NUMA1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	NUMA1
Entrez Gene ID	572344
Full Name	nuclear mitotic apparatus protein 1
Synonyms	NuMA,fb44b02,wu:fb44b02
General protein information	Preferred Names nuclear mitotic apparatus protein 1 Names nuclear mitotic apparatus protein 1 nuclear mitotic apparatus protein 1-like
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009291966.3	XP_009290241.1	nuclear mitotic apparatus protein 1 isoform X1
XM_009291967.3	XP_009290242.1	nuclear mitotic apparatus protein 1 isoform X1
NM_001329981.1	NP_001316910.1	nuclear mitotic apparatus protein 1
XM_009291967.2	XP_009290242.1	nuclear mitotic apparatus protein 1 isoform X2
XM_009291966.2	XP_009290241.1	nuclear mitotic apparatus protein 1 isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	numa1	XP_701145.6
Danio rerio (zebrafish)	LOC100332507	XP_002666796.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

The Fz-Dsh planar cell polarity pathway induces oriented cell division via Mud/NuMA in Drosophila and zebrafish.
Ségalen M, Johnston CA, Martin CA, Dumortier JG, Prehoda KE, David NB, Doe CQ, Bellaïche Y
Developmental cell19(5)740-52(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NUMA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUMA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa46285	NM_001329981.1 Latest version!	Danio rerio nuclear mitotic apparatus protein 1 (numa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa46286	XM_009291966.3 Latest version!	Danio rerio nuclear mitotic apparatus protein 1 (numa1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa46286	XM_009291967.3 Latest version!	Danio rerio nuclear mitotic apparatus protein 1 (numa1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
ODa46286	XM_009291967.2	Danio rerio nuclear mitotic apparatus protein 1 (numa1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa46286	XM_009291966.2	Danio rerio nuclear mitotic apparatus protein 1 (numa1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NUMA1 ( NM_001329981.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001316910.1 Danio rerio nuclear mitotic apparatus protein 1 (numa1), mRNA.

Price & Availability↑

CloneID	ODa46285
Clone ID Related Accession (Same CDS sequence)	NM_001329981.1
Accession Version	NM_001329981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1529942400000
Organism	Danio rerio(zebrafish)
Product	nuclear mitotic apparatus protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU655961.14 and FO704909.1. On Aug 12, 2016 this sequence version replaced XM_009291965.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01026284.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAAGTTTA ATAAGAACAA GGCGGGACCA CTTTTGACAT GGATGAACAG CGTGTTTCCA 
GAGAATCAAA TCTGGGAGTT TGGTCATATG AGAGACGGCT TTCGACTCTT GGAGATCTGC 
TTCAAACTAA AGGGGAGGGA GGACGTTGAA GCTTATAAGA CTTTATCCTT ATACAACAAA 
CTGGAAATCA TCTTTAATGA TTTACATGAT GACTTTCACC TGACTGATAG ACAACGTTTC 
CTCATTTTGG GTAAAATCAG TGAAGGACTT GACCTGGAGC TTCAGCTTGC CAAGGTGGCT 
CTTCTCCTTT GCTATTGCAG TTTTAAAAAG ACTAACCCTG TAGCCCTGAA TTCAAGAACA 
GAGTCGGACA TCACTTTCAT GTTTCGTTTT GTAAGAGATG ATGCCAGTGG TTTGTGTTTA 
GACGAAGGCC TCGATCAATT TCTAGACCAA GACTCTGTTA TGAATCTTGC AAACTCCAGT 
AGCGGGAGCA GCTGCAGTTC TCCATTCTAC ATCGATGAGG AGTCTCCGAT GTTTGGTCGT 
TTCCAGCGTC CTCCCAGAGT TCAGTTTCAA GAGCTTTGCA CAGTGGCTTC CAGCTCAGAC 
AGCTCTCCGA TGCAGGATTT GATGAGCACG CCACACTTCC AGTTGAAGAA ACTCCGCAAA 
GAACTGGCGC ATGAAGGAGA CGTGAGAGAT GAGCTGGAGA AAGAGCTGGC CGATCACATC 
AGCATCATCT CAGAGAAAGA GGGTCTGATC TCCCAGTTAC AGCACCGTGT GGAGCGGATG 
CTGCGGGAAC AAGGAGAGCT GGAGAAAGAC CATAAAGCTG CACTGCTGGA GCTCCAGGAG 
AAGAACGAGA GTCTTCTCCA CAGAGTTCAT GAGGTTTTAA AGCAGTGCCA AGATTTAAAA 
ACAGACAACT CTCAAAAAGA GAAAAAGATT GATGAGCTGA CAGAAGAGAA CGGGACTCTC 
AGTGCTCAGC TTCGTAATGT TTTCTCTCAG CTGGCGAGAG CAGAGCAGGA AGTTGCCAAA 
CTCACAGTGG TTCATGAATC TGCACAGACG GAGTGGAAAA ACAGGAAAGA GTTGCTGGAG 
AAGGAGCTGA ATGAGGCCGT CACACACAAG GAGTGTCTGA GTGAGCAGGT GCTGATACTG 
CAGGGGAAGA TTTCTGTTCT GGAGGATGAA CTGAAGAAAG CTCAGTCTCA GGAGAGAGGA 
GAGGTGCTGG GGCCCATCAT GGAGTGGGAA AAGCTCAAAC AAGAGTTGGC TGATCTCACT 
CTTCAACTCG CTCAGCTTCA GGAATCCATT TCCCACCTCG AGAAGAAAAA GGCAGAAGTT 
GAGGCCTTGT TGGCTGAGGA GAGATGCTCT TTTGAAAAAG AAACCGCAAG ACTCCAGTTG 
GTGGTTTTTG ATCTGGAGAA ATCCATCAAC AGCATCCGTC TGGAGAGAGA AACACTTCAG 
GAGGCCTTAA AGACGCAGAA AGAGATGCTC TCTGCGCAGA TATCAGCACT GGAGAGGGAC 
GTTTCCCGTC TGCAACAGGT TGAAGTGCAG CTGACAGCAG AGATCAAAAT ATCTGCAGAT 
CTCCGCCAAC AGAGAGAGGA GCTGGAAGGA AAGGTAGCAT CTTTAGATAG GATGGTTCAT 
TCTCTGCATG CTGAGATCCA GGGGCTGGAA GTGGAGAGAG CTTCTCAACA GGATGCAATT 
AATTCCCTCA TTAGCGATTT ACAAATCGCT AGGGCTACTG TTCAGGAATA TGAGAAGAAG 
CTGGAAGAGC ATCAAAAAGT GGTAGCAGAA AACAATTCCC TGAAAAATGA AGCATGCACA 
ATGCAGCAGG AGCTGAATAA TCATCTAAAG GCAATCGGAG ACCTTCAGGC GGAAGTAAAT 
GAACTCAGAT TTGAGAAAAC TGAGGAAGAG AACAAGGTTA GTGAGGCTTT GGTTAAAATC 
GAAAGCCTCC AGACTGAGAT TCTGCATCTG TGTGAAAAGA TTTCTCTTAA AGATGAAGAA 
ATCAGAAACC TGACGAAAGA GTATGAGTCT GTAGACAACG AGCTGAAGCT TGTGAAGGAG 
CAAAATGTTG AAATAAATGC AATGATCAAA TCTAATCGCA AGGAGCATGA GGAAACTGTT 
GAAAAACTTC AGCAGGAGCT TCATTGCGCT GCTTCTGCCG CATCAGAAAA ACAAGAGCAA 
ATGCTGGTTT TATCAGCAGA GGTAACATCT CTGAAAGAGC AAATCTGCAG ATATAGTGAA 
AATGAGGCAC AGAAACAACA GGAATTGTCT ATTTTAGAAG CACAACACAA TGTGCTGAAG 
GAAAACCTGA CTTCTCTACA GAACCAGTTG GCTGAGGCGA CAACTTCAGC TTCACAGAAG 
GAATCAGAGT TTATTTTGCT TCAACAGGAG CTTTCCCATC AAGAAACACT AAGAGAACAA 
ACTCAGGAGC TTGAGAAGAT TAAACGTGAG GAGCTTGAGG GGATCGTGAG TGAACTTCAG 
GCCAAAATTA TTGAGGTTTC CTCTATTGCC TCAGAAAGGG AAGCTTGTGT GAGCTCCCTT 
CAATATGAGA TGAAAGATCA GCTATCGAAA GCCAAACAGT GTGAAGCTGA TCTACGCAAT 
GAGATGAAGG AAAAGGTTGG AAATTTGCAG GAACTTCTAG ACGCTGCTAA CCATGGAATT 
GCTGAAAAAG AGCATCACTT AGAGTCGTTG AATCAAAGTC TGAAGCAGAT GGAGCTTCTG 
TACTGTCAAA AGGAGGAAGA AATCATTGCA AGTCAACAAG CAAAGCAGCA ACTGGAGAAT 
AATATTGCTG AGCAGAAACA ACAACTAGAT GAGATTCAGC AAAATCGAGA AATACTAAGG 
AATGAAAGAG ACCATCTGTC TACTCGAGTA TCATCTCTCC AAGAGGAAAT TCATCGTTCC 
CAACAGGATT TTTCTGATTT ACAGACTGAC CACAATGTTT TGAAGGAAAA ACTGGTTAAT 
CTTCAGGATC AAGTAGAGCA GGCGATGATT ACTGCTTCCC AGAAGGAATC TGAACTCCTC 
TTGCTTCAAC AGGAGCTTTC CCATCAAGAG ACCCTGAGAG AAAAAGCTTT GGAGCTTGAG 
GCCATCAAGC GAGAAGAGTT TGAGAAAAAA GTGAAGGAAC TGCAGGACAG AATTCTAGAG 
GTGTCAACTC TTGCCTCTCA AAGGGAAGCC CAGGTAATTT CTCTTGAAAA TGAGATGAGG 
GATCAACAGT TGAGTGCCAA CCAATCTAAA GATAATCTCT GCAGGGAGTG GCAGGAGAAG 
CTTGGCATCT TGAAAGGAGA ATTAGATGTA GTCAGTCGAG ACGCTGCAGA CAAAGACCAT 
CAACTGGAAT CTTTGGATCA AAAGCTTAAA GAGATGGAAA TGGTTGTCCT TCAAAAAGAG 
AAAGATGTAA TGGAGACACA TCAGGCAAAG GAGGACCTGG AGAAGAGAAT TGCTGAGCTT 
GAGGAGTGCA AGCAGAAATT AGAGATTATG AGGAATGAAA GAGATCATCT TTCAACTGAA 
GTAGCATCTC TAAAAGAGGA GATTCATAGC TACCAGGATA CTCAAATGCA GAAACAACAA 
ACAATTTCTG TTTTGGAAGT TGAAAACAAT GCTTTAAAGG AAAACATGGC TGCCCTTGAG 
AAACAATTAG CAGAGGAGAT AACTACAGCT TCACAGAAAA ATTCAGAGCT TCAAAACAAG 
CTTCATCAAC AAGAGAGCCT TCACGAAAAG GCTCAAGAGC TTGAGATTGG CAGGCATGAG 
GAATTTGAGA GAAGAGTGAG AGAACTTCAA GCTAAAGTCC AAGAGGTGTC CACTCTTGCT 
TCTGAGAAGG AGGCTGTTGC TTATTCTCTT CAGGAGGAAT TGACGGGTCA TTTGAAGGCT 
AAAGTGTCCG AAGATGATCT CCGTAGAGTC CTGGAGGAAA AAGTGGAAAC CTTACAGAGA 
GAAATAGAAA CTGCTAGTTG TGATGCCACA AGCAAAGACG GTCTTTTGCA GACTTTGGAT 
CAGAAGCTGA GGCAGATGGA GATGCTTTGC CAACAAAAGG AGGATGCAGT TTTTGAGATC 
CAGAACTCAA AAGAAGACTT GCAGAAAGAG ATGAATGAAC TTGTTTCTAA GAATCAAGAA 
CTGGAGGGCT GCCTGCAACA TTTGGAAATG GTAAAAAAGG AAAAAGACCT CCTGTCAAAT 
GAAGTAACTT CTCTGAAAGA GCAAATAAAT GATCAGAGTT TGAGGGCCAA ACAGTCTGAA 
GCTGATCTTT GCATAGTCTT TGAGGAAAAG ATTGAAACCC TTCAAGGACA ACTGGAATCT 
AGTAGTTGTG ACGTCTCGGA CAAAGAGAAA CATCTGCAAA CTTTGCATCA AAAGGTTTCA 
CAGATGGATC TGTTGTGCCA GCAAAAGGAG AATGCAGTTC TTGAGATGCA GAACGCAAAG 
GAAGACTTGC AGAAGAAGAT TGATGAGCTT GTTTCTGAGA AACAACAACT GGAGGGCTGC 
CTGCAAAATT TGGAAATGGT GAAAAGCGAA AGAGACCTCT TGTCAACTGA AGTAACTTCT 
CTAAAAGATC AACTAAATGA TCAGGATTTG AAAGCCAAAC AGTCCGAAGA TGATCTCCGC 
AAAGTGTTGG AAGAAAAGAT TAAAAACCTC CAGGGACAAC TTGAAACTGC TAGTCGTGAT 
GTCTCCGAAA AAGACCAGCT TCTGCAGACT TTGGATCAAA AGGTTACACA GATGGATCAG 
TTGTGTAAGC AAAAGGAGAA ATATGCTCTG GATTTGCTTC AGGTAAAGGA GAATTTGGAG 
AAGAGGATTG GTGAGATTAC TGTTGAGAAA CAGCAATTGG AAGGATGCCA GCAGAATTTG 
GAAACAGTGA GCAAGGACAA AGACAACCTT TTAAATGAAT TAACCTTTCT TAAAGTTGAA 
ATCCAAAGTT ACCAGGAAAA AGAAGTGCAG ATGAAACATG AGCTTTCTGT TTTAGAGAAT 
GAACACAACA TTTTACAGGA GAACCTGGAT ACTCTTCAGA AGCAAGTGGT GGAGTTGACC 
GCTTCAGCTT CTCAGAAAGA GTCTGAGCTT CAAAATGAGG TTTGCAAACA AGAGAAACTT 
CAAGAAAAGG CTCAGAAACT TGAGAAGGAT GCAGGTGATC TTCAGGCTAA AATTCTAGAG 
ATTTCCACTC TTGCTTCTGA GAGAGAGGCA CAAATAAGTT CTCTTAAAGA TGAAATAAAC 
AGTCAGCATT TGAAAGCTAA ACAGTCTGAA GATGATCTCC TCAGAGTGTT TGAGGAGAAA 
ATTGAAAACC TTCAAGGACA GCTTGAAATT GCTCGTCTTG ATGTCTCGGA CAAAGACCAG 
CTTCTGCAAA CTTTAAATCA GAAACTGAAA CAAATGGAAG AACTATGCAA GCAAAAGGAG 
ACATGTGTCC TTGAGATGCA TCAGACAAAG GAGGATCTGG AGAAGAAAAC TGTTGAGCTT 
CAAGCTGACA AAAGTCAACA GAATCAGCAG AATTTGGAGA TGTTGAGGCA AGAAAAGGAC 
CATCTGAAAT CTCTAAACCA GACTATCCGA AGAGAGTGTG AAGCGTCCCG GAAGATTCGA 
GCTGAGCTGG AGTTGAACGT TGAGGAACAA ACTGCTTCCA TCCTTGCACT TAAAAAGGCT 
TCCCAACAGT GGGAGGAGCA GAATCAGGAG CTCCTGGAGC AGCTGAAGGC AAAGACAGAA 
GCTGTAGAGC ACTACAAAGC ACAGGTGGAG AAGGCCATGA ATCACTACAA TGGTAAAAAG 
CAGCTGCTGC TGGAGGCTCA AGAGCTGAAC AAAACTCTTG AGCAGTCATT GGAGGTCAGC 
AAGAGAGAAG CTAAAGCTCT GGAAACTGAA CTAACCCTGG CTCGCATGGA GCTCAACCAA 
GCCAATGATA AAGAGAAGAG CCTTGCTGCT AAAGTGAAGA CTTTGGAGGC CCAAGTTGAT 
TTTGCTGACA GGCAACTGCG AGAGAAGAGA AGGATTGCAG ACGATGGTGA GGATTTGAGA 
CAAAAAGAAA ATCTGTACTT CAGAGTCCCA GAGAAGCAGC AAGACACCAG TGCAGACAGC 
CTGGAGTTTG AGCTAAACGA TTCTCTCAAC ACTACCTGTC GTTCTGCAGT ACCGGGTGAG 
TCCAGCACTC CACTGGTCCG TAGTTCTGAG CGTTTGGCTG CTAAACGCCG CGCATTGGGG 
GCTGAATCTC TCGAGACACT ATATTTCACA CCTATGGGTA AACAAGGCTA CAAACGCAAA 
GTGGATCACA ATGATGGTTT TGAACGCAAA CTGGAGTCCA GCATCACTTC TATTGGTGAT 
CTCGTGGTTG ATTCTGCAAA GAAATTGACT GCTTCAGCTC GCCGGCGACG CACTACACAA 
GTTATCAACA TCACCTTGGC TAAGAAGGCA CAAGGAAGCT CTGAAAGCGA GGAGTCGTTT 
TCTAGCCTGC ACTCAGCCCG TTCTCATCCA AATTTGGCAG TCCACCAATC ACGGCCGATC 
TCCATTGACT TCTCAGAGGA TGGTCCTGCC ACAACGTTAT CAAAAGCAGA CACACTTCAA 
AATCTGCCTG GATATCGCAG AAGTACAGTG CACAGCGTTG GCCCACCAAG AGCCACCAGT 
ACTTTTTGTA TTGGAGCAGA GAATGAACCG GAGCATGCTG CCGATGACTG GATGCGCATC 
GCTGAACTGC AGTCCCGAAA CAAAGCCTGT CTGCCTCATC TGAAGAGCAG CTATCCTCTG 
GAGTCCAGGC CCAGTCTAGG ACCATCATTC ACTGTAACGG ATGAGGACCT GCGCTTGGGC 
GACCCGAGTG AGACGATCCG GCGTGCGTCC ATGATGCCGG GTCAGATCCT GGAGTCGCTG 
AACTCTCGCC GTTTCTCCCT CGCTCCTGAA ACGAGTTCGA GCCAGGCTCC TGCACGCTCT 
CATCCCCAGC GAGCCACCAT GCTGCCTTGT CAGATCCAAT CCAGCACTGC TGCTCACCGG 
GCCTCACAGC TCACAAAAAC AAGCTCACGT TCACACACTT CAGAGAATAA ACGCAGCCCG 
CTGGCTCCCA AACGCCCGGC ATCACAACTG CAAGGACCAG ACACTCCTGA GGCAAAGAAG 
TTGGCAAGTT GTTTCCCACG GCCAATGACG CCCAAAGGCC GGTTTGGAAA CAGCCAAAAC 
AGACCTCCAG TCTCTCCGGC TGAGCGAAGA CAGTCTGTGA TGTTTACTGT GGTAAATACT 
CCAAAGAACA GTGCTCGTGG AGACAGCAGG CTTCAGCGCG GCCTCAACAA GCTGCGTAAC 
AGCACCCGCA AATCTCCTGC CGTGGCCAGT CGGGCTTTGC GCTCGGCCGT GAGCGGGAAA 
TCTCCCCTGG ACGTCACTCT GAGGAAATCT CCCCGAAACA AGTCCCCGAA AACATCCAAT 
GCCAAAAAGA TCAAGTTAAG GATCAAGGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316910.1
CDS	67..7419
Translation	MKFNKNKAGPLLTWMNSVFPENQIWEFGHMRDGFRLLEICFKLKGREDVEAYKTLSLYNKLEIIFNDLHDDFHLTDRQRFLILGKISEGLDLELQLAKVALLLCYCSFKKTNPVALNSRTESDITFMFRFVRDDASGLCLDEGLDQFLDQDSVMNLANSSSGSSCSSPFYIDEESPMFGRFQRPPRVQFQELCTVASSSDSSPMQDLMSTPHFQLKKLRKELAHEGDVRDELEKELADHISIISEKEGLISQLQHRVERMLREQGELEKDHKAALLELQEKNESLLHRVHEVLKQCQDLKTDNSQKEKKIDELTEENGTLSAQLRNVFSQLARAEQEVAKLTVVHESAQTEWKNRKELLEKELNEAVTHKECLSEQVLILQGKISVLEDELKKAQSQERGEVLGPIMEWEKLKQELADLTLQLAQLQESISHLEKKKAEVEALLAEERCSFEKETARLQLVVFDLEKSINSIRLERETLQEALKTQKEMLSAQISALERDVSRLQQVEVQLTAEIKISADLRQQREELEGKVASLDRMVHSLHAEIQGLEVERASQQDAINSLISDLQIARATVQEYEKKLEEHQKVVAENNSLKNEACTMQQELNNHLKAIGDLQAEVNELRFEKTEEENKVSEALVKIESLQTEILHLCEKISLKDEEIRNLTKEYESVDNELKLVKEQNVEINAMIKSNRKEHEETVEKLQQELHCAASAASEKQEQMLVLSAEVTSLKEQICRYSENEAQKQQELSILEAQHNVLKENLTSLQNQLAEATTSASQKESEFILLQQELSHQETLREQTQELEKIKREELEGIVSELQAKIIEVSSIASEREACVSSLQYEMKDQLSKAKQCEADLRNEMKEKVGNLQELLDAANHGIAEKEHHLESLNQSLKQMELLYCQKEEEIIASQQAKQQLENNIAEQKQQLDEIQQNREILRNERDHLSTRVSSLQEEIHRSQQDFSDLQTDHNVLKEKLVNLQDQVEQAMITASQKESELLLLQQELSHQETLREKALELEAIKREEFEKKVKELQDRILEVSTLASQREAQVISLENEMRDQQLSANQSKDNLCREWQEKLGILKGELDVVSRDAADKDHQLESLDQKLKEMEMVVLQKEKDVMETHQAKEDLEKRIAELEECKQKLEIMRNERDHLSTEVASLKEEIHSYQDTQMQKQQTISVLEVENNALKENMAALEKQLAEEITTASQKNSELQNKLHQQESLHEKAQELEIGRHEEFERRVRELQAKVQEVSTLASEKEAVAYSLQEELTGHLKAKVSEDDLRRVLEEKVETLQREIETASCDATSKDGLLQTLDQKLRQMEMLCQQKEDAVFEIQNSKEDLQKEMNELVSKNQELEGCLQHLEMVKKEKDLLSNEVTSLKEQINDQSLRAKQSEADLCIVFEEKIETLQGQLESSSCDVSDKEKHLQTLHQKVSQMDLLCQQKENAVLEMQNAKEDLQKKIDELVSEKQQLEGCLQNLEMVKSERDLLSTEVTSLKDQLNDQDLKAKQSEDDLRKVLEEKIKNLQGQLETASRDVSEKDQLLQTLDQKVTQMDQLCKQKEKYALDLLQVKENLEKRIGEITVEKQQLEGCQQNLETVSKDKDNLLNELTFLKVEIQSYQEKEVQMKHELSVLENEHNILQENLDTLQKQVVELTASASQKESELQNEVCKQEKLQEKAQKLEKDAGDLQAKILEISTLASEREAQISSLKDEINSQHLKAKQSEDDLLRVFEEKIENLQGQLEIARLDVSDKDQLLQTLNQKLKQMEELCKQKETCVLEMHQTKEDLEKKTVELQADKSQQNQQNLEMLRQEKDHLKSLNQTIRRECEASRKIRAELELNVEEQTASILALKKASQQWEEQNQELLEQLKAKTEAVEHYKAQVEKAMNHYNGKKQLLLEAQELNKTLEQSLEVSKREAKALETELTLARMELNQANDKEKSLAAKVKTLEAQVDFADRQLREKRRIADDGEDLRQKENLYFRVPEKQQDTSADSLEFELNDSLNTTCRSAVPGESSTPLVRSSERLAAKRRALGAESLETLYFTPMGKQGYKRKVDHNDGFERKLESSITSIGDLVVDSAKKLTASARRRRTTQVINITLAKKAQGSSESEESFSSLHSARSHPNLAVHQSRPISIDFSEDGPATTLSKADTLQNLPGYRRSTVHSVGPPRATSTFCIGAENEPEHAADDWMRIAELQSRNKACLPHLKSSYPLESRPSLGPSFTVTDEDLRLGDPSETIRRASMMPGQILESLNSRRFSLAPETSSSQAPARSHPQRATMLPCQIQSSTAAHRASQLTKTSSRSHTSENKRSPLAPKRPASQLQGPDTPEAKKLASCFPRPMTPKGRFGNSQNRPPVSPAERRQSVMFTVVNTPKNSARGDSRLQRGLNKLRNSTRKSPAVASRALRSAVSGKSPLDVTLRKSPRNKSPKTSNAKKIKLRIKV

