OCRL cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	OCRL
Entrez Gene ID	777716
Full Name	oculocerebrorenal syndrome of Lowe
Synonyms	wu:fi09g03,zgc:152864
General protein information	Preferred Names oculocerebrorenal syndrome of Lowe Names inositol polyphosphate 5-phosphatase OCRL-1 OCRL1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009291139.3	XP_009289414.1	inositol polyphosphate 5-phosphatase OCRL-1 isoform X1
NM_001077764.2	NP_001071232.2	inositol polyphosphate 5-phosphatase OCRL-1
XM_009291139.2	XP_009289414.1	inositol polyphosphate 5-phosphatase OCRL-1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	OCRL	NP_000267.2
Pan troglodytes (chimpanzee)	OCRL	XP_003317719.2
Rattus norvegicus (Norway rat)	Ocrl	NP_001101726.2
Canis lupus familiaris (dog)	OCRL	XP_852376.2
Mus musculus (house mouse)	Ocrl	NP_796189.2
Xenopus tropicalis (tropical clawed frog)	ocrl	XP_004919252.1
Gallus gallus (chicken)	OCRL	XP_004940632.1
Danio rerio (zebrafish)	LOC100329312	XP_003199958.1
Macaca mulatta (Rhesus monkey)	OCRL	XP_002806438.1
Danio rerio (zebrafish)	ocrl	NP_001071232.1
Drosophila melanogaster (fruit fly)	Ocrl	NP_569962.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010556	XP_558090.3
Bos taurus (cattle)	OCRL	NP_001095661.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Apela Regulates Fluid Homeostasis by Binding to the APJ Receptor to Activate Gi Signaling.
Deng C, Chen H, Yang N, Feng Y, Hsueh AJ
The Journal of biological chemistry290(30)18261-8(2015 Jul)

The Lowe syndrome protein OCRL1 is required for endocytosis in the zebrafish pronephric tubule.
Oltrabella F, Pietka G, Ramirez IB, Mironov A, Starborg T, Drummond IA, Hinchliffe KA, Lowe M
PLoS genetics11(4)e1005058(2015 Apr)

Compensatory Role of Inositol 5-Phosphatase INPP5B to OCRL in Primary Cilia Formation in Oculocerebrorenal Syndrome of Lowe.
Luo N, Kumar A, Conwell M, Weinreb RN, Anderson R, Sun Y
PloS one8(6)e66727(2013)

Lowe syndrome: Between primary cilia assembly and Rac1-mediated membrane remodeling.
Madhivanan K, Mukherjee D, Aguilar RC
Communicative & integrative biology5(6)641-4(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following OCRL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OCRL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa53342	NM_001077764.2 Latest version!	Danio rerio OCRL inositol polyphosphate-5-phosphatase (ocrl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa46586	XM_009291139.3 Latest version!	Danio rerio oculocerebrorenal syndrome of Lowe (ocrl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
ODa46586	XM_009291139.2	Danio rerio oculocerebrorenal syndrome of Lowe (ocrl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OCRL ( NM_001077764.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001071232.2 Danio rerio OCRL inositol polyphosphate-5-phosphatase (ocrl), mRNA.

