSRDMVKHLNSIEEQVLESRAWTRDSALWNALNKDNNKVPTVEEVNFPSNFDTGNNAGGPTHLPLVALSPIIHPRLREVRTGLGSSARKDVPQSPISLHDRYSSPAAGSAKRRLFGDDAPPQSPVKRISVTPIKIIPSGMENQTTTTTVLSMATGNGQQLTIPLPVVKNEAGGITVIQLQASDMNPLTAQVLLTASPSRPALAAPAISSDSQPPPANKPRRTGSLALFFRKVYHLASVRLRDLCLKLDISSELRGKIWTCFEHSLLHCTDLMKDRHLDQLLLCAVYIISRITKEEHTFQDIMKCYRTQPQANSHVYRSVLIKRRPKVQQADENMEVDPPADQSNERTDQAANERTDQADRTQTVFESEERGDLIQFYNNIYVSKMRSFAFKYALSTLDNRMEAPPLSPFPSVRSHTLSPRRVSQKHSIFVSPHKNSSSLTPSTAYTYRFTGSPSKELSDINQMIRQGGVSKKRAFTMEGDETESPSKCLRQENDDVLLKRLQDVVSERASL

Target ORF information:

RefSeq Version	NM_001130610.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio retinoblastoma-like 1 (p107) (rbl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130610.1

ORF Insert Sequence:

ATGCGTGGGG ATGAAACGGA TACAGAGTCC GTCAGATCAG CCGATGGCTC GATCCGGAGT 
AATTTCGAGG CGCTTTGTCA GGAGCTCAAT ATGGACGAAG AGACGGCAGC TGAAGCGCTT 
CAGAACTTCA GATCCATCTG GAACACTTAC ACGCTGGAGG GTGATGTCAT GCACTGGCTG 
GCCTGCTCTC TGTACGCTGC CTGTAGGAAG AGCTCCATCC CTACAGTGGG ACGGGGTGTG 
ATGGAGGGCA ACTGTGTTTC CCTTACAAGG ATCTTGCGCT CTGCAAAACT CAGTTTGATC 
CAGTTCTTCA GCAAGATGAA GAAATGGTCA GACATGGCGA ATCTCTGTCA GGACTTTCGC 
AGCCGCATAG GTCGACTAGA GCGTAATTTT GAAGTGTCGA CAGTGATTTT TCGCAAATTT 
GAGCCGATAT TTCTGGACAT GTTTCAGAAT CCTCAGAGTG AACCTCCTCG CATGCCTAGA 
AGTCGCAAAC ACCGACGTCT TCCATGCCAC ATCAGTGATG TCTTCAAGTT CTGTTGGACC 
CTCTTTGTTT ATACAAAAGG TAATTTCCAC ATGATCGGAG ATGATTTGGT CAACTCCTAT 
CACCTCCTCC TCTGCTGTCT GGACCTGGTG TTTTGCAACG CTCTCATGTG CTCCAACAGG 
AAAGATCTAA TCAATCAGAA TTTCAGAGGC CTGCCGAAAG ACACAGAAAA CCTGAAAGAG 
ATGCCCTGTG TCATTGACAA GTTGTGTGAA CTGCATGATG GGTTGGTGGT GGAGGCCAAA 
GGCATAAAGG AGCACTACTT CAAGCCCTAC ATAAAGACCC TGTTTGAGAA AAGAATGTTA 
AAAGGTGATG TTGAAACTCT GACTGAATTA CTGGACACTC CAAATTTCCA AGATAACAAT 
AAAGCCATAA ATCGTGATTA TGAGGAGTTT GTTTTGACTG TGGGGGATTT TGATGAGCGA 
GTGTTTTTGG GGGCTGATGC AGACGAGGAG ATCGGCACAC CGCGCAAATC CCCCGCTGAA 
CCTCCTTCAG GTCATCTCTC TGCCCGCATG CAGGTGGAGA ACAACCTTCA GCAGCACTTT 
GAGAAGACAC GTTCCCTTGC TCCCTCTACT CCTCTAACGG GTCGACGGTA TTTAAAGGAG 
