LLGL1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LLGL1
Entrez Gene ID	751760
Full Name	lethal giant larvae homolog 1 (Drosophila)
Synonyms	zgc:153337
General protein information	Preferred Names lethal giant larvae homolog 1 (Drosophila) Names lethal(2) giant larvae protein homolog 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005164141.3	XP_005164198.1	lethal(2) giant larvae protein homolog 1 isoform X1
XM_005164142.4	XP_005164199.1	lethal(2) giant larvae protein homolog 1 isoform X2
NM_001045469.2	NP_001038934.2	lethal(2) giant larvae protein homolog 1
XM_005164141.4	XP_005164198.1	lethal(2) giant larvae protein homolog 1 isoform X1
XM_021471471.1	XP_021327146.1	lethal(2) giant larvae protein homolog 1 isoform X3
XM_005164142.3	XP_005164199.1	lethal(2) giant larvae protein homolog 1 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LLGL1	XP_005620208.1
Mus musculus (house mouse)	Llgl1	NP_001152877.1
Rattus norvegicus (Norway rat)	Llgl1	NP_690057.1
Bos taurus (cattle)	LLGL1	NP_001073811.1
Danio rerio (zebrafish)	llgl1	NP_001038934.2
Drosophila melanogaster (fruit fly)	l(2)gl	NP_001259787.1
Xenopus tropicalis (tropical clawed frog)	llgl1	XP_004918125.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004483	XP_313781.5
Macaca mulatta (Rhesus monkey)	LLGL1	XP_001095816.1
Gallus gallus (chicken)	LLGL1	XP_003642193.2
Homo sapiens (human)	LLGL1	NP_004131.3
Pan troglodytes (chimpanzee)	LLGL1	XP_523574.3

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

aPKC regulates apical localization of Lgl to restrict elongation of microridges in developing zebrafish epidermis.
Raman R, Damle I, Rote R, Banerjee S, Dingare C, Sonawane M
Nature communications711643(2016 06)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Scube activity is necessary for Hedgehog signal transduction in vivo.
Johnson JL, Hall TE, Dyson JM, Sonntag C, Ayers K, Berger S, Gautier P, Mitchell C, Hollway GE, Currie PD
Developmental biology368(2)193-202(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LLGL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LLGL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35424	NM_001045469.2 Latest version!	Danio rerio LLGL scribble cell polarity complex component 1 (llgl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa08598	XM_005164141.4 Latest version!	Danio rerio lethal giant larvae homolog 1 (Drosophila) (llgl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa08599	XM_005164142.4 Latest version!	Danio rerio lethal giant larvae homolog 1 (Drosophila) (llgl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa141598	XM_021471471.1 Latest version!	Danio rerio lethal giant larvae homolog 1 (Drosophila) (llgl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa08598	XM_005164141.3	Danio rerio lethal giant larvae homolog 1 (Drosophila) (llgl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa08599	XM_005164142.3	Danio rerio lethal giant larvae homolog 1 (Drosophila) (llgl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LLGL1 ( NM_001045469.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001038934.2 Danio rerio LLGL scribble cell polarity complex component 1 (llgl1), mRNA.

Price & Availability↑

CloneID	ODa35424
Clone ID Related Accession (Same CDS sequence)	NM_001045469.2
Accession Version	NM_001045469.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554566400000
Organism	Danio rerio(zebrafish)
Product	lethal(2) giant larvae protein homolog 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JQ688301.1. On Apr 22, 2012 this sequence version replaced NM_001045469.1. ##Evidence-Data-START## Transcript exon combination :: JQ688301.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGATGAAGT TTAGGTTCAG ACGGCAGGGA AATGACCCTC ATCGTGAAAA AATCAAGCAG 
GATCTGTTTG CATTCAATAA GACAGTTGAA CATGGATTTC CAAACCAGCC AAGTGCTTTG 
GCCTATGATC CCAAACTGCA GCTTATGGCT ATTGGAACTA AATCAGGGGC CATCAAAATA 
TATGGTGCTC CAGGGGTAGA GATGACGGGA CTTCATAAAG ACACTGCTGC TGTTACGCAG 
ATACACTTCC TCTGTGGACA GGGAAGAATA CTTTCTCTTT TGGATGATAA CACGATTCAC 
CTTTGGGAGA TCGTGCAGAG AGAAAACCGA TCACATTTGC TAGAAGTTCA CAGCTTCAAC 
CTGCCTGGCA GGCCGGGCAT CGAGAGCACA AGTGCAACTC GTGTGACGGT CATGCTGCTG 
AATCTGAGCT GTAATCTTCT CGCTCTGGGG ACTGAAGGTG GTGGGGTTCA CTTTCTGGAG 
TTACCTACCC TCACACTGCT CAACAAGTCT CTGTTTCAGG ACGAGATCAT GCAGAGCGTT 
CCAGAGGAGT ACAAGTGTGG AAAATCATTG GGGCCTGTGG AGTCCCTGCA GGAACATCCG 
AAAGACTCAG ATAAAATCCT CATTGGCTAC AGCAGAGGTC TGGTGGTGCT CTGGGACCTC 
AGCAGTCGGC ATGTGGACAA CCTCTTCCTG GGAAAGCAGC AGCTGGAGAG TTTGGTCTGG 
GAACGGTCCG GGAACAGCTT TGTTAGTTCT CATAGTGATG GCGGATACAT GGTGTGGGCT 
GTCAGCAGCA GCAATCCCTG CACCCATGAC CCCGTCTCAT CCACTATACC ATATGGCCCC 
TTTCCTTGCA AAGCCGTGAA CAAGATCCTG TGGAGGACGA CAGAATCAGG AGCTCCTCTA 
GTGTTATTCA GTGGCGGGAT GCCCAGAGCC AGCTATGGAG ACAGACACTG CCTCACCATC 
CTGCAGGACA GCAGCCACGT CACGCTGGAC TTCACCTCGC GTGTCATCGA CTTCTTCACT 
ATTCACTGCA CGGATACAGA GAAGGACTTT GACGAGCCTT CAGCACTGGT GGTTCTTCTG 
GAGGAGGAGC TGGTGGTGAT TGATCTTCAG ATGCCTGGAT GGCCCACAGT CCCTGCTCCA 
TATCTGGCTC CTCTGCACTC TTCCGCCATC ACCTGCTCCT GCCACATCTC CAATGTGCCT 
CCCAAACTGT GGGAGCGAGT GATCAGTGCT GGCCAACAGC AGTGTCCGCT GCAGAACTAT 
GAGAATTGGC CAATATGTGG AGGGAAAAAC TTAGCGCCTG ATCCAAAACA GAAGGAACTG 
CTGTTAACTG GTCATGAGGA CGGAACAGTG CGTTTCTGGG AGGCGTCAGG AGTGTCACTC 
AAACCTTTAT ACAAACTGAG CACGGCCTCC ATCTTTCAGA CAGACTGCGA GCACAACGAC 
AGCCTGACAC AAGCCGGAGA GGAGGAATGG CCTCCATTCA GAAAGGTGGG CTGCTTTGAC 
CCATACAGTG ATGACCCCAG ACTTGGAATC CAGAAAATCA GCCTCTGTAA ATACAGTGGA 
AAACTGGTGG TGGCTGGAAC TGCTGGACAA GTTATAGTCA TGGTTCTTGG TGATGAGAAG 
TCAGACCATA TGATTGATGT GGCCACAGTG GACCTGCTCC AGGACAGAGA GGGCTTCACA 
TGGAAGGGTC ACGACCGGCT GCCTCCTAAA TCGGGCTCAG TAGTGTTTGC TCCTGGATTC 
CAGCCGGTGG TCCTAGTTCA ATGCCTGCCG CCCGCCGCCG TTACTGCAGT GACCCTGCAT 
GCGGAGTGGA ATCTCATTGC CTTTGGCACT AGTCATGGCT TTGGCCTTTT TGACTACCAC 
CGGCGAAGTC CAGTTCTGGC GAGATGCACG CTGCACCCCA ATGACTCTCT GGCCATGGAG 
GGGCCACTGT CCCGAGTGAA GTCCTTGAAG AAGTCCCTGC GCCAGTCCTT CAGGAGGATC 
CGCAAGAGCA GAGTCTCAGG AAAGAAGCGC GTCGTGGTCA ACAGTCCCAC CAGTAAAGTG 
CAAGAGGCGA ACGCTCATTT GGCAGATCAC GATGCTGAAG TGACACCTGT GCAGAGGAGG 
ATTGAGCCCC GTTCAGCGGA TGACTCTCTG TCGGGAGTGG TGCGATGCCT CTGTTTCGCT 
GACACCTTCC TTAGAGACGG AACACATCAT GGTCCTACAA TGTGGGCCGG CACAAACTCT 
GGTTCAGTGT ACGCTTACGC TCCGGACGTC CCGTCACAGG AGAAGTTCTC TGAGCAGAGT 
GTGGAGGCCC TGCTGGGGAA GGAGATCCAG CTGATGCACA GAGCTCCAGT GGTGTCCATC 
GCTGTGCTGG ACGGCCGAGG AAACCCGCTG CCAGAGCCTT ATGAAGTGTC CAGAGATCTG 
GCCAAAGCCC CCGACATGCA GGGAAGCCAC TCGGTTCTTA TCGCTTCTGA GGAGCAGTTT 
AAGGTTTTCA TGCTGCCAAA AGTAAGTGCG AAGACCAAGT TCAAGCTGAC AGCTCATGAG 
GGCTGTCGGG TGCGTAAGGT GGCACTGGCG AACTTCACCA GCGTGAGCTC TGAAGATTAC 
TCTGAGAATG CTCTGGTGTG CCTTACCAAC ATGGGCGACA TCCACATGTT CAGTGTGCCG 
GCTCTCAGAC CGCAAGTGCG TTATGACTGC GTCCGCAAGG AAGACATCAG TGGCATCGCT 
TCTTGTGTTT TCACCAAGAC TGGACAAGGA TTCTACTTGA TTTCTCCATC GGAGTATGAG 
CGATTCTCTC TGTCGGCTCG TGTGATCACA GAGCCGCTGT GTGGAGTGGA TATAGAGCGA 
CCTCACGATG TCTCTGCGCA CAGTTCCAGC ACGACGCTTC CACCGCAGGC TAATGGAACA 
CACAAGTCAC AAGCAGAGAG CAAGTCTGCT GAAACTGAAG ATTTGTTGGA GGAAATGCGC 
AGTGTCTTGT CTTCTCCCAT CCTGGATTCT CCAAACAGCA GTGCGGACAT CACTCTGGAC 
ACCACTGGAG ATCTGACTGT GGAGGACGTC AAAGACTTCT TAATGACTGT GGATGAAGCG 
GAGAATAACT TCAGGAACAT AACTGAGGAG GACGGGAGAT CTGCTGGAAT CCTCATCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038934.2
CDS	1..3123
Translation	MMKFRFRRQGNDPHREKIKQDLFAFNKTVEHGFPNQPSALAYDPKLQLMAIGTKSGAIKIYGAPGVEMTGLHKDTAAVTQIHFLCGQGRILSLLDDNTIHLWEIVQRENRSHLLEVHSFNLPGRPGIESTSATRVTVMLLNLSCNLLALGTEGGGVHFLELPTLTLLNKSLFQDEIMQSVPEEYKCGKSLGPVESLQEHPKDSDKILIGYSRGLVVLWDLSSRHVDNLFLGKQQLESLVWERSGNSFVSSHSDGGYMVWAVSSSNPCTHDPVSSTIPYGPFPCKAVNKILWRTTESGAPLVLFSGGMPRASYGDRHCLTILQDSSHVTLDFTSRVIDFFTIHCTDTEKDFDEPSALVVLLEEELVVIDLQMPGWPTVPAPYLAPLHSSAITCSCHISNVPPKLWERVISAGQQQCPLQNYENWPICGGKNLAPDPKQKELLLTGHEDGTVRFWEASGVSLKPLYKLSTASIFQTDCEHNDSLTQAGEEEWPPFRKVGCFDPYSDDPRLGIQKISLCKYSGKLVVAGTAGQVIVMVLGDEKSDHMIDVATVDLLQDREGFTWKGHDRLPPKSGSVVFAPGFQPVVLVQCLPPAAVTAVTLHAEWNLIAFGTSHGFGLFDYHRRSPVLARCTLHPNDSLAMEGPLSRVKSLKKSLRQSFRRIRKSRVSGKKRVVVNSPTSKVQEANAHLADHDAEVTPVQRRIEPRSADDSLSGVVRCLCFADTFLRDGTHHGPTMWAGTNSGSVYAYAPDVPSQEKFSEQSVEALLGKEIQLMHRAPVVSIAVLDGRGNPLPEPYEVSRDLAKAPDMQGSHSVLIASEEQFKVFMLPKVSAKTKFKLTAHEGCRVRKVALANFTSVSSEDYSENALVCLTNMGDIHMFSVPALRPQVRYDCVRKEDISGIASCVFTKTGQGFYLISPSEYERFSLSARVITEPLCGVDIERPHDVSAHSSSTTLPPQANGTHKSQAESKSAETEDLLEEMRSVLSSPILDSPNSSADITLDTTGDLTVEDVKDFLMTVDEAENNFRNITEEDGRSAGILIN

Target ORF information:

RefSeq Version	NM_001045469.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio LLGL scribble cell polarity complex component 1 (llgl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045469.2

ORF Insert Sequence:

