scn4aa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	scn4aa
Entrez Gene ID	572442
Full Name	sodium channel, voltage-gated, type IV, alpha, a
Synonyms	Na_v1.4a,Nav1.4a
General protein information	Preferred Names sodium channel, voltage-gated, type IV, alpha, a Names sodium channel protein type 4 subunit alpha A voltage-gated sodium channel Nav1.4a voltage-gated sodium channel subunit alpha Nav1.4a zscn3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017358480.1	XP_017213969.1	sodium channel protein type 4 subunit alpha A isoform X2
NM_001039825.1	NP_001034914.1	sodium channel protein type 4 subunit alpha A
XM_017358479.1	XP_017213968.1	sodium channel protein type 4 subunit alpha A isoform X1
XM_017358479.2	XP_017213968.1	sodium channel protein type 4 subunit alpha A isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SCN4A	XP_853396.1
Rattus norvegicus (Norway rat)	Scn4a	NP_037310.1
Macaca mulatta (Rhesus monkey)	SCN4A	XP_001116451.1
Mus musculus (house mouse)	Scn4a	NP_573462.2
Xenopus tropicalis (tropical clawed frog)	LOC100492371	XP_002933087.2
Pan troglodytes (chimpanzee)	SCN4A	XP_003315741.1
Homo sapiens (human)	SCN4A	NP_000325.4
Bos taurus (cattle)	SCN4A	XP_003587508.1
Danio rerio (zebrafish)	scn4aa	NP_001034914.1
Gallus gallus (chicken)	SCN4A	XP_004948566.1

Gene Ontology
Bibliography

Related articles in PubMed

Cardiac voltage-gated sodium channel expression and electrophysiological characterization of the sodium current in the zebrafish (Danio rerio) ventricle.
Haverinen J, Hassinen M, Korajoki H, Vornanen M
Progress in biophysics and molecular biology13859-68(2018 10)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

ZBTB42 mutation defines a novel lethal congenital contracture syndrome (LCCS6).
Patel N, Smith LL, Faqeih E, Mohamed J, Gupta VA, Alkuraya FS
Human molecular genetics23(24)6584-93(2014 Dec)

Drug screening in Scn1a zebrafish mutant identifies clemizole as a potential Dravet syndrome treatment.
Baraban SC, Dinday MT, Hortopan GA
Nature communications42410(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following scn4aa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the scn4aa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01341	NM_001039825.1 Latest version!	Danio rerio sodium channel, voltage-gated, type IV, alpha, a (scn4aa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa46304	XM_017358479.2 Latest version!	Danio rerio sodium channel, voltage-gated, type IV, alpha, a (scn4aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa54469	XM_017358480.1 Latest version!	Danio rerio sodium channel, voltage-gated, type IV, alpha, a (scn4aa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
ODa46304	XM_017358479.1	Danio rerio sodium channel, voltage-gated, type IV, alpha, a (scn4aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

scn4aa ( NM_001039825.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001034914.1 Danio rerio sodium channel, voltage-gated, type IV, alpha, a (scn4aa), mRNA.

Price & Availability↑

CloneID	ODa01341
Clone ID Related Accession (Same CDS sequence)	NM_001039825.1
Accession Version	NM_001039825.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554998400000
Organism	Danio rerio(zebrafish)
Product	sodium channel protein type 4 subunit alpha A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ221253.1. On or before Mar 17, 2006 this sequence version replaced XM_696157.1, XM_696159.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA3505381, SAMEA3505388 [ECO:0000350] ##Evidence-Data-END##

ATGGCACGCC TGCTCCCTCC CACAGGCACC AGTGTGTTCC GCCGCTTCAC CCCAGAGTCA 
CTGGTGGAGA TCGAGAGGCT CATCCAGGAG AAGAGCACCA GGGAGGAACT AGAGGGAGCC 
GAGGAGGAAC CACAGGCACC GAGCAGCGAT CTGGAGGCGG GAAAATGCCT GCCGATGATC 
TATGGCGACC CCCCCGGGGA TCTCCTCAAC ACCCCTCTGG AAGACATCGA CCCCTTTTAT 
AAAACACAGA AAACTTTTAT TGTCATAAGC AAGGGAAACA CAATCTTCAG GTTCTCCTCT 
GAACCGGCGA TGTTTTGCAT CAGTCCTTTT AGTATTGTCA GAAGAGGAGC CATCAAAATT 
CTTATACATT CATTATTTAG CATGTTCATC ATGATCACCA TCCTGTCCAA TTGTGTGTTC 
ATGACGATGA GTAACCCTCC AGCCTGGAGT AAAACAGTCG AGTATGTTTT TACTGGTATA 
TATACCTTTG AAGCGACTGT CAAAGTTTTA TCCCGAGGCT TCTGCATTGG ACCTTTTACA 
TTCCTCCGTG ATCCATGGAA CTGGCTTGAT TTCATGGTGA TCAGCATGGC GTACGTCACA 
GAGTTTGTAG ACCTTGGCAA TGTGTCAGCA CTGAGGACAT TTCGTGTGCT TCGAGCTCTC 
AAAACAATCA CAGTTATTCC TGGTTTGAAA ACCATTGTTG GGGCTTTGAT TCAATCTGTC 
AAGAAGATGA TAGACGTCAT GATTTTGACC ATCTTTGCAC TGGCTGTATT TGCCCTCATT 
GGACTACAGC TGTTCATGGG AAATCTGCGC CAAAAGTGCA TCCGTTGGCC CATTCTCAAC 
AGCACCATCT TTGACGTCTA CAACTCCAAC ATGGTCAATG ACACCACCCT CAACGTTACC 
GATACTTTCG ATTTCAAAGC TTACATAAAC AATGAAGAAA ACCAATACTT TCTGGAAGGA 
AGTTTAGATG CTTTACTATG TGGCAACAGT TCTGATGCTG GGAGATGTCC AGAAGGATAC 
ACGTGTATGA AAGCTGGTCG AAACCCAAAC TATGGCTATA CAAGCTATGA CAACTTTGGA 
TGGGCCTTTC TCGCCCTCTT CAGACTCATG ACCCAAGACT TTTGGGAGAA TCTTTTCCAG 
CTGACCCTTC GTGCTGCTGG GAAGACTTAC ATGATCTTCT TCGTGGTGGT CATCTTCCTG 
GGCTCTTTCT ACCTCATCAA TCTGATCTTG GCTGTGGTTG CAATGGCATA CGACGAGCAG 
AATGAGGCCA CATTGGCCGA GGCTCGTGAC AAAGAAGAGG AGTTTCAAAG ATTACTTGAG 
CAGCTCAAGA ACCAAGAGAC AGGCAGTAAA GCATCTTTGG CCAGCCAGAA AACACAAAGT 
CGAGGGTCAA ACCGGACCGG TTCCCTGCAC GATCTTGCAG ATGAAGATGT CATAAAGGAC 
TGTAATGGCC GAATAGTACC TCGCCTTATT GTCAACAGAG TTTCCTCTAA CAAAGAGTTA 
TCAGCAGAAG AGGACCAGAA GTCTCTGAGT TCAAAACACA GCATGCAGTA CTTGGACCAG 
CCCAAACTCT CCAAGAGAAC AGCTAGTGCT CTCAGTGTAC TCACTGCAAC TATGGAGGGG 
TTGGAGGATG CCCAGAGGCC TTGTCCCCCT GGCTGGTATA AGTTTGCGGA TATGTTCCTT 
AAATGGGACT GCTGCGCTCC CTGGATCTTG TTTAAGAAGT GGGTTCATTT TGTAGTGATG 
GACCCATTCG TTGACCTGGG TATCACCATC TGCATTGTGC TCAATACACT GTTCATGGCC 
ATGGAGCATT ACCCTATGAG TCCACATTTT GAACATGTCC TCTCAGTGGG CAACTTGGTG 
TTCACAGGAA TCTTCACAGC TGAAATGGTG TTCAAGCTTA TAGCTATGGA CCCTTACTAC 
TACTTCCAGG TGGGCTGGAA CATTTTTGAC AGCATCATTG TCACACTCAG CCTGGTGGAG 
TTGGGACTGG CCAACGTTCA GGGATTGTCC GTTCTAAGGT CCTTTCGTTT GCTACGTGTC 
TTCAAACTGG CTAAATCTTG GCCCACCCTT AACATGCTGA TCAAGATCAT CGGCAACTCA 
GTGGGTGCTC TAGGGAACCT AACACTTGTT CTGGCCATCA TTGTCTTCAT CTTTGCCGTG 
GTGGGCATGC AGCTTTTTGG AAAAAGCTAC AAGGACTGCG TTTGTAAGAT TTCTGAGGAT 
TGCGAGCTGC CCCGCTGGCA CATGAACGAC TTCTTCCACT CATTCCTCAT CGTCTTTCGG 
ATCTTATGTG GAGAGTGGAT TGAGACTATG TGGGACTGCA TGGAGGTGGC AGGAGCTAGC 
ATGTGTTTGA TAGTCTTCAT GATGGTCATG GTCATCGGAA ACCTTGTGGT GCTGAATCTG 
TTTCTGGCCC TGCTGCTTAG CTCCTTCAGT GGAGATAACC TGTCTGGAGG TGATGATGAT 
GGAGAGATGA ACAACCTTCA GATTGCCATT GGCCGCATCA CCAGGGGTAT CGATTGGGTT 
AAAGCCTTAG TGGCCAGTAT GGTGCAACGG ATTCTGGGAA AGAAACCTGA TAATACCAAA 
GAGGAAGGGG AGGGAGACAT AGAACTGTAC GCTTTAAATC ACCTTGATGA AGGAAAAATG 
GCAGATGGTT TGACCAACTG TTTGTCTCCA ACATTGACTG TACCTATTGC TCGTTGTGAG 
TCTGATGTGG AGGAAGATGA GGACTCTGAA TCTTCTGATG AGGAAGACGC AAAGGCCACT 
CTTAATGATG GTGACTCTTC GGTTTGTAGC ACAGTGGACT ACCAGCCTCC AGAACCTGAG 
CCTGAACCTG AAGAGGTAGA AGAAGAGGAA CCAGAACCTG AAGAACCAGA GGCCTGTTTC 
ACAGAGGGCT GTATTAGGCG ATGTGCATGT TTGAGTGTTG ACATCACAGA AGGATGGGGT 
AAAAAATGGT GGAACCTCAG AAGGACATGC TTCACCATCG TTGAGCATGA TTACTTTGAG 
ACCTTCATCA TCTTTATGAT CCTCCTTAGC AGTGGAGCAC TGGCTTTTGA GGATATCAAC 
ATTGAGAGGC GCAGAGTGAT CAAGACCATT CTGGAGTATG CTGATAAAGT CTTTACATAT 
ATTTTTATAG TGGAGATGTT ACTGAAGTGG GTGGCATATG GCTTCAAGAC CTACTTCACT 
AATGCATGGT GCTGGCTGGA CTTCCTCATT GTGGATGTGT CTCTGGTCAG TTTAACGGCT 
AATTTAATGG GCTACTCTGA GCTGGGGGCA ATCAAATCTC TCAGGACACT TAGAGCTCTT 
CGTCCACTTC GAGCCCTATC CAGATTTGAA GGAATGAGGG TGGTAGTGAA TGCACTTGTA 
GGTGCCATTC CCTCTATTTT TAACGTGCTC CTGGTGTGTC TGATATTCTG GCTCATCTTC 
AGCATTATGG GGGTCAATCT GTTTGCCGGA AAATTCTACC ACTGCATCAA CACCACCACA 
GAGGAACGGA TCCCCATGGA TGTAGTCAAC AACAAGAGTG ACTGCATGGC ACTGATGTAC 
ACCAACGAGG TGCGATGGGT CAATGTCAAG GTCAACTACG ACAACGTGGG ACTCGGCTAC 
CTCTCTCTGC TGCAGATTGC CACATTCAAA GGCTGGATGG ATATCATGTA TGCTGCAGTG 
GATTCTAGAG AGGTGGATGA GCAGCCATCA TATGAAATCA ACCTTTACAT GTACCTTTAT 
TTTGTTATTT TCATCATTTT TGGCTCCTTT TTTACTCTCA ACCTCTTTAT TGGTGTCATC 
ATTGACAACT TCAATCAGCA AAAATCAAAG TTTGGAGGGA AAGACATTTT CATGACTGAG 
GAACAGAAAA AGTATTACAA TGCCATGAAG AAGCTGGGTG CAAAGAAACG TCCAAAACCT 
ATACCTCGAC CATCAAATAT TATCCAGGGT TTGGTGTTTG ACTTCATATC AAAACAGTTC 
TTTGACATTT TTATCATGGT GCTAATCTGC CTCAACATGG TGACCATGAT GATAGAGACG 
GATGACCAGA GTGCTGAGAA AGAATATGTC CTGTACCAGA TCAATCTGGT CTTCATCGTC 
GTCTTCACAA GTGAATGTGT ACTTAAATTA TTTGCACTCA GACAGTACTT TTTCACTATT 
GGCTGGAACG TCTTTGATTT TGTTGTAGTC ATCCTTTCTA TTGCAGGTCT AATGTTATCA 
GACATCATTG AGAAGTACTT TGTGTCTCCT ACTTTGTTTC GTGTCATCAG ACTTGCAAGA 
ATCGGCCGTG TGCTGCGTCT CATCAGAGGG GCGAAGGGCA TTCGGACCCT TTTGTTTGCC 
TTGATGATGT CACTTCCTGC CCTTTTCAAC ATTGGACTGC TTCTGTTCCT GATCATGTTC 
ATCTTCTCCA TCTTTGGCAT GTCTAACTTT GCCTATGTGA AAAAGCAGGC AGGCATCGAT 
GACATCTTCA ACTTTGAAAC ATTTGGTGGC AGCATTATCT GCCTGTTTGA GATCACAACC 
TCGGCAGGCT GGGACGGCCT TCTGCTGCCT ATTCTAAACA GTGGCCCTCC TGATTGTGAC 
CCTGACTTTG AAAACCCTGG TACAGATGTG CGGGGAAACT GCGGGAATCC TGGCATGGGC 
ATCATGTTTT TCTGCAGTTA CATCATCATG TCATTCCTGG TGGTCGTGAA CATGTACATC 
GCCATCATTC TGGAGAACTT CAACAATGCT CAAGAGGAAA GTGGAGACCC GCTCTGTGAG 
GATGACTTTG ATATGTTTGA TGAAACATGG GAAAAGTTTG ACGTCGATGC TACGCAGTTT 
ATTGAATACG ACCGCTTGTT TGACTTTGTC GATGCCCTGC AGGAGCCTTT ACGAATTGCC 
AAACCCAACC GTCTCAAGCT GATCTCCATG GACATCCCCA TTGTAAACGG CGACAAAATC 
CACTCTCAGG ATATACTTTT AGCAGTGACC CGGGAAGTAC TTGGCGACAC TATTGAGATG 
GATGCCATGA AGGAGAGCAT CGAAGCCAAG TTCATCATGA ACAACCCCAC ATCTGCCTCG 
TTTGAGCCAA TCATCACCAC TCTCAGAAGG AAGGAGGAAG AGCGAGCAGC CATTGCGGTT 
CAAAGAATAT ACCGTAGGCA TCTTCTCAAG CGTGCCATCC GCTATGCCTG CTTCATGCGC 
CGTTCAAAGA GGAAAGTTAG GAATCCCAAC GACAATGAAC CTCCTGAAAC TGAAGGGTTG 
ATTGCAAGGA AGATGAATAC CCTATATGGC AGCAATCCAG AACTTGCCAT GGCTCTTGAA 
TTGGAGACTA GGCCAATGCG GCCAAATTCA CAGCCTCCTA AACCAAGCCA GGTCACACAG 
ACCAGAGCAT CAGTGACCTT CCCTAGGCCG CAAGGACAGC TCATCCTCCC AGTGGAGCTC 
ACGAGTGAGG TGATCCTCCG GTCTGCACCC ATTACACACA GCCTCAACAG TTCAGAGAAT 
GCAACAACGA TAAAGGAGTC AATAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034914.1
CDS	1..5490
Translation	MARLLPPTGTSVFRRFTPESLVEIERLIQEKSTREELEGAEEEPQAPSSDLEAGKCLPMIYGDPPGDLLNTPLEDIDPFYKTQKTFIVISKGNTIFRFSSEPAMFCISPFSIVRRGAIKILIHSLFSMFIMITILSNCVFMTMSNPPAWSKTVEYVFTGIYTFEATVKVLSRGFCIGPFTFLRDPWNWLDFMVISMAYVTEFVDLGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKMIDVMILTIFALAVFALIGLQLFMGNLRQKCIRWPILNSTIFDVYNSNMVNDTTLNVTDTFDFKAYINNEENQYFLEGSLDALLCGNSSDAGRCPEGYTCMKAGRNPNYGYTSYDNFGWAFLALFRLMTQDFWENLFQLTLRAAGKTYMIFFVVVIFLGSFYLINLILAVVAMAYDEQNEATLAEARDKEEEFQRLLEQLKNQETGSKASLASQKTQSRGSNRTGSLHDLADEDVIKDCNGRIVPRLIVNRVSSNKELSAEEDQKSLSSKHSMQYLDQPKLSKRTASALSVLTATMEGLEDAQRPCPPGWYKFADMFLKWDCCAPWILFKKWVHFVVMDPFVDLGITICIVLNTLFMAMEHYPMSPHFEHVLSVGNLVFTGIFTAEMVFKLIAMDPYYYFQVGWNIFDSIIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKISEDCELPRWHMNDFFHSFLIVFRILCGEWIETMWDCMEVAGASMCLIVFMMVMVIGNLVVLNLFLALLLSSFSGDNLSGGDDDGEMNNLQIAIGRITRGIDWVKALVASMVQRILGKKPDNTKEEGEGDIELYALNHLDEGKMADGLTNCLSPTLTVPIARCESDVEEDEDSESSDEEDAKATLNDGDSSVCSTVDYQPPEPEPEPEEVEEEEPEPEEPEACFTEGCIRRCACLSVDITEGWGKKWWNLRRTCFTIVEHDYFETFIIFMILLSSGALAFEDINIERRRVIKTILEYADKVFTYIFIVEMLLKWVAYGFKTYFTNAWCWLDFLIVDVSLVSLTANLMGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIFNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTEERIPMDVVNNKSDCMALMYTNEVRWVNVKVNYDNVGLGYLSLLQIATFKGWMDIMYAAVDSREVDEQPSYEINLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKSKFGGKDIFMTEEQKKYYNAMKKLGAKKRPKPIPRPSNIIQGLVFDFISKQFFDIFIMVLICLNMVTMMIETDDQSAEKEYVLYQINLVFIVVFTSECVLKLFALRQYFFTIGWNVFDFVVVILSIAGLMLSDIIEKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLIMFIFSIFGMSNFAYVKKQAGIDDIFNFETFGGSIICLFEITTSAGWDGLLLPILNSGPPDCDPDFENPGTDVRGNCGNPGMGIMFFCSYIIMSFLVVVNMYIAIILENFNNAQEESGDPLCEDDFDMFDETWEKFDVDATQFIEYDRLFDFVDALQEPLRIAKPNRLKLISMDIPIVNGDKIHSQDILLAVTREVLGDTIEMDAMKESIEAKFIMNNPTSASFEPIITTLRRKEEERAAIAVQRIYRRHLLKRAIRYACFMRRSKRKVRNPNDNEPPETEGLIARKMNTLYGSNPELAMALELETRPMRPNSQPPKPSQVTQTRASVTFPRPQGQLILPVELTSEVILRSAPITHSLNSSENATTIKESIV

