me1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	me1
Entrez Gene ID	768196
Full Name	malic enzyme 1, NADP(+)-dependent, cytosolic
Synonyms	zgc:153079
General protein information	Preferred Names malic enzyme 1, NADP(+)-dependent, cytosolic Names NADP-dependent malic enzyme
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005158369.4	XP_005158426.1	NADP-dependent malic enzyme isoform X1
XM_017351366.1	XP_017206855.1	NADP-dependent malic enzyme isoform X1
NM_001310037.1	NP_001296966.1	NADP-dependent malic enzyme
XM_005158369.3	XP_005158426.1	NADP-dependent malic enzyme isoform X1
XM_017351366.2	XP_017206855.1	NADP-dependent malic enzyme isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

SREBP-1 and LXRα pathways mediated Cu-induced hepatic lipid metabolism in zebrafish Danio rerio.
Pan YX, Zhuo MQ, Li DD, Xu YH, Wu K, Luo Z
Chemosphere215370-379(2019 Jan)

Oxidative stress and mitochondrial dysfunction mediated Cd-induced hepatic lipid accumulation in zebrafish Danio rerio.
Pan YX, Luo Z, Zhuo MQ, Wei CC, Chen GH, Song YF
Aquatic toxicology (Amsterdam, Netherlands)19912-20(2018 Jun)

Transforming growth factor-β1 regulates the nascent hematopoietic stem cell niche by promoting gluconeogenesis.
Zhang CY, Yin HM, Wang H, Su D, Xia Y, Yan LF, Fang B, Liu W, Wang YM, Gu AH, Zhou Y
Leukemia32(2)479-491(2018 02)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Spatiotemporal expression and transcriptional regulation of heme oxygenase and biliverdin reductase genes in zebrafish (Danio rerio) suggest novel roles during early developmental periods of heightened oxidative stress.
Holowiecki A, O'Shields B, Jenny MJ
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP191138-151(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following me1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the me1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa46572	NM_001310037.1 Latest version!	Danio rerio malic enzyme 1, NADP(+)-dependent, cytosolic (me1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa21666	XM_017351366.2 Latest version!	Danio rerio malic enzyme 1, NADP(+)-dependent, cytosolic (me1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa21666	XM_005158369.4 Latest version!	Danio rerio malic enzyme 1, NADP(+)-dependent, cytosolic (me1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
ODa21666	XM_005158369.3	Danio rerio malic enzyme 1, NADP(+)-dependent, cytosolic (me1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa21666	XM_017351366.1	Danio rerio malic enzyme 1, NADP(+)-dependent, cytosolic (me1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

me1 ( NM_001310037.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001296966.1 Danio rerio malic enzyme 1, NADP(+)-dependent, cytosolic (me1), mRNA.

Price & Availability↑

CloneID	ODa46572
Clone ID Related Accession (Same CDS sequence)	NM_001310037.1
Accession Version	NM_001310037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553270400000
Organism	Danio rerio(zebrafish)
Product	NADP-dependent malic enzyme
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC152078.1. On Jun 20, 2015 this sequence version replaced XM_005158367.2. ##Evidence-Data-START## Transcript exon combination :: BC152078.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505384, SAMEA4476734 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAAGT CAACTGACCA AAGCAAGACC TTTGTTTACA CAAAGAAGCG GGGGTATGAT 
GTTACACGCA ACCCCCATCT GAATAAGGGG ATGGCCTTCT CCCTGGAGGA GCGCTTGCAG 
CTGGGCATTC ATGGCCTGTT GCCACCCTGC TTCCTCAGCC AGGATGTGCA AGTTTTGCGG 
GTCCTTAGGA GCTACGAGCT GTTAAAGGAA GATCTGGACA GGTATGTCTT TCTAATGGGC 
CTTCAGGACC GTAACGAGAA GCTGTTCTAC CGGGTGCTGA CCTCAGACAT AGAGAGATTC 
ATGCCAATTG TGTACACACC TACCGTTGGC CTAGCGTGCC AACAGTATGG CCTGACCTTC 
AGGAGACCGA GGGGCCTTTT TATTACAATT CATGACCGCT CCCACATCCC CACACTTCTT 
CAGAGCTGGC CGGAGAAGGA CATTCGTGCT GTAGTGGTGA CAGATGGAGA GAGGATTCTG 
GGCCTGGGGG ATCTGGGCTG CTATGGCATG GGCATCCCTG TGGGTAAACT GGCCCTGTAC 
ACTGCCTGTG GAGGAATGCC GCCTCAGCAG TGCCTGCCTG TTATGCTGGA CGTCGGCACA 
GACAAAGAGG AACTTCTGAA GGACCCACTG TACATTGGTT TGAGGCATAA AAGAGTACGT 
GGACAGGCCT ACGATGAGCT TCTGGATGAA TTTATGAAGG CCATCACAGA CAGGTATGGG 
ATGAAATGCC TTATCCAGTT TGAAGATTTT GCCAACATTA ATGCCTTCCG CCTGTTGAGT 
AAATACCGCA ACAAATACCT CACATTCAAT GATGACATCC AAGGCACTGC TGCTGTGGCT 
GTGGCAGGCC TCCTAGCTGC CCTTCGCATT ACCAAAAGCA AAATGTGTGA CCATACCATT 
GTGTTTCAGG GTGCAGGCGA GGCTGCAATG GGCATAGGTG AGCTGATTGT CATGGCAATG 
GAGAAAGAGG GTCTTCCTCG TGAGCAGTGT ATAAAGAAAA TCTGGATGGT AGACTCTAAA 
GGACTTATAG TTAAGGGTCG AGATCACCTC ACCCATGAGA AAGAGAGGTT TGCACATGAG 
CATGCACAAA TGAAAAAGCT TGAGGATGTT GTAAAGGAGC TGAAACCCAC AGCTATAATT 
GGGGTGGCAG CAATCGCCGG CGCTTTCACA GAAGAGATAA TCAAGGCTAT GGCATCCTTC 
AATAAGAATC CTATTATCTT CGCTCTCAGC AACCCCACCA GCAAAGCTGA GTGTACAGCT 
GAACAGTGCT ACACACTCAC TGAGGGAAGG GGGATATTTG CAAGCGGAAG TCCTTTTGAT 
CCAGTAACTC TTCCCGATGG GAGGAAATTC TACCCTGGTC AGGGAAACAA CTCGTATGTG 
TTCCCTGGAG TGGGGCTGGG AGTCACTGCT TGTGAAATGA GACACATCCC TGAGGATGTT 
TTCCTTGTTA CAGCTGAGGC TATAGCAGAA CTGGTGACTG ACAAGGATCT CGCAGAGGGT 
CGTCTGTATC CTCCTCTCAG CAGCATTCGG GAAGTTTCCA TCAAACTGGC TGTGAAGATA 
GTGGAGTATG CATATAAGCA CAAAATGGCT ACGCTGCACC CAGAGCCGCA AGATAAAGAT 
GCTTTCATCC GTTCACTCAT CTATAGCACA GACTACCAAG AGTTTGCTGT GGACTCATAC 
AGCTGGCCTG AGAACGCCAT GACCGTCCAA ACATGTAAAC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296966.1
CDS	29..1753
Translation	MQKSTDQSKTFVYTKKRGYDVTRNPHLNKGMAFSLEERLQLGIHGLLPPCFLSQDVQVLRVLRSYELLKEDLDRYVFLMGLQDRNEKLFYRVLTSDIERFMPIVYTPTVGLACQQYGLTFRRPRGLFITIHDRSHIPTLLQSWPEKDIRAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGMPPQQCLPVMLDVGTDKEELLKDPLYIGLRHKRVRGQAYDELLDEFMKAITDRYGMKCLIQFEDFANINAFRLLSKYRNKYLTFNDDIQGTAAVAVAGLLAALRITKSKMCDHTIVFQGAGEAAMGIGELIVMAMEKEGLPREQCIKKIWMVDSKGLIVKGRDHLTHEKERFAHEHAQMKKLEDVVKELKPTAIIGVAAIAGAFTEEIIKAMASFNKNPIIFALSNPTSKAECTAEQCYTLTEGRGIFASGSPFDPVTLPDGRKFYPGQGNNSYVFPGVGLGVTACEMRHIPEDVFLVTAEAIAELVTDKDLAEGRLYPPLSSIREVSIKLAVKIVEYAYKHKMATLHPEPQDKDAFIRSLIYSTDYQEFAVDSYSWPENAMTVQTCKL