ATGATGAAGT TTAGGTTCAG ACGGCAGGGA AATGACCCTC ATCGTGAAAA AATCAAGCAG 
GATCTGTTTG CATTCAATAA GACAGTTGAA CATGGATTTC CAAACCAGCC AAGTGCTTTG 
GCCTATGATC CCAAACTGCA GCTTATGGCT ATTGGAACTA AATCAGGGGC CATCAAAATA 
TATGGTGCTC CAGGGGTAGA GATGACGGGA CTTCATAAAG ACACTGCTGC TGTTACGCAG 
ATACACTTCC TCTGTGGACA GGGAAGAATA CTTTCTCTTT TGGATGATAA CACGATTCAC 
CTTTGGGAGA TCGTGCAGAG AGAAAACCGA TCACATTTGC TAGAAGTTCA CAGCTTCAAC 
CTGCCTGGCA GGCCGGGCAT CGAGAGCACA AGTGCAACTC GTGTGACGGT CATGCTGCTG 
AATCTGAGCT GTAATCTTCT CGCTCTGGGG ACTGAAGGTG GTGGGGTTCA CTTTCTGGAG 
TTACCTACCC TCACACTGCT CAACAAGTCT CTGTTTCAGG ACGAGATCAT GCAGAGCGTT 
CCAGAGGAGT ACAAGTGTGG AAAATCATTG GGGCCTGTGG AGTCCCTGCA GGAACATCCG 
AAAGACTCAG ATAAAATCCT CATTGGCTAC AGCAGAGGTC TGGTGGTGCT CTGGGACCTC 
AGCAGTCGGC ATGTGGACAA CCTCTTCCTG GGAAAGCAGC AGCTGGAGAG TTTGGTCTGG 
GAACGGTCCG GGAACAGCTT TGTTAGTTCT CATAGTGATG GCGGATACAT GGTGTGGGCT 
GTCAGCAGCA GCAATCCCTG CACCCATGAC CCCGTCTCAT CCACTATACC ATATGGCCCC 
TTTCCTTGCA AAGCCGTGAA CAAGATCCTG TGGAGGACGA CAGAATCAGG AGCTCCTCTA 
GTGTTATTCA GTGGCGGGAT GCCCAGAGCC AGCTATGGAG ACAGACACTG CCTCACCATC 
CTGCAGGACA GCAGCCACGT CACGCTGGAC TTCACCTCGC GTGTCATCGA CTTCTTCACT 
ATTCACTGCA CGGATACAGA GAAGGACTTT GACGAGCCTT CAGCACTGGT GGTTCTTCTG 
GAGGAGGAGC TGGTGGTGAT TGATCTTCAG ATGCCTGGAT GGCCCACAGT CCCTGCTCCA 
TATCTGGCTC CTCTGCACTC TTCCGCCATC ACCTGCTCCT GCCACATCTC CAATGTGCCT 
CCCAAACTGT GGGAGCGAGT GATCAGTGCT GGCCAACAGC AGTGTCCGCT GCAGAACTAT 
GAGAATTGGC CAATATGTGG AGGGAAAAAC TTAGCGCCTG ATCCAAAACA GAAGGAACTG 
CTGTTAACTG GTCATGAGGA CGGAACAGTG CGTTTCTGGG AGGCGTCAGG AGTGTCACTC 
AAACCTTTAT ACAAACTGAG CACGGCCTCC ATCTTTCAGA CAGACTGCGA GCACAACGAC 
AGCCTGACAC AAGCCGGAGA GGAGGAATGG CCTCCATTCA GAAAGGTGGG CTGCTTTGAC 
CCATACAGTG ATGACCCCAG ACTTGGAATC CAGAAAATCA GCCTCTGTAA ATACAGTGGA 
AAACTGGTGG TGGCTGGAAC TGCTGGACAA GTTATAGTCA TGGTTCTTGG TGATGAGAAG 
TCAGACCATA TGATTGATGT GGCCACAGTG GACCTGCTCC AGGACAGAGA GGGCTTCACA 
TGGAAGGGTC ACGACCGGCT GCCTCCTAAA TCGGGCTCAG TAGTGTTTGC TCCTGGATTC 
CAGCCGGTGG TCCTAGTTCA ATGCCTGCCG CCCGCCGCCG TTACTGCAGT GACCCTGCAT 
GCGGAGTGGA ATCTCATTGC CTTTGGCACT AGTCATGGCT TTGGCCTTTT TGACTACCAC 
CGGCGAAGTC CAGTTCTGGC GAGATGCACG CTGCACCCCA ATGACTCTCT GGCCATGGAG 
GGGCCACTGT CCCGAGTGAA GTCCTTGAAG AAGTCCCTGC GCCAGTCCTT CAGGAGGATC 
CGCAAGAGCA GAGTCTCAGG AAAGAAGCGC GTCGTGGTCA ACAGTCCCAC CAGTAAAGTG 
CAAGAGGCGA ACGCTCATTT GGCAGATCAC GATGCTGAAG TGACACCTGT GCAGAGGAGG 
ATTGAGCCCC GTTCAGCGGA TGACTCTCTG TCGGGAGTGG TGCGATGCCT CTGTTTCGCT 
GACACCTTCC TTAGAGACGG AACACATCAT GGTCCTACAA TGTGGGCCGG CACAAACTCT 
GGTTCAGTGT ACGCTTACGC TCCGGACGTC CCGTCACAGG AGAAGTTCTC TGAGCAGAGT 
GTGGAGGCCC TGCTGGGGAA GGAGATCCAG CTGATGCACA GAGCTCCAGT GGTGTCCATC 
GCTGTGCTGG ACGGCCGAGG AAACCCGCTG CCAGAGCCTT ATGAAGTGTC CAGAGATCTG 
GCCAAAGCCC CCGACATGCA GGGAAGCCAC TCGGTTCTTA TCGCTTCTGA GGAGCAGTTT 
AAGGTTTTCA TGCTGCCAAA AGTAAGTGCG AAGACCAAGT TCAAGCTGAC AGCTCATGAG 
GGCTGTCGGG TGCGTAAGGT GGCACTGGCG AACTTCACCA GCGTGAGCTC TGAAGATTAC 
TCTGAGAATG CTCTGGTGTG CCTTACCAAC ATGGGCGACA TCCACATGTT CAGTGTGCCG 
GCTCTCAGAC CGCAAGTGCG TTATGACTGC GTCCGCAAGG AAGACATCAG TGGCATCGCT 
TCTTGTGTTT TCACCAAGAC TGGACAAGGA TTCTACTTGA TTTCTCCATC GGAGTATGAG 
CGATTCTCTC TGTCGGCTCG TGTGATCACA GAGCCGCTGT GTGGAGTGGA TATAGAGCGA 
CCTCACGATG TCTCTGCGCA CAGTTCCAGC ACGACGCTTC CACCGCAGGC TAATGGAACA 
CACAAGTCAC AAGCAGAGAG CAAGTCTGCT GAAACTGAAG ATTTGTTGGA GGAAATGCGC 
AGTGTCTTGT CTTCTCCCAT CCTGGATTCT CCAAACAGCA GTGCGGACAT CACTCTGGAC 
ACCACTGGAG ATCTGACTGT GGAGGACGTC AAAGACTTCT TAATGACTGT GGATGAAGCG 
GAGAATAACT TCAGGAACAT AACTGAGGAG GACGGGAGAT CTGCTGGAAT CCTCATCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LLGL1 ( XM_005164141.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005164198.1 Danio rerio lethal giant larvae homolog 1 (Drosophila) (llgl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08598
Clone ID Related Accession (Same CDS sequence)	XM_005164141.3 , XM_005164141.4
Accession Version	XM_005164141.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	lethal(2) giant larvae protein homolog 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164141.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGT TTAGGTTCAG ACGGCAGGGA AATGACCCTC ATCGTGAAAA AATCAAGCAG 
GATCTGTTTG CATTCAATAA GACAGTTGAA CATGGATTTC CAAACCAGCC AAGTGCTTTG 
GCCTATGATC CCAAACTGCA GCTTATGGCT ATTGGAACTA AATCAGGGGC CATCAAAATA 
TATGGTGCTC CAGGGGTAGA GATGACGGGA CTTCATAAAG ACACTGCTGC TGTTACGCAG 
ATACACTTCC TCTGTGGACA GGGAAGAATA CTTTCTCTTT TGGATGATAA CACGATTCAC 
CTTTGGGAGA TCGTGCAGAG AGAAAACCGA TCACATTTGC TAGAAGTTCA CAGCTTCAAC 
CTGCCTGGCA GGCCGGGCAT CGAGAGCACA AGTGCAACTC GTGTGACGGT CATGCTGCTG 
AATCTGAGCT GTAATCTTCT CGCTCTGGGG ACTGAAGGTG GTGGGGTTCA CTTTCTGGAG 
TTGCCTACCC TCACACTGCT CAACAAGTCT CTGTTTCAGG ACGAGATCAT GCAGAGCGTT 
CCAGAGGAGT ACAAGTGTGG AAAATCATTG GGGCCTGTGG AGTCCCTGCA GGAACATCCG 
AAAGACTCAG ATAAAATCCT CATTGGCTAC AGCAGAGGTC TGGTGGTGCT CTGGGACCTC 
AGCAGTCGGC ATGTGGACAA CCTCTTCCTG GGAAAGCAGC AGCTGGAGAG TTTGGTCTGG 
GAACGGTCCG GGAACAGCTT TGTTAGTTCT CATAGTGATG GCGGATACAT GGTGTGGGCT 
GTCAGCAGCA GCAATCCCTG CACCCATGAC CCCGTCTCAT CCACTATACC ATATGGCCCC 
TTTCCTTGCA AAGCCGTGAA CAAGATCCTG TGGAGGACGA CAGAATCAGG AGCTCCTCTA 
GTGTTATTCA GTGGCGGGAT GCCCAGAGCC AGCTATGGAG ACAGACACTG CCTCACCATC 
CTGCAGGACA GCAGCCACGT CACGCTGGAC TTCACCTCGC GTGTCATCGA CTTCTTCACT 
ATTCACTGCA CGGATACAGA GAAGGACTTT GACGAGCCTT CAGCACTGGT GGTTCTTCTG 
GAGGAGGAGC TGGTGGTGAT TGATCTTCAG ATGCCTGGAT GGCCCACAGT CCCTGCTCCA 
TATCTGGCTC CTCTGCACTC TTCCGCCATC ACCTGCTCCT GCCACATCTC CAATGTGCCT 
CCCAAACTGT GGGAGCGAGT GATCAGTGCT GGCCAACAGC AGTGTCCGCT GCAGAACTAT 
GAGAATTGGC CAATATGTGG AGGGAAAAAC TTAGCGCCTG ATCCAAAACA GAAGGAACTG 
CTGTTAACTG GTCATGAGGA CGGAACAGTG CGTTTCTGGG ATGCGTCAGG AGTGTCACTC 
AAACCTTTAT ACAAACTGAG CACGGCCTCC ATCTTTCAGA CAGACTGCGA GCACAACGAC 
AGCCTGACAC AAGCCGGAGA GGAGGAATGG CCTCCATTCA GAAAGGTGGG CTGCTTTGAC 
CCATACAGTG ATGACCCCAG ACTTGGAATC CAGAAAATCA GCCTCTGTAA ATACAGTGGA 
AAACTGGTGG TGGCTGGAAC TGCTGGACAA GTTATAGTCA TGGTTCTTGG TGATGAGAAG 
TCAGACCATA TGATTGATGT GGCCACAGTG GACCTGCTCC AGGACAGAGA GGGCTTCACA 
TGGAAGGGTC ACGACCGGCT GCCTCCTAAA TCGGGCTCAG TAGTGTTTGC TCCTGGATTC 
CAGCCGGTGG TCCTAGTTCA ATGCCTGCCG CCCGCCGCCG TTACTGCAGT GACCCTGCAT 
GCGGAGTGGA ATCTCATTGC CTTTGGCACT AGTCATGGCT TTGGCCTTTT TGACTACCAC 
CGGCGAAGTC CAGTTCTGGC GAGATGCACG CTGCACCCCA ATGACTCTCT GGCCATGGAG 
GGGCCACTGT CCCGAGTGAA GTCCTTGAAG AAGTCCCTGC GCCAGTCCTT CAGGAGGATC 
CGCAAGAGCA GAGTCTCAGG AAAGAAGCGC GTCGTGGTCA ACAGTCCCAC CAGTAAAGTG 
CAAGAGGCGA ACGCTCATTT GGCAGATCAC GATGCTGAAG TGACACCTGT GCAGAGGAGG 
ATTGAGCCCC GTTCAGCGGA TGACTCTCTG TCGGGAGTGG TGCGATGCCT CTGTTTCGCT 
GACACCTTCC TTAGAGACGG AACACATCAT GGTCCTACAA TGTGGGCCGG CACAAACTCT 
GGTTCAGTGT ACGCTTACGC TCTGGACGTC CCGTCACAGG AGAAGTTCTC TGAGCAGAGT 
GTGGAGGCCC TGCTGGGGAA GGAGATCCAG CTGATGCACA GAGCTCCAGT GGTGTCCATC 
GCTGTGCTGG ACGGCCGAGG AAACCCGCTG CCAGAGCCTT ATGAAGTGTC CAGAGATCTG 
GCCAAAGCCC CCGACATGCA GGGAAGCCAC TCGGTTCTTA TCGCTTCTGA GGAGCAGTTT 
AAGGTTTTCA TGCTGCCAAA AGTAAGTGCG AAGACCAAGT TCAAGCTGAC AGCTCATGAG 
GGCTGTCGGG TGCGTAAGGT GGCACTGGCG AACTTCACCA GCGTGAGCTC TGAAGATTAC 
TCTGAGAATG CTCTGGTGTG CCTTACCAAC ATGGGCGACA TCCACATGTT CAGTGTGCCG 
GCTCTCAGAC CGCAAGTGCG TTATGACTGC GTCCGCAAGG AAGACATCAG TGGCATCGCT 
TCTTGTGTTT TCACCAAGAC TGGACAAGGA TTCTACTTGA TTTCTCCATC GGAGTATGAG 
CGATTCTCTC TGTCGGCTCG TGTGATCACA GAGCCGCTGT GTGGAGTGGA TGTAGAGCGA 
CCTCACGATG TCTCTGCGCA CAGCAGTTCC AGCACGACGC TTCCACCGCA GGCTAATGGA 
ACACACAAGT CACAAGCAGA GAGCAAGTCT GCTGAAACTG AAGATTTGTT GGAGGAAATG 
CGCAGTGTCT TGTCTTCTCC CATCCTGGAT TCTCCAAACA GCAGTGCAGA CATCACTCTG 
GACACCACTG GAGATCTGAC TGTGGAGGAC GTCAAAGACT TCTTAATGAC TGTGGATGAA 
GCGGAGAATA ACTTCAGGAA CATAACTGAG GAGGACGGGA GATCTGCTGG AATCCTCATC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164198.1
CDS	157..3282
Translation	MMKFRFRRQGNDPHREKIKQDLFAFNKTVEHGFPNQPSALAYDPKLQLMAIGTKSGAIKIYGAPGVEMTGLHKDTAAVTQIHFLCGQGRILSLLDDNTIHLWEIVQRENRSHLLEVHSFNLPGRPGIESTSATRVTVMLLNLSCNLLALGTEGGGVHFLELPTLTLLNKSLFQDEIMQSVPEEYKCGKSLGPVESLQEHPKDSDKILIGYSRGLVVLWDLSSRHVDNLFLGKQQLESLVWERSGNSFVSSHSDGGYMVWAVSSSNPCTHDPVSSTIPYGPFPCKAVNKILWRTTESGAPLVLFSGGMPRASYGDRHCLTILQDSSHVTLDFTSRVIDFFTIHCTDTEKDFDEPSALVVLLEEELVVIDLQMPGWPTVPAPYLAPLHSSAITCSCHISNVPPKLWERVISAGQQQCPLQNYENWPICGGKNLAPDPKQKELLLTGHEDGTVRFWDASGVSLKPLYKLSTASIFQTDCEHNDSLTQAGEEEWPPFRKVGCFDPYSDDPRLGIQKISLCKYSGKLVVAGTAGQVIVMVLGDEKSDHMIDVATVDLLQDREGFTWKGHDRLPPKSGSVVFAPGFQPVVLVQCLPPAAVTAVTLHAEWNLIAFGTSHGFGLFDYHRRSPVLARCTLHPNDSLAMEGPLSRVKSLKKSLRQSFRRIRKSRVSGKKRVVVNSPTSKVQEANAHLADHDAEVTPVQRRIEPRSADDSLSGVVRCLCFADTFLRDGTHHGPTMWAGTNSGSVYAYALDVPSQEKFSEQSVEALLGKEIQLMHRAPVVSIAVLDGRGNPLPEPYEVSRDLAKAPDMQGSHSVLIASEEQFKVFMLPKVSAKTKFKLTAHEGCRVRKVALANFTSVSSEDYSENALVCLTNMGDIHMFSVPALRPQVRYDCVRKEDISGIASCVFTKTGQGFYLISPSEYERFSLSARVITEPLCGVDVERPHDVSAHSSSSTTLPPQANGTHKSQAESKSAETEDLLEEMRSVLSSPILDSPNSSADITLDTTGDLTVEDVKDFLMTVDEAENNFRNITEEDGRSAGILIN

