tbx20 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tbx20
Entrez Gene ID	57936
Full Name	T-box 20
Synonyms	ZF-TBX20,cb826,hrt,whz
General protein information	Preferred Names T-box 20 Names T-box transcription factor TBX20 H15-related T-box transcription factor hrT T-box protein 20
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021466746.1	XP_021322421.1	T-box transcription factor TBX20 isoform X1
NM_131506.2	NP_571581.2	T-box transcription factor TBX20

Pathways from BioSystems

Homologies

Homo sapiens (human)	TBX20	NP_001071121.1
Pan troglodytes (chimpanzee)	TBX20	XP_522453.3
Gallus gallus (chicken)	TBX20	NP_989475.1
Canis lupus familiaris (dog)	TBX20	XP_539513.4
Bos taurus (cattle)	TBX20	NP_001179166.1
Mus musculus (house mouse)	Tbx20	NP_919239.1
Xenopus tropicalis (tropical clawed frog)	tbx20	NP_001030292.1
Macaca mulatta (Rhesus monkey)	TBX20	XP_001105053.2
Danio rerio (zebrafish)	tbx20	NP_571581.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome Sequencing of the Japanese Eel (
Chen W, Bian C, You X, Li J, Ye L, Wen Z, Lv Y, Zhang X, Xu J, Yang S, Gu R, Lin X, Shi Q
Marine drugs17(7)(2019 Jul)

Gene profiling of head mesoderm in early zebrafish development: insights into the evolution of cranial mesoderm.
Wang H, Holland PWH, Takahashi T
EvoDevo1014(2019)

Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish.
Burg L, Palmer N, Kikhi K, Miroshnik ES, Rueckert H, Gaddy E, MacPherson Cunningham C, Mattonet K, Lai SL, Marín-Juez R, Waring RB, Stainier DYR, Balciunas D
PLoS genetics14(11)e1007754(2018 11)

Live imaging reveals a conserved role of fatty acid β-oxidation in early lymphatic development in zebrafish.
Zecchin A, Wong BW, Tembuyser B, Souffreau J, Van Nuffelen A, Wyns S, Vinckier S, Carmeliet P, Dewerchin M
Biochemical and biophysical research communications503(1)26-31(2018 09)

TBX20 Regulates Angiogenesis Through the Prokineticin 2-Prokineticin Receptor 1 Pathway.
Meng S, Gu Q, Yang X, Lv J, Owusu I, Matrone G, Chen K, Cooke JP, Fang L
Circulation138(9)913-928(2018 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tbx20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tbx20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa39338	NM_131506.2 Latest version!	Danio rerio T-box transcription factor 20 (tbx20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa149272	XM_021466746.1 Latest version!	Danio rerio T-box 20 (tbx20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tbx20 ( NM_131506.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571581.2 Danio rerio T-box transcription factor 20 (tbx20), mRNA.

Price & Availability↑

CloneID	ODa39338
Clone ID Related Accession (Same CDS sequence)	NM_131506.2
Accession Version	NM_131506.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576252800000
Organism	Danio rerio(zebrafish)
Product	T-box transcription factor TBX20
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF239664.1. On Jun 18, 2015 this sequence version replaced NM_131506.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF239664.1, AF253325.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505370, SAMEA3505371 [ECO:0000350] ##Evidence-Data-END##