Target ORF information:

RefSeq Version	NM_001310037.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio malic enzyme 1, NADP(+)-dependent, cytosolic (me1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310037.1

ORF Insert Sequence:

ATGCAGAAGT CAACTGACCA AAGCAAGACC TTTGTTTACA CAAAGAAGCG GGGGTATGAT 
GTTACACGCA ACCCCCATCT GAATAAGGGG ATGGCCTTCT CCCTGGAGGA GCGCTTGCAG 
CTGGGCATTC ATGGCCTGTT GCCACCCTGC TTCCTCAGCC AGGATGTGCA AGTTTTGCGG 
GTCCTTAGGA GCTACGAGCT GTTAAAGGAA GATCTGGACA GGTATGTCTT TCTAATGGGC 
CTTCAGGACC GTAACGAGAA GCTGTTCTAC CGGGTGCTGA CCTCAGACAT AGAGAGATTC 
ATGCCAATTG TGTACACACC TACCGTTGGC CTAGCGTGCC AACAGTATGG CCTGACCTTC 
AGGAGACCGA GGGGCCTTTT TATTACAATT CATGACCGCT CCCACATCCC CACACTTCTT 
CAGAGCTGGC CGGAGAAGGA CATTCGTGCT GTAGTGGTGA CAGATGGAGA GAGGATTCTG 
GGCCTGGGGG ATCTGGGCTG CTATGGCATG GGCATCCCTG TGGGTAAACT GGCCCTGTAC 
ACTGCCTGTG GAGGAATGCC GCCTCAGCAG TGCCTGCCTG TTATGCTGGA CGTCGGCACA 
GACAAAGAGG AACTTCTGAA GGACCCACTG TACATTGGTT TGAGGCATAA AAGAGTACGT 
GGACAGGCCT ACGATGAGCT TCTGGATGAA TTTATGAAGG CCATCACAGA CAGGTATGGG 
ATGAAATGCC TTATCCAGTT TGAAGATTTT GCCAACATTA ATGCCTTCCG CCTGTTGAGT 
AAATACCGCA ACAAATACCT CACATTCAAT GATGACATCC AAGGCACTGC TGCTGTGGCT 
GTGGCAGGCC TCCTAGCTGC CCTTCGCATT ACCAAAAGCA AAATGTGTGA CCATACCATT 
GTGTTTCAGG GTGCAGGCGA GGCTGCAATG GGCATAGGTG AGCTGATTGT CATGGCAATG 
GAGAAAGAGG GTCTTCCTCG TGAGCAGTGT ATAAAGAAAA TCTGGATGGT AGACTCTAAA 
GGACTTATAG TTAAGGGTCG AGATCACCTC ACCCATGAGA AAGAGAGGTT TGCACATGAG 
CATGCACAAA TGAAAAAGCT TGAGGATGTT GTAAAGGAGC TGAAACCCAC AGCTATAATT 
GGGGTGGCAG CAATCGCCGG CGCTTTCACA GAAGAGATAA TCAAGGCTAT GGCATCCTTC 
AATAAGAATC CTATTATCTT CGCTCTCAGC AACCCCACCA GCAAAGCTGA GTGTACAGCT 
GAACAGTGCT ACACACTCAC TGAGGGAAGG GGGATATTTG CAAGCGGAAG TCCTTTTGAT 
CCAGTAACTC TTCCCGATGG GAGGAAATTC TACCCTGGTC AGGGAAACAA CTCGTATGTG 
TTCCCTGGAG TGGGGCTGGG AGTCACTGCT TGTGAAATGA GACACATCCC TGAGGATGTT 
TTCCTTGTTA CAGCTGAGGC TATAGCAGAA CTGGTGACTG ACAAGGATCT CGCAGAGGGT 
CGTCTGTATC CTCCTCTCAG CAGCATTCGG GAAGTTTCCA TCAAACTGGC TGTGAAGATA 
GTGGAGTATG CATATAAGCA CAAAATGGCT ACGCTGCACC CAGAGCCGCA AGATAAAGAT 
GCTTTCATCC GTTCACTCAT CTATAGCACA GACTACCAAG AGTTTGCTGT GGACTCATAC 
AGCTGGCCTG AGAACGCCAT GACCGTCCAA ACATGTAAAC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