Target ORF information:

RefSeq Version	NM_001329981.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nuclear mitotic apparatus protein 1 (numa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329981.1

ORF Insert Sequence:

ATGAAGTTTA ATAAGAACAA GGCGGGACCA CTTTTGACAT GGATGAACAG CGTGTTTCCA 
GAGAATCAAA TCTGGGAGTT TGGTCATATG AGAGACGGCT TTCGACTCTT GGAGATCTGC 
TTCAAACTAA AGGGGAGGGA GGACGTTGAA GCTTATAAGA CTTTATCCTT ATACAACAAA 
CTGGAAATCA TCTTTAATGA TTTACATGAT GACTTTCACC TGACTGATAG ACAACGTTTC 
CTCATTTTGG GTAAAATCAG TGAAGGACTT GACCTGGAGC TTCAGCTTGC CAAGGTGGCT 
CTTCTCCTTT GCTATTGCAG TTTTAAAAAG ACTAACCCTG TAGCCCTGAA TTCAAGAACA 
GAGTCGGACA TCACTTTCAT GTTTCGTTTT GTAAGAGATG ATGCCAGTGG TTTGTGTTTA 
GACGAAGGCC TCGATCAATT TCTAGACCAA GACTCTGTTA TGAATCTTGC AAACTCCAGT 
AGCGGGAGCA GCTGCAGTTC TCCATTCTAC ATCGATGAGG AGTCTCCGAT GTTTGGTCGT 
TTCCAGCGTC CTCCCAGAGT TCAGTTTCAA GAGCTTTGCA CAGTGGCTTC CAGCTCAGAC 
AGCTCTCCGA TGCAGGATTT GATGAGCACG CCACACTTCC AGTTGAAGAA ACTCCGCAAA 
GAACTGGCGC ATGAAGGAGA CGTGAGAGAT GAGCTGGAGA AAGAGCTGGC CGATCACATC 
AGCATCATCT CAGAGAAAGA GGGTCTGATC TCCCAGTTAC AGCACCGTGT GGAGCGGATG 
CTGCGGGAAC AAGGAGAGCT GGAGAAAGAC CATAAAGCTG CACTGCTGGA GCTCCAGGAG 
AAGAACGAGA GTCTTCTCCA CAGAGTTCAT GAGGTTTTAA AGCAGTGCCA AGATTTAAAA 
ACAGACAACT CTCAAAAAGA GAAAAAGATT GATGAGCTGA CAGAAGAGAA CGGGACTCTC 
AGTGCTCAGC TTCGTAATGT TTTCTCTCAG CTGGCGAGAG CAGAGCAGGA AGTTGCCAAA 
CTCACAGTGG TTCATGAATC TGCACAGACG GAGTGGAAAA ACAGGAAAGA GTTGCTGGAG 
AAGGAGCTGA ATGAGGCCGT CACACACAAG GAGTGTCTGA GTGAGCAGGT GCTGATACTG 
CAGGGGAAGA TTTCTGTTCT GGAGGATGAA CTGAAGAAAG CTCAGTCTCA GGAGAGAGGA 
GAGGTGCTGG GGCCCATCAT GGAGTGGGAA AAGCTCAAAC AAGAGTTGGC TGATCTCACT 
CTTCAACTCG CTCAGCTTCA GGAATCCATT TCCCACCTCG AGAAGAAAAA GGCAGAAGTT 
GAGGCCTTGT TGGCTGAGGA GAGATGCTCT TTTGAAAAAG AAACCGCAAG ACTCCAGTTG 
GTGGTTTTTG ATCTGGAGAA ATCCATCAAC AGCATCCGTC TGGAGAGAGA AACACTTCAG 
GAGGCCTTAA AGACGCAGAA AGAGATGCTC TCTGCGCAGA TATCAGCACT GGAGAGGGAC 
GTTTCCCGTC TGCAACAGGT TGAAGTGCAG CTGACAGCAG AGATCAAAAT ATCTGCAGAT 
CTCCGCCAAC AGAGAGAGGA GCTGGAAGGA AAGGTAGCAT CTTTAGATAG GATGGTTCAT 
TCTCTGCATG CTGAGATCCA GGGGCTGGAA GTGGAGAGAG CTTCTCAACA GGATGCAATT 
AATTCCCTCA TTAGCGATTT ACAAATCGCT AGGGCTACTG TTCAGGAATA TGAGAAGAAG 
CTGGAAGAGC ATCAAAAAGT GGTAGCAGAA AACAATTCCC TGAAAAATGA AGCATGCACA 
ATGCAGCAGG AGCTGAATAA TCATCTAAAG GCAATCGGAG ACCTTCAGGC GGAAGTAAAT 
GAACTCAGAT TTGAGAAAAC TGAGGAAGAG AACAAGGTTA GTGAGGCTTT GGTTAAAATC 
GAAAGCCTCC AGACTGAGAT TCTGCATCTG TGTGAAAAGA TTTCTCTTAA AGATGAAGAA 
ATCAGAAACC TGACGAAAGA GTATGAGTCT GTAGACAACG AGCTGAAGCT TGTGAAGGAG 
CAAAATGTTG AAATAAATGC AATGATCAAA TCTAATCGCA AGGAGCATGA GGAAACTGTT 
GAAAAACTTC AGCAGGAGCT TCATTGCGCT GCTTCTGCCG CATCAGAAAA ACAAGAGCAA 
ATGCTGGTTT TATCAGCAGA GGTAACATCT CTGAAAGAGC AAATCTGCAG ATATAGTGAA 
AATGAGGCAC AGAAACAACA GGAATTGTCT ATTTTAGAAG CACAACACAA TGTGCTGAAG 
GAAAACCTGA CTTCTCTACA GAACCAGTTG GCTGAGGCGA CAACTTCAGC TTCACAGAAG 
GAATCAGAGT TTATTTTGCT TCAACAGGAG CTTTCCCATC AAGAAACACT AAGAGAACAA 
ACTCAGGAGC TTGAGAAGAT TAAACGTGAG GAGCTTGAGG GGATCGTGAG TGAACTTCAG 
GCCAAAATTA TTGAGGTTTC CTCTATTGCC TCAGAAAGGG AAGCTTGTGT GAGCTCCCTT 
CAATATGAGA TGAAAGATCA GCTATCGAAA GCCAAACAGT GTGAAGCTGA TCTACGCAAT 
GAGATGAAGG AAAAGGTTGG AAATTTGCAG GAACTTCTAG ACGCTGCTAA CCATGGAATT 
GCTGAAAAAG AGCATCACTT AGAGTCGTTG AATCAAAGTC TGAAGCAGAT GGAGCTTCTG 
TACTGTCAAA AGGAGGAAGA AATCATTGCA AGTCAACAAG CAAAGCAGCA ACTGGAGAAT 
AATATTGCTG AGCAGAAACA ACAACTAGAT GAGATTCAGC AAAATCGAGA AATACTAAGG 
AATGAAAGAG ACCATCTGTC TACTCGAGTA TCATCTCTCC AAGAGGAAAT TCATCGTTCC 
CAACAGGATT TTTCTGATTT ACAGACTGAC CACAATGTTT TGAAGGAAAA ACTGGTTAAT 
CTTCAGGATC AAGTAGAGCA GGCGATGATT ACTGCTTCCC AGAAGGAATC TGAACTCCTC 
TTGCTTCAAC AGGAGCTTTC CCATCAAGAG ACCCTGAGAG AAAAAGCTTT GGAGCTTGAG 
GCCATCAAGC GAGAAGAGTT TGAGAAAAAA GTGAAGGAAC TGCAGGACAG AATTCTAGAG 
GTGTCAACTC TTGCCTCTCA AAGGGAAGCC CAGGTAATTT CTCTTGAAAA TGAGATGAGG 
GATCAACAGT TGAGTGCCAA CCAATCTAAA GATAATCTCT GCAGGGAGTG GCAGGAGAAG 
CTTGGCATCT TGAAAGGAGA ATTAGATGTA GTCAGTCGAG ACGCTGCAGA CAAAGACCAT 
CAACTGGAAT CTTTGGATCA AAAGCTTAAA GAGATGGAAA TGGTTGTCCT TCAAAAAGAG 
AAAGATGTAA TGGAGACACA TCAGGCAAAG GAGGACCTGG AGAAGAGAAT TGCTGAGCTT 
GAGGAGTGCA AGCAGAAATT AGAGATTATG AGGAATGAAA GAGATCATCT TTCAACTGAA 
GTAGCATCTC TAAAAGAGGA GATTCATAGC TACCAGGATA CTCAAATGCA GAAACAACAA 
ACAATTTCTG TTTTGGAAGT TGAAAACAAT GCTTTAAAGG AAAACATGGC TGCCCTTGAG 
AAACAATTAG CAGAGGAGAT AACTACAGCT TCACAGAAAA ATTCAGAGCT TCAAAACAAG 
CTTCATCAAC AAGAGAGCCT TCACGAAAAG GCTCAAGAGC TTGAGATTGG CAGGCATGAG 
GAATTTGAGA GAAGAGTGAG AGAACTTCAA GCTAAAGTCC AAGAGGTGTC CACTCTTGCT 
TCTGAGAAGG AGGCTGTTGC TTATTCTCTT CAGGAGGAAT TGACGGGTCA TTTGAAGGCT 
AAAGTGTCCG AAGATGATCT CCGTAGAGTC CTGGAGGAAA AAGTGGAAAC CTTACAGAGA 
GAAATAGAAA CTGCTAGTTG TGATGCCACA AGCAAAGACG GTCTTTTGCA GACTTTGGAT 
CAGAAGCTGA GGCAGATGGA GATGCTTTGC CAACAAAAGG AGGATGCAGT TTTTGAGATC 
CAGAACTCAA AAGAAGACTT GCAGAAAGAG ATGAATGAAC TTGTTTCTAA GAATCAAGAA 
CTGGAGGGCT GCCTGCAACA TTTGGAAATG GTAAAAAAGG AAAAAGACCT CCTGTCAAAT 
GAAGTAACTT CTCTGAAAGA GCAAATAAAT GATCAGAGTT TGAGGGCCAA ACAGTCTGAA 
GCTGATCTTT GCATAGTCTT TGAGGAAAAG ATTGAAACCC TTCAAGGACA ACTGGAATCT 
AGTAGTTGTG ACGTCTCGGA CAAAGAGAAA CATCTGCAAA CTTTGCATCA AAAGGTTTCA 
CAGATGGATC TGTTGTGCCA GCAAAAGGAG AATGCAGTTC TTGAGATGCA GAACGCAAAG 
GAAGACTTGC AGAAGAAGAT TGATGAGCTT GTTTCTGAGA AACAACAACT GGAGGGCTGC 
CTGCAAAATT TGGAAATGGT GAAAAGCGAA AGAGACCTCT TGTCAACTGA AGTAACTTCT 
CTAAAAGATC AACTAAATGA TCAGGATTTG AAAGCCAAAC AGTCCGAAGA TGATCTCCGC 
AAAGTGTTGG AAGAAAAGAT TAAAAACCTC CAGGGACAAC TTGAAACTGC TAGTCGTGAT 
GTCTCCGAAA AAGACCAGCT TCTGCAGACT TTGGATCAAA AGGTTACACA GATGGATCAG 
TTGTGTAAGC AAAAGGAGAA ATATGCTCTG GATTTGCTTC AGGTAAAGGA GAATTTGGAG 
AAGAGGATTG GTGAGATTAC TGTTGAGAAA CAGCAATTGG AAGGATGCCA GCAGAATTTG 
GAAACAGTGA GCAAGGACAA AGACAACCTT TTAAATGAAT TAACCTTTCT TAAAGTTGAA 
ATCCAAAGTT ACCAGGAAAA AGAAGTGCAG ATGAAACATG AGCTTTCTGT TTTAGAGAAT 
GAACACAACA TTTTACAGGA GAACCTGGAT ACTCTTCAGA AGCAAGTGGT GGAGTTGACC 
GCTTCAGCTT CTCAGAAAGA GTCTGAGCTT CAAAATGAGG TTTGCAAACA AGAGAAACTT 
CAAGAAAAGG CTCAGAAACT TGAGAAGGAT GCAGGTGATC TTCAGGCTAA AATTCTAGAG 
ATTTCCACTC TTGCTTCTGA GAGAGAGGCA CAAATAAGTT CTCTTAAAGA TGAAATAAAC 
AGTCAGCATT TGAAAGCTAA ACAGTCTGAA GATGATCTCC TCAGAGTGTT TGAGGAGAAA 
ATTGAAAACC TTCAAGGACA GCTTGAAATT GCTCGTCTTG ATGTCTCGGA CAAAGACCAG 
CTTCTGCAAA CTTTAAATCA GAAACTGAAA CAAATGGAAG AACTATGCAA GCAAAAGGAG 
ACATGTGTCC TTGAGATGCA TCAGACAAAG GAGGATCTGG AGAAGAAAAC TGTTGAGCTT 
CAAGCTGACA AAAGTCAACA GAATCAGCAG AATTTGGAGA TGTTGAGGCA AGAAAAGGAC 
CATCTGAAAT CTCTAAACCA GACTATCCGA AGAGAGTGTG AAGCGTCCCG GAAGATTCGA 
GCTGAGCTGG AGTTGAACGT TGAGGAACAA ACTGCTTCCA TCCTTGCACT TAAAAAGGCT 
TCCCAACAGT GGGAGGAGCA GAATCAGGAG CTCCTGGAGC AGCTGAAGGC AAAGACAGAA 
GCTGTAGAGC ACTACAAAGC ACAGGTGGAG AAGGCCATGA ATCACTACAA TGGTAAAAAG 
CAGCTGCTGC TGGAGGCTCA AGAGCTGAAC AAAACTCTTG AGCAGTCATT GGAGGTCAGC 
AAGAGAGAAG CTAAAGCTCT GGAAACTGAA CTAACCCTGG CTCGCATGGA GCTCAACCAA 
GCCAATGATA AAGAGAAGAG CCTTGCTGCT AAAGTGAAGA CTTTGGAGGC CCAAGTTGAT 
TTTGCTGACA GGCAACTGCG AGAGAAGAGA AGGATTGCAG ACGATGGTGA GGATTTGAGA 
CAAAAAGAAA ATCTGTACTT CAGAGTCCCA GAGAAGCAGC AAGACACCAG TGCAGACAGC 
CTGGAGTTTG AGCTAAACGA TTCTCTCAAC ACTACCTGTC GTTCTGCAGT ACCGGGTGAG 
TCCAGCACTC CACTGGTCCG TAGTTCTGAG CGTTTGGCTG CTAAACGCCG CGCATTGGGG 
GCTGAATCTC TCGAGACACT ATATTTCACA CCTATGGGTA AACAAGGCTA CAAACGCAAA 
GTGGATCACA ATGATGGTTT TGAACGCAAA CTGGAGTCCA GCATCACTTC TATTGGTGAT 
CTCGTGGTTG ATTCTGCAAA GAAATTGACT GCTTCAGCTC GCCGGCGACG CACTACACAA 
GTTATCAACA TCACCTTGGC TAAGAAGGCA CAAGGAAGCT CTGAAAGCGA GGAGTCGTTT 
TCTAGCCTGC ACTCAGCCCG TTCTCATCCA AATTTGGCAG TCCACCAATC ACGGCCGATC 
TCCATTGACT TCTCAGAGGA TGGTCCTGCC ACAACGTTAT CAAAAGCAGA CACACTTCAA 
AATCTGCCTG GATATCGCAG AAGTACAGTG CACAGCGTTG GCCCACCAAG AGCCACCAGT 
ACTTTTTGTA TTGGAGCAGA GAATGAACCG GAGCATGCTG CCGATGACTG GATGCGCATC 
GCTGAACTGC AGTCCCGAAA CAAAGCCTGT CTGCCTCATC TGAAGAGCAG CTATCCTCTG 
GAGTCCAGGC CCAGTCTAGG ACCATCATTC ACTGTAACGG ATGAGGACCT GCGCTTGGGC 
GACCCGAGTG AGACGATCCG GCGTGCGTCC ATGATGCCGG GTCAGATCCT GGAGTCGCTG 
AACTCTCGCC GTTTCTCCCT CGCTCCTGAA ACGAGTTCGA GCCAGGCTCC TGCACGCTCT 
CATCCCCAGC GAGCCACCAT GCTGCCTTGT CAGATCCAAT CCAGCACTGC TGCTCACCGG 
GCCTCACAGC TCACAAAAAC AAGCTCACGT TCACACACTT CAGAGAATAA ACGCAGCCCG 
CTGGCTCCCA AACGCCCGGC ATCACAACTG CAAGGACCAG ACACTCCTGA GGCAAAGAAG 
TTGGCAAGTT GTTTCCCACG GCCAATGACG CCCAAAGGCC GGTTTGGAAA CAGCCAAAAC 
AGACCTCCAG TCTCTCCGGC TGAGCGAAGA CAGTCTGTGA TGTTTACTGT GGTAAATACT 
CCAAAGAACA GTGCTCGTGG AGACAGCAGG CTTCAGCGCG GCCTCAACAA GCTGCGTAAC 
AGCACCCGCA AATCTCCTGC CGTGGCCAGT CGGGCTTTGC GCTCGGCCGT GAGCGGGAAA 
TCTCCCCTGG ACGTCACTCT GAGGAAATCT CCCCGAAACA AGTCCCCGAA AACATCCAAT 
GCCAAAAAGA TCAAGTTAAG GATCAAGGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_009291966.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009290241.1 Danio rerio nuclear mitotic apparatus protein 1 (numa1), transcript variant X1, mRNA.

