EVPLB cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	EVPLB
Entrez Gene ID	793139
Full Name	envoplakin b
Synonyms	evpl,wu:fb17b09
General protein information	Preferred Names envoplakin b Names envoplakin
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001922038.7	XP_001922073.6	envoplakin
XM_001922038.6	XP_001922073.6	envoplakin

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EVPL	XP_003315788.1
Macaca mulatta (Rhesus monkey)	EVPL	XP_001101955.2
Canis lupus familiaris (dog)	EVPL	XP_540443.2
Mus musculus (house mouse)	Evpl	NP_079552.3
Xenopus tropicalis (tropical clawed frog)	evpl	NP_001128291.1
Homo sapiens (human)	EVPL	NP_001979.2
Gallus gallus (chicken)	EVPL	XP_004946182.1
Danio rerio (zebrafish)	evplb	XP_001922073.4
Bos taurus (cattle)	EVPL	NP_001192605.1
Rattus norvegicus (Norway rat)	Evpl	NP_001100536.1
Danio rerio (zebrafish)	evpla	NP_001112369.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EVPLB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EVPLB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa147044	XM_001922038.7 Latest version!	Danio rerio envoplakin b (evplb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa53478	XM_001922038.6 Latest version!	Danio rerio envoplakin b (evplb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EVPLB ( XM_001922038.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001922073.6 Danio rerio envoplakin b (evplb), mRNA.

Price & Availability↑

CloneID	ODa147044
Clone ID Related Accession (Same CDS sequence)	XM_001922038.7
Accession Version	XM_001922038.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	envoplakin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003334245.1) and transcript sequence (GFIL01009872.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001922038.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 974 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTTCAAGA AGAAGGAATA CAGCCCTGCT AAAGCCGGAC AGGCGCAGGT GAACGACCTG 
CTGTCTCTGG TCACACGCTT ACAGCAGAGC GCAGATCAGG TGGAGAAGAA CATCCTGCTG 
GCCGACAAAC TGCTGGAGGA GGATCTGGAG AATGAGCGCA GGGGCTCTCC TCTCAGACAG 
CAGGGTCCGG CCGCTGAGAA TCTGTCTCAG GCTGAGATGC TGCTGAAGGA TCTGTTCATG 
GACGTCAGCA AAGTCAAGAA GCTCCAGCAC CCGCAGGCCA GCATCATCGA GCGCGATGTG 
AGTAACCTGC ACGATCGTTG GTCCAAATAC TGCGGCACAT ACAGGCATCT GTACGGGCAG 
CTTCACGAGC TCTACCTGCC GCCCGGAGTG GACTGGAGCA AACTAATGAG CGACAAACAG 
AAACTCCTGC AGGACGAGGC TTACGGCCCG ACTCTGACTG ATGTGGAGAA GCAGATCGCA 
CAACACAACA TCCTGCACAA GCAGATAGAG GAGTACCACA GAGCCCTGGA GGACAGCGGA 
GATAACTCGT CTGCTCTTCA GAAGCAGTAT GCGGCTCTGC TGGCGTCATC AGCAGGTCGT 
CGTGCGCATC TGTCGTCGCT GTATGAGCTG ATGCAGGCCT GCAGTAGAGA GCTGCAGTAT 
CTCAGTGAGC AGCAGCAGAA GATCATCAGC AGAGACTGGA GCGACCGCAT CATCGACAGC 
GCTGGAGTGC GCATGGAGAA CGAGAGGTTT AAGAACAACG GTCTGCTGAC ACACGAGAGA 
GAAGTGAGCA AACTACAGGA GGAGGCCAAC GCTCTCATTC AGGACAAACA CCCCGGCGCA 
GCCGCTGTCA AGTCGTACAG TGCAGACGTC ACAAGAGAAT GGAAACAATT CCTCAGTTTG 
TGTCTGTGTC AGGACACACA CCTGGACAAC GTGGAGGAGT ACAAGAAATA TCAGCTGAAT 
CTGGAGACGT TCACTGAGTC GCTGAATAGA ATCAAATCCA CCACTGAACC ATCAGCTCTG 
AGAAACATGA GCAATCCTGA GATCCTGCTG CAGCTCGAGG CGGAGGAGCG GTCGATGCTG 
AGGAACGAGC AGCGTCTGGC GGACCTGAGA GAGTTGAGCT CCTCCATCGC TCCTCTACAT 
CTGCGCAGAT CCACCACCGG CAGATCCATC AGCGCCGTCG CTCTGTGTGA CTGGACCACC 
GACAAGGACT CGGTAAAGCG CGGTGAGAAG GTAACACTGG TCTCCAGCCC CAGAGAGCAG 
ATGTGGGAGG TCAGTTCCAG CAGCGGAGGT ACCAAACTGA TCCCTGGAGC CTGTTTCATG 
ATCCCTCCGC CTGATTCTGC TGCCATCGAC AAGGTGGACA GCCTGAGCAG AGATTTCTCG 
GATCTGAAGA GGACCAGATC GGCTCTACAG GCTCAGGCCA AAACACCCAA CATTGAAGTG 
GTGCAGTTCG TCAAACAAGT CGCTGTGGCC ACTGTTCCTG ACAGTGCAAA GGCCAGTGTC 
ATCGACAGCC GTCTGGACCA GATCTACTCA GACCTGCTGA AGCTGGAGAA AGACATGCAG 
AACCGCATCA GAACTCCACT GGACCGCTCC GCGCCTGTCG GAGACCTGTC TGCCAGAATC 
AAAGACCAGG AGAGTTTTGC ATTGGTTCTG AAGAATCTGG AGGCAGAGAA AGCTTCAGCT 
CAGAGGGACA TGCAACCCCT GATCTCCCAG AAGCCCCTGG GGCCCACCAC ATCCTCGCTG 
CCTCTGAAAC TGAGCAAAGT CAACAACAAG ATTGATGACA TCAGCGCACT CTCCGACCTA 
TACCACAAGA AGGCCACGTC TGCCATGTTC CTGGAGAAGC AGATCAGGAT GGTGGACGGT 
GTGCTGACGG ACTTCGAGGA TCAGCTGTGT GCAGATTCGG GGCTGTTAGA CGAAGACAAT 
GCCATTCGCA ACCACTCCAG ACAACTGCAG CAATTGAGTA AGGACGTTGC ATCCAGGAAG 
GGAGACATTC AGCAGCTGAA CGGTGAGCTG GAGGCCACCG AGCAGACGTG CAGCTCCCTG 
CAGAAGAGTT TTCAGGAGTT CTGCCCGGAT ATCCGCCATC AGGAGAGCCA AGTCAAGCGC 
TTGAGGAACC GATACTCCAA CATCAACAGC CAGCTGCAGC AGAGGGCAAC TCTGTTGAAA 
GAAGCTGTCA GTAAGTACCA GGAGTTTCAG AGCGTCAACC AATCGCTCAC TGCTTTCCTC 
ATCAACATGC CCAACAACAA GGTGCAGCCT GGTGAAAGTA TGACGCAAGT CTATGCCAAG 