me1 ( XM_005158369.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158426.1 Danio rerio malic enzyme 1, NADP(+)-dependent, cytosolic (me1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa21666
Clone ID Related Accession (Same CDS sequence)	XM_005158369.4 , XM_017351366.1 , XM_005158369.3 , XM_017351366.2
Accession Version	XM_005158369.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	NADP-dependent malic enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158369.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTCT CCCTGGAGGA GCGCTTGCAG CTGGGCATTC ATGGGCTGTT GCCACCCTGC 
TTCCTCAGCC AGGATGTGCA AGTTTTGCGG GTCCTTAGGA GCTACGAGCT GTTAAAGGAA 
GATTTGGACA GGTATGTCTT TCTAATGGGC CTTCAGGACC GTAACGAGAA GCTGTTCTAC 
CGGGTGCTGA CCTCAGACAT AGAGAGATTC ATGCCAATTG TGTACACACC TACCGTTGGC 
CTAGCGTGCC AACAGTATGG CCTGACCTTC AGGAGACCGA GGGGCCTTTT TATTACAATT 
CATGACCGCT CCCACATCCC CACACTTCTT CAGAGCTGGC CGGAGAAGGA CATTCGTGCT 
GTAGTGGTGA CAGATGGAGA GAGGATTCTG GGCCTGGGGG ATCTGGGCTG CTATGGCATG 
GGCATCCCTG TGGGTAAACT GGCCCTGTAC ACTGCCTGTG GAGGAATGCC GCCTCAGCAG 
TGCCTGCCTG TTATGCTGGA CGTCGGCACA GACAAAGAGG AACTTCTGAA GGACCCACTG 
TACATTGGTT TGAGGCATAA AAGAGTACGT GGACAGGCCT ACGATGAGCT TCTGGATGAA 
TTTATGAAGG CCATCACAGA CAGGTATGGG ATGAAATGCC TTATCCAGTT TGAAGATTTT 
GCCAACATTA ATGCCTTCCG CCTGTTGAGT AAATACCGCA ACAAATACCT CACATTCAAT 
GATGACATCC AAGGCACTGC TGCTGTGGCT GTGGCAGGCC TCCTAGCTGC CCTTCGCATT 
ACCAAAAGCA AAATGTGTGA CCATACCATT GTGTTTCAGG GTGCAGGCGA GGCTGCAATG 
GGCATAGGTG AGCTGATTGT CATGGCAATG GAGAAAGAGG GTCTTCCTCG TGAGCAGTGT 
ATAAAGAAAA TCTGGATGGT AGACTCTAAA GGACTTATAG TTAAGGGTCG AGATCACCTC 
ACCCATGAGA AAGAGAGGTT TGCACATGAG CATGCACAAA TGAAAAAGCT TGAGGATGTT 
GTAAAGGAGC TGAAACCCAC AGCTATAATT GGGGTGGCAG CAATCGCCGG CGCTTTCACA 
GAAGAGATAA TCAAGGCTAT GGCATCCTTC AATAAGAATC CTATTATCTT CGCTCTCAGC 
AACCCCACCA GCAAAGCTGA GTGTACAGCT GAACAGTGCT ACACACTCAC TGAGGGAAGG 
GGGATATTTG CAAGCGGAAG TCCTTTTGAT CCAGTAACTC TTCCCGATGG GAGGAAATTC 
TACCCTGGTC AGGGAAACAA CTCGTATGTG TTCCCTGGAG TGGGGCTGGG AGTCACTGCT 
TGTGAAATGA GACACATCCC TGAGGATGTT TTCCTTGTTA CAGCTGAGGC TATAGCAGAA 
CTGGTGACTG ACAAGGATCT CGCAGAGGGT CGTCTGTATC CTCCTCTCAG CAGCATTCGG 
GAAGTTTCCA TCAAACTGGC TGTGAAGATA GTGGAGTATG CATATAAGCA CAAAATGGCT 
ACGCTGCACC CAGAGCCGCA AGATAAAGAT GCTTTCATCC GTTCACTCAT CTATAGCACA 
GACTACCAAG AGTTTGCTGT GGACTCATAC AGCTGGCCTG AGAACGCCAT GACCGTCCAA 
ACATGTAAAC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158426.1
CDS	97..1731
Translation	MAFSLEERLQLGIHGLLPPCFLSQDVQVLRVLRSYELLKEDLDRYVFLMGLQDRNEKLFYRVLTSDIERFMPIVYTPTVGLACQQYGLTFRRPRGLFITIHDRSHIPTLLQSWPEKDIRAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGMPPQQCLPVMLDVGTDKEELLKDPLYIGLRHKRVRGQAYDELLDEFMKAITDRYGMKCLIQFEDFANINAFRLLSKYRNKYLTFNDDIQGTAAVAVAGLLAALRITKSKMCDHTIVFQGAGEAAMGIGELIVMAMEKEGLPREQCIKKIWMVDSKGLIVKGRDHLTHEKERFAHEHAQMKKLEDVVKELKPTAIIGVAAIAGAFTEEIIKAMASFNKNPIIFALSNPTSKAECTAEQCYTLTEGRGIFASGSPFDPVTLPDGRKFYPGQGNNSYVFPGVGLGVTACEMRHIPEDVFLVTAEAIAELVTDKDLAEGRLYPPLSSIREVSIKLAVKIVEYAYKHKMATLHPEPQDKDAFIRSLIYSTDYQEFAVDSYSWPENAMTVQTCKL

Target ORF information:

RefSeq Version	XM_005158369.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio malic enzyme 1, NADP(+)-dependent, cytosolic (me1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158369.4

ORF Insert Sequence:

ATGGCCTTCT CCCTGGAGGA GCGCTTGCAG CTGGGCATTC ATGGGCTGTT GCCACCCTGC 
TTCCTCAGCC AGGATGTGCA AGTTTTGCGG GTCCTTAGGA GCTACGAGCT GTTAAAGGAA 
GATTTGGACA GGTATGTCTT TCTAATGGGC CTTCAGGACC GTAACGAGAA GCTGTTCTAC 
CGGGTGCTGA CCTCAGACAT AGAGAGATTC ATGCCAATTG TGTACACACC TACCGTTGGC 
CTAGCGTGCC AACAGTATGG CCTGACCTTC AGGAGACCGA GGGGCCTTTT TATTACAATT 
CATGACCGCT CCCACATCCC CACACTTCTT CAGAGCTGGC CGGAGAAGGA CATTCGTGCT 
GTAGTGGTGA CAGATGGAGA GAGGATTCTG GGCCTGGGGG ATCTGGGCTG CTATGGCATG 
GGCATCCCTG TGGGTAAACT GGCCCTGTAC ACTGCCTGTG GAGGAATGCC GCCTCAGCAG 
TGCCTGCCTG TTATGCTGGA CGTCGGCACA GACAAAGAGG AACTTCTGAA GGACCCACTG 
TACATTGGTT TGAGGCATAA AAGAGTACGT GGACAGGCCT ACGATGAGCT TCTGGATGAA 
TTTATGAAGG CCATCACAGA CAGGTATGGG ATGAAATGCC TTATCCAGTT TGAAGATTTT 
GCCAACATTA ATGCCTTCCG CCTGTTGAGT AAATACCGCA ACAAATACCT CACATTCAAT 
GATGACATCC AAGGCACTGC TGCTGTGGCT GTGGCAGGCC TCCTAGCTGC CCTTCGCATT 
ACCAAAAGCA AAATGTGTGA CCATACCATT GTGTTTCAGG GTGCAGGCGA GGCTGCAATG 
GGCATAGGTG AGCTGATTGT CATGGCAATG GAGAAAGAGG GTCTTCCTCG TGAGCAGTGT 
ATAAAGAAAA TCTGGATGGT AGACTCTAAA GGACTTATAG TTAAGGGTCG AGATCACCTC 
ACCCATGAGA AAGAGAGGTT TGCACATGAG CATGCACAAA TGAAAAAGCT TGAGGATGTT 
GTAAAGGAGC TGAAACCCAC AGCTATAATT GGGGTGGCAG CAATCGCCGG CGCTTTCACA 
GAAGAGATAA TCAAGGCTAT GGCATCCTTC AATAAGAATC CTATTATCTT CGCTCTCAGC 
AACCCCACCA GCAAAGCTGA GTGTACAGCT GAACAGTGCT ACACACTCAC TGAGGGAAGG 
GGGATATTTG CAAGCGGAAG TCCTTTTGAT CCAGTAACTC TTCCCGATGG GAGGAAATTC 
TACCCTGGTC AGGGAAACAA CTCGTATGTG TTCCCTGGAG TGGGGCTGGG AGTCACTGCT 
TGTGAAATGA GACACATCCC TGAGGATGTT TTCCTTGTTA CAGCTGAGGC TATAGCAGAA 
CTGGTGACTG ACAAGGATCT CGCAGAGGGT CGTCTGTATC CTCCTCTCAG CAGCATTCGG 
GAAGTTTCCA TCAAACTGGC TGTGAAGATA GTGGAGTATG CATATAAGCA CAAAATGGCT 
ACGCTGCACC CAGAGCCGCA AGATAAAGAT GCTTTCATCC GTTCACTCAT CTATAGCACA 
GACTACCAAG AGTTTGCTGT GGACTCATAC AGCTGGCCTG AGAACGCCAT GACCGTCCAA 
ACATGTAAAC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

me1 ( XM_017351366.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206855.1 Danio rerio malic enzyme 1, NADP(+)-dependent, cytosolic (me1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa21666
Clone ID Related Accession (Same CDS sequence)	XM_005158369.4 , XM_017351366.1 , XM_005158369.3 , XM_017351366.2
Accession Version	XM_017351366.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	NADP-dependent malic enzyme isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTCT CCCTGGAGGA GCGCTTGCAG CTGGGCATTC ATGGGCTGTT GCCACCCTGC 
TTCCTCAGCC AGGATGTGCA AGTTTTGCGG GTCCTTAGGA GCTACGAGCT GTTAAAGGAA 
GATTTGGACA GGTATGTCTT TCTAATGGGC CTTCAGGACC GTAACGAGAA GCTGTTCTAC 
CGGGTGCTGA CCTCAGACAT AGAGAGATTC ATGCCAATTG TGTACACACC TACCGTTGGC 
CTAGCGTGCC AACAGTATGG CCTGACCTTC AGGAGACCGA GGGGCCTTTT TATTACAATT 
CATGACCGCT CCCACATCCC CACACTTCTT CAGAGCTGGC CGGAGAAGGA CATTCGTGCT 
GTAGTGGTGA CAGATGGAGA GAGGATTCTG GGCCTGGGGG ATCTGGGCTG CTATGGCATG 
GGCATCCCTG TGGGTAAACT GGCCCTGTAC ACTGCCTGTG GAGGAATGCC GCCTCAGCAG 
TGCCTGCCTG TTATGCTGGA CGTCGGCACA GACAAAGAGG AACTTCTGAA GGACCCACTG 
TACATTGGTT TGAGGCATAA AAGAGTACGT GGACAGGCCT ACGATGAGCT TCTGGATGAA 
TTTATGAAGG CCATCACAGA CAGGTATGGG ATGAAATGCC TTATCCAGTT TGAAGATTTT 
GCCAACATTA ATGCCTTCCG CCTGTTGAGT AAATACCGCA ACAAATACCT CACATTCAAT 
GATGACATCC AAGGCACTGC TGCTGTGGCT GTGGCAGGCC TCCTAGCTGC CCTTCGCATT 
ACCAAAAGCA AAATGTGTGA CCATACCATT GTGTTTCAGG GTGCAGGCGA GGCTGCAATG 
GGCATAGGTG AGCTGATTGT CATGGCAATG GAGAAAGAGG GTCTTCCTCG TGAGCAGTGT 
ATAAAGAAAA TCTGGATGGT AGACTCTAAA GGACTTATAG TTAAGGGTCG AGATCACCTC 
ACCCATGAGA AAGAGAGGTT TGCACATGAG CATGCACAAA TGAAAAAGCT TGAGGATGTT 
GTAAAGGAGC TGAAACCCAC AGCTATAATT GGGGTGGCAG CAATCGCCGG CGCTTTCACA 
GAAGAGATAA TCAAGGCTAT GGCATCCTTC AATAAGAATC CTATTATCTT CGCTCTCAGC 
AACCCCACCA GCAAAGCTGA GTGTACAGCT GAACAGTGCT ACACACTCAC TGAGGGAAGG 
GGGATATTTG CAAGCGGAAG TCCTTTTGAT CCAGTAACTC TTCCCGATGG GAGGAAATTC 
TACCCTGGTC AGGGAAACAA CTCGTATGTG TTCCCTGGAG TGGGGCTGGG AGTCACTGCT 
TGTGAAATGA GACACATCCC TGAGGATGTT TTCCTTGTTA CAGCTGAGGC TATAGCAGAA 
CTGGTGACTG ACAAGGATCT CGCAGAGGGT CGTCTGTATC CTCCTCTCAG CAGCATTCGG 
GAAGTTTCCA TCAAACTGGC TGTGAAGATA GTGGAGTATG CATATAAGCA CAAAATGGCT 
ACGCTGCACC CAGAGCCGCA AGATAAAGAT GCTTTCATCC GTTCACTCAT CTATAGCACA 
GACTACCAAG AGTTTGCTGT GGACTCATAC AGCTGGCCTG AGAACGCCAT GACCGTCCAA 
ACATGTAAAC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206855.1
CDS	95..1729
Translation	MAFSLEERLQLGIHGLLPPCFLSQDVQVLRVLRSYELLKEDLDRYVFLMGLQDRNEKLFYRVLTSDIERFMPIVYTPTVGLACQQYGLTFRRPRGLFITIHDRSHIPTLLQSWPEKDIRAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGMPPQQCLPVMLDVGTDKEELLKDPLYIGLRHKRVRGQAYDELLDEFMKAITDRYGMKCLIQFEDFANINAFRLLSKYRNKYLTFNDDIQGTAAVAVAGLLAALRITKSKMCDHTIVFQGAGEAAMGIGELIVMAMEKEGLPREQCIKKIWMVDSKGLIVKGRDHLTHEKERFAHEHAQMKKLEDVVKELKPTAIIGVAAIAGAFTEEIIKAMASFNKNPIIFALSNPTSKAECTAEQCYTLTEGRGIFASGSPFDPVTLPDGRKFYPGQGNNSYVFPGVGLGVTACEMRHIPEDVFLVTAEAIAELVTDKDLAEGRLYPPLSSIREVSIKLAVKIVEYAYKHKMATLHPEPQDKDAFIRSLIYSTDYQEFAVDSYSWPENAMTVQTCKL