Price & Availability↑

CloneID	ODa53342
Clone ID Related Accession (Same CDS sequence)	NM_001077764.2
Accession Version	NM_001077764.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554566400000
Organism	Danio rerio(zebrafish)
Product	inositol polyphosphate 5-phosphatase OCRL-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FP016208.12. On May 26, 2016 this sequence version replaced NM_001077764.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01017112.1, BC125826.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGATGGAAC CTTCATTTGG GATTTCCGAA AGCCAGTGTA TAGCCACTGT TAGAGGACAT 
GAAATCAGAA GCGGACAGAA AGAACCAAGA CTCCTGTCCT TGTTAGAGTG TTCTGGGCAG 
TTCAAGTTGG ATATCCTTTC AAATCCTGAT GCAATTGTAT TAAGCCCGGT GTCCCAGCTG 
TCCATCCCCA TCAATAACCA TTTTCGGATC ATTCGAGAGG CAGAAGAAGC TCTTCTCATT 
GACATTGACT CTATTAGCAA GACAGTACGA ATACGTCTGC GAGGTGAACG AACACCGGAG 
CTTCTGCTTG AGCTTCAGGA TGACGGGCAA ACGCAGTCTT TTCTTGAGCA TGTGACGAAG 
GCCAAACAGC AAGCTGAGAG GAGTTCACTG CCCAGGAAGA TGGAGCCAGT TGTACCTGCT 
CCAGTTAAAG ACTGTGTTCC AGTTCCACCT CAGAGAGGAA CCTCCAAATC CTTCTCAAAC 
AACATCAACA ACAGCACTTT AGCAAATCCC TCAAACACAG ATTCCACCCG CTCGCTTGCC 
ACAGATTTTG GCTTTGAAGA AAACTTTAAC CACGTTTTAA AAGTGGAAGA GAAGAAGAAC 
AAGCAGAATC GTTTAGTTGC ACAACGAGAG CCACCACCTC CACCAATTCC CCCTCTTCCT 
CCTAGGAAAG GCACAAATAC ATCATTGACA GGCCCAGCTA CCAAAGAAAG GCCACCATCT 
GTACAAAATG CCACACCTGT CAGAAATGAA TTTCAGTCTG TAGACCGATC TGTTTCCTGG 
TCTGAAAGCC CGTCCAACTC CACCATAAGA CAGGCCATGA TGAGCAGCCA TGCAGGGTCA 
AGAGAAGGTT TGCTCAAGTA TCGCCTCAGC AAGAAAGAGA AAGAATATGT GGACATTAAG 
AACTTTAAGT TCTTCGTAGG CACGTGGAAT GTAAATGGTC AGTCCCCTGA CAGCAGTCTT 
GGGCCCTGGC TCAGTTCTGA CCCAGACCCT CCTGACGTCT ATGCACTAGG ATTTCAGGAG 
CTGGACCTGA GCACAGAGGC GTTTTTCTAT ATGGACTCAT CCAAAGAACA GTTATGGGTT 
GATGCAGTAG AGAGAGGCCT CCATCAAAAA GCCAGATACA TTCAGGTTCG TATTATCCGC 
CTAGTGGGCA TGATGTTGGT GGTGTATATC AAAAAGGAGC ACAAAGATCA AATTCGAGAG 
ATCGCTTCTG AGTCTGTGGG AACTGGCCTC ATGAATAAAA TGGGGAACAA GGGAGGAGTG 
GCAGTGCGCT TTGTGTTTCA CAATACCAGC TTCTGCTTCG TAAACTCTCA CTTGGCAGCT 
CATGTGGATG ACTTTGAGCG GCGAAACCAG GACTACAAAG ACATCTGTGC ACGCATGAGT 
TTCCACCTGC TGGAATACCC GCCTGTCAGT ATAGTGAAAC ATGATGTGGT GATTTGGCTT 
GGAGATCTCA ACTACAGGCT ATGCTTGCCT GATGCTGGGG AGGTAAAAAG GCTTATTGCT 
GAAAAAGAGC TACGGAGACT ACAGGAATAT GACCAGCTGA ACCTCCAGAG AAAGACTAAA 
CGTGTCTTCA CCGATTTCAT GGAAGGGGAG ATCAATTTCA TCCCAACTTA CAAATATGAC 
GCTAAATCAG ATCGGTGGGA CTCAAGTGGG AAGTGTCGGA TTCCTGCTTG GTGCGACCGT 
ATCCTATGGA GGGGAAGCAA CGTGAAGCAG CTTCACTACC GCAGTCACAT GGAGCTGAAG 