CAAAACTCGC AGATGAGAGC AGTTGAAGAC ATCAAGAGGA AGCAGGACGA TGTGAACAGG 
ATCGTGAAAC TTTCCGAGGA GCTGCAGGGC GTTCTCAATG AGTATGAGGT CAATTCTAAC 
AAATACCGCA GTGCATTGGG AAAGCCGGAA ATTAATTCAA AAGGACTTGA TCACTTAACC 
CTGGCTGAAT TTGTGAAGAA GAAGGAAAAG GATGTTGTGA ATCTATACTC TGAAGTATCT 
GCTGAGAATA ACCAGCTTCT GAATCAACTG TACTTGGCCA AAGATCTCAT CTACAAGAAT 
GAAGAAATCG TGAACCATGT GGTCGTAAAG CAGCAACTGC AGATGCAAAG TCAGCAGAGA 
GAAATGGAGG AAGTTGACGG CTTGAAGCAA GAGCTTTTTG AGGAAATATC TCGTCGTACA 
CATGCTGAGA ATGATCTATT TGCCCAAAGG AAACGACTGG TGTCCCTGAA GAGTCGCCGA 
GGTGTGGAGC GAGTGGAGGA AAAGGAAACA GTGCATTACT ACCGTGATCC GAAGATTGAG 
GCTGACTTGG AGGTGCTAAA GAAGAGGATA GCAGACGAGA TCTACAAGCG ATCCGAAATG 
CAATCCGAAA TTGAGATTTT CAAGCAAAAA ATTGTCAGCC GTGAGCGAGA TGTTGTAGAA 
GTAAAGCCCA GGTTGGTGAC AAAGGTGGTT ACAGAAATTG AGCGTGACCC CAAATTAGAT 
GTGGAAGCAG CGAGGATTAG GGAGCTCATA CTTAAACTAA AAGAGGAAAT TCGAGTGCTT 
GAGAGTAATT CAGTTAACAT CCGAACGGAG GTAACTGTCC TGGAGAACAA GAAGCCTGTG 
ATAAAGGAAA GGGTCATCCT TAAAGAGGTT GTGAGGCTGG AAAAGGACCC AGAAATGCTG 
AAAACTGTAA TGAGCTTCAA GAGCGAGATA TCGGATGAGA GTATCAGATG CAAGAACCTG 
AATGATGAAA TATTCGTTAT TAGGAGTCAG ATCAATAACT TGGAGAGAAT TATTCCAACC 
ATACAACCCA AAACTATCAT CAAAGAGGTA AAGAGGGTGG AACAAGATGC TGACCTCCTA 
CAAGAGTCCA GAACTCTTCG TGTGACCCTG GAAGAGCTGA TCCAAGAGTA CAGTATTTTG 
ATGAAAGAGA TCTCCAGCCT TCAGGTCCGC TACTCCCAAG TAGAAACCAT CAGACAGAAG 
ATCGAGTTCA ATGAGATCAT TCATGAGATC TACAGGATTG ATCCTGAGAC GGAGAGGGAG 
CTTCGACGGC TGAGAAACGA ACTTATCGAA ATAGGCAAAC GCCGCACTAG CATTGAAAGT 
GAGATTAGTT TAATTATGGT GGATCTGAAG ACTCTGCGTG CCGAGAAACC CAAAGTTGAG 
CTGAAGGAGG TGACTCGAGA GGTTGTGAAA GAAGAGAGGA GCCCTGAGAT TATCAGAGAA 
ATTAAGAGGC TGAATGAGCA GCTTACCATT TTGCGTACCA ACTACGATTC TACTACGAGT 
CGTGTGTTTC GTCTGCGCAA GGACAGGGAT GAGTGGAAGG CTGAAAGATC CAAGGTGGAA 
ACGAAAGTGG TGACCAAAGA GTTGATAAGG TATGAAAATG ACCCTCTTCT GGAGAAAGAA 
GCTGAACGTC TTAGGAAGGA AGTCAGAGAG GAAATTCAAT GCCGCCGAAC AGTGGAAGAG 
AATGTGTTTG ATCTTCAAAA CAAGTACATT GTGCTCGAGA GACAGAAACC AGAGGAAAGA 
GTTGTGGTCC AAGAAATAGT GCGTCTCCAG ACAGATCCGA GTCAGATTCA GACTCATGAG 
AGATTAGTCA GGACTTTGGA AGAGACTATC AAGGTTCGAC GACAGCTAGA ACTGGAGGTG 
CAGCAGATGA GATCTGTAAT CCTGGAACTG GAGAAAAAAC TGGAGCTTGT GGATCGACAG 
AAGAAGGTAA TGGTTGAGAC AGAGTTGAGA CAAATCAAGG CTAGAATCTA TGAACTTGAG 
AACTGCCCAA AACCTGTTGA GGAAAAGATC GTCATTGAGG AGGTTTTGAA AGTTGAGAGA 
GACTCTAAAG TCGACAAACT TATCACTGGG CTTCGCACTG AGATTGACAA TGAGGGTGCC 
AGCATTTCAA GACTGGAGAG AGAGATCCGA AACCTCAGAA TTAGGATTGA GTCCCTGACC 
AAGGAGAAAT CTGTTGAGAG GATCATCCAC AAAGAGGTGA TCAGGGTTGA GAAGGACCAA 
GCAGTGGAGG CAGAGAGAGA GAGCCTTCTG ACCCAAGTCT CTCAAGTGAA GAGTACCAGG 
ACCCTTAAAG ATGAAGAACT GCTGAGATTG AAAAACAAAA TCACCCGAAT TCAGACCACC 
AGGACAAACT TCTCTCAAGA GGAGACCACC TTGATTGCCA ACAGGGATAG GATCAAGAGG 
GAGCAAAACG AACTTCTCCA GGAGCTGAAA AGACTGGAGA CTCAAAAGCA GGAGATCAGC 
ATAACTTTCC AACAACAGAC CAAACTCCTG AGCGAGAGGA ACCAACTCAG CAGACAAAAG 
AGCATCAAGA TGGAGTCAGA GATCCAGCGC CTGGAAAGGG AAATCCTGGA GGTGAAAGAA 
CTGATCAACG TTCGGGAAAA AACTCTCATC GAGCTCCAAA AAGAGGCAAA GTCTGTGCAG 
AATACAGAGA CAAACACCAG AGAAACTAAC GTCTCTACCA AAATCACCAT TCTGGATCCA 
GAGACTGGCA AAGACATGTC TCCATACGAC GCCTATCTTG AGGGCCTGAT TGACCGCAAT 
CAGTACATCC ACCTGCAGGA GCTGGAGTGC AGCTGGGAAG AAATCACAAC CACGGGACCT 
GACGGTGAAG TCACACTCCT GCAAGATCGT AAAAGTGGAA AGCAGTACTC CATCAAGACG 
GCTCTCCAAA ATGGACGACT AACCCAATAT GACCTTCAGC GCTACAGAGA TGGTAAAATG 
ACCATTTCGG AGTTCGCTCT CTTGGTTGCA GGAGAAAAAA AATCAACAGT CTTTCCTCCC 
TCGAGTGTAA CACAAACAAC ATCCCTTCAG ACAAACTCCA GCTACAGCCC CTCAACACTG 
ACACGGCGTA CATCCTACTC CAGCAACGGC AACTTAAACT CTCTGAACAC AAGCGGAGAT 
GAGCTCTACC CTATTTCTGG CATTCTGGAC ATCACCACAG AAAGCCGAAT GTCTGTAAGA 
AGTGCCCTAA CACGCAAACT GATTGATCAA GACACCGCAA TGAGGCTCCT CGAAGCTCAG 
GCTGCTACTG GAGGAATTGT GGACCTCAAT AAGAAAGACA AGCTGTCCGT TCATAAGGCT 
GCAGAGCGTG GATTGATCGA CTCCAGCCAA CTTCACAAAC TCCTCAGTGC CCAAAAGGCA 
TTCACTGGGT TTGAAGACCC TGTGTCTAAA GAGCGCATGT CAGTGGCAGA AGCCGCTCGT 
AGAGGATGGA TCCCAAATGA TAGTGCTATA TGGTTTATGG AGGCACAGCT GTTAACCGGT 
GGCCTTGTTG ACCCAAACCG AGCAGGTCGC ATCGGTCTGC AAAACGCAAT TGATGCAAAA 
TTGATCGACA CATCGATGGC CAAGCAACTC CAAGATGATC TGGCCTTAGT GAAGAACGTT 
GTGGATCCCG TAAAAAAGGA TAAAATCACA TATAAAGAGG CCATGGCTCG ATGCAAGCGG 
GATCACAGCA CGGGACTGCT CCTGTTTCCG GCAGCCTCCA CTGACACCGT AGACGCTCCG 
TCCTATTCCA GCTACAAATT CACCAAGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001922073.6
CDS	123..6155
