acsf2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	acsf2
Entrez Gene ID	751707
Full Name	acyl-CoA synthetase family member 2
Synonyms	im:7145554,wu:fi49b09,zgc:152887
General protein information	Preferred Names acyl-CoA synthetase family member 2 Names acyl-CoA synthetase family member 2, mitochondrial
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001139438.1	NP_001132910.1	medium-chain acyl-CoA ligase ACSF2, mitochondrial precursor
NM_001045419.2	NP_001038884.2	medium-chain acyl-CoA ligase ACSF2, mitochondrial precursor

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	acs-2	NP_506869.1
Mus musculus (house mouse)	Acsf2	NP_722502.1
Homo sapiens (human)	ACSF2	NP_001275897.1
Macaca mulatta (Rhesus monkey)	ACSF2	XP_001097818.1
Gallus gallus (chicken)	ACSF2	XP_420105.3
Bos taurus (cattle)	ACSF2	NP_001071580.1
Rattus norvegicus (Norway rat)	Acsf2	NP_001030123.1
Danio rerio (zebrafish)	acsf2	NP_001132910.1
Magnaporthe oryzae (rice blast fungus)	MGG_08288	XP_003715794.1
Neurospora crassa	NCU06063	XP_959826.1
Canis lupus familiaris (dog)	ACSF2	XP_005625041.1
Drosophila melanogaster (fruit fly)	CG12512	NP_608924.1
Caenorhabditis elegans (roundworm)	acs-1	NP_001023938.1
Xenopus tropicalis (tropical clawed frog)	acsf2	XP_002939621.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008557	XP_314666.4
Pan troglodytes (chimpanzee)	ACSF2	XP_003315663.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.
Gistelinck C, Gioia R, Gagliardi A, Tonelli F, Marchese L, Bianchi L, Landi C, Bini L, Huysseune A, Witten PE, Staes A, Gevaert K, De Rocker N, Menten B, Malfait F, Leikin S, Carra S, Tenni R, Rossi A, De Paepe A, Coucke P, Willaert A, Forlino A
Scientific reports621540(2016 Feb)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Evidence for 26 distinct acyl-coenzyme A synthetase genes in the human genome.
Watkins PA, Maiguel D, Jia Z, Pevsner J
Journal of lipid research48(12)2736-50(2007 Dec)

Many genes in fish have species-specific asymmetric rates of molecular evolution.
Steinke D, Salzburger W, Braasch I, Meyer A
BMC genomics720(2006 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following acsf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the acsf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38764	NM_001045419.2 Latest version!	Danio rerio acyl-CoA synthetase family member 2 (acsf2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa38764	NM_001139438.1 Latest version!	Danio rerio acyl-CoA synthetase family member 2 (acsf2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