ATGGAGTACA CCTCTTCCCC AAAACCTCAG CTCTCTTCCA GAGCGAACGC TTTCTCCATA 
GCCGCACTCA TGTCAAGTGG GAAAACCAAG GACAAGGAGT CCGAGGAAAA CACCATAAAG 
CCGCTGGAAC AATTCGTGGA AAAGTCGTCA TGCCATCCAA ACCTCGGCGA CCTTCCTCCG 
CTGGAGACCC ACAGCGACTT CAGCAGCGGC GGCGGCACCG GCAGCGGCGC TCCATTATGC 
ACGGAGCCGC TCATCCCGAC GACTCCCGGT GTGCCGAGCG AGGAGATGGC CAAGATTTCC 
TGCAGCCTCG AGACCAAAGA GCTGTGGGAC AAGTTTCACG AACTCGGCAC TGAAATGATT 
ATCACAAAGT CTGGAAGACG AATGTTTCCG ACAATTCGTG TGTCATTTTC CGGAGTGGAC 
CCAGACGCCA AATACATTGT GCTAATGGAC ATAGTTCCGG TGGACAATAA GAGATATCGA 
TACGCCTACC ATAGATCTTC ATGGCTGGTG GCGGGAAAGG CAGATCCACC TTTACCTGCT 
AGGTTATATG TTCATCCAGA TTCGCCGTTC ACAGGAGAAC AGCTGTTGAA ACAAATGGTT 
TCATTCGAAA AAGTGAAGCT TACAAATAAC GAACTGGACC AGCACGGACA TATCATCCTC 
AACTCCATGC ACAAGTACCA ACCCCGAGTT CATATCATCA AGAAGAAAGA TCACACCGCG 
TCTCTGCTAA ATCTGAAGTC TGAAGAGTTT CGCACTTTTG TCTTCACCGA GACGGTTTTC 
ACTGCAGTAA CTGCTTACCA GAACCAGCTG ATAACCAGAC TCAAGATTGA CAGCAACCCG 
TTTGCCAAAG GCTTCAGAGA CTCCTCCAGA CTCACGGATA TTGAGAGGGA AAGTGTTGAG 
AGTTTGATCC ACAAGCACTC TTACGCCCGG TCACCCATCC GGACTTATGC TGGAGATGAA 
GAGACTCTGG GAGAAGAAGG ACATTCAGCA CACAGTAGAG GTTCAGCGTT CACTGCATCT 
GACAACCTCT CCCTCAGCTC TTGGGTGACC ACCACATCTG GTTTCTCTGG CTTTCAGCAC 
CCTCAGTCTC TGTCTGCCAT CGGCACCAGC ACGGCGTCCC TGGCCAGTCC TCTCCCGCAC 
CCCATCCAGG GCTCCCTCCC CCCCTACAGT CGTCTGGGAA TGCCCCTTAC TCCATCCGCC 
CTCGCCAGCT CCATGCAGGC CACCGGACCC ACCTTCCCCT CCTTCCACAT GCCGCGCTAC 
CACCACTACT TCCAGCAAGG GCCCTACGCC GCCATCCAGG GACTGCGGCA CTCTTCAACA 
GTCATGACCC CGTTTGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571581.2
CDS	169..1509
Translation	MEYTSSPKPQLSSRANAFSIAALMSSGKTKDKESEENTIKPLEQFVEKSSCHPNLGDLPPLETHSDFSSGGGTGSGAPLCTEPLIPTTPGVPSEEMAKISCSLETKELWDKFHELGTEMIITKSGRRMFPTIRVSFSGVDPDAKYIVLMDIVPVDNKRYRYAYHRSSWLVAGKADPPLPARLYVHPDSPFTGEQLLKQMVSFEKVKLTNNELDQHGHIILNSMHKYQPRVHIIKKKDHTASLLNLKSEEFRTFVFTETVFTAVTAYQNQLITRLKIDSNPFAKGFRDSSRLTDIERESVESLIHKHSYARSPIRTYAGDEETLGEEGHSAHSRGSAFTASDNLSLSSWVTTTSGFSGFQHPQSLSAIGTSTASLASPLPHPIQGSLPPYSRLGMPLTPSALASSMQATGPTFPSFHMPRYHHYFQQGPYAAIQGLRHSSTVMTPFV

Target ORF information:

RefSeq Version	NM_131506.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio T-box transcription factor 20 (tbx20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131506.2

ORF Insert Sequence:

ATGGAGTACA CCTCTTCCCC AAAACCTCAG CTCTCTTCCA GAGCGAACGC TTTCTCCATA 
GCCGCACTCA TGTCAAGTGG GAAAACCAAG GACAAGGAGT CCGAGGAAAA CACCATAAAG 
CCGCTGGAAC AATTCGTGGA AAAGTCGTCA TGCCATCCAA ACCTCGGCGA CCTTCCTCCG 
CTGGAGACCC ACAGCGACTT CAGCAGCGGC GGCGGCACCG GCAGCGGCGC TCCATTATGC 
ACGGAGCCGC TCATCCCGAC GACTCCCGGT GTGCCGAGCG AGGAGATGGC CAAGATTTCC 
TGCAGCCTCG AGACCAAAGA GCTGTGGGAC AAGTTTCACG AACTCGGCAC TGAAATGATT 
ATCACAAAGT CTGGAAGACG AATGTTTCCG ACAATTCGTG TGTCATTTTC CGGAGTGGAC 
CCAGACGCCA AATACATTGT GCTAATGGAC ATAGTTCCGG TGGACAATAA GAGATATCGA 
TACGCCTACC ATAGATCTTC ATGGCTGGTG GCGGGAAAGG CAGATCCACC TTTACCTGCT 
AGGTTATATG TTCATCCAGA TTCGCCGTTC ACAGGAGAAC AGCTGTTGAA ACAAATGGTT 
TCATTCGAAA AAGTGAAGCT TACAAATAAC GAACTGGACC AGCACGGACA TATCATCCTC 
AACTCCATGC ACAAGTACCA ACCCCGAGTT CATATCATCA AGAAGAAAGA TCACACCGCG 
TCTCTGCTAA ATCTGAAGTC TGAAGAGTTT CGCACTTTTG TCTTCACCGA GACGGTTTTC 
ACTGCAGTAA CTGCTTACCA GAACCAGCTG ATAACCAGAC TCAAGATTGA CAGCAACCCG 
TTTGCCAAAG GCTTCAGAGA CTCCTCCAGA CTCACGGATA TTGAGAGGGA AAGTGTTGAG 
AGTTTGATCC ACAAGCACTC TTACGCCCGG TCACCCATCC GGACTTATGC TGGAGATGAA 
GAGACTCTGG GAGAAGAAGG ACATTCAGCA CACAGTAGAG GTTCAGCGTT CACTGCATCT 
GACAACCTCT CCCTCAGCTC TTGGGTGACC ACCACATCTG GTTTCTCTGG CTTTCAGCAC 
CCTCAGTCTC TGTCTGCCAT CGGCACCAGC ACGGCGTCCC TGGCCAGTCC TCTCCCGCAC 
CCCATCCAGG GCTCCCTCCC CCCCTACAGT CGTCTGGGAA TGCCCCTTAC TCCATCCGCC 
CTCGCCAGCT CCATGCAGGC CACCGGACCC ACCTTCCCCT CCTTCCACAT GCCGCGCTAC 
CACCACTACT TCCAGCAAGG GCCCTACGCC GCCATCCAGG GACTGCGGCA CTCTTCAACA 
GTCATGACCC CGTTTGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbx20 ( XM_021466746.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322421.1 Danio rerio T-box 20 (tbx20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa149272
Clone ID Related Accession (Same CDS sequence)	XM_021466746.1
Accession Version	XM_021466746.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	T-box transcription factor TBX20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTACA CCTCTTCCCC AAAACCTCAG CTCTCTTCCA GAGCGAACGC TTTCTCCATA 