Target ORF information:

RefSeq Version	XM_005164141.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lethal giant larvae homolog 1 (Drosophila) (llgl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164141.3

ORF Insert Sequence:

ATGATGAAGT TTAGGTTCAG ACGGCAGGGA AATGACCCTC ATCGTGAAAA AATCAAGCAG 
GATCTGTTTG CATTCAATAA GACAGTTGAA CATGGATTTC CAAACCAGCC AAGTGCTTTG 
GCCTATGATC CCAAACTGCA GCTTATGGCT ATTGGAACTA AATCAGGGGC CATCAAAATA 
TATGGTGCTC CAGGGGTAGA GATGACGGGA CTTCATAAAG ACACTGCTGC TGTTACGCAG 
ATACACTTCC TCTGTGGACA GGGAAGAATA CTTTCTCTTT TGGATGATAA CACGATTCAC 
CTTTGGGAGA TCGTGCAGAG AGAAAACCGA TCACATTTGC TAGAAGTTCA CAGCTTCAAC 
CTGCCTGGCA GGCCGGGCAT CGAGAGCACA AGTGCAACTC GTGTGACGGT CATGCTGCTG 
AATCTGAGCT GTAATCTTCT CGCTCTGGGG ACTGAAGGTG GTGGGGTTCA CTTTCTGGAG 
TTGCCTACCC TCACACTGCT CAACAAGTCT CTGTTTCAGG ACGAGATCAT GCAGAGCGTT 
CCAGAGGAGT ACAAGTGTGG AAAATCATTG GGGCCTGTGG AGTCCCTGCA GGAACATCCG 
AAAGACTCAG ATAAAATCCT CATTGGCTAC AGCAGAGGTC TGGTGGTGCT CTGGGACCTC 
AGCAGTCGGC ATGTGGACAA CCTCTTCCTG GGAAAGCAGC AGCTGGAGAG TTTGGTCTGG 
GAACGGTCCG GGAACAGCTT TGTTAGTTCT CATAGTGATG GCGGATACAT GGTGTGGGCT 
GTCAGCAGCA GCAATCCCTG CACCCATGAC CCCGTCTCAT CCACTATACC ATATGGCCCC 
TTTCCTTGCA AAGCCGTGAA CAAGATCCTG TGGAGGACGA CAGAATCAGG AGCTCCTCTA 
GTGTTATTCA GTGGCGGGAT GCCCAGAGCC AGCTATGGAG ACAGACACTG CCTCACCATC 
CTGCAGGACA GCAGCCACGT CACGCTGGAC TTCACCTCGC GTGTCATCGA CTTCTTCACT 
ATTCACTGCA CGGATACAGA GAAGGACTTT GACGAGCCTT CAGCACTGGT GGTTCTTCTG 
GAGGAGGAGC TGGTGGTGAT TGATCTTCAG ATGCCTGGAT GGCCCACAGT CCCTGCTCCA 
TATCTGGCTC CTCTGCACTC TTCCGCCATC ACCTGCTCCT GCCACATCTC CAATGTGCCT 
CCCAAACTGT GGGAGCGAGT GATCAGTGCT GGCCAACAGC AGTGTCCGCT GCAGAACTAT 
GAGAATTGGC CAATATGTGG AGGGAAAAAC TTAGCGCCTG ATCCAAAACA GAAGGAACTG 
CTGTTAACTG GTCATGAGGA CGGAACAGTG CGTTTCTGGG ATGCGTCAGG AGTGTCACTC 
AAACCTTTAT ACAAACTGAG CACGGCCTCC ATCTTTCAGA CAGACTGCGA GCACAACGAC 
AGCCTGACAC AAGCCGGAGA GGAGGAATGG CCTCCATTCA GAAAGGTGGG CTGCTTTGAC 
CCATACAGTG ATGACCCCAG ACTTGGAATC CAGAAAATCA GCCTCTGTAA ATACAGTGGA 
AAACTGGTGG TGGCTGGAAC TGCTGGACAA GTTATAGTCA TGGTTCTTGG TGATGAGAAG 
TCAGACCATA TGATTGATGT GGCCACAGTG GACCTGCTCC AGGACAGAGA GGGCTTCACA 
TGGAAGGGTC ACGACCGGCT GCCTCCTAAA TCGGGCTCAG TAGTGTTTGC TCCTGGATTC 
CAGCCGGTGG TCCTAGTTCA ATGCCTGCCG CCCGCCGCCG TTACTGCAGT GACCCTGCAT 
GCGGAGTGGA ATCTCATTGC CTTTGGCACT AGTCATGGCT TTGGCCTTTT TGACTACCAC 
CGGCGAAGTC CAGTTCTGGC GAGATGCACG CTGCACCCCA ATGACTCTCT GGCCATGGAG 
GGGCCACTGT CCCGAGTGAA GTCCTTGAAG AAGTCCCTGC GCCAGTCCTT CAGGAGGATC 
CGCAAGAGCA GAGTCTCAGG AAAGAAGCGC GTCGTGGTCA ACAGTCCCAC CAGTAAAGTG 
CAAGAGGCGA ACGCTCATTT GGCAGATCAC GATGCTGAAG TGACACCTGT GCAGAGGAGG 
ATTGAGCCCC GTTCAGCGGA TGACTCTCTG TCGGGAGTGG TGCGATGCCT CTGTTTCGCT 
GACACCTTCC TTAGAGACGG AACACATCAT GGTCCTACAA TGTGGGCCGG CACAAACTCT 
GGTTCAGTGT ACGCTTACGC TCTGGACGTC CCGTCACAGG AGAAGTTCTC TGAGCAGAGT 
GTGGAGGCCC TGCTGGGGAA GGAGATCCAG CTGATGCACA GAGCTCCAGT GGTGTCCATC 
GCTGTGCTGG ACGGCCGAGG AAACCCGCTG CCAGAGCCTT ATGAAGTGTC CAGAGATCTG 
GCCAAAGCCC CCGACATGCA GGGAAGCCAC TCGGTTCTTA TCGCTTCTGA GGAGCAGTTT 
AAGGTTTTCA TGCTGCCAAA AGTAAGTGCG AAGACCAAGT TCAAGCTGAC AGCTCATGAG 
GGCTGTCGGG TGCGTAAGGT GGCACTGGCG AACTTCACCA GCGTGAGCTC TGAAGATTAC 
TCTGAGAATG CTCTGGTGTG CCTTACCAAC ATGGGCGACA TCCACATGTT CAGTGTGCCG 
GCTCTCAGAC CGCAAGTGCG TTATGACTGC GTCCGCAAGG AAGACATCAG TGGCATCGCT 
TCTTGTGTTT TCACCAAGAC TGGACAAGGA TTCTACTTGA TTTCTCCATC GGAGTATGAG 
CGATTCTCTC TGTCGGCTCG TGTGATCACA GAGCCGCTGT GTGGAGTGGA TGTAGAGCGA 
CCTCACGATG TCTCTGCGCA CAGCAGTTCC AGCACGACGC TTCCACCGCA GGCTAATGGA 
ACACACAAGT CACAAGCAGA GAGCAAGTCT GCTGAAACTG AAGATTTGTT GGAGGAAATG 
CGCAGTGTCT TGTCTTCTCC CATCCTGGAT TCTCCAAACA GCAGTGCAGA CATCACTCTG 
GACACCACTG GAGATCTGAC TGTGGAGGAC GTCAAAGACT TCTTAATGAC TGTGGATGAA 
GCGGAGAATA ACTTCAGGAA CATAACTGAG GAGGACGGGA GATCTGCTGG AATCCTCATC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LLGL1 ( XM_005164141.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005164198.1 Danio rerio lethal giant larvae homolog 1 (Drosophila) (llgl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08598
Clone ID Related Accession (Same CDS sequence)	XM_005164141.3 , XM_005164141.4
Accession Version	XM_005164141.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	lethal(2) giant larvae protein homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164141.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGT TTAGGTTCAG ACGGCAGGGA AATGACCCTC ATCGTGAAAA AATCAAGCAG 
GATCTGTTTG CATTCAATAA GACAGTTGAA CATGGATTTC CAAACCAGCC AAGTGCTTTG 
GCCTATGATC CCAAACTGCA GCTTATGGCT ATTGGAACTA AATCAGGGGC CATCAAAATA 
TATGGTGCTC CAGGGGTAGA GATGACGGGA CTTCATAAAG ACACTGCTGC TGTTACGCAG 
ATACACTTCC TCTGTGGACA GGGAAGAATA CTTTCTCTTT TGGATGATAA CACGATTCAC 
CTTTGGGAGA TCGTGCAGAG AGAAAACCGA TCACATTTGC TAGAAGTTCA CAGCTTCAAC 
CTGCCTGGCA GGCCGGGCAT CGAGAGCACA AGTGCAACTC GTGTGACGGT CATGCTGCTG 
AATCTGAGCT GTAATCTTCT CGCTCTGGGG ACTGAAGGTG GTGGGGTTCA CTTTCTGGAG 
TTGCCTACCC TCACACTGCT CAACAAGTCT CTGTTTCAGG ACGAGATCAT GCAGAGCGTT 
CCAGAGGAGT ACAAGTGTGG AAAATCATTG GGGCCTGTGG AGTCCCTGCA GGAACATCCG 
AAAGACTCAG ATAAAATCCT CATTGGCTAC AGCAGAGGTC TGGTGGTGCT CTGGGACCTC 
AGCAGTCGGC ATGTGGACAA CCTCTTCCTG GGAAAGCAGC AGCTGGAGAG TTTGGTCTGG 
GAACGGTCCG GGAACAGCTT TGTTAGTTCT CATAGTGATG GCGGATACAT GGTGTGGGCT 
GTCAGCAGCA GCAATCCCTG CACCCATGAC CCCGTCTCAT CCACTATACC ATATGGCCCC 
TTTCCTTGCA AAGCCGTGAA CAAGATCCTG TGGAGGACGA CAGAATCAGG AGCTCCTCTA 
GTGTTATTCA GTGGCGGGAT GCCCAGAGCC AGCTATGGAG ACAGACACTG CCTCACCATC 
CTGCAGGACA GCAGCCACGT CACGCTGGAC TTCACCTCGC GTGTCATCGA CTTCTTCACT 
ATTCACTGCA CGGATACAGA GAAGGACTTT GACGAGCCTT CAGCACTGGT GGTTCTTCTG 
GAGGAGGAGC TGGTGGTGAT TGATCTTCAG ATGCCTGGAT GGCCCACAGT CCCTGCTCCA 
TATCTGGCTC CTCTGCACTC TTCCGCCATC ACCTGCTCCT GCCACATCTC CAATGTGCCT 
CCCAAACTGT GGGAGCGAGT GATCAGTGCT GGCCAACAGC AGTGTCCGCT GCAGAACTAT 
GAGAATTGGC CAATATGTGG AGGGAAAAAC TTAGCGCCTG ATCCAAAACA GAAGGAACTG 
CTGTTAACTG GTCATGAGGA CGGAACAGTG CGTTTCTGGG ATGCGTCAGG AGTGTCACTC 
AAACCTTTAT ACAAACTGAG CACGGCCTCC ATCTTTCAGA CAGACTGCGA GCACAACGAC 
AGCCTGACAC AAGCCGGAGA GGAGGAATGG CCTCCATTCA GAAAGGTGGG CTGCTTTGAC 
CCATACAGTG ATGACCCCAG ACTTGGAATC CAGAAAATCA GCCTCTGTAA ATACAGTGGA 
AAACTGGTGG TGGCTGGAAC TGCTGGACAA GTTATAGTCA TGGTTCTTGG TGATGAGAAG 
TCAGACCATA TGATTGATGT GGCCACAGTG GACCTGCTCC AGGACAGAGA GGGCTTCACA 
TGGAAGGGTC ACGACCGGCT GCCTCCTAAA TCGGGCTCAG TAGTGTTTGC TCCTGGATTC 
CAGCCGGTGG TCCTAGTTCA ATGCCTGCCG CCCGCCGCCG TTACTGCAGT GACCCTGCAT 
GCGGAGTGGA ATCTCATTGC CTTTGGCACT AGTCATGGCT TTGGCCTTTT TGACTACCAC 
CGGCGAAGTC CAGTTCTGGC GAGATGCACG CTGCACCCCA ATGACTCTCT GGCCATGGAG 
GGGCCACTGT CCCGAGTGAA GTCCTTGAAG AAGTCCCTGC GCCAGTCCTT CAGGAGGATC 
CGCAAGAGCA GAGTCTCAGG AAAGAAGCGC GTCGTGGTCA ACAGTCCCAC CAGTAAAGTG 
CAAGAGGCGA ACGCTCATTT GGCAGATCAC GATGCTGAAG TGACACCTGT GCAGAGGAGG 
ATTGAGCCCC GTTCAGCGGA TGACTCTCTG TCGGGAGTGG TGCGATGCCT CTGTTTCGCT 
GACACCTTCC TTAGAGACGG AACACATCAT GGTCCTACAA TGTGGGCCGG CACAAACTCT 
GGTTCAGTGT ACGCTTACGC TCTGGACGTC CCGTCACAGG AGAAGTTCTC TGAGCAGAGT 
GTGGAGGCCC TGCTGGGGAA GGAGATCCAG CTGATGCACA GAGCTCCAGT GGTGTCCATC 
GCTGTGCTGG ACGGCCGAGG AAACCCGCTG CCAGAGCCTT ATGAAGTGTC CAGAGATCTG 
GCCAAAGCCC CCGACATGCA GGGAAGCCAC TCGGTTCTTA TCGCTTCTGA GGAGCAGTTT 
AAGGTTTTCA TGCTGCCAAA AGTAAGTGCG AAGACCAAGT TCAAGCTGAC AGCTCATGAG 
GGCTGTCGGG TGCGTAAGGT GGCACTGGCG AACTTCACCA GCGTGAGCTC TGAAGATTAC 
TCTGAGAATG CTCTGGTGTG CCTTACCAAC ATGGGCGACA TCCACATGTT CAGTGTGCCG 
GCTCTCAGAC CGCAAGTGCG TTATGACTGC GTCCGCAAGG AAGACATCAG TGGCATCGCT 
TCTTGTGTTT TCACCAAGAC TGGACAAGGA TTCTACTTGA TTTCTCCATC GGAGTATGAG 
CGATTCTCTC TGTCGGCTCG TGTGATCACA GAGCCGCTGT GTGGAGTGGA TGTAGAGCGA 
CCTCACGATG TCTCTGCGCA CAGCAGTTCC AGCACGACGC TTCCACCGCA GGCTAATGGA 
ACACACAAGT CACAAGCAGA GAGCAAGTCT GCTGAAACTG AAGATTTGTT GGAGGAAATG 
CGCAGTGTCT TGTCTTCTCC CATCCTGGAT TCTCCAAACA GCAGTGCAGA CATCACTCTG 
GACACCACTG GAGATCTGAC TGTGGAGGAC GTCAAAGACT TCTTAATGAC TGTGGATGAA 
GCGGAGAATA ACTTCAGGAA CATAACTGAG GAGGACGGGA GATCTGCTGG AATCCTCATC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164198.1
CDS	175..3300
Translation	MMKFRFRRQGNDPHREKIKQDLFAFNKTVEHGFPNQPSALAYDPKLQLMAIGTKSGAIKIYGAPGVEMTGLHKDTAAVTQIHFLCGQGRILSLLDDNTIHLWEIVQRENRSHLLEVHSFNLPGRPGIESTSATRVTVMLLNLSCNLLALGTEGGGVHFLELPTLTLLNKSLFQDEIMQSVPEEYKCGKSLGPVESLQEHPKDSDKILIGYSRGLVVLWDLSSRHVDNLFLGKQQLESLVWERSGNSFVSSHSDGGYMVWAVSSSNPCTHDPVSSTIPYGPFPCKAVNKILWRTTESGAPLVLFSGGMPRASYGDRHCLTILQDSSHVTLDFTSRVIDFFTIHCTDTEKDFDEPSALVVLLEEELVVIDLQMPGWPTVPAPYLAPLHSSAITCSCHISNVPPKLWERVISAGQQQCPLQNYENWPICGGKNLAPDPKQKELLLTGHEDGTVRFWDASGVSLKPLYKLSTASIFQTDCEHNDSLTQAGEEEWPPFRKVGCFDPYSDDPRLGIQKISLCKYSGKLVVAGTAGQVIVMVLGDEKSDHMIDVATVDLLQDREGFTWKGHDRLPPKSGSVVFAPGFQPVVLVQCLPPAAVTAVTLHAEWNLIAFGTSHGFGLFDYHRRSPVLARCTLHPNDSLAMEGPLSRVKSLKKSLRQSFRRIRKSRVSGKKRVVVNSPTSKVQEANAHLADHDAEVTPVQRRIEPRSADDSLSGVVRCLCFADTFLRDGTHHGPTMWAGTNSGSVYAYALDVPSQEKFSEQSVEALLGKEIQLMHRAPVVSIAVLDGRGNPLPEPYEVSRDLAKAPDMQGSHSVLIASEEQFKVFMLPKVSAKTKFKLTAHEGCRVRKVALANFTSVSSEDYSENALVCLTNMGDIHMFSVPALRPQVRYDCVRKEDISGIASCVFTKTGQGFYLISPSEYERFSLSARVITEPLCGVDVERPHDVSAHSSSSTTLPPQANGTHKSQAESKSAETEDLLEEMRSVLSSPILDSPNSSADITLDTTGDLTVEDVKDFLMTVDEAENNFRNITEEDGRSAGILIN