AAGGAAGTGC TGGTCACGCC TGTTTCTTCA GCCACCCAAA GTGTGAGTCG ACTGCAAAGC 
ATGGTGTCTG GTCTCCGAAA CGCACCGAGC GACGCTCTGC TCCAGATATT TAATTCCTGT 
TCTCGAAACC CCACTGAATC CATTCTGAAT CGTGTGAAAA CCTTGGGGGA AAAGTTCAAA 
CAGGCCTACA CTAATCCCAC AGATGACCTG CCGGGAGCAC ATATGGACTT TGCAGAGAAA 
CGTCTGAAGC TGGCCGAGAT CTTGTACTTT AAGATCCTGG AGAATGTAAT GACTCAAGAG 
ATGAAGAGAT TACAAGGCAA AGACTGGGCA GTCCTGTTGG AGCAGGAGGT GCTCCATTGC 
TCTCTTTTGG CCTGCTGTCT GGAGGTGGTG CTGTTCTCAT ACAGCTCACA AAGAACTTTC 
CCCTGGATAC TGGAAATCTT TCAGATCCCA CCTTTCTACT TTTACAAGGT GATTGAGGTG 
TTCATCCGCT CAGAGGAGGG TTTATCCAGA GACATGGTGA AGCATTTGAA CAGTATAGAG 
GAACAGGTGC TGGAAAGCAG AGCGTGGACA AGAGATTCAG CCCTGTGGAA TGCTCTTAAC 
AAGGATAACA ATAAAGTTCC TACTGTAGAA GAGGTGAATT TTCCTAGCAA TTTTGATACA 
GGCAACAATG CAGGTGGTCC CACTCACCTC CCATTGGTGG CGCTGTCCCC CATCATTCAC 
CCTCGCCTCA GGGAGGTCCG CACGGGCCTG GGGTCCAGCG CACGCAAAGA CGTCCCTCAA 
TCCCCCATCT CTCTCCATGA TCGCTACAGC TCTCCAGCTG CTGGCAGCGC AAAGAGACGC 
TTGTTTGGTG ACGACGCTCC ACCACAATCC CCAGTAAAAA GGATCTCTGT CACTCCCATC 
AAAATCATTC CCAGTGGCAT GGAAAACCAG ACCACCACAA CCACTGTCCT CTCCATGGCC 
ACCGGAAATG GCCAGCAGCT CACCATTCCA CTGCCAGTGG TGAAGAATGA GGCAGGCGGC 
ATCACAGTCA TCCAGCTTCA GGCCAGCGAT ATGAATCCTC TCACTGCTCA AGTTTTATTG 
ACCGCTTCCC CGAGCCGGCC TGCTCTAGCC GCTCCAGCTA TCAGCTCTGA CTCACAGCCA 
CCACCTGCCA ATAAACCTCG CCGCACGGGC TCACTCGCAC TCTTCTTCAG GAAGGTGTAT 
CATTTAGCCA GTGTGCGTCT GCGAGACCTG TGTCTGAAGC TGGATATCTC CTCAGAGCTA 
AGAGGGAAGA TCTGGACGTG TTTTGAGCAC TCTCTCCTGC ACTGCACTGA CCTGATGAAG 
GACAGACACC TGGACCAGCT GCTGCTCTGT GCTGTTTACA TCATATCAAG GATCACGAAA 
GAAGAGCACA CTTTTCAGGA CATTATGAAA TGCTACCGAA CACAACCACA GGCCAACAGT 
CATGTGTACC GCAGTGTTTT AATAAAGAGG CGTCCCAAAG TGCAGCAGGC TGACGAGAAC 
ATGGAAGTCG ATCCTCCAGC AGACCAGTCC AATGAGAGGA CAGATCAGGC AGCCAATGAG 
AGGACCGATC AGGCTGACAG AACACAGACT GTCTTCGAAT CAGAGGAAAG AGGGGATCTC 
ATCCAGTTCT ACAACAACAT TTATGTGTCA AAGATGAGAT CATTTGCTTT TAAATATGCA 
CTTTCCACGC TTGATAACAG GATGGAAGCT CCTCCGTTAT CTCCGTTCCC CTCCGTTCGG 
TCTCACACTC TCTCTCCTCG CCGTGTTTCT CAGAAACACT CCATCTTTGT GTCTCCTCAT 