GCCGCACTCA TGTCAAGTGG GAAAACCAAG GACAAGGAGT CCGAGGAAAA CACCATAAAG 
CCGCTGGAAC AATTCGTGGA AAAGTCGTCA TGCCATCCAA ACCTCGGCGA CCTTCCTCCG 
CTGGAGACCC ACAGCGACTT CAGCAGCGGC GGCGGCACCG GCAGCGGCGC TCCATTATGC 
ACGGAGCCGC TCATCCCGAC GACTCCCGGT GTGCCGAGCG AGGAGATGGC CAAGATTTCC 
TGCAGCCTCG AGACCAAAGA GCTGTGGGAC AAGTTTCACG AACTCGGCAC TGAAATGATT 
ATTACAAAGT CTGGAAGACG AATGTTTCCG ACAATTCGTG TGTCATTTTC CGGAGTGGAC 
CCAGACGCCA AATACATTGT GCTAATGGAC ATAGTTCCGG TGGACAATAA GAGATATCGA 
TACGCCTACC ATAGATCTTC ATGGCTGGTG GCGGGAAAGG CAGATCCACC TTTACCTGCT 
AGGTTATATG TTCATCCAGA TTCGCCATTC ACAGGAGAAC AGCTGTTGAA ACAAATGGTT 
TCTTTCGAAA AAGTGAAGCT TACAAATAAC GAACTGGACC AGCACGGACA TATCATCCTC 
AACTCCATGC ACAAGTACCA ACCCCGAGTT CATATCATCA AGAAGAAAGA TCACACCGCG 
TCTCTGCTAA ATCTGAAGTC TGAAGAGTTT CGCACTTTTG TCTTCACCGA GACGGTTTTC 
ACTGCAGTAA CTGCTTACCA GAACCAGCTG ATAACCAGAC TCAAGATTGA CAGCAACCCG 
TTTGCCAAAG GCTTCAGAGA CTCCTCCAGA CTCACGGATA TTGAGAGTAG GGAAAGTGTT 
GAGAGTTTGA TCCACAAGCA CTCTTACGCC CGGTCACCCA TCCGGACTTA TGCTGGAGAT 
GAAGAGACTC TGGGAGAAGA AGGACATTCA GCACACAGTA GAGGTTCAGC GTTCACTGCA 
TCTGACAACC TCTCCCTCAG CTCTTGGGTG ACCACCACAT CTGGTTTCTC TGGCTTTCAG 
CACCCTCAGT CTCTGTCTGC CATCGGCACC AGCACGGCGT CCCTGGCCAG TCCTCTCCCG 
CACCCCATCC AGGGCTCCCT CCCCCCCTAC AGTCGTCTGG GAATGCCCCT TACTCCATCC 
GCCCTCGCCA GCTCCATGCA GGCCACCGGA CCCACCTTCC CCTCCTTCCA CATGCCGCGC 
TACCACCACT ACTTCCAGCA AGGGCCCTAC GCCGCCATCC AGGGACTGCG GCACTCTTCA 
ACAGTCATGA CCCCGTTTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322421.1
CDS	359..1702
Translation	MEYTSSPKPQLSSRANAFSIAALMSSGKTKDKESEENTIKPLEQFVEKSSCHPNLGDLPPLETHSDFSSGGGTGSGAPLCTEPLIPTTPGVPSEEMAKISCSLETKELWDKFHELGTEMIITKSGRRMFPTIRVSFSGVDPDAKYIVLMDIVPVDNKRYRYAYHRSSWLVAGKADPPLPARLYVHPDSPFTGEQLLKQMVSFEKVKLTNNELDQHGHIILNSMHKYQPRVHIIKKKDHTASLLNLKSEEFRTFVFTETVFTAVTAYQNQLITRLKIDSNPFAKGFRDSSRLTDIESRESVESLIHKHSYARSPIRTYAGDEETLGEEGHSAHSRGSAFTASDNLSLSSWVTTTSGFSGFQHPQSLSAIGTSTASLASPLPHPIQGSLPPYSRLGMPLTPSALASSMQATGPTFPSFHMPRYHHYFQQGPYAAIQGLRHSSTVMTPFV