Translation	MFKKKEYSPAKAGQAQVNDLLSLVTRLQQSADQVEKNILLADKLLEEDLENERRGSPLRQQGPAAENLSQAEMLLKDLFMDVSKVKKLQHPQASIIERDVSNLHDRWSKYCGTYRHLYGQLHELYLPPGVDWSKLMSDKQKLLQDEAYGPTLTDVEKQIAQHNILHKQIEEYHRALEDSGDNSSALQKQYAALLASSAGRRAHLSSLYELMQACSRELQYLSEQQQKIISRDWSDRIIDSAGVRMENERFKNNGLLTHEREVSKLQEEANALIQDKHPGAAAVKSYSADVTREWKQFLSLCLCQDTHLDNVEEYKKYQLNLETFTESLNRIKSTTEPSALRNMSNPEILLQLEAEERSMLRNEQRLADLRELSSSIAPLHLRRSTTGRSISAVALCDWTTDKDSVKRGEKVTLVSSPREQMWEVSSSSGGTKLIPGACFMIPPPDSAAIDKVDSLSRDFSDLKRTRSALQAQAKTPNIEVVQFVKQVAVATVPDSAKASVIDSRLDQIYSDLLKLEKDMQNRIRTPLDRSAPVGDLSARIKDQESFALVLKNLEAEKASAQRDMQPLISQKPLGPTTSSLPLKLSKVNNKIDDISALSDLYHKKATSAMFLEKQIRMVDGVLTDFEDQLCADSGLLDEDNAIRNHSRQLQQLSKDVASRKGDIQQLNGELEATEQTCSSLQKSFQEFCPDIRHQESQVKRLRNRYSNINSQLQQRATLLKEAVSKYQEFQSVNQSLTAFLINMPNNKVQPGESMTQVYAKQNSQMRAVEDIKRKQDDVNRIVKLSEELQGVLNEYEVNSNKYRSALGKPEINSKGLDHLTLAEFVKKKEKDVVNLYSEVSAENNQLLNQLYLAKDLIYKNEEIVNHVVVKQQLQMQSQQREMEEVDGLKQELFEEISRRTHAENDLFAQRKRLVSLKSRRGVERVEEKETVHYYRDPKIEADLEVLKKRIADEIYKRSEMQSEIEIFKQKIVSRERDVVEVKPRLVTKVVTEIERDPKLDVEAARIRELILKLKEEIRVLESNSVNIRTEVTVLENKKPVIKERVILKEVVRLEKDPEMLKTVMSFKSEISDESIRCKNLNDEIFVIRSQINNLERIIPTIQPKTIIKEVKRVEQDADLLQESRTLRVTLEELIQEYSILMKEISSLQVRYSQVETIRQKIEFNEIIHEIYRIDPETERELRRLRNELIEIGKRRTSIESEISLIMVDLKTLRAEKPKVELKEVTREVVKEERSPEIIREIKRLNEQLTILRTNYDSTTSRVFRLRKDRDEWKAERSKVETKVVTKELIRYENDPLLEKEAERLRKEVREEIQCRRTVEENVFDLQNKYIVLERQKPEERVVVQEIVRLQTDPSQIQTHERLVRTLEETIKVRRQLELEVQQMRSVILELEKKLELVDRQKKVMVETELRQIKARIYELENCPKPVEEKIVIEEVLKVERDSKVDKLITGLRTEIDNEGASISRLEREIRNLRIRIESLTKEKSVERIIHKEVIRVEKDQAVEAERESLLTQVSQVKSTRTLKDEELLRLKNKITRIQTTRTNFSQEETTLIANRDRIKREQNELLQELKRLETQKQEISITFQQQTKLLSERNQLSRQKSIKMESEIQRLEREILEVKELINVREKTLIELQKEAKSVQNTETNTRETNVSTKITILDPETGKDMSPYDAYLEGLIDRNQYIHLQELECSWEEITTTGPDGEVTLLQDRKSGKQYSIKTALQNGRLTQYDLQRYRDGKMTISEFALLVAGEKKSTVFPPSSVTQTTSLQTNSSYSPSTLTRRTSYSSNGNLNSLNTSGDELYPISGILDITTESRMSVRSALTRKLIDQDTAMRLLEAQAATGGIVDLNKKDKLSVHKAAERGLIDSSQLHKLLSAQKAFTGFEDPVSKERMSVAEAARRGWIPNDSAIWFMEAQLLTGGLVDPNRAGRIGLQNAIDAKLIDTSMAKQLQDDLALVKNVVDPVKKDKITYKEAMARCKRDHSTGLLLFPAASTDTVDAPSYSSYKFTKK

Target ORF information:

RefSeq Version	XM_001922038.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio envoplakin b (evplb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922038.7

ORF Insert Sequence:

ATGTTCAAGA AGAAGGAATA CAGCCCTGCT AAAGCCGGAC AGGCGCAGGT GAACGACCTG 
CTGTCTCTGG TCACACGCTT ACAGCAGAGC GCAGATCAGG TGGAGAAGAA CATCCTGCTG 
GCCGACAAAC TGCTGGAGGA GGATCTGGAG AATGAGCGCA GGGGCTCTCC TCTCAGACAG 
CAGGGTCCGG CCGCTGAGAA TCTGTCTCAG GCTGAGATGC TGCTGAAGGA TCTGTTCATG 
GACGTCAGCA AAGTCAAGAA GCTCCAGCAC CCGCAGGCCA GCATCATCGA GCGCGATGTG 
AGTAACCTGC ACGATCGTTG GTCCAAATAC TGCGGCACAT ACAGGCATCT GTACGGGCAG 
CTTCACGAGC TCTACCTGCC GCCCGGAGTG GACTGGAGCA AACTAATGAG CGACAAACAG 
AAACTCCTGC AGGACGAGGC TTACGGCCCG ACTCTGACTG ATGTGGAGAA GCAGATCGCA 
CAACACAACA TCCTGCACAA GCAGATAGAG GAGTACCACA GAGCCCTGGA GGACAGCGGA 
GATAACTCGT CTGCTCTTCA GAAGCAGTAT GCGGCTCTGC TGGCGTCATC AGCAGGTCGT 
CGTGCGCATC TGTCGTCGCT GTATGAGCTG ATGCAGGCCT GCAGTAGAGA GCTGCAGTAT 
CTCAGTGAGC AGCAGCAGAA GATCATCAGC AGAGACTGGA GCGACCGCAT CATCGACAGC 
GCTGGAGTGC GCATGGAGAA CGAGAGGTTT AAGAACAACG GTCTGCTGAC ACACGAGAGA 
GAAGTGAGCA AACTACAGGA GGAGGCCAAC GCTCTCATTC AGGACAAACA CCCCGGCGCA 
GCCGCTGTCA AGTCGTACAG TGCAGACGTC ACAAGAGAAT GGAAACAATT CCTCAGTTTG 
TGTCTGTGTC AGGACACACA CCTGGACAAC GTGGAGGAGT ACAAGAAATA TCAGCTGAAT 
CTGGAGACGT TCACTGAGTC GCTGAATAGA ATCAAATCCA CCACTGAACC ATCAGCTCTG 
AGAAACATGA GCAATCCTGA GATCCTGCTG CAGCTCGAGG CGGAGGAGCG GTCGATGCTG 
AGGAACGAGC AGCGTCTGGC GGACCTGAGA GAGTTGAGCT CCTCCATCGC TCCTCTACAT 
CTGCGCAGAT CCACCACCGG CAGATCCATC AGCGCCGTCG CTCTGTGTGA CTGGACCACC 
GACAAGGACT CGGTAAAGCG CGGTGAGAAG GTAACACTGG TCTCCAGCCC CAGAGAGCAG 
ATGTGGGAGG TCAGTTCCAG CAGCGGAGGT ACCAAACTGA TCCCTGGAGC CTGTTTCATG 
ATCCCTCCGC CTGATTCTGC TGCCATCGAC AAGGTGGACA GCCTGAGCAG AGATTTCTCG 
GATCTGAAGA GGACCAGATC GGCTCTACAG GCTCAGGCCA AAACACCCAA CATTGAAGTG 
GTGCAGTTCG TCAAACAAGT CGCTGTGGCC ACTGTTCCTG ACAGTGCAAA GGCCAGTGTC 
ATCGACAGCC GTCTGGACCA GATCTACTCA GACCTGCTGA AGCTGGAGAA AGACATGCAG 
AACCGCATCA GAACTCCACT GGACCGCTCC GCGCCTGTCG GAGACCTGTC TGCCAGAATC 
AAAGACCAGG AGAGTTTTGC ATTGGTTCTG AAGAATCTGG AGGCAGAGAA AGCTTCAGCT 
CAGAGGGACA TGCAACCCCT GATCTCCCAG AAGCCCCTGG GGCCCACCAC ATCCTCGCTG 
CCTCTGAAAC TGAGCAAAGT CAACAACAAG ATTGATGACA TCAGCGCACT CTCCGACCTA 
TACCACAAGA AGGCCACGTC TGCCATGTTC CTGGAGAAGC AGATCAGGAT GGTGGACGGT 
GTGCTGACGG ACTTCGAGGA TCAGCTGTGT GCAGATTCGG GGCTGTTAGA CGAAGACAAT 
GCCATTCGCA ACCACTCCAG ACAACTGCAG CAATTGAGTA AGGACGTTGC ATCCAGGAAG 
GGAGACATTC AGCAGCTGAA CGGTGAGCTG GAGGCCACCG AGCAGACGTG CAGCTCCCTG 
CAGAAGAGTT TTCAGGAGTT CTGCCCGGAT ATCCGCCATC AGGAGAGCCA AGTCAAGCGC 
TTGAGGAACC GATACTCCAA CATCAACAGC CAGCTGCAGC AGAGGGCAAC TCTGTTGAAA 