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CloneID	ODa46286
Clone ID Related Accession (Same CDS sequence)	XM_009291966.3 , XM_009291967.3 , XM_009291967.2 , XM_009291966.2
Accession Version	XM_009291966.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291966.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTTA ATAAGAACAA GGCGGGACCA CTTTTGACAT GGATGAACAG CGTGTTTCCA 
GAGAATCAAA TCTGGGAGTT TGGTCATATG AGAGACGGCT TTCGACTCTT GGAGATCTGC 
TTCAAACTAA AGGGGAGGGA GGACGTTGAA GCTTATAAGA CTTTATCCTT ATACAACAAA 
CTGGAAATCA TCTTTAATGA TTTACATGAT GACTTTCACC TGACTGATAG ACAACGTTTC 
CTCATTTTGG GTAAAATCAG TGAAGGACTT GACCTGGAGC TTCAGCTTGC CAAGGTGGCT 
CTTCTCCTTT GCTATTGCAG TTTTAAAAAG ACTAACCCTG TAGCCCTGAA TTCAAGAACA 
GAGTCGGACA TCACTTTCAT GTTTCGTTTT GTAAGAGATG ATGCCAGTGG TTTGTGTTTA 
GACGAAGGCC TCGATCAATT TCTAGACCAA GACTCTGTTA TGAATCTTGC AAACTCCAGT 
AGCGGGAGCA GCTGCAGTTC TCCATTCTAC ATCGATGAGG AGTCTCCGAT GTTTGGTCGT 
TTCCAGCGTC CTCCCAGAGT TCAGTTTCAA GAGCTTTGCA CAGTGGCTTC CAGCTCAGAC 
AGCTCTCCGA TGCAGGATTT GATGAGCACG CCACACTTCC AGTTGAAGAA ACTCCGCAAA 
GAACTGGCGC ATGAAGGAGA CGTGAGAGAT GAGCTGGAGA AAGAGCTGGC CGATCACATC 
AGCATCATCT CAGAGAAAGA GGGTCTGATC TCCCAGTTAC AGCACCGTGT GGAGCGGATG 
CTGCGGGAAC AAGGAGAGCT GGAGAAAGAC CATAAAGCTG CACTGCTGGA GCTCCAGGAG 
AAGAACGAGA GTCTTCTCCA CAGAGTTCAT GAGGTTTTAA AGCAGTGCCA AGATTTAAAA 
ACAGACAACT CTCAAAAAGA GAAAAAGATT GATGAGCTGA CAGAAGAGAA CGGGACTCTC 
AGTGCTCAGC TTCGTAATGT TTTCTCTCAG CTGGCGAGAG CAGAGCAGGA AGTTGCCAAA 
CTCACAGTGG TTCATGAATC TGCACAGACG GAGTGGAAAA ACAGGAAAGA GTTGCTGGAG 
AAGGAGCTGA ATGAGGCCGT CACACACAAG GAGTGTCTGA GTGAGCAGGT GCTGATACTG 
CAGGGGAAGA TTTCTGTTCT GGAGGATGAA CTGAAGAAAG CTCAGTCTCA GGAGAGAGGA 
GAGGTGCTGG GGCCCATCAT GGAGTGGGAA AAGCTCAAAC AAGAGTTGGC TGATCTCACT 
CTTCAACTCG CTCAGCTTCA GGAATCCATT TCCCACCTCG AGAAGAAAAA GGCAGAAGTT 
GAGGCCTTGT TGGCTGAGGA GAGATGCTCT TTTGAAAAAG AAACCGCAAG ACTCCAGTTG 
GTGGTTTTTG ATCTGGAGAA ATCCATCAAC AGCATCCGTC TGGAGAGAGA AACACTTCAG 
GAGGCCTTAA AGACGCAGAA AGAGATGCTC TCTGCGCAGA TATCAGCACT GGAGAGGGAC 
GTTTCCCGTC TGCAACAGGT TGAAGTGCAG CTGACAGCAG AGATCAAAAT ATCTGCAGAT 
CTCCGCCAAC AGAGAGAGGA GCTGGAAGGA AAGGTAGCAT CTTTAGATAG GATGGTTCAT 
TCTCTGCATG CTGAGATCCA GGGGCTGGAA GTGGAGAGAG CTTCTCAACA GGATGCAATT 
AATTCCCTCA TTAGCGATTT ACAAATCGCT AGGGCTACTG TTCAGGAATA TGAGAAGAAG 
CTGGAAGAGC ATCAAAAAGT GGTAGCAGAA AACAATTCCC TGAAAAATGA AGCATGCACA 
ATGCAGCAGG AGCTGAATAA TCATCTAAAG GCAATCGGAG ACCTTCAGGC GGAAGTAAAT 
GAACTCAGAT TTGAGAAAAC TGAGGAAGAG AACAAGGTTA GTGAGGCTTT GGTTAAAATC 
GAAAGCCTCC AGACTGAGAT TCTGCATCTG TGTGAAAAGA TTTCTCTTAA AGATGAAGAA 
ATCAGAAACC TGACGAAAGA GTATGAGTCT GTAGACAACG AGCTGAAGCT TGTGAAGGAG 
CAAAATGTTG AAATAAATGC AATGATCAAA TCTAATCGCA AGGAGCATGA GGAAACTGTT 
GAAAAACTTC AGCAGGAGCT TCATTGCGCT GCTTCTGCCG CATCAGAAAA ACAAGAGCAA 
ATGCTGGTTT TATCAGCAGA GGTAACATCT CTGAAAGAGC AAATCTGCAG ATATAGTGAA 
AATGAGGCAC AGAAACAACA GGAATTGTCT ATTTTAGAAG CACAACACAA TGTGCTGAAG 
GAAAACCTGA CTTCTCTACA GAACCAGTTG GCTGAGGCGA CAACTTCAGC TTCACAGAAG 
GAATCAGAGT TTATTTTGCT TCAACAGGAG CTTTCCCATC AAGAAACACT AAGAGAACAA 
ACTCAGGAGC TTGAGAAGAT TAAACGTGAG GAGCTTGAGG GGATCGTGAG TGAACTTCAG 
GCCAAAATTA TTGAGGTTTC CTCTATTGCC TCAGAAAGGG AAGCTTGTGT GAGCTCCCTT 
CAATATGAGA TGAAAGATCA GCTATCGAAA GCCAAACAGT GTGAAGCTGA TCTACGCAAT 
GAGATGAAGG AAAAGGTTGG AAATTTGCAG GAACTTCTAG ACGCTGCTAA CCATGGAATT 
GCTGAAAAAG AGCATCACTT AGAGTCGTTG AATCAAAGTC TGAAGCAGAT GGAGCTTCTG 
TACTGTCAAA AGGAGGAAGA AATCATTGCA AGTCAACAAG CAAAGCAGCA ACTGGAGAAT 
AATATTGCTG AGCAGAAACA ACAACTAGAT GAGATTCAGC AAAATCGAGA AATACTAAGG 
AATGAAAGAG ACCATCTGTC TACTCGAGTA TCATCTCTCC AAGAGGAAAT TCATCGTTCC 
CAACAGGATT TTTCTGATTT ACAGACTGAC CACAATGTTT TGAAGGAAAA ACTGGTTAAT 
CTTCAGGATC AAGTAGAGCA GGCGATGATT ACTGCTTCCC AGAAGGAATC TGAACTCCTC 
TTGCTTCAAC AGGAGCTTTC CCATCAAGAG ACCCTGAGAG AAAAAGCTTT GGAGCTTGAG 
GCCATCAAGC GAGAAGAGTT TGAGAAAAAA GTGAAGGAAC TGCAGGACAG AATTCTAGAG 
GTGTCAACTC TTGCCTCTCA AAGGGAAGCC CAGGTAATTT CTCTTGAAAA TGAGATGAGG 
GATCAACAGT TGAGTGCCAA CCAATCTAAA GATAATCTCT GCAGGGAGTG GCAGGAGAAG 
CTTGGCATCT TGAAAGGAGA ATTAGATGTA GTCAGTCGAG ACGCTGCAGA CAAAGACCAT 
CAACTGGAAT CTTTGGATCA AAAGCTTAAA GAGATGGAAA TGGTTGTCCT TCAAAAAGAG 
AAAGATGTAA TGGAGACACA TCAGGCAAAG GAGGACCTGG AGAAGAGAAT TGCTGAGCTT 
GAGGAGTGCA AGCAGAAATT AGAGATTATG AGGAATGAAA GAGATCATCT TTCAACTGAA 
GTAGCATCTC TAAAAGAGGA GATTCATAGC TACCAGGATA CTCAAATGCA GAAACAACAA 
ACAATTTCTG TTTTGGAAGT TGAAAACAAT GCTTTAAAGG AAAACATGGC TGCCCTTGAG 
AAACAATTAG CAGAGGAGAT AACTACAGCT TCACAGAAAA ATTCAGAGCT TCAAAACAAG 
CTTCATCAAC AAGAGAGCCT TCACGAAAAG GCTCAAGAGC TTGAGATTGG CAGGCATGAG 
GAATTTGAGA GAAGAGTGAG AGAACTTCAA GCTAAAGTCC AAGAGGTGTC CACTCTTGCT 
TCTGAGAAGG AGGCTGTTGC TTATTCTCTT CAGGAGGAAT TGACGGGTCA TTTGAAGGCT 
AAAGTGTCCG AAGATGATCT CCGTAGAGTC CTGGAGGAAA AAGTGGAAAC CTTACAGAGA 
GAAATAGAAA CTGCTAGTTG TGATGCCACA AGCAAAGACG GTCTTTTGCA GACTTTGGAT 
CAGAAGCTGA GGCAGATGGA GATGCTTTGC CAACAAAAGG AGGATGCAGT TTTTGAGATC 
CAGAACTCAA AAGAAGACTT GCAGAAAGAG ATGAATGAAC TTGTTTCTAA GAATCAAGAA 
CTGGAGGGCT GCCTGCAACA TTTGGAAATG GTAAAAAAGG AAAAAGACCT CCTGTCAAAT 
GAAGTAACTT CTCTGAAAGA GCAAATAAAT GATCAGAGTT TGAGGGCCAA ACAGTCTGAA 
GCTGATCTTT GCATAGTCTT TGAGGAAAAG ATTGAAACCC TTCAAGGACA ACTGGAATCT 
AGTAGTTGTG ACGTCTCGGA CAAAGAGAAA CATCTGCAAA CTTTGCATCA AAAGGTTTCA 
CAGATGGATC TGTTGTGCCA GCAAAAGGAG AATGCAGTTC TTGAGATGCA GAACGCAAAG 
GAAGACTTGC AGAAGAAGAT TGATGAGCTT GTTTCTGAGA AACAACAACT GGAGGGCTGC 
CTGCAAAATT TGGAAATGGT GAAAAGCGAA AGAGACCTCT TGTCAACTGA AGTAACTTCT 
CTAAAAGATC AACTAAATGA TCAGGATTTG AAAGCCAAAC AGTCCGAAGA TGATCTCCGC 
AAAGTGTTGG AAGAAAAGAT TAAAAACCTC CAGGGACAAC TTGAAACTGC TAGTCGTGAT 
GTCTCCGAAA AAGACCAGCT TCTGCAGACT TTGGATCAAA AGGTTACACA GATGGATCAG 
TTGTGTAAGC AAAAGGAGAA ATATGCTCTG GATTTGCTTC AGGTAAAGGA GAATTTGGAG 
AAGAGGATTG GTGAGATTAC TGTTGAGAAA CAGCAATTGG AAGGATGCCA GCAGAATTTG 
GAAACAGTGA GCAAGGACAA AGACAACCTT TTAAATGAAT TAACCTTTCT TAAAGTTGAA 
ATCCAAAGTT ACCAGGAAAA AGAAGTGCAG ATGAAACATG AGCTTTCTGT TTTAGAGAAT 
GAACACAACA TTTTACAGGA GAACCTGGAT ACTCTTCAGA AGCAAGTGGT GGAGTTGACC 
GCTTCAGCTT CTCAGAAAGA GTCTGAGCTT CAAAATGAGG TTTGCAAACA AGAGAAACTT 
CAAGAAAAGG CTCAGAAACT TGAGAAGGAT GCAGGTGATC TTCAGGCTAA AATTCTAGAG 
ATTTCCACTC TTGCTTCTGA GAGAGAGGCA CAAATAAGTT CTCTTAAAGA TGAAATAAAC 
AGTCAGCATT TGAAAGCTAA ACAGTCTGAA GATGATCTCC TCAGAGTGTT TGAGGAGAAA 
ATTGAAAACC TTCAAGGACA GCTTGAAATT GCTCGTCTTG ATGTCTCGGA CAAAGACCAG 
CTTCTGCAAA CTTTAAATCA GAAACTGAAA CAAATGGAAG AACTATGCAA GCAAAAGGAG 
ACATGTGTCC TTGAGATGCA TCAGACAAAG GAGGATCTGG AGAAGAAAAC TGTTGAGCTT 
CAAGCTGACA AAAGTCAACA GAATCAGCAG AATTTGGAGA TGTTGAGGCA AGAAAAGGAC 
CATCTGAAAT CTCTAAACCA GACTATCCGA AGAGAGTGTG AAGCGTCCCG GAAGATTCGA 
GCTGAGCTGG AGTTGAACGT TGAGGAACAA ACTGCTTCCA TCCTTGCACT TAAAAAGGCT 
TCCCAACAGT GGGAGGAGCA GAATCAGGAG CTCCTGGAGC AGCTGAAGGC AAAGACAGAA 
GCTGTAGAGC ACTACAAAGC ACAGGTGGAG AAGGCCATGA ATCACTACAA TGGTAAAAAG 
CAGCTGCTGC TGGAGGCTCA AGAGCTGAAC AAAACTCTTG AGCAGTCATT GGAGGTCAGC 
AAGAGAGAAG CTAAAGCTCT GGAAACTGAA CTAACCCTGG CTCGCATGGA GCTCAACCAA 
GCCAATGATA AAGAGAAGAG CCTTGCTGCT AAAGTGAAGA CTTTGGAGGC CCAAGTTGAT 
TTTGCTGACA GGCAACTGCG AGAGAAGAGA AGGATTGCAG ACGATGGTGA GGATTTGAGA 
CAAAAAGAAA ATCTGTACTT CAGAGTCCCA GAGAAGCAGC AAGACACCAG TGCAGACAGC 
CTGGAGTTTG AGCTAAACGA TTCTCTCAAC ACTACCTGTC GTTCTGCAGT ACCGGGTGAG 
TCCAGCACTC CACTGGTCCG TAGTTCTGAG CGTTTGGCTG CTAAACGCCG CGCATTGGGG 
GCTGAATCTC TCGAGACACT ATATTTCACA CCTATGGGTA AACAAGGCTA CAAACGCAAA 
GTGGATCACA ATGATGGTTT TGAACGCAAA CTGGAGTCCA GCATCACTTC TATTGGTGAT 
CTCGTGGTTG ATTCTGCAAA GAAATTGACT GCTTCAGCTC GCCGGCGACG CACTACACAA 
GTTATCAACA TCACCTTGGC TAAGAAGGCA CAAGGAAGCT CTGAAAGCGA GGAGTCGTTT 
TCTAGCCTGC ACTCAGCCCG TTCTCATCCA AATTTGGCAG TCCACCAATC ACGGCCGATC 
TCCATTGACT TCTCAGAGGA TGGTCCTGCC ACAACGTTAT CAAAAGCAGA CACACTTCAA 
AATCTGCCTG GATATCGCAG AAGTACAGTG CACAGCGTTG GCCCACCAAG AGCCACCAGT 
ACTTTTTGTA TTGGAGCAGA GAATGAACCG GAGCATGCTG CCGATGACTG GATGCGCATC 
GCTGAACTGC AGTCCCGAAA CAAAGCCTGT CTGCCTCATC TGAAGAGCAG CTATCCTCTG 
GAGTCCAGGC CCAGTCTAGG ACCATCATTC ACTGTAACGG ATGAGGACCT GCGCTTGGGC 
GACCCGAGTG AGACGATCCG GCGTGCGTCC ATGATGCCGG GTCAGATCCT GGAGTCGCTG 
AACTCTCGCC GTTTCTCCCT CGCTCCTGAA ACGAGTTCGA GCCAGGCTCC TGCACGCTCT 
CATCCCCAGC GAGCCACCAT GCTGCCTTGT CAGATCCAAT CCAGCACTGC TGCTCACCGG 
GCCTCACAGC TCACAAAAAC AAGCTCACGT TCACACACTT CAGAGAATAA ACGCAGCCCG 
CTGGCTCCCA AACGCCCGGC ATCACAACTG CAAGGACCAG ACACTCCTGA GGCAAAGAAG 
TTGGCAAGTT GTTTCCCACG GCCAATGACG CCCAAAGGCC GGTTTGGAAA CAGCCAAAAC 
AGACCTCCAG TCTCTCCGGC TGAGCGAAGA CAGTCTGTGA TGTTTACTGT GGTAAATACT 
CCAAAGAACA GTGCTCGTGG AGACAGCAGG CTTCAGCGCG GCCTCAACAA GCTGCGTAAC 
AGCACCCGCA AATCTCCTGC CGTGGCCAGT CGGGCTTTGC GCTCGGCCGT GAGCGGGAAA 
TCTCCCCTGG ACGTCACTCT GAGGAAATCT CCCCGAAACA AGTCCCCGAA AACATCCAAT 
GCCAAAAAGG AAACACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290241.1
CDS	75..7415
Translation	MKFNKNKAGPLLTWMNSVFPENQIWEFGHMRDGFRLLEICFKLKGREDVEAYKTLSLYNKLEIIFNDLHDDFHLTDRQRFLILGKISEGLDLELQLAKVALLLCYCSFKKTNPVALNSRTESDITFMFRFVRDDASGLCLDEGLDQFLDQDSVMNLANSSSGSSCSSPFYIDEESPMFGRFQRPPRVQFQELCTVASSSDSSPMQDLMSTPHFQLKKLRKELAHEGDVRDELEKELADHISIISEKEGLISQLQHRVERMLREQGELEKDHKAALLELQEKNESLLHRVHEVLKQCQDLKTDNSQKEKKIDELTEENGTLSAQLRNVFSQLARAEQEVAKLTVVHESAQTEWKNRKELLEKELNEAVTHKECLSEQVLILQGKISVLEDELKKAQSQERGEVLGPIMEWEKLKQELADLTLQLAQLQESISHLEKKKAEVEALLAEERCSFEKETARLQLVVFDLEKSINSIRLERETLQEALKTQKEMLSAQISALERDVSRLQQVEVQLTAEIKISADLRQQREELEGKVASLDRMVHSLHAEIQGLEVERASQQDAINSLISDLQIARATVQEYEKKLEEHQKVVAENNSLKNEACTMQQELNNHLKAIGDLQAEVNELRFEKTEEENKVSEALVKIESLQTEILHLCEKISLKDEEIRNLTKEYESVDNELKLVKEQNVEINAMIKSNRKEHEETVEKLQQELHCAASAASEKQEQMLVLSAEVTSLKEQICRYSENEAQKQQELSILEAQHNVLKENLTSLQNQLAEATTSASQKESEFILLQQELSHQETLREQTQELEKIKREELEGIVSELQAKIIEVSSIASEREACVSSLQYEMKDQLSKAKQCEADLRNEMKEKVGNLQELLDAANHGIAEKEHHLESLNQSLKQMELLYCQKEEEIIASQQAKQQLENNIAEQKQQLDEIQQNREILRNERDHLSTRVSSLQEEIHRSQQDFSDLQTDHNVLKEKLVNLQDQVEQAMITASQKESELLLLQQELSHQETLREKALELEAIKREEFEKKVKELQDRILEVSTLASQREAQVISLENEMRDQQLSANQSKDNLCREWQEKLGILKGELDVVSRDAADKDHQLESLDQKLKEMEMVVLQKEKDVMETHQAKEDLEKRIAELEECKQKLEIMRNERDHLSTEVASLKEEIHSYQDTQMQKQQTISVLEVENNALKENMAALEKQLAEEITTASQKNSELQNKLHQQESLHEKAQELEIGRHEEFERRVRELQAKVQEVSTLASEKEAVAYSLQEELTGHLKAKVSEDDLRRVLEEKVETLQREIETASCDATSKDGLLQTLDQKLRQMEMLCQQKEDAVFEIQNSKEDLQKEMNELVSKNQELEGCLQHLEMVKKEKDLLSNEVTSLKEQINDQSLRAKQSEADLCIVFEEKIETLQGQLESSSCDVSDKEKHLQTLHQKVSQMDLLCQQKENAVLEMQNAKEDLQKKIDELVSEKQQLEGCLQNLEMVKSERDLLSTEVTSLKDQLNDQDLKAKQSEDDLRKVLEEKIKNLQGQLETASRDVSEKDQLLQTLDQKVTQMDQLCKQKEKYALDLLQVKENLEKRIGEITVEKQQLEGCQQNLETVSKDKDNLLNELTFLKVEIQSYQEKEVQMKHELSVLENEHNILQENLDTLQKQVVELTASASQKESELQNEVCKQEKLQEKAQKLEKDAGDLQAKILEISTLASEREAQISSLKDEINSQHLKAKQSEDDLLRVFEEKIENLQGQLEIARLDVSDKDQLLQTLNQKLKQMEELCKQKETCVLEMHQTKEDLEKKTVELQADKSQQNQQNLEMLRQEKDHLKSLNQTIRRECEASRKIRAELELNVEEQTASILALKKASQQWEEQNQELLEQLKAKTEAVEHYKAQVEKAMNHYNGKKQLLLEAQELNKTLEQSLEVSKREAKALETELTLARMELNQANDKEKSLAAKVKTLEAQVDFADRQLREKRRIADDGEDLRQKENLYFRVPEKQQDTSADSLEFELNDSLNTTCRSAVPGESSTPLVRSSERLAAKRRALGAESLETLYFTPMGKQGYKRKVDHNDGFERKLESSITSIGDLVVDSAKKLTASARRRRTTQVINITLAKKAQGSSESEESFSSLHSARSHPNLAVHQSRPISIDFSEDGPATTLSKADTLQNLPGYRRSTVHSVGPPRATSTFCIGAENEPEHAADDWMRIAELQSRNKACLPHLKSSYPLESRPSLGPSFTVTDEDLRLGDPSETIRRASMMPGQILESLNSRRFSLAPETSSSQAPARSHPQRATMLPCQIQSSTAAHRASQLTKTSSRSHTSENKRSPLAPKRPASQLQGPDTPEAKKLASCFPRPMTPKGRFGNSQNRPPVSPAERRQSVMFTVVNTPKNSARGDSRLQRGLNKLRNSTRKSPAVASRALRSAVSGKSPLDVTLRKSPRNKSPKTSNAKKETQ