Target ORF information:

RefSeq Version	XM_005164141.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lethal giant larvae homolog 1 (Drosophila) (llgl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164141.4

ORF Insert Sequence:

ATGATGAAGT TTAGGTTCAG ACGGCAGGGA AATGACCCTC ATCGTGAAAA AATCAAGCAG 
GATCTGTTTG CATTCAATAA GACAGTTGAA CATGGATTTC CAAACCAGCC AAGTGCTTTG 
GCCTATGATC CCAAACTGCA GCTTATGGCT ATTGGAACTA AATCAGGGGC CATCAAAATA 
TATGGTGCTC CAGGGGTAGA GATGACGGGA CTTCATAAAG ACACTGCTGC TGTTACGCAG 
ATACACTTCC TCTGTGGACA GGGAAGAATA CTTTCTCTTT TGGATGATAA CACGATTCAC 
CTTTGGGAGA TCGTGCAGAG AGAAAACCGA TCACATTTGC TAGAAGTTCA CAGCTTCAAC 
CTGCCTGGCA GGCCGGGCAT CGAGAGCACA AGTGCAACTC GTGTGACGGT CATGCTGCTG 
AATCTGAGCT GTAATCTTCT CGCTCTGGGG ACTGAAGGTG GTGGGGTTCA CTTTCTGGAG 
TTGCCTACCC TCACACTGCT CAACAAGTCT CTGTTTCAGG ACGAGATCAT GCAGAGCGTT 
CCAGAGGAGT ACAAGTGTGG AAAATCATTG GGGCCTGTGG AGTCCCTGCA GGAACATCCG 
AAAGACTCAG ATAAAATCCT CATTGGCTAC AGCAGAGGTC TGGTGGTGCT CTGGGACCTC 
AGCAGTCGGC ATGTGGACAA CCTCTTCCTG GGAAAGCAGC AGCTGGAGAG TTTGGTCTGG 
GAACGGTCCG GGAACAGCTT TGTTAGTTCT CATAGTGATG GCGGATACAT GGTGTGGGCT 
GTCAGCAGCA GCAATCCCTG CACCCATGAC CCCGTCTCAT CCACTATACC ATATGGCCCC 
TTTCCTTGCA AAGCCGTGAA CAAGATCCTG TGGAGGACGA CAGAATCAGG AGCTCCTCTA 
GTGTTATTCA GTGGCGGGAT GCCCAGAGCC AGCTATGGAG ACAGACACTG CCTCACCATC 
CTGCAGGACA GCAGCCACGT CACGCTGGAC TTCACCTCGC GTGTCATCGA CTTCTTCACT 
ATTCACTGCA CGGATACAGA GAAGGACTTT GACGAGCCTT CAGCACTGGT GGTTCTTCTG 
GAGGAGGAGC TGGTGGTGAT TGATCTTCAG ATGCCTGGAT GGCCCACAGT CCCTGCTCCA 
TATCTGGCTC CTCTGCACTC TTCCGCCATC ACCTGCTCCT GCCACATCTC CAATGTGCCT 
CCCAAACTGT GGGAGCGAGT GATCAGTGCT GGCCAACAGC AGTGTCCGCT GCAGAACTAT 
GAGAATTGGC CAATATGTGG AGGGAAAAAC TTAGCGCCTG ATCCAAAACA GAAGGAACTG 
CTGTTAACTG GTCATGAGGA CGGAACAGTG CGTTTCTGGG ATGCGTCAGG AGTGTCACTC 
AAACCTTTAT ACAAACTGAG CACGGCCTCC ATCTTTCAGA CAGACTGCGA GCACAACGAC 
AGCCTGACAC AAGCCGGAGA GGAGGAATGG CCTCCATTCA GAAAGGTGGG CTGCTTTGAC 
CCATACAGTG ATGACCCCAG ACTTGGAATC CAGAAAATCA GCCTCTGTAA ATACAGTGGA 
AAACTGGTGG TGGCTGGAAC TGCTGGACAA GTTATAGTCA TGGTTCTTGG TGATGAGAAG 
TCAGACCATA TGATTGATGT GGCCACAGTG GACCTGCTCC AGGACAGAGA GGGCTTCACA 
TGGAAGGGTC ACGACCGGCT GCCTCCTAAA TCGGGCTCAG TAGTGTTTGC TCCTGGATTC 
CAGCCGGTGG TCCTAGTTCA ATGCCTGCCG CCCGCCGCCG TTACTGCAGT GACCCTGCAT 
GCGGAGTGGA ATCTCATTGC CTTTGGCACT AGTCATGGCT TTGGCCTTTT TGACTACCAC 
CGGCGAAGTC CAGTTCTGGC GAGATGCACG CTGCACCCCA ATGACTCTCT GGCCATGGAG 
GGGCCACTGT CCCGAGTGAA GTCCTTGAAG AAGTCCCTGC GCCAGTCCTT CAGGAGGATC 
CGCAAGAGCA GAGTCTCAGG AAAGAAGCGC GTCGTGGTCA ACAGTCCCAC CAGTAAAGTG 
CAAGAGGCGA ACGCTCATTT GGCAGATCAC GATGCTGAAG TGACACCTGT GCAGAGGAGG 
ATTGAGCCCC GTTCAGCGGA TGACTCTCTG TCGGGAGTGG TGCGATGCCT CTGTTTCGCT 
GACACCTTCC TTAGAGACGG AACACATCAT GGTCCTACAA TGTGGGCCGG CACAAACTCT 
GGTTCAGTGT ACGCTTACGC TCTGGACGTC CCGTCACAGG AGAAGTTCTC TGAGCAGAGT 
GTGGAGGCCC TGCTGGGGAA GGAGATCCAG CTGATGCACA GAGCTCCAGT GGTGTCCATC 
GCTGTGCTGG ACGGCCGAGG AAACCCGCTG CCAGAGCCTT ATGAAGTGTC CAGAGATCTG 
GCCAAAGCCC CCGACATGCA GGGAAGCCAC TCGGTTCTTA TCGCTTCTGA GGAGCAGTTT 
AAGGTTTTCA TGCTGCCAAA AGTAAGTGCG AAGACCAAGT TCAAGCTGAC AGCTCATGAG 
GGCTGTCGGG TGCGTAAGGT GGCACTGGCG AACTTCACCA GCGTGAGCTC TGAAGATTAC 
TCTGAGAATG CTCTGGTGTG CCTTACCAAC ATGGGCGACA TCCACATGTT CAGTGTGCCG 
GCTCTCAGAC CGCAAGTGCG TTATGACTGC GTCCGCAAGG AAGACATCAG TGGCATCGCT 
TCTTGTGTTT TCACCAAGAC TGGACAAGGA TTCTACTTGA TTTCTCCATC GGAGTATGAG 
CGATTCTCTC TGTCGGCTCG TGTGATCACA GAGCCGCTGT GTGGAGTGGA TGTAGAGCGA 
CCTCACGATG TCTCTGCGCA CAGCAGTTCC AGCACGACGC TTCCACCGCA GGCTAATGGA 
ACACACAAGT CACAAGCAGA GAGCAAGTCT GCTGAAACTG AAGATTTGTT GGAGGAAATG 
CGCAGTGTCT TGTCTTCTCC CATCCTGGAT TCTCCAAACA GCAGTGCAGA CATCACTCTG 
GACACCACTG GAGATCTGAC TGTGGAGGAC GTCAAAGACT TCTTAATGAC TGTGGATGAA 
GCGGAGAATA ACTTCAGGAA CATAACTGAG GAGGACGGGA GATCTGCTGG AATCCTCATC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LLGL1 ( XM_005164142.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005164199.1 Danio rerio lethal giant larvae homolog 1 (Drosophila) (llgl1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa08599
Clone ID Related Accession (Same CDS sequence)	XM_005164142.4 , XM_005164142.3
Accession Version	XM_005164142.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	lethal(2) giant larvae protein homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164142.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGT TTAGGTTCAG ACGGCAGGGA AATGACCCTC ATCGTGAAAA AATCAAGCAG 
GATCTGTTTG CATTCAATAA GACAGTTGAA CATGGATTTC CAAACCAGCC AAGTGCTTTG 
GCCTATGATC CCAAACTGCA GCTTATGGCT ATTGGAACTA AATCAGGGGC CATCAAAATA 
TATGGTGCTC CAGGGGTAGA GATGACGGGA CTTCATAAAG ACACTGCTGC TGTTACGCAG 
ATACACTTCC TCTGTGGACA GGGAAGAATA CTTTCTCTTT TGGATGATAA CACGATTCAC 
CTTTGGGAGA TCGTGCAGAG AGAAAACCGA TCACATTTGC TAGAAGTTCA CAGCTTCAAC 
CTGCCTGGCA GGCCGGGCAT CGAGAGCACA AGTGCAACTC GTGTGACGGT CATGCTGCTG 
AATCTGAGCT GTAATCTTCT CGCTCTGGGG ACTGAAGGTG GTGGGGTTCA CTTTCTGGAG 
TTGCCTACCC TCACACTGCT CAACAAGTCT CTGTTTCAGG ACGAGATCAT GCAGAGCGTT 
CCAGAGGAGT ACAAGTGTGG AAAATCATTG GGGCCTGTGG AGTCCCTGCA GGAACATCCG 
AAAGACTCAG ATAAAATCCT CATTGGCTAC AGCAGAGGTC TGGTGGTGCT CTGGGACCTC 
AGCAGTCGGC ATGTGGACAA CCTCTTCCTG GGAAAGCAGC AGCTGGAGAG TTTGGTCTGG 
GAACGGTCCG GGAACAGCTT TGTTAGTTCT CATAGTGATG GCGGATACAT GGTGTGGGCT 
GTCAGCAGCA GCAATCCCTG CACCCATGAC CCCGTCTCAT CCACTATACC ATATGGCCCC 
TTTCCTTGCA AAGCCGTGAA CAAGATCCTG TGGAGGACGA CAGAATCAGG AGCTCCTCTA 
GTGTTATTCA GTGGCGGGAT GCCCAGAGCC AGCTATGGAG ACAGACACTG CCTCACCATC 
CTGCAGGACA GCAGCCACGT CACGCTGGAC TTCACCTCGC GTGTCATCGA CTTCTTCACT 
ATTCACTGCA CGGATACAGA GAAGGACTTT GACGAGCCTT CAGCACTGGT GGTTCTTCTG 
GAGGAGGAGC TGGTGGTGAT TGATCTTCAG ATGCCTGGAT GGCCCACAGT CCCTGCTCCA 
TATCTGGCTC CTCTGCACTC TTCCGCCATC ACCTGCTCCT GCCACATCTC CAATGTGCCT 
CCCAAACTGT GGGAGCGAGT GATCAGTGCT GGCCAACAGC AGTGTCCGCT GCAGAACTAT 
GAGAATTGGC CAATATGTGG AGGGAAAAAC TTAGCGCCTG ATCCAAAACA GAAGGAACTG 
CTGTTAACTG GTCATGAGGA CGGAACAGTG CGTTTCTGGG ATGCGTCAGG AGTGTCACTC 
AAACCTTTAT ACAAACTGAG CACGGCCTCC ATCTTTCAGA CAGACTGCGA GCACAACGAC 
AGCCTGACAC AAGCCGGAGA GGAGGAATGG CCTCCATTCA GAAAGGTGGG CTGCTTTGAC 
CCATACAGTG ATGACCCCAG ACTTGGAATC CAGAAAATCA GCCTCTGTAA ATACAGTGGA 
AAACTGGTGG TGGCTGGAAC TGCTGGACAA GTTATAGTCA TGGTTCTTGG TGATGAGAAG 
TCAGACCATA TGATTGATGT GGCCACAGTG GACCTGCTCC AGGACAGAGA GGGCTTCACA 
TGGAAGGGTC ACGACCGGCT GCCTCCTAAA TCGGGCTCAG TAGTGTTTGC TCCTGGATTC 
CAGCCGGTGG TCCTAGTTCA ATGCCTGCCG CCCGCCGCCG TTACTGCAGT GACCCTGCAT 
GCGGAGTGGA ATCTCATTGC CTTTGGCACT AGTCATGGCT TTGGCCTTTT TGACTACCAC 
CGGCGAAGTC CAGTTCTGGC GAGATGCACG CTGCACCCCA ATGACTCTCT GGCCATGGAG 
GGGCCACTGT CCCGAGTGAA GTCCTTGAAG AAGTCCCTGC GCCAGTCCTT CAGGAGGATC 
CGCAAGAGCA GAGTCTCAGG AAAGAAGCGC GTCGTGGTCA ACAGTCCCAC CAGTAAAGTG 
CAAGAGGCGA ACGCTCATTT GGCAGATCAC GATGCTGAAG TGACACCTGT GCAGAGGAGG 
ATTGAGCCCC GTTCAGCGGA TGACTCTCTG TCGGGAGTGG TGCGATGCCT CTGTTTCGCT 
GACACCTTCC TTAGAGACGG AACACATCAT GGTCCTACAA TGTGGGCCGG CACAAACTCT 
GGTTCAGTGT ACGCTTACGC TCTGGACGTC CCGTCACAGG AGAAGTTCTC TGAGCAGAGT 
GTGGAGGCCC TGCTGGGGAA GGAGATCCAG CTGATGCACA GAGCTCCAGT GGTGTCCATC 
GCTGTGCTGG ACGGCCGAGG AAACCCGCTG CCAGAGCCTT ATGAAGTGTC CAGAGATCTG 
GCCAAAGCCC CCGACATGCA GGGAAGCCAC TCGGTTCTTA TCGCTTCTGA GGAGCAGTTT 
AAGGTTTTCA TGCTGCCAAA AGTAAGTGCG AAGACCAAGT TCAAGCTGAC AGCTCATGAG 
GGCTGTCGGG TGCGTAAGGT GGCACTGGCG AACTTCACCA GCGTGAGCTC TGAAGATTAC 
TCTGAGAATG CTCTGGTGTG CCTTACCAAC ATGGGCGACA TCCACATGTT CAGTGTGCCG 
GCTCTCAGAC CGCAAGTGCG TTATGACTGC GTCCGCAAGG AAGACATCAG TGGCATCGCT 
TCTTGTGTTT TCACCAAGAC TGGACAAGGA TTCTACTTGA TTTCTCCATC GGAGTATGAG 
CGATTCTCTC TGTCGGCTCG TGTGATCACA GAGCCGCTGT GTGGAGTGGA TGTAGAGCGA 
CCTCACGATG TCTCTGCGCA CAGTTCCAGC ACGACGCTTC CACCGCAGGC TAATGGAACA 
CACAAGTCAC AAGCAGAGAG CAAGTCTGCT GAAACTGAAG ATTTGTTGGA GGAAATGCGC 
AGTGTCTTGT CTTCTCCCAT CCTGGATTCT CCAAACAGCA GTGCAGACAT CACTCTGGAC 
ACCACTGGAG ATCTGACTGT GGAGGACGTC AAAGACTTCT TAATGACTGT GGATGAAGCG 
GAGAATAACT TCAGGAACAT AACTGAGGAG GACGGGAGAT CTGCTGGAAT CCTCATCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164199.1
CDS	175..3297
Translation	MMKFRFRRQGNDPHREKIKQDLFAFNKTVEHGFPNQPSALAYDPKLQLMAIGTKSGAIKIYGAPGVEMTGLHKDTAAVTQIHFLCGQGRILSLLDDNTIHLWEIVQRENRSHLLEVHSFNLPGRPGIESTSATRVTVMLLNLSCNLLALGTEGGGVHFLELPTLTLLNKSLFQDEIMQSVPEEYKCGKSLGPVESLQEHPKDSDKILIGYSRGLVVLWDLSSRHVDNLFLGKQQLESLVWERSGNSFVSSHSDGGYMVWAVSSSNPCTHDPVSSTIPYGPFPCKAVNKILWRTTESGAPLVLFSGGMPRASYGDRHCLTILQDSSHVTLDFTSRVIDFFTIHCTDTEKDFDEPSALVVLLEEELVVIDLQMPGWPTVPAPYLAPLHSSAITCSCHISNVPPKLWERVISAGQQQCPLQNYENWPICGGKNLAPDPKQKELLLTGHEDGTVRFWDASGVSLKPLYKLSTASIFQTDCEHNDSLTQAGEEEWPPFRKVGCFDPYSDDPRLGIQKISLCKYSGKLVVAGTAGQVIVMVLGDEKSDHMIDVATVDLLQDREGFTWKGHDRLPPKSGSVVFAPGFQPVVLVQCLPPAAVTAVTLHAEWNLIAFGTSHGFGLFDYHRRSPVLARCTLHPNDSLAMEGPLSRVKSLKKSLRQSFRRIRKSRVSGKKRVVVNSPTSKVQEANAHLADHDAEVTPVQRRIEPRSADDSLSGVVRCLCFADTFLRDGTHHGPTMWAGTNSGSVYAYALDVPSQEKFSEQSVEALLGKEIQLMHRAPVVSIAVLDGRGNPLPEPYEVSRDLAKAPDMQGSHSVLIASEEQFKVFMLPKVSAKTKFKLTAHEGCRVRKVALANFTSVSSEDYSENALVCLTNMGDIHMFSVPALRPQVRYDCVRKEDISGIASCVFTKTGQGFYLISPSEYERFSLSARVITEPLCGVDVERPHDVSAHSSSTTLPPQANGTHKSQAESKSAETEDLLEEMRSVLSSPILDSPNSSADITLDTTGDLTVEDVKDFLMTVDEAENNFRNITEEDGRSAGILIN

Target ORF information:

RefSeq Version	XM_005164142.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lethal giant larvae homolog 1 (Drosophila) (llgl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164142.4

ORF Insert Sequence:

ATGATGAAGT TTAGGTTCAG ACGGCAGGGA AATGACCCTC ATCGTGAAAA AATCAAGCAG 
GATCTGTTTG CATTCAATAA GACAGTTGAA CATGGATTTC CAAACCAGCC AAGTGCTTTG 
GCCTATGATC CCAAACTGCA GCTTATGGCT ATTGGAACTA AATCAGGGGC CATCAAAATA 
TATGGTGCTC CAGGGGTAGA GATGACGGGA CTTCATAAAG ACACTGCTGC TGTTACGCAG 
ATACACTTCC TCTGTGGACA GGGAAGAATA CTTTCTCTTT TGGATGATAA CACGATTCAC 
CTTTGGGAGA TCGTGCAGAG AGAAAACCGA TCACATTTGC TAGAAGTTCA CAGCTTCAAC 
CTGCCTGGCA GGCCGGGCAT CGAGAGCACA AGTGCAACTC GTGTGACGGT CATGCTGCTG 
AATCTGAGCT GTAATCTTCT CGCTCTGGGG ACTGAAGGTG GTGGGGTTCA CTTTCTGGAG 
TTGCCTACCC TCACACTGCT CAACAAGTCT CTGTTTCAGG ACGAGATCAT GCAGAGCGTT 
CCAGAGGAGT ACAAGTGTGG AAAATCATTG GGGCCTGTGG AGTCCCTGCA GGAACATCCG 
AAAGACTCAG ATAAAATCCT CATTGGCTAC AGCAGAGGTC TGGTGGTGCT CTGGGACCTC 
AGCAGTCGGC ATGTGGACAA CCTCTTCCTG GGAAAGCAGC AGCTGGAGAG TTTGGTCTGG 
GAACGGTCCG GGAACAGCTT TGTTAGTTCT CATAGTGATG GCGGATACAT GGTGTGGGCT 
GTCAGCAGCA GCAATCCCTG CACCCATGAC CCCGTCTCAT CCACTATACC ATATGGCCCC 
TTTCCTTGCA AAGCCGTGAA CAAGATCCTG TGGAGGACGA CAGAATCAGG AGCTCCTCTA 
GTGTTATTCA GTGGCGGGAT GCCCAGAGCC AGCTATGGAG ACAGACACTG CCTCACCATC 
CTGCAGGACA GCAGCCACGT CACGCTGGAC TTCACCTCGC GTGTCATCGA CTTCTTCACT 
ATTCACTGCA CGGATACAGA GAAGGACTTT GACGAGCCTT CAGCACTGGT GGTTCTTCTG 
GAGGAGGAGC TGGTGGTGAT TGATCTTCAG ATGCCTGGAT GGCCCACAGT CCCTGCTCCA 
TATCTGGCTC CTCTGCACTC TTCCGCCATC ACCTGCTCCT GCCACATCTC CAATGTGCCT 
CCCAAACTGT GGGAGCGAGT GATCAGTGCT GGCCAACAGC AGTGTCCGCT GCAGAACTAT 
GAGAATTGGC CAATATGTGG AGGGAAAAAC TTAGCGCCTG ATCCAAAACA GAAGGAACTG 
CTGTTAACTG GTCATGAGGA CGGAACAGTG CGTTTCTGGG ATGCGTCAGG AGTGTCACTC 
AAACCTTTAT ACAAACTGAG CACGGCCTCC ATCTTTCAGA CAGACTGCGA GCACAACGAC 
AGCCTGACAC AAGCCGGAGA GGAGGAATGG CCTCCATTCA GAAAGGTGGG CTGCTTTGAC 
CCATACAGTG ATGACCCCAG ACTTGGAATC CAGAAAATCA GCCTCTGTAA ATACAGTGGA 
AAACTGGTGG TGGCTGGAAC TGCTGGACAA GTTATAGTCA TGGTTCTTGG TGATGAGAAG 
TCAGACCATA TGATTGATGT GGCCACAGTG GACCTGCTCC AGGACAGAGA GGGCTTCACA 
TGGAAGGGTC ACGACCGGCT GCCTCCTAAA TCGGGCTCAG TAGTGTTTGC TCCTGGATTC 
CAGCCGGTGG TCCTAGTTCA ATGCCTGCCG CCCGCCGCCG TTACTGCAGT GACCCTGCAT 
GCGGAGTGGA ATCTCATTGC CTTTGGCACT AGTCATGGCT TTGGCCTTTT TGACTACCAC 
CGGCGAAGTC CAGTTCTGGC GAGATGCACG CTGCACCCCA ATGACTCTCT GGCCATGGAG 
GGGCCACTGT CCCGAGTGAA GTCCTTGAAG AAGTCCCTGC GCCAGTCCTT CAGGAGGATC 
CGCAAGAGCA GAGTCTCAGG AAAGAAGCGC GTCGTGGTCA ACAGTCCCAC CAGTAAAGTG 
CAAGAGGCGA ACGCTCATTT GGCAGATCAC GATGCTGAAG TGACACCTGT GCAGAGGAGG 
ATTGAGCCCC GTTCAGCGGA TGACTCTCTG TCGGGAGTGG TGCGATGCCT CTGTTTCGCT 
GACACCTTCC TTAGAGACGG AACACATCAT GGTCCTACAA TGTGGGCCGG CACAAACTCT 
GGTTCAGTGT ACGCTTACGC TCTGGACGTC CCGTCACAGG AGAAGTTCTC TGAGCAGAGT 
GTGGAGGCCC TGCTGGGGAA GGAGATCCAG CTGATGCACA GAGCTCCAGT GGTGTCCATC 
GCTGTGCTGG ACGGCCGAGG AAACCCGCTG CCAGAGCCTT ATGAAGTGTC CAGAGATCTG 
GCCAAAGCCC CCGACATGCA GGGAAGCCAC TCGGTTCTTA TCGCTTCTGA GGAGCAGTTT 
AAGGTTTTCA TGCTGCCAAA AGTAAGTGCG AAGACCAAGT TCAAGCTGAC AGCTCATGAG 
GGCTGTCGGG TGCGTAAGGT GGCACTGGCG AACTTCACCA GCGTGAGCTC TGAAGATTAC 
TCTGAGAATG CTCTGGTGTG CCTTACCAAC ATGGGCGACA TCCACATGTT CAGTGTGCCG 
GCTCTCAGAC CGCAAGTGCG TTATGACTGC GTCCGCAAGG AAGACATCAG TGGCATCGCT 
TCTTGTGTTT TCACCAAGAC TGGACAAGGA TTCTACTTGA TTTCTCCATC GGAGTATGAG 
CGATTCTCTC TGTCGGCTCG TGTGATCACA GAGCCGCTGT GTGGAGTGGA TGTAGAGCGA 
CCTCACGATG TCTCTGCGCA CAGTTCCAGC ACGACGCTTC CACCGCAGGC TAATGGAACA 
CACAAGTCAC AAGCAGAGAG CAAGTCTGCT GAAACTGAAG ATTTGTTGGA GGAAATGCGC 
AGTGTCTTGT CTTCTCCCAT CCTGGATTCT CCAAACAGCA GTGCAGACAT CACTCTGGAC 
ACCACTGGAG ATCTGACTGT GGAGGACGTC AAAGACTTCT TAATGACTGT GGATGAAGCG 
GAGAATAACT TCAGGAACAT AACTGAGGAG GACGGGAGAT CTGCTGGAAT CCTCATCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LLGL1 ( XM_005164142.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005164199.1 Danio rerio lethal giant larvae homolog 1 (Drosophila) (llgl1), transcript variant X2, mRNA.

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CloneID	ODa08599
Clone ID Related Accession (Same CDS sequence)	XM_005164142.4 , XM_005164142.3
Accession Version	XM_005164142.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	lethal(2) giant larvae protein homolog 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164142.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGT TTAGGTTCAG ACGGCAGGGA AATGACCCTC ATCGTGAAAA AATCAAGCAG 
GATCTGTTTG CATTCAATAA GACAGTTGAA CATGGATTTC CAAACCAGCC AAGTGCTTTG 
GCCTATGATC CCAAACTGCA GCTTATGGCT ATTGGAACTA AATCAGGGGC CATCAAAATA 
TATGGTGCTC CAGGGGTAGA GATGACGGGA CTTCATAAAG ACACTGCTGC TGTTACGCAG 
ATACACTTCC TCTGTGGACA GGGAAGAATA CTTTCTCTTT TGGATGATAA CACGATTCAC 
CTTTGGGAGA TCGTGCAGAG AGAAAACCGA TCACATTTGC TAGAAGTTCA CAGCTTCAAC 
CTGCCTGGCA GGCCGGGCAT CGAGAGCACA AGTGCAACTC GTGTGACGGT CATGCTGCTG 
AATCTGAGCT GTAATCTTCT CGCTCTGGGG ACTGAAGGTG GTGGGGTTCA CTTTCTGGAG 
TTGCCTACCC TCACACTGCT CAACAAGTCT CTGTTTCAGG ACGAGATCAT GCAGAGCGTT 
CCAGAGGAGT ACAAGTGTGG AAAATCATTG GGGCCTGTGG AGTCCCTGCA GGAACATCCG 
AAAGACTCAG ATAAAATCCT CATTGGCTAC AGCAGAGGTC TGGTGGTGCT CTGGGACCTC 
AGCAGTCGGC ATGTGGACAA CCTCTTCCTG GGAAAGCAGC AGCTGGAGAG TTTGGTCTGG 
GAACGGTCCG GGAACAGCTT TGTTAGTTCT CATAGTGATG GCGGATACAT GGTGTGGGCT 
GTCAGCAGCA GCAATCCCTG CACCCATGAC CCCGTCTCAT CCACTATACC ATATGGCCCC 
TTTCCTTGCA AAGCCGTGAA CAAGATCCTG TGGAGGACGA CAGAATCAGG AGCTCCTCTA 
GTGTTATTCA GTGGCGGGAT GCCCAGAGCC AGCTATGGAG ACAGACACTG CCTCACCATC 
CTGCAGGACA GCAGCCACGT CACGCTGGAC TTCACCTCGC GTGTCATCGA CTTCTTCACT 
ATTCACTGCA CGGATACAGA GAAGGACTTT GACGAGCCTT CAGCACTGGT GGTTCTTCTG 
GAGGAGGAGC TGGTGGTGAT TGATCTTCAG ATGCCTGGAT GGCCCACAGT CCCTGCTCCA 
TATCTGGCTC CTCTGCACTC TTCCGCCATC ACCTGCTCCT GCCACATCTC CAATGTGCCT 
CCCAAACTGT GGGAGCGAGT GATCAGTGCT GGCCAACAGC AGTGTCCGCT GCAGAACTAT 
GAGAATTGGC CAATATGTGG AGGGAAAAAC TTAGCGCCTG ATCCAAAACA GAAGGAACTG 
CTGTTAACTG GTCATGAGGA CGGAACAGTG CGTTTCTGGG ATGCGTCAGG AGTGTCACTC 
AAACCTTTAT ACAAACTGAG CACGGCCTCC ATCTTTCAGA CAGACTGCGA GCACAACGAC 
AGCCTGACAC AAGCCGGAGA GGAGGAATGG CCTCCATTCA GAAAGGTGGG CTGCTTTGAC 
CCATACAGTG ATGACCCCAG ACTTGGAATC CAGAAAATCA GCCTCTGTAA ATACAGTGGA 
AAACTGGTGG TGGCTGGAAC TGCTGGACAA GTTATAGTCA TGGTTCTTGG TGATGAGAAG 
TCAGACCATA TGATTGATGT GGCCACAGTG GACCTGCTCC AGGACAGAGA GGGCTTCACA 
TGGAAGGGTC ACGACCGGCT GCCTCCTAAA TCGGGCTCAG TAGTGTTTGC TCCTGGATTC 
CAGCCGGTGG TCCTAGTTCA ATGCCTGCCG CCCGCCGCCG TTACTGCAGT GACCCTGCAT 
GCGGAGTGGA ATCTCATTGC CTTTGGCACT AGTCATGGCT TTGGCCTTTT TGACTACCAC 
CGGCGAAGTC CAGTTCTGGC GAGATGCACG CTGCACCCCA ATGACTCTCT GGCCATGGAG 
GGGCCACTGT CCCGAGTGAA GTCCTTGAAG AAGTCCCTGC GCCAGTCCTT CAGGAGGATC 
CGCAAGAGCA GAGTCTCAGG AAAGAAGCGC GTCGTGGTCA ACAGTCCCAC CAGTAAAGTG 
CAAGAGGCGA ACGCTCATTT GGCAGATCAC GATGCTGAAG TGACACCTGT GCAGAGGAGG 
ATTGAGCCCC GTTCAGCGGA TGACTCTCTG TCGGGAGTGG TGCGATGCCT CTGTTTCGCT 
GACACCTTCC TTAGAGACGG AACACATCAT GGTCCTACAA TGTGGGCCGG CACAAACTCT 
GGTTCAGTGT ACGCTTACGC TCTGGACGTC CCGTCACAGG AGAAGTTCTC TGAGCAGAGT 
GTGGAGGCCC TGCTGGGGAA GGAGATCCAG CTGATGCACA GAGCTCCAGT GGTGTCCATC 
GCTGTGCTGG ACGGCCGAGG AAACCCGCTG CCAGAGCCTT ATGAAGTGTC CAGAGATCTG 
GCCAAAGCCC CCGACATGCA GGGAAGCCAC TCGGTTCTTA TCGCTTCTGA GGAGCAGTTT 
AAGGTTTTCA TGCTGCCAAA AGTAAGTGCG AAGACCAAGT TCAAGCTGAC AGCTCATGAG 
GGCTGTCGGG TGCGTAAGGT GGCACTGGCG AACTTCACCA GCGTGAGCTC TGAAGATTAC 
TCTGAGAATG CTCTGGTGTG CCTTACCAAC ATGGGCGACA TCCACATGTT CAGTGTGCCG 
GCTCTCAGAC CGCAAGTGCG TTATGACTGC GTCCGCAAGG AAGACATCAG TGGCATCGCT 
TCTTGTGTTT TCACCAAGAC TGGACAAGGA TTCTACTTGA TTTCTCCATC GGAGTATGAG 
CGATTCTCTC TGTCGGCTCG TGTGATCACA GAGCCGCTGT GTGGAGTGGA TGTAGAGCGA 
CCTCACGATG TCTCTGCGCA CAGTTCCAGC ACGACGCTTC CACCGCAGGC TAATGGAACA 
CACAAGTCAC AAGCAGAGAG CAAGTCTGCT GAAACTGAAG ATTTGTTGGA GGAAATGCGC 
AGTGTCTTGT CTTCTCCCAT CCTGGATTCT CCAAACAGCA GTGCAGACAT CACTCTGGAC 
ACCACTGGAG ATCTGACTGT GGAGGACGTC AAAGACTTCT TAATGACTGT GGATGAAGCG 
GAGAATAACT TCAGGAACAT AACTGAGGAG GACGGGAGAT CTGCTGGAAT CCTCATCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164199.1
CDS	157..3279
Translation	MMKFRFRRQGNDPHREKIKQDLFAFNKTVEHGFPNQPSALAYDPKLQLMAIGTKSGAIKIYGAPGVEMTGLHKDTAAVTQIHFLCGQGRILSLLDDNTIHLWEIVQRENRSHLLEVHSFNLPGRPGIESTSATRVTVMLLNLSCNLLALGTEGGGVHFLELPTLTLLNKSLFQDEIMQSVPEEYKCGKSLGPVESLQEHPKDSDKILIGYSRGLVVLWDLSSRHVDNLFLGKQQLESLVWERSGNSFVSSHSDGGYMVWAVSSSNPCTHDPVSSTIPYGPFPCKAVNKILWRTTESGAPLVLFSGGMPRASYGDRHCLTILQDSSHVTLDFTSRVIDFFTIHCTDTEKDFDEPSALVVLLEEELVVIDLQMPGWPTVPAPYLAPLHSSAITCSCHISNVPPKLWERVISAGQQQCPLQNYENWPICGGKNLAPDPKQKELLLTGHEDGTVRFWDASGVSLKPLYKLSTASIFQTDCEHNDSLTQAGEEEWPPFRKVGCFDPYSDDPRLGIQKISLCKYSGKLVVAGTAGQVIVMVLGDEKSDHMIDVATVDLLQDREGFTWKGHDRLPPKSGSVVFAPGFQPVVLVQCLPPAAVTAVTLHAEWNLIAFGTSHGFGLFDYHRRSPVLARCTLHPNDSLAMEGPLSRVKSLKKSLRQSFRRIRKSRVSGKKRVVVNSPTSKVQEANAHLADHDAEVTPVQRRIEPRSADDSLSGVVRCLCFADTFLRDGTHHGPTMWAGTNSGSVYAYALDVPSQEKFSEQSVEALLGKEIQLMHRAPVVSIAVLDGRGNPLPEPYEVSRDLAKAPDMQGSHSVLIASEEQFKVFMLPKVSAKTKFKLTAHEGCRVRKVALANFTSVSSEDYSENALVCLTNMGDIHMFSVPALRPQVRYDCVRKEDISGIASCVFTKTGQGFYLISPSEYERFSLSARVITEPLCGVDVERPHDVSAHSSSTTLPPQANGTHKSQAESKSAETEDLLEEMRSVLSSPILDSPNSSADITLDTTGDLTVEDVKDFLMTVDEAENNFRNITEEDGRSAGILIN