GAAGCTGTCA GTAAGTACCA GGAGTTTCAG AGCGTCAACC AATCGCTCAC TGCTTTCCTC 
ATCAACATGC CCAACAACAA GGTGCAGCCT GGTGAAAGTA TGACGCAAGT CTATGCCAAG 
CAAAACTCGC AGATGAGAGC AGTTGAAGAC ATCAAGAGGA AGCAGGACGA TGTGAACAGG 
ATCGTGAAAC TTTCCGAGGA GCTGCAGGGC GTTCTCAATG AGTATGAGGT CAATTCTAAC 
AAATACCGCA GTGCATTGGG AAAGCCGGAA ATTAATTCAA AAGGACTTGA TCACTTAACC 
CTGGCTGAAT TTGTGAAGAA GAAGGAAAAG GATGTTGTGA ATCTATACTC TGAAGTATCT 
GCTGAGAATA ACCAGCTTCT GAATCAACTG TACTTGGCCA AAGATCTCAT CTACAAGAAT 
GAAGAAATCG TGAACCATGT GGTCGTAAAG CAGCAACTGC AGATGCAAAG TCAGCAGAGA 
GAAATGGAGG AAGTTGACGG CTTGAAGCAA GAGCTTTTTG AGGAAATATC TCGTCGTACA 
CATGCTGAGA ATGATCTATT TGCCCAAAGG AAACGACTGG TGTCCCTGAA GAGTCGCCGA 
GGTGTGGAGC GAGTGGAGGA AAAGGAAACA GTGCATTACT ACCGTGATCC GAAGATTGAG 
GCTGACTTGG AGGTGCTAAA GAAGAGGATA GCAGACGAGA TCTACAAGCG ATCCGAAATG 
CAATCCGAAA TTGAGATTTT CAAGCAAAAA ATTGTCAGCC GTGAGCGAGA TGTTGTAGAA 
GTAAAGCCCA GGTTGGTGAC AAAGGTGGTT ACAGAAATTG AGCGTGACCC CAAATTAGAT 
GTGGAAGCAG CGAGGATTAG GGAGCTCATA CTTAAACTAA AAGAGGAAAT TCGAGTGCTT 
GAGAGTAATT CAGTTAACAT CCGAACGGAG GTAACTGTCC TGGAGAACAA GAAGCCTGTG 
ATAAAGGAAA GGGTCATCCT TAAAGAGGTT GTGAGGCTGG AAAAGGACCC AGAAATGCTG 
AAAACTGTAA TGAGCTTCAA GAGCGAGATA TCGGATGAGA GTATCAGATG CAAGAACCTG 
AATGATGAAA TATTCGTTAT TAGGAGTCAG ATCAATAACT TGGAGAGAAT TATTCCAACC 
ATACAACCCA AAACTATCAT CAAAGAGGTA AAGAGGGTGG AACAAGATGC TGACCTCCTA 
CAAGAGTCCA GAACTCTTCG TGTGACCCTG GAAGAGCTGA TCCAAGAGTA CAGTATTTTG 
ATGAAAGAGA TCTCCAGCCT TCAGGTCCGC TACTCCCAAG TAGAAACCAT CAGACAGAAG 
ATCGAGTTCA ATGAGATCAT TCATGAGATC TACAGGATTG ATCCTGAGAC GGAGAGGGAG 
CTTCGACGGC TGAGAAACGA ACTTATCGAA ATAGGCAAAC GCCGCACTAG CATTGAAAGT 
GAGATTAGTT TAATTATGGT GGATCTGAAG ACTCTGCGTG CCGAGAAACC CAAAGTTGAG 
CTGAAGGAGG TGACTCGAGA GGTTGTGAAA GAAGAGAGGA GCCCTGAGAT TATCAGAGAA 
ATTAAGAGGC TGAATGAGCA GCTTACCATT TTGCGTACCA ACTACGATTC TACTACGAGT 
CGTGTGTTTC GTCTGCGCAA GGACAGGGAT GAGTGGAAGG CTGAAAGATC CAAGGTGGAA 
ACGAAAGTGG TGACCAAAGA GTTGATAAGG TATGAAAATG ACCCTCTTCT GGAGAAAGAA 
GCTGAACGTC TTAGGAAGGA AGTCAGAGAG GAAATTCAAT GCCGCCGAAC AGTGGAAGAG 
AATGTGTTTG ATCTTCAAAA CAAGTACATT GTGCTCGAGA GACAGAAACC AGAGGAAAGA 
GTTGTGGTCC AAGAAATAGT GCGTCTCCAG ACAGATCCGA GTCAGATTCA GACTCATGAG 
AGATTAGTCA GGACTTTGGA AGAGACTATC AAGGTTCGAC GACAGCTAGA ACTGGAGGTG 
CAGCAGATGA GATCTGTAAT CCTGGAACTG GAGAAAAAAC TGGAGCTTGT GGATCGACAG 
AAGAAGGTAA TGGTTGAGAC AGAGTTGAGA CAAATCAAGG CTAGAATCTA TGAACTTGAG 
AACTGCCCAA AACCTGTTGA GGAAAAGATC GTCATTGAGG AGGTTTTGAA AGTTGAGAGA 
GACTCTAAAG TCGACAAACT TATCACTGGG CTTCGCACTG AGATTGACAA TGAGGGTGCC 
AGCATTTCAA GACTGGAGAG AGAGATCCGA AACCTCAGAA TTAGGATTGA GTCCCTGACC 
AAGGAGAAAT CTGTTGAGAG GATCATCCAC AAAGAGGTGA TCAGGGTTGA GAAGGACCAA 
GCAGTGGAGG CAGAGAGAGA GAGCCTTCTG ACCCAAGTCT CTCAAGTGAA GAGTACCAGG 
ACCCTTAAAG ATGAAGAACT GCTGAGATTG AAAAACAAAA TCACCCGAAT TCAGACCACC 
AGGACAAACT TCTCTCAAGA GGAGACCACC TTGATTGCCA ACAGGGATAG GATCAAGAGG 
GAGCAAAACG AACTTCTCCA GGAGCTGAAA AGACTGGAGA CTCAAAAGCA GGAGATCAGC 
ATAACTTTCC AACAACAGAC CAAACTCCTG AGCGAGAGGA ACCAACTCAG CAGACAAAAG 
AGCATCAAGA TGGAGTCAGA GATCCAGCGC CTGGAAAGGG AAATCCTGGA GGTGAAAGAA 
CTGATCAACG TTCGGGAAAA AACTCTCATC GAGCTCCAAA AAGAGGCAAA GTCTGTGCAG 
AATACAGAGA CAAACACCAG AGAAACTAAC GTCTCTACCA AAATCACCAT TCTGGATCCA 
GAGACTGGCA AAGACATGTC TCCATACGAC GCCTATCTTG AGGGCCTGAT TGACCGCAAT 
CAGTACATCC ACCTGCAGGA GCTGGAGTGC AGCTGGGAAG AAATCACAAC CACGGGACCT 
GACGGTGAAG TCACACTCCT GCAAGATCGT AAAAGTGGAA AGCAGTACTC CATCAAGACG 
GCTCTCCAAA ATGGACGACT AACCCAATAT GACCTTCAGC GCTACAGAGA TGGTAAAATG 
ACCATTTCGG AGTTCGCTCT CTTGGTTGCA GGAGAAAAAA AATCAACAGT CTTTCCTCCC 
TCGAGTGTAA CACAAACAAC ATCCCTTCAG ACAAACTCCA GCTACAGCCC CTCAACACTG 
ACACGGCGTA CATCCTACTC CAGCAACGGC AACTTAAACT CTCTGAACAC AAGCGGAGAT 
GAGCTCTACC CTATTTCTGG CATTCTGGAC ATCACCACAG AAAGCCGAAT GTCTGTAAGA 
AGTGCCCTAA CACGCAAACT GATTGATCAA GACACCGCAA TGAGGCTCCT CGAAGCTCAG 
GCTGCTACTG GAGGAATTGT GGACCTCAAT AAGAAAGACA AGCTGTCCGT TCATAAGGCT 
GCAGAGCGTG GATTGATCGA CTCCAGCCAA CTTCACAAAC TCCTCAGTGC CCAAAAGGCA 
TTCACTGGGT TTGAAGACCC TGTGTCTAAA GAGCGCATGT CAGTGGCAGA AGCCGCTCGT 
AGAGGATGGA TCCCAAATGA TAGTGCTATA TGGTTTATGG AGGCACAGCT GTTAACCGGT 
GGCCTTGTTG ACCCAAACCG AGCAGGTCGC ATCGGTCTGC AAAACGCAAT TGATGCAAAA 
TTGATCGACA CATCGATGGC CAAGCAACTC CAAGATGATC TGGCCTTAGT GAAGAACGTT 
GTGGATCCCG TAAAAAAGGA TAAAATCACA TATAAAGAGG CCATGGCTCG ATGCAAGCGG 
GATCACAGCA CGGGACTGCT CCTGTTTCCG GCAGCCTCCA CTGACACCGT AGACGCTCCG 
TCCTATTCCA GCTACAAATT CACCAAGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVPLB ( XM_001922038.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001922073.6 Danio rerio envoplakin b (evplb), mRNA.