Target ORF information:

RefSeq Version	XM_009291966.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nuclear mitotic apparatus protein 1 (numa1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291966.3

ORF Insert Sequence:

ATGAAGTTTA ATAAGAACAA GGCGGGACCA CTTTTGACAT GGATGAACAG CGTGTTTCCA 
GAGAATCAAA TCTGGGAGTT TGGTCATATG AGAGACGGCT TTCGACTCTT GGAGATCTGC 
TTCAAACTAA AGGGGAGGGA GGACGTTGAA GCTTATAAGA CTTTATCCTT ATACAACAAA 
CTGGAAATCA TCTTTAATGA TTTACATGAT GACTTTCACC TGACTGATAG ACAACGTTTC 
CTCATTTTGG GTAAAATCAG TGAAGGACTT GACCTGGAGC TTCAGCTTGC CAAGGTGGCT 
CTTCTCCTTT GCTATTGCAG TTTTAAAAAG ACTAACCCTG TAGCCCTGAA TTCAAGAACA 
GAGTCGGACA TCACTTTCAT GTTTCGTTTT GTAAGAGATG ATGCCAGTGG TTTGTGTTTA 
GACGAAGGCC TCGATCAATT TCTAGACCAA GACTCTGTTA TGAATCTTGC AAACTCCAGT 
AGCGGGAGCA GCTGCAGTTC TCCATTCTAC ATCGATGAGG AGTCTCCGAT GTTTGGTCGT 
TTCCAGCGTC CTCCCAGAGT TCAGTTTCAA GAGCTTTGCA CAGTGGCTTC CAGCTCAGAC 
AGCTCTCCGA TGCAGGATTT GATGAGCACG CCACACTTCC AGTTGAAGAA ACTCCGCAAA 
GAACTGGCGC ATGAAGGAGA CGTGAGAGAT GAGCTGGAGA AAGAGCTGGC CGATCACATC 
AGCATCATCT CAGAGAAAGA GGGTCTGATC TCCCAGTTAC AGCACCGTGT GGAGCGGATG 
CTGCGGGAAC AAGGAGAGCT GGAGAAAGAC CATAAAGCTG CACTGCTGGA GCTCCAGGAG 
AAGAACGAGA GTCTTCTCCA CAGAGTTCAT GAGGTTTTAA AGCAGTGCCA AGATTTAAAA 
ACAGACAACT CTCAAAAAGA GAAAAAGATT GATGAGCTGA CAGAAGAGAA CGGGACTCTC 
AGTGCTCAGC TTCGTAATGT TTTCTCTCAG CTGGCGAGAG CAGAGCAGGA AGTTGCCAAA 
CTCACAGTGG TTCATGAATC TGCACAGACG GAGTGGAAAA ACAGGAAAGA GTTGCTGGAG 
AAGGAGCTGA ATGAGGCCGT CACACACAAG GAGTGTCTGA GTGAGCAGGT GCTGATACTG 
CAGGGGAAGA TTTCTGTTCT GGAGGATGAA CTGAAGAAAG CTCAGTCTCA GGAGAGAGGA 
GAGGTGCTGG GGCCCATCAT GGAGTGGGAA AAGCTCAAAC AAGAGTTGGC TGATCTCACT 
CTTCAACTCG CTCAGCTTCA GGAATCCATT TCCCACCTCG AGAAGAAAAA GGCAGAAGTT 
GAGGCCTTGT TGGCTGAGGA GAGATGCTCT TTTGAAAAAG AAACCGCAAG ACTCCAGTTG 
GTGGTTTTTG ATCTGGAGAA ATCCATCAAC AGCATCCGTC TGGAGAGAGA AACACTTCAG 
GAGGCCTTAA AGACGCAGAA AGAGATGCTC TCTGCGCAGA TATCAGCACT GGAGAGGGAC 
GTTTCCCGTC TGCAACAGGT TGAAGTGCAG CTGACAGCAG AGATCAAAAT ATCTGCAGAT 
CTCCGCCAAC AGAGAGAGGA GCTGGAAGGA AAGGTAGCAT CTTTAGATAG GATGGTTCAT 
TCTCTGCATG CTGAGATCCA GGGGCTGGAA GTGGAGAGAG CTTCTCAACA GGATGCAATT 
AATTCCCTCA TTAGCGATTT ACAAATCGCT AGGGCTACTG TTCAGGAATA TGAGAAGAAG 
CTGGAAGAGC ATCAAAAAGT GGTAGCAGAA AACAATTCCC TGAAAAATGA AGCATGCACA 
ATGCAGCAGG AGCTGAATAA TCATCTAAAG GCAATCGGAG ACCTTCAGGC GGAAGTAAAT 
GAACTCAGAT TTGAGAAAAC TGAGGAAGAG AACAAGGTTA GTGAGGCTTT GGTTAAAATC 
GAAAGCCTCC AGACTGAGAT TCTGCATCTG TGTGAAAAGA TTTCTCTTAA AGATGAAGAA 
ATCAGAAACC TGACGAAAGA GTATGAGTCT GTAGACAACG AGCTGAAGCT TGTGAAGGAG 
CAAAATGTTG AAATAAATGC AATGATCAAA TCTAATCGCA AGGAGCATGA GGAAACTGTT 
GAAAAACTTC AGCAGGAGCT TCATTGCGCT GCTTCTGCCG CATCAGAAAA ACAAGAGCAA 
ATGCTGGTTT TATCAGCAGA GGTAACATCT CTGAAAGAGC AAATCTGCAG ATATAGTGAA 
AATGAGGCAC AGAAACAACA GGAATTGTCT ATTTTAGAAG CACAACACAA TGTGCTGAAG 
GAAAACCTGA CTTCTCTACA GAACCAGTTG GCTGAGGCGA CAACTTCAGC TTCACAGAAG 
GAATCAGAGT TTATTTTGCT TCAACAGGAG CTTTCCCATC AAGAAACACT AAGAGAACAA 
ACTCAGGAGC TTGAGAAGAT TAAACGTGAG GAGCTTGAGG GGATCGTGAG TGAACTTCAG 
GCCAAAATTA TTGAGGTTTC CTCTATTGCC TCAGAAAGGG AAGCTTGTGT GAGCTCCCTT 
CAATATGAGA TGAAAGATCA GCTATCGAAA GCCAAACAGT GTGAAGCTGA TCTACGCAAT 
GAGATGAAGG AAAAGGTTGG AAATTTGCAG GAACTTCTAG ACGCTGCTAA CCATGGAATT 
GCTGAAAAAG AGCATCACTT AGAGTCGTTG AATCAAAGTC TGAAGCAGAT GGAGCTTCTG 
TACTGTCAAA AGGAGGAAGA AATCATTGCA AGTCAACAAG CAAAGCAGCA ACTGGAGAAT 
AATATTGCTG AGCAGAAACA ACAACTAGAT GAGATTCAGC AAAATCGAGA AATACTAAGG 
AATGAAAGAG ACCATCTGTC TACTCGAGTA TCATCTCTCC AAGAGGAAAT TCATCGTTCC 
CAACAGGATT TTTCTGATTT ACAGACTGAC CACAATGTTT TGAAGGAAAA ACTGGTTAAT 
CTTCAGGATC AAGTAGAGCA GGCGATGATT ACTGCTTCCC AGAAGGAATC TGAACTCCTC 
TTGCTTCAAC AGGAGCTTTC CCATCAAGAG ACCCTGAGAG AAAAAGCTTT GGAGCTTGAG 
GCCATCAAGC GAGAAGAGTT TGAGAAAAAA GTGAAGGAAC TGCAGGACAG AATTCTAGAG 
GTGTCAACTC TTGCCTCTCA AAGGGAAGCC CAGGTAATTT CTCTTGAAAA TGAGATGAGG 
GATCAACAGT TGAGTGCCAA CCAATCTAAA GATAATCTCT GCAGGGAGTG GCAGGAGAAG 
CTTGGCATCT TGAAAGGAGA ATTAGATGTA GTCAGTCGAG ACGCTGCAGA CAAAGACCAT 
CAACTGGAAT CTTTGGATCA AAAGCTTAAA GAGATGGAAA TGGTTGTCCT TCAAAAAGAG 
AAAGATGTAA TGGAGACACA TCAGGCAAAG GAGGACCTGG AGAAGAGAAT TGCTGAGCTT 
GAGGAGTGCA AGCAGAAATT AGAGATTATG AGGAATGAAA GAGATCATCT TTCAACTGAA 
GTAGCATCTC TAAAAGAGGA GATTCATAGC TACCAGGATA CTCAAATGCA GAAACAACAA 
ACAATTTCTG TTTTGGAAGT TGAAAACAAT GCTTTAAAGG AAAACATGGC TGCCCTTGAG 
AAACAATTAG CAGAGGAGAT AACTACAGCT TCACAGAAAA ATTCAGAGCT TCAAAACAAG 
CTTCATCAAC AAGAGAGCCT TCACGAAAAG GCTCAAGAGC TTGAGATTGG CAGGCATGAG 
GAATTTGAGA GAAGAGTGAG AGAACTTCAA GCTAAAGTCC AAGAGGTGTC CACTCTTGCT 
TCTGAGAAGG AGGCTGTTGC TTATTCTCTT CAGGAGGAAT TGACGGGTCA TTTGAAGGCT 
AAAGTGTCCG AAGATGATCT CCGTAGAGTC CTGGAGGAAA AAGTGGAAAC CTTACAGAGA 
GAAATAGAAA CTGCTAGTTG TGATGCCACA AGCAAAGACG GTCTTTTGCA GACTTTGGAT 
CAGAAGCTGA GGCAGATGGA GATGCTTTGC CAACAAAAGG AGGATGCAGT TTTTGAGATC 
CAGAACTCAA AAGAAGACTT GCAGAAAGAG ATGAATGAAC TTGTTTCTAA GAATCAAGAA 
CTGGAGGGCT GCCTGCAACA TTTGGAAATG GTAAAAAAGG AAAAAGACCT CCTGTCAAAT 
GAAGTAACTT CTCTGAAAGA GCAAATAAAT GATCAGAGTT TGAGGGCCAA ACAGTCTGAA 
GCTGATCTTT GCATAGTCTT TGAGGAAAAG ATTGAAACCC TTCAAGGACA ACTGGAATCT 
AGTAGTTGTG ACGTCTCGGA CAAAGAGAAA CATCTGCAAA CTTTGCATCA AAAGGTTTCA 
CAGATGGATC TGTTGTGCCA GCAAAAGGAG AATGCAGTTC TTGAGATGCA GAACGCAAAG 
GAAGACTTGC AGAAGAAGAT TGATGAGCTT GTTTCTGAGA AACAACAACT GGAGGGCTGC 
CTGCAAAATT TGGAAATGGT GAAAAGCGAA AGAGACCTCT TGTCAACTGA AGTAACTTCT 
CTAAAAGATC AACTAAATGA TCAGGATTTG AAAGCCAAAC AGTCCGAAGA TGATCTCCGC 
AAAGTGTTGG AAGAAAAGAT TAAAAACCTC CAGGGACAAC TTGAAACTGC TAGTCGTGAT 
GTCTCCGAAA AAGACCAGCT TCTGCAGACT TTGGATCAAA AGGTTACACA GATGGATCAG 
TTGTGTAAGC AAAAGGAGAA ATATGCTCTG GATTTGCTTC AGGTAAAGGA GAATTTGGAG 
AAGAGGATTG GTGAGATTAC TGTTGAGAAA CAGCAATTGG AAGGATGCCA GCAGAATTTG 
GAAACAGTGA GCAAGGACAA AGACAACCTT TTAAATGAAT TAACCTTTCT TAAAGTTGAA 
ATCCAAAGTT ACCAGGAAAA AGAAGTGCAG ATGAAACATG AGCTTTCTGT TTTAGAGAAT 
GAACACAACA TTTTACAGGA GAACCTGGAT ACTCTTCAGA AGCAAGTGGT GGAGTTGACC 
GCTTCAGCTT CTCAGAAAGA GTCTGAGCTT CAAAATGAGG TTTGCAAACA AGAGAAACTT 
CAAGAAAAGG CTCAGAAACT TGAGAAGGAT GCAGGTGATC TTCAGGCTAA AATTCTAGAG 
ATTTCCACTC TTGCTTCTGA GAGAGAGGCA CAAATAAGTT CTCTTAAAGA TGAAATAAAC 
AGTCAGCATT TGAAAGCTAA ACAGTCTGAA GATGATCTCC TCAGAGTGTT TGAGGAGAAA 
ATTGAAAACC TTCAAGGACA GCTTGAAATT GCTCGTCTTG ATGTCTCGGA CAAAGACCAG 
CTTCTGCAAA CTTTAAATCA GAAACTGAAA CAAATGGAAG AACTATGCAA GCAAAAGGAG 
ACATGTGTCC TTGAGATGCA TCAGACAAAG GAGGATCTGG AGAAGAAAAC TGTTGAGCTT 
CAAGCTGACA AAAGTCAACA GAATCAGCAG AATTTGGAGA TGTTGAGGCA AGAAAAGGAC 
CATCTGAAAT CTCTAAACCA GACTATCCGA AGAGAGTGTG AAGCGTCCCG GAAGATTCGA 
GCTGAGCTGG AGTTGAACGT TGAGGAACAA ACTGCTTCCA TCCTTGCACT TAAAAAGGCT 
TCCCAACAGT GGGAGGAGCA GAATCAGGAG CTCCTGGAGC AGCTGAAGGC AAAGACAGAA 
GCTGTAGAGC ACTACAAAGC ACAGGTGGAG AAGGCCATGA ATCACTACAA TGGTAAAAAG 
CAGCTGCTGC TGGAGGCTCA AGAGCTGAAC AAAACTCTTG AGCAGTCATT GGAGGTCAGC 
AAGAGAGAAG CTAAAGCTCT GGAAACTGAA CTAACCCTGG CTCGCATGGA GCTCAACCAA 
GCCAATGATA AAGAGAAGAG CCTTGCTGCT AAAGTGAAGA CTTTGGAGGC CCAAGTTGAT 
TTTGCTGACA GGCAACTGCG AGAGAAGAGA AGGATTGCAG ACGATGGTGA GGATTTGAGA 
CAAAAAGAAA ATCTGTACTT CAGAGTCCCA GAGAAGCAGC AAGACACCAG TGCAGACAGC 
CTGGAGTTTG AGCTAAACGA TTCTCTCAAC ACTACCTGTC GTTCTGCAGT ACCGGGTGAG 
TCCAGCACTC CACTGGTCCG TAGTTCTGAG CGTTTGGCTG CTAAACGCCG CGCATTGGGG 
GCTGAATCTC TCGAGACACT ATATTTCACA CCTATGGGTA AACAAGGCTA CAAACGCAAA 
GTGGATCACA ATGATGGTTT TGAACGCAAA CTGGAGTCCA GCATCACTTC TATTGGTGAT 
CTCGTGGTTG ATTCTGCAAA GAAATTGACT GCTTCAGCTC GCCGGCGACG CACTACACAA 
GTTATCAACA TCACCTTGGC TAAGAAGGCA CAAGGAAGCT CTGAAAGCGA GGAGTCGTTT 
TCTAGCCTGC ACTCAGCCCG TTCTCATCCA AATTTGGCAG TCCACCAATC ACGGCCGATC 
TCCATTGACT TCTCAGAGGA TGGTCCTGCC ACAACGTTAT CAAAAGCAGA CACACTTCAA 
AATCTGCCTG GATATCGCAG AAGTACAGTG CACAGCGTTG GCCCACCAAG AGCCACCAGT 
ACTTTTTGTA TTGGAGCAGA GAATGAACCG GAGCATGCTG CCGATGACTG GATGCGCATC 
GCTGAACTGC AGTCCCGAAA CAAAGCCTGT CTGCCTCATC TGAAGAGCAG CTATCCTCTG 
GAGTCCAGGC CCAGTCTAGG ACCATCATTC ACTGTAACGG ATGAGGACCT GCGCTTGGGC 
GACCCGAGTG AGACGATCCG GCGTGCGTCC ATGATGCCGG GTCAGATCCT GGAGTCGCTG 
AACTCTCGCC GTTTCTCCCT CGCTCCTGAA ACGAGTTCGA GCCAGGCTCC TGCACGCTCT 
CATCCCCAGC GAGCCACCAT GCTGCCTTGT CAGATCCAAT CCAGCACTGC TGCTCACCGG 
GCCTCACAGC TCACAAAAAC AAGCTCACGT TCACACACTT CAGAGAATAA ACGCAGCCCG 
CTGGCTCCCA AACGCCCGGC ATCACAACTG CAAGGACCAG ACACTCCTGA GGCAAAGAAG 
TTGGCAAGTT GTTTCCCACG GCCAATGACG CCCAAAGGCC GGTTTGGAAA CAGCCAAAAC 
AGACCTCCAG TCTCTCCGGC TGAGCGAAGA CAGTCTGTGA TGTTTACTGT GGTAAATACT 
CCAAAGAACA GTGCTCGTGG AGACAGCAGG CTTCAGCGCG GCCTCAACAA GCTGCGTAAC 
AGCACCCGCA AATCTCCTGC CGTGGCCAGT CGGGCTTTGC GCTCGGCCGT GAGCGGGAAA 
TCTCCCCTGG ACGTCACTCT GAGGAAATCT CCCCGAAACA AGTCCCCGAA AACATCCAAT 
GCCAAAAAGG AAACACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_009291967.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009290242.1 Danio rerio nuclear mitotic apparatus protein 1 (numa1), transcript variant X2, mRNA.