Target ORF information:

RefSeq Version	NM_001039825.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sodium channel, voltage-gated, type IV, alpha, a (scn4aa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039825.1

ORF Insert Sequence:

ATGGCACGCC TGCTCCCTCC CACAGGCACC AGTGTGTTCC GCCGCTTCAC CCCAGAGTCA 
CTGGTGGAGA TCGAGAGGCT CATCCAGGAG AAGAGCACCA GGGAGGAACT AGAGGGAGCC 
GAGGAGGAAC CACAGGCACC GAGCAGCGAT CTGGAGGCGG GAAAATGCCT GCCGATGATC 
TATGGCGACC CCCCCGGGGA TCTCCTCAAC ACCCCTCTGG AAGACATCGA CCCCTTTTAT 
AAAACACAGA AAACTTTTAT TGTCATAAGC AAGGGAAACA CAATCTTCAG GTTCTCCTCT 
GAACCGGCGA TGTTTTGCAT CAGTCCTTTT AGTATTGTCA GAAGAGGAGC CATCAAAATT 
CTTATACATT CATTATTTAG CATGTTCATC ATGATCACCA TCCTGTCCAA TTGTGTGTTC 
ATGACGATGA GTAACCCTCC AGCCTGGAGT AAAACAGTCG AGTATGTTTT TACTGGTATA 
TATACCTTTG AAGCGACTGT CAAAGTTTTA TCCCGAGGCT TCTGCATTGG ACCTTTTACA 
TTCCTCCGTG ATCCATGGAA CTGGCTTGAT TTCATGGTGA TCAGCATGGC GTACGTCACA 
GAGTTTGTAG ACCTTGGCAA TGTGTCAGCA CTGAGGACAT TTCGTGTGCT TCGAGCTCTC 
AAAACAATCA CAGTTATTCC TGGTTTGAAA ACCATTGTTG GGGCTTTGAT TCAATCTGTC 
AAGAAGATGA TAGACGTCAT GATTTTGACC ATCTTTGCAC TGGCTGTATT TGCCCTCATT 
GGACTACAGC TGTTCATGGG AAATCTGCGC CAAAAGTGCA TCCGTTGGCC CATTCTCAAC 
AGCACCATCT TTGACGTCTA CAACTCCAAC ATGGTCAATG ACACCACCCT CAACGTTACC 
GATACTTTCG ATTTCAAAGC TTACATAAAC AATGAAGAAA ACCAATACTT TCTGGAAGGA 
AGTTTAGATG CTTTACTATG TGGCAACAGT TCTGATGCTG GGAGATGTCC AGAAGGATAC 
ACGTGTATGA AAGCTGGTCG AAACCCAAAC TATGGCTATA CAAGCTATGA CAACTTTGGA 
TGGGCCTTTC TCGCCCTCTT CAGACTCATG ACCCAAGACT TTTGGGAGAA TCTTTTCCAG 
CTGACCCTTC GTGCTGCTGG GAAGACTTAC ATGATCTTCT TCGTGGTGGT CATCTTCCTG 
GGCTCTTTCT ACCTCATCAA TCTGATCTTG GCTGTGGTTG CAATGGCATA CGACGAGCAG 
AATGAGGCCA CATTGGCCGA GGCTCGTGAC AAAGAAGAGG AGTTTCAAAG ATTACTTGAG 
CAGCTCAAGA ACCAAGAGAC AGGCAGTAAA GCATCTTTGG CCAGCCAGAA AACACAAAGT 
CGAGGGTCAA ACCGGACCGG TTCCCTGCAC GATCTTGCAG ATGAAGATGT CATAAAGGAC 
TGTAATGGCC GAATAGTACC TCGCCTTATT GTCAACAGAG TTTCCTCTAA CAAAGAGTTA 
TCAGCAGAAG AGGACCAGAA GTCTCTGAGT TCAAAACACA GCATGCAGTA CTTGGACCAG 
CCCAAACTCT CCAAGAGAAC AGCTAGTGCT CTCAGTGTAC TCACTGCAAC TATGGAGGGG 
TTGGAGGATG CCCAGAGGCC TTGTCCCCCT GGCTGGTATA AGTTTGCGGA TATGTTCCTT 
AAATGGGACT GCTGCGCTCC CTGGATCTTG TTTAAGAAGT GGGTTCATTT TGTAGTGATG 
GACCCATTCG TTGACCTGGG TATCACCATC TGCATTGTGC TCAATACACT GTTCATGGCC 
ATGGAGCATT ACCCTATGAG TCCACATTTT GAACATGTCC TCTCAGTGGG CAACTTGGTG 
TTCACAGGAA TCTTCACAGC TGAAATGGTG TTCAAGCTTA TAGCTATGGA CCCTTACTAC 
TACTTCCAGG TGGGCTGGAA CATTTTTGAC AGCATCATTG TCACACTCAG CCTGGTGGAG 
TTGGGACTGG CCAACGTTCA GGGATTGTCC GTTCTAAGGT CCTTTCGTTT GCTACGTGTC 
TTCAAACTGG CTAAATCTTG GCCCACCCTT AACATGCTGA TCAAGATCAT CGGCAACTCA 
GTGGGTGCTC TAGGGAACCT AACACTTGTT CTGGCCATCA TTGTCTTCAT CTTTGCCGTG 
GTGGGCATGC AGCTTTTTGG AAAAAGCTAC AAGGACTGCG TTTGTAAGAT TTCTGAGGAT 
TGCGAGCTGC CCCGCTGGCA CATGAACGAC TTCTTCCACT CATTCCTCAT CGTCTTTCGG 
ATCTTATGTG GAGAGTGGAT TGAGACTATG TGGGACTGCA TGGAGGTGGC AGGAGCTAGC 
ATGTGTTTGA TAGTCTTCAT GATGGTCATG GTCATCGGAA ACCTTGTGGT GCTGAATCTG 
TTTCTGGCCC TGCTGCTTAG CTCCTTCAGT GGAGATAACC TGTCTGGAGG TGATGATGAT 
GGAGAGATGA ACAACCTTCA GATTGCCATT GGCCGCATCA CCAGGGGTAT CGATTGGGTT 
AAAGCCTTAG TGGCCAGTAT GGTGCAACGG ATTCTGGGAA AGAAACCTGA TAATACCAAA 
GAGGAAGGGG AGGGAGACAT AGAACTGTAC GCTTTAAATC ACCTTGATGA AGGAAAAATG 
GCAGATGGTT TGACCAACTG TTTGTCTCCA ACATTGACTG TACCTATTGC TCGTTGTGAG 
TCTGATGTGG AGGAAGATGA GGACTCTGAA TCTTCTGATG AGGAAGACGC AAAGGCCACT 
CTTAATGATG GTGACTCTTC GGTTTGTAGC ACAGTGGACT ACCAGCCTCC AGAACCTGAG 
CCTGAACCTG AAGAGGTAGA AGAAGAGGAA CCAGAACCTG AAGAACCAGA GGCCTGTTTC 
ACAGAGGGCT GTATTAGGCG ATGTGCATGT TTGAGTGTTG ACATCACAGA AGGATGGGGT 
AAAAAATGGT GGAACCTCAG AAGGACATGC TTCACCATCG TTGAGCATGA TTACTTTGAG 
ACCTTCATCA TCTTTATGAT CCTCCTTAGC AGTGGAGCAC TGGCTTTTGA GGATATCAAC 
ATTGAGAGGC GCAGAGTGAT CAAGACCATT CTGGAGTATG CTGATAAAGT CTTTACATAT 
ATTTTTATAG TGGAGATGTT ACTGAAGTGG GTGGCATATG GCTTCAAGAC CTACTTCACT 
AATGCATGGT GCTGGCTGGA CTTCCTCATT GTGGATGTGT CTCTGGTCAG TTTAACGGCT 
AATTTAATGG GCTACTCTGA GCTGGGGGCA ATCAAATCTC TCAGGACACT TAGAGCTCTT 
CGTCCACTTC GAGCCCTATC CAGATTTGAA GGAATGAGGG TGGTAGTGAA TGCACTTGTA 
GGTGCCATTC CCTCTATTTT TAACGTGCTC CTGGTGTGTC TGATATTCTG GCTCATCTTC 
AGCATTATGG GGGTCAATCT GTTTGCCGGA AAATTCTACC ACTGCATCAA CACCACCACA 
GAGGAACGGA TCCCCATGGA TGTAGTCAAC AACAAGAGTG ACTGCATGGC ACTGATGTAC 
ACCAACGAGG TGCGATGGGT CAATGTCAAG GTCAACTACG ACAACGTGGG ACTCGGCTAC 
CTCTCTCTGC TGCAGATTGC CACATTCAAA GGCTGGATGG ATATCATGTA TGCTGCAGTG 
GATTCTAGAG AGGTGGATGA GCAGCCATCA TATGAAATCA ACCTTTACAT GTACCTTTAT 
TTTGTTATTT TCATCATTTT TGGCTCCTTT TTTACTCTCA ACCTCTTTAT TGGTGTCATC 
ATTGACAACT TCAATCAGCA AAAATCAAAG TTTGGAGGGA AAGACATTTT CATGACTGAG 
GAACAGAAAA AGTATTACAA TGCCATGAAG AAGCTGGGTG CAAAGAAACG TCCAAAACCT 
ATACCTCGAC CATCAAATAT TATCCAGGGT TTGGTGTTTG ACTTCATATC AAAACAGTTC 
TTTGACATTT TTATCATGGT GCTAATCTGC CTCAACATGG TGACCATGAT GATAGAGACG 
GATGACCAGA GTGCTGAGAA AGAATATGTC CTGTACCAGA TCAATCTGGT CTTCATCGTC 
GTCTTCACAA GTGAATGTGT ACTTAAATTA TTTGCACTCA GACAGTACTT TTTCACTATT 
GGCTGGAACG TCTTTGATTT TGTTGTAGTC ATCCTTTCTA TTGCAGGTCT AATGTTATCA 
GACATCATTG AGAAGTACTT TGTGTCTCCT ACTTTGTTTC GTGTCATCAG ACTTGCAAGA 
ATCGGCCGTG TGCTGCGTCT CATCAGAGGG GCGAAGGGCA TTCGGACCCT TTTGTTTGCC 
TTGATGATGT CACTTCCTGC CCTTTTCAAC ATTGGACTGC TTCTGTTCCT GATCATGTTC 
ATCTTCTCCA TCTTTGGCAT GTCTAACTTT GCCTATGTGA AAAAGCAGGC AGGCATCGAT 
GACATCTTCA ACTTTGAAAC ATTTGGTGGC AGCATTATCT GCCTGTTTGA GATCACAACC 
TCGGCAGGCT GGGACGGCCT TCTGCTGCCT ATTCTAAACA GTGGCCCTCC TGATTGTGAC 
CCTGACTTTG AAAACCCTGG TACAGATGTG CGGGGAAACT GCGGGAATCC TGGCATGGGC 
ATCATGTTTT TCTGCAGTTA CATCATCATG TCATTCCTGG TGGTCGTGAA CATGTACATC 
GCCATCATTC TGGAGAACTT CAACAATGCT CAAGAGGAAA GTGGAGACCC GCTCTGTGAG 
GATGACTTTG ATATGTTTGA TGAAACATGG GAAAAGTTTG ACGTCGATGC TACGCAGTTT 
ATTGAATACG ACCGCTTGTT TGACTTTGTC GATGCCCTGC AGGAGCCTTT ACGAATTGCC 
AAACCCAACC GTCTCAAGCT GATCTCCATG GACATCCCCA TTGTAAACGG CGACAAAATC 
CACTCTCAGG ATATACTTTT AGCAGTGACC CGGGAAGTAC TTGGCGACAC TATTGAGATG 
GATGCCATGA AGGAGAGCAT CGAAGCCAAG TTCATCATGA ACAACCCCAC ATCTGCCTCG 
TTTGAGCCAA TCATCACCAC TCTCAGAAGG AAGGAGGAAG AGCGAGCAGC CATTGCGGTT 
CAAAGAATAT ACCGTAGGCA TCTTCTCAAG CGTGCCATCC GCTATGCCTG CTTCATGCGC 
CGTTCAAAGA GGAAAGTTAG GAATCCCAAC GACAATGAAC CTCCTGAAAC TGAAGGGTTG 
ATTGCAAGGA AGATGAATAC CCTATATGGC AGCAATCCAG AACTTGCCAT GGCTCTTGAA 
TTGGAGACTA GGCCAATGCG GCCAAATTCA CAGCCTCCTA AACCAAGCCA GGTCACACAG 
ACCAGAGCAT CAGTGACCTT CCCTAGGCCG CAAGGACAGC TCATCCTCCC AGTGGAGCTC 
ACGAGTGAGG TGATCCTCCG GTCTGCACCC ATTACACACA GCCTCAACAG TTCAGAGAAT 
GCAACAACGA TAAAGGAGTC AATAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn4aa ( XM_017358479.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213968.1 Danio rerio sodium channel, voltage-gated, type IV, alpha, a (scn4aa), transcript variant X1, mRNA.