acsf2 ( NM_001139438.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001132910.1 Danio rerio acyl-CoA synthetase family member 2 (acsf2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa38764
Clone ID Related Accession (Same CDS sequence)	NM_001139438.1 , NM_001045419.2
Accession Version	NM_001139438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Danio rerio(zebrafish)
Product	medium-chain acyl-CoA ligase ACSF2, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH554699.1, BG737757.1, CF348078.1, DT069701.1 and EH575812.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. ##Evidence-Data-START## CDS exon combination :: BC122098.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTTCTA AGATTCTTCT GACTAATCTA CGAACATCTG CGAGCTTCTG CAAAACCCTG 
AAGTTTCCGC AGAGACCCTG GACTCCTTTC ATTGCTTCCC AACAGAGCTG TGCCATTCAT 
GTGGATAATC CACCCAGCAT CCCAACCCTG ACTACAAGCT ATGTCCATGG CCTCTCTTCC 
CATCCACTGC AGTCCAGTAC TGTGGACCAG TGTCTTCAAG CTACAGTTGA GCGTTACCCA 
GACCGTGAAG CCATGGTTTT TGTGCAGGAT GGAATACGCA AGACCTTTGC TGAGTTTTAC 
CAAGACGTGG AAAAGGCTGC TGCGGGTCTG CTGGCGGCTG GGCTGAAGCG GGGAGATCGA 
TTAGGGATGT GGGGACCAAA TATCTATGAA TGGGTACTGA TGCAGTTTGC CACAGCTAAA 
GCAGGGATCA TTTTGGTTGC GGTGAACCCG GCTTATCAAT TGCAAGAGGT AGAATTTGCT 
CTCAGAAAGG TGCAGTGCAA TGCAGTTGTC TGCCCAACTA AATTCAAATC CCAGCATTAC 
TGTGACATGT TAAAGCAGCT TTGTCCAGAG ATGGAGACTG CTTCACCTGG AGGAATTAAG 
AGTTCAAGAC TTCCAGACTT GCATACTGTG ATCGTGACTG ACAGTCAGCA GCCAGGATCT 
TTCCTTTTGA AAGATCTGAT GCAGGCAGGA AGCAGCCAAC ACTATCAACA ACTTCAAGAC 
CTACAGAAGA AACTTGTTTG CGATGATCCC ATAAACATTC AGTTTACTTC GGGAACTACT 
GGAAAGCCCA AAGGAGCCAC TCTATCTCAT CACAACATCG TGAACAATGC TTATTTCACT 
GGCATGCGCA TTGGCTACAA CTGGAGGAAA AATGTGAGGA TATGCTTGCC GGTGCCCCTG 
TATCACTGTT TTGGCTCAGT GGGTGGTGGT GTAATCATGG CTTTGTATGG CACCACAGTG 
ATTTTTCCAT CAACCGGTTA TGATGGACGT GCCAACCTTC GGGCAATAGA GAAAGAGAAG 
TGTACATTTG TCTACGGTAC CCCCACTATG TATATCGACA TGCTCGGACA GCCAGACCTG 
GCCAAGTTTG ATTTGTCATC AGTTCGGGGT GGTATTGCGG CTGGATCTCC TTGTCCTCCT 
GAGGTCATGC GTAAAATCCT AAATGTCATG GGTATTAAGG AAATGGTGAT TGGCTATGGC 
ACAACTGAAA ACAGCCCTGT GACATTCTGT GGCTTTCCTG TGGACAGTGC AGAGAGAAAG 
ATAGAGACTG TTGGATGTAT TTCCCCACAC ACCGAGGCTA AAGTCGTGGA CCCTACAACA 
GGAGAGATCG TGCCTCTGGG GGCTCAGGGG GAGCTGATGA TCAGAGGGTA TTGTGTGATG 
CTGGAGTACT GGCAGGATGA GGAGAAGACC CGTGAGTGCA TCACGAAAGA CCGCTGGTAC 
AAAACTGGTG ATATTGCCAG TTTGGACCAG TTTGCATACT GCAAAATAGA GGGGCGCATC 
AAAGATTTGA TCATCCGAGG AGGAGAAAAC ATCTATCCTG CTGAAATTGA GCAGTTTCTG 
CACACACATC CCAAAATTCT AGAAGCACAG GTGGTTGGTG TGAAGGATGA GAGGATGGGA 
GAGGAAGTAT GTGCTTGTAT TCGACTTAAG GAGGGTCAGG AATGCACTGT AGAAGAGATT 
AAGGCTTACT GCAAAGGAAA GATTGCCCAT TACAAGGTGC CGCGCTATAT ATTGTTTGTT 
CAGGATTATC CTCTGACAAT TACAGGCAAG ATCCAGAAGC ACAAACTGCG CGAGCGGACC 
GAAAAACAGC TCGGACTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001132910.1
CDS	358..2178
Translation	MSSKILLTNLRTSASFCKTLKFPQRPWTPFIASQQSCAIHVDNPPSIPTLTTSYVHGLSSHPLQSSTVDQCLQATVERYPDREAMVFVQDGIRKTFAEFYQDVEKAAAGLLAAGLKRGDRLGMWGPNIYEWVLMQFATAKAGIILVAVNPAYQLQEVEFALRKVQCNAVVCPTKFKSQHYCDMLKQLCPEMETASPGGIKSSRLPDLHTVIVTDSQQPGSFLLKDLMQAGSSQHYQQLQDLQKKLVCDDPINIQFTSGTTGKPKGATLSHHNIVNNAYFTGMRIGYNWRKNVRICLPVPLYHCFGSVGGGVIMALYGTTVIFPSTGYDGRANLRAIEKEKCTFVYGTPTMYIDMLGQPDLAKFDLSSVRGGIAAGSPCPPEVMRKILNVMGIKEMVIGYGTTENSPVTFCGFPVDSAERKIETVGCISPHTEAKVVDPTTGEIVPLGAQGELMIRGYCVMLEYWQDEEKTRECITKDRWYKTGDIASLDQFAYCKIEGRIKDLIIRGGENIYPAEIEQFLHTHPKILEAQVVGVKDERMGEEVCACIRLKEGQECTVEEIKAYCKGKIAHYKVPRYILFVQDYPLTITGKIQKHKLRERTEKQLGL