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CloneID	ODa46286
Clone ID Related Accession (Same CDS sequence)	XM_009291966.3 , XM_009291967.3 , XM_009291967.2 , XM_009291966.2
Accession Version	XM_009291967.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291967.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTTA ATAAGAACAA GGCGGGACCA CTTTTGACAT GGATGAACAG CGTGTTTCCA 
GAGAATCAAA TCTGGGAGTT TGGTCATATG AGAGACGGCT TTCGACTCTT GGAGATCTGC 
TTCAAACTAA AGGGGAGGGA GGACGTTGAA GCTTATAAGA CTTTATCCTT ATACAACAAA 
CTGGAAATCA TCTTTAATGA TTTACATGAT GACTTTCACC TGACTGATAG ACAACGTTTC 
CTCATTTTGG GTAAAATCAG TGAAGGACTT GACCTGGAGC TTCAGCTTGC CAAGGTGGCT 
CTTCTCCTTT GCTATTGCAG TTTTAAAAAG ACTAACCCTG TAGCCCTGAA TTCAAGAACA 
GAGTCGGACA TCACTTTCAT GTTTCGTTTT GTAAGAGATG ATGCCAGTGG TTTGTGTTTA 
GACGAAGGCC TCGATCAATT TCTAGACCAA GACTCTGTTA TGAATCTTGC AAACTCCAGT 
AGCGGGAGCA GCTGCAGTTC TCCATTCTAC ATCGATGAGG AGTCTCCGAT GTTTGGTCGT 
TTCCAGCGTC CTCCCAGAGT TCAGTTTCAA GAGCTTTGCA CAGTGGCTTC CAGCTCAGAC 
AGCTCTCCGA TGCAGGATTT GATGAGCACG CCACACTTCC AGTTGAAGAA ACTCCGCAAA 
GAACTGGCGC ATGAAGGAGA CGTGAGAGAT GAGCTGGAGA AAGAGCTGGC CGATCACATC 
AGCATCATCT CAGAGAAAGA GGGTCTGATC TCCCAGTTAC AGCACCGTGT GGAGCGGATG 
CTGCGGGAAC AAGGAGAGCT GGAGAAAGAC CATAAAGCTG CACTGCTGGA GCTCCAGGAG 
AAGAACGAGA GTCTTCTCCA CAGAGTTCAT GAGGTTTTAA AGCAGTGCCA AGATTTAAAA 
ACAGACAACT CTCAAAAAGA GAAAAAGATT GATGAGCTGA CAGAAGAGAA CGGGACTCTC 
AGTGCTCAGC TTCGTAATGT TTTCTCTCAG CTGGCGAGAG CAGAGCAGGA AGTTGCCAAA 
CTCACAGTGG TTCATGAATC TGCACAGACG GAGTGGAAAA ACAGGAAAGA GTTGCTGGAG 
AAGGAGCTGA ATGAGGCCGT CACACACAAG GAGTGTCTGA GTGAGCAGGT GCTGATACTG 
CAGGGGAAGA TTTCTGTTCT GGAGGATGAA CTGAAGAAAG CTCAGTCTCA GGAGAGAGGA 
GAGGTGCTGG GGCCCATCAT GGAGTGGGAA AAGCTCAAAC AAGAGTTGGC TGATCTCACT 
CTTCAACTCG CTCAGCTTCA GGAATCCATT TCCCACCTCG AGAAGAAAAA GGCAGAAGTT 
GAGGCCTTGT TGGCTGAGGA GAGATGCTCT TTTGAAAAAG AAACCGCAAG ACTCCAGTTG 
GTGGTTTTTG ATCTGGAGAA ATCCATCAAC AGCATCCGTC TGGAGAGAGA AACACTTCAG 
GAGGCCTTAA AGACGCAGAA AGAGATGCTC TCTGCGCAGA TATCAGCACT GGAGAGGGAC 
GTTTCCCGTC TGCAACAGGT TGAAGTGCAG CTGACAGCAG AGATCAAAAT ATCTGCAGAT 
CTCCGCCAAC AGAGAGAGGA GCTGGAAGGA AAGGTAGCAT CTTTAGATAG GATGGTTCAT 
TCTCTGCATG CTGAGATCCA GGGGCTGGAA GTGGAGAGAG CTTCTCAACA GGATGCAATT 
AATTCCCTCA TTAGCGATTT ACAAATCGCT AGGGCTACTG TTCAGGAATA TGAGAAGAAG 
CTGGAAGAGC ATCAAAAAGT GGTAGCAGAA AACAATTCCC TGAAAAATGA AGCATGCACA 
ATGCAGCAGG AGCTGAATAA TCATCTAAAG GCAATCGGAG ACCTTCAGGC GGAAGTAAAT 
GAACTCAGAT TTGAGAAAAC TGAGGAAGAG AACAAGGTTA GTGAGGCTTT GGTTAAAATC 
GAAAGCCTCC AGACTGAGAT TCTGCATCTG TGTGAAAAGA TTTCTCTTAA AGATGAAGAA 
ATCAGAAACC TGACGAAAGA GTATGAGTCT GTAGACAACG AGCTGAAGCT TGTGAAGGAG 
CAAAATGTTG AAATAAATGC AATGATCAAA TCTAATCGCA AGGAGCATGA GGAAACTGTT 
GAAAAACTTC AGCAGGAGCT TCATTGCGCT GCTTCTGCCG CATCAGAAAA ACAAGAGCAA 
ATGCTGGTTT TATCAGCAGA GGTAACATCT CTGAAAGAGC AAATCTGCAG ATATAGTGAA 
AATGAGGCAC AGAAACAACA GGAATTGTCT ATTTTAGAAG CACAACACAA TGTGCTGAAG 
GAAAACCTGA CTTCTCTACA GAACCAGTTG GCTGAGGCGA CAACTTCAGC TTCACAGAAG 
GAATCAGAGT TTATTTTGCT TCAACAGGAG CTTTCCCATC AAGAAACACT AAGAGAACAA 
ACTCAGGAGC TTGAGAAGAT TAAACGTGAG GAGCTTGAGG GGATCGTGAG TGAACTTCAG 
GCCAAAATTA TTGAGGTTTC CTCTATTGCC TCAGAAAGGG AAGCTTGTGT GAGCTCCCTT 
CAATATGAGA TGAAAGATCA GCTATCGAAA GCCAAACAGT GTGAAGCTGA TCTACGCAAT 
GAGATGAAGG AAAAGGTTGG AAATTTGCAG GAACTTCTAG ACGCTGCTAA CCATGGAATT 
GCTGAAAAAG AGCATCACTT AGAGTCGTTG AATCAAAGTC TGAAGCAGAT GGAGCTTCTG 
TACTGTCAAA AGGAGGAAGA AATCATTGCA AGTCAACAAG CAAAGCAGCA ACTGGAGAAT 
AATATTGCTG AGCAGAAACA ACAACTAGAT GAGATTCAGC AAAATCGAGA AATACTAAGG 
AATGAAAGAG ACCATCTGTC TACTCGAGTA TCATCTCTCC AAGAGGAAAT TCATCGTTCC 
CAACAGGATT TTTCTGATTT ACAGACTGAC CACAATGTTT TGAAGGAAAA ACTGGTTAAT 
CTTCAGGATC AAGTAGAGCA GGCGATGATT ACTGCTTCCC AGAAGGAATC TGAACTCCTC 
TTGCTTCAAC AGGAGCTTTC CCATCAAGAG ACCCTGAGAG AAAAAGCTTT GGAGCTTGAG 
GCCATCAAGC GAGAAGAGTT TGAGAAAAAA GTGAAGGAAC TGCAGGACAG AATTCTAGAG 
GTGTCAACTC TTGCCTCTCA AAGGGAAGCC CAGGTAATTT CTCTTGAAAA TGAGATGAGG 
GATCAACAGT TGAGTGCCAA CCAATCTAAA GATAATCTCT GCAGGGAGTG GCAGGAGAAG 
CTTGGCATCT TGAAAGGAGA ATTAGATGTA GTCAGTCGAG ACGCTGCAGA CAAAGACCAT 
CAACTGGAAT CTTTGGATCA AAAGCTTAAA GAGATGGAAA TGGTTGTCCT TCAAAAAGAG 
AAAGATGTAA TGGAGACACA TCAGGCAAAG GAGGACCTGG AGAAGAGAAT TGCTGAGCTT 
GAGGAGTGCA AGCAGAAATT AGAGATTATG AGGAATGAAA GAGATCATCT TTCAACTGAA 
GTAGCATCTC TAAAAGAGGA GATTCATAGC TACCAGGATA CTCAAATGCA GAAACAACAA 
ACAATTTCTG TTTTGGAAGT TGAAAACAAT GCTTTAAAGG AAAACATGGC TGCCCTTGAG 
AAACAATTAG CAGAGGAGAT AACTACAGCT TCACAGAAAA ATTCAGAGCT TCAAAACAAG 
CTTCATCAAC AAGAGAGCCT TCACGAAAAG GCTCAAGAGC TTGAGATTGG CAGGCATGAG 
GAATTTGAGA GAAGAGTGAG AGAACTTCAA GCTAAAGTCC AAGAGGTGTC CACTCTTGCT 
TCTGAGAAGG AGGCTGTTGC TTATTCTCTT CAGGAGGAAT TGACGGGTCA TTTGAAGGCT 
AAAGTGTCCG AAGATGATCT CCGTAGAGTC CTGGAGGAAA AAGTGGAAAC CTTACAGAGA 
GAAATAGAAA CTGCTAGTTG TGATGCCACA AGCAAAGACG GTCTTTTGCA GACTTTGGAT 
CAGAAGCTGA GGCAGATGGA GATGCTTTGC CAACAAAAGG AGGATGCAGT TTTTGAGATC 
CAGAACTCAA AAGAAGACTT GCAGAAAGAG ATGAATGAAC TTGTTTCTAA GAATCAAGAA 
CTGGAGGGCT GCCTGCAACA TTTGGAAATG GTAAAAAAGG AAAAAGACCT CCTGTCAAAT 
GAAGTAACTT CTCTGAAAGA GCAAATAAAT GATCAGAGTT TGAGGGCCAA ACAGTCTGAA 
GCTGATCTTT GCATAGTCTT TGAGGAAAAG ATTGAAACCC TTCAAGGACA ACTGGAATCT 
AGTAGTTGTG ACGTCTCGGA CAAAGAGAAA CATCTGCAAA CTTTGCATCA AAAGGTTTCA 
CAGATGGATC TGTTGTGCCA GCAAAAGGAG AATGCAGTTC TTGAGATGCA GAACGCAAAG 
GAAGACTTGC AGAAGAAGAT TGATGAGCTT GTTTCTGAGA AACAACAACT GGAGGGCTGC 
CTGCAAAATT TGGAAATGGT GAAAAGCGAA AGAGACCTCT TGTCAACTGA AGTAACTTCT 
CTAAAAGATC AACTAAATGA TCAGGATTTG AAAGCCAAAC AGTCCGAAGA TGATCTCCGC 
AAAGTGTTGG AAGAAAAGAT TAAAAACCTC CAGGGACAAC TTGAAACTGC TAGTCGTGAT 
GTCTCCGAAA AAGACCAGCT TCTGCAGACT TTGGATCAAA AGGTTACACA GATGGATCAG 
TTGTGTAAGC AAAAGGAGAA ATATGCTCTG GATTTGCTTC AGGTAAAGGA GAATTTGGAG 
AAGAGGATTG GTGAGATTAC TGTTGAGAAA CAGCAATTGG AAGGATGCCA GCAGAATTTG 
GAAACAGTGA GCAAGGACAA AGACAACCTT TTAAATGAAT TAACCTTTCT TAAAGTTGAA 
ATCCAAAGTT ACCAGGAAAA AGAAGTGCAG ATGAAACATG AGCTTTCTGT TTTAGAGAAT 
GAACACAACA TTTTACAGGA GAACCTGGAT ACTCTTCAGA AGCAAGTGGT GGAGTTGACC 
GCTTCAGCTT CTCAGAAAGA GTCTGAGCTT CAAAATGAGG TTTGCAAACA AGAGAAACTT 
CAAGAAAAGG CTCAGAAACT TGAGAAGGAT GCAGGTGATC TTCAGGCTAA AATTCTAGAG 
ATTTCCACTC TTGCTTCTGA GAGAGAGGCA CAAATAAGTT CTCTTAAAGA TGAAATAAAC 
AGTCAGCATT TGAAAGCTAA ACAGTCTGAA GATGATCTCC TCAGAGTGTT TGAGGAGAAA 
ATTGAAAACC TTCAAGGACA GCTTGAAATT GCTCGTCTTG ATGTCTCGGA CAAAGACCAG 
CTTCTGCAAA CTTTAAATCA GAAACTGAAA CAAATGGAAG AACTATGCAA GCAAAAGGAG 
ACATGTGTCC TTGAGATGCA TCAGACAAAG GAGGATCTGG AGAAGAAAAC TGTTGAGCTT 
CAAGCTGACA AAAGTCAACA GAATCAGCAG AATTTGGAGA TGTTGAGGCA AGAAAAGGAC 
CATCTGAAAT CTCTAAACCA GACTATCCGA AGAGAGTGTG AAGCGTCCCG GAAGATTCGA 
GCTGAGCTGG AGTTGAACGT TGAGGAACAA ACTGCTTCCA TCCTTGCACT TAAAAAGGCT 
TCCCAACAGT GGGAGGAGCA GAATCAGGAG CTCCTGGAGC AGCTGAAGGC AAAGACAGAA 
GCTGTAGAGC ACTACAAAGC ACAGGTGGAG AAGGCCATGA ATCACTACAA TGGTAAAAAG 
CAGCTGCTGC TGGAGGCTCA AGAGCTGAAC AAAACTCTTG AGCAGTCATT GGAGGTCAGC 
AAGAGAGAAG CTAAAGCTCT GGAAACTGAA CTAACCCTGG CTCGCATGGA GCTCAACCAA 
GCCAATGATA AAGAGAAGAG CCTTGCTGCT AAAGTGAAGA CTTTGGAGGC CCAAGTTGAT 
TTTGCTGACA GGCAACTGCG AGAGAAGAGA AGGATTGCAG ACGATGGTGA GGATTTGAGA 
CAAAAAGAAA ATCTGTACTT CAGAGTCCCA GAGAAGCAGC AAGACACCAG TGCAGACAGC 
CTGGAGTTTG AGCTAAACGA TTCTCTCAAC ACTACCTGTC GTTCTGCAGT ACCGGGTGAG 
TCCAGCACTC CACTGGTCCG TAGTTCTGAG CGTTTGGCTG CTAAACGCCG CGCATTGGGG 
GCTGAATCTC TCGAGACACT ATATTTCACA CCTATGGGTA AACAAGGCTA CAAACGCAAA 
GTGGATCACA ATGATGGTTT TGAACGCAAA CTGGAGTCCA GCATCACTTC TATTGGTGAT 
CTCGTGGTTG ATTCTGCAAA GAAATTGACT GCTTCAGCTC GCCGGCGACG CACTACACAA 
GTTATCAACA TCACCTTGGC TAAGAAGGCA CAAGGAAGCT CTGAAAGCGA GGAGTCGTTT 
TCTAGCCTGC ACTCAGCCCG TTCTCATCCA AATTTGGCAG TCCACCAATC ACGGCCGATC 
TCCATTGACT TCTCAGAGGA TGGTCCTGCC ACAACGTTAT CAAAAGCAGA CACACTTCAA 
AATCTGCCTG GATATCGCAG AAGTACAGTG CACAGCGTTG GCCCACCAAG AGCCACCAGT 
ACTTTTTGTA TTGGAGCAGA GAATGAACCG GAGCATGCTG CCGATGACTG GATGCGCATC 
GCTGAACTGC AGTCCCGAAA CAAAGCCTGT CTGCCTCATC TGAAGAGCAG CTATCCTCTG 
GAGTCCAGGC CCAGTCTAGG ACCATCATTC ACTGTAACGG ATGAGGACCT GCGCTTGGGC 
GACCCGAGTG AGACGATCCG GCGTGCGTCC ATGATGCCGG GTCAGATCCT GGAGTCGCTG 
AACTCTCGCC GTTTCTCCCT CGCTCCTGAA ACGAGTTCGA GCCAGGCTCC TGCACGCTCT 
CATCCCCAGC GAGCCACCAT GCTGCCTTGT CAGATCCAAT CCAGCACTGC TGCTCACCGG 
GCCTCACAGC TCACAAAAAC AAGCTCACGT TCACACACTT CAGAGAATAA ACGCAGCCCG 
CTGGCTCCCA AACGCCCGGC ATCACAACTG CAAGGACCAG ACACTCCTGA GGCAAAGAAG 
TTGGCAAGTT GTTTCCCACG GCCAATGACG CCCAAAGGCC GGTTTGGAAA CAGCCAAAAC 
AGACCTCCAG TCTCTCCGGC TGAGCGAAGA CAGTCTGTGA TGTTTACTGT GGTAAATACT 
CCAAAGAACA GTGCTCGTGG AGACAGCAGG CTTCAGCGCG GCCTCAACAA GCTGCGTAAC 
AGCACCCGCA AATCTCCTGC CGTGGCCAGT CGGGCTTTGC GCTCGGCCGT GAGCGGGAAA 
TCTCCCCTGG ACGTCACTCT GAGGAAATCT CCCCGAAACA AGTCCCCGAA AACATCCAAT 
GCCAAAAAGG AAACACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290242.1
CDS	75..7415
Translation	MKFNKNKAGPLLTWMNSVFPENQIWEFGHMRDGFRLLEICFKLKGREDVEAYKTLSLYNKLEIIFNDLHDDFHLTDRQRFLILGKISEGLDLELQLAKVALLLCYCSFKKTNPVALNSRTESDITFMFRFVRDDASGLCLDEGLDQFLDQDSVMNLANSSSGSSCSSPFYIDEESPMFGRFQRPPRVQFQELCTVASSSDSSPMQDLMSTPHFQLKKLRKELAHEGDVRDELEKELADHISIISEKEGLISQLQHRVERMLREQGELEKDHKAALLELQEKNESLLHRVHEVLKQCQDLKTDNSQKEKKIDELTEENGTLSAQLRNVFSQLARAEQEVAKLTVVHESAQTEWKNRKELLEKELNEAVTHKECLSEQVLILQGKISVLEDELKKAQSQERGEVLGPIMEWEKLKQELADLTLQLAQLQESISHLEKKKAEVEALLAEERCSFEKETARLQLVVFDLEKSINSIRLERETLQEALKTQKEMLSAQISALERDVSRLQQVEVQLTAEIKISADLRQQREELEGKVASLDRMVHSLHAEIQGLEVERASQQDAINSLISDLQIARATVQEYEKKLEEHQKVVAENNSLKNEACTMQQELNNHLKAIGDLQAEVNELRFEKTEEENKVSEALVKIESLQTEILHLCEKISLKDEEIRNLTKEYESVDNELKLVKEQNVEINAMIKSNRKEHEETVEKLQQELHCAASAASEKQEQMLVLSAEVTSLKEQICRYSENEAQKQQELSILEAQHNVLKENLTSLQNQLAEATTSASQKESEFILLQQELSHQETLREQTQELEKIKREELEGIVSELQAKIIEVSSIASEREACVSSLQYEMKDQLSKAKQCEADLRNEMKEKVGNLQELLDAANHGIAEKEHHLESLNQSLKQMELLYCQKEEEIIASQQAKQQLENNIAEQKQQLDEIQQNREILRNERDHLSTRVSSLQEEIHRSQQDFSDLQTDHNVLKEKLVNLQDQVEQAMITASQKESELLLLQQELSHQETLREKALELEAIKREEFEKKVKELQDRILEVSTLASQREAQVISLENEMRDQQLSANQSKDNLCREWQEKLGILKGELDVVSRDAADKDHQLESLDQKLKEMEMVVLQKEKDVMETHQAKEDLEKRIAELEECKQKLEIMRNERDHLSTEVASLKEEIHSYQDTQMQKQQTISVLEVENNALKENMAALEKQLAEEITTASQKNSELQNKLHQQESLHEKAQELEIGRHEEFERRVRELQAKVQEVSTLASEKEAVAYSLQEELTGHLKAKVSEDDLRRVLEEKVETLQREIETASCDATSKDGLLQTLDQKLRQMEMLCQQKEDAVFEIQNSKEDLQKEMNELVSKNQELEGCLQHLEMVKKEKDLLSNEVTSLKEQINDQSLRAKQSEADLCIVFEEKIETLQGQLESSSCDVSDKEKHLQTLHQKVSQMDLLCQQKENAVLEMQNAKEDLQKKIDELVSEKQQLEGCLQNLEMVKSERDLLSTEVTSLKDQLNDQDLKAKQSEDDLRKVLEEKIKNLQGQLETASRDVSEKDQLLQTLDQKVTQMDQLCKQKEKYALDLLQVKENLEKRIGEITVEKQQLEGCQQNLETVSKDKDNLLNELTFLKVEIQSYQEKEVQMKHELSVLENEHNILQENLDTLQKQVVELTASASQKESELQNEVCKQEKLQEKAQKLEKDAGDLQAKILEISTLASEREAQISSLKDEINSQHLKAKQSEDDLLRVFEEKIENLQGQLEIARLDVSDKDQLLQTLNQKLKQMEELCKQKETCVLEMHQTKEDLEKKTVELQADKSQQNQQNLEMLRQEKDHLKSLNQTIRRECEASRKIRAELELNVEEQTASILALKKASQQWEEQNQELLEQLKAKTEAVEHYKAQVEKAMNHYNGKKQLLLEAQELNKTLEQSLEVSKREAKALETELTLARMELNQANDKEKSLAAKVKTLEAQVDFADRQLREKRRIADDGEDLRQKENLYFRVPEKQQDTSADSLEFELNDSLNTTCRSAVPGESSTPLVRSSERLAAKRRALGAESLETLYFTPMGKQGYKRKVDHNDGFERKLESSITSIGDLVVDSAKKLTASARRRRTTQVINITLAKKAQGSSESEESFSSLHSARSHPNLAVHQSRPISIDFSEDGPATTLSKADTLQNLPGYRRSTVHSVGPPRATSTFCIGAENEPEHAADDWMRIAELQSRNKACLPHLKSSYPLESRPSLGPSFTVTDEDLRLGDPSETIRRASMMPGQILESLNSRRFSLAPETSSSQAPARSHPQRATMLPCQIQSSTAAHRASQLTKTSSRSHTSENKRSPLAPKRPASQLQGPDTPEAKKLASCFPRPMTPKGRFGNSQNRPPVSPAERRQSVMFTVVNTPKNSARGDSRLQRGLNKLRNSTRKSPAVASRALRSAVSGKSPLDVTLRKSPRNKSPKTSNAKKETQ

Target ORF information:

RefSeq Version	XM_009291967.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nuclear mitotic apparatus protein 1 (numa1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291967.3

ORF Insert Sequence:

ATGAAGTTTA ATAAGAACAA GGCGGGACCA CTTTTGACAT GGATGAACAG CGTGTTTCCA 
GAGAATCAAA TCTGGGAGTT TGGTCATATG AGAGACGGCT TTCGACTCTT GGAGATCTGC 
TTCAAACTAA AGGGGAGGGA GGACGTTGAA GCTTATAAGA CTTTATCCTT ATACAACAAA 
CTGGAAATCA TCTTTAATGA TTTACATGAT GACTTTCACC TGACTGATAG ACAACGTTTC 
CTCATTTTGG GTAAAATCAG TGAAGGACTT GACCTGGAGC TTCAGCTTGC CAAGGTGGCT 
CTTCTCCTTT GCTATTGCAG TTTTAAAAAG ACTAACCCTG TAGCCCTGAA TTCAAGAACA 
GAGTCGGACA TCACTTTCAT GTTTCGTTTT GTAAGAGATG ATGCCAGTGG TTTGTGTTTA 
GACGAAGGCC TCGATCAATT TCTAGACCAA GACTCTGTTA TGAATCTTGC AAACTCCAGT 
AGCGGGAGCA GCTGCAGTTC TCCATTCTAC ATCGATGAGG AGTCTCCGAT GTTTGGTCGT 
TTCCAGCGTC CTCCCAGAGT TCAGTTTCAA GAGCTTTGCA CAGTGGCTTC CAGCTCAGAC 
AGCTCTCCGA TGCAGGATTT GATGAGCACG CCACACTTCC AGTTGAAGAA ACTCCGCAAA 
GAACTGGCGC ATGAAGGAGA CGTGAGAGAT GAGCTGGAGA AAGAGCTGGC CGATCACATC 
AGCATCATCT CAGAGAAAGA GGGTCTGATC TCCCAGTTAC AGCACCGTGT GGAGCGGATG 
CTGCGGGAAC AAGGAGAGCT GGAGAAAGAC CATAAAGCTG CACTGCTGGA GCTCCAGGAG 
AAGAACGAGA GTCTTCTCCA CAGAGTTCAT GAGGTTTTAA AGCAGTGCCA AGATTTAAAA 
ACAGACAACT CTCAAAAAGA GAAAAAGATT GATGAGCTGA CAGAAGAGAA CGGGACTCTC 
AGTGCTCAGC TTCGTAATGT TTTCTCTCAG CTGGCGAGAG CAGAGCAGGA AGTTGCCAAA 
CTCACAGTGG TTCATGAATC TGCACAGACG GAGTGGAAAA ACAGGAAAGA GTTGCTGGAG 
AAGGAGCTGA ATGAGGCCGT CACACACAAG GAGTGTCTGA GTGAGCAGGT GCTGATACTG 
CAGGGGAAGA TTTCTGTTCT GGAGGATGAA CTGAAGAAAG CTCAGTCTCA GGAGAGAGGA 
GAGGTGCTGG GGCCCATCAT GGAGTGGGAA AAGCTCAAAC AAGAGTTGGC TGATCTCACT 
CTTCAACTCG CTCAGCTTCA GGAATCCATT TCCCACCTCG AGAAGAAAAA GGCAGAAGTT 
GAGGCCTTGT TGGCTGAGGA GAGATGCTCT TTTGAAAAAG AAACCGCAAG ACTCCAGTTG 
GTGGTTTTTG ATCTGGAGAA ATCCATCAAC AGCATCCGTC TGGAGAGAGA AACACTTCAG 
GAGGCCTTAA AGACGCAGAA AGAGATGCTC TCTGCGCAGA TATCAGCACT GGAGAGGGAC 
GTTTCCCGTC TGCAACAGGT TGAAGTGCAG CTGACAGCAG AGATCAAAAT ATCTGCAGAT 
CTCCGCCAAC AGAGAGAGGA GCTGGAAGGA AAGGTAGCAT CTTTAGATAG GATGGTTCAT 
TCTCTGCATG CTGAGATCCA GGGGCTGGAA GTGGAGAGAG CTTCTCAACA GGATGCAATT 
AATTCCCTCA TTAGCGATTT ACAAATCGCT AGGGCTACTG TTCAGGAATA TGAGAAGAAG 
CTGGAAGAGC ATCAAAAAGT GGTAGCAGAA AACAATTCCC TGAAAAATGA AGCATGCACA 
ATGCAGCAGG AGCTGAATAA TCATCTAAAG GCAATCGGAG ACCTTCAGGC GGAAGTAAAT 
GAACTCAGAT TTGAGAAAAC TGAGGAAGAG AACAAGGTTA GTGAGGCTTT GGTTAAAATC 
GAAAGCCTCC AGACTGAGAT TCTGCATCTG TGTGAAAAGA TTTCTCTTAA AGATGAAGAA 
ATCAGAAACC TGACGAAAGA GTATGAGTCT GTAGACAACG AGCTGAAGCT TGTGAAGGAG 
CAAAATGTTG AAATAAATGC AATGATCAAA TCTAATCGCA AGGAGCATGA GGAAACTGTT 
GAAAAACTTC AGCAGGAGCT TCATTGCGCT GCTTCTGCCG CATCAGAAAA ACAAGAGCAA 
ATGCTGGTTT TATCAGCAGA GGTAACATCT CTGAAAGAGC AAATCTGCAG ATATAGTGAA 
AATGAGGCAC AGAAACAACA GGAATTGTCT ATTTTAGAAG CACAACACAA TGTGCTGAAG 
GAAAACCTGA CTTCTCTACA GAACCAGTTG GCTGAGGCGA CAACTTCAGC TTCACAGAAG 
GAATCAGAGT TTATTTTGCT TCAACAGGAG CTTTCCCATC AAGAAACACT AAGAGAACAA 
ACTCAGGAGC TTGAGAAGAT TAAACGTGAG GAGCTTGAGG GGATCGTGAG TGAACTTCAG 
GCCAAAATTA TTGAGGTTTC CTCTATTGCC TCAGAAAGGG AAGCTTGTGT GAGCTCCCTT 
CAATATGAGA TGAAAGATCA GCTATCGAAA GCCAAACAGT GTGAAGCTGA TCTACGCAAT 
GAGATGAAGG AAAAGGTTGG AAATTTGCAG GAACTTCTAG ACGCTGCTAA CCATGGAATT 
GCTGAAAAAG AGCATCACTT AGAGTCGTTG AATCAAAGTC TGAAGCAGAT GGAGCTTCTG 
TACTGTCAAA AGGAGGAAGA AATCATTGCA AGTCAACAAG CAAAGCAGCA ACTGGAGAAT 
AATATTGCTG AGCAGAAACA ACAACTAGAT GAGATTCAGC AAAATCGAGA AATACTAAGG 
AATGAAAGAG ACCATCTGTC TACTCGAGTA TCATCTCTCC AAGAGGAAAT TCATCGTTCC 
CAACAGGATT TTTCTGATTT ACAGACTGAC CACAATGTTT TGAAGGAAAA ACTGGTTAAT 
CTTCAGGATC AAGTAGAGCA GGCGATGATT ACTGCTTCCC AGAAGGAATC TGAACTCCTC 
TTGCTTCAAC AGGAGCTTTC CCATCAAGAG ACCCTGAGAG AAAAAGCTTT GGAGCTTGAG 
GCCATCAAGC GAGAAGAGTT TGAGAAAAAA GTGAAGGAAC TGCAGGACAG AATTCTAGAG 
GTGTCAACTC TTGCCTCTCA AAGGGAAGCC CAGGTAATTT CTCTTGAAAA TGAGATGAGG 
GATCAACAGT TGAGTGCCAA CCAATCTAAA GATAATCTCT GCAGGGAGTG GCAGGAGAAG 
CTTGGCATCT TGAAAGGAGA ATTAGATGTA GTCAGTCGAG ACGCTGCAGA CAAAGACCAT 
CAACTGGAAT CTTTGGATCA AAAGCTTAAA GAGATGGAAA TGGTTGTCCT TCAAAAAGAG 
AAAGATGTAA TGGAGACACA TCAGGCAAAG GAGGACCTGG AGAAGAGAAT TGCTGAGCTT 
GAGGAGTGCA AGCAGAAATT AGAGATTATG AGGAATGAAA GAGATCATCT TTCAACTGAA 
GTAGCATCTC TAAAAGAGGA GATTCATAGC TACCAGGATA CTCAAATGCA GAAACAACAA 
ACAATTTCTG TTTTGGAAGT TGAAAACAAT GCTTTAAAGG AAAACATGGC TGCCCTTGAG 
AAACAATTAG CAGAGGAGAT AACTACAGCT TCACAGAAAA ATTCAGAGCT TCAAAACAAG 
CTTCATCAAC AAGAGAGCCT TCACGAAAAG GCTCAAGAGC TTGAGATTGG CAGGCATGAG 
GAATTTGAGA GAAGAGTGAG AGAACTTCAA GCTAAAGTCC AAGAGGTGTC CACTCTTGCT 
TCTGAGAAGG AGGCTGTTGC TTATTCTCTT CAGGAGGAAT TGACGGGTCA TTTGAAGGCT 
AAAGTGTCCG AAGATGATCT CCGTAGAGTC CTGGAGGAAA AAGTGGAAAC CTTACAGAGA 
GAAATAGAAA CTGCTAGTTG TGATGCCACA AGCAAAGACG GTCTTTTGCA GACTTTGGAT 
CAGAAGCTGA GGCAGATGGA GATGCTTTGC CAACAAAAGG AGGATGCAGT TTTTGAGATC 
CAGAACTCAA AAGAAGACTT GCAGAAAGAG ATGAATGAAC TTGTTTCTAA GAATCAAGAA 
CTGGAGGGCT GCCTGCAACA TTTGGAAATG GTAAAAAAGG AAAAAGACCT CCTGTCAAAT 
GAAGTAACTT CTCTGAAAGA GCAAATAAAT GATCAGAGTT TGAGGGCCAA ACAGTCTGAA 
GCTGATCTTT GCATAGTCTT TGAGGAAAAG ATTGAAACCC TTCAAGGACA ACTGGAATCT 
AGTAGTTGTG ACGTCTCGGA CAAAGAGAAA CATCTGCAAA CTTTGCATCA AAAGGTTTCA 
CAGATGGATC TGTTGTGCCA GCAAAAGGAG AATGCAGTTC TTGAGATGCA GAACGCAAAG 
GAAGACTTGC AGAAGAAGAT TGATGAGCTT GTTTCTGAGA AACAACAACT GGAGGGCTGC 
CTGCAAAATT TGGAAATGGT GAAAAGCGAA AGAGACCTCT TGTCAACTGA AGTAACTTCT 
CTAAAAGATC AACTAAATGA TCAGGATTTG AAAGCCAAAC AGTCCGAAGA TGATCTCCGC 
AAAGTGTTGG AAGAAAAGAT TAAAAACCTC CAGGGACAAC TTGAAACTGC TAGTCGTGAT 
GTCTCCGAAA AAGACCAGCT TCTGCAGACT TTGGATCAAA AGGTTACACA GATGGATCAG 
TTGTGTAAGC AAAAGGAGAA ATATGCTCTG GATTTGCTTC AGGTAAAGGA GAATTTGGAG 
AAGAGGATTG GTGAGATTAC TGTTGAGAAA CAGCAATTGG AAGGATGCCA GCAGAATTTG 
GAAACAGTGA GCAAGGACAA AGACAACCTT TTAAATGAAT TAACCTTTCT TAAAGTTGAA 
ATCCAAAGTT ACCAGGAAAA AGAAGTGCAG ATGAAACATG AGCTTTCTGT TTTAGAGAAT 
GAACACAACA TTTTACAGGA GAACCTGGAT ACTCTTCAGA AGCAAGTGGT GGAGTTGACC 
GCTTCAGCTT CTCAGAAAGA GTCTGAGCTT CAAAATGAGG TTTGCAAACA AGAGAAACTT 
CAAGAAAAGG CTCAGAAACT TGAGAAGGAT GCAGGTGATC TTCAGGCTAA AATTCTAGAG 
ATTTCCACTC TTGCTTCTGA GAGAGAGGCA CAAATAAGTT CTCTTAAAGA TGAAATAAAC 
AGTCAGCATT TGAAAGCTAA ACAGTCTGAA GATGATCTCC TCAGAGTGTT TGAGGAGAAA 
ATTGAAAACC TTCAAGGACA GCTTGAAATT GCTCGTCTTG ATGTCTCGGA CAAAGACCAG 
CTTCTGCAAA CTTTAAATCA GAAACTGAAA CAAATGGAAG AACTATGCAA GCAAAAGGAG 
ACATGTGTCC TTGAGATGCA TCAGACAAAG GAGGATCTGG AGAAGAAAAC TGTTGAGCTT 
CAAGCTGACA AAAGTCAACA GAATCAGCAG AATTTGGAGA TGTTGAGGCA AGAAAAGGAC 
CATCTGAAAT CTCTAAACCA GACTATCCGA AGAGAGTGTG AAGCGTCCCG GAAGATTCGA 
GCTGAGCTGG AGTTGAACGT TGAGGAACAA ACTGCTTCCA TCCTTGCACT TAAAAAGGCT 
TCCCAACAGT GGGAGGAGCA GAATCAGGAG CTCCTGGAGC AGCTGAAGGC AAAGACAGAA 
GCTGTAGAGC ACTACAAAGC ACAGGTGGAG AAGGCCATGA ATCACTACAA TGGTAAAAAG 
CAGCTGCTGC TGGAGGCTCA AGAGCTGAAC AAAACTCTTG AGCAGTCATT GGAGGTCAGC 
AAGAGAGAAG CTAAAGCTCT GGAAACTGAA CTAACCCTGG CTCGCATGGA GCTCAACCAA 
GCCAATGATA AAGAGAAGAG CCTTGCTGCT AAAGTGAAGA CTTTGGAGGC CCAAGTTGAT 
TTTGCTGACA GGCAACTGCG AGAGAAGAGA AGGATTGCAG ACGATGGTGA GGATTTGAGA 
CAAAAAGAAA ATCTGTACTT CAGAGTCCCA GAGAAGCAGC AAGACACCAG TGCAGACAGC 
CTGGAGTTTG AGCTAAACGA TTCTCTCAAC ACTACCTGTC GTTCTGCAGT ACCGGGTGAG 
TCCAGCACTC CACTGGTCCG TAGTTCTGAG CGTTTGGCTG CTAAACGCCG CGCATTGGGG 
GCTGAATCTC TCGAGACACT ATATTTCACA CCTATGGGTA AACAAGGCTA CAAACGCAAA 
GTGGATCACA ATGATGGTTT TGAACGCAAA CTGGAGTCCA GCATCACTTC TATTGGTGAT 
CTCGTGGTTG ATTCTGCAAA GAAATTGACT GCTTCAGCTC GCCGGCGACG CACTACACAA 
GTTATCAACA TCACCTTGGC TAAGAAGGCA CAAGGAAGCT CTGAAAGCGA GGAGTCGTTT 
TCTAGCCTGC ACTCAGCCCG TTCTCATCCA AATTTGGCAG TCCACCAATC ACGGCCGATC 
TCCATTGACT TCTCAGAGGA TGGTCCTGCC ACAACGTTAT CAAAAGCAGA CACACTTCAA 
AATCTGCCTG GATATCGCAG AAGTACAGTG CACAGCGTTG GCCCACCAAG AGCCACCAGT 
ACTTTTTGTA TTGGAGCAGA GAATGAACCG GAGCATGCTG CCGATGACTG GATGCGCATC 
GCTGAACTGC AGTCCCGAAA CAAAGCCTGT CTGCCTCATC TGAAGAGCAG CTATCCTCTG 
GAGTCCAGGC CCAGTCTAGG ACCATCATTC ACTGTAACGG ATGAGGACCT GCGCTTGGGC 
GACCCGAGTG AGACGATCCG GCGTGCGTCC ATGATGCCGG GTCAGATCCT GGAGTCGCTG 
AACTCTCGCC GTTTCTCCCT CGCTCCTGAA ACGAGTTCGA GCCAGGCTCC TGCACGCTCT 
CATCCCCAGC GAGCCACCAT GCTGCCTTGT CAGATCCAAT CCAGCACTGC TGCTCACCGG 
GCCTCACAGC TCACAAAAAC AAGCTCACGT TCACACACTT CAGAGAATAA ACGCAGCCCG 
CTGGCTCCCA AACGCCCGGC ATCACAACTG CAAGGACCAG ACACTCCTGA GGCAAAGAAG 
TTGGCAAGTT GTTTCCCACG GCCAATGACG CCCAAAGGCC GGTTTGGAAA CAGCCAAAAC 
AGACCTCCAG TCTCTCCGGC TGAGCGAAGA CAGTCTGTGA TGTTTACTGT GGTAAATACT 
CCAAAGAACA GTGCTCGTGG AGACAGCAGG CTTCAGCGCG GCCTCAACAA GCTGCGTAAC 
AGCACCCGCA AATCTCCTGC CGTGGCCAGT CGGGCTTTGC GCTCGGCCGT GAGCGGGAAA 
TCTCCCCTGG ACGTCACTCT GAGGAAATCT CCCCGAAACA AGTCCCCGAA AACATCCAAT 
GCCAAAAAGG AAACACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_009291967.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009290242.1 Danio rerio nuclear mitotic apparatus protein 1 (numa1), transcript variant X3, mRNA.