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CloneID	ODa46304
Clone ID Related Accession (Same CDS sequence)	XM_017358479.1 , XM_017358479.2
Accession Version	XM_017358479.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	sodium channel protein type 4 subunit alpha A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACGCC TGCTCCCTCC CACAGGCACC AGTGTGTTCC GCCGCTTCAC CCCAGAGTCA 
CTGGTGGAGA TCGAGAGGCT CATCCAGGAG AAGAGCACCA GGGAGGAACT AGAGGGAGCC 
GAGGAGGAAC CACAGGCACC GAGCAGCGAT CTGGAGGCGG GAAAATGCCT GCCGATGATC 
TATGGCGACC CCCCCGGGGA TCTCCTCAAC ACCCCTCTGG AAGACATCGA CCCCTTTTAT 
AAAACACAGA AAACTTTTAT TGTCATAAGC AAGGGAAACA CAATCTTCAG GTTCTCTTCT 
GAACCGGCGA TGTTTTGCAT CAGTCCTTTT AGTATTGTCA GAAGAGGAGC CATCAAAATT 
CTTATACATT CGTTATTTAG CATGTTCATC ATGATCACCA TCCTGTCCAA TTGCGTGTTC 
ATGACGATGA GTAACCCTCC AGCCTGGAGT AAAACTGTCG AGTATGTTTT TACTGGTATA 
TATACCTTTG AAGCGACTGT CAAAGTTTTA TCCCGAGGCT TCTGCATTGG ACCTTTTACA 
TTCCTCCGTG ATCCATGGAA CTGGCTTGAT TTCATGGTGA TCAGCATGGC GTACGTCACA 
GAGTTTGTAG ACCTTGGCAA TGTGTCAGCA CTGAGGACAT TTCGTGTGCT TCGAGCTCTC 
AAAACAATCA CAGTTATTCC TGGTTTGAAA ACCATTGTTG GGGCTTTGAT TCAATCTGTC 
AAGAAGATGA TAGACGTCAT GATTTTGACC ATCTTTGCAC TGGCTGTATT TGCCCTCATT 
GGACTACAGC TGTTCATGGG AAATCTGCGC CAAAAGTGCA TCCGTTGGCC CATTCTCAAC 
AGCACCATCT TTGACGTCTA CAACTCCAAC ATGGTCAATG ACACCACCCT CAACGTTACC 
GATACTTTCG ATTTCAAAGC TTACATAAGC AATGAAGAAA ACCAATACTT TCTGGAAGGA 
AGTTTAGATG CTTTACTATG TGGCAACAGT TCTGATGCTG GGAGATGTCC AGAAGGATAC 
ACGTGTATGA AAGCTGGTCG AAACCCAAAC TATGGCTATA CAAGCTATGA CAACTTTGGA 
TGGGCCTTTC TCGCCCTCTT CAGACTCATG ACCCAGGACT TTTGGGAGAA TCTTTTCCAG 
CTGACCCTTC GTGCTGCTGG GAAGACTTAC ATGATCTTCT TCGTGGTGGT CATCTTCCTG 
GGCTCTTTCT ACCTCATCAA TCTGATCTTG GCTGTGGTTG CAATGGCATA CGACGAGCAG 
AATGAGGCCA CATTGGCCGA GGCTCGTGAC AAAGAAGAGG AGTTTCAAAG ATTACTTGAG 
CAGCTCAAGA ACCAAGAGAC AGGCAGTAAA GCATCTTTGG CCAGCCAGAA AACACAAAGT 
CGAGGGTCAA ACCGGACAGG TTCCCTGCAC GATCTTGCAG ATGAAGATGT CATAAAGGAC 
TGTAATGGCC GAATAGTACC TCGCCTTATT GTCAACAGAG TTTCCTCTAA CAAAGAGTTA 
TCAGCAGAAG AGGACCAGAA GTCTCTGAGT TCAAAACACA GCATGCAGTA CTTGGACCAG 
CCCAAACTCT CCAAGAGAAC AGCTAGTGCT CTCAGTGTAC TCACTGCAAC TATGGAGGGG 
TTGGAGGATG CCCAGAGGCC TTGTCCCCCT GGCTGGTATA AGTTTGCGGA TATGTTCCTT 
AAATGGGACT GCTGCGCTCC CTGGATCTTG TTTAAGAAGT GGGTTCATTT TGTAGTGATG 
GACCCATTCG TTGACCTGGG TATCACCATC TGCATTGTGC TCAATACACT GTTCATGGCC 
ATGGAGCATT ACCCTATGAG TCCACATTTT GAACATGTCC TCTCAGTGGG CAACTTGGTG 
TTCACAGGAA TCTTCACAGC TGAAATGGTG TTCAAGCTTA TAGCTATGGA CCCTTACTAC 
TACTTCCAGG TGGGCTGGAA CATTTTTGAC AGCATCATTG TCACACTCAG CCTGGTGGAG 
TTGGGACTGG CCAACGTTCA GGGATTGTCC GTTCTAAGGT CCTTTCGTTT GCTACGTGTC 
TTCAAACTGG CTAAATCTTG GCCCACCCTT AACATGCTGA TCAAGATCAT CGGCAACTCA 
GTGGGTGCTC TAGGGAACCT AACACTTGTT CTGGCCATCA TTGTCTTCAT CTTTGCCGTG 
GTGGGCATGC AGCTTTTTGG AAAAAGCTAC AAGGACTGCG TTTGTAAGAT CTCTGAGGAT 
TGCGAGCTGC CCCGCTGGCA CATGAACGAC TTCTTCCACT CATTCCTCAT CGTCTTTCGG 
ATCTTATGTG GAGAGTGGAT TGAGACTATG TGGGACTGCA TGGAGGTGGC AGGAGCTAGC 
ATGTGTTTGA TAGTCTTCAT GATGGTCATG GTCATCGGAA ACCTTGTGGT GCTGAATCTG 
TTTCTGGCCC TGCTGCTTAG CTCCTTCAGT GGAGATAACC TGTCTGGAGG TGATGATGAT 
GGAGAGATGA ACAACCTTCA GATTGCCATT GGCCGCATCA CCAGGGGTAT CGATTGGGTT 
AAAGCCTTAG TTGCCAGTAT GGTGCAACGG ATTCTGGGAA AGAAACCTGA TAATACCAAA 
GAGGAAGGGG AGGGAGACAT AGAACTGTAC GCTTTAAATC ACCTTGATGA AGGAAAAATG 
GCAGATGGTT TGACCAACTG TTTGTCTCCA ACATTGACTG TACCTATTGC TCGTTGTGAG 
TCTGATGTGG AGGAAGATGA GGACTCTGAA TCTTCTGATG AGGAAGACGC AAAGGCCACT 
CTTAATGATG GTGACTCTTC GGTTTGTAGC ACAGTGGACT ACCAGCCTCC AGAACCTGAG 
CCTGAACCTG AAGAGGTAGA AGAAGAGGAA CCAGAACCTG AAGAACCAGA GGCCTGTTTC 
ACAGAGGGCT GTATTAGGCG ATGTGCATGT TTGAGTGTTG ACATCACAGA AGGATGGGGT 
AAAAAATGGT GGAACCTCAG AAGGACATGC TTCACCATCG TTGAGCATGA TTACTTTGAG 
ACCTTCATCA TCTTTATGAT CCTCCTTAGC AGTGGAGCAC TGGCTTTTGA GGATATCAAC 
ATTGAGAGGC GCAGAGTGAT CAAGACCATT CTGGAGTATG CTGATAAAGT CTTTACATAT 
ATTTTTATAG TGGAGATGTT ACTGAAGTGG GTGGCATATG GCTTCAAGAC CTACTTCACT 
AATGCATGGT GCTGGCTGGA CTTCCTCATT GTGGATGTGT CTCTGGTCAG TTTAACGGCT 
AATTTAATGG GCTACTCTGA GCTGGGGGCA ATCAAATCTC TCAGGACACT TAGAGCTCTT 
CGTCCACTTC GAGCCCTATC CAGATTTGAA GGAATGAGGG TGGTAGTGAA TGCACTTGTA 
GGTGCCATTC CCTCTATTTT TAACGTGCTC CTGGTGTGTC TGATATTCTG GCTCATCTTC 
AGCATTATGG GGGTCAATCT GTTTGCCGGA AAATTCTACC ACTGCATCAA CACCACCACA 
GAGGAACGGA TCCCCATGGA TGTAGTCAAC AACAAGAGTG ACTGCATGGC ACTGATGTAC 
ACCAACGAGG TGCGATGGGT CAATGTCAAG GTCAACTACG ACAACGTGGG ACTCGGCTAC 
CTCTCTCTGC TGCAGATTGC CACATTCAAA GGCTGGATGG ATATCATGTA TGCTGCAGTG 
GATTCTAGAG AGGTGGATGA GCAGCCATCA TATGAAATCA ACCTTTACAT GTACCTTTAT 
TTTGTTATTT TCATCATTTT TGGCTCCTTT TTTACTCTCA ACCTCTTTAT TGGTGTCATC 
ATTGACAACT TCAATCAGCA AAAATCAAAG TTTGGAGGGA AAGACATTTT CATGACTGAG 
GAACAGAAAA AGTATTACAA TGCCATGAAG AAGCTGGGTG CAAAGAAACG TCCAAAACCT 
ATACCTCGAC CATCAAATAT TATCCAGGGT TTGGTGTTTG ACTTCATATC AAAACAGTTC 
TTTGACATTT TTATCATGGT GCTAATCTGC CTCAACATGG TGACCATGAT GATAGAGACG 
GATGACCAGA GTGCTGAGAA AGAATATGTC CTGTACCAGA TCAATCTGGT CTTCATCGTC 
GTCTTCACAA GTGAATGTGT ACTTAAATTA TTTGCACTCA GACAGTACTT TTTCACTATT 
GGCTGGAACG TCTTTGATTT TGTTGTAGTC ATCCTTTCTA TTGCAGGTCT AATGTTATCA 
GACATCATTG AGAAGTACTT TGTGTCTCCT ACTTTGTTTC GTGTCATCAG ACTTGCAAGA 
ATCGGCCGTG TGCTGCGTCT CATCAGAGGG GCGAAGGGCA TTCGGACCCT TTTGTTTGCC 
TTGATGATGT CACTTCCTGC CCTTTTCAAC ATTGGACTGC TTCTGTTCCT GATCATGTTC 
ATCTTCTCCA TCTTTGGCAT GTCTAACTTT GCCTATGTGA AAAAGCAGGC AGGCATCGAT 
GACATCTTCA ACTTTGAAAC ATTTGGTGGC AGCATTATCT GCCTGTTTGA GATCACAACC 
TCGGCAGGCT GGGACGGCCT TCTGCTGCCT ATTCTAAACA GTGGCCCTCC TGATTGTGAC 
CCTGACTTTG AAAACCCTGG TACAGATGTG CGGGGAAACT GCGGGAATCC TGGCATGGGC 
ATCATGTTTT TCTGCAGTTA CATCATCATG TCATTCCTGG TGGTCGTGAA CATGTACATC 
GCCATCATTC TGGAGAACTT CAACAATGCT CAAGAGGAAA GTGGAGACCC GCTCTGTGAG 
GATGACTTTG ATATGTTTGA TGAAACATGG GAAAAGTTTG ACGTCGATGC TACGCAGTTT 
ATTGAATACG ACCGCTTGTT TGACTTTGTC GATGCCCTGC AGGAGCCTTT ACGAATTGCC 
AAACCCAACC GTCTCAAGCT GATCTCCATG GACATCCCCA TTGTAAACGG CGACAAAATC 
CACTCTCAGG ATATACTTTT AGCAGTGACC CGGGAAGTAC TTGGCGACAC TATTGAGATG 
GATGCCATGA AGGAGAGCAT CGAAGCCAAG TTCATCATGA ACAACCCCAC ATCTGCCTCG 
TTTGAGCCAA TCATCACCAC TCTCAGGAGG AAGGAGGAAG AGCGAGCAGC CATTGCGGTT 
CAAAGAATAT ACCGTAGGCA TCTTCTCAAG CGTGCCATCC GCTATGCCTG CTTCATGCGC 
CAATCAAAGA GGAAAGTTAG GAATCCCAAC GACAATGAAC CTCCTGAAAC TGAAGGGTTG 
ATTGCAAGGA AGATGAATAC CCTATATGGC AGCAATCCAG AACTTGCCAT GGCTCTTGAA 
TTGGAGACTA GGCCAATGCG GCCAAATTCA CAGCCTCCTA AACCAAGCCA GGTCACACAG 
ACCAGAGCAT CAGTGACCTT CCCTAGGCCG CAAGGACAGC TCATCCTCCC AGTGGAGCTC 
ACCAGTGAGG TGATCCTCCG GTCTGCACCC ACTACACACA GCTTCAACAG TTCAGAGAAT 
GCAACAACGA TAAAGGAGTC AATAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213968.1
CDS	1..5490
Translation	MARLLPPTGTSVFRRFTPESLVEIERLIQEKSTREELEGAEEEPQAPSSDLEAGKCLPMIYGDPPGDLLNTPLEDIDPFYKTQKTFIVISKGNTIFRFSSEPAMFCISPFSIVRRGAIKILIHSLFSMFIMITILSNCVFMTMSNPPAWSKTVEYVFTGIYTFEATVKVLSRGFCIGPFTFLRDPWNWLDFMVISMAYVTEFVDLGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKMIDVMILTIFALAVFALIGLQLFMGNLRQKCIRWPILNSTIFDVYNSNMVNDTTLNVTDTFDFKAYISNEENQYFLEGSLDALLCGNSSDAGRCPEGYTCMKAGRNPNYGYTSYDNFGWAFLALFRLMTQDFWENLFQLTLRAAGKTYMIFFVVVIFLGSFYLINLILAVVAMAYDEQNEATLAEARDKEEEFQRLLEQLKNQETGSKASLASQKTQSRGSNRTGSLHDLADEDVIKDCNGRIVPRLIVNRVSSNKELSAEEDQKSLSSKHSMQYLDQPKLSKRTASALSVLTATMEGLEDAQRPCPPGWYKFADMFLKWDCCAPWILFKKWVHFVVMDPFVDLGITICIVLNTLFMAMEHYPMSPHFEHVLSVGNLVFTGIFTAEMVFKLIAMDPYYYFQVGWNIFDSIIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKISEDCELPRWHMNDFFHSFLIVFRILCGEWIETMWDCMEVAGASMCLIVFMMVMVIGNLVVLNLFLALLLSSFSGDNLSGGDDDGEMNNLQIAIGRITRGIDWVKALVASMVQRILGKKPDNTKEEGEGDIELYALNHLDEGKMADGLTNCLSPTLTVPIARCESDVEEDEDSESSDEEDAKATLNDGDSSVCSTVDYQPPEPEPEPEEVEEEEPEPEEPEACFTEGCIRRCACLSVDITEGWGKKWWNLRRTCFTIVEHDYFETFIIFMILLSSGALAFEDINIERRRVIKTILEYADKVFTYIFIVEMLLKWVAYGFKTYFTNAWCWLDFLIVDVSLVSLTANLMGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIFNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTEERIPMDVVNNKSDCMALMYTNEVRWVNVKVNYDNVGLGYLSLLQIATFKGWMDIMYAAVDSREVDEQPSYEINLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKSKFGGKDIFMTEEQKKYYNAMKKLGAKKRPKPIPRPSNIIQGLVFDFISKQFFDIFIMVLICLNMVTMMIETDDQSAEKEYVLYQINLVFIVVFTSECVLKLFALRQYFFTIGWNVFDFVVVILSIAGLMLSDIIEKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLIMFIFSIFGMSNFAYVKKQAGIDDIFNFETFGGSIICLFEITTSAGWDGLLLPILNSGPPDCDPDFENPGTDVRGNCGNPGMGIMFFCSYIIMSFLVVVNMYIAIILENFNNAQEESGDPLCEDDFDMFDETWEKFDVDATQFIEYDRLFDFVDALQEPLRIAKPNRLKLISMDIPIVNGDKIHSQDILLAVTREVLGDTIEMDAMKESIEAKFIMNNPTSASFEPIITTLRRKEEERAAIAVQRIYRRHLLKRAIRYACFMRQSKRKVRNPNDNEPPETEGLIARKMNTLYGSNPELAMALELETRPMRPNSQPPKPSQVTQTRASVTFPRPQGQLILPVELTSEVILRSAPTTHSFNSSENATTIKESIV