AAAAACAGCT CCAGTCTCAC GCCCAGCACT GCATACACGT ACAGGTTCAC TGGAAGCCCT 
TCAAAGGAGC TGAGTGACAT AAACCAGATG ATCCGTCAGG GTGGCGTGAG CAAGAAGCGG 
GCATTCACCA TGGAGGGCGA CGAGACCGAA TCTCCGTCTA AATGTCTCCG GCAAGAGAAC 
GACGACGTGC TGCTCAAACG CCTCCAGGAC GTGGTCAGCG AGAGAGCCAG TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rbl1 ( XM_021476946.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332621.1 Danio rerio retinoblastoma-like 1 (p107) (rbl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa142993
Clone ID Related Accession (Same CDS sequence)	XM_021476946.1
Accession Version	XM_021476946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	retinoblastoma-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGGG ATGAAACGGA TACAGAGTCC GTCAGATCAG CCGATGGCTC GATCCGGAGT 
AATTTCGAGG CGCTTTGTCA GGAGCTCAAT ATGGACGAAG AGACGGCAGC TGAAGCGCTT 
CAGAACTTCA GATCCATCTG GAACACTTAC ACGCTGGAGG GTGATGTCAT GCACTGGCTG 
GCCTGCTCTC TGTACGCTGC CTGTAGGAAG AGCTCCATCC CTACAGTGGG ACGGGGTGTG 
ATGGAGGGCA ACTGTGTTTC CCTTACAAGG ATCTTGCGCT CTGCAAAACT CAGTTTGATC 
CAGTTCTTCA GCAAGATGAA GAAATGGTCA GACATGGCGA ATCTCTGTCA GGACTTTCGC 
AGCCGCATAG GTCGACTAGA GCGCAATTTT GAAGTGTCGA CAGTGATTTT TCGCAAATTT 
GAGCCGATAT TTCTGGACAT GTTTCAGAAT CCTCAGAGTG AACCTCCTCG CATGCCTAGA 
AGTCGCAAAC ACCGACGTCT TCCATGCCAC ATCAGTGATG TCTTCAAGTT CTGTTGGACC 
CTCTTTGTTT ATACAAAAGG TAATTTCCAC ATGATCGGAG ATGATTTGGT CAACTCCTAT 
CACCTCCTCC TCTGCTGTCT GGACCTGGTG TTTTGCAACG CTCTCATGTG CTCCAACAGG 
AAAGATCTCA TCAATCAGAA TTTCAGAGGC CTGCCGAAAG ACACAGAAAA CCTGAAAGAG 
ATGCCCTGTG TCATTGACAA GTTGTGTGAA CTGCATGATG GGTTGGTGGT GGAGGCCAAA 
GGCATAAAGG AGCACTACTT CAAGCCCTAC ATAAAGACCC TGTTTGAGAA AAGAATGTTA 
AAAGGTGATG TTGAAACTCT GACTGAATTA CTGGACACTC CAAATTTCCA AGATAACAAT 
AAAGCCATAA ATCGTGATTA TGAGGAGTTT GTTTTGACTG TGGGGGATTT TGATGAGCGA 
GTGTTTTTGG GGGCTGATGC AGACGAGGAG ATCGGCACAC CGCGCAAATC CACCGCTGAA 
CCTCCTTCAG GTCATCTCTC TGCCCGCATG CAGGTGGAGA ACAACCTTCA GCAGCACTTT 
GAGAAGACGC GTTCCCTTGC TCCCTCTACT CCTCTAACGG GTCGACGGTA TTTAAAGGAG 