Target ORF information:

RefSeq Version	XM_005164142.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lethal giant larvae homolog 1 (Drosophila) (llgl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164142.3

ORF Insert Sequence:

ATGATGAAGT TTAGGTTCAG ACGGCAGGGA AATGACCCTC ATCGTGAAAA AATCAAGCAG 
GATCTGTTTG CATTCAATAA GACAGTTGAA CATGGATTTC CAAACCAGCC AAGTGCTTTG 
GCCTATGATC CCAAACTGCA GCTTATGGCT ATTGGAACTA AATCAGGGGC CATCAAAATA 
TATGGTGCTC CAGGGGTAGA GATGACGGGA CTTCATAAAG ACACTGCTGC TGTTACGCAG 
ATACACTTCC TCTGTGGACA GGGAAGAATA CTTTCTCTTT TGGATGATAA CACGATTCAC 
CTTTGGGAGA TCGTGCAGAG AGAAAACCGA TCACATTTGC TAGAAGTTCA CAGCTTCAAC 
CTGCCTGGCA GGCCGGGCAT CGAGAGCACA AGTGCAACTC GTGTGACGGT CATGCTGCTG 
AATCTGAGCT GTAATCTTCT CGCTCTGGGG ACTGAAGGTG GTGGGGTTCA CTTTCTGGAG 
TTGCCTACCC TCACACTGCT CAACAAGTCT CTGTTTCAGG ACGAGATCAT GCAGAGCGTT 
CCAGAGGAGT ACAAGTGTGG AAAATCATTG GGGCCTGTGG AGTCCCTGCA GGAACATCCG 
AAAGACTCAG ATAAAATCCT CATTGGCTAC AGCAGAGGTC TGGTGGTGCT CTGGGACCTC 
AGCAGTCGGC ATGTGGACAA CCTCTTCCTG GGAAAGCAGC AGCTGGAGAG TTTGGTCTGG 
GAACGGTCCG GGAACAGCTT TGTTAGTTCT CATAGTGATG GCGGATACAT GGTGTGGGCT 
GTCAGCAGCA GCAATCCCTG CACCCATGAC CCCGTCTCAT CCACTATACC ATATGGCCCC 
TTTCCTTGCA AAGCCGTGAA CAAGATCCTG TGGAGGACGA CAGAATCAGG AGCTCCTCTA 
GTGTTATTCA GTGGCGGGAT GCCCAGAGCC AGCTATGGAG ACAGACACTG CCTCACCATC 
CTGCAGGACA GCAGCCACGT CACGCTGGAC TTCACCTCGC GTGTCATCGA CTTCTTCACT 
ATTCACTGCA CGGATACAGA GAAGGACTTT GACGAGCCTT CAGCACTGGT GGTTCTTCTG 
GAGGAGGAGC TGGTGGTGAT TGATCTTCAG ATGCCTGGAT GGCCCACAGT CCCTGCTCCA 
TATCTGGCTC CTCTGCACTC TTCCGCCATC ACCTGCTCCT GCCACATCTC CAATGTGCCT 
CCCAAACTGT GGGAGCGAGT GATCAGTGCT GGCCAACAGC AGTGTCCGCT GCAGAACTAT 
GAGAATTGGC CAATATGTGG AGGGAAAAAC TTAGCGCCTG ATCCAAAACA GAAGGAACTG 
CTGTTAACTG GTCATGAGGA CGGAACAGTG CGTTTCTGGG ATGCGTCAGG AGTGTCACTC 
AAACCTTTAT ACAAACTGAG CACGGCCTCC ATCTTTCAGA CAGACTGCGA GCACAACGAC 
AGCCTGACAC AAGCCGGAGA GGAGGAATGG CCTCCATTCA GAAAGGTGGG CTGCTTTGAC 
CCATACAGTG ATGACCCCAG ACTTGGAATC CAGAAAATCA GCCTCTGTAA ATACAGTGGA 
AAACTGGTGG TGGCTGGAAC TGCTGGACAA GTTATAGTCA TGGTTCTTGG TGATGAGAAG 
TCAGACCATA TGATTGATGT GGCCACAGTG GACCTGCTCC AGGACAGAGA GGGCTTCACA 
TGGAAGGGTC ACGACCGGCT GCCTCCTAAA TCGGGCTCAG TAGTGTTTGC TCCTGGATTC 
CAGCCGGTGG TCCTAGTTCA ATGCCTGCCG CCCGCCGCCG TTACTGCAGT GACCCTGCAT 
GCGGAGTGGA ATCTCATTGC CTTTGGCACT AGTCATGGCT TTGGCCTTTT TGACTACCAC 
CGGCGAAGTC CAGTTCTGGC GAGATGCACG CTGCACCCCA ATGACTCTCT GGCCATGGAG 
GGGCCACTGT CCCGAGTGAA GTCCTTGAAG AAGTCCCTGC GCCAGTCCTT CAGGAGGATC 
CGCAAGAGCA GAGTCTCAGG AAAGAAGCGC GTCGTGGTCA ACAGTCCCAC CAGTAAAGTG 
CAAGAGGCGA ACGCTCATTT GGCAGATCAC GATGCTGAAG TGACACCTGT GCAGAGGAGG 
ATTGAGCCCC GTTCAGCGGA TGACTCTCTG TCGGGAGTGG TGCGATGCCT CTGTTTCGCT 
GACACCTTCC TTAGAGACGG AACACATCAT GGTCCTACAA TGTGGGCCGG CACAAACTCT 
GGTTCAGTGT ACGCTTACGC TCTGGACGTC CCGTCACAGG AGAAGTTCTC TGAGCAGAGT 
GTGGAGGCCC TGCTGGGGAA GGAGATCCAG CTGATGCACA GAGCTCCAGT GGTGTCCATC 
GCTGTGCTGG ACGGCCGAGG AAACCCGCTG CCAGAGCCTT ATGAAGTGTC CAGAGATCTG 
GCCAAAGCCC CCGACATGCA GGGAAGCCAC TCGGTTCTTA TCGCTTCTGA GGAGCAGTTT 
AAGGTTTTCA TGCTGCCAAA AGTAAGTGCG AAGACCAAGT TCAAGCTGAC AGCTCATGAG 
GGCTGTCGGG TGCGTAAGGT GGCACTGGCG AACTTCACCA GCGTGAGCTC TGAAGATTAC 
TCTGAGAATG CTCTGGTGTG CCTTACCAAC ATGGGCGACA TCCACATGTT CAGTGTGCCG 
GCTCTCAGAC CGCAAGTGCG TTATGACTGC GTCCGCAAGG AAGACATCAG TGGCATCGCT 
TCTTGTGTTT TCACCAAGAC TGGACAAGGA TTCTACTTGA TTTCTCCATC GGAGTATGAG 
CGATTCTCTC TGTCGGCTCG TGTGATCACA GAGCCGCTGT GTGGAGTGGA TGTAGAGCGA 
CCTCACGATG TCTCTGCGCA CAGTTCCAGC ACGACGCTTC CACCGCAGGC TAATGGAACA 
CACAAGTCAC AAGCAGAGAG CAAGTCTGCT GAAACTGAAG ATTTGTTGGA GGAAATGCGC 
AGTGTCTTGT CTTCTCCCAT CCTGGATTCT CCAAACAGCA GTGCAGACAT CACTCTGGAC 
ACCACTGGAG ATCTGACTGT GGAGGACGTC AAAGACTTCT TAATGACTGT GGATGAAGCG 
GAGAATAACT TCAGGAACAT AACTGAGGAG GACGGGAGAT CTGCTGGAAT CCTCATCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LLGL1 ( XM_021471471.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021327146.1 Danio rerio lethal giant larvae homolog 1 (Drosophila) (llgl1), transcript variant X3, mRNA.