Target ORF information:

RefSeq Version	NM_001139438.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acyl-CoA synthetase family member 2 (acsf2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001139438.1

ORF Insert Sequence:

ATGTCTTCTA AGATTCTTCT GACTAATCTA CGAACATCTG CGAGCTTCTG CAAAACCCTG 
AAGTTTCCGC AGAGACCCTG GACTCCTTTC ATTGCTTCCC AACAGAGCTG TGCCATTCAT 
GTGGATAATC CACCCAGCAT CCCAACCCTG ACTACAAGCT ATGTCCATGG CCTCTCTTCC 
CATCCACTGC AGTCCAGTAC TGTGGACCAG TGTCTTCAAG CTACAGTTGA GCGTTACCCA 
GACCGTGAAG CCATGGTTTT TGTGCAGGAT GGAATACGCA AGACCTTTGC TGAGTTTTAC 
CAAGACGTGG AAAAGGCTGC TGCGGGTCTG CTGGCGGCTG GGCTGAAGCG GGGAGATCGA 
TTAGGGATGT GGGGACCAAA TATCTATGAA TGGGTACTGA TGCAGTTTGC CACAGCTAAA 
GCAGGGATCA TTTTGGTTGC GGTGAACCCG GCTTATCAAT TGCAAGAGGT AGAATTTGCT 
CTCAGAAAGG TGCAGTGCAA TGCAGTTGTC TGCCCAACTA AATTCAAATC CCAGCATTAC 
TGTGACATGT TAAAGCAGCT TTGTCCAGAG ATGGAGACTG CTTCACCTGG AGGAATTAAG 
AGTTCAAGAC TTCCAGACTT GCATACTGTG ATCGTGACTG ACAGTCAGCA GCCAGGATCT 
TTCCTTTTGA AAGATCTGAT GCAGGCAGGA AGCAGCCAAC ACTATCAACA ACTTCAAGAC 
CTACAGAAGA AACTTGTTTG CGATGATCCC ATAAACATTC AGTTTACTTC GGGAACTACT 
GGAAAGCCCA AAGGAGCCAC TCTATCTCAT CACAACATCG TGAACAATGC TTATTTCACT 
GGCATGCGCA TTGGCTACAA CTGGAGGAAA AATGTGAGGA TATGCTTGCC GGTGCCCCTG 
TATCACTGTT TTGGCTCAGT GGGTGGTGGT GTAATCATGG CTTTGTATGG CACCACAGTG 
ATTTTTCCAT CAACCGGTTA TGATGGACGT GCCAACCTTC GGGCAATAGA GAAAGAGAAG 
TGTACATTTG TCTACGGTAC CCCCACTATG TATATCGACA TGCTCGGACA GCCAGACCTG 
GCCAAGTTTG ATTTGTCATC AGTTCGGGGT GGTATTGCGG CTGGATCTCC TTGTCCTCCT 
GAGGTCATGC GTAAAATCCT AAATGTCATG GGTATTAAGG AAATGGTGAT TGGCTATGGC 
ACAACTGAAA ACAGCCCTGT GACATTCTGT GGCTTTCCTG TGGACAGTGC AGAGAGAAAG 
ATAGAGACTG TTGGATGTAT TTCCCCACAC ACCGAGGCTA AAGTCGTGGA CCCTACAACA 
GGAGAGATCG TGCCTCTGGG GGCTCAGGGG GAGCTGATGA TCAGAGGGTA TTGTGTGATG 
CTGGAGTACT GGCAGGATGA GGAGAAGACC CGTGAGTGCA TCACGAAAGA CCGCTGGTAC 
AAAACTGGTG ATATTGCCAG TTTGGACCAG TTTGCATACT GCAAAATAGA GGGGCGCATC 