ACCAGTGACC ACAAACCTGT CAGCTCTGTC TTTAGTATCG GGGTGAAGAT GGTGATTGAG 
CAACGCTACA AAAAGATTTT CGAAGAGATT GTGCGGGACA TGGACAGGAT GGAAAATGAC 
TTTCTGCCCT CTTTGTCCCT AACCCGACGG GAGTTCACCT TTGAGAATGT GAAGTTCCGT 
CAGCTGCAGC GTCAGAGTTT TCTTATCACT AATGATGGAC AAGTTGCTTG CACCTTCGCC 
TTTATTCCCA AACTCAATGA CTCCCAATAC TGTAAGCCCT GGTTACGTGC TGAGCCCTGT 
GAAGGGGTAC TAGATCCTAA TGAGAAAATC GAGATCTTCT TGGAGGTGTA TGTCAGTAAA 
GACTCAGTGA CGCTTTTAAA CTCTGGTGAA GACAACATTG AGGACATCCT GGTGCTTCAT 
CTGGACAGAG GGAAAGACTA CTTCATCACT GTCTCTGGAA ACTACCTGCC CAGTTGTTTC 
GGCACATCTC TGGAGACTCT GTGCCGCATG AAGAAACCTA TCCGTGAGAT CCCCATCACC 
AAACTCATCG ACCTGGAGAA AGCCCGGATG ACATTCTTGT TGGACAGTGC TGGAACAGAA 
GATAAACCTC TAAAGATTCC CAAAGAGGTT TGGTTGCTTG TTAATCACCT CTACACCAAG 
GCCTGCCAGC AGGAGGACCT TTTCCAGACC CCTGGCCTGC AAGACGAGCT ACAAAGTATT 
ATTGACTGCT TGGACACCAG CATTCCTGAT TCCATCCCTG GCTGTAATCA CTCGGTTGCT 
GAAGCTCTAT TGATCTTCTT GGAGGCTCTT CCTGAACCCG TGTTGTGTTA TGAACTCTTT 
CAGCGCTGTC TCGAATGCTC TCATGACAGC CGCCTCTGCA AGCAGCTAAT ATCCCAGCTC 
CCACGAGCCC ATCGCAATGT GTTCCGCTAC CTGATGGCCT TCTTGAGAGA ATTACTCAAA 
CACTCTATTG ACAACAACCT AAACGCCAAC CTCCTAGCTA CTCTCTTCGC AAGCCTCCTC 
ATCCGACCGC CTCCCAACCT TGCTGGCAAA CAGACGCAAC ATGATCGCCA GAAAGCCAAC 
GACTTCATCC TGGGTTTTCT GATGGCAGGC GACGAAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071232.2
CDS	50..2911
Translation	MMEPSFGISESQCIATVRGHEIRSGQKEPRLLSLLECSGQFKLDILSNPDAIVLSPVSQLSIPINNHFRIIREAEEALLIDIDSISKTVRIRLRGERTPELLLELQDDGQTQSFLEHVTKAKQQAERSSLPRKMEPVVPAPVKDCVPVPPQRGTSKSFSNNINNSTLANPSNTDSTRSLATDFGFEENFNHVLKVEEKKNKQNRLVAQREPPPPPIPPLPPRKGTNTSLTGPATKERPPSVQNATPVRNEFQSVDRSVSWSESPSNSTIRQAMMSSHAGSREGLLKYRLSKKEKEYVDIKNFKFFVGTWNVNGQSPDSSLGPWLSSDPDPPDVYALGFQELDLSTEAFFYMDSSKEQLWVDAVERGLHQKARYIQVRIIRLVGMMLVVYIKKEHKDQIREIASESVGTGLMNKMGNKGGVAVRFVFHNTSFCFVNSHLAAHVDDFERRNQDYKDICARMSFHLLEYPPVSIVKHDVVIWLGDLNYRLCLPDAGEVKRLIAEKELRRLQEYDQLNLQRKTKRVFTDFMEGEINFIPTYKYDAKSDRWDSSGKCRIPAWCDRILWRGSNVKQLHYRSHMELKTSDHKPVSSVFSIGVKMVIEQRYKKIFEEIVRDMDRMENDFLPSLSLTRREFTFENVKFRQLQRQSFLITNDGQVACTFAFIPKLNDSQYCKPWLRAEPCEGVLDPNEKIEIFLEVYVSKDSVTLLNSGEDNIEDILVLHLDRGKDYFITVSGNYLPSCFGTSLETLCRMKKPIREIPITKLIDLEKARMTFLLDSAGTEDKPLKIPKEVWLLVNHLYTKACQQEDLFQTPGLQDELQSIIDCLDTSIPDSIPGCNHSVAEALLIFLEALPEPVLCYELFQRCLECSHDSRLCKQLISQLPRAHRNVFRYLMAFLRELLKHSIDNNLNANLLATLFASLLIRPPPNLAGKQTQHDRQKANDFILGFLMAGDED

Target ORF information:

RefSeq Version	NM_001077764.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio OCRL inositol polyphosphate-5-phosphatase (ocrl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077764.2

ORF Insert Sequence:

ATGATGGAAC CTTCATTTGG GATTTCCGAA AGCCAGTGTA TAGCCACTGT TAGAGGACAT 
GAAATCAGAA GCGGACAGAA AGAACCAAGA CTCCTGTCCT TGTTAGAGTG TTCTGGGCAG 
TTCAAGTTGG ATATCCTTTC AAATCCTGAT GCAATTGTAT TAAGCCCGGT GTCCCAGCTG 
TCCATCCCCA TCAATAACCA TTTTCGGATC ATTCGAGAGG CAGAAGAAGC TCTTCTCATT 
GACATTGACT CTATTAGCAA GACAGTACGA ATACGTCTGC GAGGTGAACG AACACCGGAG 
CTTCTGCTTG AGCTTCAGGA TGACGGGCAA ACGCAGTCTT TTCTTGAGCA TGTGACGAAG 
GCCAAACAGC AAGCTGAGAG GAGTTCACTG CCCAGGAAGA TGGAGCCAGT TGTACCTGCT 
CCAGTTAAAG ACTGTGTTCC AGTTCCACCT CAGAGAGGAA CCTCCAAATC CTTCTCAAAC 
AACATCAACA ACAGCACTTT AGCAAATCCC TCAAACACAG ATTCCACCCG CTCGCTTGCC 
ACAGATTTTG GCTTTGAAGA AAACTTTAAC CACGTTTTAA AAGTGGAAGA GAAGAAGAAC 
AAGCAGAATC GTTTAGTTGC ACAACGAGAG CCACCACCTC CACCAATTCC CCCTCTTCCT 
CCTAGGAAAG GCACAAATAC ATCATTGACA GGCCCAGCTA CCAAAGAAAG GCCACCATCT 
GTACAAAATG CCACACCTGT CAGAAATGAA TTTCAGTCTG TAGACCGATC TGTTTCCTGG 
TCTGAAAGCC CGTCCAACTC CACCATAAGA CAGGCCATGA TGAGCAGCCA TGCAGGGTCA 
AGAGAAGGTT TGCTCAAGTA TCGCCTCAGC AAGAAAGAGA AAGAATATGT GGACATTAAG 
AACTTTAAGT TCTTCGTAGG CACGTGGAAT GTAAATGGTC AGTCCCCTGA CAGCAGTCTT 
GGGCCCTGGC TCAGTTCTGA CCCAGACCCT CCTGACGTCT ATGCACTAGG ATTTCAGGAG 
CTGGACCTGA GCACAGAGGC GTTTTTCTAT ATGGACTCAT CCAAAGAACA GTTATGGGTT 
GATGCAGTAG AGAGAGGCCT CCATCAAAAA GCCAGATACA TTCAGGTTCG TATTATCCGC 
CTAGTGGGCA TGATGTTGGT GGTGTATATC AAAAAGGAGC ACAAAGATCA AATTCGAGAG 
ATCGCTTCTG AGTCTGTGGG AACTGGCCTC ATGAATAAAA TGGGGAACAA GGGAGGAGTG 
GCAGTGCGCT TTGTGTTTCA CAATACCAGC TTCTGCTTCG TAAACTCTCA CTTGGCAGCT 
CATGTGGATG ACTTTGAGCG GCGAAACCAG GACTACAAAG ACATCTGTGC ACGCATGAGT 
TTCCACCTGC TGGAATACCC GCCTGTCAGT ATAGTGAAAC ATGATGTGGT GATTTGGCTT 
GGAGATCTCA ACTACAGGCT ATGCTTGCCT GATGCTGGGG AGGTAAAAAG GCTTATTGCT 
GAAAAAGAGC TACGGAGACT ACAGGAATAT GACCAGCTGA ACCTCCAGAG AAAGACTAAA 
CGTGTCTTCA CCGATTTCAT GGAAGGGGAG ATCAATTTCA TCCCAACTTA CAAATATGAC 
GCTAAATCAG ATCGGTGGGA CTCAAGTGGG AAGTGTCGGA TTCCTGCTTG GTGCGACCGT 
ATCCTATGGA GGGGAAGCAA CGTGAAGCAG CTTCACTACC GCAGTCACAT GGAGCTGAAG 
ACCAGTGACC ACAAACCTGT CAGCTCTGTC TTTAGTATCG GGGTGAAGAT GGTGATTGAG 
CAACGCTACA AAAAGATTTT CGAAGAGATT GTGCGGGACA TGGACAGGAT GGAAAATGAC 
TTTCTGCCCT CTTTGTCCCT AACCCGACGG GAGTTCACCT TTGAGAATGT GAAGTTCCGT 
CAGCTGCAGC GTCAGAGTTT TCTTATCACT AATGATGGAC AAGTTGCTTG CACCTTCGCC 
TTTATTCCCA AACTCAATGA CTCCCAATAC TGTAAGCCCT GGTTACGTGC TGAGCCCTGT 
GAAGGGGTAC TAGATCCTAA TGAGAAAATC GAGATCTTCT TGGAGGTGTA TGTCAGTAAA 
GACTCAGTGA CGCTTTTAAA CTCTGGTGAA GACAACATTG AGGACATCCT GGTGCTTCAT 
CTGGACAGAG GGAAAGACTA CTTCATCACT GTCTCTGGAA ACTACCTGCC CAGTTGTTTC 
GGCACATCTC TGGAGACTCT GTGCCGCATG AAGAAACCTA TCCGTGAGAT CCCCATCACC 
AAACTCATCG ACCTGGAGAA AGCCCGGATG ACATTCTTGT TGGACAGTGC TGGAACAGAA 
GATAAACCTC TAAAGATTCC CAAAGAGGTT TGGTTGCTTG TTAATCACCT CTACACCAAG 
GCCTGCCAGC AGGAGGACCT TTTCCAGACC CCTGGCCTGC AAGACGAGCT ACAAAGTATT 
ATTGACTGCT TGGACACCAG CATTCCTGAT TCCATCCCTG GCTGTAATCA CTCGGTTGCT 
GAAGCTCTAT TGATCTTCTT GGAGGCTCTT CCTGAACCCG TGTTGTGTTA TGAACTCTTT 
CAGCGCTGTC TCGAATGCTC TCATGACAGC CGCCTCTGCA AGCAGCTAAT ATCCCAGCTC 
CCACGAGCCC ATCGCAATGT GTTCCGCTAC CTGATGGCCT TCTTGAGAGA ATTACTCAAA 
CACTCTATTG ACAACAACCT AAACGCCAAC CTCCTAGCTA CTCTCTTCGC AAGCCTCCTC 
ATCCGACCGC CTCCCAACCT TGCTGGCAAA CAGACGCAAC ATGATCGCCA GAAAGCCAAC 
GACTTCATCC TGGGTTTTCT GATGGCAGGC GACGAAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OCRL ( XM_009291139.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009289414.1 Danio rerio oculocerebrorenal syndrome of Lowe (ocrl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa46586
Clone ID Related Accession (Same CDS sequence)	XM_009291139.3 , XM_009291139.2
Accession Version	XM_009291139.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	inositol polyphosphate 5-phosphatase OCRL-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291139.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAC CTTCATTTGG GATTTCCGAA AGCCAGTGTA TAGCCACTGT TAGAGGACAT 
GAAATCAGAA GCGGACAGAA AGAACCAAGA CTCCTGTCCT TGTTAGAGTG TTCTGGGCAG 
TTCAAGTTGG ATATCCTTTC AAATCCTGAT GCAATTGTAT TAAGCCCGGT GTCCCAGCTG 
TCCATCCCCA TCAATAACCA TTTTCGGATC ATTCGAGAGG CAGAAGAAGC TCTTCTCATT 