AAGGAAGTGC TGGTCACGCC TGTTTCTTCA GCCACCCAAA GTGTGAGTCG ACTGCAAAGC 
ATGGTGTCTG GTCTCCGAAA CGCACCGAGC GACGCTCTGC TTCAGATATT TATTTCCTGT 
TCTCGAAACC CCACTGAATC CATTCTGAAT CGGGTGAAAA CCTTGGGGGA AAAGTTCAAA 
CAGGCCTACA CTAATCCCAC AGATGACCTG CCGGGAGCAC ATATGGACTT TGCAGAGAAA 
CGTCTGAAGC TGGCCGAGAT CTTGTACTTT AAGATCCTGG AGAATGTAAT GACTCAAGAG 
ATGAAGAGAT TACAAGGCAA AGACTGGGCA GTCCTGTTGG AGCAGGAGGT GCTCCATTGC 
TCTCTTTTGG CCTGCTGTCT GGAGGTGGTG CTGTTCTCAT ACAGCTCGCA AAGAACTTTC 
CCCTGGATAC TGGAAATCTT TCAGATCCCA CCTTTCTACT TTTACAAGGT GATTGAGGTG 
TTCATCCGCT CAGAGGAGGG TTTATCCAGA GACATGGTGA AGCATTTGAA CAGTATAGAG 
GAACAGGTGC TGGAAAGCAG AGCGTGGACA AGAGATTCAG CCCTGTGGAA TGCTCTTAAC 
AAGGATAACA ATAAAGTTCC TACTGTAGAA GAGGTGAATT TTCCTAGCAA TTTTGATACA 
GGCAACAATG CAGGTGGTCC CACTCACCTC CCATTGGTGG CGCTGTCCCC CATCATTCAC 
CCTCGCCTCA GGGAGGTCCG CACTGGCCTG GGCTCCAGCG CACGCAAAGA CGTCCCTCAA 
TCCCCCATCT CTCTCCATGA TCGCTACAGC TCTCCAGCTG CTGGCAGCGC AAAGAGACGC 
TTGTTTGGTG ACGATGCTCC ACCACAATCC CCAGTAAAAA GGATCTCTGT CACTCCCATC 
AAAATCATTC CCAGTGGCTT GGAAAACCAG ACCACCACAA CCACTGTCCT CTCCATGGCC 
ACCGGAAATG GCCAGCAGCT CACCATTCCA CTGCCAGTGG TGAAGAATGA GGCAGGCGGC 
ATCACAGTCA TCCAGCTTCA GGCCAGCGAT ATGAATCCTC TCACTGCTCA AGTTTTATTG 
ACCGCTTCCC CGAGCCGGCC TGCTCTAGCC GCTCCAGCTA TCAGCTCTGA CTCACAGCCA 
CCACCTGCCA ATAAACCTCG CCGCACGGGC TCACTCGCAC TCTTCTTCAG GAAGGTGTAT 
CATTTAGCCA GTGTGCGTCT GCGAGACCTG TGTCTGAAGC TGGATATCTC CTCAGAGCTA 
AGAGGGAAGA TCTGGACGTG TTTTGAGCAC TCTCTCCTGC ACTGCACTGA CCTGATGAAG 
GACAGACACC TGGACCAGCT GCTGCTTTGT GCTGTTTACA TCATATCAAG GATCACGAAA 
GAAGAGCACA CTTTTCAGGA CATTATGAAA TGCTACCGAA CACAACCACA GGCCAACAGT 
CATGTGTACC GCAGTGTTTT AATAAAGAGG CGTCCCAAAG TGCAGCAGGC TGACGAGAAC 
ATGGAAGTCG ATCCTCCAGC AGACCAGTCC AATGAGAGGA GCGATCAGGC TGACAGAACA 
CAGACTGTCT TCGAATCAGA GGAAAGAGGG GATCTCATCC AGTTCTACAA CAACATTTAT 
GTGTCAAAGA TGAGATCATT CGCTTTCAAA TATGCACTTT CCACGCTTGA TAACAGGATG 
GAAGCTCCTC CGTTATCTCC GTTCCCCTCC GTTCGGTCTC ACACTCTCTC TCCTCGCCGT 
GTGTCTCAGA AACACTCCAT CTTTGTGTCT CCTCATAAAA ACAGCTCCAG TCTCACGCCC 