Target ORF information:

RefSeq Version	XM_017358479.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sodium channel, voltage-gated, type IV, alpha, a (scn4aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358479.1

ORF Insert Sequence:

ATGGCACGCC TGCTCCCTCC CACAGGCACC AGTGTGTTCC GCCGCTTCAC CCCAGAGTCA 
CTGGTGGAGA TCGAGAGGCT CATCCAGGAG AAGAGCACCA GGGAGGAACT AGAGGGAGCC 
GAGGAGGAAC CACAGGCACC GAGCAGCGAT CTGGAGGCGG GAAAATGCCT GCCGATGATC 
TATGGCGACC CCCCCGGGGA TCTCCTCAAC ACCCCTCTGG AAGACATCGA CCCCTTTTAT 
AAAACACAGA AAACTTTTAT TGTCATAAGC AAGGGAAACA CAATCTTCAG GTTCTCTTCT 
GAACCGGCGA TGTTTTGCAT CAGTCCTTTT AGTATTGTCA GAAGAGGAGC CATCAAAATT 
CTTATACATT CGTTATTTAG CATGTTCATC ATGATCACCA TCCTGTCCAA TTGCGTGTTC 
ATGACGATGA GTAACCCTCC AGCCTGGAGT AAAACTGTCG AGTATGTTTT TACTGGTATA 
TATACCTTTG AAGCGACTGT CAAAGTTTTA TCCCGAGGCT TCTGCATTGG ACCTTTTACA 
TTCCTCCGTG ATCCATGGAA CTGGCTTGAT TTCATGGTGA TCAGCATGGC GTACGTCACA 
GAGTTTGTAG ACCTTGGCAA TGTGTCAGCA CTGAGGACAT TTCGTGTGCT TCGAGCTCTC 
AAAACAATCA CAGTTATTCC TGGTTTGAAA ACCATTGTTG GGGCTTTGAT TCAATCTGTC 
AAGAAGATGA TAGACGTCAT GATTTTGACC ATCTTTGCAC TGGCTGTATT TGCCCTCATT 
GGACTACAGC TGTTCATGGG AAATCTGCGC CAAAAGTGCA TCCGTTGGCC CATTCTCAAC 
AGCACCATCT TTGACGTCTA CAACTCCAAC ATGGTCAATG ACACCACCCT CAACGTTACC 
GATACTTTCG ATTTCAAAGC TTACATAAGC AATGAAGAAA ACCAATACTT TCTGGAAGGA 
AGTTTAGATG CTTTACTATG TGGCAACAGT TCTGATGCTG GGAGATGTCC AGAAGGATAC 
ACGTGTATGA AAGCTGGTCG AAACCCAAAC TATGGCTATA CAAGCTATGA CAACTTTGGA 
TGGGCCTTTC TCGCCCTCTT CAGACTCATG ACCCAGGACT TTTGGGAGAA TCTTTTCCAG 
CTGACCCTTC GTGCTGCTGG GAAGACTTAC ATGATCTTCT TCGTGGTGGT CATCTTCCTG 
GGCTCTTTCT ACCTCATCAA TCTGATCTTG GCTGTGGTTG CAATGGCATA CGACGAGCAG 
AATGAGGCCA CATTGGCCGA GGCTCGTGAC AAAGAAGAGG AGTTTCAAAG ATTACTTGAG 
CAGCTCAAGA ACCAAGAGAC AGGCAGTAAA GCATCTTTGG CCAGCCAGAA AACACAAAGT 
CGAGGGTCAA ACCGGACAGG TTCCCTGCAC GATCTTGCAG ATGAAGATGT CATAAAGGAC 
TGTAATGGCC GAATAGTACC TCGCCTTATT GTCAACAGAG TTTCCTCTAA CAAAGAGTTA 
TCAGCAGAAG AGGACCAGAA GTCTCTGAGT TCAAAACACA GCATGCAGTA CTTGGACCAG 
CCCAAACTCT CCAAGAGAAC AGCTAGTGCT CTCAGTGTAC TCACTGCAAC TATGGAGGGG 
TTGGAGGATG CCCAGAGGCC TTGTCCCCCT GGCTGGTATA AGTTTGCGGA TATGTTCCTT 
AAATGGGACT GCTGCGCTCC CTGGATCTTG TTTAAGAAGT GGGTTCATTT TGTAGTGATG 
GACCCATTCG TTGACCTGGG TATCACCATC TGCATTGTGC TCAATACACT GTTCATGGCC 
ATGGAGCATT ACCCTATGAG TCCACATTTT GAACATGTCC TCTCAGTGGG CAACTTGGTG 
TTCACAGGAA TCTTCACAGC TGAAATGGTG TTCAAGCTTA TAGCTATGGA CCCTTACTAC 
TACTTCCAGG TGGGCTGGAA CATTTTTGAC AGCATCATTG TCACACTCAG CCTGGTGGAG 
TTGGGACTGG CCAACGTTCA GGGATTGTCC GTTCTAAGGT CCTTTCGTTT GCTACGTGTC 
TTCAAACTGG CTAAATCTTG GCCCACCCTT AACATGCTGA TCAAGATCAT CGGCAACTCA 
GTGGGTGCTC TAGGGAACCT AACACTTGTT CTGGCCATCA TTGTCTTCAT CTTTGCCGTG 
GTGGGCATGC AGCTTTTTGG AAAAAGCTAC AAGGACTGCG TTTGTAAGAT CTCTGAGGAT 
TGCGAGCTGC CCCGCTGGCA CATGAACGAC TTCTTCCACT CATTCCTCAT CGTCTTTCGG 
ATCTTATGTG GAGAGTGGAT TGAGACTATG TGGGACTGCA TGGAGGTGGC AGGAGCTAGC 
ATGTGTTTGA TAGTCTTCAT GATGGTCATG GTCATCGGAA ACCTTGTGGT GCTGAATCTG 
TTTCTGGCCC TGCTGCTTAG CTCCTTCAGT GGAGATAACC TGTCTGGAGG TGATGATGAT 
GGAGAGATGA ACAACCTTCA GATTGCCATT GGCCGCATCA CCAGGGGTAT CGATTGGGTT 
AAAGCCTTAG TTGCCAGTAT GGTGCAACGG ATTCTGGGAA AGAAACCTGA TAATACCAAA 
GAGGAAGGGG AGGGAGACAT AGAACTGTAC GCTTTAAATC ACCTTGATGA AGGAAAAATG 
GCAGATGGTT TGACCAACTG TTTGTCTCCA ACATTGACTG TACCTATTGC TCGTTGTGAG 
TCTGATGTGG AGGAAGATGA GGACTCTGAA TCTTCTGATG AGGAAGACGC AAAGGCCACT 
CTTAATGATG GTGACTCTTC GGTTTGTAGC ACAGTGGACT ACCAGCCTCC AGAACCTGAG 
CCTGAACCTG AAGAGGTAGA AGAAGAGGAA CCAGAACCTG AAGAACCAGA GGCCTGTTTC 
ACAGAGGGCT GTATTAGGCG ATGTGCATGT TTGAGTGTTG ACATCACAGA AGGATGGGGT 
AAAAAATGGT GGAACCTCAG AAGGACATGC TTCACCATCG TTGAGCATGA TTACTTTGAG 
ACCTTCATCA TCTTTATGAT CCTCCTTAGC AGTGGAGCAC TGGCTTTTGA GGATATCAAC 
ATTGAGAGGC GCAGAGTGAT CAAGACCATT CTGGAGTATG CTGATAAAGT CTTTACATAT 
ATTTTTATAG TGGAGATGTT ACTGAAGTGG GTGGCATATG GCTTCAAGAC CTACTTCACT 
AATGCATGGT GCTGGCTGGA CTTCCTCATT GTGGATGTGT CTCTGGTCAG TTTAACGGCT 
AATTTAATGG GCTACTCTGA GCTGGGGGCA ATCAAATCTC TCAGGACACT TAGAGCTCTT 
CGTCCACTTC GAGCCCTATC CAGATTTGAA GGAATGAGGG TGGTAGTGAA TGCACTTGTA 
GGTGCCATTC CCTCTATTTT TAACGTGCTC CTGGTGTGTC TGATATTCTG GCTCATCTTC 
AGCATTATGG GGGTCAATCT GTTTGCCGGA AAATTCTACC ACTGCATCAA CACCACCACA 
GAGGAACGGA TCCCCATGGA TGTAGTCAAC AACAAGAGTG ACTGCATGGC ACTGATGTAC 
ACCAACGAGG TGCGATGGGT CAATGTCAAG GTCAACTACG ACAACGTGGG ACTCGGCTAC 
CTCTCTCTGC TGCAGATTGC CACATTCAAA GGCTGGATGG ATATCATGTA TGCTGCAGTG 
GATTCTAGAG AGGTGGATGA GCAGCCATCA TATGAAATCA ACCTTTACAT GTACCTTTAT 
TTTGTTATTT TCATCATTTT TGGCTCCTTT TTTACTCTCA ACCTCTTTAT TGGTGTCATC 
ATTGACAACT TCAATCAGCA AAAATCAAAG TTTGGAGGGA AAGACATTTT CATGACTGAG 
GAACAGAAAA AGTATTACAA TGCCATGAAG AAGCTGGGTG CAAAGAAACG TCCAAAACCT 
ATACCTCGAC CATCAAATAT TATCCAGGGT TTGGTGTTTG ACTTCATATC AAAACAGTTC 
TTTGACATTT TTATCATGGT GCTAATCTGC CTCAACATGG TGACCATGAT GATAGAGACG 
GATGACCAGA GTGCTGAGAA AGAATATGTC CTGTACCAGA TCAATCTGGT CTTCATCGTC 
GTCTTCACAA GTGAATGTGT ACTTAAATTA TTTGCACTCA GACAGTACTT TTTCACTATT 
GGCTGGAACG TCTTTGATTT TGTTGTAGTC ATCCTTTCTA TTGCAGGTCT AATGTTATCA 
GACATCATTG AGAAGTACTT TGTGTCTCCT ACTTTGTTTC GTGTCATCAG ACTTGCAAGA 
ATCGGCCGTG TGCTGCGTCT CATCAGAGGG GCGAAGGGCA TTCGGACCCT TTTGTTTGCC 
TTGATGATGT CACTTCCTGC CCTTTTCAAC ATTGGACTGC TTCTGTTCCT GATCATGTTC 
ATCTTCTCCA TCTTTGGCAT GTCTAACTTT GCCTATGTGA AAAAGCAGGC AGGCATCGAT 
GACATCTTCA ACTTTGAAAC ATTTGGTGGC AGCATTATCT GCCTGTTTGA GATCACAACC 
TCGGCAGGCT GGGACGGCCT TCTGCTGCCT ATTCTAAACA GTGGCCCTCC TGATTGTGAC 
CCTGACTTTG AAAACCCTGG TACAGATGTG CGGGGAAACT GCGGGAATCC TGGCATGGGC 
ATCATGTTTT TCTGCAGTTA CATCATCATG TCATTCCTGG TGGTCGTGAA CATGTACATC 
GCCATCATTC TGGAGAACTT CAACAATGCT CAAGAGGAAA GTGGAGACCC GCTCTGTGAG 
GATGACTTTG ATATGTTTGA TGAAACATGG GAAAAGTTTG ACGTCGATGC TACGCAGTTT 
ATTGAATACG ACCGCTTGTT TGACTTTGTC GATGCCCTGC AGGAGCCTTT ACGAATTGCC 
AAACCCAACC GTCTCAAGCT GATCTCCATG GACATCCCCA TTGTAAACGG CGACAAAATC 
CACTCTCAGG ATATACTTTT AGCAGTGACC CGGGAAGTAC TTGGCGACAC TATTGAGATG 
GATGCCATGA AGGAGAGCAT CGAAGCCAAG TTCATCATGA ACAACCCCAC ATCTGCCTCG 
TTTGAGCCAA TCATCACCAC TCTCAGGAGG AAGGAGGAAG AGCGAGCAGC CATTGCGGTT 
CAAAGAATAT ACCGTAGGCA TCTTCTCAAG CGTGCCATCC GCTATGCCTG CTTCATGCGC 
CAATCAAAGA GGAAAGTTAG GAATCCCAAC GACAATGAAC CTCCTGAAAC TGAAGGGTTG 
ATTGCAAGGA AGATGAATAC CCTATATGGC AGCAATCCAG AACTTGCCAT GGCTCTTGAA 
TTGGAGACTA GGCCAATGCG GCCAAATTCA CAGCCTCCTA AACCAAGCCA GGTCACACAG 
ACCAGAGCAT CAGTGACCTT CCCTAGGCCG CAAGGACAGC TCATCCTCCC AGTGGAGCTC 
ACCAGTGAGG TGATCCTCCG GTCTGCACCC ACTACACACA GCTTCAACAG TTCAGAGAAT 
GCAACAACGA TAAAGGAGTC AATAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn4aa ( XM_017358479.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213968.1 Danio rerio sodium channel, voltage-gated, type IV, alpha, a (scn4aa), transcript variant X1, mRNA.