AAAGATTTGA TCATCCGAGG AGGAGAAAAC ATCTATCCTG CTGAAATTGA GCAGTTTCTG 
CACACACATC CCAAAATTCT AGAAGCACAG GTGGTTGGTG TGAAGGATGA GAGGATGGGA 
GAGGAAGTAT GTGCTTGTAT TCGACTTAAG GAGGGTCAGG AATGCACTGT AGAAGAGATT 
AAGGCTTACT GCAAAGGAAA GATTGCCCAT TACAAGGTGC CGCGCTATAT ATTGTTTGTT 
CAGGATTATC CTCTGACAAT TACAGGCAAG ATCCAGAAGC ACAAACTGCG CGAGCGGACC 
GAAAAACAGC TCGGACTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acsf2 ( NM_001045419.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038884.2 Danio rerio acyl-CoA synthetase family member 2 (acsf2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa38764
Clone ID Related Accession (Same CDS sequence)	NM_001139438.1 , NM_001045419.2
Accession Version	NM_001045419.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Danio rerio(zebrafish)
Product	medium-chain acyl-CoA ligase ACSF2, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX324231.9, BG737757.1, CF348078.1, DT069701.1 and EH575812.1. On Dec 20, 2008 this sequence version replaced NM_001045419.1. Transcript Variant: This variant (1) represents the shorter transcript. Variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BC122098.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTTCTA AGATTCTTCT GACTAATCTA CGAACATCTG CGAGCTTCTG CAAAACCCTG 
AAGTTTCCGC AGAGACCCTG GACTCCTTTC ATTGCTTCCC AACAGAGCTG TGCCATTCAT 
GTGGATAATC CACCCAGCAT CCCAACCCTG ACTACAAGCT ATGTCCATGG CCTCTCTTCC 
CATCCACTGC AGTCCAGTAC TGTGGACCAG TGTCTTCAAG CTACAGTTGA GCGTTACCCA 
GACCGTGAAG CCATGGTTTT TGTGCAGGAT GGAATACGCA AGACCTTTGC TGAGTTTTAC 
CAAGACGTGG AAAAGGCTGC TGCGGGTCTG CTGGCGGCTG GGCTGAAGCG GGGAGATCGA 
TTAGGGATGT GGGGACCAAA TATCTATGAA TGGGTACTGA TGCAGTTTGC CACAGCTAAA 
GCAGGGATCA TTTTGGTTGC GGTGAACCCG GCTTATCAAT TGCAAGAGGT AGAATTTGCT 
CTCAGAAAGG TGCAGTGCAA TGCAGTTGTC TGCCCAACTA AATTCAAATC CCAGCATTAC 
TGTGACATGT TAAAGCAGCT TTGTCCAGAG ATGGAGACTG CTTCACCTGG AGGAATTAAG 
AGTTCAAGAC TTCCAGACTT GCATACTGTG ATCGTGACTG ACAGTCAGCA GCCAGGATCT 
TTCCTTTTGA AAGATCTGAT GCAGGCAGGA AGCAGCCAAC ACTATCAACA ACTTCAAGAC 