AGCACTGCAT ACACATACAG GTTCACTGGA AGCCCTTCAA AGGAGCTGAG TGACATAAAC 
CAGATGATCC GTCAGGGTGG CGTGAGCAAG AAGCGGGCGT TCACCATGGA GGGCGACGAG 
ACCGAATCTC CGTCTAAATG TCTCCGGCAA GAGAACGACG ACGTGCTGCT CAAACGCCTC 
CAGGACGTGG TCAGCGAGAG AGCCAGTCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332621.1
CDS	136..3288
Translation	MRGDETDTESVRSADGSIRSNFEALCQELNMDEETAAEALQNFRSIWNTYTLEGDVMHWLACSLYAACRKSSIPTVGRGVMEGNCVSLTRILRSAKLSLIQFFSKMKKWSDMANLCQDFRSRIGRLERNFEVSTVIFRKFEPIFLDMFQNPQSEPPRMPRSRKHRRLPCHISDVFKFCWTLFVYTKGNFHMIGDDLVNSYHLLLCCLDLVFCNALMCSNRKDLINQNFRGLPKDTENLKEMPCVIDKLCELHDGLVVEAKGIKEHYFKPYIKTLFEKRMLKGDVETLTELLDTPNFQDNNKAINRDYEEFVLTVGDFDERVFLGADADEEIGTPRKSTAEPPSGHLSARMQVENNLQQHFEKTRSLAPSTPLTGRRYLKEKEVLVTPVSSATQSVSRLQSMVSGLRNAPSDALLQIFISCSRNPTESILNRVKTLGEKFKQAYTNPTDDLPGAHMDFAEKRLKLAEILYFKILENVMTQEMKRLQGKDWAVLLEQEVLHCSLLACCLEVVLFSYSSQRTFPWILEIFQIPPFYFYKVIEVFIRSEEGLSRDMVKHLNSIEEQVLESRAWTRDSALWNALNKDNNKVPTVEEVNFPSNFDTGNNAGGPTHLPLVALSPIIHPRLREVRTGLGSSARKDVPQSPISLHDRYSSPAAGSAKRRLFGDDAPPQSPVKRISVTPIKIIPSGLENQTTTTTVLSMATGNGQQLTIPLPVVKNEAGGITVIQLQASDMNPLTAQVLLTASPSRPALAAPAISSDSQPPPANKPRRTGSLALFFRKVYHLASVRLRDLCLKLDISSELRGKIWTCFEHSLLHCTDLMKDRHLDQLLLCAVYIISRITKEEHTFQDIMKCYRTQPQANSHVYRSVLIKRRPKVQQADENMEVDPPADQSNERSDQADRTQTVFESEERGDLIQFYNNIYVSKMRSFAFKYALSTLDNRMEAPPLSPFPSVRSHTLSPRRVSQKHSIFVSPHKNSSSLTPSTAYTYRFTGSPSKELSDINQMIRQGGVSKKRAFTMEGDETESPSKCLRQENDDVLLKRLQDVVSERASL

Target ORF information:

RefSeq Version	XM_021476946.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio retinoblastoma-like 1 (p107) (rbl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476946.1

ORF Insert Sequence:

ATGCGTGGGG ATGAAACGGA TACAGAGTCC GTCAGATCAG CCGATGGCTC GATCCGGAGT 
AATTTCGAGG CGCTTTGTCA GGAGCTCAAT ATGGACGAAG AGACGGCAGC TGAAGCGCTT 
CAGAACTTCA GATCCATCTG GAACACTTAC ACGCTGGAGG GTGATGTCAT GCACTGGCTG 
GCCTGCTCTC TGTACGCTGC CTGTAGGAAG AGCTCCATCC CTACAGTGGG ACGGGGTGTG 
ATGGAGGGCA ACTGTGTTTC CCTTACAAGG ATCTTGCGCT CTGCAAAACT CAGTTTGATC 
CAGTTCTTCA GCAAGATGAA GAAATGGTCA GACATGGCGA ATCTCTGTCA GGACTTTCGC 
