bbox1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	bbox1
Entrez Gene ID	572649
Full Name	butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1
Synonyms	wu:fj14g08,zgc:112179
General protein information	Preferred Names butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 Names gamma-butyrobetaine dioxygenase butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1 (gamma-butyrobetaine hydroxylase)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001017717.1	NP_001017717.1	gamma-butyrobetaine dioxygenase
XM_005168903.4	XP_005168960.1	gamma-butyrobetaine dioxygenase isoform X1
XM_017356900.1	XP_017212389.1	gamma-butyrobetaine dioxygenase isoform X2
XM_005168904.3	XP_005168961.1	gamma-butyrobetaine dioxygenase isoform X1
XM_005168903.3	XP_005168960.1	gamma-butyrobetaine dioxygenase isoform X1
XM_005168904.4	XP_005168961.1	gamma-butyrobetaine dioxygenase isoform X1
XM_005168905.3	XP_005168962.1	gamma-butyrobetaine dioxygenase isoform X1
XM_021477587.1	XP_021333262.1	gamma-butyrobetaine dioxygenase isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	BBOX1	XP_534096.2
Rattus norvegicus (Norway rat)	Bbox1	NP_072151.1
Bos taurus (cattle)	BBOX1	NP_001095351.2
Mus musculus (house mouse)	Bbox1	NP_569719.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008246	XP_317222.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012477	XP_307672.4
Homo sapiens (human)	BBOX1	NP_003977.1
Neurospora crassa	NCU06891	XP_959238.1
Xenopus tropicalis (tropical clawed frog)	bbox1	NP_001120574.1
Pan troglodytes (chimpanzee)	BBOX1	XP_001135704.1
Gallus gallus (chicken)	BBOX1	XP_424543.2
Danio rerio (zebrafish)	bbox1	NP_001017717.1
Drosophila melanogaster (fruit fly)	CG5321	NP_572967.2
Drosophila melanogaster (fruit fly)	CG10814	NP_610868.1
Caenorhabditis elegans (roundworm)	gbh-1	NP_496305.1

Gene Ontology
Bibliography

Related articles in PubMed

Inhibited Carnitine Synthesis Causes Systemic Alteration of Nutrient Metabolism in Zebrafish.
Li JM, Li LY, Qin X, Degrace P, Demizieux L, Limbu SM, Wang X, Zhang ML, Li DL, Du ZY
Frontiers in physiology9509(2018)

Systemic regulation of L-carnitine in nutritional metabolism in zebrafish, Danio rerio.
Li JM, Li LY, Qin X, Ning LJ, Lu DL, Li DL, Zhang ML, Wang X, Du ZY
Scientific reports740815(2017 01)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Fibin, a novel secreted lateral plate mesoderm signal, is essential for pectoral fin bud initiation in zebrafish.
Wakahara T, Kusu N, Yamauchi H, Kimura I, Konishi M, Miyake A, Itoh N
Developmental biology303(2)527-35(2007 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following bbox1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bbox1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03403	NM_001017717.1 Latest version!	Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ODa13092	XM_005168903.4 Latest version!	Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa13092	XM_005168904.4 Latest version!	Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa13092	XM_005168905.3 Latest version!	Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa13092	XM_021477587.1 Latest version!	Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa59646	XM_017356900.1 Latest version!	Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
ODa13092	XM_005168903.3	Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa13092	XM_005168904.3	Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bbox1 ( NM_001017717.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001017717.1 Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), mRNA.

Price & Availability↑

CloneID	ODa03403
Clone ID Related Accession (Same CDS sequence)	NM_001017717.1
Accession Version	NM_001017717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1539273600000
Organism	Danio rerio(zebrafish)
Product	gamma-butyrobetaine dioxygenase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC093246.1. On Oct 31, 2007 this sequence version replaced XM_678548.2. ##Evidence-Data-START## Transcript exon combination :: BC093246.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTTGTCCT ATCTTGCTCG CTGGGTGTCC AGAGGTGCTG TCCAGGCTCT AAAAGGAACA 
AGCAGTCGGC CTCAACTGGT CAAGACACCT CGTATCGGCA ATCAGACTTT GGCAGCGGCT 
CCTCTGCCCT TTGCTGGGGG CAGTCCAGGA GTGAGACAGG CCCGGGCACT GGATCAGGAG 
AGATTACTGC AGATCGACTG GGATGATGGG AGCTGTAGTT TGTATCCATT CACCTGGTTG 
AGGGATAACT GCCGGTGTCC GCTCTGTACG CTGCAGTCGG CTCAAGCACG CAGCCTGCTG 
TTCTCACATC TGGACGTGCA CACCGGGATG GATCACGTAC AACTGATGGA TAATAAGGTG 
TCCATAACTT GGCCTGATCA GCACAGCAGT GAGTTTGATC CTGACTGGCT GAAGAAACGC 
TGCTTTTCCT CTGAGGCCAG ACAGGCTCTG CAGGAGGAGC TCTTCCTTAA TGAGCGTGAG 
TATTGGGACT CTGGTCTCCA GATCCCCACA GCAGATTTCG AGGAGGTTCT ACATGACGAC 
AAGGCTGCAC TGGCCTGGTT AGAAGCTCTG AGACGCATTG GCATAGTCTA CCTGAGGGGG 
GCACCAGCGG AACAGGGCCA GTTGGCCAGA CTCAGCCAGA GAATTGGCTA CCTGCGCCTC 
ACCTTTTATG GACACACGTG GCAAGTGCAG GACAAGCCCA TGGCTAACAA TGTTGCCTAC 
ACTTCTGGAG AACTGAGTCT GCACACAGAT TACCCTGCTC TCCATCATCC ACCTGGGGTG 
CAGTTCCTAC ATTGTGTCCG TCAGGCTGAT GAGGGTGGTG AGAGTGAGGT GGTTGATGGG 
TTTCACATGG CAGAGCAGCT CCGCAGGGAA GATCCAGAGG CGTTTAACAT CCTCTCCTCT 
CTCAGGGTGG ACTTCACAGA CAGCGGTGCT GACTACTGCG ACTTCAGTGT GCAGTCCAAA 
AACCACATTA TAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017717.1
CDS	167..1144
Translation	MLSYLARWVSRGAVQALKGTSSRPQLVKTPRIGNQTLAAAPLPFAGGSPGVRQARALDQERLLQIDWDDGSCSLYPFTWLRDNCRCPLCTLQSAQARSLLFSHLDVHTGMDHVQLMDNKVSITWPDQHSSEFDPDWLKKRCFSSEARQALQEELFLNEREYWDSGLQIPTADFEEVLHDDKAALAWLEALRRIGIVYLRGAPAEQGQLARLSQRIGYLRLTFYGHTWQVQDKPMANNVAYTSGELSLHTDYPALHHPPGVQFLHCVRQADEGGESEVVDGFHMAEQLRREDPEAFNILSSLRVDFTDSGADYCDFSVQSKNHIIE

Target ORF information:

RefSeq Version	NM_001017717.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017717.1

ORF Insert Sequence:

ATGTTGTCCT ATCTTGCTCG CTGGGTGTCC AGAGGTGCTG TCCAGGCTCT AAAAGGAACA 
AGCAGTCGGC CTCAACTGGT CAAGACACCT CGTATCGGCA ATCAGACTTT GGCAGCGGCT 
CCTCTGCCCT TTGCTGGGGG CAGTCCAGGA GTGAGACAGG CCCGGGCACT GGATCAGGAG 
AGATTACTGC AGATCGACTG GGATGATGGG AGCTGTAGTT TGTATCCATT CACCTGGTTG 
AGGGATAACT GCCGGTGTCC GCTCTGTACG CTGCAGTCGG CTCAAGCACG CAGCCTGCTG 
TTCTCACATC TGGACGTGCA CACCGGGATG GATCACGTAC AACTGATGGA TAATAAGGTG 
TCCATAACTT GGCCTGATCA GCACAGCAGT GAGTTTGATC CTGACTGGCT GAAGAAACGC 
TGCTTTTCCT CTGAGGCCAG ACAGGCTCTG CAGGAGGAGC TCTTCCTTAA TGAGCGTGAG 
TATTGGGACT CTGGTCTCCA GATCCCCACA GCAGATTTCG AGGAGGTTCT ACATGACGAC 
AAGGCTGCAC TGGCCTGGTT AGAAGCTCTG AGACGCATTG GCATAGTCTA CCTGAGGGGG 
GCACCAGCGG AACAGGGCCA GTTGGCCAGA CTCAGCCAGA GAATTGGCTA CCTGCGCCTC 
ACCTTTTATG GACACACGTG GCAAGTGCAG GACAAGCCCA TGGCTAACAA TGTTGCCTAC 
ACTTCTGGAG AACTGAGTCT GCACACAGAT TACCCTGCTC TCCATCATCC ACCTGGGGTG 
CAGTTCCTAC ATTGTGTCCG TCAGGCTGAT GAGGGTGGTG AGAGTGAGGT GGTTGATGGG 
TTTCACATGG CAGAGCAGCT CCGCAGGGAA GATCCAGAGG CGTTTAACAT CCTCTCCTCT 
CTCAGGGTGG ACTTCACAGA CAGCGGTGCT GACTACTGCG ACTTCAGTGT GCAGTCCAAA 
AACCACATTA TAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbox1 ( XM_005168903.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168960.1 Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa13092
Clone ID Related Accession (Same CDS sequence)	XM_005168903.4 , XM_005168904.3 , XM_005168903.3 , XM_005168904.4 , XM_005168905.3 , XM_021477587.1
Accession Version	XM_005168903.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	gamma-butyrobetaine dioxygenase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168903.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCCT ATCTTGCTCG CTGGGTGTCC AGAGGTGCTG TCCAGGCTCT AAAAGGAACA 
AGCAGTCGGC CTCAACTGGT CAAGACACCT CGTATCGGCA ATCAGACTTT GGCAGCGGCT 
CCTCTGCCCT TTGCTGGGGG CAGTCCAGGA GTGAGACAGG CCCGGGCACT GGATCAGGAG 
AGATTACTGC AGATCGACTG GGATGATGGG AGCTGTAGTT TGTATCCATT CACCTGGTTG 
AGGGATAACT GCCAGTGTCC GCTCTGTACG CTGCAGTCGG CTCAAGCACG CAGCCTGCTG 
TTCTCACATC TGGACGTGCA CACCGGGATG GATCACGTAC AACTGATGGA TAATAAGGTG 
TCCATAACTT GGCCTGATCA GCACAGCAGT GAGTTTGATC CTGACTGGCT GAAGAAACGC 
TGCTTTTCCT CTGAGGCCAG ACAGGCTCTG CAGGAGGAGC TCTTCCTTAA TGAGCGTGAG 
TATTGGGACT CTGGTCTCCA GATCCCCACA GCAGATTTCG AGGAGGTTCT ACATGACGAC 
AAGGCTGCAC TGGCCTGGTT AGAAGCTCTG AGACGCATTG GCATAGTCTA CCTGAGGGGG 
GCACCAGCGG AACAGGGCCA GTTGGCCAGA CTCAGCCAGA GAATTGGCTA CCTGCGCCTC 
ACCTTTTATG GACACACGTG GCAAGTGCAG GACAAGCCCA TGGCTAACAA TGTTGCCTAC 
ACTTCTGGAG AACTGAGTCT GCACACAGAT TACCCTGCTC TCCATCATCC ACCTGGGGTG 
CAGTTCCTAC ATTGTGTCCG TCAGGCTGAT GAGGGTGGTG AGAGTGAGGT GGTTGATGGG 
TTTCACATGG CAGAGCAGCT CCGCAGGGAA GATCCAGAGG CGTTTAACAT CCTCTCCTCT 
CTCAGGGTGG ACTTCACAGA CAGCGGTGCT GACTACTGCG ACTTCAGTGT GCAGTCCAAA 
AACCACATTA TAGATGTGGA CTCATCCGGT CGGGTTGTGA GGATCAACTA TAATAATGCC 
ACTCGAGACT CAGTGCTGGA TCTGCCTCTG CATCAGGTTC AGCCCTTCTA CTCGTCTCTG 
AAGGCCTTCG TGGAGCTGCT CAGCCGGCCG GAGAATGTCT TCACTTACAG AATGGAGCCA 
GGTGATGTGG TGACCTTCGA TAACTGGCGT CTGCTGCACG GCCGAAAGAG CTACCAGTCT 
CGAGGGCAGA ACTTAAGACA TCTAGAAGGA GCGTATCTGG ACTGGGACGA GGTCATGTCC 
CGTCTCAGGA TCCTCCGGAA GACGGTCCGC GGAGACAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168960.1
CDS	172..1473
Translation	MLSYLARWVSRGAVQALKGTSSRPQLVKTPRIGNQTLAAAPLPFAGGSPGVRQARALDQERLLQIDWDDGSCSLYPFTWLRDNCQCPLCTLQSAQARSLLFSHLDVHTGMDHVQLMDNKVSITWPDQHSSEFDPDWLKKRCFSSEARQALQEELFLNEREYWDSGLQIPTADFEEVLHDDKAALAWLEALRRIGIVYLRGAPAEQGQLARLSQRIGYLRLTFYGHTWQVQDKPMANNVAYTSGELSLHTDYPALHHPPGVQFLHCVRQADEGGESEVVDGFHMAEQLRREDPEAFNILSSLRVDFTDSGADYCDFSVQSKNHIIDVDSSGRVVRINYNNATRDSVLDLPLHQVQPFYSSLKAFVELLSRPENVFTYRMEPGDVVTFDNWRLLHGRKSYQSRGQNLRHLEGAYLDWDEVMSRLRILRKTVRGDS

Target ORF information:

RefSeq Version	XM_005168903.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168903.4

ORF Insert Sequence:

ATGTTGTCCT ATCTTGCTCG CTGGGTGTCC AGAGGTGCTG TCCAGGCTCT AAAAGGAACA 
AGCAGTCGGC CTCAACTGGT CAAGACACCT CGTATCGGCA ATCAGACTTT GGCAGCGGCT 
CCTCTGCCCT TTGCTGGGGG CAGTCCAGGA GTGAGACAGG CCCGGGCACT GGATCAGGAG 
AGATTACTGC AGATCGACTG GGATGATGGG AGCTGTAGTT TGTATCCATT CACCTGGTTG 
AGGGATAACT GCCAGTGTCC GCTCTGTACG CTGCAGTCGG CTCAAGCACG CAGCCTGCTG 
TTCTCACATC TGGACGTGCA CACCGGGATG GATCACGTAC AACTGATGGA TAATAAGGTG 
TCCATAACTT GGCCTGATCA GCACAGCAGT GAGTTTGATC CTGACTGGCT GAAGAAACGC 
TGCTTTTCCT CTGAGGCCAG ACAGGCTCTG CAGGAGGAGC TCTTCCTTAA TGAGCGTGAG 
TATTGGGACT CTGGTCTCCA GATCCCCACA GCAGATTTCG AGGAGGTTCT ACATGACGAC 
AAGGCTGCAC TGGCCTGGTT AGAAGCTCTG AGACGCATTG GCATAGTCTA CCTGAGGGGG 
GCACCAGCGG AACAGGGCCA GTTGGCCAGA CTCAGCCAGA GAATTGGCTA CCTGCGCCTC 
ACCTTTTATG GACACACGTG GCAAGTGCAG GACAAGCCCA TGGCTAACAA TGTTGCCTAC 
ACTTCTGGAG AACTGAGTCT GCACACAGAT TACCCTGCTC TCCATCATCC ACCTGGGGTG 
CAGTTCCTAC ATTGTGTCCG TCAGGCTGAT GAGGGTGGTG AGAGTGAGGT GGTTGATGGG 
TTTCACATGG CAGAGCAGCT CCGCAGGGAA GATCCAGAGG CGTTTAACAT CCTCTCCTCT 
CTCAGGGTGG ACTTCACAGA CAGCGGTGCT GACTACTGCG ACTTCAGTGT GCAGTCCAAA 
AACCACATTA TAGATGTGGA CTCATCCGGT CGGGTTGTGA GGATCAACTA TAATAATGCC 
ACTCGAGACT CAGTGCTGGA TCTGCCTCTG CATCAGGTTC AGCCCTTCTA CTCGTCTCTG 
AAGGCCTTCG TGGAGCTGCT CAGCCGGCCG GAGAATGTCT TCACTTACAG AATGGAGCCA 
GGTGATGTGG TGACCTTCGA TAACTGGCGT CTGCTGCACG GCCGAAAGAG CTACCAGTCT 
CGAGGGCAGA ACTTAAGACA TCTAGAAGGA GCGTATCTGG ACTGGGACGA GGTCATGTCC 
CGTCTCAGGA TCCTCCGGAA GACGGTCCGC GGAGACAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbox1 ( XM_005168904.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168961.1 Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa13092
Clone ID Related Accession (Same CDS sequence)	XM_005168903.4 , XM_005168904.3 , XM_005168903.3 , XM_005168904.4 , XM_005168905.3 , XM_021477587.1
Accession Version	XM_005168904.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	gamma-butyrobetaine dioxygenase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168904.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCCT ATCTTGCTCG CTGGGTGTCC AGAGGTGCTG TCCAGGCTCT AAAAGGAACA 
AGCAGTCGGC CTCAACTGGT CAAGACACCT CGTATCGGCA ATCAGACTTT GGCAGCGGCT 
CCTCTGCCCT TTGCTGGGGG CAGTCCAGGA GTGAGACAGG CCCGGGCACT GGATCAGGAG 
AGATTACTGC AGATCGACTG GGATGATGGG AGCTGTAGTT TGTATCCATT CACCTGGTTG 
AGGGATAACT GCCAGTGTCC GCTCTGTACG CTGCAGTCGG CTCAAGCACG CAGCCTGCTG 
TTCTCACATC TGGACGTGCA CACCGGGATG GATCACGTAC AACTGATGGA TAATAAGGTG 
TCCATAACTT GGCCTGATCA GCACAGCAGT GAGTTTGATC CTGACTGGCT GAAGAAACGC 
TGCTTTTCCT CTGAGGCCAG ACAGGCTCTG CAGGAGGAGC TCTTCCTTAA TGAGCGTGAG 
TATTGGGACT CTGGTCTCCA GATCCCCACA GCAGATTTCG AGGAGGTTCT ACATGACGAC 
AAGGCTGCAC TGGCCTGGTT AGAAGCTCTG AGACGCATTG GCATAGTCTA CCTGAGGGGG 
GCACCAGCGG AACAGGGCCA GTTGGCCAGA CTCAGCCAGA GAATTGGCTA CCTGCGCCTC 
ACCTTTTATG GACACACGTG GCAAGTGCAG GACAAGCCCA TGGCTAACAA TGTTGCCTAC 
ACTTCTGGAG AACTGAGTCT GCACACAGAT TACCCTGCTC TCCATCATCC ACCTGGGGTG 
CAGTTCCTAC ATTGTGTCCG TCAGGCTGAT GAGGGTGGTG AGAGTGAGGT GGTTGATGGG 
TTTCACATGG CAGAGCAGCT CCGCAGGGAA GATCCAGAGG CGTTTAACAT CCTCTCCTCT 
CTCAGGGTGG ACTTCACAGA CAGCGGTGCT GACTACTGCG ACTTCAGTGT GCAGTCCAAA 
AACCACATTA TAGATGTGGA CTCATCCGGT CGGGTTGTGA GGATCAACTA TAATAATGCC 
ACTCGAGACT CAGTGCTGGA TCTGCCTCTG CATCAGGTTC AGCCCTTCTA CTCGTCTCTG 
AAGGCCTTCG TGGAGCTGCT CAGCCGGCCG GAGAATGTCT TCACTTACAG AATGGAGCCA 
GGTGATGTGG TGACCTTCGA TAACTGGCGT CTGCTGCACG GCCGAAAGAG CTACCAGTCT 
CGAGGGCAGA ACTTAAGACA TCTAGAAGGA GCGTATCTGG ACTGGGACGA GGTCATGTCC 
CGTCTCAGGA TCCTCCGGAA GACGGTCCGC GGAGACAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168961.1
CDS	155..1456
Translation	MLSYLARWVSRGAVQALKGTSSRPQLVKTPRIGNQTLAAAPLPFAGGSPGVRQARALDQERLLQIDWDDGSCSLYPFTWLRDNCQCPLCTLQSAQARSLLFSHLDVHTGMDHVQLMDNKVSITWPDQHSSEFDPDWLKKRCFSSEARQALQEELFLNEREYWDSGLQIPTADFEEVLHDDKAALAWLEALRRIGIVYLRGAPAEQGQLARLSQRIGYLRLTFYGHTWQVQDKPMANNVAYTSGELSLHTDYPALHHPPGVQFLHCVRQADEGGESEVVDGFHMAEQLRREDPEAFNILSSLRVDFTDSGADYCDFSVQSKNHIIDVDSSGRVVRINYNNATRDSVLDLPLHQVQPFYSSLKAFVELLSRPENVFTYRMEPGDVVTFDNWRLLHGRKSYQSRGQNLRHLEGAYLDWDEVMSRLRILRKTVRGDS

Target ORF information:

RefSeq Version	XM_005168904.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168904.3

ORF Insert Sequence:

ATGTTGTCCT ATCTTGCTCG CTGGGTGTCC AGAGGTGCTG TCCAGGCTCT AAAAGGAACA 
AGCAGTCGGC CTCAACTGGT CAAGACACCT CGTATCGGCA ATCAGACTTT GGCAGCGGCT 
CCTCTGCCCT TTGCTGGGGG CAGTCCAGGA GTGAGACAGG CCCGGGCACT GGATCAGGAG 
AGATTACTGC AGATCGACTG GGATGATGGG AGCTGTAGTT TGTATCCATT CACCTGGTTG 
AGGGATAACT GCCAGTGTCC GCTCTGTACG CTGCAGTCGG CTCAAGCACG CAGCCTGCTG 
TTCTCACATC TGGACGTGCA CACCGGGATG GATCACGTAC AACTGATGGA TAATAAGGTG 
TCCATAACTT GGCCTGATCA GCACAGCAGT GAGTTTGATC CTGACTGGCT GAAGAAACGC 
TGCTTTTCCT CTGAGGCCAG ACAGGCTCTG CAGGAGGAGC TCTTCCTTAA TGAGCGTGAG 
TATTGGGACT CTGGTCTCCA GATCCCCACA GCAGATTTCG AGGAGGTTCT ACATGACGAC 
AAGGCTGCAC TGGCCTGGTT AGAAGCTCTG AGACGCATTG GCATAGTCTA CCTGAGGGGG 
GCACCAGCGG AACAGGGCCA GTTGGCCAGA CTCAGCCAGA GAATTGGCTA CCTGCGCCTC 
ACCTTTTATG GACACACGTG GCAAGTGCAG GACAAGCCCA TGGCTAACAA TGTTGCCTAC 
ACTTCTGGAG AACTGAGTCT GCACACAGAT TACCCTGCTC TCCATCATCC ACCTGGGGTG 
CAGTTCCTAC ATTGTGTCCG TCAGGCTGAT GAGGGTGGTG AGAGTGAGGT GGTTGATGGG 
TTTCACATGG CAGAGCAGCT CCGCAGGGAA GATCCAGAGG CGTTTAACAT CCTCTCCTCT 
CTCAGGGTGG ACTTCACAGA CAGCGGTGCT GACTACTGCG ACTTCAGTGT GCAGTCCAAA 
AACCACATTA TAGATGTGGA CTCATCCGGT CGGGTTGTGA GGATCAACTA TAATAATGCC 
ACTCGAGACT CAGTGCTGGA TCTGCCTCTG CATCAGGTTC AGCCCTTCTA CTCGTCTCTG 
AAGGCCTTCG TGGAGCTGCT CAGCCGGCCG GAGAATGTCT TCACTTACAG AATGGAGCCA 
GGTGATGTGG TGACCTTCGA TAACTGGCGT CTGCTGCACG GCCGAAAGAG CTACCAGTCT 
CGAGGGCAGA ACTTAAGACA TCTAGAAGGA GCGTATCTGG ACTGGGACGA GGTCATGTCC 
CGTCTCAGGA TCCTCCGGAA GACGGTCCGC GGAGACAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbox1 ( XM_005168903.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168960.1 Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa13092
Clone ID Related Accession (Same CDS sequence)	XM_005168903.4 , XM_005168904.3 , XM_005168903.3 , XM_005168904.4 , XM_005168905.3 , XM_021477587.1
Accession Version	XM_005168903.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	gamma-butyrobetaine dioxygenase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168903.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCCT ATCTTGCTCG CTGGGTGTCC AGAGGTGCTG TCCAGGCTCT AAAAGGAACA 
AGCAGTCGGC CTCAACTGGT CAAGACACCT CGTATCGGCA ATCAGACTTT GGCAGCGGCT 
CCTCTGCCCT TTGCTGGGGG CAGTCCAGGA GTGAGACAGG CCCGGGCACT GGATCAGGAG 
AGATTACTGC AGATCGACTG GGATGATGGG AGCTGTAGTT TGTATCCATT CACCTGGTTG 
AGGGATAACT GCCAGTGTCC GCTCTGTACG CTGCAGTCGG CTCAAGCACG CAGCCTGCTG 
TTCTCACATC TGGACGTGCA CACCGGGATG GATCACGTAC AACTGATGGA TAATAAGGTG 
TCCATAACTT GGCCTGATCA GCACAGCAGT GAGTTTGATC CTGACTGGCT GAAGAAACGC 
TGCTTTTCCT CTGAGGCCAG ACAGGCTCTG CAGGAGGAGC TCTTCCTTAA TGAGCGTGAG 
TATTGGGACT CTGGTCTCCA GATCCCCACA GCAGATTTCG AGGAGGTTCT ACATGACGAC 
AAGGCTGCAC TGGCCTGGTT AGAAGCTCTG AGACGCATTG GCATAGTCTA CCTGAGGGGG 
GCACCAGCGG AACAGGGCCA GTTGGCCAGA CTCAGCCAGA GAATTGGCTA CCTGCGCCTC 
ACCTTTTATG GACACACGTG GCAAGTGCAG GACAAGCCCA TGGCTAACAA TGTTGCCTAC 
ACTTCTGGAG AACTGAGTCT GCACACAGAT TACCCTGCTC TCCATCATCC ACCTGGGGTG 
CAGTTCCTAC ATTGTGTCCG TCAGGCTGAT GAGGGTGGTG AGAGTGAGGT GGTTGATGGG 
TTTCACATGG CAGAGCAGCT CCGCAGGGAA GATCCAGAGG CGTTTAACAT CCTCTCCTCT 
CTCAGGGTGG ACTTCACAGA CAGCGGTGCT GACTACTGCG ACTTCAGTGT GCAGTCCAAA 
AACCACATTA TAGATGTGGA CTCATCCGGT CGGGTTGTGA GGATCAACTA TAATAATGCC 
ACTCGAGACT CAGTGCTGGA TCTGCCTCTG CATCAGGTTC AGCCCTTCTA CTCGTCTCTG 
AAGGCCTTCG TGGAGCTGCT CAGCCGGCCG GAGAATGTCT TCACTTACAG AATGGAGCCA 
GGTGATGTGG TGACCTTCGA TAACTGGCGT CTGCTGCACG GCCGAAAGAG CTACCAGTCT 
CGAGGGCAGA ACTTAAGACA TCTAGAAGGA GCGTATCTGG ACTGGGACGA GGTCATGTCC 
CGTCTCAGGA TCCTCCGGAA GACGGTCCGC GGAGACAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168960.1
CDS	152..1453
Translation	MLSYLARWVSRGAVQALKGTSSRPQLVKTPRIGNQTLAAAPLPFAGGSPGVRQARALDQERLLQIDWDDGSCSLYPFTWLRDNCQCPLCTLQSAQARSLLFSHLDVHTGMDHVQLMDNKVSITWPDQHSSEFDPDWLKKRCFSSEARQALQEELFLNEREYWDSGLQIPTADFEEVLHDDKAALAWLEALRRIGIVYLRGAPAEQGQLARLSQRIGYLRLTFYGHTWQVQDKPMANNVAYTSGELSLHTDYPALHHPPGVQFLHCVRQADEGGESEVVDGFHMAEQLRREDPEAFNILSSLRVDFTDSGADYCDFSVQSKNHIIDVDSSGRVVRINYNNATRDSVLDLPLHQVQPFYSSLKAFVELLSRPENVFTYRMEPGDVVTFDNWRLLHGRKSYQSRGQNLRHLEGAYLDWDEVMSRLRILRKTVRGDS

Target ORF information:

RefSeq Version	XM_005168903.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168903.3

ORF Insert Sequence:

ATGTTGTCCT ATCTTGCTCG CTGGGTGTCC AGAGGTGCTG TCCAGGCTCT AAAAGGAACA 
AGCAGTCGGC CTCAACTGGT CAAGACACCT CGTATCGGCA ATCAGACTTT GGCAGCGGCT 
CCTCTGCCCT TTGCTGGGGG CAGTCCAGGA GTGAGACAGG CCCGGGCACT GGATCAGGAG 
AGATTACTGC AGATCGACTG GGATGATGGG AGCTGTAGTT TGTATCCATT CACCTGGTTG 
AGGGATAACT GCCAGTGTCC GCTCTGTACG CTGCAGTCGG CTCAAGCACG CAGCCTGCTG 
TTCTCACATC TGGACGTGCA CACCGGGATG GATCACGTAC AACTGATGGA TAATAAGGTG 
TCCATAACTT GGCCTGATCA GCACAGCAGT GAGTTTGATC CTGACTGGCT GAAGAAACGC 
TGCTTTTCCT CTGAGGCCAG ACAGGCTCTG CAGGAGGAGC TCTTCCTTAA TGAGCGTGAG 
TATTGGGACT CTGGTCTCCA GATCCCCACA GCAGATTTCG AGGAGGTTCT ACATGACGAC 
AAGGCTGCAC TGGCCTGGTT AGAAGCTCTG AGACGCATTG GCATAGTCTA CCTGAGGGGG 
GCACCAGCGG AACAGGGCCA GTTGGCCAGA CTCAGCCAGA GAATTGGCTA CCTGCGCCTC 
ACCTTTTATG GACACACGTG GCAAGTGCAG GACAAGCCCA TGGCTAACAA TGTTGCCTAC 
ACTTCTGGAG AACTGAGTCT GCACACAGAT TACCCTGCTC TCCATCATCC ACCTGGGGTG 
CAGTTCCTAC ATTGTGTCCG TCAGGCTGAT GAGGGTGGTG AGAGTGAGGT GGTTGATGGG 
TTTCACATGG CAGAGCAGCT CCGCAGGGAA GATCCAGAGG CGTTTAACAT CCTCTCCTCT 
CTCAGGGTGG ACTTCACAGA CAGCGGTGCT GACTACTGCG ACTTCAGTGT GCAGTCCAAA 
AACCACATTA TAGATGTGGA CTCATCCGGT CGGGTTGTGA GGATCAACTA TAATAATGCC 
ACTCGAGACT CAGTGCTGGA TCTGCCTCTG CATCAGGTTC AGCCCTTCTA CTCGTCTCTG 
AAGGCCTTCG TGGAGCTGCT CAGCCGGCCG GAGAATGTCT TCACTTACAG AATGGAGCCA 
GGTGATGTGG TGACCTTCGA TAACTGGCGT CTGCTGCACG GCCGAAAGAG CTACCAGTCT 
CGAGGGCAGA ACTTAAGACA TCTAGAAGGA GCGTATCTGG ACTGGGACGA GGTCATGTCC 
CGTCTCAGGA TCCTCCGGAA GACGGTCCGC GGAGACAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbox1 ( XM_005168904.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168961.1 Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa13092
Clone ID Related Accession (Same CDS sequence)	XM_005168903.4 , XM_005168904.3 , XM_005168903.3 , XM_005168904.4 , XM_005168905.3 , XM_021477587.1
Accession Version	XM_005168904.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	gamma-butyrobetaine dioxygenase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168904.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCCT ATCTTGCTCG CTGGGTGTCC AGAGGTGCTG TCCAGGCTCT AAAAGGAACA 
AGCAGTCGGC CTCAACTGGT CAAGACACCT CGTATCGGCA ATCAGACTTT GGCAGCGGCT 
CCTCTGCCCT TTGCTGGGGG CAGTCCAGGA GTGAGACAGG CCCGGGCACT GGATCAGGAG 
AGATTACTGC AGATCGACTG GGATGATGGG AGCTGTAGTT TGTATCCATT CACCTGGTTG 
AGGGATAACT GCCAGTGTCC GCTCTGTACG CTGCAGTCGG CTCAAGCACG CAGCCTGCTG 
TTCTCACATC TGGACGTGCA CACCGGGATG GATCACGTAC AACTGATGGA TAATAAGGTG 
TCCATAACTT GGCCTGATCA GCACAGCAGT GAGTTTGATC CTGACTGGCT GAAGAAACGC 
TGCTTTTCCT CTGAGGCCAG ACAGGCTCTG CAGGAGGAGC TCTTCCTTAA TGAGCGTGAG 
TATTGGGACT CTGGTCTCCA GATCCCCACA GCAGATTTCG AGGAGGTTCT ACATGACGAC 
AAGGCTGCAC TGGCCTGGTT AGAAGCTCTG AGACGCATTG GCATAGTCTA CCTGAGGGGG 
GCACCAGCGG AACAGGGCCA GTTGGCCAGA CTCAGCCAGA GAATTGGCTA CCTGCGCCTC 
ACCTTTTATG GACACACGTG GCAAGTGCAG GACAAGCCCA TGGCTAACAA TGTTGCCTAC 
ACTTCTGGAG AACTGAGTCT GCACACAGAT TACCCTGCTC TCCATCATCC ACCTGGGGTG 
CAGTTCCTAC ATTGTGTCCG TCAGGCTGAT GAGGGTGGTG AGAGTGAGGT GGTTGATGGG 
TTTCACATGG CAGAGCAGCT CCGCAGGGAA GATCCAGAGG CGTTTAACAT CCTCTCCTCT 
CTCAGGGTGG ACTTCACAGA CAGCGGTGCT GACTACTGCG ACTTCAGTGT GCAGTCCAAA 
AACCACATTA TAGATGTGGA CTCATCCGGT CGGGTTGTGA GGATCAACTA TAATAATGCC 
ACTCGAGACT CAGTGCTGGA TCTGCCTCTG CATCAGGTTC AGCCCTTCTA CTCGTCTCTG 
AAGGCCTTCG TGGAGCTGCT CAGCCGGCCG GAGAATGTCT TCACTTACAG AATGGAGCCA 
GGTGATGTGG TGACCTTCGA TAACTGGCGT CTGCTGCACG GCCGAAAGAG CTACCAGTCT 
CGAGGGCAGA ACTTAAGACA TCTAGAAGGA GCGTATCTGG ACTGGGACGA GGTCATGTCC 
CGTCTCAGGA TCCTCCGGAA GACGGTCCGC GGAGACAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168961.1
CDS	166..1467
Translation	MLSYLARWVSRGAVQALKGTSSRPQLVKTPRIGNQTLAAAPLPFAGGSPGVRQARALDQERLLQIDWDDGSCSLYPFTWLRDNCQCPLCTLQSAQARSLLFSHLDVHTGMDHVQLMDNKVSITWPDQHSSEFDPDWLKKRCFSSEARQALQEELFLNEREYWDSGLQIPTADFEEVLHDDKAALAWLEALRRIGIVYLRGAPAEQGQLARLSQRIGYLRLTFYGHTWQVQDKPMANNVAYTSGELSLHTDYPALHHPPGVQFLHCVRQADEGGESEVVDGFHMAEQLRREDPEAFNILSSLRVDFTDSGADYCDFSVQSKNHIIDVDSSGRVVRINYNNATRDSVLDLPLHQVQPFYSSLKAFVELLSRPENVFTYRMEPGDVVTFDNWRLLHGRKSYQSRGQNLRHLEGAYLDWDEVMSRLRILRKTVRGDS

Target ORF information:

RefSeq Version	XM_005168904.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168904.4

ORF Insert Sequence:

ATGTTGTCCT ATCTTGCTCG CTGGGTGTCC AGAGGTGCTG TCCAGGCTCT AAAAGGAACA 
AGCAGTCGGC CTCAACTGGT CAAGACACCT CGTATCGGCA ATCAGACTTT GGCAGCGGCT 
CCTCTGCCCT TTGCTGGGGG CAGTCCAGGA GTGAGACAGG CCCGGGCACT GGATCAGGAG 
AGATTACTGC AGATCGACTG GGATGATGGG AGCTGTAGTT TGTATCCATT CACCTGGTTG 
AGGGATAACT GCCAGTGTCC GCTCTGTACG CTGCAGTCGG CTCAAGCACG CAGCCTGCTG 
TTCTCACATC TGGACGTGCA CACCGGGATG GATCACGTAC AACTGATGGA TAATAAGGTG 
TCCATAACTT GGCCTGATCA GCACAGCAGT GAGTTTGATC CTGACTGGCT GAAGAAACGC 
TGCTTTTCCT CTGAGGCCAG ACAGGCTCTG CAGGAGGAGC TCTTCCTTAA TGAGCGTGAG 
TATTGGGACT CTGGTCTCCA GATCCCCACA GCAGATTTCG AGGAGGTTCT ACATGACGAC 
AAGGCTGCAC TGGCCTGGTT AGAAGCTCTG AGACGCATTG GCATAGTCTA CCTGAGGGGG 
GCACCAGCGG AACAGGGCCA GTTGGCCAGA CTCAGCCAGA GAATTGGCTA CCTGCGCCTC 
ACCTTTTATG GACACACGTG GCAAGTGCAG GACAAGCCCA TGGCTAACAA TGTTGCCTAC 
ACTTCTGGAG AACTGAGTCT GCACACAGAT TACCCTGCTC TCCATCATCC ACCTGGGGTG 
CAGTTCCTAC ATTGTGTCCG TCAGGCTGAT GAGGGTGGTG AGAGTGAGGT GGTTGATGGG 
TTTCACATGG CAGAGCAGCT CCGCAGGGAA GATCCAGAGG CGTTTAACAT CCTCTCCTCT 
CTCAGGGTGG ACTTCACAGA CAGCGGTGCT GACTACTGCG ACTTCAGTGT GCAGTCCAAA 
AACCACATTA TAGATGTGGA CTCATCCGGT CGGGTTGTGA GGATCAACTA TAATAATGCC 
ACTCGAGACT CAGTGCTGGA TCTGCCTCTG CATCAGGTTC AGCCCTTCTA CTCGTCTCTG 
AAGGCCTTCG TGGAGCTGCT CAGCCGGCCG GAGAATGTCT TCACTTACAG AATGGAGCCA 
GGTGATGTGG TGACCTTCGA TAACTGGCGT CTGCTGCACG GCCGAAAGAG CTACCAGTCT 
CGAGGGCAGA ACTTAAGACA TCTAGAAGGA GCGTATCTGG ACTGGGACGA GGTCATGTCC 
CGTCTCAGGA TCCTCCGGAA GACGGTCCGC GGAGACAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbox1 ( XM_005168905.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168962.1 Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa13092
Clone ID Related Accession (Same CDS sequence)	XM_005168903.4 , XM_005168904.3 , XM_005168903.3 , XM_005168904.4 , XM_005168905.3 , XM_021477587.1
Accession Version	XM_005168905.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	gamma-butyrobetaine dioxygenase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168905.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCCT ATCTTGCTCG CTGGGTGTCC AGAGGTGCTG TCCAGGCTCT AAAAGGAACA 
AGCAGTCGGC CTCAACTGGT CAAGACACCT CGTATCGGCA ATCAGACTTT GGCAGCGGCT 
CCTCTGCCCT TTGCTGGGGG CAGTCCAGGA GTGAGACAGG CCCGGGCACT GGATCAGGAG 
AGATTACTGC AGATCGACTG GGATGATGGG AGCTGTAGTT TGTATCCATT CACCTGGTTG 
AGGGATAACT GCCAGTGTCC GCTCTGTACG CTGCAGTCGG CTCAAGCACG CAGCCTGCTG 
TTCTCACATC TGGACGTGCA CACCGGGATG GATCACGTAC AACTGATGGA TAATAAGGTG 
TCCATAACTT GGCCTGATCA GCACAGCAGT GAGTTTGATC CTGACTGGCT GAAGAAACGC 
TGCTTTTCCT CTGAGGCCAG ACAGGCTCTG CAGGAGGAGC TCTTCCTTAA TGAGCGTGAG 
TATTGGGACT CTGGTCTCCA GATCCCCACA GCAGATTTCG AGGAGGTTCT ACATGACGAC 
AAGGCTGCAC TGGCCTGGTT AGAAGCTCTG AGACGCATTG GCATAGTCTA CCTGAGGGGG 
GCACCAGCGG AACAGGGCCA GTTGGCCAGA CTCAGCCAGA GAATTGGCTA CCTGCGCCTC 
ACCTTTTATG GACACACGTG GCAAGTGCAG GACAAGCCCA TGGCTAACAA TGTTGCCTAC 
ACTTCTGGAG AACTGAGTCT GCACACAGAT TACCCTGCTC TCCATCATCC ACCTGGGGTG 
CAGTTCCTAC ATTGTGTCCG TCAGGCTGAT GAGGGTGGTG AGAGTGAGGT GGTTGATGGG 
TTTCACATGG CAGAGCAGCT CCGCAGGGAA GATCCAGAGG CGTTTAACAT CCTCTCCTCT 
CTCAGGGTGG ACTTCACAGA CAGCGGTGCT GACTACTGCG ACTTCAGTGT GCAGTCCAAA 
AACCACATTA TAGATGTGGA CTCATCCGGT CGGGTTGTGA GGATCAACTA TAATAATGCC 
ACTCGAGACT CAGTGCTGGA TCTGCCTCTG CATCAGGTTC AGCCCTTCTA CTCGTCTCTG 
AAGGCCTTCG TGGAGCTGCT CAGCCGGCCG GAGAATGTCT TCACTTACAG AATGGAGCCA 
GGTGATGTGG TGACCTTCGA TAACTGGCGT CTGCTGCACG GCCGAAAGAG CTACCAGTCT 
CGAGGGCAGA ACTTAAGACA TCTAGAAGGA GCGTATCTGG ACTGGGACGA GGTCATGTCC 
CGTCTCAGGA TCCTCCGGAA GACGGTCCGC GGAGACAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168962.1
CDS	162..1463
Translation	MLSYLARWVSRGAVQALKGTSSRPQLVKTPRIGNQTLAAAPLPFAGGSPGVRQARALDQERLLQIDWDDGSCSLYPFTWLRDNCQCPLCTLQSAQARSLLFSHLDVHTGMDHVQLMDNKVSITWPDQHSSEFDPDWLKKRCFSSEARQALQEELFLNEREYWDSGLQIPTADFEEVLHDDKAALAWLEALRRIGIVYLRGAPAEQGQLARLSQRIGYLRLTFYGHTWQVQDKPMANNVAYTSGELSLHTDYPALHHPPGVQFLHCVRQADEGGESEVVDGFHMAEQLRREDPEAFNILSSLRVDFTDSGADYCDFSVQSKNHIIDVDSSGRVVRINYNNATRDSVLDLPLHQVQPFYSSLKAFVELLSRPENVFTYRMEPGDVVTFDNWRLLHGRKSYQSRGQNLRHLEGAYLDWDEVMSRLRILRKTVRGDS

Target ORF information:

RefSeq Version	XM_005168905.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168905.3

ORF Insert Sequence:

ATGTTGTCCT ATCTTGCTCG CTGGGTGTCC AGAGGTGCTG TCCAGGCTCT AAAAGGAACA 
AGCAGTCGGC CTCAACTGGT CAAGACACCT CGTATCGGCA ATCAGACTTT GGCAGCGGCT 
CCTCTGCCCT TTGCTGGGGG CAGTCCAGGA GTGAGACAGG CCCGGGCACT GGATCAGGAG 
AGATTACTGC AGATCGACTG GGATGATGGG AGCTGTAGTT TGTATCCATT CACCTGGTTG 
AGGGATAACT GCCAGTGTCC GCTCTGTACG CTGCAGTCGG CTCAAGCACG CAGCCTGCTG 
TTCTCACATC TGGACGTGCA CACCGGGATG GATCACGTAC AACTGATGGA TAATAAGGTG 
TCCATAACTT GGCCTGATCA GCACAGCAGT GAGTTTGATC CTGACTGGCT GAAGAAACGC 
TGCTTTTCCT CTGAGGCCAG ACAGGCTCTG CAGGAGGAGC TCTTCCTTAA TGAGCGTGAG 
TATTGGGACT CTGGTCTCCA GATCCCCACA GCAGATTTCG AGGAGGTTCT ACATGACGAC 
AAGGCTGCAC TGGCCTGGTT AGAAGCTCTG AGACGCATTG GCATAGTCTA CCTGAGGGGG 
GCACCAGCGG AACAGGGCCA GTTGGCCAGA CTCAGCCAGA GAATTGGCTA CCTGCGCCTC 
ACCTTTTATG GACACACGTG GCAAGTGCAG GACAAGCCCA TGGCTAACAA TGTTGCCTAC 
ACTTCTGGAG AACTGAGTCT GCACACAGAT TACCCTGCTC TCCATCATCC ACCTGGGGTG 
CAGTTCCTAC ATTGTGTCCG TCAGGCTGAT GAGGGTGGTG AGAGTGAGGT GGTTGATGGG 
TTTCACATGG CAGAGCAGCT CCGCAGGGAA GATCCAGAGG CGTTTAACAT CCTCTCCTCT 
CTCAGGGTGG ACTTCACAGA CAGCGGTGCT GACTACTGCG ACTTCAGTGT GCAGTCCAAA 
AACCACATTA TAGATGTGGA CTCATCCGGT CGGGTTGTGA GGATCAACTA TAATAATGCC 
ACTCGAGACT CAGTGCTGGA TCTGCCTCTG CATCAGGTTC AGCCCTTCTA CTCGTCTCTG 
AAGGCCTTCG TGGAGCTGCT CAGCCGGCCG GAGAATGTCT TCACTTACAG AATGGAGCCA 
GGTGATGTGG TGACCTTCGA TAACTGGCGT CTGCTGCACG GCCGAAAGAG CTACCAGTCT 
CGAGGGCAGA ACTTAAGACA TCTAGAAGGA GCGTATCTGG ACTGGGACGA GGTCATGTCC 
CGTCTCAGGA TCCTCCGGAA GACGGTCCGC GGAGACAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbox1 ( XM_021477587.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333262.1 Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa13092
Clone ID Related Accession (Same CDS sequence)	XM_005168903.4 , XM_005168904.3 , XM_005168903.3 , XM_005168904.4 , XM_005168905.3 , XM_021477587.1
Accession Version	XM_021477587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	gamma-butyrobetaine dioxygenase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCCT ATCTTGCTCG CTGGGTGTCC AGAGGTGCTG TCCAGGCTCT AAAAGGAACA 
AGCAGTCGGC CTCAACTGGT CAAGACACCT CGTATCGGCA ATCAGACTTT GGCAGCGGCT 
CCTCTGCCCT TTGCTGGGGG CAGTCCAGGA GTGAGACAGG CCCGGGCACT GGATCAGGAG 
AGATTACTGC AGATCGACTG GGATGATGGG AGCTGTAGTT TGTATCCATT CACCTGGTTG 
AGGGATAACT GCCAGTGTCC GCTCTGTACG CTGCAGTCGG CTCAAGCACG CAGCCTGCTG 
TTCTCACATC TGGACGTGCA CACCGGGATG GATCACGTAC AACTGATGGA TAATAAGGTG 
TCCATAACTT GGCCTGATCA GCACAGCAGT GAGTTTGATC CTGACTGGCT GAAGAAACGC 
TGCTTTTCCT CTGAGGCCAG ACAGGCTCTG CAGGAGGAGC TCTTCCTTAA TGAGCGTGAG 
TATTGGGACT CTGGTCTCCA GATCCCCACA GCAGATTTCG AGGAGGTTCT ACATGACGAC 
AAGGCTGCAC TGGCCTGGTT AGAAGCTCTG AGACGCATTG GCATAGTCTA CCTGAGGGGG 
GCACCAGCGG AACAGGGCCA GTTGGCCAGA CTCAGCCAGA GAATTGGCTA CCTGCGCCTC 
ACCTTTTATG GACACACGTG GCAAGTGCAG GACAAGCCCA TGGCTAACAA TGTTGCCTAC 
ACTTCTGGAG AACTGAGTCT GCACACAGAT TACCCTGCTC TCCATCATCC ACCTGGGGTG 
CAGTTCCTAC ATTGTGTCCG TCAGGCTGAT GAGGGTGGTG AGAGTGAGGT GGTTGATGGG 
TTTCACATGG CAGAGCAGCT CCGCAGGGAA GATCCAGAGG CGTTTAACAT CCTCTCCTCT 
CTCAGGGTGG ACTTCACAGA CAGCGGTGCT GACTACTGCG ACTTCAGTGT GCAGTCCAAA 
AACCACATTA TAGATGTGGA CTCATCCGGT CGGGTTGTGA GGATCAACTA TAATAATGCC 
ACTCGAGACT CAGTGCTGGA TCTGCCTCTG CATCAGGTTC AGCCCTTCTA CTCGTCTCTG 
AAGGCCTTCG TGGAGCTGCT CAGCCGGCCG GAGAATGTCT TCACTTACAG AATGGAGCCA 
GGTGATGTGG TGACCTTCGA TAACTGGCGT CTGCTGCACG GCCGAAAGAG CTACCAGTCT 
CGAGGGCAGA ACTTAAGACA TCTAGAAGGA GCGTATCTGG ACTGGGACGA GGTCATGTCC 
CGTCTCAGGA TCCTCCGGAA GACGGTCCGC GGAGACAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333262.1
CDS	268..1569
Translation	MLSYLARWVSRGAVQALKGTSSRPQLVKTPRIGNQTLAAAPLPFAGGSPGVRQARALDQERLLQIDWDDGSCSLYPFTWLRDNCQCPLCTLQSAQARSLLFSHLDVHTGMDHVQLMDNKVSITWPDQHSSEFDPDWLKKRCFSSEARQALQEELFLNEREYWDSGLQIPTADFEEVLHDDKAALAWLEALRRIGIVYLRGAPAEQGQLARLSQRIGYLRLTFYGHTWQVQDKPMANNVAYTSGELSLHTDYPALHHPPGVQFLHCVRQADEGGESEVVDGFHMAEQLRREDPEAFNILSSLRVDFTDSGADYCDFSVQSKNHIIDVDSSGRVVRINYNNATRDSVLDLPLHQVQPFYSSLKAFVELLSRPENVFTYRMEPGDVVTFDNWRLLHGRKSYQSRGQNLRHLEGAYLDWDEVMSRLRILRKTVRGDS

Target ORF information:

RefSeq Version	XM_021477587.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477587.1

ORF Insert Sequence:

ATGTTGTCCT ATCTTGCTCG CTGGGTGTCC AGAGGTGCTG TCCAGGCTCT AAAAGGAACA 
AGCAGTCGGC CTCAACTGGT CAAGACACCT CGTATCGGCA ATCAGACTTT GGCAGCGGCT 
CCTCTGCCCT TTGCTGGGGG CAGTCCAGGA GTGAGACAGG CCCGGGCACT GGATCAGGAG 
AGATTACTGC AGATCGACTG GGATGATGGG AGCTGTAGTT TGTATCCATT CACCTGGTTG 
AGGGATAACT GCCAGTGTCC GCTCTGTACG CTGCAGTCGG CTCAAGCACG CAGCCTGCTG 
TTCTCACATC TGGACGTGCA CACCGGGATG GATCACGTAC AACTGATGGA TAATAAGGTG 
TCCATAACTT GGCCTGATCA GCACAGCAGT GAGTTTGATC CTGACTGGCT GAAGAAACGC 
TGCTTTTCCT CTGAGGCCAG ACAGGCTCTG CAGGAGGAGC TCTTCCTTAA TGAGCGTGAG 
TATTGGGACT CTGGTCTCCA GATCCCCACA GCAGATTTCG AGGAGGTTCT ACATGACGAC 
AAGGCTGCAC TGGCCTGGTT AGAAGCTCTG AGACGCATTG GCATAGTCTA CCTGAGGGGG 
GCACCAGCGG AACAGGGCCA GTTGGCCAGA CTCAGCCAGA GAATTGGCTA CCTGCGCCTC 
ACCTTTTATG GACACACGTG GCAAGTGCAG GACAAGCCCA TGGCTAACAA TGTTGCCTAC 
ACTTCTGGAG AACTGAGTCT GCACACAGAT TACCCTGCTC TCCATCATCC ACCTGGGGTG 
CAGTTCCTAC ATTGTGTCCG TCAGGCTGAT GAGGGTGGTG AGAGTGAGGT GGTTGATGGG 
TTTCACATGG CAGAGCAGCT CCGCAGGGAA GATCCAGAGG CGTTTAACAT CCTCTCCTCT 
CTCAGGGTGG ACTTCACAGA CAGCGGTGCT GACTACTGCG ACTTCAGTGT GCAGTCCAAA 
AACCACATTA TAGATGTGGA CTCATCCGGT CGGGTTGTGA GGATCAACTA TAATAATGCC 
ACTCGAGACT CAGTGCTGGA TCTGCCTCTG CATCAGGTTC AGCCCTTCTA CTCGTCTCTG 
AAGGCCTTCG TGGAGCTGCT CAGCCGGCCG GAGAATGTCT TCACTTACAG AATGGAGCCA 
GGTGATGTGG TGACCTTCGA TAACTGGCGT CTGCTGCACG GCCGAAAGAG CTACCAGTCT 
CGAGGGCAGA ACTTAAGACA TCTAGAAGGA GCGTATCTGG ACTGGGACGA GGTCATGTCC 
CGTCTCAGGA TCCTCCGGAA GACGGTCCGC GGAGACAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbox1 ( XM_017356900.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212389.1 Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X4, mRNA.

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CloneID	ODa59646
Clone ID Related Accession (Same CDS sequence)	XM_017356900.1
Accession Version	XM_017356900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	gamma-butyrobetaine dioxygenase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCCT ATCTTGCTCG CTGGGTGTCC AGAGGTGCTG TCCAGGCTCT AAAAGGAACA 
AGCAGTCGGC CTCAACTGGT CAAGACACCT CGTATCGGCA ATCAGACTTT GGCAGCGGCT 
CCTCTGCCCT TTGCTGGGGG CAGTCCAGGA GTGAGACAGG CCCGGGCACT GGATCAGGAG 
AGATTACTGC AGATCGACTG GGATGATGGG AGCTGTAGTT TGTATCCATT CACCTGGTTG 
AGGGATAACT GCCAGTGTCC GCTCTGTACG CTGCAGTCGG CTCAAGCACG CAGCCTGCTG 
TTCTCACATC TGGACGTGCA CACCGGGATG GATCACGTAC AACTGATGGA TAATAAGGTG 
TCCATAACTT GGCCTGATCA GCACAGCAGT GAGTTTGATC CTGACTGGCT GAAGAAACGC 
TGCTTTTCCT CTGAGGCCAG ACAGGCTCTG CAGGAGGAGC TCTTCCTTAA TGAGCGTGAG 
TATTGGGACT CTGGTCTCCA GATCCCCACA GCAGATTTCG AGGAGGTTCT ACATGACGAC 
AAGGCTGCAC TGGCCTGGTT AGAAGCTCTG AGACGCATTG GCATAGTCTA CCTGAGGGGG 
GCACCAGCGG AACAGGGCCA GTTGGCCAGA CTCAGCCAGA GAATTGGCTA CCTGCGCCTC 
ACCTTTTATG GTGATTCCTT AGTCTTTAAA TTTACTCGCA AGACGGTACT AAATCAAACA 
CACGTGGCAA GTGCAGGACA AGCCCATGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212389.1
CDS	159..911
Translation	MLSYLARWVSRGAVQALKGTSSRPQLVKTPRIGNQTLAAAPLPFAGGSPGVRQARALDQERLLQIDWDDGSCSLYPFTWLRDNCQCPLCTLQSAQARSLLFSHLDVHTGMDHVQLMDNKVSITWPDQHSSEFDPDWLKKRCFSSEARQALQEELFLNEREYWDSGLQIPTADFEEVLHDDKAALAWLEALRRIGIVYLRGAPAEQGQLARLSQRIGYLRLTFYGDSLVFKFTRKTVLNQTHVASAGQAHG

Target ORF information:

RefSeq Version	XM_017356900.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (bbox1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356900.1

ORF Insert Sequence:

ATGTTGTCCT ATCTTGCTCG CTGGGTGTCC AGAGGTGCTG TCCAGGCTCT AAAAGGAACA 
AGCAGTCGGC CTCAACTGGT CAAGACACCT CGTATCGGCA ATCAGACTTT GGCAGCGGCT 
CCTCTGCCCT TTGCTGGGGG CAGTCCAGGA GTGAGACAGG CCCGGGCACT GGATCAGGAG 
AGATTACTGC AGATCGACTG GGATGATGGG AGCTGTAGTT TGTATCCATT CACCTGGTTG 
AGGGATAACT GCCAGTGTCC GCTCTGTACG CTGCAGTCGG CTCAAGCACG CAGCCTGCTG 
TTCTCACATC TGGACGTGCA CACCGGGATG GATCACGTAC AACTGATGGA TAATAAGGTG 
TCCATAACTT GGCCTGATCA GCACAGCAGT GAGTTTGATC CTGACTGGCT GAAGAAACGC 
TGCTTTTCCT CTGAGGCCAG ACAGGCTCTG CAGGAGGAGC TCTTCCTTAA TGAGCGTGAG 
TATTGGGACT CTGGTCTCCA GATCCCCACA GCAGATTTCG AGGAGGTTCT ACATGACGAC 
AAGGCTGCAC TGGCCTGGTT AGAAGCTCTG AGACGCATTG GCATAGTCTA CCTGAGGGGG 
GCACCAGCGG AACAGGGCCA GTTGGCCAGA CTCAGCCAGA GAATTGGCTA CCTGCGCCTC 
ACCTTTTATG GTGATTCCTT AGTCTTTAAA TTTACTCGCA AGACGGTACT AAATCAAACA 
CACGTGGCAA GTGCAGGACA AGCCCATGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibited Carnitine Synthesis Causes Systemic Alteration of Nutrient Metabolism in Zebrafish.
Frontiers in physiology9509(2018)
Li JM,Li LY,Qin X,Degrace P,Demizieux L,Limbu SM,Wang X,Zhang ML,Li DL,Du ZY

Systemic regulation of L-carnitine in nutritional metabolism in zebrafish, Danio rerio.
Scientific reports740815(2017 01)
Li JM,Li LY,Qin X,Ning LJ,Lu DL,Li DL,Zhang ML,Wang X,Du ZY

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Fibin, a novel secreted lateral plate mesoderm signal, is essential for pectoral fin bud initiation in zebrafish.
Developmental biology303(2)527-35(2007 Mar)
Wakahara T,Kusu N,Yamauchi H,Kimura I,Konishi M,Miyake A,Itoh N

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bbox1 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

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Target ORF information:

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