Target ORF information:

RefSeq Version	XM_017351366.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio malic enzyme 1, NADP(+)-dependent, cytosolic (me1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351366.1

ORF Insert Sequence:

ATGGCCTTCT CCCTGGAGGA GCGCTTGCAG CTGGGCATTC ATGGGCTGTT GCCACCCTGC 
TTCCTCAGCC AGGATGTGCA AGTTTTGCGG GTCCTTAGGA GCTACGAGCT GTTAAAGGAA 
GATTTGGACA GGTATGTCTT TCTAATGGGC CTTCAGGACC GTAACGAGAA GCTGTTCTAC 
CGGGTGCTGA CCTCAGACAT AGAGAGATTC ATGCCAATTG TGTACACACC TACCGTTGGC 
CTAGCGTGCC AACAGTATGG CCTGACCTTC AGGAGACCGA GGGGCCTTTT TATTACAATT 
CATGACCGCT CCCACATCCC CACACTTCTT CAGAGCTGGC CGGAGAAGGA CATTCGTGCT 
GTAGTGGTGA CAGATGGAGA GAGGATTCTG GGCCTGGGGG ATCTGGGCTG CTATGGCATG 
GGCATCCCTG TGGGTAAACT GGCCCTGTAC ACTGCCTGTG GAGGAATGCC GCCTCAGCAG 
TGCCTGCCTG TTATGCTGGA CGTCGGCACA GACAAAGAGG AACTTCTGAA GGACCCACTG 
TACATTGGTT TGAGGCATAA AAGAGTACGT GGACAGGCCT ACGATGAGCT TCTGGATGAA 
TTTATGAAGG CCATCACAGA CAGGTATGGG ATGAAATGCC TTATCCAGTT TGAAGATTTT 
GCCAACATTA ATGCCTTCCG CCTGTTGAGT AAATACCGCA ACAAATACCT CACATTCAAT 
GATGACATCC AAGGCACTGC TGCTGTGGCT GTGGCAGGCC TCCTAGCTGC CCTTCGCATT 
ACCAAAAGCA AAATGTGTGA CCATACCATT GTGTTTCAGG GTGCAGGCGA GGCTGCAATG 
GGCATAGGTG AGCTGATTGT CATGGCAATG GAGAAAGAGG GTCTTCCTCG TGAGCAGTGT 
ATAAAGAAAA TCTGGATGGT AGACTCTAAA GGACTTATAG TTAAGGGTCG AGATCACCTC 
ACCCATGAGA AAGAGAGGTT TGCACATGAG CATGCACAAA TGAAAAAGCT TGAGGATGTT 
GTAAAGGAGC TGAAACCCAC AGCTATAATT GGGGTGGCAG CAATCGCCGG CGCTTTCACA 
GAAGAGATAA TCAAGGCTAT GGCATCCTTC AATAAGAATC CTATTATCTT CGCTCTCAGC 
AACCCCACCA GCAAAGCTGA GTGTACAGCT GAACAGTGCT ACACACTCAC TGAGGGAAGG 
GGGATATTTG CAAGCGGAAG TCCTTTTGAT CCAGTAACTC TTCCCGATGG GAGGAAATTC 
TACCCTGGTC AGGGAAACAA CTCGTATGTG TTCCCTGGAG TGGGGCTGGG AGTCACTGCT 
TGTGAAATGA GACACATCCC TGAGGATGTT TTCCTTGTTA CAGCTGAGGC TATAGCAGAA 
CTGGTGACTG ACAAGGATCT CGCAGAGGGT CGTCTGTATC CTCCTCTCAG CAGCATTCGG 
GAAGTTTCCA TCAAACTGGC TGTGAAGATA GTGGAGTATG CATATAAGCA CAAAATGGCT 
ACGCTGCACC CAGAGCCGCA AGATAAAGAT GCTTTCATCC GTTCACTCAT CTATAGCACA 
GACTACCAAG AGTTTGCTGT GGACTCATAC AGCTGGCCTG AGAACGCCAT GACCGTCCAA 
ACATGTAAAC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

me1 ( XM_005158369.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158426.1 Danio rerio malic enzyme 1, NADP(+)-dependent, cytosolic (me1), transcript variant X1, mRNA.