AGCCGCATAG GTCGACTAGA GCGCAATTTT GAAGTGTCGA CAGTGATTTT TCGCAAATTT 
GAGCCGATAT TTCTGGACAT GTTTCAGAAT CCTCAGAGTG AACCTCCTCG CATGCCTAGA 
AGTCGCAAAC ACCGACGTCT TCCATGCCAC ATCAGTGATG TCTTCAAGTT CTGTTGGACC 
CTCTTTGTTT ATACAAAAGG TAATTTCCAC ATGATCGGAG ATGATTTGGT CAACTCCTAT 
CACCTCCTCC TCTGCTGTCT GGACCTGGTG TTTTGCAACG CTCTCATGTG CTCCAACAGG 
AAAGATCTCA TCAATCAGAA TTTCAGAGGC CTGCCGAAAG ACACAGAAAA CCTGAAAGAG 
ATGCCCTGTG TCATTGACAA GTTGTGTGAA CTGCATGATG GGTTGGTGGT GGAGGCCAAA 
GGCATAAAGG AGCACTACTT CAAGCCCTAC ATAAAGACCC TGTTTGAGAA AAGAATGTTA 
AAAGGTGATG TTGAAACTCT GACTGAATTA CTGGACACTC CAAATTTCCA AGATAACAAT 
AAAGCCATAA ATCGTGATTA TGAGGAGTTT GTTTTGACTG TGGGGGATTT TGATGAGCGA 
GTGTTTTTGG GGGCTGATGC AGACGAGGAG ATCGGCACAC CGCGCAAATC CACCGCTGAA 
CCTCCTTCAG GTCATCTCTC TGCCCGCATG CAGGTGGAGA ACAACCTTCA GCAGCACTTT 
GAGAAGACGC GTTCCCTTGC TCCCTCTACT CCTCTAACGG GTCGACGGTA TTTAAAGGAG 
AAGGAAGTGC TGGTCACGCC TGTTTCTTCA GCCACCCAAA GTGTGAGTCG ACTGCAAAGC 
ATGGTGTCTG GTCTCCGAAA CGCACCGAGC GACGCTCTGC TTCAGATATT TATTTCCTGT 
TCTCGAAACC CCACTGAATC CATTCTGAAT CGGGTGAAAA CCTTGGGGGA AAAGTTCAAA 
CAGGCCTACA CTAATCCCAC AGATGACCTG CCGGGAGCAC ATATGGACTT TGCAGAGAAA 
CGTCTGAAGC TGGCCGAGAT CTTGTACTTT AAGATCCTGG AGAATGTAAT GACTCAAGAG 
ATGAAGAGAT TACAAGGCAA AGACTGGGCA GTCCTGTTGG AGCAGGAGGT GCTCCATTGC 
TCTCTTTTGG CCTGCTGTCT GGAGGTGGTG CTGTTCTCAT ACAGCTCGCA AAGAACTTTC 
CCCTGGATAC TGGAAATCTT TCAGATCCCA CCTTTCTACT TTTACAAGGT GATTGAGGTG 
TTCATCCGCT CAGAGGAGGG TTTATCCAGA GACATGGTGA AGCATTTGAA CAGTATAGAG 
GAACAGGTGC TGGAAAGCAG AGCGTGGACA AGAGATTCAG CCCTGTGGAA TGCTCTTAAC 
AAGGATAACA ATAAAGTTCC TACTGTAGAA GAGGTGAATT TTCCTAGCAA TTTTGATACA 
GGCAACAATG CAGGTGGTCC CACTCACCTC CCATTGGTGG CGCTGTCCCC CATCATTCAC 
CCTCGCCTCA GGGAGGTCCG CACTGGCCTG GGCTCCAGCG CACGCAAAGA CGTCCCTCAA 
TCCCCCATCT CTCTCCATGA TCGCTACAGC TCTCCAGCTG CTGGCAGCGC AAAGAGACGC 
TTGTTTGGTG ACGATGCTCC ACCACAATCC CCAGTAAAAA GGATCTCTGT CACTCCCATC 
AAAATCATTC CCAGTGGCTT GGAAAACCAG ACCACCACAA CCACTGTCCT CTCCATGGCC 
ACCGGAAATG GCCAGCAGCT CACCATTCCA CTGCCAGTGG TGAAGAATGA GGCAGGCGGC 