CTACAGAAGA AACTTGTTTG CGATGATCCC ATAAACATTC AGTTTACTTC GGGAACTACT 
GGAAAGCCCA AAGGAGCCAC TCTATCTCAT CACAACATCG TGAACAATGC TTATTTCACT 
GGCATGCGCA TTGGCTACAA CTGGAGGAAA AATGTGAGGA TATGCTTGCC GGTGCCCCTG 
TATCACTGTT TTGGCTCAGT GGGTGGTGGT GTAATCATGG CTTTGTATGG CACCACAGTG 
ATTTTTCCAT CAACCGGTTA TGATGGACGT GCCAACCTTC GGGCAATAGA GAAAGAGAAG 
TGTACATTTG TCTACGGTAC CCCCACTATG TATATCGACA TGCTCGGACA GCCAGACCTG 
GCCAAGTTTG ATTTGTCATC AGTTCGGGGT GGTATTGCGG CTGGATCTCC TTGTCCTCCT 
GAGGTCATGC GTAAAATCCT AAATGTCATG GGTATTAAGG AAATGGTGAT TGGCTATGGC 
ACAACTGAAA ACAGCCCTGT GACATTCTGT GGCTTTCCTG TGGACAGTGC AGAGAGAAAG 
ATAGAGACTG TTGGATGTAT TTCCCCACAC ACCGAGGCTA AAGTCGTGGA CCCTACAACA 
GGAGAGATCG TGCCTCTGGG GGCTCAGGGG GAGCTGATGA TCAGAGGGTA TTGTGTGATG 
CTGGAGTACT GGCAGGATGA GGAGAAGACC CGTGAGTGCA TCACGAAAGA CCGCTGGTAC 
AAAACTGGTG ATATTGCCAG TTTGGACCAG TTTGCATACT GCAAAATAGA GGGGCGCATC 
AAAGATTTGA TCATCCGAGG AGGAGAAAAC ATCTATCCTG CTGAAATTGA GCAGTTTCTG 
CACACACATC CCAAAATTCT AGAAGCACAG GTGGTTGGTG TGAAGGATGA GAGGATGGGA 
GAGGAAGTAT GTGCTTGTAT TCGACTTAAG GAGGGTCAGG AATGCACTGT AGAAGAGATT 
AAGGCTTACT GCAAAGGAAA GATTGCCCAT TACAAGGTGC CGCGCTATAT ATTGTTTGTT 
CAGGATTATC CTCTGACAAT TACAGGCAAG ATCCAGAAGC ACAAACTGCG CGAGCGGACC 
GAAAAACAGC TCGGACTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038884.2
CDS	198..2018
Translation	MSSKILLTNLRTSASFCKTLKFPQRPWTPFIASQQSCAIHVDNPPSIPTLTTSYVHGLSSHPLQSSTVDQCLQATVERYPDREAMVFVQDGIRKTFAEFYQDVEKAAAGLLAAGLKRGDRLGMWGPNIYEWVLMQFATAKAGIILVAVNPAYQLQEVEFALRKVQCNAVVCPTKFKSQHYCDMLKQLCPEMETASPGGIKSSRLPDLHTVIVTDSQQPGSFLLKDLMQAGSSQHYQQLQDLQKKLVCDDPINIQFTSGTTGKPKGATLSHHNIVNNAYFTGMRIGYNWRKNVRICLPVPLYHCFGSVGGGVIMALYGTTVIFPSTGYDGRANLRAIEKEKCTFVYGTPTMYIDMLGQPDLAKFDLSSVRGGIAAGSPCPPEVMRKILNVMGIKEMVIGYGTTENSPVTFCGFPVDSAERKIETVGCISPHTEAKVVDPTTGEIVPLGAQGELMIRGYCVMLEYWQDEEKTRECITKDRWYKTGDIASLDQFAYCKIEGRIKDLIIRGGENIYPAEIEQFLHTHPKILEAQVVGVKDERMGEEVCACIRLKEGQECTVEEIKAYCKGKIAHYKVPRYILFVQDYPLTITGKIQKHKLRERTEKQLGL