GACATTGACT CTATTAGCAA GACAGTACGA ATACGTCTGC GAGGTGAACG AACACCGGAG 
CTTCTGCTTG AGCTTCAGGA TGACGGGCAA ACGCAGTCTT TTCTTGAGCA TGTGACGAAG 
GCCAAACAGC AAGCTGAGAG GAGTTCACTG CCCAGGAAGA TGGAGCCAGT TGTACCTGCT 
CCAGTTAAAG ACTGTGTTCC AGTTCCACCT CAGAGAGGAA CCTCCAAATC CTTCTCAAAC 
AACATCAACA ACAGCACTTT AGCAAATCCC TCAAACACAG ATTCCACCCG CTCGCTTGCC 
ACAGATTTTG GCTTTGAAGA AAACTTTAAC CACGTTTTAA AAGTGGAAGA GAAGAAGAAC 
AAGCAGAATC GTTTAGTTGC ACAACGAGAG CCACCACCTC CACCAATTCC CCCTCTTCCT 
CCTAGGAAAG GCACAAATAC ATCATTGACA GGCCCAGCTA CCAAAGAAAG GCCACCATCT 
GTACAAAATG CCACACCTGT CAGAAATGAA TTTCAGTCTG TAGACCGATC TGTTTCCTGG 
TCTGAAAGCC CGTCCAACTC CACCATAAGA CAGGCCATGA TGAGCAGCCA TGCAGGGTCA 
AGAGAAGGTT TGCTCAAGTA TCGCCTCAGC AAGAAAGAGA AAGAATATGT GGACATTAAG 
AACTTTAAGT TCTTCGTAGG CACGTGGAAT GTAAATGGTC AGTCCCCTGA CAGCAGTCTT 
GGGCCCTGGC TCAGTTCTGA CCCAGACCCT CCTGACGTCT ATGCACTAGG ATTTCAGGAG 
CTGGACCTGA GCACAGAGGC GTTTTTCTAT ATGGACTCAT CCAAAGAACA GTTATGGGTT 
GATGCAGTAG AGAGAGGCCT CCATCAAAAA GCCAGATACA TTCAGGTTCG TATTATCCGC 
CTAGTGGGCA TGATGTTGGT GGTGTATATC AAAAAGGAGC ACAAAGATCA AATTCGAGAG 
ATCGCTTCTG AGTCTGTGGG AACTGGCCTC ATGAATAAAA TGGGGAACAA GGGAGGAGTG 
GCAGTGCGCT TTGTGTTTCA CAATACCAGC TTCTGCTTCG TAAACTCTCA CTTGGCAGCT 
CATGTGGATG ACTTTGAGCG GCGAAACCAG GACTACAAAG ACATCTGTGC ACGCATGAGT 
TTCCACCTGC TGGAATACCC GCCTGTCAGT ATAGTGAAAC ATGATGTGGT GATTTGGCTT 
GGAGATCTCA ACTACAGGCT ATGCTTGCCT GATGCTGGGG AGGTAAAAAG GCTTATTGCT 
GAAAAAGAGC TACGGAGACT ACAGGAATAT GACCAGCTGA ACCTCCAGAG AAAGACTAAA 
CGTGTCTTCA CCGATTTCAT GGAAGGGGAG ATCAATTTCA TCCCAACTTA CAAATATGAC 
GCTAAATCAG ATCGGTGGGA CTCAAGTGGG AAGTGTCGGA TTCCTGCTTG GTGCGACCGT 
ATCCTATGGA GGGGAAGCAA CGTGAAGCAG CTTCACTACC GCAGTCACAT GGAGCTGAAG 
ACCAGTGACC ACAAACCTGT CAGCTCTGTC TTTAGTATCG GGGTGAAGAT GGTGATTGAG 
CAACGCTACA AAAAGATTTT CGAAGAGATT GTGCGGGACA TGGACAGGAT GGAAAATGAC 
TTTCTGCCCT CTTTGTCCCT AACCCGACGG GAGTTCACCT TTGAGAATGT GAAGTTCCGT 
CAGCTGCAGC GTCAGAGTTT TCTTATCACT AATGATGGAC AAGTTGCTTG CACCTTCGCC 
TTTATTCCCA AACTCAATGA CTCCCAATAC TGTAAGCCCT GGTTACGTGC TGAGCCCTGT 
GAAGGGGTAC TAGATCCTAA TGAGAAAATC GAGATCTTCT TGGAGGTGTA TGTCAGTAAA 
GACTCAGTGA CGCTTTTAAA CTCTGGTGAA GACAACATTG AGGACATCCT GGTGCTTCAT 
CTGGACAGAG GGAAAGACTA CTTCATCACT GTCTCTGGAA ACTACCTGCC CAGTTGTTTC 
GGCACATCTC TGGAGACTCT GTGCCGCATG AAGAAACCTA TCCGTGAGAT CCCCATCACC 
AAACTCATCG ACCTGGGAGA GGATAGCTGC ATGGAAAAGG AGAAAGCCCG GATGACATTC 
TTGTTGGACA GTGCTGGAAC AGAAGATAAA CCTCTAAAGA TTCCCAAAGA GGTTTGGTTG 
CTTGTTAATC ACCTCTACAC CAAGGCCTGC CAGCAGGAGG ACCTTTTCCA GACCCCTGGC 
CTGCAAGACG AGCTACAAAG TATTATTGAC TGCTTGGACA CCAGCATTCC TGATTCCATC 
CCTGGCTGTA ATCACTCGGT TGCTGAAGCT CTATTGATCT TCTTGGAGGC TCTTCCTGAA 
CCCGTGTTGT GTTATGAACT CTTTCAGCGC TGTCTCGAAT GCTCTCATGA CAGCCGCCTC 
TGCAAGCAGC TAATATCCCA GCTCCCACGA GCCCATCGCA ATGTGTTCCG CTACCTGATG 
GCCTTCTTGA GAGAATTACT CAAACACTCT ATTGACAACA ACCTAAACGC CAACCTCCTA 
GCTACTCTCT TCGCAAGCCT CCTCATCCGA CCGCCTCCCA ACCTTGCTGG CAAACAGACG 
CAACATGATC GCCAGAAAGC CAACGACTTC ATCCTGGGTT TTCTGATGGC AGGCGACGAA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289414.1
CDS	330..3215
Translation	MMEPSFGISESQCIATVRGHEIRSGQKEPRLLSLLECSGQFKLDILSNPDAIVLSPVSQLSIPINNHFRIIREAEEALLIDIDSISKTVRIRLRGERTPELLLELQDDGQTQSFLEHVTKAKQQAERSSLPRKMEPVVPAPVKDCVPVPPQRGTSKSFSNNINNSTLANPSNTDSTRSLATDFGFEENFNHVLKVEEKKNKQNRLVAQREPPPPPIPPLPPRKGTNTSLTGPATKERPPSVQNATPVRNEFQSVDRSVSWSESPSNSTIRQAMMSSHAGSREGLLKYRLSKKEKEYVDIKNFKFFVGTWNVNGQSPDSSLGPWLSSDPDPPDVYALGFQELDLSTEAFFYMDSSKEQLWVDAVERGLHQKARYIQVRIIRLVGMMLVVYIKKEHKDQIREIASESVGTGLMNKMGNKGGVAVRFVFHNTSFCFVNSHLAAHVDDFERRNQDYKDICARMSFHLLEYPPVSIVKHDVVIWLGDLNYRLCLPDAGEVKRLIAEKELRRLQEYDQLNLQRKTKRVFTDFMEGEINFIPTYKYDAKSDRWDSSGKCRIPAWCDRILWRGSNVKQLHYRSHMELKTSDHKPVSSVFSIGVKMVIEQRYKKIFEEIVRDMDRMENDFLPSLSLTRREFTFENVKFRQLQRQSFLITNDGQVACTFAFIPKLNDSQYCKPWLRAEPCEGVLDPNEKIEIFLEVYVSKDSVTLLNSGEDNIEDILVLHLDRGKDYFITVSGNYLPSCFGTSLETLCRMKKPIREIPITKLIDLGEDSCMEKEKARMTFLLDSAGTEDKPLKIPKEVWLLVNHLYTKACQQEDLFQTPGLQDELQSIIDCLDTSIPDSIPGCNHSVAEALLIFLEALPEPVLCYELFQRCLECSHDSRLCKQLISQLPRAHRNVFRYLMAFLRELLKHSIDNNLNANLLATLFASLLIRPPPNLAGKQTQHDRQKANDFILGFLMAGDED