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CloneID	ODa46304
Clone ID Related Accession (Same CDS sequence)	XM_017358479.1 , XM_017358479.2
Accession Version	XM_017358479.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	sodium channel protein type 4 subunit alpha A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358479.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACGCC TGCTCCCTCC CACAGGCACC AGTGTGTTCC GCCGCTTCAC CCCAGAGTCA 
CTGGTGGAGA TCGAGAGGCT CATCCAGGAG AAGAGCACCA GGGAGGAACT AGAGGGAGCC 
GAGGAGGAAC CACAGGCACC GAGCAGCGAT CTGGAGGCGG GAAAATGCCT GCCGATGATC 
TATGGCGACC CCCCCGGGGA TCTCCTCAAC ACCCCTCTGG AAGACATCGA CCCCTTTTAT 
AAAACACAGA AAACTTTTAT TGTCATAAGC AAGGGAAACA CAATCTTCAG GTTCTCTTCT 
GAACCGGCGA TGTTTTGCAT CAGTCCTTTT AGTATTGTCA GAAGAGGAGC CATCAAAATT 
CTTATACATT CGTTATTTAG CATGTTCATC ATGATCACCA TCCTGTCCAA TTGCGTGTTC 
ATGACGATGA GTAACCCTCC AGCCTGGAGT AAAACTGTCG AGTATGTTTT TACTGGTATA 
TATACCTTTG AAGCGACTGT CAAAGTTTTA TCCCGAGGCT TCTGCATTGG ACCTTTTACA 
TTCCTCCGTG ATCCATGGAA CTGGCTTGAT TTCATGGTGA TCAGCATGGC GTACGTCACA 
GAGTTTGTAG ACCTTGGCAA TGTGTCAGCA CTGAGGACAT TTCGTGTGCT TCGAGCTCTC 
AAAACAATCA CAGTTATTCC TGGTTTGAAA ACCATTGTTG GGGCTTTGAT TCAATCTGTC 
AAGAAGATGA TAGACGTCAT GATTTTGACC ATCTTTGCAC TGGCTGTATT TGCCCTCATT 
GGACTACAGC TGTTCATGGG AAATCTGCGC CAAAAGTGCA TCCGTTGGCC CATTCTCAAC 
AGCACCATCT TTGACGTCTA CAACTCCAAC ATGGTCAATG ACACCACCCT CAACGTTACC 
GATACTTTCG ATTTCAAAGC TTACATAAGC AATGAAGAAA ACCAATACTT TCTGGAAGGA 
AGTTTAGATG CTTTACTATG TGGCAACAGT TCTGATGCTG GGAGATGTCC AGAAGGATAC 
ACGTGTATGA AAGCTGGTCG AAACCCAAAC TATGGCTATA CAAGCTATGA CAACTTTGGA 
TGGGCCTTTC TCGCCCTCTT CAGACTCATG ACCCAGGACT TTTGGGAGAA TCTTTTCCAG 
CTGACCCTTC GTGCTGCTGG GAAGACTTAC ATGATCTTCT TCGTGGTGGT CATCTTCCTG 
GGCTCTTTCT ACCTCATCAA TCTGATCTTG GCTGTGGTTG CAATGGCATA CGACGAGCAG 
AATGAGGCCA CATTGGCCGA GGCTCGTGAC AAAGAAGAGG AGTTTCAAAG ATTACTTGAG 
CAGCTCAAGA ACCAAGAGAC AGGCAGTAAA GCATCTTTGG CCAGCCAGAA AACACAAAGT 
CGAGGGTCAA ACCGGACAGG TTCCCTGCAC GATCTTGCAG ATGAAGATGT CATAAAGGAC 
TGTAATGGCC GAATAGTACC TCGCCTTATT GTCAACAGAG TTTCCTCTAA CAAAGAGTTA 
TCAGCAGAAG AGGACCAGAA GTCTCTGAGT TCAAAACACA GCATGCAGTA CTTGGACCAG 
CCCAAACTCT CCAAGAGAAC AGCTAGTGCT CTCAGTGTAC TCACTGCAAC TATGGAGGGG 
TTGGAGGATG CCCAGAGGCC TTGTCCCCCT GGCTGGTATA AGTTTGCGGA TATGTTCCTT 
AAATGGGACT GCTGCGCTCC CTGGATCTTG TTTAAGAAGT GGGTTCATTT TGTAGTGATG 
GACCCATTCG TTGACCTGGG TATCACCATC TGCATTGTGC TCAATACACT GTTCATGGCC 
ATGGAGCATT ACCCTATGAG TCCACATTTT GAACATGTCC TCTCAGTGGG CAACTTGGTG 
TTCACAGGAA TCTTCACAGC TGAAATGGTG TTCAAGCTTA TAGCTATGGA CCCTTACTAC 
TACTTCCAGG TGGGCTGGAA CATTTTTGAC AGCATCATTG TCACACTCAG CCTGGTGGAG 
TTGGGACTGG CCAACGTTCA GGGATTGTCC GTTCTAAGGT CCTTTCGTTT GCTACGTGTC 
TTCAAACTGG CTAAATCTTG GCCCACCCTT AACATGCTGA TCAAGATCAT CGGCAACTCA 
GTGGGTGCTC TAGGGAACCT AACACTTGTT CTGGCCATCA TTGTCTTCAT CTTTGCCGTG 
GTGGGCATGC AGCTTTTTGG AAAAAGCTAC AAGGACTGCG TTTGTAAGAT CTCTGAGGAT 
TGCGAGCTGC CCCGCTGGCA CATGAACGAC TTCTTCCACT CATTCCTCAT CGTCTTTCGG 
ATCTTATGTG GAGAGTGGAT TGAGACTATG TGGGACTGCA TGGAGGTGGC AGGAGCTAGC 
ATGTGTTTGA TAGTCTTCAT GATGGTCATG GTCATCGGAA ACCTTGTGGT GCTGAATCTG 
TTTCTGGCCC TGCTGCTTAG CTCCTTCAGT GGAGATAACC TGTCTGGAGG TGATGATGAT 
GGAGAGATGA ACAACCTTCA GATTGCCATT GGCCGCATCA CCAGGGGTAT CGATTGGGTT 
AAAGCCTTAG TTGCCAGTAT GGTGCAACGG ATTCTGGGAA AGAAACCTGA TAATACCAAA 
GAGGAAGGGG AGGGAGACAT AGAACTGTAC GCTTTAAATC ACCTTGATGA AGGAAAAATG 
GCAGATGGTT TGACCAACTG TTTGTCTCCA ACATTGACTG TACCTATTGC TCGTTGTGAG 
TCTGATGTGG AGGAAGATGA GGACTCTGAA TCTTCTGATG AGGAAGACGC AAAGGCCACT 
CTTAATGATG GTGACTCTTC GGTTTGTAGC ACAGTGGACT ACCAGCCTCC AGAACCTGAG 
CCTGAACCTG AAGAGGTAGA AGAAGAGGAA CCAGAACCTG AAGAACCAGA GGCCTGTTTC 
ACAGAGGGCT GTATTAGGCG ATGTGCATGT TTGAGTGTTG ACATCACAGA AGGATGGGGT 
AAAAAATGGT GGAACCTCAG AAGGACATGC TTCACCATCG TTGAGCATGA TTACTTTGAG 
ACCTTCATCA TCTTTATGAT CCTCCTTAGC AGTGGAGCAC TGGCTTTTGA GGATATCAAC 
ATTGAGAGGC GCAGAGTGAT CAAGACCATT CTGGAGTATG CTGATAAAGT CTTTACATAT 
ATTTTTATAG TGGAGATGTT ACTGAAGTGG GTGGCATATG GCTTCAAGAC CTACTTCACT 
AATGCATGGT GCTGGCTGGA CTTCCTCATT GTGGATGTGT CTCTGGTCAG TTTAACGGCT 
AATTTAATGG GCTACTCTGA GCTGGGGGCA ATCAAATCTC TCAGGACACT TAGAGCTCTT 
CGTCCACTTC GAGCCCTATC CAGATTTGAA GGAATGAGGG TGGTAGTGAA TGCACTTGTA 
GGTGCCATTC CCTCTATTTT TAACGTGCTC CTGGTGTGTC TGATATTCTG GCTCATCTTC 
AGCATTATGG GGGTCAATCT GTTTGCCGGA AAATTCTACC ACTGCATCAA CACCACCACA 
GAGGAACGGA TCCCCATGGA TGTAGTCAAC AACAAGAGTG ACTGCATGGC ACTGATGTAC 
ACCAACGAGG TGCGATGGGT CAATGTCAAG GTCAACTACG ACAACGTGGG ACTCGGCTAC 
CTCTCTCTGC TGCAGATTGC CACATTCAAA GGCTGGATGG ATATCATGTA TGCTGCAGTG 
GATTCTAGAG AGGTGGATGA GCAGCCATCA TATGAAATCA ACCTTTACAT GTACCTTTAT 
TTTGTTATTT TCATCATTTT TGGCTCCTTT TTTACTCTCA ACCTCTTTAT TGGTGTCATC 
ATTGACAACT TCAATCAGCA AAAATCAAAG TTTGGAGGGA AAGACATTTT CATGACTGAG 
GAACAGAAAA AGTATTACAA TGCCATGAAG AAGCTGGGTG CAAAGAAACG TCCAAAACCT 
ATACCTCGAC CATCAAATAT TATCCAGGGT TTGGTGTTTG ACTTCATATC AAAACAGTTC 
TTTGACATTT TTATCATGGT GCTAATCTGC CTCAACATGG TGACCATGAT GATAGAGACG 
GATGACCAGA GTGCTGAGAA AGAATATGTC CTGTACCAGA TCAATCTGGT CTTCATCGTC 
GTCTTCACAA GTGAATGTGT ACTTAAATTA TTTGCACTCA GACAGTACTT TTTCACTATT 
GGCTGGAACG TCTTTGATTT TGTTGTAGTC ATCCTTTCTA TTGCAGGTCT AATGTTATCA 
GACATCATTG AGAAGTACTT TGTGTCTCCT ACTTTGTTTC GTGTCATCAG ACTTGCAAGA 
ATCGGCCGTG TGCTGCGTCT CATCAGAGGG GCGAAGGGCA TTCGGACCCT TTTGTTTGCC 
TTGATGATGT CACTTCCTGC CCTTTTCAAC ATTGGACTGC TTCTGTTCCT GATCATGTTC 
ATCTTCTCCA TCTTTGGCAT GTCTAACTTT GCCTATGTGA AAAAGCAGGC AGGCATCGAT 
GACATCTTCA ACTTTGAAAC ATTTGGTGGC AGCATTATCT GCCTGTTTGA GATCACAACC 
TCGGCAGGCT GGGACGGCCT TCTGCTGCCT ATTCTAAACA GTGGCCCTCC TGATTGTGAC 
CCTGACTTTG AAAACCCTGG TACAGATGTG CGGGGAAACT GCGGGAATCC TGGCATGGGC 
ATCATGTTTT TCTGCAGTTA CATCATCATG TCATTCCTGG TGGTCGTGAA CATGTACATC 
GCCATCATTC TGGAGAACTT CAACAATGCT CAAGAGGAAA GTGGAGACCC GCTCTGTGAG 
GATGACTTTG ATATGTTTGA TGAAACATGG GAAAAGTTTG ACGTCGATGC TACGCAGTTT 
ATTGAATACG ACCGCTTGTT TGACTTTGTC GATGCCCTGC AGGAGCCTTT ACGAATTGCC 
AAACCCAACC GTCTCAAGCT GATCTCCATG GACATCCCCA TTGTAAACGG CGACAAAATC 
CACTCTCAGG ATATACTTTT AGCAGTGACC CGGGAAGTAC TTGGCGACAC TATTGAGATG 
GATGCCATGA AGGAGAGCAT CGAAGCCAAG TTCATCATGA ACAACCCCAC ATCTGCCTCG 
TTTGAGCCAA TCATCACCAC TCTCAGGAGG AAGGAGGAAG AGCGAGCAGC CATTGCGGTT 
CAAAGAATAT ACCGTAGGCA TCTTCTCAAG CGTGCCATCC GCTATGCCTG CTTCATGCGC 
CAATCAAAGA GGAAAGTTAG GAATCCCAAC GACAATGAAC CTCCTGAAAC TGAAGGGTTG 
ATTGCAAGGA AGATGAATAC CCTATATGGC AGCAATCCAG AACTTGCCAT GGCTCTTGAA 
TTGGAGACTA GGCCAATGCG GCCAAATTCA CAGCCTCCTA AACCAAGCCA GGTCACACAG 
ACCAGAGCAT CAGTGACCTT CCCTAGGCCG CAAGGACAGC TCATCCTCCC AGTGGAGCTC 
ACCAGTGAGG TGATCCTCCG GTCTGCACCC ACTACACACA GCTTCAACAG TTCAGAGAAT 
GCAACAACGA TAAAGGAGTC AATAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213968.1
CDS	187..5676
Translation	MARLLPPTGTSVFRRFTPESLVEIERLIQEKSTREELEGAEEEPQAPSSDLEAGKCLPMIYGDPPGDLLNTPLEDIDPFYKTQKTFIVISKGNTIFRFSSEPAMFCISPFSIVRRGAIKILIHSLFSMFIMITILSNCVFMTMSNPPAWSKTVEYVFTGIYTFEATVKVLSRGFCIGPFTFLRDPWNWLDFMVISMAYVTEFVDLGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKMIDVMILTIFALAVFALIGLQLFMGNLRQKCIRWPILNSTIFDVYNSNMVNDTTLNVTDTFDFKAYISNEENQYFLEGSLDALLCGNSSDAGRCPEGYTCMKAGRNPNYGYTSYDNFGWAFLALFRLMTQDFWENLFQLTLRAAGKTYMIFFVVVIFLGSFYLINLILAVVAMAYDEQNEATLAEARDKEEEFQRLLEQLKNQETGSKASLASQKTQSRGSNRTGSLHDLADEDVIKDCNGRIVPRLIVNRVSSNKELSAEEDQKSLSSKHSMQYLDQPKLSKRTASALSVLTATMEGLEDAQRPCPPGWYKFADMFLKWDCCAPWILFKKWVHFVVMDPFVDLGITICIVLNTLFMAMEHYPMSPHFEHVLSVGNLVFTGIFTAEMVFKLIAMDPYYYFQVGWNIFDSIIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKISEDCELPRWHMNDFFHSFLIVFRILCGEWIETMWDCMEVAGASMCLIVFMMVMVIGNLVVLNLFLALLLSSFSGDNLSGGDDDGEMNNLQIAIGRITRGIDWVKALVASMVQRILGKKPDNTKEEGEGDIELYALNHLDEGKMADGLTNCLSPTLTVPIARCESDVEEDEDSESSDEEDAKATLNDGDSSVCSTVDYQPPEPEPEPEEVEEEEPEPEEPEACFTEGCIRRCACLSVDITEGWGKKWWNLRRTCFTIVEHDYFETFIIFMILLSSGALAFEDINIERRRVIKTILEYADKVFTYIFIVEMLLKWVAYGFKTYFTNAWCWLDFLIVDVSLVSLTANLMGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIFNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTEERIPMDVVNNKSDCMALMYTNEVRWVNVKVNYDNVGLGYLSLLQIATFKGWMDIMYAAVDSREVDEQPSYEINLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKSKFGGKDIFMTEEQKKYYNAMKKLGAKKRPKPIPRPSNIIQGLVFDFISKQFFDIFIMVLICLNMVTMMIETDDQSAEKEYVLYQINLVFIVVFTSECVLKLFALRQYFFTIGWNVFDFVVVILSIAGLMLSDIIEKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLIMFIFSIFGMSNFAYVKKQAGIDDIFNFETFGGSIICLFEITTSAGWDGLLLPILNSGPPDCDPDFENPGTDVRGNCGNPGMGIMFFCSYIIMSFLVVVNMYIAIILENFNNAQEESGDPLCEDDFDMFDETWEKFDVDATQFIEYDRLFDFVDALQEPLRIAKPNRLKLISMDIPIVNGDKIHSQDILLAVTREVLGDTIEMDAMKESIEAKFIMNNPTSASFEPIITTLRRKEEERAAIAVQRIYRRHLLKRAIRYACFMRQSKRKVRNPNDNEPPETEGLIARKMNTLYGSNPELAMALELETRPMRPNSQPPKPSQVTQTRASVTFPRPQGQLILPVELTSEVILRSAPTTHSFNSSENATTIKESIV