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CloneID	ODa53478
Clone ID Related Accession (Same CDS sequence)	XM_001922038.6
Accession Version	XM_001922038.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	envoplakin
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003334245.1) and transcript sequence (GDQH01003003.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001922038.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1016 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTTCAAGA AGAAGGAATA CAGCCCTGCT AAAGCCGGAC AGGCGCAGGT GAACGACCTG 
CTGTCTCTGG TCACACGCTT ACAGCAGAGC GCAGATCAGG TGGAGAAGAA CATCCTGCTG 
GCCGACAAAC TGCTGGAGGA GGATCTGGAG AATGAGCGCA GGGGCTCTCC TCTCAGACAG 
CAGGGTCCGG CCGCTGAGAA TCTGTCTCAG GCTGAGATGC TGCTGAAGGA TCTGTTCATG 
GACGTCAGCA AAGTCAAGAA GCTCCAGCAC CCGCAGGCCA GCATCATCGA GCGCGATGTG 
AGTAACCTGC ACGATCGTTG GTCCAAATAC TGCGGCACAT ACAGGCATCT GTACGGGCAG 
CTTCACGAGC TCTACCTGCC GCCCGGAGTG GACTGGAGCA AACTGATGAG TGACAAACAG 
AAACTCCTGC AGGACGAGGC TTACGGCCCG ACTCTGACTG ATGTGGAGAA GCAGATCGCA 
CAACACAACA TCCTGCACAA GCAGATAGAG GAGTACCACA GAGCCCTGGA GGACAGCGGA 
GATAACTCGT CTGCTCTTCA GAAGCAGTAT GCGGCTCTGC TGGCGTCATC AGCAGGTCGT 
CGTGCGCATC TGTCGTCGCT GTATGAGCTG ATGCAGGCCT GCAGTAGAGA GCTGCAGTAT 
CTCAGTGAGC AGCAGCAGAA GATCATCAGC AGAGACTGGA GCGACCGCAT CATCGACAGC 
GCTGGAGTGC GCATGGAGAA CGAGAGGTTT AAGAACAACG GTCTGCTCAC ACACGAAAGA 
GAAGTGAGCA AACTACAGGA GGAGGCCAAC GCTCTCATTC AGGACAAACA CCCTGGCGCA 
GCTGCTGTCA AGTCGTACAG TGCAGACGTC ACAAGAGAAT GGAAACAATT CCTCAGTTTG 
TGTCTGTGTC AGGACACACA CCTGGACAAC GTGGAGGAGT ACAAGAAATA TCAGCTGAAT 
CTGGAGACGT TCACTGAGTC GCTGAATAGA ATCAAATCCA CCACTGAACC ATCAGCTCTG 
AGAAACATGA GCAATCCTGA GATCCTGCTG CAGCTCGAGG CGGAGGAGCG GTCGATGCTG 
AGGAACGAGC AGCGTCTGGC GGACCTGAGA GAGTTGAGCT CCTCCATCGC TCCTCTACAT 
CTGCGCAGAT CCACCACCGG CAGATCCATC AGCGCCGTCG CTCTGTGTGA CTGGACCACC 
GACAAGGACT CGGTAAAGCG CGGTGAGAAG GTAACACTGG TCTCCAGCCC CAGAGAGCAG 
ATGTGGGAGG TCAGTTCCAG CAGCGGAGGT ACCAAACTGA TCCCTGGAGC CTGTTTCATG 
ATCCCTCCGC CTGATTCTGC TGCCATCGAC AAGGTGGACA GCCTGAGCAG AGATTTCTCG 
GATCTGAAGA GGACCAGATC GGCTCTACAG GCTCAGGCCA AAACACCCAA CATTGAAGTG 
GTGCAGTTCG TCAAACAAGT CGCTGTGGCC ACTGTTCCTG ACAGTGCAAA GGCCAGTGTC 
ATCGACAGCC GTCTGGACCA GATCTACTCA GACCTGCTGA AGCTGGAGAA AGACATGCAG 
AACCGCATCA GAACTCCACT GGACCGCTCC GCGCCTGTCG GAGACCTGTC TGCCAGAATC 
AAAGACCAGG AGAGTTTTGC ATTGGTTCTG AAGAATCTGG AGGCAGAGAA AGCTTCAGCT 
CAGAGGGACA TGCAACCCCT GATCTCCCAG AAGCCCCTGG GGCCCACCAC ATCCTCGCTG 
CCTCTGAAAC TGAGCAAAGT CAACAACAAG ATTGATGACA TCAGCGCACT CTCCGACCTA 
TACCACAAGA AGGCCACGTC TGCCATGTTC CTGGAGAAGC AGATCAGGAT GGTGGACGGT 
GTGCTGACGG ACTTCGAGGA TCAGCTGTGT GCAGATTCGG GGCTGTTAGA CGAAGACAAT 
GCCATTCGCA ACCACTCCAG ACAACTGCAG CAATTGAGTA AGGACGTTGC ATCCAGGAAG 
GGAGACATTC AGCAGCTGAA CGGTGAGCTG GAGGCCACCG AGCAGACGTG CAGCTCCCTG 
CAGAAGAGTT TTCAGGAGTT CTGCCCGGAT ATCCGCCATC AGGAGAGCCA AGTCAAGCGC 
TTGAGGAACC GATACTCCAA CATCAACAGC CAGCTGCAGC AGAGGGCAAC TCTGTTGAAA 
GAAGCTGTCA GTAAGTACCA GGAGTTTCAG AGCGTCAACC AATCGCTCAC TGCTTTCCTC 
ATCAACATGC CCAACAACAA GGTGCAGCCT GGTGAAAGTA TGACGCAAGT CTATGCCAAG 
CAAAACTCGC AGATGAGAGC AGTTGAAGAC ATCAAGAGGA AGCAGGACGA TGTGAACAGG 
ATCGTGAAAC TTTCCGAGGA GCTGCAGGGC GTTCTCAATG AGTATGAGGT CAATTCTAAC 
AAATACCGCA GTGCATTGGG AAAGCCGGAA ATTAATTCAA AAGGACTTGA TCACTTAACC 
CTGGCTGAAT TTGTGAAGAA GAAGGAAAAG GATGTTGTGA ATCTATACTC TGAAGTATCT 
GCTGAGAATA ACCAGCTTCT GAATCAACTG TACTTGGCCA AAGATCTCAT CTACAAGAAT 
GAAGAAATCG TGAACCATGT GGTCGTAAAG CAGCAACTGC AGATGCAAAG TCAGCAGAGA 
GAAATGGAGG AAGTTGACGG CTTGAAGCAA GAGCTTTTTG AGGAAATATC TCGTCGTACA 
CATGCTGAGA ATGATCTATT TGCCCAAAGG AAACGACTGG TGTCCCTGAA GAGTCGCCGA 
GGTGTGGAGC GAGTGGAGGA AAAGGAAACA GTGCATTACT ACCGTGATCC GAAGATTGAG 
GCTGACTTGG AGGTGCTAAA GAAGAGGATA GCAGACGAGA TCTACAAGCG ATCCGAAATG 
CAATCCGAAA TTGAGATTTT CAAGCAAAAA ATTGTCAGCC GTGAGCGAGA TGTTGTAGAA 
GTAAAGCCCA GGTTGGTGAC AAAGGTGGTT ACAGAAATTG AGCGTGACCC CAAATTAGAT 
GTGGAAGCAG CGAGGATTAG GGAGCTCATA CTTAAACTAA AAGAGGAAAT TCGAGTGCTT 
GAGAGTAATT CAGTTAACAT CCGAACGGAG GTAACTGTCC TGGAGAACAA GAAGCCTGTG 
ATAAAGGAAA GGGTCATCCT TAAAGAGGTT GTGAGGCTGG AAAAGGACCC AGAAATGCTG 
AAAACTGTAA TGAGCTTCAA GAGCGAGATA TCGGATGAGA GTATCAGATG CAAGAACCTG 