Target ORF information:

RefSeq Version	XM_009291139.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio oculocerebrorenal syndrome of Lowe (ocrl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291139.3

ORF Insert Sequence:

ATGATGGAAC CTTCATTTGG GATTTCCGAA AGCCAGTGTA TAGCCACTGT TAGAGGACAT 
GAAATCAGAA GCGGACAGAA AGAACCAAGA CTCCTGTCCT TGTTAGAGTG TTCTGGGCAG 
TTCAAGTTGG ATATCCTTTC AAATCCTGAT GCAATTGTAT TAAGCCCGGT GTCCCAGCTG 
TCCATCCCCA TCAATAACCA TTTTCGGATC ATTCGAGAGG CAGAAGAAGC TCTTCTCATT 
GACATTGACT CTATTAGCAA GACAGTACGA ATACGTCTGC GAGGTGAACG AACACCGGAG 
CTTCTGCTTG AGCTTCAGGA TGACGGGCAA ACGCAGTCTT TTCTTGAGCA TGTGACGAAG 
GCCAAACAGC AAGCTGAGAG GAGTTCACTG CCCAGGAAGA TGGAGCCAGT TGTACCTGCT 
CCAGTTAAAG ACTGTGTTCC AGTTCCACCT CAGAGAGGAA CCTCCAAATC CTTCTCAAAC 
AACATCAACA ACAGCACTTT AGCAAATCCC TCAAACACAG ATTCCACCCG CTCGCTTGCC 
ACAGATTTTG GCTTTGAAGA AAACTTTAAC CACGTTTTAA AAGTGGAAGA GAAGAAGAAC 
AAGCAGAATC GTTTAGTTGC ACAACGAGAG CCACCACCTC CACCAATTCC CCCTCTTCCT 
CCTAGGAAAG GCACAAATAC ATCATTGACA GGCCCAGCTA CCAAAGAAAG GCCACCATCT 
GTACAAAATG CCACACCTGT CAGAAATGAA TTTCAGTCTG TAGACCGATC TGTTTCCTGG 
TCTGAAAGCC CGTCCAACTC CACCATAAGA CAGGCCATGA TGAGCAGCCA TGCAGGGTCA 
AGAGAAGGTT TGCTCAAGTA TCGCCTCAGC AAGAAAGAGA AAGAATATGT GGACATTAAG 
AACTTTAAGT TCTTCGTAGG CACGTGGAAT GTAAATGGTC AGTCCCCTGA CAGCAGTCTT 
GGGCCCTGGC TCAGTTCTGA CCCAGACCCT CCTGACGTCT ATGCACTAGG ATTTCAGGAG 
CTGGACCTGA GCACAGAGGC GTTTTTCTAT ATGGACTCAT CCAAAGAACA GTTATGGGTT 
GATGCAGTAG AGAGAGGCCT CCATCAAAAA GCCAGATACA TTCAGGTTCG TATTATCCGC 
CTAGTGGGCA TGATGTTGGT GGTGTATATC AAAAAGGAGC ACAAAGATCA AATTCGAGAG 
ATCGCTTCTG AGTCTGTGGG AACTGGCCTC ATGAATAAAA TGGGGAACAA GGGAGGAGTG 
GCAGTGCGCT TTGTGTTTCA CAATACCAGC TTCTGCTTCG TAAACTCTCA CTTGGCAGCT 
CATGTGGATG ACTTTGAGCG GCGAAACCAG GACTACAAAG ACATCTGTGC ACGCATGAGT 
TTCCACCTGC TGGAATACCC GCCTGTCAGT ATAGTGAAAC ATGATGTGGT GATTTGGCTT 
GGAGATCTCA ACTACAGGCT ATGCTTGCCT GATGCTGGGG AGGTAAAAAG GCTTATTGCT 
GAAAAAGAGC TACGGAGACT ACAGGAATAT GACCAGCTGA ACCTCCAGAG AAAGACTAAA 
CGTGTCTTCA CCGATTTCAT GGAAGGGGAG ATCAATTTCA TCCCAACTTA CAAATATGAC 
GCTAAATCAG ATCGGTGGGA CTCAAGTGGG AAGTGTCGGA TTCCTGCTTG GTGCGACCGT 
ATCCTATGGA GGGGAAGCAA CGTGAAGCAG CTTCACTACC GCAGTCACAT GGAGCTGAAG 
ACCAGTGACC ACAAACCTGT CAGCTCTGTC TTTAGTATCG GGGTGAAGAT GGTGATTGAG 
CAACGCTACA AAAAGATTTT CGAAGAGATT GTGCGGGACA TGGACAGGAT GGAAAATGAC 
TTTCTGCCCT CTTTGTCCCT AACCCGACGG GAGTTCACCT TTGAGAATGT GAAGTTCCGT 
CAGCTGCAGC GTCAGAGTTT TCTTATCACT AATGATGGAC AAGTTGCTTG CACCTTCGCC 
TTTATTCCCA AACTCAATGA CTCCCAATAC TGTAAGCCCT GGTTACGTGC TGAGCCCTGT 
GAAGGGGTAC TAGATCCTAA TGAGAAAATC GAGATCTTCT TGGAGGTGTA TGTCAGTAAA 
GACTCAGTGA CGCTTTTAAA CTCTGGTGAA GACAACATTG AGGACATCCT GGTGCTTCAT 
CTGGACAGAG GGAAAGACTA CTTCATCACT GTCTCTGGAA ACTACCTGCC CAGTTGTTTC 
GGCACATCTC TGGAGACTCT GTGCCGCATG AAGAAACCTA TCCGTGAGAT CCCCATCACC 
AAACTCATCG ACCTGGGAGA GGATAGCTGC ATGGAAAAGG AGAAAGCCCG GATGACATTC 
TTGTTGGACA GTGCTGGAAC AGAAGATAAA CCTCTAAAGA TTCCCAAAGA GGTTTGGTTG 
CTTGTTAATC ACCTCTACAC CAAGGCCTGC CAGCAGGAGG ACCTTTTCCA GACCCCTGGC 
CTGCAAGACG AGCTACAAAG TATTATTGAC TGCTTGGACA CCAGCATTCC TGATTCCATC 
CCTGGCTGTA ATCACTCGGT TGCTGAAGCT CTATTGATCT TCTTGGAGGC TCTTCCTGAA 
CCCGTGTTGT GTTATGAACT CTTTCAGCGC TGTCTCGAAT GCTCTCATGA CAGCCGCCTC 
TGCAAGCAGC TAATATCCCA GCTCCCACGA GCCCATCGCA ATGTGTTCCG CTACCTGATG 
GCCTTCTTGA GAGAATTACT CAAACACTCT ATTGACAACA ACCTAAACGC CAACCTCCTA 
GCTACTCTCT TCGCAAGCCT CCTCATCCGA CCGCCTCCCA ACCTTGCTGG CAAACAGACG 
CAACATGATC GCCAGAAAGC CAACGACTTC ATCCTGGGTT TTCTGATGGC AGGCGACGAA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OCRL ( XM_009291139.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009289414.1 Danio rerio oculocerebrorenal syndrome of Lowe (ocrl), transcript variant X1, mRNA.