Target ORF information:

RefSeq Version	XM_017358479.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sodium channel, voltage-gated, type IV, alpha, a (scn4aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358479.2

ORF Insert Sequence:

ATGGCACGCC TGCTCCCTCC CACAGGCACC AGTGTGTTCC GCCGCTTCAC CCCAGAGTCA 
CTGGTGGAGA TCGAGAGGCT CATCCAGGAG AAGAGCACCA GGGAGGAACT AGAGGGAGCC 
GAGGAGGAAC CACAGGCACC GAGCAGCGAT CTGGAGGCGG GAAAATGCCT GCCGATGATC 
TATGGCGACC CCCCCGGGGA TCTCCTCAAC ACCCCTCTGG AAGACATCGA CCCCTTTTAT 
AAAACACAGA AAACTTTTAT TGTCATAAGC AAGGGAAACA CAATCTTCAG GTTCTCTTCT 
GAACCGGCGA TGTTTTGCAT CAGTCCTTTT AGTATTGTCA GAAGAGGAGC CATCAAAATT 
CTTATACATT CGTTATTTAG CATGTTCATC ATGATCACCA TCCTGTCCAA TTGCGTGTTC 
ATGACGATGA GTAACCCTCC AGCCTGGAGT AAAACTGTCG AGTATGTTTT TACTGGTATA 
TATACCTTTG AAGCGACTGT CAAAGTTTTA TCCCGAGGCT TCTGCATTGG ACCTTTTACA 
TTCCTCCGTG ATCCATGGAA CTGGCTTGAT TTCATGGTGA TCAGCATGGC GTACGTCACA 
GAGTTTGTAG ACCTTGGCAA TGTGTCAGCA CTGAGGACAT TTCGTGTGCT TCGAGCTCTC 
AAAACAATCA CAGTTATTCC TGGTTTGAAA ACCATTGTTG GGGCTTTGAT TCAATCTGTC 
AAGAAGATGA TAGACGTCAT GATTTTGACC ATCTTTGCAC TGGCTGTATT TGCCCTCATT 
GGACTACAGC TGTTCATGGG AAATCTGCGC CAAAAGTGCA TCCGTTGGCC CATTCTCAAC 
AGCACCATCT TTGACGTCTA CAACTCCAAC ATGGTCAATG ACACCACCCT CAACGTTACC 
GATACTTTCG ATTTCAAAGC TTACATAAGC AATGAAGAAA ACCAATACTT TCTGGAAGGA 
AGTTTAGATG CTTTACTATG TGGCAACAGT TCTGATGCTG GGAGATGTCC AGAAGGATAC 
ACGTGTATGA AAGCTGGTCG AAACCCAAAC TATGGCTATA CAAGCTATGA CAACTTTGGA 
TGGGCCTTTC TCGCCCTCTT CAGACTCATG ACCCAGGACT TTTGGGAGAA TCTTTTCCAG 
CTGACCCTTC GTGCTGCTGG GAAGACTTAC ATGATCTTCT TCGTGGTGGT CATCTTCCTG 
GGCTCTTTCT ACCTCATCAA TCTGATCTTG GCTGTGGTTG CAATGGCATA CGACGAGCAG 
AATGAGGCCA CATTGGCCGA GGCTCGTGAC AAAGAAGAGG AGTTTCAAAG ATTACTTGAG 
CAGCTCAAGA ACCAAGAGAC AGGCAGTAAA GCATCTTTGG CCAGCCAGAA AACACAAAGT 
CGAGGGTCAA ACCGGACAGG TTCCCTGCAC GATCTTGCAG ATGAAGATGT CATAAAGGAC 
TGTAATGGCC GAATAGTACC TCGCCTTATT GTCAACAGAG TTTCCTCTAA CAAAGAGTTA 
TCAGCAGAAG AGGACCAGAA GTCTCTGAGT TCAAAACACA GCATGCAGTA CTTGGACCAG 
CCCAAACTCT CCAAGAGAAC AGCTAGTGCT CTCAGTGTAC TCACTGCAAC TATGGAGGGG 
TTGGAGGATG CCCAGAGGCC TTGTCCCCCT GGCTGGTATA AGTTTGCGGA TATGTTCCTT 
AAATGGGACT GCTGCGCTCC CTGGATCTTG TTTAAGAAGT GGGTTCATTT TGTAGTGATG 
GACCCATTCG TTGACCTGGG TATCACCATC TGCATTGTGC TCAATACACT GTTCATGGCC 
ATGGAGCATT ACCCTATGAG TCCACATTTT GAACATGTCC TCTCAGTGGG CAACTTGGTG 
TTCACAGGAA TCTTCACAGC TGAAATGGTG TTCAAGCTTA TAGCTATGGA CCCTTACTAC 
TACTTCCAGG TGGGCTGGAA CATTTTTGAC AGCATCATTG TCACACTCAG CCTGGTGGAG 
TTGGGACTGG CCAACGTTCA GGGATTGTCC GTTCTAAGGT CCTTTCGTTT GCTACGTGTC 
TTCAAACTGG CTAAATCTTG GCCCACCCTT AACATGCTGA TCAAGATCAT CGGCAACTCA 
GTGGGTGCTC TAGGGAACCT AACACTTGTT CTGGCCATCA TTGTCTTCAT CTTTGCCGTG 
GTGGGCATGC AGCTTTTTGG AAAAAGCTAC AAGGACTGCG TTTGTAAGAT CTCTGAGGAT 
TGCGAGCTGC CCCGCTGGCA CATGAACGAC TTCTTCCACT CATTCCTCAT CGTCTTTCGG 
ATCTTATGTG GAGAGTGGAT TGAGACTATG TGGGACTGCA TGGAGGTGGC AGGAGCTAGC 
ATGTGTTTGA TAGTCTTCAT GATGGTCATG GTCATCGGAA ACCTTGTGGT GCTGAATCTG 
TTTCTGGCCC TGCTGCTTAG CTCCTTCAGT GGAGATAACC TGTCTGGAGG TGATGATGAT 
GGAGAGATGA ACAACCTTCA GATTGCCATT GGCCGCATCA CCAGGGGTAT CGATTGGGTT 
AAAGCCTTAG TTGCCAGTAT GGTGCAACGG ATTCTGGGAA AGAAACCTGA TAATACCAAA 
GAGGAAGGGG AGGGAGACAT AGAACTGTAC GCTTTAAATC ACCTTGATGA AGGAAAAATG 
GCAGATGGTT TGACCAACTG TTTGTCTCCA ACATTGACTG TACCTATTGC TCGTTGTGAG 
TCTGATGTGG AGGAAGATGA GGACTCTGAA TCTTCTGATG AGGAAGACGC AAAGGCCACT 
CTTAATGATG GTGACTCTTC GGTTTGTAGC ACAGTGGACT ACCAGCCTCC AGAACCTGAG 
CCTGAACCTG AAGAGGTAGA AGAAGAGGAA CCAGAACCTG AAGAACCAGA GGCCTGTTTC 
ACAGAGGGCT GTATTAGGCG ATGTGCATGT TTGAGTGTTG ACATCACAGA AGGATGGGGT 
AAAAAATGGT GGAACCTCAG AAGGACATGC TTCACCATCG TTGAGCATGA TTACTTTGAG 
ACCTTCATCA TCTTTATGAT CCTCCTTAGC AGTGGAGCAC TGGCTTTTGA GGATATCAAC 
ATTGAGAGGC GCAGAGTGAT CAAGACCATT CTGGAGTATG CTGATAAAGT CTTTACATAT 
ATTTTTATAG TGGAGATGTT ACTGAAGTGG GTGGCATATG GCTTCAAGAC CTACTTCACT 
AATGCATGGT GCTGGCTGGA CTTCCTCATT GTGGATGTGT CTCTGGTCAG TTTAACGGCT 
AATTTAATGG GCTACTCTGA GCTGGGGGCA ATCAAATCTC TCAGGACACT TAGAGCTCTT 
CGTCCACTTC GAGCCCTATC CAGATTTGAA GGAATGAGGG TGGTAGTGAA TGCACTTGTA 
GGTGCCATTC CCTCTATTTT TAACGTGCTC CTGGTGTGTC TGATATTCTG GCTCATCTTC 
AGCATTATGG GGGTCAATCT GTTTGCCGGA AAATTCTACC ACTGCATCAA CACCACCACA 
GAGGAACGGA TCCCCATGGA TGTAGTCAAC AACAAGAGTG ACTGCATGGC ACTGATGTAC 
ACCAACGAGG TGCGATGGGT CAATGTCAAG GTCAACTACG ACAACGTGGG ACTCGGCTAC 
CTCTCTCTGC TGCAGATTGC CACATTCAAA GGCTGGATGG ATATCATGTA TGCTGCAGTG 
GATTCTAGAG AGGTGGATGA GCAGCCATCA TATGAAATCA ACCTTTACAT GTACCTTTAT 
TTTGTTATTT TCATCATTTT TGGCTCCTTT TTTACTCTCA ACCTCTTTAT TGGTGTCATC 
ATTGACAACT TCAATCAGCA AAAATCAAAG TTTGGAGGGA AAGACATTTT CATGACTGAG 
GAACAGAAAA AGTATTACAA TGCCATGAAG AAGCTGGGTG CAAAGAAACG TCCAAAACCT 
ATACCTCGAC CATCAAATAT TATCCAGGGT TTGGTGTTTG ACTTCATATC AAAACAGTTC 
TTTGACATTT TTATCATGGT GCTAATCTGC CTCAACATGG TGACCATGAT GATAGAGACG 
GATGACCAGA GTGCTGAGAA AGAATATGTC CTGTACCAGA TCAATCTGGT CTTCATCGTC 
GTCTTCACAA GTGAATGTGT ACTTAAATTA TTTGCACTCA GACAGTACTT TTTCACTATT 
GGCTGGAACG TCTTTGATTT TGTTGTAGTC ATCCTTTCTA TTGCAGGTCT AATGTTATCA 
GACATCATTG AGAAGTACTT TGTGTCTCCT ACTTTGTTTC GTGTCATCAG ACTTGCAAGA 
ATCGGCCGTG TGCTGCGTCT CATCAGAGGG GCGAAGGGCA TTCGGACCCT TTTGTTTGCC 
TTGATGATGT CACTTCCTGC CCTTTTCAAC ATTGGACTGC TTCTGTTCCT GATCATGTTC 
ATCTTCTCCA TCTTTGGCAT GTCTAACTTT GCCTATGTGA AAAAGCAGGC AGGCATCGAT 
GACATCTTCA ACTTTGAAAC ATTTGGTGGC AGCATTATCT GCCTGTTTGA GATCACAACC 
TCGGCAGGCT GGGACGGCCT TCTGCTGCCT ATTCTAAACA GTGGCCCTCC TGATTGTGAC 
CCTGACTTTG AAAACCCTGG TACAGATGTG CGGGGAAACT GCGGGAATCC TGGCATGGGC 
ATCATGTTTT TCTGCAGTTA CATCATCATG TCATTCCTGG TGGTCGTGAA CATGTACATC 
GCCATCATTC TGGAGAACTT CAACAATGCT CAAGAGGAAA GTGGAGACCC GCTCTGTGAG 
GATGACTTTG ATATGTTTGA TGAAACATGG GAAAAGTTTG ACGTCGATGC TACGCAGTTT 
ATTGAATACG ACCGCTTGTT TGACTTTGTC GATGCCCTGC AGGAGCCTTT ACGAATTGCC 
AAACCCAACC GTCTCAAGCT GATCTCCATG GACATCCCCA TTGTAAACGG CGACAAAATC 
CACTCTCAGG ATATACTTTT AGCAGTGACC CGGGAAGTAC TTGGCGACAC TATTGAGATG 
GATGCCATGA AGGAGAGCAT CGAAGCCAAG TTCATCATGA ACAACCCCAC ATCTGCCTCG 
TTTGAGCCAA TCATCACCAC TCTCAGGAGG AAGGAGGAAG AGCGAGCAGC CATTGCGGTT 
CAAAGAATAT ACCGTAGGCA TCTTCTCAAG CGTGCCATCC GCTATGCCTG CTTCATGCGC 
CAATCAAAGA GGAAAGTTAG GAATCCCAAC GACAATGAAC CTCCTGAAAC TGAAGGGTTG 
ATTGCAAGGA AGATGAATAC CCTATATGGC AGCAATCCAG AACTTGCCAT GGCTCTTGAA 
TTGGAGACTA GGCCAATGCG GCCAAATTCA CAGCCTCCTA AACCAAGCCA GGTCACACAG 
ACCAGAGCAT CAGTGACCTT CCCTAGGCCG CAAGGACAGC TCATCCTCCC AGTGGAGCTC 
ACCAGTGAGG TGATCCTCCG GTCTGCACCC ACTACACACA GCTTCAACAG TTCAGAGAAT 
GCAACAACGA TAAAGGAGTC AATAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn4aa ( XM_017358480.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213969.1 Danio rerio sodium channel, voltage-gated, type IV, alpha, a (scn4aa), transcript variant X2, mRNA.