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CloneID	ODa141598
Clone ID Related Accession (Same CDS sequence)	XM_021471471.1
Accession Version	XM_021471471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	lethal(2) giant larvae protein homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGT TTAGGTTCAG ACGGCAGGGA AATGACCCTC ATCGTGAAAA AATCAAGCAG 
GATCTGTTTG CATTCAATAA GACAGTTGAA CATGGATTTC CAAACCAGCC AAGTGCTTTG 
GCCTATGATC CCAAACTGCA GCTTATGGCT ATTGGAACTA AATCAGGGGC CATCAAAATA 
TATGGTGCTC CAGGGGTAGA GATGACGGGA CTTCATAAAG ACACTGCTGC TGTTACGCAG 
ATACACTTCC TCTGTGGACA GGGAAGAATA CTTTCTCTTT TGGATGATAA CACGATTCAC 
CTTTGGGAGA TCGTGCAGAG AGAAAACCGA TCACATTTGC TAGAAGTTCA CAGCTTCAAC 
CTGCCTGGCA GGCCGGGCAT CGAGAGCACA AGTGCAACTC GTGTGACGGT CATGCTGCTG 
AATCTGAGCT GTAATCTTCT CGCTCTGGGG ACTGAAGGTG GTGGGGTTCA CTTTCTGGAG 
TTGCCTACCC TCACACTGCT CAACAAGTCT CTGTTTCAGG ACGAGATCAT GCAGAGCGTT 
CCAGAGGAGT ACAAGTGTGG AAAATCATTG GGGCCTGTGG AGTCCCTGCA GGAACATCCG 
AAAGACTCAG ATAAAATCCT CATTGGCTAC AGCAGAGGTC TGGTGGTGCT CTGGGACCTC 
AGCAGTCGGC ATGTGGACAA CCTCTTCCTG GGAAAGCAGC TGGAGAGTTT GGTCTGGGAA 
CGGTCCGGGA ACAGCTTTGT TAGTTCTCAT AGTGATGGCG GATACATGGT GTGGGCTGTC 
AGCAGCAGCA ATCCCTGCAC CCATGACCCC GTCTCATCCA CTATACCATA TGGCCCCTTT 
CCTTGCAAAG CCGTGAACAA GATCCTGTGG AGGACGACAG AATCAGGAGC TCCTCTAGTG 
TTATTCAGTG GCGGGATGCC CAGAGCCAGC TATGGAGACA GACACTGCCT CACCATCCTG 
CAGGACAGCA GCCACGTCAC GCTGGACTTC ACCTCGCGTG TCATCGACTT CTTCACTATT 
CACTGCACGG ATACAGAGAA GGACTTTGAC GAGCCTTCAG CACTGGTGGT TCTTCTGGAG 
GAGGAGCTGG TGGTGATTGA TCTTCAGATG CCTGGATGGC CCACAGTCCC TGCTCCATAT 
CTGGCTCCTC TGCACTCTTC CGCCATCACC TGCTCCTGCC ACATCTCCAA TGTGCCTCCC 
AAACTGTGGG AGCGAGTGAT CAGTGCTGGC CAACAGCAGT GTCCGCTGCA GAACTATGAG 
AATTGGCCAA TATGTGGAGG GAAAAACTTA GCGCCTGATC CAAAACAGAA GGAACTGCTG 
TTAACTGGTC ATGAGGACGG AACAGTGCGT TTCTGGGATG CGTCAGGAGT GTCACTCAAA 
CCTTTATACA AACTGAGCAC GGCCTCCATC TTTCAGACAG ACTGCGAGCA CAACGACAGC 
CTGACACAAG CCGGAGAGGA GGAATGGCCT CCATTCAGAA AGGTGGGCTG CTTTGACCCA 
TACAGTGATG ACCCCAGACT TGGAATCCAG AAAATCAGCC TCTGTAAATA CAGTGGAAAA 
CTGGTGGTGG CTGGAACTGC TGGACAAGTT ATAGTCATGG TTCTTGGTGA TGAGAAGTCA 
GACCATATGA TTGATGTGGC CACAGTGGAC CTGCTCCAGG ACAGAGAGGG CTTCACATGG 
AAGGGTCACG ACCGGCTGCC TCCTAAATCG GGCTCAGTAG TGTTTGCTCC TGGATTCCAG 
CCGGTGGTCC TAGTTCAATG CCTGCCGCCC GCCGCCGTTA CTGCAGTGAC CCTGCATGCG 
GAGTGGAATC TCATTGCCTT TGGCACTAGT CATGGCTTTG GCCTTTTTGA CTACCACCGG 
CGAAGTCCAG TTCTGGCGAG ATGCACGCTG CACCCCAATG ACTCTCTGGC CATGGAGGGG 
CCACTGTCCC GAGTGAAGTC CTTGAAGAAG TCCCTGCGCC AGTCCTTCAG GAGGATCCGC 
AAGAGCAGAG TCTCAGGAAA GAAGCGCGTC GTGGTCAACA GTCCCACCAG TAAAGTGCAA 
GAGGCGAACG CTCATTTGGC AGATCACGAT GCTGAAGTGA CACCTGTGCA GAGGAGGATT 
GAGCCCCGTT CAGCGGATGA CTCTCTGTCG GGAGTGGTGC GATGCCTCTG TTTCGCTGAC 
ACCTTCCTTA GAGACGGAAC ACATCATGGT CCTACAATGT GGGCCGGCAC AAACTCTGGT 
TCAGTGTACG CTTACGCTCT GGACGTCCCG TCACAGGAGA AGTTCTCTGA GCAGAGTGTG 
GAGGCCCTGC TGGGGAAGGA GATCCAGCTG ATGCACAGAG CTCCAGTGGT GTCCATCGCT 
GTGCTGGACG GCCGAGGAAA CCCGCTGCCA GAGCCTTATG AAGTGTCCAG AGATCTGGCC 
AAAGCCCCCG ACATGCAGGG AAGCCACTCG GTTCTTATCG CTTCTGAGGA GCAGTTTAAG 
GTTTTCATGC TGCCAAAAGT AAGTGCGAAG ACCAAGTTCA AGCTGACAGC TCATGAGGGC 
TGTCGGGTGC GTAAGGTGGC ACTGGCGAAC TTCACCAGCG TGAGCTCTGA AGATTACTCT 
GAGAATGCTC TGGTGTGCCT TACCAACATG GGCGACATCC ACATGTTCAG TGTGCCGGCT 
CTCAGACCGC AAGTGCGTTA TGACTGCGTC CGCAAGGAAG ACATCAGTGG CATCGCTTCT 
TGTGTTTTCA CCAAGACTGG ACAAGGATTC TACTTGATTT CTCCATCGGA GTATGAGCGA 
TTCTCTCTGT CGGCTCGTGT GATCACAGAG CCGCTGTGTG GAGTGGATGT AGAGCGACCT 
CACGATGTCT CTGCGCACAG CAGTTCCAGC ACGACGCTTC CACCGCAGGC TAATGGAACA 
CACAAGTCAC AAGCAGAGAG CAAGTCTGCT GAAACTGAAG ATTTGTTGGA GGAAATGCGC 
AGTGTCTTGT CTTCTCCCAT CCTGGATTCT CCAAACAGCA GTGCAGACAT CACTCTGGAC 
ACCACTGGAG ATCTGACTGT GGAGGACGTC AAAGACTTCT TAATGACTGT GGATGAAGCG 
GAGAATAACT TCAGGAACAT AACTGAGGAG GACGGGAGAT CTGCTGGAAT CCTCATCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327146.1
CDS	175..3297
Translation	MMKFRFRRQGNDPHREKIKQDLFAFNKTVEHGFPNQPSALAYDPKLQLMAIGTKSGAIKIYGAPGVEMTGLHKDTAAVTQIHFLCGQGRILSLLDDNTIHLWEIVQRENRSHLLEVHSFNLPGRPGIESTSATRVTVMLLNLSCNLLALGTEGGGVHFLELPTLTLLNKSLFQDEIMQSVPEEYKCGKSLGPVESLQEHPKDSDKILIGYSRGLVVLWDLSSRHVDNLFLGKQLESLVWERSGNSFVSSHSDGGYMVWAVSSSNPCTHDPVSSTIPYGPFPCKAVNKILWRTTESGAPLVLFSGGMPRASYGDRHCLTILQDSSHVTLDFTSRVIDFFTIHCTDTEKDFDEPSALVVLLEEELVVIDLQMPGWPTVPAPYLAPLHSSAITCSCHISNVPPKLWERVISAGQQQCPLQNYENWPICGGKNLAPDPKQKELLLTGHEDGTVRFWDASGVSLKPLYKLSTASIFQTDCEHNDSLTQAGEEEWPPFRKVGCFDPYSDDPRLGIQKISLCKYSGKLVVAGTAGQVIVMVLGDEKSDHMIDVATVDLLQDREGFTWKGHDRLPPKSGSVVFAPGFQPVVLVQCLPPAAVTAVTLHAEWNLIAFGTSHGFGLFDYHRRSPVLARCTLHPNDSLAMEGPLSRVKSLKKSLRQSFRRIRKSRVSGKKRVVVNSPTSKVQEANAHLADHDAEVTPVQRRIEPRSADDSLSGVVRCLCFADTFLRDGTHHGPTMWAGTNSGSVYAYALDVPSQEKFSEQSVEALLGKEIQLMHRAPVVSIAVLDGRGNPLPEPYEVSRDLAKAPDMQGSHSVLIASEEQFKVFMLPKVSAKTKFKLTAHEGCRVRKVALANFTSVSSEDYSENALVCLTNMGDIHMFSVPALRPQVRYDCVRKEDISGIASCVFTKTGQGFYLISPSEYERFSLSARVITEPLCGVDVERPHDVSAHSSSSTTLPPQANGTHKSQAESKSAETEDLLEEMRSVLSSPILDSPNSSADITLDTTGDLTVEDVKDFLMTVDEAENNFRNITEEDGRSAGILIN