Target ORF information:

RefSeq Version	XM_021466746.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio T-box 20 (tbx20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466746.1

ORF Insert Sequence:

ATGGAGTACA CCTCTTCCCC AAAACCTCAG CTCTCTTCCA GAGCGAACGC TTTCTCCATA 
GCCGCACTCA TGTCAAGTGG GAAAACCAAG GACAAGGAGT CCGAGGAAAA CACCATAAAG 
CCGCTGGAAC AATTCGTGGA AAAGTCGTCA TGCCATCCAA ACCTCGGCGA CCTTCCTCCG 
CTGGAGACCC ACAGCGACTT CAGCAGCGGC GGCGGCACCG GCAGCGGCGC TCCATTATGC 
ACGGAGCCGC TCATCCCGAC GACTCCCGGT GTGCCGAGCG AGGAGATGGC CAAGATTTCC 
TGCAGCCTCG AGACCAAAGA GCTGTGGGAC AAGTTTCACG AACTCGGCAC TGAAATGATT 
ATTACAAAGT CTGGAAGACG AATGTTTCCG ACAATTCGTG TGTCATTTTC CGGAGTGGAC 
CCAGACGCCA AATACATTGT GCTAATGGAC ATAGTTCCGG TGGACAATAA GAGATATCGA 
TACGCCTACC ATAGATCTTC ATGGCTGGTG GCGGGAAAGG CAGATCCACC TTTACCTGCT 
AGGTTATATG TTCATCCAGA TTCGCCATTC ACAGGAGAAC AGCTGTTGAA ACAAATGGTT 
TCTTTCGAAA AAGTGAAGCT TACAAATAAC GAACTGGACC AGCACGGACA TATCATCCTC 
AACTCCATGC ACAAGTACCA ACCCCGAGTT CATATCATCA AGAAGAAAGA TCACACCGCG 
TCTCTGCTAA ATCTGAAGTC TGAAGAGTTT CGCACTTTTG TCTTCACCGA GACGGTTTTC 
ACTGCAGTAA CTGCTTACCA GAACCAGCTG ATAACCAGAC TCAAGATTGA CAGCAACCCG 
TTTGCCAAAG GCTTCAGAGA CTCCTCCAGA CTCACGGATA TTGAGAGTAG GGAAAGTGTT 
GAGAGTTTGA TCCACAAGCA CTCTTACGCC CGGTCACCCA TCCGGACTTA TGCTGGAGAT 
GAAGAGACTC TGGGAGAAGA AGGACATTCA GCACACAGTA GAGGTTCAGC GTTCACTGCA 
TCTGACAACC TCTCCCTCAG CTCTTGGGTG ACCACCACAT CTGGTTTCTC TGGCTTTCAG 
CACCCTCAGT CTCTGTCTGC CATCGGCACC AGCACGGCGT CCCTGGCCAG TCCTCTCCCG 
CACCCCATCC AGGGCTCCCT CCCCCCCTAC AGTCGTCTGG GAATGCCCCT TACTCCATCC 
GCCCTCGCCA GCTCCATGCA GGCCACCGGA CCCACCTTCC CCTCCTTCCA CATGCCGCGC 
TACCACCACT ACTTCCAGCA AGGGCCCTAC GCCGCCATCC AGGGACTGCG GCACTCTTCA 
ACAGTCATGA CCCCGTTTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome Sequencing of the Japanese Eel (
Marine drugs17(7)(2019 Jul)
Chen W,Bian C,You X,Li J,Ye L,Wen Z,Lv Y,Zhang X,Xu J,Yang S,Gu R,Lin X,Shi Q

Gene profiling of head mesoderm in early zebrafish development: insights into the evolution of cranial mesoderm.
EvoDevo1014(2019)
Wang H,Holland PWH,Takahashi T

Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish.
PLoS genetics14(11)e1007754(2018 11)
Burg L,Palmer N,Kikhi K,Miroshnik ES,Rueckert H,Gaddy E,MacPherson Cunningham C,Mattonet K,Lai SL,Marín-Juez R,Waring RB,Stainier DYR,Balciunas D

Live imaging reveals a conserved role of fatty acid β-oxidation in early lymphatic development in zebrafish.
Biochemical and biophysical research communications503(1)26-31(2018 09)
Zecchin A,Wong BW,Tembuyser B,Souffreau J,Van Nuffelen A,Wyns S,Vinckier S,Carmeliet P,Dewerchin M

TBX20 Regulates Angiogenesis Through the Prokineticin 2-Prokineticin Receptor 1 Pathway.
Circulation138(9)913-928(2018 08)
Meng S,Gu Q,Yang X,Lv J,Owusu I,Matrone G,Chen K,Cooke JP,Fang L

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tbx20 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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