AATGATGAAA TATTCGTTAT TAGGAGTCAG ATCAATAACT TGGAGAGAAT TATTCCAACC 
ATACAACCCA AAACTATCAT CAAAGAGGTA AAGAGGGTGG AACAAGATGC TGACCTCCTA 
CAAGAGTCCA GAACTCTTCG TGTGACCCTG GAAGAGCTGA TCCAAGAGTA CAGTATTTTG 
ATGAAAGAGA TCTCCAGCCT TCAGGTCCGC TACTCCCAAG TAGAAACCAT CAGACAGAAG 
ATCGAGTTCA ATGAGATCAT TCATGAGATC TACAGGATTG ATCCTGAGAC GGAGAGGGAG 
CTTCGACGGC TGAGAAACGA ACTTATCGAA ATAGGCAAAC GCCGCACTAG CATTGAAAGT 
GAGATTAGTT TAATTATGGT GGATCTGAAG ACTCTGCGTG CCGAGAAACC CAAAGTTGAG 
CTGAAGGAGG TGACTCGAGA GGTTGTGAAA GAAGAGAGGA GCCCTGAGAT TATCAGAGAA 
ATTAAGAGGC TGAATGAGCA GCTTACCATT TTGCGTACCA ACTACGATTC TACTACGAGT 
CGTGTGTTTC GTCTGCGCAA GGACAGGGAT GAGTGGAAGG CTGAAAGATC CAAGGTGGAA 
ACGAAAGTGG TGACCAAAGA GTTGATAAGG TATGAAAATG ACCCTCTTCT GGAGAAAGAA 
GCTGAACGTC TTAGGAAGGA AGTCAGAGAG GAAATTCAAT GCCGCCGAAC AGTGGAAGAG 
AATGTGTTTG ATCTTCAAAA CAAGTACATT GTGCTCGAGA GACAGAAACC AGAGGAAAGA 
GTTGTGGTCC AAGAAATAGT GCGTCTCCAG ACAGATCCGA GTCAGATTCA GACTCATGAG 
AGATTAGTCA GGACTTTGGA AGAGACTATC AAGGTTCGAC GACAGCTAGA ACTGGAGGTG 
CAGCAGATGA GATCTGTAAT CCTGGAACTG GAGAAAAAAC TGGAGCTTGT GGATCGACAG 
AAGAAGGTAA TGGTTGAGAC AGAGTTGAGA CAAATCAAGG CTAGAATCTA TGAACTTGAG 
AACTGCCCAA AACCTGTTGA GGAAAAGATC GTCATTGAGG AGGTTTTGAA AGTTGAGAGA 
GACTCTAAAG TCGACAAACT TATCACTGGG CTTCGCACTG AGATTGACAA TGAGGGTGCC 
AGCATTTCAA GACTGGAGAG AGAGATCCGA AACCTCAGAA TTAGGATTGA GTCCCTGACC 
AAGGAGAAAT CTGTTGAGAG GATCATCCAC AAAGAGGTGA TCAGGGTTGA GAAGGACCAA 
GCAGTGGAGG CAGAGAGAGA GAGCCTTCTG ACCCAAGTCT CTCAAGTGAA GAGTACCAGG 
ACCCTTAAAG ATGAAGAACT GCTGAGATTG AAAAACAAAA TCACCCGAAT TCAGACCACC 
AGGACAAACT TCTCTCAAGA GGAGACCACC TTGATTGCCA ACAGGGATAG GATCAAGAGG 
GAGCAAAACG AACTTCTCCA GGAGCTGAAA AGACTGGAGA CTCAAAAGCA GGAGATCAGC 
ATAACTTTCC AACAACAGAC CAAACTCCTG AGCGAGAGGA ACCAACTCAG CAGACAAAAG 
AGCATCAAGA TGGAGTCAGA GATCCAGCGC CTGGAAAGGG AAATCCTGGA GGTGAAAGAA 
CTGATCAACG TTCGGGAAAA AACTCTCATC GAGCTCCAAA AAGAGGCAAA GTCTGTGCAG 
AATACAGAGA CAAACACCAG AGAAACTAAC GTCTCTACCA AAATCACCAT TCTGGATCCA 
GAGACTGGCA AAGACATGTC TCCATACGAC GCCTATCTTG AGGGCCTGAT TGACCGCAAT 
CAGTACATCC ACCTGCAGGA GCTGGAGTGC AGCTGGGAAG AAATCACAAC CACGGGACCT 
GACGGTGAAG TCACACTCCT GCAAGATCGT AAAAGTGGAA AGCAGTACTC CATCAAGACG 
GCTCTCCAAA ATGGACGACT AACCCAATAT GACCTTCAGC GCTACAGAGA TGGTAAAATG 
ACCATTTCGG AGTTCGCTCT CTTGGTTGCA GGAGAAAAAA AATCAACAGT CTTTCCTCCC 
TCGAGTGTAA CACAAACAAC ATCCCTTCAG ACAAACTCCA GCTACAGCCC CTCAACACTG 
ACACGGCGTA CATCCTACTC CAGCAACGGC AACTTAAACT CTCTGAACAC AAGCGGAGAT 
GAGCTCTACC CTATTTCTGG CATTCTGGAC ATCACCACAG AAAGCCGAAT GTCTGTAAGA 
AGTGCCCTAA CACGCAAACT GATTGATCAA GACACCGCAA TGAGGCTCCT CGAAGCTCAG 
GCTGCTACTG GAGGAATTGT GGACCTCAAT AAGAAAGACA AGCTGTCCGT TCATAAGGCT 
GCAGAGCGTG GATTGATCGA CTCCAGCCAA CTTCACAAAC TCCTCAGTGC CCAAAAGGCA 
TTCACTGGGT TTGAAGACCC TGTGTCTAAA GAGCGCATGT CAGTGGCAGA AGCCGCTCGT 
AGAGGATGGA TCCCAAATGA TAGTGCTATA TGGTTTATGG AGGCACAGCT GTTAACCGGT 
GGCCTTGTTG ACCCAAACCG AGCAGGTCGC ATCGGTCTGC AAAACGCAAT TGATGCAAAA 
TTGATCGACA CATCGATGGC CAAGCAACTC CAAGATGATC TGGCCTTAGT GAAGAACGTT 
GTGGATCCCG TAAAAAAGGA TAAAATCACA TATAAAGAGG CCATGGCTCG ATGCAAGCGG 
GATCACAGCA CGGGACTGCT CCTGTTTCCG GCAGCCTCCA CTGACACCGT AGACGCTCCG 
TCCTATTCCA GCTACAAATT CACCAAGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001922073.6
CDS	165..6197
Translation	MFKKKEYSPAKAGQAQVNDLLSLVTRLQQSADQVEKNILLADKLLEEDLENERRGSPLRQQGPAAENLSQAEMLLKDLFMDVSKVKKLQHPQASIIERDVSNLHDRWSKYCGTYRHLYGQLHELYLPPGVDWSKLMSDKQKLLQDEAYGPTLTDVEKQIAQHNILHKQIEEYHRALEDSGDNSSALQKQYAALLASSAGRRAHLSSLYELMQACSRELQYLSEQQQKIISRDWSDRIIDSAGVRMENERFKNNGLLTHEREVSKLQEEANALIQDKHPGAAAVKSYSADVTREWKQFLSLCLCQDTHLDNVEEYKKYQLNLETFTESLNRIKSTTEPSALRNMSNPEILLQLEAEERSMLRNEQRLADLRELSSSIAPLHLRRSTTGRSISAVALCDWTTDKDSVKRGEKVTLVSSPREQMWEVSSSSGGTKLIPGACFMIPPPDSAAIDKVDSLSRDFSDLKRTRSALQAQAKTPNIEVVQFVKQVAVATVPDSAKASVIDSRLDQIYSDLLKLEKDMQNRIRTPLDRSAPVGDLSARIKDQESFALVLKNLEAEKASAQRDMQPLISQKPLGPTTSSLPLKLSKVNNKIDDISALSDLYHKKATSAMFLEKQIRMVDGVLTDFEDQLCADSGLLDEDNAIRNHSRQLQQLSKDVASRKGDIQQLNGELEATEQTCSSLQKSFQEFCPDIRHQESQVKRLRNRYSNINSQLQQRATLLKEAVSKYQEFQSVNQSLTAFLINMPNNKVQPGESMTQVYAKQNSQMRAVEDIKRKQDDVNRIVKLSEELQGVLNEYEVNSNKYRSALGKPEINSKGLDHLTLAEFVKKKEKDVVNLYSEVSAENNQLLNQLYLAKDLIYKNEEIVNHVVVKQQLQMQSQQREMEEVDGLKQELFEEISRRTHAENDLFAQRKRLVSLKSRRGVERVEEKETVHYYRDPKIEADLEVLKKRIADEIYKRSEMQSEIEIFKQKIVSRERDVVEVKPRLVTKVVTEIERDPKLDVEAARIRELILKLKEEIRVLESNSVNIRTEVTVLENKKPVIKERVILKEVVRLEKDPEMLKTVMSFKSEISDESIRCKNLNDEIFVIRSQINNLERIIPTIQPKTIIKEVKRVEQDADLLQESRTLRVTLEELIQEYSILMKEISSLQVRYSQVETIRQKIEFNEIIHEIYRIDPETERELRRLRNELIEIGKRRTSIESEISLIMVDLKTLRAEKPKVELKEVTREVVKEERSPEIIREIKRLNEQLTILRTNYDSTTSRVFRLRKDRDEWKAERSKVETKVVTKELIRYENDPLLEKEAERLRKEVREEIQCRRTVEENVFDLQNKYIVLERQKPEERVVVQEIVRLQTDPSQIQTHERLVRTLEETIKVRRQLELEVQQMRSVILELEKKLELVDRQKKVMVETELRQIKARIYELENCPKPVEEKIVIEEVLKVERDSKVDKLITGLRTEIDNEGASISRLEREIRNLRIRIESLTKEKSVERIIHKEVIRVEKDQAVEAERESLLTQVSQVKSTRTLKDEELLRLKNKITRIQTTRTNFSQEETTLIANRDRIKREQNELLQELKRLETQKQEISITFQQQTKLLSERNQLSRQKSIKMESEIQRLEREILEVKELINVREKTLIELQKEAKSVQNTETNTRETNVSTKITILDPETGKDMSPYDAYLEGLIDRNQYIHLQELECSWEEITTTGPDGEVTLLQDRKSGKQYSIKTALQNGRLTQYDLQRYRDGKMTISEFALLVAGEKKSTVFPPSSVTQTTSLQTNSSYSPSTLTRRTSYSSNGNLNSLNTSGDELYPISGILDITTESRMSVRSALTRKLIDQDTAMRLLEAQAATGGIVDLNKKDKLSVHKAAERGLIDSSQLHKLLSAQKAFTGFEDPVSKERMSVAEAARRGWIPNDSAIWFMEAQLLTGGLVDPNRAGRIGLQNAIDAKLIDTSMAKQLQDDLALVKNVVDPVKKDKITYKEAMARCKRDHSTGLLLFPAASTDTVDAPSYSSYKFTKK

Target ORF information:

RefSeq Version	XM_001922038.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio envoplakin b (evplb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922038.6

ORF Insert Sequence:

ATGTTCAAGA AGAAGGAATA CAGCCCTGCT AAAGCCGGAC AGGCGCAGGT GAACGACCTG 
CTGTCTCTGG TCACACGCTT ACAGCAGAGC GCAGATCAGG TGGAGAAGAA CATCCTGCTG 
GCCGACAAAC TGCTGGAGGA GGATCTGGAG AATGAGCGCA GGGGCTCTCC TCTCAGACAG 
CAGGGTCCGG CCGCTGAGAA TCTGTCTCAG GCTGAGATGC TGCTGAAGGA TCTGTTCATG 
GACGTCAGCA AAGTCAAGAA GCTCCAGCAC CCGCAGGCCA GCATCATCGA GCGCGATGTG 
AGTAACCTGC ACGATCGTTG GTCCAAATAC TGCGGCACAT ACAGGCATCT GTACGGGCAG 
CTTCACGAGC TCTACCTGCC GCCCGGAGTG GACTGGAGCA AACTGATGAG TGACAAACAG 
AAACTCCTGC AGGACGAGGC TTACGGCCCG ACTCTGACTG ATGTGGAGAA GCAGATCGCA 
CAACACAACA TCCTGCACAA GCAGATAGAG GAGTACCACA GAGCCCTGGA GGACAGCGGA 
GATAACTCGT CTGCTCTTCA GAAGCAGTAT GCGGCTCTGC TGGCGTCATC AGCAGGTCGT 
CGTGCGCATC TGTCGTCGCT GTATGAGCTG ATGCAGGCCT GCAGTAGAGA GCTGCAGTAT 
CTCAGTGAGC AGCAGCAGAA GATCATCAGC AGAGACTGGA GCGACCGCAT CATCGACAGC 
GCTGGAGTGC GCATGGAGAA CGAGAGGTTT AAGAACAACG GTCTGCTCAC ACACGAAAGA 
GAAGTGAGCA AACTACAGGA GGAGGCCAAC GCTCTCATTC AGGACAAACA CCCTGGCGCA 
GCTGCTGTCA AGTCGTACAG TGCAGACGTC ACAAGAGAAT GGAAACAATT CCTCAGTTTG 
TGTCTGTGTC AGGACACACA CCTGGACAAC GTGGAGGAGT ACAAGAAATA TCAGCTGAAT 
CTGGAGACGT TCACTGAGTC GCTGAATAGA ATCAAATCCA CCACTGAACC ATCAGCTCTG 
AGAAACATGA GCAATCCTGA GATCCTGCTG CAGCTCGAGG CGGAGGAGCG GTCGATGCTG 
AGGAACGAGC AGCGTCTGGC GGACCTGAGA GAGTTGAGCT CCTCCATCGC TCCTCTACAT 
CTGCGCAGAT CCACCACCGG CAGATCCATC AGCGCCGTCG CTCTGTGTGA CTGGACCACC 
GACAAGGACT CGGTAAAGCG CGGTGAGAAG GTAACACTGG TCTCCAGCCC CAGAGAGCAG 
ATGTGGGAGG TCAGTTCCAG CAGCGGAGGT ACCAAACTGA TCCCTGGAGC CTGTTTCATG 
ATCCCTCCGC CTGATTCTGC TGCCATCGAC AAGGTGGACA GCCTGAGCAG AGATTTCTCG 
GATCTGAAGA GGACCAGATC GGCTCTACAG GCTCAGGCCA AAACACCCAA CATTGAAGTG 
GTGCAGTTCG TCAAACAAGT CGCTGTGGCC ACTGTTCCTG ACAGTGCAAA GGCCAGTGTC 
ATCGACAGCC GTCTGGACCA GATCTACTCA GACCTGCTGA AGCTGGAGAA AGACATGCAG 
AACCGCATCA GAACTCCACT GGACCGCTCC GCGCCTGTCG GAGACCTGTC TGCCAGAATC 
AAAGACCAGG AGAGTTTTGC ATTGGTTCTG AAGAATCTGG AGGCAGAGAA AGCTTCAGCT 
CAGAGGGACA TGCAACCCCT GATCTCCCAG AAGCCCCTGG GGCCCACCAC ATCCTCGCTG 
CCTCTGAAAC TGAGCAAAGT CAACAACAAG ATTGATGACA TCAGCGCACT CTCCGACCTA 
TACCACAAGA AGGCCACGTC TGCCATGTTC CTGGAGAAGC AGATCAGGAT GGTGGACGGT 
GTGCTGACGG ACTTCGAGGA TCAGCTGTGT GCAGATTCGG GGCTGTTAGA CGAAGACAAT 
GCCATTCGCA ACCACTCCAG ACAACTGCAG CAATTGAGTA AGGACGTTGC ATCCAGGAAG 
GGAGACATTC AGCAGCTGAA CGGTGAGCTG GAGGCCACCG AGCAGACGTG CAGCTCCCTG 
CAGAAGAGTT TTCAGGAGTT CTGCCCGGAT ATCCGCCATC AGGAGAGCCA AGTCAAGCGC 
TTGAGGAACC GATACTCCAA CATCAACAGC CAGCTGCAGC AGAGGGCAAC TCTGTTGAAA 
GAAGCTGTCA GTAAGTACCA GGAGTTTCAG AGCGTCAACC AATCGCTCAC TGCTTTCCTC 
ATCAACATGC CCAACAACAA GGTGCAGCCT GGTGAAAGTA TGACGCAAGT CTATGCCAAG 
CAAAACTCGC AGATGAGAGC AGTTGAAGAC ATCAAGAGGA AGCAGGACGA TGTGAACAGG 
ATCGTGAAAC TTTCCGAGGA GCTGCAGGGC GTTCTCAATG AGTATGAGGT CAATTCTAAC 
AAATACCGCA GTGCATTGGG AAAGCCGGAA ATTAATTCAA AAGGACTTGA TCACTTAACC 
CTGGCTGAAT TTGTGAAGAA GAAGGAAAAG GATGTTGTGA ATCTATACTC TGAAGTATCT 