Target ORF information:

RefSeq Version	NM_001045419.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acyl-CoA synthetase family member 2 (acsf2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045419.2

ORF Insert Sequence:

ATGTCTTCTA AGATTCTTCT GACTAATCTA CGAACATCTG CGAGCTTCTG CAAAACCCTG 
AAGTTTCCGC AGAGACCCTG GACTCCTTTC ATTGCTTCCC AACAGAGCTG TGCCATTCAT 
GTGGATAATC CACCCAGCAT CCCAACCCTG ACTACAAGCT ATGTCCATGG CCTCTCTTCC 
CATCCACTGC AGTCCAGTAC TGTGGACCAG TGTCTTCAAG CTACAGTTGA GCGTTACCCA 
GACCGTGAAG CCATGGTTTT TGTGCAGGAT GGAATACGCA AGACCTTTGC TGAGTTTTAC 
CAAGACGTGG AAAAGGCTGC TGCGGGTCTG CTGGCGGCTG GGCTGAAGCG GGGAGATCGA 
TTAGGGATGT GGGGACCAAA TATCTATGAA TGGGTACTGA TGCAGTTTGC CACAGCTAAA 
GCAGGGATCA TTTTGGTTGC GGTGAACCCG GCTTATCAAT TGCAAGAGGT AGAATTTGCT 
CTCAGAAAGG TGCAGTGCAA TGCAGTTGTC TGCCCAACTA AATTCAAATC CCAGCATTAC 
TGTGACATGT TAAAGCAGCT TTGTCCAGAG ATGGAGACTG CTTCACCTGG AGGAATTAAG 
AGTTCAAGAC TTCCAGACTT GCATACTGTG ATCGTGACTG ACAGTCAGCA GCCAGGATCT 
TTCCTTTTGA AAGATCTGAT GCAGGCAGGA AGCAGCCAAC ACTATCAACA ACTTCAAGAC 
CTACAGAAGA AACTTGTTTG CGATGATCCC ATAAACATTC AGTTTACTTC GGGAACTACT 
GGAAAGCCCA AAGGAGCCAC TCTATCTCAT CACAACATCG TGAACAATGC TTATTTCACT 
GGCATGCGCA TTGGCTACAA CTGGAGGAAA AATGTGAGGA TATGCTTGCC GGTGCCCCTG 
TATCACTGTT TTGGCTCAGT GGGTGGTGGT GTAATCATGG CTTTGTATGG CACCACAGTG 
ATTTTTCCAT CAACCGGTTA TGATGGACGT GCCAACCTTC GGGCAATAGA GAAAGAGAAG 
TGTACATTTG TCTACGGTAC CCCCACTATG TATATCGACA TGCTCGGACA GCCAGACCTG 
GCCAAGTTTG ATTTGTCATC AGTTCGGGGT GGTATTGCGG CTGGATCTCC TTGTCCTCCT 
GAGGTCATGC GTAAAATCCT AAATGTCATG GGTATTAAGG AAATGGTGAT TGGCTATGGC 
ACAACTGAAA ACAGCCCTGT GACATTCTGT GGCTTTCCTG TGGACAGTGC AGAGAGAAAG 
ATAGAGACTG TTGGATGTAT TTCCCCACAC ACCGAGGCTA AAGTCGTGGA CCCTACAACA 
GGAGAGATCG TGCCTCTGGG GGCTCAGGGG GAGCTGATGA TCAGAGGGTA TTGTGTGATG 
CTGGAGTACT GGCAGGATGA GGAGAAGACC CGTGAGTGCA TCACGAAAGA CCGCTGGTAC 
AAAACTGGTG ATATTGCCAG TTTGGACCAG TTTGCATACT GCAAAATAGA GGGGCGCATC 
AAAGATTTGA TCATCCGAGG AGGAGAAAAC ATCTATCCTG CTGAAATTGA GCAGTTTCTG 
CACACACATC CCAAAATTCT AGAAGCACAG GTGGTTGGTG TGAAGGATGA GAGGATGGGA 
GAGGAAGTAT GTGCTTGTAT TCGACTTAAG GAGGGTCAGG AATGCACTGT AGAAGAGATT 
AAGGCTTACT GCAAAGGAAA GATTGCCCAT TACAAGGTGC CGCGCTATAT ATTGTTTGTT 
CAGGATTATC CTCTGACAAT TACAGGCAAG ATCCAGAAGC ACAAACTGCG CGAGCGGACC 
GAAAAACAGC TCGGACTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.
Scientific reports621540(2016 Feb)
Gistelinck C,Gioia R,Gagliardi A,Tonelli F,Marchese L,Bianchi L,Landi C,Bini L,Huysseune A,Witten PE,Staes A,Gevaert K,De Rocker N,Menten B,Malfait F,Leikin S,Carra S,Tenni R,Rossi A,De Paepe A,Coucke P,Willaert A,Forlino A

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Evidence for 26 distinct acyl-coenzyme A synthetase genes in the human genome.
Journal of lipid research48(12)2736-50(2007 Dec)
Watkins PA,Maiguel D,Jia Z,Pevsner J

Many genes in fish have species-specific asymmetric rates of molecular evolution.
BMC genomics720(2006 Feb)
Steinke D,Salzburger W,Braasch I,Meyer A

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acsf2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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