pnp5a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pnp5a
Entrez Gene ID	791647
Full Name	purine nucleoside phosphorylase 5a
Synonyms	fc27g06,np,wu:fc27g06,wu:fd51h11,zgc:77008
General protein information	Preferred Names purine nucleoside phosphorylase 5a Names purine nucleoside phosphorylase 5a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_213311.2	NP_998476.1	purine nucleoside phosphorylase 5a

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pnp5b	NP_001004628.1
Danio rerio (zebrafish)	pnp5a	NP_998476.1
Danio rerio (zebrafish)	pnp6	NP_991218.2

Gene Ontology
Bibliography

Related articles in PubMed

Kimura T, Takehana Y, Naruse K
G3 (Bethesda, Md.)7(4)1357-1363(2017 04)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Different expression profiles of Interleukin 11 (IL-11), Intelectin (ITLN) and Purine nucleoside phosphorylase 5a (PNP 5a) in crucian carp (Carassius auratus gibelio) in response to Cyprinid herpesvirus 2 and Aeromonas hydrophila.
Podok P, Xu L, Xu D, Lu L
Fish & shellfish immunology38(1)65-73(2014 May)

Identification and expression of liver-specific genes after LPS challenge in amphioxus: the hepatic cecum as liver-like organ and "pre-hepatic" acute phase response.
Wang Y, Zhang S
Functional & integrative genomics11(1)111-8(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pnp5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pnp5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa34109	NM_213311.2 Latest version!	Danio rerio purine nucleoside phosphorylase 5a (pnp5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pnp5a ( NM_213311.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998476.1 Danio rerio purine nucleoside phosphorylase 5a (pnp5a), mRNA.

Price & Availability↑

CloneID	ODa34109
Clone ID Related Accession (Same CDS sequence)	NM_213311.2
Accession Version	NM_213311.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	purine nucleoside phosphorylase 5a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU464117.4, EB931190.1 and EB919906.1. On Dec 3, 2011 this sequence version replaced NM_213311.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01008601.1, BC066610.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTTTCCCG AGAGCAACAC TGGGTACAGT TTCGAGGATT GCCAGGCCAC CGCTGATTGG 
CTCCTGTCCC AGACTGCAGT GCGCCCTCTG GTGGGCATCG TGTGTGGTTC GGGTTTGGGT 
GGATTGGCTG ATGCTCTGAA AGACCAGGTG GCCTTCAACT ACCGGGACAT TCCTAACTTT 
CCCCAGAGTA CAGTTCACGG TCATGCGGGT CGGCTGGTGT TTGGCACTTT AAAGGGCAGG 
CCGTGTGTCT GCATGCAGGG ACGCTTTCAT CTCTATGAAG GATATCCCAT CCAGAAGATT 
ACGATGCCGA TGCGGATTTT CAAGATGCTG GGTGTGGAGA CGGTGATTCT TACCAACGCT 
GCTGGCGGTC TGAACCAGGA CTATAAAGTA GGAGACATCA TGATCATCAA AGACCACCTC 
AACATGCCCG GATTCGCTGG AAACAACCCT CTGGCGGGAC ACAACGACGA AAGGTTTGGA 
GTGCGTTTCC CCTGCATGTC TGACGCGTAT GATCGAGAGC TGCAGCAGAT GGCTCTGGAT 
GTGGGATCAG AGCTGGGCTT CAGTGATTTC CTGCGGGAGG GGGTTTACTG TGTTCTTGGA 
GGACCGTCAT TCGAGACCAT CGCTGAGTGC CGCATGCTGC ACAAACTGGG TGCTGACGCT 
GTCGGCATGA GCACAGTTCA TGAGGTGATC GTAGCCCGTC ACTGCGGGAT GCGCGTCTTC 
GCTCTGTCTT TGATAACCAA TAAAGCAGTT ATGGATTACG ACAGCGAGGA AAAAGCCAAC 
CATGAGGAGG TCCTGCAGAC GGGCAAACAG CGAGCGGAGC AGCTGGAGAG ACTGGTGTCT 
ACTATTATTA CCCGGCTGGA GCACAACAAC AACTACGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998476.1
CDS	229..1110
Translation	MFPESNTGYSFEDCQATADWLLSQTAVRPLVGIVCGSGLGGLADALKDQVAFNYRDIPNFPQSTVHGHAGRLVFGTLKGRPCVCMQGRFHLYEGYPIQKITMPMRIFKMLGVETVILTNAAGGLNQDYKVGDIMIIKDHLNMPGFAGNNPLAGHNDERFGVRFPCMSDAYDRELQQMALDVGSELGFSDFLREGVYCVLGGPSFETIAECRMLHKLGADAVGMSTVHEVIVARHCGMRVFALSLITNKAVMDYDSEEKANHEEVLQTGKQRAEQLERLVSTIITRLEHNNNYA