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CloneID	ODa46586
Clone ID Related Accession (Same CDS sequence)	XM_009291139.3 , XM_009291139.2
Accession Version	XM_009291139.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	inositol polyphosphate 5-phosphatase OCRL-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291139.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAC CTTCATTTGG GATTTCCGAA AGCCAGTGTA TAGCCACTGT TAGAGGACAT 
GAAATCAGAA GCGGACAGAA AGAACCAAGA CTCCTGTCCT TGTTAGAGTG TTCTGGGCAG 
TTCAAGTTGG ATATCCTTTC AAATCCTGAT GCAATTGTAT TAAGCCCGGT GTCCCAGCTG 
TCCATCCCCA TCAATAACCA TTTTCGGATC ATTCGAGAGG CAGAAGAAGC TCTTCTCATT 
GACATTGACT CTATTAGCAA GACAGTACGA ATACGTCTGC GAGGTGAACG AACACCGGAG 
CTTCTGCTTG AGCTTCAGGA TGACGGGCAA ACGCAGTCTT TTCTTGAGCA TGTGACGAAG 
GCCAAACAGC AAGCTGAGAG GAGTTCACTG CCCAGGAAGA TGGAGCCAGT TGTACCTGCT 
CCAGTTAAAG ACTGTGTTCC AGTTCCACCT CAGAGAGGAA CCTCCAAATC CTTCTCAAAC 
AACATCAACA ACAGCACTTT AGCAAATCCC TCAAACACAG ATTCCACCCG CTCGCTTGCC 
ACAGATTTTG GCTTTGAAGA AAACTTTAAC CACGTTTTAA AAGTGGAAGA GAAGAAGAAC 
AAGCAGAATC GTTTAGTTGC ACAACGAGAG CCACCACCTC CACCAATTCC CCCTCTTCCT 
CCTAGGAAAG GCACAAATAC ATCATTGACA GGCCCAGCTA CCAAAGAAAG GCCACCATCT 
GTACAAAATG CCACACCTGT CAGAAATGAA TTTCAGTCTG TAGACCGATC TGTTTCCTGG 
TCTGAAAGCC CGTCCAACTC CACCATAAGA CAGGCCATGA TGAGCAGCCA TGCAGGGTCA 
AGAGAAGGTT TGCTCAAGTA TCGCCTCAGC AAGAAAGAGA AAGAATATGT GGACATTAAG 
AACTTTAAGT TCTTCGTAGG CACGTGGAAT GTAAATGGTC AGTCCCCTGA CAGCAGTCTT 
GGGCCCTGGC TCAGTTCTGA CCCAGACCCT CCTGACGTCT ATGCACTAGG ATTTCAGGAG 
CTGGACCTGA GCACAGAGGC GTTTTTCTAT ATGGACTCAT CCAAAGAACA GTTATGGGTT 
GATGCAGTAG AGAGAGGCCT CCATCAAAAA GCCAGATACA TTCAGGTTCG TATTATCCGC 
CTAGTGGGCA TGATGTTGGT GGTGTATATC AAAAAGGAGC ACAAAGATCA AATTCGAGAG 
ATCGCTTCTG AGTCTGTGGG AACTGGCCTC ATGAATAAAA TGGGGAACAA GGGAGGAGTG 
GCAGTGCGCT TTGTGTTTCA CAATACCAGC TTCTGCTTCG TAAACTCTCA CTTGGCAGCT 
CATGTGGATG ACTTTGAGCG GCGAAACCAG GACTACAAAG ACATCTGTGC ACGCATGAGT 
TTCCACCTGC TGGAATACCC GCCTGTCAGT ATAGTGAAAC ATGATGTGGT GATTTGGCTT 
GGAGATCTCA ACTACAGGCT ATGCTTGCCT GATGCTGGGG AGGTAAAAAG GCTTATTGCT 
GAAAAAGAGC TACGGAGACT ACAGGAATAT GACCAGCTGA ACCTCCAGAG AAAGACTAAA 
CGTGTCTTCA CCGATTTCAT GGAAGGGGAG ATCAATTTCA TCCCAACTTA CAAATATGAC 
GCTAAATCAG ATCGGTGGGA CTCAAGTGGG AAGTGTCGGA TTCCTGCTTG GTGCGACCGT 
ATCCTATGGA GGGGAAGCAA CGTGAAGCAG CTTCACTACC GCAGTCACAT GGAGCTGAAG 
ACCAGTGACC ACAAACCTGT CAGCTCTGTC TTTAGTATCG GGGTGAAGAT GGTGATTGAG 
CAACGCTACA AAAAGATTTT CGAAGAGATT GTGCGGGACA TGGACAGGAT GGAAAATGAC 
TTTCTGCCCT CTTTGTCCCT AACCCGACGG GAGTTCACCT TTGAGAATGT GAAGTTCCGT 
CAGCTGCAGC GTCAGAGTTT TCTTATCACT AATGATGGAC AAGTTGCTTG CACCTTCGCC 
TTTATTCCCA AACTCAATGA CTCCCAATAC TGTAAGCCCT GGTTACGTGC TGAGCCCTGT 
GAAGGGGTAC TAGATCCTAA TGAGAAAATC GAGATCTTCT TGGAGGTGTA TGTCAGTAAA 
GACTCAGTGA CGCTTTTAAA CTCTGGTGAA GACAACATTG AGGACATCCT GGTGCTTCAT 
CTGGACAGAG GGAAAGACTA CTTCATCACT GTCTCTGGAA ACTACCTGCC CAGTTGTTTC 
GGCACATCTC TGGAGACTCT GTGCCGCATG AAGAAACCTA TCCGTGAGAT CCCCATCACC 
AAACTCATCG ACCTGGGAGA GGATAGCTGC ATGGAAAAGG AGAAAGCCCG GATGACATTC 
TTGTTGGACA GTGCTGGAAC AGAAGATAAA CCTCTAAAGA TTCCCAAAGA GGTTTGGTTG 
CTTGTTAATC ACCTCTACAC CAAGGCCTGC CAGCAGGAGG ACCTTTTCCA GACCCCTGGC 
CTGCAAGACG AGCTACAAAG TATTATTGAC TGCTTGGACA CCAGCATTCC TGATTCCATC 
CCTGGCTGTA ATCACTCGGT TGCTGAAGCT CTATTGATCT TCTTGGAGGC TCTTCCTGAA 
CCCGTGTTGT GTTATGAACT CTTTCAGCGC TGTCTCGAAT GCTCTCATGA CAGCCGCCTC 
TGCAAGCAGC TAATATCCCA GCTCCCACGA GCCCATCGCA ATGTGTTCCG CTACCTGATG 
GCCTTCTTGA GAGAATTACT CAAACACTCT ATTGACAACA ACCTAAACGC CAACCTCCTA 
GCTACTCTCT TCGCAAGCCT CCTCATCCGA CCGCCTCCCA ACCTTGCTGG CAAACAGACG 
CAACATGATC GCCAGAAAGC CAACGACTTC ATCCTGGGTT TTCTGATGGC AGGCGACGAA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289414.1
CDS	327..3212
Translation	MMEPSFGISESQCIATVRGHEIRSGQKEPRLLSLLECSGQFKLDILSNPDAIVLSPVSQLSIPINNHFRIIREAEEALLIDIDSISKTVRIRLRGERTPELLLELQDDGQTQSFLEHVTKAKQQAERSSLPRKMEPVVPAPVKDCVPVPPQRGTSKSFSNNINNSTLANPSNTDSTRSLATDFGFEENFNHVLKVEEKKNKQNRLVAQREPPPPPIPPLPPRKGTNTSLTGPATKERPPSVQNATPVRNEFQSVDRSVSWSESPSNSTIRQAMMSSHAGSREGLLKYRLSKKEKEYVDIKNFKFFVGTWNVNGQSPDSSLGPWLSSDPDPPDVYALGFQELDLSTEAFFYMDSSKEQLWVDAVERGLHQKARYIQVRIIRLVGMMLVVYIKKEHKDQIREIASESVGTGLMNKMGNKGGVAVRFVFHNTSFCFVNSHLAAHVDDFERRNQDYKDICARMSFHLLEYPPVSIVKHDVVIWLGDLNYRLCLPDAGEVKRLIAEKELRRLQEYDQLNLQRKTKRVFTDFMEGEINFIPTYKYDAKSDRWDSSGKCRIPAWCDRILWRGSNVKQLHYRSHMELKTSDHKPVSSVFSIGVKMVIEQRYKKIFEEIVRDMDRMENDFLPSLSLTRREFTFENVKFRQLQRQSFLITNDGQVACTFAFIPKLNDSQYCKPWLRAEPCEGVLDPNEKIEIFLEVYVSKDSVTLLNSGEDNIEDILVLHLDRGKDYFITVSGNYLPSCFGTSLETLCRMKKPIREIPITKLIDLGEDSCMEKEKARMTFLLDSAGTEDKPLKIPKEVWLLVNHLYTKACQQEDLFQTPGLQDELQSIIDCLDTSIPDSIPGCNHSVAEALLIFLEALPEPVLCYELFQRCLECSHDSRLCKQLISQLPRAHRNVFRYLMAFLRELLKHSIDNNLNANLLATLFASLLIRPPPNLAGKQTQHDRQKANDFILGFLMAGDED