GCTGAGAATA ACCAGCTTCT GAATCAACTG TACTTGGCCA AAGATCTCAT CTACAAGAAT 
GAAGAAATCG TGAACCATGT GGTCGTAAAG CAGCAACTGC AGATGCAAAG TCAGCAGAGA 
GAAATGGAGG AAGTTGACGG CTTGAAGCAA GAGCTTTTTG AGGAAATATC TCGTCGTACA 
CATGCTGAGA ATGATCTATT TGCCCAAAGG AAACGACTGG TGTCCCTGAA GAGTCGCCGA 
GGTGTGGAGC GAGTGGAGGA AAAGGAAACA GTGCATTACT ACCGTGATCC GAAGATTGAG 
GCTGACTTGG AGGTGCTAAA GAAGAGGATA GCAGACGAGA TCTACAAGCG ATCCGAAATG 
CAATCCGAAA TTGAGATTTT CAAGCAAAAA ATTGTCAGCC GTGAGCGAGA TGTTGTAGAA 
GTAAAGCCCA GGTTGGTGAC AAAGGTGGTT ACAGAAATTG AGCGTGACCC CAAATTAGAT 
GTGGAAGCAG CGAGGATTAG GGAGCTCATA CTTAAACTAA AAGAGGAAAT TCGAGTGCTT 
GAGAGTAATT CAGTTAACAT CCGAACGGAG GTAACTGTCC TGGAGAACAA GAAGCCTGTG 
ATAAAGGAAA GGGTCATCCT TAAAGAGGTT GTGAGGCTGG AAAAGGACCC AGAAATGCTG 
AAAACTGTAA TGAGCTTCAA GAGCGAGATA TCGGATGAGA GTATCAGATG CAAGAACCTG 
AATGATGAAA TATTCGTTAT TAGGAGTCAG ATCAATAACT TGGAGAGAAT TATTCCAACC 
ATACAACCCA AAACTATCAT CAAAGAGGTA AAGAGGGTGG AACAAGATGC TGACCTCCTA 
CAAGAGTCCA GAACTCTTCG TGTGACCCTG GAAGAGCTGA TCCAAGAGTA CAGTATTTTG 
ATGAAAGAGA TCTCCAGCCT TCAGGTCCGC TACTCCCAAG TAGAAACCAT CAGACAGAAG 
ATCGAGTTCA ATGAGATCAT TCATGAGATC TACAGGATTG ATCCTGAGAC GGAGAGGGAG 
CTTCGACGGC TGAGAAACGA ACTTATCGAA ATAGGCAAAC GCCGCACTAG CATTGAAAGT 
GAGATTAGTT TAATTATGGT GGATCTGAAG ACTCTGCGTG CCGAGAAACC CAAAGTTGAG 
CTGAAGGAGG TGACTCGAGA GGTTGTGAAA GAAGAGAGGA GCCCTGAGAT TATCAGAGAA 
ATTAAGAGGC TGAATGAGCA GCTTACCATT TTGCGTACCA ACTACGATTC TACTACGAGT 
CGTGTGTTTC GTCTGCGCAA GGACAGGGAT GAGTGGAAGG CTGAAAGATC CAAGGTGGAA 
ACGAAAGTGG TGACCAAAGA GTTGATAAGG TATGAAAATG ACCCTCTTCT GGAGAAAGAA 
GCTGAACGTC TTAGGAAGGA AGTCAGAGAG GAAATTCAAT GCCGCCGAAC AGTGGAAGAG 
AATGTGTTTG ATCTTCAAAA CAAGTACATT GTGCTCGAGA GACAGAAACC AGAGGAAAGA 
GTTGTGGTCC AAGAAATAGT GCGTCTCCAG ACAGATCCGA GTCAGATTCA GACTCATGAG 
AGATTAGTCA GGACTTTGGA AGAGACTATC AAGGTTCGAC GACAGCTAGA ACTGGAGGTG 
CAGCAGATGA GATCTGTAAT CCTGGAACTG GAGAAAAAAC TGGAGCTTGT GGATCGACAG 
AAGAAGGTAA TGGTTGAGAC AGAGTTGAGA CAAATCAAGG CTAGAATCTA TGAACTTGAG 
AACTGCCCAA AACCTGTTGA GGAAAAGATC GTCATTGAGG AGGTTTTGAA AGTTGAGAGA 
GACTCTAAAG TCGACAAACT TATCACTGGG CTTCGCACTG AGATTGACAA TGAGGGTGCC 
AGCATTTCAA GACTGGAGAG AGAGATCCGA AACCTCAGAA TTAGGATTGA GTCCCTGACC 
AAGGAGAAAT CTGTTGAGAG GATCATCCAC AAAGAGGTGA TCAGGGTTGA GAAGGACCAA 
GCAGTGGAGG CAGAGAGAGA GAGCCTTCTG ACCCAAGTCT CTCAAGTGAA GAGTACCAGG 
ACCCTTAAAG ATGAAGAACT GCTGAGATTG AAAAACAAAA TCACCCGAAT TCAGACCACC 
AGGACAAACT TCTCTCAAGA GGAGACCACC TTGATTGCCA ACAGGGATAG GATCAAGAGG 
GAGCAAAACG AACTTCTCCA GGAGCTGAAA AGACTGGAGA CTCAAAAGCA GGAGATCAGC 
ATAACTTTCC AACAACAGAC CAAACTCCTG AGCGAGAGGA ACCAACTCAG CAGACAAAAG 
AGCATCAAGA TGGAGTCAGA GATCCAGCGC CTGGAAAGGG AAATCCTGGA GGTGAAAGAA 
CTGATCAACG TTCGGGAAAA AACTCTCATC GAGCTCCAAA AAGAGGCAAA GTCTGTGCAG 
AATACAGAGA CAAACACCAG AGAAACTAAC GTCTCTACCA AAATCACCAT TCTGGATCCA 
GAGACTGGCA AAGACATGTC TCCATACGAC GCCTATCTTG AGGGCCTGAT TGACCGCAAT 
CAGTACATCC ACCTGCAGGA GCTGGAGTGC AGCTGGGAAG AAATCACAAC CACGGGACCT 
GACGGTGAAG TCACACTCCT GCAAGATCGT AAAAGTGGAA AGCAGTACTC CATCAAGACG 
GCTCTCCAAA ATGGACGACT AACCCAATAT GACCTTCAGC GCTACAGAGA TGGTAAAATG 
ACCATTTCGG AGTTCGCTCT CTTGGTTGCA GGAGAAAAAA AATCAACAGT CTTTCCTCCC 
TCGAGTGTAA CACAAACAAC ATCCCTTCAG ACAAACTCCA GCTACAGCCC CTCAACACTG 
ACACGGCGTA CATCCTACTC CAGCAACGGC AACTTAAACT CTCTGAACAC AAGCGGAGAT 
GAGCTCTACC CTATTTCTGG CATTCTGGAC ATCACCACAG AAAGCCGAAT GTCTGTAAGA 
AGTGCCCTAA CACGCAAACT GATTGATCAA GACACCGCAA TGAGGCTCCT CGAAGCTCAG 
GCTGCTACTG GAGGAATTGT GGACCTCAAT AAGAAAGACA AGCTGTCCGT TCATAAGGCT 
GCAGAGCGTG GATTGATCGA CTCCAGCCAA CTTCACAAAC TCCTCAGTGC CCAAAAGGCA 
TTCACTGGGT TTGAAGACCC TGTGTCTAAA GAGCGCATGT CAGTGGCAGA AGCCGCTCGT 
AGAGGATGGA TCCCAAATGA TAGTGCTATA TGGTTTATGG AGGCACAGCT GTTAACCGGT 
GGCCTTGTTG ACCCAAACCG AGCAGGTCGC ATCGGTCTGC AAAACGCAAT TGATGCAAAA 
TTGATCGACA CATCGATGGC CAAGCAACTC CAAGATGATC TGGCCTTAGT GAAGAACGTT 
GTGGATCCCG TAAAAAAGGA TAAAATCACA TATAAAGAGG CCATGGCTCG ATGCAAGCGG 
GATCACAGCA CGGGACTGCT CCTGTTTCCG GCAGCCTCCA CTGACACCGT AGACGCTCCG 
TCCTATTCCA GCTACAAATT CACCAAGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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EVPLB cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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