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CloneID	ODa21666
Clone ID Related Accession (Same CDS sequence)	XM_005158369.4 , XM_017351366.1 , XM_005158369.3 , XM_017351366.2
Accession Version	XM_005158369.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	NADP-dependent malic enzyme isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158369.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTCT CCCTGGAGGA GCGCTTGCAG CTGGGCATTC ATGGGCTGTT GCCACCCTGC 
TTCCTCAGCC AGGATGTGCA AGTTTTGCGG GTCCTTAGGA GCTACGAGCT GTTAAAGGAA 
GATTTGGACA GGTATGTCTT TCTAATGGGC CTTCAGGACC GTAACGAGAA GCTGTTCTAC 
CGGGTGCTGA CCTCAGACAT AGAGAGATTC ATGCCAATTG TGTACACACC TACCGTTGGC 
CTAGCGTGCC AACAGTATGG CCTGACCTTC AGGAGACCGA GGGGCCTTTT TATTACAATT 
CATGACCGCT CCCACATCCC CACACTTCTT CAGAGCTGGC CGGAGAAGGA CATTCGTGCT 
GTAGTGGTGA CAGATGGAGA GAGGATTCTG GGCCTGGGGG ATCTGGGCTG CTATGGCATG 
GGCATCCCTG TGGGTAAACT GGCCCTGTAC ACTGCCTGTG GAGGAATGCC GCCTCAGCAG 
TGCCTGCCTG TTATGCTGGA CGTCGGCACA GACAAAGAGG AACTTCTGAA GGACCCACTG 
TACATTGGTT TGAGGCATAA AAGAGTACGT GGACAGGCCT ACGATGAGCT TCTGGATGAA 
TTTATGAAGG CCATCACAGA CAGGTATGGG ATGAAATGCC TTATCCAGTT TGAAGATTTT 
GCCAACATTA ATGCCTTCCG CCTGTTGAGT AAATACCGCA ACAAATACCT CACATTCAAT 
GATGACATCC AAGGCACTGC TGCTGTGGCT GTGGCAGGCC TCCTAGCTGC CCTTCGCATT 
ACCAAAAGCA AAATGTGTGA CCATACCATT GTGTTTCAGG GTGCAGGCGA GGCTGCAATG 
GGCATAGGTG AGCTGATTGT CATGGCAATG GAGAAAGAGG GTCTTCCTCG TGAGCAGTGT 
ATAAAGAAAA TCTGGATGGT AGACTCTAAA GGACTTATAG TTAAGGGTCG AGATCACCTC 
ACCCATGAGA AAGAGAGGTT TGCACATGAG CATGCACAAA TGAAAAAGCT TGAGGATGTT 
GTAAAGGAGC TGAAACCCAC AGCTATAATT GGGGTGGCAG CAATCGCCGG CGCTTTCACA 
GAAGAGATAA TCAAGGCTAT GGCATCCTTC AATAAGAATC CTATTATCTT CGCTCTCAGC 
AACCCCACCA GCAAAGCTGA GTGTACAGCT GAACAGTGCT ACACACTCAC TGAGGGAAGG 
GGGATATTTG CAAGCGGAAG TCCTTTTGAT CCAGTAACTC TTCCCGATGG GAGGAAATTC 
TACCCTGGTC AGGGAAACAA CTCGTATGTG TTCCCTGGAG TGGGGCTGGG AGTCACTGCT 
TGTGAAATGA GACACATCCC TGAGGATGTT TTCCTTGTTA CAGCTGAGGC TATAGCAGAA 
CTGGTGACTG ACAAGGATCT CGCAGAGGGT CGTCTGTATC CTCCTCTCAG CAGCATTCGG 
GAAGTTTCCA TCAAACTGGC TGTGAAGATA GTGGAGTATG CATATAAGCA CAAAATGGCT 
ACGCTGCACC CAGAGCCGCA AGATAAAGAT GCTTTCATCC GTTCACTCAT CTATAGCACA 
GACTACCAAG AGTTTGCTGT GGACTCATAC AGCTGGCCTG AGAACGCCAT GACCGTCCAA 
ACATGTAAAC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158426.1
CDS	59..1693
Translation	MAFSLEERLQLGIHGLLPPCFLSQDVQVLRVLRSYELLKEDLDRYVFLMGLQDRNEKLFYRVLTSDIERFMPIVYTPTVGLACQQYGLTFRRPRGLFITIHDRSHIPTLLQSWPEKDIRAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGMPPQQCLPVMLDVGTDKEELLKDPLYIGLRHKRVRGQAYDELLDEFMKAITDRYGMKCLIQFEDFANINAFRLLSKYRNKYLTFNDDIQGTAAVAVAGLLAALRITKSKMCDHTIVFQGAGEAAMGIGELIVMAMEKEGLPREQCIKKIWMVDSKGLIVKGRDHLTHEKERFAHEHAQMKKLEDVVKELKPTAIIGVAAIAGAFTEEIIKAMASFNKNPIIFALSNPTSKAECTAEQCYTLTEGRGIFASGSPFDPVTLPDGRKFYPGQGNNSYVFPGVGLGVTACEMRHIPEDVFLVTAEAIAELVTDKDLAEGRLYPPLSSIREVSIKLAVKIVEYAYKHKMATLHPEPQDKDAFIRSLIYSTDYQEFAVDSYSWPENAMTVQTCKL

Target ORF information:

RefSeq Version	XM_005158369.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio malic enzyme 1, NADP(+)-dependent, cytosolic (me1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158369.3

ORF Insert Sequence:

ATGGCCTTCT CCCTGGAGGA GCGCTTGCAG CTGGGCATTC ATGGGCTGTT GCCACCCTGC 
TTCCTCAGCC AGGATGTGCA AGTTTTGCGG GTCCTTAGGA GCTACGAGCT GTTAAAGGAA 
GATTTGGACA GGTATGTCTT TCTAATGGGC CTTCAGGACC GTAACGAGAA GCTGTTCTAC 
CGGGTGCTGA CCTCAGACAT AGAGAGATTC ATGCCAATTG TGTACACACC TACCGTTGGC 
CTAGCGTGCC AACAGTATGG CCTGACCTTC AGGAGACCGA GGGGCCTTTT TATTACAATT 
CATGACCGCT CCCACATCCC CACACTTCTT CAGAGCTGGC CGGAGAAGGA CATTCGTGCT 
GTAGTGGTGA CAGATGGAGA GAGGATTCTG GGCCTGGGGG ATCTGGGCTG CTATGGCATG 
GGCATCCCTG TGGGTAAACT GGCCCTGTAC ACTGCCTGTG GAGGAATGCC GCCTCAGCAG 
TGCCTGCCTG TTATGCTGGA CGTCGGCACA GACAAAGAGG AACTTCTGAA GGACCCACTG 
TACATTGGTT TGAGGCATAA AAGAGTACGT GGACAGGCCT ACGATGAGCT TCTGGATGAA 
TTTATGAAGG CCATCACAGA CAGGTATGGG ATGAAATGCC TTATCCAGTT TGAAGATTTT 
GCCAACATTA ATGCCTTCCG CCTGTTGAGT AAATACCGCA ACAAATACCT CACATTCAAT 
GATGACATCC AAGGCACTGC TGCTGTGGCT GTGGCAGGCC TCCTAGCTGC CCTTCGCATT 
ACCAAAAGCA AAATGTGTGA CCATACCATT GTGTTTCAGG GTGCAGGCGA GGCTGCAATG 
GGCATAGGTG AGCTGATTGT CATGGCAATG GAGAAAGAGG GTCTTCCTCG TGAGCAGTGT 
ATAAAGAAAA TCTGGATGGT AGACTCTAAA GGACTTATAG TTAAGGGTCG AGATCACCTC 
ACCCATGAGA AAGAGAGGTT TGCACATGAG CATGCACAAA TGAAAAAGCT TGAGGATGTT 
GTAAAGGAGC TGAAACCCAC AGCTATAATT GGGGTGGCAG CAATCGCCGG CGCTTTCACA 
GAAGAGATAA TCAAGGCTAT GGCATCCTTC AATAAGAATC CTATTATCTT CGCTCTCAGC 
AACCCCACCA GCAAAGCTGA GTGTACAGCT GAACAGTGCT ACACACTCAC TGAGGGAAGG 
GGGATATTTG CAAGCGGAAG TCCTTTTGAT CCAGTAACTC TTCCCGATGG GAGGAAATTC 
TACCCTGGTC AGGGAAACAA CTCGTATGTG TTCCCTGGAG TGGGGCTGGG AGTCACTGCT 
TGTGAAATGA GACACATCCC TGAGGATGTT TTCCTTGTTA CAGCTGAGGC TATAGCAGAA 
CTGGTGACTG ACAAGGATCT CGCAGAGGGT CGTCTGTATC CTCCTCTCAG CAGCATTCGG 
GAAGTTTCCA TCAAACTGGC TGTGAAGATA GTGGAGTATG CATATAAGCA CAAAATGGCT 
ACGCTGCACC CAGAGCCGCA AGATAAAGAT GCTTTCATCC GTTCACTCAT CTATAGCACA 
GACTACCAAG AGTTTGCTGT GGACTCATAC AGCTGGCCTG AGAACGCCAT GACCGTCCAA 
ACATGTAAAC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

me1 ( XM_017351366.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206855.1 Danio rerio malic enzyme 1, NADP(+)-dependent, cytosolic (me1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa21666
Clone ID Related Accession (Same CDS sequence)	XM_005158369.4 , XM_017351366.1 , XM_005158369.3 , XM_017351366.2
Accession Version	XM_017351366.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	NADP-dependent malic enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351366.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTCT CCCTGGAGGA GCGCTTGCAG CTGGGCATTC ATGGGCTGTT GCCACCCTGC 
TTCCTCAGCC AGGATGTGCA AGTTTTGCGG GTCCTTAGGA GCTACGAGCT GTTAAAGGAA 
GATTTGGACA GGTATGTCTT TCTAATGGGC CTTCAGGACC GTAACGAGAA GCTGTTCTAC 
CGGGTGCTGA CCTCAGACAT AGAGAGATTC ATGCCAATTG TGTACACACC TACCGTTGGC 
CTAGCGTGCC AACAGTATGG CCTGACCTTC AGGAGACCGA GGGGCCTTTT TATTACAATT 
CATGACCGCT CCCACATCCC CACACTTCTT CAGAGCTGGC CGGAGAAGGA CATTCGTGCT 
GTAGTGGTGA CAGATGGAGA GAGGATTCTG GGCCTGGGGG ATCTGGGCTG CTATGGCATG 
GGCATCCCTG TGGGTAAACT GGCCCTGTAC ACTGCCTGTG GAGGAATGCC GCCTCAGCAG 
TGCCTGCCTG TTATGCTGGA CGTCGGCACA GACAAAGAGG AACTTCTGAA GGACCCACTG 
TACATTGGTT TGAGGCATAA AAGAGTACGT GGACAGGCCT ACGATGAGCT TCTGGATGAA 
TTTATGAAGG CCATCACAGA CAGGTATGGG ATGAAATGCC TTATCCAGTT TGAAGATTTT 
GCCAACATTA ATGCCTTCCG CCTGTTGAGT AAATACCGCA ACAAATACCT CACATTCAAT 
GATGACATCC AAGGCACTGC TGCTGTGGCT GTGGCAGGCC TCCTAGCTGC CCTTCGCATT 
ACCAAAAGCA AAATGTGTGA CCATACCATT GTGTTTCAGG GTGCAGGCGA GGCTGCAATG 
GGCATAGGTG AGCTGATTGT CATGGCAATG GAGAAAGAGG GTCTTCCTCG TGAGCAGTGT 
ATAAAGAAAA TCTGGATGGT AGACTCTAAA GGACTTATAG TTAAGGGTCG AGATCACCTC 
ACCCATGAGA AAGAGAGGTT TGCACATGAG CATGCACAAA TGAAAAAGCT TGAGGATGTT 
GTAAAGGAGC TGAAACCCAC AGCTATAATT GGGGTGGCAG CAATCGCCGG CGCTTTCACA 
GAAGAGATAA TCAAGGCTAT GGCATCCTTC AATAAGAATC CTATTATCTT CGCTCTCAGC 
AACCCCACCA GCAAAGCTGA GTGTACAGCT GAACAGTGCT ACACACTCAC TGAGGGAAGG 
GGGATATTTG CAAGCGGAAG TCCTTTTGAT CCAGTAACTC TTCCCGATGG GAGGAAATTC 
TACCCTGGTC AGGGAAACAA CTCGTATGTG TTCCCTGGAG TGGGGCTGGG AGTCACTGCT 
TGTGAAATGA GACACATCCC TGAGGATGTT TTCCTTGTTA CAGCTGAGGC TATAGCAGAA 
CTGGTGACTG ACAAGGATCT CGCAGAGGGT CGTCTGTATC CTCCTCTCAG CAGCATTCGG 
GAAGTTTCCA TCAAACTGGC TGTGAAGATA GTGGAGTATG CATATAAGCA CAAAATGGCT 
ACGCTGCACC CAGAGCCGCA AGATAAAGAT GCTTTCATCC GTTCACTCAT CTATAGCACA 
GACTACCAAG AGTTTGCTGT GGACTCATAC AGCTGGCCTG AGAACGCCAT GACCGTCCAA 
ACATGTAAAC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206855.1
CDS	127..1761
Translation	MAFSLEERLQLGIHGLLPPCFLSQDVQVLRVLRSYELLKEDLDRYVFLMGLQDRNEKLFYRVLTSDIERFMPIVYTPTVGLACQQYGLTFRRPRGLFITIHDRSHIPTLLQSWPEKDIRAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGMPPQQCLPVMLDVGTDKEELLKDPLYIGLRHKRVRGQAYDELLDEFMKAITDRYGMKCLIQFEDFANINAFRLLSKYRNKYLTFNDDIQGTAAVAVAGLLAALRITKSKMCDHTIVFQGAGEAAMGIGELIVMAMEKEGLPREQCIKKIWMVDSKGLIVKGRDHLTHEKERFAHEHAQMKKLEDVVKELKPTAIIGVAAIAGAFTEEIIKAMASFNKNPIIFALSNPTSKAECTAEQCYTLTEGRGIFASGSPFDPVTLPDGRKFYPGQGNNSYVFPGVGLGVTACEMRHIPEDVFLVTAEAIAELVTDKDLAEGRLYPPLSSIREVSIKLAVKIVEYAYKHKMATLHPEPQDKDAFIRSLIYSTDYQEFAVDSYSWPENAMTVQTCKL