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CloneID	ODa54469
Clone ID Related Accession (Same CDS sequence)	XM_017358480.1
Accession Version	XM_017358480.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	sodium channel protein type 4 subunit alpha A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACGCC TGCTCCCTCC CACAGGCACC AGTGTGTTCC GCCGCTTCAC CCCAGAGTCA 
CTGGTGGAGA TCGAGAGGCT CATCCAGGAG AAGAGCACCA GGGAGGAACT AGAGGGAGCC 
GAGGAGGAAC CACAGGCACC GAGCAGCGAT CTGGAGGCGG GAAAATGCCT GCCGATGATC 
TATGGCGACC CCCCCGGGGA TCTCCTCAAC ACCCCTCTGG AAGACATCGA CCCCTTTTAT 
AAAACACAGA AAACTTTTAT TGTCATAAGC AAGGGAAACA CAATCTTCAG GTTCTCTTCT 
GAACCGGCGA TGTTTTGCAT CAGTCCTTTT AGTATTGTCA GAAGAGGAGC CATCAAAATT 
CTTATACATT CGTTATTTAG CATGTTCATC ATGATCACCA TCCTGTCCAA TTGCGTGTTC 
ATGACGATGA GTAACCCTCC AGCCTGGAGT AAAACTGTCG AGTATGTTTT TACTGGTATA 
TATACCTTTG AAGCGACTGT CAAAGTTTTA TCCCGAGGCT TCTGCATTGG ACCTTTTACA 
TTCCTCCGTG ATCCATGGAA CTGGCTTGAT TTCATGGTGA TCAGCATGGC GTACGTCACA 
GAGTTTGTAG ACCTTGGCAA TGTGTCAGCA CTGAGGACAT TTCGTGTGCT TCGAGCTCTC 
AAAACAATCA CAGTTATTCC TGGTTTGAAA ACCATTGTTG GGGCTTTGAT TCAATCTGTC 
AAGAAGATGA TAGACGTCAT GATTTTGACC ATCTTTGCAC TGGCTGTATT TGCCCTCATT 
GGACTACAGC TGTTCATGGG AAATCTGCGC CAAAAGTGCA TCCGTTGGCC CATTCTCAAC 
AGCACCATCT TTGACGTCTA CAACTCCAAC ATGGTCAATG ACACCACCCT CAACGTTACC 
GATACTTTCG ATTTCAAAGC TTACATAAGC AATGAAGAAA ACCAATACTT TCTGGAAGGA 
AGTTTAGATG CTTTACTATG TGGCAACAGT TCTGATGCTG GGAGATGTCC AGAAGGATAC 
ACGTGTATGA AAGCTGGTCG AAACCCAAAC TATGGCTATA CAAGCTATGA CAACTTTGGA 
TGGGCCTTTC TCGCCCTCTT CAGACTCATG ACCCAGGACT TTTGGGAGAA TCTTTTCCAG 
CTGACCCTTC GTGCTGCTGG GAAGACTTAC ATGATCTTCT TCGTGGTGGT CATCTTCCTG 
GGCTCTTTCT ACCTCATCAA TCTGATCTTG GCTGTGGTTG CAATGGCATA CGACGAGCAG 
AATGAGGCCA CATTGGCCGA GGCTCGTGAC AAAGAAGAGG AGTTTCAAAG ATTACTTGAG 
CAGCTCAAGA ACCAAGAGCT TAAATTACAG ACAGGCAGTA AAGCATCTTT GGCCAGCCAG 
AAAACACAAA GTCGAGGGTC AAACCGGACA GGTTCCCTGC ACGATCTTGC AGATGAAGAT 
GTCATAAAGG ACTGTAATGG CCGAATAGTA CCTCGCCTTA TTGTCAACAG AGTTTCCTCT 
AACAAAGAGT TATCAGCAGA AGAGGACCAG AAGTCTCTGA GTTCAAAACA CAGCATGCAG 
TACTTGGACC AGCCCAAACT CTCCAAGAGA ACAGCTAGTG CTCTCAGTGT ACTCACTGCA 
ACTATGGAGG GGTTGGAGGA TGCCCAGAGG CCTTGTCCCC CTGGCTGGTA TAAGTTTGCG 
GATATGTTCC TTAAATGGGA CTGCTGCGCT CCCTGGATCT TGTTTAAGAA GTGGGTTCAT 
TTTGTAGTGA TGGACCCATT CGTTGACCTG GGTATCACCA TCTGCATTGT GCTCAATACA 
CTGTTCATGG CCATGGAGCA TTACCCTATG AGTCCACATT TTGAACATGT CCTCTCAGTG 
GGCAACTTGG TGTTCACAGG AATCTTCACA GCTGAAATGG TGTTCAAGCT TATAGCTATG 
GACCCTTACT ACTACTTCCA GGTGGGCTGG AACATTTTTG ACAGCATCAT TGTCACACTC 
AGCCTGGTGG AGTTGGGACT GGCCAACGTT CAGGGATTGT CCGTTCTAAG GTCCTTTCGT 
TTGCTACGTG TCTTCAAACT GGCTAAATCT TGGCCCACCC TTAACATGCT GATCAAGATC 
ATCGGCAACT CAGTGGGTGC TCTAGGGAAC CTAACACTTG TTCTGGCCAT CATTGTCTTC 
ATCTTTGCCG TGGTGGGCAT GCAGCTTTTT GGAAAAAGCT ACAAGGACTG CGTTTGTAAG 
ATCTCTGAGG ATTGCGAGCT GCCCCGCTGG CACATGAACG ACTTCTTCCA CTCATTCCTC 
ATCGTCTTTC GGATCTTATG TGGAGAGTGG ATTGAGACTA TGTGGGACTG CATGGAGGTG 
GCAGGAGCTA GCATGTGTTT GATAGTCTTC ATGATGGTCA TGGTCATCGG AAACCTTGTG 
GTGCTGAATC TGTTTCTGGC CCTGCTGCTT AGCTCCTTCA GTGGAGATAA CCTGTCTGGA 
GGTGATGATG ATGGAGAGAT GAACAACCTT CAGATTGCCA TTGGCCGCAT CACCAGGGGT 
ATCGATTGGG TTAAAGCCTT AGTTGCCAGT ATGGTGCAAC GGATTCTGGG AAAGAAACCT 
GATAATACCA AAGAGGAAGG GGAGGGAGAC ATAGAACTGT ACGCTTTAAA TCACCTTGAT 
GAAGGAAAAA TGGCAGATGG TTTGACCAAC TGTTTGTCTC CAACATTGAC TGTACCTATT 
GCTCGTTGTG AGTCTGATGT GGAGGAAGAT GAGGACTCTG AATCTTCTGA TGAGGAAGAC 
GCAAAGGCCA CTCTTAATGA TGGTGACTCT TCGGTTTGTA GCACAGTGGA CTACCAGCCT 
CCAGAACCTG AGCCTGAACC TGAAGAGGTA GAAGAAGAGG AACCAGAACC TGAAGAACCA 
GAGGCCTGTT TCACAGAGGG CTGTATTAGG CGATGTGCAT GTTTGAGTGT TGACATCACA 
GAAGGATGGG GTAAAAAATG GTGGAACCTC AGAAGGACAT GCTTCACCAT CGTTGAGCAT 
GATTACTTTG AGACCTTCAT CATCTTTATG ATCCTCCTTA GCAGTGGAGC ACTGGCTTTT 
GAGGATATCA ACATTGAGAG GCGCAGAGTG ATCAAGACCA TTCTGGAGTA TGCTGATAAA 
GTCTTTACAT ATATTTTTAT AGTGGAGATG TTACTGAAGT GGGTGGCATA TGGCTTCAAG 
ACCTACTTCA CTAATGCATG GTGCTGGCTG GACTTCCTCA TTGTGGATGT GTCTCTGGTC 
AGTTTAACGG CTAATTTAAT GGGCTACTCT GAGCTGGGGG CAATCAAATC TCTCAGGACA 
CTTAGAGCTC TTCGTCCACT TCGAGCCCTA TCCAGATTTG AAGGAATGAG GGTGGTAGTG 
AATGCACTTG TAGGTGCCAT TCCCTCTATT TTTAACGTGC TCCTGGTGTG TCTGATATTC 
TGGCTCATCT TCAGCATTAT GGGGGTCAAT CTGTTTGCCG GAAAATTCTA CCACTGCATC 
AACACCACCA CAGAGGAACG GATCCCCATG GATGTAGTCA ACAACAAGAG TGACTGCATG 
GCACTGATGT ACACCAACGA GGTGCGATGG GTCAATGTCA AGGTCAACTA CGACAACGTG 
GGACTCGGCT ACCTCTCTCT GCTGCAGATT GCCACATTCA AAGGCTGGAT GGATATCATG 
TATGCTGCAG TGGATTCTAG AGAGGTGGAT GAGCAGCCAT CATATGAAAT CAACCTTTAC 
ATGTACCTTT ATTTTGTTAT TTTCATCATT TTTGGCTCCT TTTTTACTCT CAACCTCTTT 
ATTGGTGTCA TCATTGACAA CTTCAATCAG CAAAAATCAA AGTTTGGAGG GAAAGACATT 
TTCATGACTG AGGAACAGAA AAAGTATTAC AATGCCATGA AGAAGCTGGG TGCAAAGAAA 
CGTCCAAAAC CTATACCTCG ACCATCAAAT ATTATCCAGG GTTTGGTGTT TGACTTCATA 
TCAAAACAGT TCTTTGACAT TTTTATCATG GTGCTAATCT GCCTCAACAT GGTGACCATG 
ATGATAGAGA CGGATGACCA GAGTGCTGAG AAAGAATATG TCCTGTACCA GATCAATCTG 
GTCTTCATCG TCGTCTTCAC AAGTGAATGT GTACTTAAAT TATTTGCACT CAGACAGTAC 
TTTTTCACTA TTGGCTGGAA CGTCTTTGAT TTTGTTGTAG TCATCCTTTC TATTGCAGGT 
CTAATGTTAT CAGACATCAT TGAGAAGTAC TTTGTGTCTC CTACTTTGTT TCGTGTCATC 
AGACTTGCAA GAATCGGCCG TGTGCTGCGT CTCATCAGAG GGGCGAAGGG CATTCGGACC 
CTTTTGTTTG CCTTGATGAT GTCACTTCCT GCCCTTTTCA ACATTGGACT GCTTCTGTTC 
CTGATCATGT TCATCTTCTC CATCTTTGGC ATGTCTAACT TTGCCTATGT GAAAAAGCAG 
GCAGGCATCG ATGACATCTT CAACTTTGAA ACATTTGGTG GCAGCATTAT CTGCCTGTTT 
GAGATCACAA CCTCGGCAGG CTGGGACGGC CTTCTGCTGC CTATTCTAAA CAGTGGCCCT 
CCTGATTGTG ACCCTGACTT TGAAAACCCT GGTACAGATG TGCGGGGAAA CTGCGGGAAT 
CCTGGCATGG GCATCATGTT TTTCTGCAGT TACATCATCA TGTCATTCCT GGTGGTCGTG 
AACATGTACA TCGCCATCAT TCTGGAGAAC TTCAACAATG CTCAAGAGGA AAGTGGAGAC 
CCGCTCTGTG AGGATGACTT TGATATGTTT GATGAAACAT GGGAAAAGTT TGACGTCGAT 
GCTACGCAGT TTATTGAATA CGACCGCTTG TTTGACTTTG TCGATGCCCT GCAGGAGCCT 
TTACGAATTG CCAAACCCAA CCGTCTCAAG CTGATCTCCA TGGACATCCC CATTGTAAAC 
GGCGACAAAA TCCACTCTCA GGATATACTT TTAGCAGTGA CCCGGGAAGT ACTTGGCGAC 
ACTATTGAGA TGGATGCCAT GAAGGAGAGC ATCGAAGCCA AGTTCATCAT GAACAACCCC 
ACATCTGCCT CGTTTGAGCC AATCATCACC ACTCTCAGGA GGAAGGAGGA AGAGCGAGCA 
GCCATTGCGG TTCAAAGAAT ATACCGTAGG CATCTTCTCA AGCGTGCCAT CCGCTATGCC 
TGCTTCATGC GCCAATCAAA GAGGAAAGTT AGGAATCCCA ACGACAATGA ACCTCCTGAA 
ACTGAAGGGT TGATTGCAAG GAAGATGAAT ACCCTATATG GCAGCAATCC AGAACTTGCC 
ATGGCTCTTG AATTGGAGAC TAGGCCAATG CGGCCAAATT CACAGCCTCC TAAACCAAGC 
CAGGTCACAC AGACCAGAGC ATCAGTGACC TTCCCTAGGC CGCAAGGACA GCTCATCCTC 
CCAGTGGAGC TCACCAGTGA GGTGATCCTC CGGTCTGCAC CCACTACACA CAGCTTCAAC 
AGTTCAGAGA ATGCAACAAC GATAAAGGAG TCAATAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213969.1
CDS	1..5502
Translation	MARLLPPTGTSVFRRFTPESLVEIERLIQEKSTREELEGAEEEPQAPSSDLEAGKCLPMIYGDPPGDLLNTPLEDIDPFYKTQKTFIVISKGNTIFRFSSEPAMFCISPFSIVRRGAIKILIHSLFSMFIMITILSNCVFMTMSNPPAWSKTVEYVFTGIYTFEATVKVLSRGFCIGPFTFLRDPWNWLDFMVISMAYVTEFVDLGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKMIDVMILTIFALAVFALIGLQLFMGNLRQKCIRWPILNSTIFDVYNSNMVNDTTLNVTDTFDFKAYISNEENQYFLEGSLDALLCGNSSDAGRCPEGYTCMKAGRNPNYGYTSYDNFGWAFLALFRLMTQDFWENLFQLTLRAAGKTYMIFFVVVIFLGSFYLINLILAVVAMAYDEQNEATLAEARDKEEEFQRLLEQLKNQELKLQTGSKASLASQKTQSRGSNRTGSLHDLADEDVIKDCNGRIVPRLIVNRVSSNKELSAEEDQKSLSSKHSMQYLDQPKLSKRTASALSVLTATMEGLEDAQRPCPPGWYKFADMFLKWDCCAPWILFKKWVHFVVMDPFVDLGITICIVLNTLFMAMEHYPMSPHFEHVLSVGNLVFTGIFTAEMVFKLIAMDPYYYFQVGWNIFDSIIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKISEDCELPRWHMNDFFHSFLIVFRILCGEWIETMWDCMEVAGASMCLIVFMMVMVIGNLVVLNLFLALLLSSFSGDNLSGGDDDGEMNNLQIAIGRITRGIDWVKALVASMVQRILGKKPDNTKEEGEGDIELYALNHLDEGKMADGLTNCLSPTLTVPIARCESDVEEDEDSESSDEEDAKATLNDGDSSVCSTVDYQPPEPEPEPEEVEEEEPEPEEPEACFTEGCIRRCACLSVDITEGWGKKWWNLRRTCFTIVEHDYFETFIIFMILLSSGALAFEDINIERRRVIKTILEYADKVFTYIFIVEMLLKWVAYGFKTYFTNAWCWLDFLIVDVSLVSLTANLMGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIFNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTEERIPMDVVNNKSDCMALMYTNEVRWVNVKVNYDNVGLGYLSLLQIATFKGWMDIMYAAVDSREVDEQPSYEINLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKSKFGGKDIFMTEEQKKYYNAMKKLGAKKRPKPIPRPSNIIQGLVFDFISKQFFDIFIMVLICLNMVTMMIETDDQSAEKEYVLYQINLVFIVVFTSECVLKLFALRQYFFTIGWNVFDFVVVILSIAGLMLSDIIEKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLIMFIFSIFGMSNFAYVKKQAGIDDIFNFETFGGSIICLFEITTSAGWDGLLLPILNSGPPDCDPDFENPGTDVRGNCGNPGMGIMFFCSYIIMSFLVVVNMYIAIILENFNNAQEESGDPLCEDDFDMFDETWEKFDVDATQFIEYDRLFDFVDALQEPLRIAKPNRLKLISMDIPIVNGDKIHSQDILLAVTREVLGDTIEMDAMKESIEAKFIMNNPTSASFEPIITTLRRKEEERAAIAVQRIYRRHLLKRAIRYACFMRQSKRKVRNPNDNEPPETEGLIARKMNTLYGSNPELAMALELETRPMRPNSQPPKPSQVTQTRASVTFPRPQGQLILPVELTSEVILRSAPTTHSFNSSENATTIKESIV