Target ORF information:

RefSeq Version	XM_021471471.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lethal giant larvae homolog 1 (Drosophila) (llgl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471471.1

ORF Insert Sequence:

ATGATGAAGT TTAGGTTCAG ACGGCAGGGA AATGACCCTC ATCGTGAAAA AATCAAGCAG 
GATCTGTTTG CATTCAATAA GACAGTTGAA CATGGATTTC CAAACCAGCC AAGTGCTTTG 
GCCTATGATC CCAAACTGCA GCTTATGGCT ATTGGAACTA AATCAGGGGC CATCAAAATA 
TATGGTGCTC CAGGGGTAGA GATGACGGGA CTTCATAAAG ACACTGCTGC TGTTACGCAG 
ATACACTTCC TCTGTGGACA GGGAAGAATA CTTTCTCTTT TGGATGATAA CACGATTCAC 
CTTTGGGAGA TCGTGCAGAG AGAAAACCGA TCACATTTGC TAGAAGTTCA CAGCTTCAAC 
CTGCCTGGCA GGCCGGGCAT CGAGAGCACA AGTGCAACTC GTGTGACGGT CATGCTGCTG 
AATCTGAGCT GTAATCTTCT CGCTCTGGGG ACTGAAGGTG GTGGGGTTCA CTTTCTGGAG 
TTGCCTACCC TCACACTGCT CAACAAGTCT CTGTTTCAGG ACGAGATCAT GCAGAGCGTT 
CCAGAGGAGT ACAAGTGTGG AAAATCATTG GGGCCTGTGG AGTCCCTGCA GGAACATCCG 
AAAGACTCAG ATAAAATCCT CATTGGCTAC AGCAGAGGTC TGGTGGTGCT CTGGGACCTC 
AGCAGTCGGC ATGTGGACAA CCTCTTCCTG GGAAAGCAGC TGGAGAGTTT GGTCTGGGAA 
CGGTCCGGGA ACAGCTTTGT TAGTTCTCAT AGTGATGGCG GATACATGGT GTGGGCTGTC 
AGCAGCAGCA ATCCCTGCAC CCATGACCCC GTCTCATCCA CTATACCATA TGGCCCCTTT 
CCTTGCAAAG CCGTGAACAA GATCCTGTGG AGGACGACAG AATCAGGAGC TCCTCTAGTG 
TTATTCAGTG GCGGGATGCC CAGAGCCAGC TATGGAGACA GACACTGCCT CACCATCCTG 
CAGGACAGCA GCCACGTCAC GCTGGACTTC ACCTCGCGTG TCATCGACTT CTTCACTATT 
CACTGCACGG ATACAGAGAA GGACTTTGAC GAGCCTTCAG CACTGGTGGT TCTTCTGGAG 
GAGGAGCTGG TGGTGATTGA TCTTCAGATG CCTGGATGGC CCACAGTCCC TGCTCCATAT 
CTGGCTCCTC TGCACTCTTC CGCCATCACC TGCTCCTGCC ACATCTCCAA TGTGCCTCCC 
AAACTGTGGG AGCGAGTGAT CAGTGCTGGC CAACAGCAGT GTCCGCTGCA GAACTATGAG 
AATTGGCCAA TATGTGGAGG GAAAAACTTA GCGCCTGATC CAAAACAGAA GGAACTGCTG 
TTAACTGGTC ATGAGGACGG AACAGTGCGT TTCTGGGATG CGTCAGGAGT GTCACTCAAA 
CCTTTATACA AACTGAGCAC GGCCTCCATC TTTCAGACAG ACTGCGAGCA CAACGACAGC 
CTGACACAAG CCGGAGAGGA GGAATGGCCT CCATTCAGAA AGGTGGGCTG CTTTGACCCA 
TACAGTGATG ACCCCAGACT TGGAATCCAG AAAATCAGCC TCTGTAAATA CAGTGGAAAA 
CTGGTGGTGG CTGGAACTGC TGGACAAGTT ATAGTCATGG TTCTTGGTGA TGAGAAGTCA 
GACCATATGA TTGATGTGGC CACAGTGGAC CTGCTCCAGG ACAGAGAGGG CTTCACATGG 
AAGGGTCACG ACCGGCTGCC TCCTAAATCG GGCTCAGTAG TGTTTGCTCC TGGATTCCAG 
CCGGTGGTCC TAGTTCAATG CCTGCCGCCC GCCGCCGTTA CTGCAGTGAC CCTGCATGCG 
GAGTGGAATC TCATTGCCTT TGGCACTAGT CATGGCTTTG GCCTTTTTGA CTACCACCGG 
CGAAGTCCAG TTCTGGCGAG ATGCACGCTG CACCCCAATG ACTCTCTGGC CATGGAGGGG 
CCACTGTCCC GAGTGAAGTC CTTGAAGAAG TCCCTGCGCC AGTCCTTCAG GAGGATCCGC 
AAGAGCAGAG TCTCAGGAAA GAAGCGCGTC GTGGTCAACA GTCCCACCAG TAAAGTGCAA 
GAGGCGAACG CTCATTTGGC AGATCACGAT GCTGAAGTGA CACCTGTGCA GAGGAGGATT 
GAGCCCCGTT CAGCGGATGA CTCTCTGTCG GGAGTGGTGC GATGCCTCTG TTTCGCTGAC 
ACCTTCCTTA GAGACGGAAC ACATCATGGT CCTACAATGT GGGCCGGCAC AAACTCTGGT 
TCAGTGTACG CTTACGCTCT GGACGTCCCG TCACAGGAGA AGTTCTCTGA GCAGAGTGTG 
GAGGCCCTGC TGGGGAAGGA GATCCAGCTG ATGCACAGAG CTCCAGTGGT GTCCATCGCT 
GTGCTGGACG GCCGAGGAAA CCCGCTGCCA GAGCCTTATG AAGTGTCCAG AGATCTGGCC 
AAAGCCCCCG ACATGCAGGG AAGCCACTCG GTTCTTATCG CTTCTGAGGA GCAGTTTAAG 
GTTTTCATGC TGCCAAAAGT AAGTGCGAAG ACCAAGTTCA AGCTGACAGC TCATGAGGGC 
TGTCGGGTGC GTAAGGTGGC ACTGGCGAAC TTCACCAGCG TGAGCTCTGA AGATTACTCT 
GAGAATGCTC TGGTGTGCCT TACCAACATG GGCGACATCC ACATGTTCAG TGTGCCGGCT 
CTCAGACCGC AAGTGCGTTA TGACTGCGTC CGCAAGGAAG ACATCAGTGG CATCGCTTCT 
TGTGTTTTCA CCAAGACTGG ACAAGGATTC TACTTGATTT CTCCATCGGA GTATGAGCGA 
TTCTCTCTGT CGGCTCGTGT GATCACAGAG CCGCTGTGTG GAGTGGATGT AGAGCGACCT 
CACGATGTCT CTGCGCACAG CAGTTCCAGC ACGACGCTTC CACCGCAGGC TAATGGAACA 
CACAAGTCAC AAGCAGAGAG CAAGTCTGCT GAAACTGAAG ATTTGTTGGA GGAAATGCGC 
AGTGTCTTGT CTTCTCCCAT CCTGGATTCT CCAAACAGCA GTGCAGACAT CACTCTGGAC 
ACCACTGGAG ATCTGACTGT GGAGGACGTC AAAGACTTCT TAATGACTGT GGATGAAGCG 
GAGAATAACT TCAGGAACAT AACTGAGGAG GACGGGAGAT CTGCTGGAAT CCTCATCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

aPKC regulates apical localization of Lgl to restrict elongation of microridges in developing zebrafish epidermis.
Nature communications711643(2016 06)
Raman R,Damle I,Rote R,Banerjee S,Dingare C,Sonawane M

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Scube activity is necessary for Hedgehog signal transduction in vivo.
Developmental biology368(2)193-202(2012 Aug)
Johnson JL,Hall TE,Dyson JM,Sonntag C,Ayers K,Berger S,Gautier P,Mitchell C,Hollway GE,Currie PD

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LLGL1 cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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