Target ORF information:

RefSeq Version	XM_017351366.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio malic enzyme 1, NADP(+)-dependent, cytosolic (me1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351366.2

ORF Insert Sequence:

ATGGCCTTCT CCCTGGAGGA GCGCTTGCAG CTGGGCATTC ATGGGCTGTT GCCACCCTGC 
TTCCTCAGCC AGGATGTGCA AGTTTTGCGG GTCCTTAGGA GCTACGAGCT GTTAAAGGAA 
GATTTGGACA GGTATGTCTT TCTAATGGGC CTTCAGGACC GTAACGAGAA GCTGTTCTAC 
CGGGTGCTGA CCTCAGACAT AGAGAGATTC ATGCCAATTG TGTACACACC TACCGTTGGC 
CTAGCGTGCC AACAGTATGG CCTGACCTTC AGGAGACCGA GGGGCCTTTT TATTACAATT 
CATGACCGCT CCCACATCCC CACACTTCTT CAGAGCTGGC CGGAGAAGGA CATTCGTGCT 
GTAGTGGTGA CAGATGGAGA GAGGATTCTG GGCCTGGGGG ATCTGGGCTG CTATGGCATG 
GGCATCCCTG TGGGTAAACT GGCCCTGTAC ACTGCCTGTG GAGGAATGCC GCCTCAGCAG 
TGCCTGCCTG TTATGCTGGA CGTCGGCACA GACAAAGAGG AACTTCTGAA GGACCCACTG 
TACATTGGTT TGAGGCATAA AAGAGTACGT GGACAGGCCT ACGATGAGCT TCTGGATGAA 
TTTATGAAGG CCATCACAGA CAGGTATGGG ATGAAATGCC TTATCCAGTT TGAAGATTTT 
GCCAACATTA ATGCCTTCCG CCTGTTGAGT AAATACCGCA ACAAATACCT CACATTCAAT 
GATGACATCC AAGGCACTGC TGCTGTGGCT GTGGCAGGCC TCCTAGCTGC CCTTCGCATT 
ACCAAAAGCA AAATGTGTGA CCATACCATT GTGTTTCAGG GTGCAGGCGA GGCTGCAATG 
GGCATAGGTG AGCTGATTGT CATGGCAATG GAGAAAGAGG GTCTTCCTCG TGAGCAGTGT 
ATAAAGAAAA TCTGGATGGT AGACTCTAAA GGACTTATAG TTAAGGGTCG AGATCACCTC 
ACCCATGAGA AAGAGAGGTT TGCACATGAG CATGCACAAA TGAAAAAGCT TGAGGATGTT 
GTAAAGGAGC TGAAACCCAC AGCTATAATT GGGGTGGCAG CAATCGCCGG CGCTTTCACA 
GAAGAGATAA TCAAGGCTAT GGCATCCTTC AATAAGAATC CTATTATCTT CGCTCTCAGC 
AACCCCACCA GCAAAGCTGA GTGTACAGCT GAACAGTGCT ACACACTCAC TGAGGGAAGG 
GGGATATTTG CAAGCGGAAG TCCTTTTGAT CCAGTAACTC TTCCCGATGG GAGGAAATTC 
TACCCTGGTC AGGGAAACAA CTCGTATGTG TTCCCTGGAG TGGGGCTGGG AGTCACTGCT 
TGTGAAATGA GACACATCCC TGAGGATGTT TTCCTTGTTA CAGCTGAGGC TATAGCAGAA 
CTGGTGACTG ACAAGGATCT CGCAGAGGGT CGTCTGTATC CTCCTCTCAG CAGCATTCGG 
GAAGTTTCCA TCAAACTGGC TGTGAAGATA GTGGAGTATG CATATAAGCA CAAAATGGCT 
ACGCTGCACC CAGAGCCGCA AGATAAAGAT GCTTTCATCC GTTCACTCAT CTATAGCACA 
GACTACCAAG AGTTTGCTGT GGACTCATAC AGCTGGCCTG AGAACGCCAT GACCGTCCAA 
ACATGTAAAC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SREBP-1 and LXRα pathways mediated Cu-induced hepatic lipid metabolism in zebrafish Danio rerio.
Chemosphere215370-379(2019 Jan)
Pan YX,Zhuo MQ,Li DD,Xu YH,Wu K,Luo Z

Oxidative stress and mitochondrial dysfunction mediated Cd-induced hepatic lipid accumulation in zebrafish Danio rerio.
Aquatic toxicology (Amsterdam, Netherlands)19912-20(2018 Jun)
Pan YX,Luo Z,Zhuo MQ,Wei CC,Chen GH,Song YF

Transforming growth factor-β1 regulates the nascent hematopoietic stem cell niche by promoting gluconeogenesis.
Leukemia32(2)479-491(2018 02)
Zhang CY,Yin HM,Wang H,Su D,Xia Y,Yan LF,Fang B,Liu W,Wang YM,Gu AH,Zhou Y

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Spatiotemporal expression and transcriptional regulation of heme oxygenase and biliverdin reductase genes in zebrafish (Danio rerio) suggest novel roles during early developmental periods of heightened oxidative stress.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP191138-151(2017 Jan)
Holowiecki A,O'Shields B,Jenny MJ

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me1 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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