Target ORF information:

RefSeq Version	XM_017358480.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sodium channel, voltage-gated, type IV, alpha, a (scn4aa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358480.1

ORF Insert Sequence:

ATGGCACGCC TGCTCCCTCC CACAGGCACC AGTGTGTTCC GCCGCTTCAC CCCAGAGTCA 
CTGGTGGAGA TCGAGAGGCT CATCCAGGAG AAGAGCACCA GGGAGGAACT AGAGGGAGCC 
GAGGAGGAAC CACAGGCACC GAGCAGCGAT CTGGAGGCGG GAAAATGCCT GCCGATGATC 
TATGGCGACC CCCCCGGGGA TCTCCTCAAC ACCCCTCTGG AAGACATCGA CCCCTTTTAT 
AAAACACAGA AAACTTTTAT TGTCATAAGC AAGGGAAACA CAATCTTCAG GTTCTCTTCT 
GAACCGGCGA TGTTTTGCAT CAGTCCTTTT AGTATTGTCA GAAGAGGAGC CATCAAAATT 
CTTATACATT CGTTATTTAG CATGTTCATC ATGATCACCA TCCTGTCCAA TTGCGTGTTC 
ATGACGATGA GTAACCCTCC AGCCTGGAGT AAAACTGTCG AGTATGTTTT TACTGGTATA 
TATACCTTTG AAGCGACTGT CAAAGTTTTA TCCCGAGGCT TCTGCATTGG ACCTTTTACA 
TTCCTCCGTG ATCCATGGAA CTGGCTTGAT TTCATGGTGA TCAGCATGGC GTACGTCACA 
GAGTTTGTAG ACCTTGGCAA TGTGTCAGCA CTGAGGACAT TTCGTGTGCT TCGAGCTCTC 
AAAACAATCA CAGTTATTCC TGGTTTGAAA ACCATTGTTG GGGCTTTGAT TCAATCTGTC 
AAGAAGATGA TAGACGTCAT GATTTTGACC ATCTTTGCAC TGGCTGTATT TGCCCTCATT 
GGACTACAGC TGTTCATGGG AAATCTGCGC CAAAAGTGCA TCCGTTGGCC CATTCTCAAC 
AGCACCATCT TTGACGTCTA CAACTCCAAC ATGGTCAATG ACACCACCCT CAACGTTACC 
GATACTTTCG ATTTCAAAGC TTACATAAGC AATGAAGAAA ACCAATACTT TCTGGAAGGA 
AGTTTAGATG CTTTACTATG TGGCAACAGT TCTGATGCTG GGAGATGTCC AGAAGGATAC 
ACGTGTATGA AAGCTGGTCG AAACCCAAAC TATGGCTATA CAAGCTATGA CAACTTTGGA 
TGGGCCTTTC TCGCCCTCTT CAGACTCATG ACCCAGGACT TTTGGGAGAA TCTTTTCCAG 
CTGACCCTTC GTGCTGCTGG GAAGACTTAC ATGATCTTCT TCGTGGTGGT CATCTTCCTG 
GGCTCTTTCT ACCTCATCAA TCTGATCTTG GCTGTGGTTG CAATGGCATA CGACGAGCAG 
AATGAGGCCA CATTGGCCGA GGCTCGTGAC AAAGAAGAGG AGTTTCAAAG ATTACTTGAG 
CAGCTCAAGA ACCAAGAGCT TAAATTACAG ACAGGCAGTA AAGCATCTTT GGCCAGCCAG 
AAAACACAAA GTCGAGGGTC AAACCGGACA GGTTCCCTGC ACGATCTTGC AGATGAAGAT 
GTCATAAAGG ACTGTAATGG CCGAATAGTA CCTCGCCTTA TTGTCAACAG AGTTTCCTCT 
AACAAAGAGT TATCAGCAGA AGAGGACCAG AAGTCTCTGA GTTCAAAACA CAGCATGCAG 
TACTTGGACC AGCCCAAACT CTCCAAGAGA ACAGCTAGTG CTCTCAGTGT ACTCACTGCA 
ACTATGGAGG GGTTGGAGGA TGCCCAGAGG CCTTGTCCCC CTGGCTGGTA TAAGTTTGCG 
GATATGTTCC TTAAATGGGA CTGCTGCGCT CCCTGGATCT TGTTTAAGAA GTGGGTTCAT 
TTTGTAGTGA TGGACCCATT CGTTGACCTG GGTATCACCA TCTGCATTGT GCTCAATACA 
CTGTTCATGG CCATGGAGCA TTACCCTATG AGTCCACATT TTGAACATGT CCTCTCAGTG 
GGCAACTTGG TGTTCACAGG AATCTTCACA GCTGAAATGG TGTTCAAGCT TATAGCTATG 
GACCCTTACT ACTACTTCCA GGTGGGCTGG AACATTTTTG ACAGCATCAT TGTCACACTC 
AGCCTGGTGG AGTTGGGACT GGCCAACGTT CAGGGATTGT CCGTTCTAAG GTCCTTTCGT 
TTGCTACGTG TCTTCAAACT GGCTAAATCT TGGCCCACCC TTAACATGCT GATCAAGATC 
ATCGGCAACT CAGTGGGTGC TCTAGGGAAC CTAACACTTG TTCTGGCCAT CATTGTCTTC 
ATCTTTGCCG TGGTGGGCAT GCAGCTTTTT GGAAAAAGCT ACAAGGACTG CGTTTGTAAG 
ATCTCTGAGG ATTGCGAGCT GCCCCGCTGG CACATGAACG ACTTCTTCCA CTCATTCCTC 
ATCGTCTTTC GGATCTTATG TGGAGAGTGG ATTGAGACTA TGTGGGACTG CATGGAGGTG 
GCAGGAGCTA GCATGTGTTT GATAGTCTTC ATGATGGTCA TGGTCATCGG AAACCTTGTG 
GTGCTGAATC TGTTTCTGGC CCTGCTGCTT AGCTCCTTCA GTGGAGATAA CCTGTCTGGA 
GGTGATGATG ATGGAGAGAT GAACAACCTT CAGATTGCCA TTGGCCGCAT CACCAGGGGT 
ATCGATTGGG TTAAAGCCTT AGTTGCCAGT ATGGTGCAAC GGATTCTGGG AAAGAAACCT 
GATAATACCA AAGAGGAAGG GGAGGGAGAC ATAGAACTGT ACGCTTTAAA TCACCTTGAT 
GAAGGAAAAA TGGCAGATGG TTTGACCAAC TGTTTGTCTC CAACATTGAC TGTACCTATT 
GCTCGTTGTG AGTCTGATGT GGAGGAAGAT GAGGACTCTG AATCTTCTGA TGAGGAAGAC 
GCAAAGGCCA CTCTTAATGA TGGTGACTCT TCGGTTTGTA GCACAGTGGA CTACCAGCCT 
CCAGAACCTG AGCCTGAACC TGAAGAGGTA GAAGAAGAGG AACCAGAACC TGAAGAACCA 
GAGGCCTGTT TCACAGAGGG CTGTATTAGG CGATGTGCAT GTTTGAGTGT TGACATCACA 
GAAGGATGGG GTAAAAAATG GTGGAACCTC AGAAGGACAT GCTTCACCAT CGTTGAGCAT 
GATTACTTTG AGACCTTCAT CATCTTTATG ATCCTCCTTA GCAGTGGAGC ACTGGCTTTT 
GAGGATATCA ACATTGAGAG GCGCAGAGTG ATCAAGACCA TTCTGGAGTA TGCTGATAAA 
GTCTTTACAT ATATTTTTAT AGTGGAGATG TTACTGAAGT GGGTGGCATA TGGCTTCAAG 
ACCTACTTCA CTAATGCATG GTGCTGGCTG GACTTCCTCA TTGTGGATGT GTCTCTGGTC 
AGTTTAACGG CTAATTTAAT GGGCTACTCT GAGCTGGGGG CAATCAAATC TCTCAGGACA 
CTTAGAGCTC TTCGTCCACT TCGAGCCCTA TCCAGATTTG AAGGAATGAG GGTGGTAGTG 
AATGCACTTG TAGGTGCCAT TCCCTCTATT TTTAACGTGC TCCTGGTGTG TCTGATATTC 
TGGCTCATCT TCAGCATTAT GGGGGTCAAT CTGTTTGCCG GAAAATTCTA CCACTGCATC 
AACACCACCA CAGAGGAACG GATCCCCATG GATGTAGTCA ACAACAAGAG TGACTGCATG 
GCACTGATGT ACACCAACGA GGTGCGATGG GTCAATGTCA AGGTCAACTA CGACAACGTG 
GGACTCGGCT ACCTCTCTCT GCTGCAGATT GCCACATTCA AAGGCTGGAT GGATATCATG 
TATGCTGCAG TGGATTCTAG AGAGGTGGAT GAGCAGCCAT CATATGAAAT CAACCTTTAC 
ATGTACCTTT ATTTTGTTAT TTTCATCATT TTTGGCTCCT TTTTTACTCT CAACCTCTTT 
ATTGGTGTCA TCATTGACAA CTTCAATCAG CAAAAATCAA AGTTTGGAGG GAAAGACATT 
TTCATGACTG AGGAACAGAA AAAGTATTAC AATGCCATGA AGAAGCTGGG TGCAAAGAAA 
CGTCCAAAAC CTATACCTCG ACCATCAAAT ATTATCCAGG GTTTGGTGTT TGACTTCATA 
TCAAAACAGT TCTTTGACAT TTTTATCATG GTGCTAATCT GCCTCAACAT GGTGACCATG 
ATGATAGAGA CGGATGACCA GAGTGCTGAG AAAGAATATG TCCTGTACCA GATCAATCTG 
GTCTTCATCG TCGTCTTCAC AAGTGAATGT GTACTTAAAT TATTTGCACT CAGACAGTAC 
TTTTTCACTA TTGGCTGGAA CGTCTTTGAT TTTGTTGTAG TCATCCTTTC TATTGCAGGT 
CTAATGTTAT CAGACATCAT TGAGAAGTAC TTTGTGTCTC CTACTTTGTT TCGTGTCATC 
AGACTTGCAA GAATCGGCCG TGTGCTGCGT CTCATCAGAG GGGCGAAGGG CATTCGGACC 
CTTTTGTTTG CCTTGATGAT GTCACTTCCT GCCCTTTTCA ACATTGGACT GCTTCTGTTC 
CTGATCATGT TCATCTTCTC CATCTTTGGC ATGTCTAACT TTGCCTATGT GAAAAAGCAG 
GCAGGCATCG ATGACATCTT CAACTTTGAA ACATTTGGTG GCAGCATTAT CTGCCTGTTT 
GAGATCACAA CCTCGGCAGG CTGGGACGGC CTTCTGCTGC CTATTCTAAA CAGTGGCCCT 
CCTGATTGTG ACCCTGACTT TGAAAACCCT GGTACAGATG TGCGGGGAAA CTGCGGGAAT 
CCTGGCATGG GCATCATGTT TTTCTGCAGT TACATCATCA TGTCATTCCT GGTGGTCGTG 
AACATGTACA TCGCCATCAT TCTGGAGAAC TTCAACAATG CTCAAGAGGA AAGTGGAGAC 
CCGCTCTGTG AGGATGACTT TGATATGTTT GATGAAACAT GGGAAAAGTT TGACGTCGAT 
GCTACGCAGT TTATTGAATA CGACCGCTTG TTTGACTTTG TCGATGCCCT GCAGGAGCCT 
TTACGAATTG CCAAACCCAA CCGTCTCAAG CTGATCTCCA TGGACATCCC CATTGTAAAC 
GGCGACAAAA TCCACTCTCA GGATATACTT TTAGCAGTGA CCCGGGAAGT ACTTGGCGAC 
ACTATTGAGA TGGATGCCAT GAAGGAGAGC ATCGAAGCCA AGTTCATCAT GAACAACCCC 
ACATCTGCCT CGTTTGAGCC AATCATCACC ACTCTCAGGA GGAAGGAGGA AGAGCGAGCA 
GCCATTGCGG TTCAAAGAAT ATACCGTAGG CATCTTCTCA AGCGTGCCAT CCGCTATGCC 
TGCTTCATGC GCCAATCAAA GAGGAAAGTT AGGAATCCCA ACGACAATGA ACCTCCTGAA 
ACTGAAGGGT TGATTGCAAG GAAGATGAAT ACCCTATATG GCAGCAATCC AGAACTTGCC 
ATGGCTCTTG AATTGGAGAC TAGGCCAATG CGGCCAAATT CACAGCCTCC TAAACCAAGC 
CAGGTCACAC AGACCAGAGC ATCAGTGACC TTCCCTAGGC CGCAAGGACA GCTCATCCTC 
CCAGTGGAGC TCACCAGTGA GGTGATCCTC CGGTCTGCAC CCACTACACA CAGCTTCAAC 
AGTTCAGAGA ATGCAACAAC GATAAAGGAG TCAATAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cardiac voltage-gated sodium channel expression and electrophysiological characterization of the sodium current in the zebrafish (Danio rerio) ventricle.
Progress in biophysics and molecular biology13859-68(2018 10)
Haverinen J,Hassinen M,Korajoki H,Vornanen M

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

ZBTB42 mutation defines a novel lethal congenital contracture syndrome (LCCS6).
Human molecular genetics23(24)6584-93(2014 Dec)
Patel N,Smith LL,Faqeih E,Mohamed J,Gupta VA,Alkuraya FS

Drug screening in Scn1a zebrafish mutant identifies clemizole as a potential Dravet syndrome treatment.
Nature communications42410(2013)
Baraban SC,Dinday MT,Hortopan GA

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scn4aa cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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