Target ORF information:

RefSeq Version	NM_213311.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio purine nucleoside phosphorylase 5a (pnp5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213311.2

ORF Insert Sequence:

ATGTTTCCCG AGAGCAACAC TGGGTACAGT TTCGAGGATT GCCAGGCCAC CGCTGATTGG 
CTCCTGTCCC AGACTGCAGT GCGCCCTCTG GTGGGCATCG TGTGTGGTTC GGGTTTGGGT 
GGATTGGCTG ATGCTCTGAA AGACCAGGTG GCCTTCAACT ACCGGGACAT TCCTAACTTT 
CCCCAGAGTA CAGTTCACGG TCATGCGGGT CGGCTGGTGT TTGGCACTTT AAAGGGCAGG 
CCGTGTGTCT GCATGCAGGG ACGCTTTCAT CTCTATGAAG GATATCCCAT CCAGAAGATT 
ACGATGCCGA TGCGGATTTT CAAGATGCTG GGTGTGGAGA CGGTGATTCT TACCAACGCT 
GCTGGCGGTC TGAACCAGGA CTATAAAGTA GGAGACATCA TGATCATCAA AGACCACCTC 
AACATGCCCG GATTCGCTGG AAACAACCCT CTGGCGGGAC ACAACGACGA AAGGTTTGGA 
GTGCGTTTCC CCTGCATGTC TGACGCGTAT GATCGAGAGC TGCAGCAGAT GGCTCTGGAT 
GTGGGATCAG AGCTGGGCTT CAGTGATTTC CTGCGGGAGG GGGTTTACTG TGTTCTTGGA 
GGACCGTCAT TCGAGACCAT CGCTGAGTGC CGCATGCTGC ACAAACTGGG TGCTGACGCT 
GTCGGCATGA GCACAGTTCA TGAGGTGATC GTAGCCCGTC ACTGCGGGAT GCGCGTCTTC 
GCTCTGTCTT TGATAACCAA TAAAGCAGTT ATGGATTACG ACAGCGAGGA AAAAGCCAAC 
CATGAGGAGG TCCTGCAGAC GGGCAAACAG CGAGCGGAGC AGCTGGAGAG ACTGGTGTCT 
ACTATTATTA CCCGGCTGGA GCACAACAAC AACTACGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

G3 (Bethesda, Md.)7(4)1357-1363(2017 04)
Kimura T,Takehana Y,Naruse K

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Different expression profiles of Interleukin 11 (IL-11), Intelectin (ITLN) and Purine nucleoside phosphorylase 5a (PNP 5a) in crucian carp (Carassius auratus gibelio) in response to Cyprinid herpesvirus 2 and Aeromonas hydrophila.
Fish & shellfish immunology38(1)65-73(2014 May)
Podok P,Xu L,Xu D,Lu L

Identification and expression of liver-specific genes after LPS challenge in amphioxus: the hepatic cecum as liver-like organ and "pre-hepatic" acute phase response.
Functional & integrative genomics11(1)111-8(2011 Mar)
Wang Y,Zhang S

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pnp5a cDNA ORF clone, Danio rerio(zebrafish)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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