ATCACAGTCA TCCAGCTTCA GGCCAGCGAT ATGAATCCTC TCACTGCTCA AGTTTTATTG 
ACCGCTTCCC CGAGCCGGCC TGCTCTAGCC GCTCCAGCTA TCAGCTCTGA CTCACAGCCA 
CCACCTGCCA ATAAACCTCG CCGCACGGGC TCACTCGCAC TCTTCTTCAG GAAGGTGTAT 
CATTTAGCCA GTGTGCGTCT GCGAGACCTG TGTCTGAAGC TGGATATCTC CTCAGAGCTA 
AGAGGGAAGA TCTGGACGTG TTTTGAGCAC TCTCTCCTGC ACTGCACTGA CCTGATGAAG 
GACAGACACC TGGACCAGCT GCTGCTTTGT GCTGTTTACA TCATATCAAG GATCACGAAA 
GAAGAGCACA CTTTTCAGGA CATTATGAAA TGCTACCGAA CACAACCACA GGCCAACAGT 
CATGTGTACC GCAGTGTTTT AATAAAGAGG CGTCCCAAAG TGCAGCAGGC TGACGAGAAC 
ATGGAAGTCG ATCCTCCAGC AGACCAGTCC AATGAGAGGA GCGATCAGGC TGACAGAACA 
CAGACTGTCT TCGAATCAGA GGAAAGAGGG GATCTCATCC AGTTCTACAA CAACATTTAT 
GTGTCAAAGA TGAGATCATT CGCTTTCAAA TATGCACTTT CCACGCTTGA TAACAGGATG 
GAAGCTCCTC CGTTATCTCC GTTCCCCTCC GTTCGGTCTC ACACTCTCTC TCCTCGCCGT 
GTGTCTCAGA AACACTCCAT CTTTGTGTCT CCTCATAAAA ACAGCTCCAG TCTCACGCCC 
AGCACTGCAT ACACATACAG GTTCACTGGA AGCCCTTCAA AGGAGCTGAG TGACATAAAC 
CAGATGATCC GTCAGGGTGG CGTGAGCAAG AAGCGGGCGT TCACCATGGA GGGCGACGAG 
ACCGAATCTC CGTCTAAATG TCTCCGGCAA GAGAACGACG ACGTGCTGCT CAAACGCCTC 
CAGGACGTGG TCAGCGAGAG AGCCAGTCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

High-throughput genome editing and phenotyping facilitated by high resolution melting curve analysis.
PloS one9(12)e114632(2014)
Thomas HR,Percival SM,Yoder BK,Parant JM

Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish.
FEBS letters586(3)222-8(2012 Feb)
Hu SN,Yu H,Zhang YB,Wu ZL,Yan YC,Li YX,Li YY,Li YP

Coordinate control of cell cycle regulatory genes in zebrafish development tested by cyclin D1 knockdown with morpholino phosphorodiamidates and hydroxyprolyl-phosphono peptide nucleic acids.
Nucleic acids research33(15)4914-21(2005)
Duffy KT,McAleer MF,Davidson WR,Kari L,Kari C,Liu CG,Farber SA,Cheng KC,Mest JR,Wickstrom E,Dicker AP,Rodeck U

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rbl1 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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