mif cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mif
Entrez Gene ID	751093
Full Name	macrophage migration inhibitory factor
General protein information	Preferred Names macrophage migration inhibitory factor Names macrophage migration inhibitory factor
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001043321.1	NP_001036786.1	macrophage migration inhibitory factor

Pathways from BioSystems

Homologies

Homo sapiens (human)	MIF	NP_002406.1
Mus musculus (house mouse)	Mif	NP_034928.1
Xenopus tropicalis (tropical clawed frog)	mif	NP_001107147.1
Bos taurus (cattle)	MIF	NP_001028780.1
Rattus norvegicus (Norway rat)	Mif	NP_112313.1
Danio rerio (zebrafish)	mif	NP_001036786.1
Gallus gallus (chicken)	MIF	XP_003642282.1
Macaca mulatta (Rhesus monkey)	MIF	NP_001028087.1

Gene Ontology
Bibliography

Related articles in PubMed

The role of jab1, a putative downstream effector of the neurotrophic cytokine macrophage migration inhibitory factor (MIF) in zebrafish inner ear hair cell development.
Weber LJ, Marcy HK, Shen YC, Tomkovich SE, Brooks KM, Hilk KE, Barald KF
Experimental neurology301(Pt B)100-109(2018 03)

Tumor initiating cells induce Cxcr4-mediated infiltration of pro-tumoral macrophages into the brain.
Chia K, Mazzolini J, Mione M, Sieger D
eLife7(2018 02)

Inhibited Carnitine Synthesis Causes Systemic Alteration of Nutrient Metabolism in Zebrafish.
Li JM, Li LY, Qin X, Degrace P, Demizieux L, Limbu SM, Wang X, Zhang ML, Li DL, Du ZY
Frontiers in physiology9509(2018)

Histological and transcriptomic effects of 17α-methyltestosterone on zebrafish gonad development.
Lee SLJ, Horsfield JA, Black MA, Rutherford K, Fisher A, Gemmell NJ
BMC genomics18(1)557(2017 07)

Functional characterization of zebrafish orthologs of the human Beta 3-Glucosyltransferase B3GLCT gene mutated in Peters Plus Syndrome.
Weh E, Takeuchi H, Muheisen S, Haltiwanger RS, Semina EV
PloS one12(9)e0184903(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mif gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mif cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00977	NM_001043321.1 Latest version!	Danio rerio macrophage migration inhibitory factor (mif), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mif ( NM_001043321.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001036786.1 Danio rerio macrophage migration inhibitory factor (mif), mRNA.

Price & Availability↑

CloneID	ODa00977
Clone ID Related Accession (Same CDS sequence)	NM_001043321.1
Accession Version	NM_001043321.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	macrophage migration inhibitory factor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ639953.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ639953.1, CF549588.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCCGATGT TTGTAGTGAA CACAAATGTT GCTAAAGACT CGGTTCCGGC GGAGCTGCTG 
TCGGAGGCCA CGCAGGAGCT CGCCAAAGCC ATGGGCAAAC CCCAGCAGTA CATCGCCGTA 
CAGGTTGTGC CGGATCAGAT GATGATGTTC GGGGGAAAAG GAGACCCGTG TGCGCTCTGC 
TCGCTCACCA GCATCGGGAA GATCAGCGGC GCGCAGAATA AACAATACTC CAAACTCCTC 
ATGGGTTTAC TCAACAAACA CCTCGGCGTT TCTGCTGACA GGATCTATAT AAACTTTGTT 
GACATGGATC CAGCCAATGT GGCCTGGAAC AACAGCACCT TTGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036786.1
CDS	123..470
Translation	MPMFVVNTNVAKDSVPAELLSEATQELAKAMGKPQQYIAVQVVPDQMMMFGGKGDPCALCSLTSIGKISGAQNKQYSKLLMGLLNKHLGVSADRIYINFVDMDPANVAWNNSTFG

Target ORF information:

RefSeq Version	NM_001043321.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio macrophage migration inhibitory factor (mif), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043321.1

ORF Insert Sequence:

ATGCCGATGT TTGTAGTGAA CACAAATGTT GCTAAAGACT CGGTTCCGGC GGAGCTGCTG 
TCGGAGGCCA CGCAGGAGCT CGCCAAAGCC ATGGGCAAAC CCCAGCAGTA CATCGCCGTA 
CAGGTTGTGC CGGATCAGAT GATGATGTTC GGGGGAAAAG GAGACCCGTG TGCGCTCTGC 
TCGCTCACCA GCATCGGGAA GATCAGCGGC GCGCAGAATA AACAATACTC CAAACTCCTC 
ATGGGTTTAC TCAACAAACA CCTCGGCGTT TCTGCTGACA GGATCTATAT AAACTTTGTT 
GACATGGATC CAGCCAATGT GGCCTGGAAC AACAGCACCT TTGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The role of jab1, a putative downstream effector of the neurotrophic cytokine macrophage migration inhibitory factor (MIF) in zebrafish inner ear hair cell development.
Experimental neurology301(Pt B)100-109(2018 03)
Weber LJ,Marcy HK,Shen YC,Tomkovich SE,Brooks KM,Hilk KE,Barald KF

Tumor initiating cells induce Cxcr4-mediated infiltration of pro-tumoral macrophages into the brain.
eLife7(2018 02)
Chia K,Mazzolini J,Mione M,Sieger D

Inhibited Carnitine Synthesis Causes Systemic Alteration of Nutrient Metabolism in Zebrafish.
Frontiers in physiology9509(2018)
Li JM,Li LY,Qin X,Degrace P,Demizieux L,Limbu SM,Wang X,Zhang ML,Li DL,Du ZY

Histological and transcriptomic effects of 17α-methyltestosterone on zebrafish gonad development.
BMC genomics18(1)557(2017 07)
Lee SLJ,Horsfield JA,Black MA,Rutherford K,Fisher A,Gemmell NJ

Functional characterization of zebrafish orthologs of the human Beta 3-Glucosyltransferase B3GLCT gene mutated in Peters Plus Syndrome.
PloS one12(9)e0184903(2017)
Weh E,Takeuchi H,Muheisen S,Haltiwanger RS,Semina EV