Target ORF information:

RefSeq Version	XM_009291139.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio oculocerebrorenal syndrome of Lowe (ocrl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291139.2

ORF Insert Sequence:

ATGATGGAAC CTTCATTTGG GATTTCCGAA AGCCAGTGTA TAGCCACTGT TAGAGGACAT 
GAAATCAGAA GCGGACAGAA AGAACCAAGA CTCCTGTCCT TGTTAGAGTG TTCTGGGCAG 
TTCAAGTTGG ATATCCTTTC AAATCCTGAT GCAATTGTAT TAAGCCCGGT GTCCCAGCTG 
TCCATCCCCA TCAATAACCA TTTTCGGATC ATTCGAGAGG CAGAAGAAGC TCTTCTCATT 
GACATTGACT CTATTAGCAA GACAGTACGA ATACGTCTGC GAGGTGAACG AACACCGGAG 
CTTCTGCTTG AGCTTCAGGA TGACGGGCAA ACGCAGTCTT TTCTTGAGCA TGTGACGAAG 
GCCAAACAGC AAGCTGAGAG GAGTTCACTG CCCAGGAAGA TGGAGCCAGT TGTACCTGCT 
CCAGTTAAAG ACTGTGTTCC AGTTCCACCT CAGAGAGGAA CCTCCAAATC CTTCTCAAAC 
AACATCAACA ACAGCACTTT AGCAAATCCC TCAAACACAG ATTCCACCCG CTCGCTTGCC 
ACAGATTTTG GCTTTGAAGA AAACTTTAAC CACGTTTTAA AAGTGGAAGA GAAGAAGAAC 
AAGCAGAATC GTTTAGTTGC ACAACGAGAG CCACCACCTC CACCAATTCC CCCTCTTCCT 
CCTAGGAAAG GCACAAATAC ATCATTGACA GGCCCAGCTA CCAAAGAAAG GCCACCATCT 
GTACAAAATG CCACACCTGT CAGAAATGAA TTTCAGTCTG TAGACCGATC TGTTTCCTGG 
TCTGAAAGCC CGTCCAACTC CACCATAAGA CAGGCCATGA TGAGCAGCCA TGCAGGGTCA 
AGAGAAGGTT TGCTCAAGTA TCGCCTCAGC AAGAAAGAGA AAGAATATGT GGACATTAAG 
AACTTTAAGT TCTTCGTAGG CACGTGGAAT GTAAATGGTC AGTCCCCTGA CAGCAGTCTT 
GGGCCCTGGC TCAGTTCTGA CCCAGACCCT CCTGACGTCT ATGCACTAGG ATTTCAGGAG 
CTGGACCTGA GCACAGAGGC GTTTTTCTAT ATGGACTCAT CCAAAGAACA GTTATGGGTT 
GATGCAGTAG AGAGAGGCCT CCATCAAAAA GCCAGATACA TTCAGGTTCG TATTATCCGC 
CTAGTGGGCA TGATGTTGGT GGTGTATATC AAAAAGGAGC ACAAAGATCA AATTCGAGAG 
ATCGCTTCTG AGTCTGTGGG AACTGGCCTC ATGAATAAAA TGGGGAACAA GGGAGGAGTG 
GCAGTGCGCT TTGTGTTTCA CAATACCAGC TTCTGCTTCG TAAACTCTCA CTTGGCAGCT 
CATGTGGATG ACTTTGAGCG GCGAAACCAG GACTACAAAG ACATCTGTGC ACGCATGAGT 
TTCCACCTGC TGGAATACCC GCCTGTCAGT ATAGTGAAAC ATGATGTGGT GATTTGGCTT 
GGAGATCTCA ACTACAGGCT ATGCTTGCCT GATGCTGGGG AGGTAAAAAG GCTTATTGCT 
GAAAAAGAGC TACGGAGACT ACAGGAATAT GACCAGCTGA ACCTCCAGAG AAAGACTAAA 
CGTGTCTTCA CCGATTTCAT GGAAGGGGAG ATCAATTTCA TCCCAACTTA CAAATATGAC 
GCTAAATCAG ATCGGTGGGA CTCAAGTGGG AAGTGTCGGA TTCCTGCTTG GTGCGACCGT 
ATCCTATGGA GGGGAAGCAA CGTGAAGCAG CTTCACTACC GCAGTCACAT GGAGCTGAAG 
ACCAGTGACC ACAAACCTGT CAGCTCTGTC TTTAGTATCG GGGTGAAGAT GGTGATTGAG 
CAACGCTACA AAAAGATTTT CGAAGAGATT GTGCGGGACA TGGACAGGAT GGAAAATGAC 
TTTCTGCCCT CTTTGTCCCT AACCCGACGG GAGTTCACCT TTGAGAATGT GAAGTTCCGT 
CAGCTGCAGC GTCAGAGTTT TCTTATCACT AATGATGGAC AAGTTGCTTG CACCTTCGCC 
TTTATTCCCA AACTCAATGA CTCCCAATAC TGTAAGCCCT GGTTACGTGC TGAGCCCTGT 
GAAGGGGTAC TAGATCCTAA TGAGAAAATC GAGATCTTCT TGGAGGTGTA TGTCAGTAAA 
GACTCAGTGA CGCTTTTAAA CTCTGGTGAA GACAACATTG AGGACATCCT GGTGCTTCAT 
CTGGACAGAG GGAAAGACTA CTTCATCACT GTCTCTGGAA ACTACCTGCC CAGTTGTTTC 
GGCACATCTC TGGAGACTCT GTGCCGCATG AAGAAACCTA TCCGTGAGAT CCCCATCACC 
AAACTCATCG ACCTGGGAGA GGATAGCTGC ATGGAAAAGG AGAAAGCCCG GATGACATTC 
TTGTTGGACA GTGCTGGAAC AGAAGATAAA CCTCTAAAGA TTCCCAAAGA GGTTTGGTTG 
CTTGTTAATC ACCTCTACAC CAAGGCCTGC CAGCAGGAGG ACCTTTTCCA GACCCCTGGC 
CTGCAAGACG AGCTACAAAG TATTATTGAC TGCTTGGACA CCAGCATTCC TGATTCCATC 
CCTGGCTGTA ATCACTCGGT TGCTGAAGCT CTATTGATCT TCTTGGAGGC TCTTCCTGAA 
CCCGTGTTGT GTTATGAACT CTTTCAGCGC TGTCTCGAAT GCTCTCATGA CAGCCGCCTC 
TGCAAGCAGC TAATATCCCA GCTCCCACGA GCCCATCGCA ATGTGTTCCG CTACCTGATG 
GCCTTCTTGA GAGAATTACT CAAACACTCT ATTGACAACA ACCTAAACGC CAACCTCCTA 
GCTACTCTCT TCGCAAGCCT CCTCATCCGA CCGCCTCCCA ACCTTGCTGG CAAACAGACG 
CAACATGATC GCCAGAAAGC CAACGACTTC ATCCTGGGTT TTCTGATGGC AGGCGACGAA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Apela Regulates Fluid Homeostasis by Binding to the APJ Receptor to Activate Gi Signaling.
The Journal of biological chemistry290(30)18261-8(2015 Jul)
Deng C,Chen H,Yang N,Feng Y,Hsueh AJ

The Lowe syndrome protein OCRL1 is required for endocytosis in the zebrafish pronephric tubule.
PLoS genetics11(4)e1005058(2015 Apr)
Oltrabella F,Pietka G,Ramirez IB,Mironov A,Starborg T,Drummond IA,Hinchliffe KA,Lowe M

Compensatory Role of Inositol 5-Phosphatase INPP5B to OCRL in Primary Cilia Formation in Oculocerebrorenal Syndrome of Lowe.
PloS one8(6)e66727(2013)
Luo N,Kumar A,Conwell M,Weinreb RN,Anderson R,Sun Y

Lowe syndrome: Between primary cilia assembly and Rac1-mediated membrane remodeling.
Communicative & integrative biology5(6)641-4(2012 Nov)
Madhivanan K,Mukherjee D,Aguilar RC

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OCRL cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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