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CloneID	ODa46286
Clone ID Related Accession (Same CDS sequence)	XM_009291966.3 , XM_009291967.3 , XM_009291967.2 , XM_009291966.2
Accession Version	XM_009291967.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	nuclear mitotic apparatus protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291967.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTTA ATAAGAACAA GGCGGGACCA CTTTTGACAT GGATGAACAG CGTGTTTCCA 
GAGAATCAAA TCTGGGAGTT TGGTCATATG AGAGACGGCT TTCGACTCTT GGAGATCTGC 
TTCAAACTAA AGGGGAGGGA GGACGTTGAA GCTTATAAGA CTTTATCCTT ATACAACAAA 
CTGGAAATCA TCTTTAATGA TTTACATGAT GACTTTCACC TGACTGATAG ACAACGTTTC 
CTCATTTTGG GTAAAATCAG TGAAGGACTT GACCTGGAGC TTCAGCTTGC CAAGGTGGCT 
CTTCTCCTTT GCTATTGCAG TTTTAAAAAG ACTAACCCTG TAGCCCTGAA TTCAAGAACA 
GAGTCGGACA TCACTTTCAT GTTTCGTTTT GTAAGAGATG ATGCCAGTGG TTTGTGTTTA 
GACGAAGGCC TCGATCAATT TCTAGACCAA GACTCTGTTA TGAATCTTGC AAACTCCAGT 
AGCGGGAGCA GCTGCAGTTC TCCATTCTAC ATCGATGAGG AGTCTCCGAT GTTTGGTCGT 
TTCCAGCGTC CTCCCAGAGT TCAGTTTCAA GAGCTTTGCA CAGTGGCTTC CAGCTCAGAC 
AGCTCTCCGA TGCAGGATTT GATGAGCACG CCACACTTCC AGTTGAAGAA ACTCCGCAAA 
GAACTGGCGC ATGAAGGAGA CGTGAGAGAT GAGCTGGAGA AAGAGCTGGC CGATCACATC 
AGCATCATCT CAGAGAAAGA GGGTCTGATC TCCCAGTTAC AGCACCGTGT GGAGCGGATG 
CTGCGGGAAC AAGGAGAGCT GGAGAAAGAC CATAAAGCTG CACTGCTGGA GCTCCAGGAG 
AAGAACGAGA GTCTTCTCCA CAGAGTTCAT GAGGTTTTAA AGCAGTGCCA AGATTTAAAA 
ACAGACAACT CTCAAAAAGA GAAAAAGATT GATGAGCTGA CAGAAGAGAA CGGGACTCTC 
AGTGCTCAGC TTCGTAATGT TTTCTCTCAG CTGGCGAGAG CAGAGCAGGA AGTTGCCAAA 
CTCACAGTGG TTCATGAATC TGCACAGACG GAGTGGAAAA ACAGGAAAGA GTTGCTGGAG 
AAGGAGCTGA ATGAGGCCGT CACACACAAG GAGTGTCTGA GTGAGCAGGT GCTGATACTG 
CAGGGGAAGA TTTCTGTTCT GGAGGATGAA CTGAAGAAAG CTCAGTCTCA GGAGAGAGGA 
GAGGTGCTGG GGCCCATCAT GGAGTGGGAA AAGCTCAAAC AAGAGTTGGC TGATCTCACT 
CTTCAACTCG CTCAGCTTCA GGAATCCATT TCCCACCTCG AGAAGAAAAA GGCAGAAGTT 
GAGGCCTTGT TGGCTGAGGA GAGATGCTCT TTTGAAAAAG AAACCGCAAG ACTCCAGTTG 
GTGGTTTTTG ATCTGGAGAA ATCCATCAAC AGCATCCGTC TGGAGAGAGA AACACTTCAG 
GAGGCCTTAA AGACGCAGAA AGAGATGCTC TCTGCGCAGA TATCAGCACT GGAGAGGGAC 
GTTTCCCGTC TGCAACAGGT TGAAGTGCAG CTGACAGCAG AGATCAAAAT ATCTGCAGAT 
CTCCGCCAAC AGAGAGAGGA GCTGGAAGGA AAGGTAGCAT CTTTAGATAG GATGGTTCAT 
TCTCTGCATG CTGAGATCCA GGGGCTGGAA GTGGAGAGAG CTTCTCAACA GGATGCAATT 
AATTCCCTCA TTAGCGATTT ACAAATCGCT AGGGCTACTG TTCAGGAATA TGAGAAGAAG 
CTGGAAGAGC ATCAAAAAGT GGTAGCAGAA AACAATTCCC TGAAAAATGA AGCATGCACA 
ATGCAGCAGG AGCTGAATAA TCATCTAAAG GCAATCGGAG ACCTTCAGGC GGAAGTAAAT 
GAACTCAGAT TTGAGAAAAC TGAGGAAGAG AACAAGGTTA GTGAGGCTTT GGTTAAAATC 
GAAAGCCTCC AGACTGAGAT TCTGCATCTG TGTGAAAAGA TTTCTCTTAA AGATGAAGAA 
ATCAGAAACC TGACGAAAGA GTATGAGTCT GTAGACAACG AGCTGAAGCT TGTGAAGGAG 
CAAAATGTTG AAATAAATGC AATGATCAAA TCTAATCGCA AGGAGCATGA GGAAACTGTT 
GAAAAACTTC AGCAGGAGCT TCATTGCGCT GCTTCTGCCG CATCAGAAAA ACAAGAGCAA 
ATGCTGGTTT TATCAGCAGA GGTAACATCT CTGAAAGAGC AAATCTGCAG ATATAGTGAA 
AATGAGGCAC AGAAACAACA GGAATTGTCT ATTTTAGAAG CACAACACAA TGTGCTGAAG 
GAAAACCTGA CTTCTCTACA GAACCAGTTG GCTGAGGCGA CAACTTCAGC TTCACAGAAG 
GAATCAGAGT TTATTTTGCT TCAACAGGAG CTTTCCCATC AAGAAACACT AAGAGAACAA 
ACTCAGGAGC TTGAGAAGAT TAAACGTGAG GAGCTTGAGG GGATCGTGAG TGAACTTCAG 
GCCAAAATTA TTGAGGTTTC CTCTATTGCC TCAGAAAGGG AAGCTTGTGT GAGCTCCCTT 
CAATATGAGA TGAAAGATCA GCTATCGAAA GCCAAACAGT GTGAAGCTGA TCTACGCAAT 
GAGATGAAGG AAAAGGTTGG AAATTTGCAG GAACTTCTAG ACGCTGCTAA CCATGGAATT 
GCTGAAAAAG AGCATCACTT AGAGTCGTTG AATCAAAGTC TGAAGCAGAT GGAGCTTCTG 
TACTGTCAAA AGGAGGAAGA AATCATTGCA AGTCAACAAG CAAAGCAGCA ACTGGAGAAT 
AATATTGCTG AGCAGAAACA ACAACTAGAT GAGATTCAGC AAAATCGAGA AATACTAAGG 
AATGAAAGAG ACCATCTGTC TACTCGAGTA TCATCTCTCC AAGAGGAAAT TCATCGTTCC 
CAACAGGATT TTTCTGATTT ACAGACTGAC CACAATGTTT TGAAGGAAAA ACTGGTTAAT 
CTTCAGGATC AAGTAGAGCA GGCGATGATT ACTGCTTCCC AGAAGGAATC TGAACTCCTC 
TTGCTTCAAC AGGAGCTTTC CCATCAAGAG ACCCTGAGAG AAAAAGCTTT GGAGCTTGAG 
GCCATCAAGC GAGAAGAGTT TGAGAAAAAA GTGAAGGAAC TGCAGGACAG AATTCTAGAG 
GTGTCAACTC TTGCCTCTCA AAGGGAAGCC CAGGTAATTT CTCTTGAAAA TGAGATGAGG 
GATCAACAGT TGAGTGCCAA CCAATCTAAA GATAATCTCT GCAGGGAGTG GCAGGAGAAG 
CTTGGCATCT TGAAAGGAGA ATTAGATGTA GTCAGTCGAG ACGCTGCAGA CAAAGACCAT 
CAACTGGAAT CTTTGGATCA AAAGCTTAAA GAGATGGAAA TGGTTGTCCT TCAAAAAGAG 
AAAGATGTAA TGGAGACACA TCAGGCAAAG GAGGACCTGG AGAAGAGAAT TGCTGAGCTT 
GAGGAGTGCA AGCAGAAATT AGAGATTATG AGGAATGAAA GAGATCATCT TTCAACTGAA 
GTAGCATCTC TAAAAGAGGA GATTCATAGC TACCAGGATA CTCAAATGCA GAAACAACAA 
ACAATTTCTG TTTTGGAAGT TGAAAACAAT GCTTTAAAGG AAAACATGGC TGCCCTTGAG 
AAACAATTAG CAGAGGAGAT AACTACAGCT TCACAGAAAA ATTCAGAGCT TCAAAACAAG 
CTTCATCAAC AAGAGAGCCT TCACGAAAAG GCTCAAGAGC TTGAGATTGG CAGGCATGAG 
GAATTTGAGA GAAGAGTGAG AGAACTTCAA GCTAAAGTCC AAGAGGTGTC CACTCTTGCT 
TCTGAGAAGG AGGCTGTTGC TTATTCTCTT CAGGAGGAAT TGACGGGTCA TTTGAAGGCT 
AAAGTGTCCG AAGATGATCT CCGTAGAGTC CTGGAGGAAA AAGTGGAAAC CTTACAGAGA 
GAAATAGAAA CTGCTAGTTG TGATGCCACA AGCAAAGACG GTCTTTTGCA GACTTTGGAT 
CAGAAGCTGA GGCAGATGGA GATGCTTTGC CAACAAAAGG AGGATGCAGT TTTTGAGATC 
CAGAACTCAA AAGAAGACTT GCAGAAAGAG ATGAATGAAC TTGTTTCTAA GAATCAAGAA 
CTGGAGGGCT GCCTGCAACA TTTGGAAATG GTAAAAAAGG AAAAAGACCT CCTGTCAAAT 
GAAGTAACTT CTCTGAAAGA GCAAATAAAT GATCAGAGTT TGAGGGCCAA ACAGTCTGAA 
GCTGATCTTT GCATAGTCTT TGAGGAAAAG ATTGAAACCC TTCAAGGACA ACTGGAATCT 
AGTAGTTGTG ACGTCTCGGA CAAAGAGAAA CATCTGCAAA CTTTGCATCA AAAGGTTTCA 
CAGATGGATC TGTTGTGCCA GCAAAAGGAG AATGCAGTTC TTGAGATGCA GAACGCAAAG 
GAAGACTTGC AGAAGAAGAT TGATGAGCTT GTTTCTGAGA AACAACAACT GGAGGGCTGC 
CTGCAAAATT TGGAAATGGT GAAAAGCGAA AGAGACCTCT TGTCAACTGA AGTAACTTCT 
CTAAAAGATC AACTAAATGA TCAGGATTTG AAAGCCAAAC AGTCCGAAGA TGATCTCCGC 
AAAGTGTTGG AAGAAAAGAT TAAAAACCTC CAGGGACAAC TTGAAACTGC TAGTCGTGAT 
GTCTCCGAAA AAGACCAGCT TCTGCAGACT TTGGATCAAA AGGTTACACA GATGGATCAG 
TTGTGTAAGC AAAAGGAGAA ATATGCTCTG GATTTGCTTC AGGTAAAGGA GAATTTGGAG 
AAGAGGATTG GTGAGATTAC TGTTGAGAAA CAGCAATTGG AAGGATGCCA GCAGAATTTG 
GAAACAGTGA GCAAGGACAA AGACAACCTT TTAAATGAAT TAACCTTTCT TAAAGTTGAA 
ATCCAAAGTT ACCAGGAAAA AGAAGTGCAG ATGAAACATG AGCTTTCTGT TTTAGAGAAT 
GAACACAACA TTTTACAGGA GAACCTGGAT ACTCTTCAGA AGCAAGTGGT GGAGTTGACC 
GCTTCAGCTT CTCAGAAAGA GTCTGAGCTT CAAAATGAGG TTTGCAAACA AGAGAAACTT 
CAAGAAAAGG CTCAGAAACT TGAGAAGGAT GCAGGTGATC TTCAGGCTAA AATTCTAGAG 
ATTTCCACTC TTGCTTCTGA GAGAGAGGCA CAAATAAGTT CTCTTAAAGA TGAAATAAAC 
AGTCAGCATT TGAAAGCTAA ACAGTCTGAA GATGATCTCC TCAGAGTGTT TGAGGAGAAA 
ATTGAAAACC TTCAAGGACA GCTTGAAATT GCTCGTCTTG ATGTCTCGGA CAAAGACCAG 
CTTCTGCAAA CTTTAAATCA GAAACTGAAA CAAATGGAAG AACTATGCAA GCAAAAGGAG 
ACATGTGTCC TTGAGATGCA TCAGACAAAG GAGGATCTGG AGAAGAAAAC TGTTGAGCTT 
CAAGCTGACA AAAGTCAACA GAATCAGCAG AATTTGGAGA TGTTGAGGCA AGAAAAGGAC 
CATCTGAAAT CTCTAAACCA GACTATCCGA AGAGAGTGTG AAGCGTCCCG GAAGATTCGA 
GCTGAGCTGG AGTTGAACGT TGAGGAACAA ACTGCTTCCA TCCTTGCACT TAAAAAGGCT 
TCCCAACAGT GGGAGGAGCA GAATCAGGAG CTCCTGGAGC AGCTGAAGGC AAAGACAGAA 
GCTGTAGAGC ACTACAAAGC ACAGGTGGAG AAGGCCATGA ATCACTACAA TGGTAAAAAG 
CAGCTGCTGC TGGAGGCTCA AGAGCTGAAC AAAACTCTTG AGCAGTCATT GGAGGTCAGC 
AAGAGAGAAG CTAAAGCTCT GGAAACTGAA CTAACCCTGG CTCGCATGGA GCTCAACCAA 
GCCAATGATA AAGAGAAGAG CCTTGCTGCT AAAGTGAAGA CTTTGGAGGC CCAAGTTGAT 
TTTGCTGACA GGCAACTGCG AGAGAAGAGA AGGATTGCAG ACGATGGTGA GGATTTGAGA 
CAAAAAGAAA ATCTGTACTT CAGAGTCCCA GAGAAGCAGC AAGACACCAG TGCAGACAGC 
CTGGAGTTTG AGCTAAACGA TTCTCTCAAC ACTACCTGTC GTTCTGCAGT ACCGGGTGAG 
TCCAGCACTC CACTGGTCCG TAGTTCTGAG CGTTTGGCTG CTAAACGCCG CGCATTGGGG 
GCTGAATCTC TCGAGACACT ATATTTCACA CCTATGGGTA AACAAGGCTA CAAACGCAAA 
GTGGATCACA ATGATGGTTT TGAACGCAAA CTGGAGTCCA GCATCACTTC TATTGGTGAT 
CTCGTGGTTG ATTCTGCAAA GAAATTGACT GCTTCAGCTC GCCGGCGACG CACTACACAA 
GTTATCAACA TCACCTTGGC TAAGAAGGCA CAAGGAAGCT CTGAAAGCGA GGAGTCGTTT 
TCTAGCCTGC ACTCAGCCCG TTCTCATCCA AATTTGGCAG TCCACCAATC ACGGCCGATC 
TCCATTGACT TCTCAGAGGA TGGTCCTGCC ACAACGTTAT CAAAAGCAGA CACACTTCAA 
AATCTGCCTG GATATCGCAG AAGTACAGTG CACAGCGTTG GCCCACCAAG AGCCACCAGT 
ACTTTTTGTA TTGGAGCAGA GAATGAACCG GAGCATGCTG CCGATGACTG GATGCGCATC 
GCTGAACTGC AGTCCCGAAA CAAAGCCTGT CTGCCTCATC TGAAGAGCAG CTATCCTCTG 
GAGTCCAGGC CCAGTCTAGG ACCATCATTC ACTGTAACGG ATGAGGACCT GCGCTTGGGC 
GACCCGAGTG AGACGATCCG GCGTGCGTCC ATGATGCCGG GTCAGATCCT GGAGTCGCTG 
AACTCTCGCC GTTTCTCCCT CGCTCCTGAA ACGAGTTCGA GCCAGGCTCC TGCACGCTCT 
CATCCCCAGC GAGCCACCAT GCTGCCTTGT CAGATCCAAT CCAGCACTGC TGCTCACCGG 
GCCTCACAGC TCACAAAAAC AAGCTCACGT TCACACACTT CAGAGAATAA ACGCAGCCCG 
CTGGCTCCCA AACGCCCGGC ATCACAACTG CAAGGACCAG ACACTCCTGA GGCAAAGAAG 
TTGGCAAGTT GTTTCCCACG GCCAATGACG CCCAAAGGCC GGTTTGGAAA CAGCCAAAAC 
AGACCTCCAG TCTCTCCGGC TGAGCGAAGA CAGTCTGTGA TGTTTACTGT GGTAAATACT 
CCAAAGAACA GTGCTCGTGG AGACAGCAGG CTTCAGCGCG GCCTCAACAA GCTGCGTAAC 
AGCACCCGCA AATCTCCTGC CGTGGCCAGT CGGGCTTTGC GCTCGGCCGT GAGCGGGAAA 
TCTCCCCTGG ACGTCACTCT GAGGAAATCT CCCCGAAACA AGTCCCCGAA AACATCCAAT 
GCCAAAAAGG AAACACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290242.1
CDS	65..7405
Translation	MKFNKNKAGPLLTWMNSVFPENQIWEFGHMRDGFRLLEICFKLKGREDVEAYKTLSLYNKLEIIFNDLHDDFHLTDRQRFLILGKISEGLDLELQLAKVALLLCYCSFKKTNPVALNSRTESDITFMFRFVRDDASGLCLDEGLDQFLDQDSVMNLANSSSGSSCSSPFYIDEESPMFGRFQRPPRVQFQELCTVASSSDSSPMQDLMSTPHFQLKKLRKELAHEGDVRDELEKELADHISIISEKEGLISQLQHRVERMLREQGELEKDHKAALLELQEKNESLLHRVHEVLKQCQDLKTDNSQKEKKIDELTEENGTLSAQLRNVFSQLARAEQEVAKLTVVHESAQTEWKNRKELLEKELNEAVTHKECLSEQVLILQGKISVLEDELKKAQSQERGEVLGPIMEWEKLKQELADLTLQLAQLQESISHLEKKKAEVEALLAEERCSFEKETARLQLVVFDLEKSINSIRLERETLQEALKTQKEMLSAQISALERDVSRLQQVEVQLTAEIKISADLRQQREELEGKVASLDRMVHSLHAEIQGLEVERASQQDAINSLISDLQIARATVQEYEKKLEEHQKVVAENNSLKNEACTMQQELNNHLKAIGDLQAEVNELRFEKTEEENKVSEALVKIESLQTEILHLCEKISLKDEEIRNLTKEYESVDNELKLVKEQNVEINAMIKSNRKEHEETVEKLQQELHCAASAASEKQEQMLVLSAEVTSLKEQICRYSENEAQKQQELSILEAQHNVLKENLTSLQNQLAEATTSASQKESEFILLQQELSHQETLREQTQELEKIKREELEGIVSELQAKIIEVSSIASEREACVSSLQYEMKDQLSKAKQCEADLRNEMKEKVGNLQELLDAANHGIAEKEHHLESLNQSLKQMELLYCQKEEEIIASQQAKQQLENNIAEQKQQLDEIQQNREILRNERDHLSTRVSSLQEEIHRSQQDFSDLQTDHNVLKEKLVNLQDQVEQAMITASQKESELLLLQQELSHQETLREKALELEAIKREEFEKKVKELQDRILEVSTLASQREAQVISLENEMRDQQLSANQSKDNLCREWQEKLGILKGELDVVSRDAADKDHQLESLDQKLKEMEMVVLQKEKDVMETHQAKEDLEKRIAELEECKQKLEIMRNERDHLSTEVASLKEEIHSYQDTQMQKQQTISVLEVENNALKENMAALEKQLAEEITTASQKNSELQNKLHQQESLHEKAQELEIGRHEEFERRVRELQAKVQEVSTLASEKEAVAYSLQEELTGHLKAKVSEDDLRRVLEEKVETLQREIETASCDATSKDGLLQTLDQKLRQMEMLCQQKEDAVFEIQNSKEDLQKEMNELVSKNQELEGCLQHLEMVKKEKDLLSNEVTSLKEQINDQSLRAKQSEADLCIVFEEKIETLQGQLESSSCDVSDKEKHLQTLHQKVSQMDLLCQQKENAVLEMQNAKEDLQKKIDELVSEKQQLEGCLQNLEMVKSERDLLSTEVTSLKDQLNDQDLKAKQSEDDLRKVLEEKIKNLQGQLETASRDVSEKDQLLQTLDQKVTQMDQLCKQKEKYALDLLQVKENLEKRIGEITVEKQQLEGCQQNLETVSKDKDNLLNELTFLKVEIQSYQEKEVQMKHELSVLENEHNILQENLDTLQKQVVELTASASQKESELQNEVCKQEKLQEKAQKLEKDAGDLQAKILEISTLASEREAQISSLKDEINSQHLKAKQSEDDLLRVFEEKIENLQGQLEIARLDVSDKDQLLQTLNQKLKQMEELCKQKETCVLEMHQTKEDLEKKTVELQADKSQQNQQNLEMLRQEKDHLKSLNQTIRRECEASRKIRAELELNVEEQTASILALKKASQQWEEQNQELLEQLKAKTEAVEHYKAQVEKAMNHYNGKKQLLLEAQELNKTLEQSLEVSKREAKALETELTLARMELNQANDKEKSLAAKVKTLEAQVDFADRQLREKRRIADDGEDLRQKENLYFRVPEKQQDTSADSLEFELNDSLNTTCRSAVPGESSTPLVRSSERLAAKRRALGAESLETLYFTPMGKQGYKRKVDHNDGFERKLESSITSIGDLVVDSAKKLTASARRRRTTQVINITLAKKAQGSSESEESFSSLHSARSHPNLAVHQSRPISIDFSEDGPATTLSKADTLQNLPGYRRSTVHSVGPPRATSTFCIGAENEPEHAADDWMRIAELQSRNKACLPHLKSSYPLESRPSLGPSFTVTDEDLRLGDPSETIRRASMMPGQILESLNSRRFSLAPETSSSQAPARSHPQRATMLPCQIQSSTAAHRASQLTKTSSRSHTSENKRSPLAPKRPASQLQGPDTPEAKKLASCFPRPMTPKGRFGNSQNRPPVSPAERRQSVMFTVVNTPKNSARGDSRLQRGLNKLRNSTRKSPAVASRALRSAVSGKSPLDVTLRKSPRNKSPKTSNAKKETQ

Target ORF information:

RefSeq Version	XM_009291967.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nuclear mitotic apparatus protein 1 (numa1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291967.2

ORF Insert Sequence:

ATGAAGTTTA ATAAGAACAA GGCGGGACCA CTTTTGACAT GGATGAACAG CGTGTTTCCA 
GAGAATCAAA TCTGGGAGTT TGGTCATATG AGAGACGGCT TTCGACTCTT GGAGATCTGC 
TTCAAACTAA AGGGGAGGGA GGACGTTGAA GCTTATAAGA CTTTATCCTT ATACAACAAA 
CTGGAAATCA TCTTTAATGA TTTACATGAT GACTTTCACC TGACTGATAG ACAACGTTTC 
CTCATTTTGG GTAAAATCAG TGAAGGACTT GACCTGGAGC TTCAGCTTGC CAAGGTGGCT 
CTTCTCCTTT GCTATTGCAG TTTTAAAAAG ACTAACCCTG TAGCCCTGAA TTCAAGAACA 
GAGTCGGACA TCACTTTCAT GTTTCGTTTT GTAAGAGATG ATGCCAGTGG TTTGTGTTTA 
GACGAAGGCC TCGATCAATT TCTAGACCAA GACTCTGTTA TGAATCTTGC AAACTCCAGT 
AGCGGGAGCA GCTGCAGTTC TCCATTCTAC ATCGATGAGG AGTCTCCGAT GTTTGGTCGT 
TTCCAGCGTC CTCCCAGAGT TCAGTTTCAA GAGCTTTGCA CAGTGGCTTC CAGCTCAGAC 
AGCTCTCCGA TGCAGGATTT GATGAGCACG CCACACTTCC AGTTGAAGAA ACTCCGCAAA 
GAACTGGCGC ATGAAGGAGA CGTGAGAGAT GAGCTGGAGA AAGAGCTGGC CGATCACATC 
AGCATCATCT CAGAGAAAGA GGGTCTGATC TCCCAGTTAC AGCACCGTGT GGAGCGGATG 
CTGCGGGAAC AAGGAGAGCT GGAGAAAGAC CATAAAGCTG CACTGCTGGA GCTCCAGGAG 
AAGAACGAGA GTCTTCTCCA CAGAGTTCAT GAGGTTTTAA AGCAGTGCCA AGATTTAAAA 
ACAGACAACT CTCAAAAAGA GAAAAAGATT GATGAGCTGA CAGAAGAGAA CGGGACTCTC 
AGTGCTCAGC TTCGTAATGT TTTCTCTCAG CTGGCGAGAG CAGAGCAGGA AGTTGCCAAA 
CTCACAGTGG TTCATGAATC TGCACAGACG GAGTGGAAAA ACAGGAAAGA GTTGCTGGAG 
AAGGAGCTGA ATGAGGCCGT CACACACAAG GAGTGTCTGA GTGAGCAGGT GCTGATACTG 
CAGGGGAAGA TTTCTGTTCT GGAGGATGAA CTGAAGAAAG CTCAGTCTCA GGAGAGAGGA 
GAGGTGCTGG GGCCCATCAT GGAGTGGGAA AAGCTCAAAC AAGAGTTGGC TGATCTCACT 
CTTCAACTCG CTCAGCTTCA GGAATCCATT TCCCACCTCG AGAAGAAAAA GGCAGAAGTT 
GAGGCCTTGT TGGCTGAGGA GAGATGCTCT TTTGAAAAAG AAACCGCAAG ACTCCAGTTG 
GTGGTTTTTG ATCTGGAGAA ATCCATCAAC AGCATCCGTC TGGAGAGAGA AACACTTCAG 
GAGGCCTTAA AGACGCAGAA AGAGATGCTC TCTGCGCAGA TATCAGCACT GGAGAGGGAC 
GTTTCCCGTC TGCAACAGGT TGAAGTGCAG CTGACAGCAG AGATCAAAAT ATCTGCAGAT 
CTCCGCCAAC AGAGAGAGGA GCTGGAAGGA AAGGTAGCAT CTTTAGATAG GATGGTTCAT 
TCTCTGCATG CTGAGATCCA GGGGCTGGAA GTGGAGAGAG CTTCTCAACA GGATGCAATT 
AATTCCCTCA TTAGCGATTT ACAAATCGCT AGGGCTACTG TTCAGGAATA TGAGAAGAAG 
CTGGAAGAGC ATCAAAAAGT GGTAGCAGAA AACAATTCCC TGAAAAATGA AGCATGCACA 
ATGCAGCAGG AGCTGAATAA TCATCTAAAG GCAATCGGAG ACCTTCAGGC GGAAGTAAAT 
GAACTCAGAT TTGAGAAAAC TGAGGAAGAG AACAAGGTTA GTGAGGCTTT GGTTAAAATC 
GAAAGCCTCC AGACTGAGAT TCTGCATCTG TGTGAAAAGA TTTCTCTTAA AGATGAAGAA 
ATCAGAAACC TGACGAAAGA GTATGAGTCT GTAGACAACG AGCTGAAGCT TGTGAAGGAG 
CAAAATGTTG AAATAAATGC AATGATCAAA TCTAATCGCA AGGAGCATGA GGAAACTGTT 
GAAAAACTTC AGCAGGAGCT TCATTGCGCT GCTTCTGCCG CATCAGAAAA ACAAGAGCAA 
ATGCTGGTTT TATCAGCAGA GGTAACATCT CTGAAAGAGC AAATCTGCAG ATATAGTGAA 
AATGAGGCAC AGAAACAACA GGAATTGTCT ATTTTAGAAG CACAACACAA TGTGCTGAAG 
GAAAACCTGA CTTCTCTACA GAACCAGTTG GCTGAGGCGA CAACTTCAGC TTCACAGAAG 
GAATCAGAGT TTATTTTGCT TCAACAGGAG CTTTCCCATC AAGAAACACT AAGAGAACAA 
ACTCAGGAGC TTGAGAAGAT TAAACGTGAG GAGCTTGAGG GGATCGTGAG TGAACTTCAG 
GCCAAAATTA TTGAGGTTTC CTCTATTGCC TCAGAAAGGG AAGCTTGTGT GAGCTCCCTT 
CAATATGAGA TGAAAGATCA GCTATCGAAA GCCAAACAGT GTGAAGCTGA TCTACGCAAT 
GAGATGAAGG AAAAGGTTGG AAATTTGCAG GAACTTCTAG ACGCTGCTAA CCATGGAATT 
GCTGAAAAAG AGCATCACTT AGAGTCGTTG AATCAAAGTC TGAAGCAGAT GGAGCTTCTG 
TACTGTCAAA AGGAGGAAGA AATCATTGCA AGTCAACAAG CAAAGCAGCA ACTGGAGAAT 
AATATTGCTG AGCAGAAACA ACAACTAGAT GAGATTCAGC AAAATCGAGA AATACTAAGG 
AATGAAAGAG ACCATCTGTC TACTCGAGTA TCATCTCTCC AAGAGGAAAT TCATCGTTCC 
CAACAGGATT TTTCTGATTT ACAGACTGAC CACAATGTTT TGAAGGAAAA ACTGGTTAAT 
CTTCAGGATC AAGTAGAGCA GGCGATGATT ACTGCTTCCC AGAAGGAATC TGAACTCCTC 
TTGCTTCAAC AGGAGCTTTC CCATCAAGAG ACCCTGAGAG AAAAAGCTTT GGAGCTTGAG 
GCCATCAAGC GAGAAGAGTT TGAGAAAAAA GTGAAGGAAC TGCAGGACAG AATTCTAGAG 
GTGTCAACTC TTGCCTCTCA AAGGGAAGCC CAGGTAATTT CTCTTGAAAA TGAGATGAGG 
GATCAACAGT TGAGTGCCAA CCAATCTAAA GATAATCTCT GCAGGGAGTG GCAGGAGAAG 
CTTGGCATCT TGAAAGGAGA ATTAGATGTA GTCAGTCGAG ACGCTGCAGA CAAAGACCAT 
CAACTGGAAT CTTTGGATCA AAAGCTTAAA GAGATGGAAA TGGTTGTCCT TCAAAAAGAG 
AAAGATGTAA TGGAGACACA TCAGGCAAAG GAGGACCTGG AGAAGAGAAT TGCTGAGCTT 
GAGGAGTGCA AGCAGAAATT AGAGATTATG AGGAATGAAA GAGATCATCT TTCAACTGAA 
GTAGCATCTC TAAAAGAGGA GATTCATAGC TACCAGGATA CTCAAATGCA GAAACAACAA 
ACAATTTCTG TTTTGGAAGT TGAAAACAAT GCTTTAAAGG AAAACATGGC TGCCCTTGAG 
AAACAATTAG CAGAGGAGAT AACTACAGCT TCACAGAAAA ATTCAGAGCT TCAAAACAAG 
CTTCATCAAC AAGAGAGCCT TCACGAAAAG GCTCAAGAGC TTGAGATTGG CAGGCATGAG 
GAATTTGAGA GAAGAGTGAG AGAACTTCAA GCTAAAGTCC AAGAGGTGTC CACTCTTGCT 
TCTGAGAAGG AGGCTGTTGC TTATTCTCTT CAGGAGGAAT TGACGGGTCA TTTGAAGGCT 
AAAGTGTCCG AAGATGATCT CCGTAGAGTC CTGGAGGAAA AAGTGGAAAC CTTACAGAGA 
GAAATAGAAA CTGCTAGTTG TGATGCCACA AGCAAAGACG GTCTTTTGCA GACTTTGGAT 
CAGAAGCTGA GGCAGATGGA GATGCTTTGC CAACAAAAGG AGGATGCAGT TTTTGAGATC 
CAGAACTCAA AAGAAGACTT GCAGAAAGAG ATGAATGAAC TTGTTTCTAA GAATCAAGAA 
CTGGAGGGCT GCCTGCAACA TTTGGAAATG GTAAAAAAGG AAAAAGACCT CCTGTCAAAT 
GAAGTAACTT CTCTGAAAGA GCAAATAAAT GATCAGAGTT TGAGGGCCAA ACAGTCTGAA 
GCTGATCTTT GCATAGTCTT TGAGGAAAAG ATTGAAACCC TTCAAGGACA ACTGGAATCT 
AGTAGTTGTG ACGTCTCGGA CAAAGAGAAA CATCTGCAAA CTTTGCATCA AAAGGTTTCA 
CAGATGGATC TGTTGTGCCA GCAAAAGGAG AATGCAGTTC TTGAGATGCA GAACGCAAAG 
GAAGACTTGC AGAAGAAGAT TGATGAGCTT GTTTCTGAGA AACAACAACT GGAGGGCTGC 
CTGCAAAATT TGGAAATGGT GAAAAGCGAA AGAGACCTCT TGTCAACTGA AGTAACTTCT 
CTAAAAGATC AACTAAATGA TCAGGATTTG AAAGCCAAAC AGTCCGAAGA TGATCTCCGC 
AAAGTGTTGG AAGAAAAGAT TAAAAACCTC CAGGGACAAC TTGAAACTGC TAGTCGTGAT 
GTCTCCGAAA AAGACCAGCT TCTGCAGACT TTGGATCAAA AGGTTACACA GATGGATCAG 
TTGTGTAAGC AAAAGGAGAA ATATGCTCTG GATTTGCTTC AGGTAAAGGA GAATTTGGAG 
AAGAGGATTG GTGAGATTAC TGTTGAGAAA CAGCAATTGG AAGGATGCCA GCAGAATTTG 
GAAACAGTGA GCAAGGACAA AGACAACCTT TTAAATGAAT TAACCTTTCT TAAAGTTGAA 
ATCCAAAGTT ACCAGGAAAA AGAAGTGCAG ATGAAACATG AGCTTTCTGT TTTAGAGAAT 
GAACACAACA TTTTACAGGA GAACCTGGAT ACTCTTCAGA AGCAAGTGGT GGAGTTGACC 
GCTTCAGCTT CTCAGAAAGA GTCTGAGCTT CAAAATGAGG TTTGCAAACA AGAGAAACTT 
CAAGAAAAGG CTCAGAAACT TGAGAAGGAT GCAGGTGATC TTCAGGCTAA AATTCTAGAG 
ATTTCCACTC TTGCTTCTGA GAGAGAGGCA CAAATAAGTT CTCTTAAAGA TGAAATAAAC 
AGTCAGCATT TGAAAGCTAA ACAGTCTGAA GATGATCTCC TCAGAGTGTT TGAGGAGAAA 
ATTGAAAACC TTCAAGGACA GCTTGAAATT GCTCGTCTTG ATGTCTCGGA CAAAGACCAG 
CTTCTGCAAA CTTTAAATCA GAAACTGAAA CAAATGGAAG AACTATGCAA GCAAAAGGAG 
ACATGTGTCC TTGAGATGCA TCAGACAAAG GAGGATCTGG AGAAGAAAAC TGTTGAGCTT 
CAAGCTGACA AAAGTCAACA GAATCAGCAG AATTTGGAGA TGTTGAGGCA AGAAAAGGAC 
CATCTGAAAT CTCTAAACCA GACTATCCGA AGAGAGTGTG AAGCGTCCCG GAAGATTCGA 
GCTGAGCTGG AGTTGAACGT TGAGGAACAA ACTGCTTCCA TCCTTGCACT TAAAAAGGCT 
TCCCAACAGT GGGAGGAGCA GAATCAGGAG CTCCTGGAGC AGCTGAAGGC AAAGACAGAA 
GCTGTAGAGC ACTACAAAGC ACAGGTGGAG AAGGCCATGA ATCACTACAA TGGTAAAAAG 
CAGCTGCTGC TGGAGGCTCA AGAGCTGAAC AAAACTCTTG AGCAGTCATT GGAGGTCAGC 
AAGAGAGAAG CTAAAGCTCT GGAAACTGAA CTAACCCTGG CTCGCATGGA GCTCAACCAA 
GCCAATGATA AAGAGAAGAG CCTTGCTGCT AAAGTGAAGA CTTTGGAGGC CCAAGTTGAT 
TTTGCTGACA GGCAACTGCG AGAGAAGAGA AGGATTGCAG ACGATGGTGA GGATTTGAGA 
CAAAAAGAAA ATCTGTACTT CAGAGTCCCA GAGAAGCAGC AAGACACCAG TGCAGACAGC 
CTGGAGTTTG AGCTAAACGA TTCTCTCAAC ACTACCTGTC GTTCTGCAGT ACCGGGTGAG 
TCCAGCACTC CACTGGTCCG TAGTTCTGAG CGTTTGGCTG CTAAACGCCG CGCATTGGGG 
GCTGAATCTC TCGAGACACT ATATTTCACA CCTATGGGTA AACAAGGCTA CAAACGCAAA 
GTGGATCACA ATGATGGTTT TGAACGCAAA CTGGAGTCCA GCATCACTTC TATTGGTGAT 
CTCGTGGTTG ATTCTGCAAA GAAATTGACT GCTTCAGCTC GCCGGCGACG CACTACACAA 
GTTATCAACA TCACCTTGGC TAAGAAGGCA CAAGGAAGCT CTGAAAGCGA GGAGTCGTTT 
TCTAGCCTGC ACTCAGCCCG TTCTCATCCA AATTTGGCAG TCCACCAATC ACGGCCGATC 
TCCATTGACT TCTCAGAGGA TGGTCCTGCC ACAACGTTAT CAAAAGCAGA CACACTTCAA 
AATCTGCCTG GATATCGCAG AAGTACAGTG CACAGCGTTG GCCCACCAAG AGCCACCAGT 
ACTTTTTGTA TTGGAGCAGA GAATGAACCG GAGCATGCTG CCGATGACTG GATGCGCATC 
GCTGAACTGC AGTCCCGAAA CAAAGCCTGT CTGCCTCATC TGAAGAGCAG CTATCCTCTG 
GAGTCCAGGC CCAGTCTAGG ACCATCATTC ACTGTAACGG ATGAGGACCT GCGCTTGGGC 
GACCCGAGTG AGACGATCCG GCGTGCGTCC ATGATGCCGG GTCAGATCCT GGAGTCGCTG 
AACTCTCGCC GTTTCTCCCT CGCTCCTGAA ACGAGTTCGA GCCAGGCTCC TGCACGCTCT 
CATCCCCAGC GAGCCACCAT GCTGCCTTGT CAGATCCAAT CCAGCACTGC TGCTCACCGG 
GCCTCACAGC TCACAAAAAC AAGCTCACGT TCACACACTT CAGAGAATAA ACGCAGCCCG 
CTGGCTCCCA AACGCCCGGC ATCACAACTG CAAGGACCAG ACACTCCTGA GGCAAAGAAG 
TTGGCAAGTT GTTTCCCACG GCCAATGACG CCCAAAGGCC GGTTTGGAAA CAGCCAAAAC 
AGACCTCCAG TCTCTCCGGC TGAGCGAAGA CAGTCTGTGA TGTTTACTGT GGTAAATACT 
CCAAAGAACA GTGCTCGTGG AGACAGCAGG CTTCAGCGCG GCCTCAACAA GCTGCGTAAC 
AGCACCCGCA AATCTCCTGC CGTGGCCAGT CGGGCTTTGC GCTCGGCCGT GAGCGGGAAA 
TCTCCCCTGG ACGTCACTCT GAGGAAATCT CCCCGAAACA AGTCCCCGAA AACATCCAAT 
GCCAAAAAGG AAACACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_009291966.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009290241.1 Danio rerio nuclear mitotic apparatus protein 1 (numa1), transcript variant X2, mRNA.

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CloneID	ODa46286
Clone ID Related Accession (Same CDS sequence)	XM_009291966.3 , XM_009291967.3 , XM_009291967.2 , XM_009291966.2
Accession Version	XM_009291966.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	nuclear mitotic apparatus protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTTA ATAAGAACAA GGCGGGACCA CTTTTGACAT GGATGAACAG CGTGTTTCCA 
GAGAATCAAA TCTGGGAGTT TGGTCATATG AGAGACGGCT TTCGACTCTT GGAGATCTGC 
TTCAAACTAA AGGGGAGGGA GGACGTTGAA GCTTATAAGA CTTTATCCTT ATACAACAAA 
CTGGAAATCA TCTTTAATGA TTTACATGAT GACTTTCACC TGACTGATAG ACAACGTTTC 
CTCATTTTGG GTAAAATCAG TGAAGGACTT GACCTGGAGC TTCAGCTTGC CAAGGTGGCT 
CTTCTCCTTT GCTATTGCAG TTTTAAAAAG ACTAACCCTG TAGCCCTGAA TTCAAGAACA 
GAGTCGGACA TCACTTTCAT GTTTCGTTTT GTAAGAGATG ATGCCAGTGG TTTGTGTTTA 
GACGAAGGCC TCGATCAATT TCTAGACCAA GACTCTGTTA TGAATCTTGC AAACTCCAGT 
AGCGGGAGCA GCTGCAGTTC TCCATTCTAC ATCGATGAGG AGTCTCCGAT GTTTGGTCGT 
TTCCAGCGTC CTCCCAGAGT TCAGTTTCAA GAGCTTTGCA CAGTGGCTTC CAGCTCAGAC 
AGCTCTCCGA TGCAGGATTT GATGAGCACG CCACACTTCC AGTTGAAGAA ACTCCGCAAA 
GAACTGGCGC ATGAAGGAGA CGTGAGAGAT GAGCTGGAGA AAGAGCTGGC CGATCACATC 
AGCATCATCT CAGAGAAAGA GGGTCTGATC TCCCAGTTAC AGCACCGTGT GGAGCGGATG 
CTGCGGGAAC AAGGAGAGCT GGAGAAAGAC CATAAAGCTG CACTGCTGGA GCTCCAGGAG 
AAGAACGAGA GTCTTCTCCA CAGAGTTCAT GAGGTTTTAA AGCAGTGCCA AGATTTAAAA 
ACAGACAACT CTCAAAAAGA GAAAAAGATT GATGAGCTGA CAGAAGAGAA CGGGACTCTC 
AGTGCTCAGC TTCGTAATGT TTTCTCTCAG CTGGCGAGAG CAGAGCAGGA AGTTGCCAAA 
CTCACAGTGG TTCATGAATC TGCACAGACG GAGTGGAAAA ACAGGAAAGA GTTGCTGGAG 
AAGGAGCTGA ATGAGGCCGT CACACACAAG GAGTGTCTGA GTGAGCAGGT GCTGATACTG 
CAGGGGAAGA TTTCTGTTCT GGAGGATGAA CTGAAGAAAG CTCAGTCTCA GGAGAGAGGA 
GAGGTGCTGG GGCCCATCAT GGAGTGGGAA AAGCTCAAAC AAGAGTTGGC TGATCTCACT 
CTTCAACTCG CTCAGCTTCA GGAATCCATT TCCCACCTCG AGAAGAAAAA GGCAGAAGTT 
GAGGCCTTGT TGGCTGAGGA GAGATGCTCT TTTGAAAAAG AAACCGCAAG ACTCCAGTTG 
GTGGTTTTTG ATCTGGAGAA ATCCATCAAC AGCATCCGTC TGGAGAGAGA AACACTTCAG 
GAGGCCTTAA AGACGCAGAA AGAGATGCTC TCTGCGCAGA TATCAGCACT GGAGAGGGAC 
GTTTCCCGTC TGCAACAGGT TGAAGTGCAG CTGACAGCAG AGATCAAAAT ATCTGCAGAT 
CTCCGCCAAC AGAGAGAGGA GCTGGAAGGA AAGGTAGCAT CTTTAGATAG GATGGTTCAT 
TCTCTGCATG CTGAGATCCA GGGGCTGGAA GTGGAGAGAG CTTCTCAACA GGATGCAATT 
AATTCCCTCA TTAGCGATTT ACAAATCGCT AGGGCTACTG TTCAGGAATA TGAGAAGAAG 
CTGGAAGAGC ATCAAAAAGT GGTAGCAGAA AACAATTCCC TGAAAAATGA AGCATGCACA 
ATGCAGCAGG AGCTGAATAA TCATCTAAAG GCAATCGGAG ACCTTCAGGC GGAAGTAAAT 
GAACTCAGAT TTGAGAAAAC TGAGGAAGAG AACAAGGTTA GTGAGGCTTT GGTTAAAATC 
GAAAGCCTCC AGACTGAGAT TCTGCATCTG TGTGAAAAGA TTTCTCTTAA AGATGAAGAA 
ATCAGAAACC TGACGAAAGA GTATGAGTCT GTAGACAACG AGCTGAAGCT TGTGAAGGAG 
CAAAATGTTG AAATAAATGC AATGATCAAA TCTAATCGCA AGGAGCATGA GGAAACTGTT 
GAAAAACTTC AGCAGGAGCT TCATTGCGCT GCTTCTGCCG CATCAGAAAA ACAAGAGCAA 
ATGCTGGTTT TATCAGCAGA GGTAACATCT CTGAAAGAGC AAATCTGCAG ATATAGTGAA 
AATGAGGCAC AGAAACAACA GGAATTGTCT ATTTTAGAAG CACAACACAA TGTGCTGAAG 
GAAAACCTGA CTTCTCTACA GAACCAGTTG GCTGAGGCGA CAACTTCAGC TTCACAGAAG 
GAATCAGAGT TTATTTTGCT TCAACAGGAG CTTTCCCATC AAGAAACACT AAGAGAACAA 
ACTCAGGAGC TTGAGAAGAT TAAACGTGAG GAGCTTGAGG GGATCGTGAG TGAACTTCAG 
GCCAAAATTA TTGAGGTTTC CTCTATTGCC TCAGAAAGGG AAGCTTGTGT GAGCTCCCTT 
CAATATGAGA TGAAAGATCA GCTATCGAAA GCCAAACAGT GTGAAGCTGA TCTACGCAAT 
GAGATGAAGG AAAAGGTTGG AAATTTGCAG GAACTTCTAG ACGCTGCTAA CCATGGAATT 
GCTGAAAAAG AGCATCACTT AGAGTCGTTG AATCAAAGTC TGAAGCAGAT GGAGCTTCTG 
TACTGTCAAA AGGAGGAAGA AATCATTGCA AGTCAACAAG CAAAGCAGCA ACTGGAGAAT 
AATATTGCTG AGCAGAAACA ACAACTAGAT GAGATTCAGC AAAATCGAGA AATACTAAGG 
AATGAAAGAG ACCATCTGTC TACTCGAGTA TCATCTCTCC AAGAGGAAAT TCATCGTTCC 
CAACAGGATT TTTCTGATTT ACAGACTGAC CACAATGTTT TGAAGGAAAA ACTGGTTAAT 
CTTCAGGATC AAGTAGAGCA GGCGATGATT ACTGCTTCCC AGAAGGAATC TGAACTCCTC 
TTGCTTCAAC AGGAGCTTTC CCATCAAGAG ACCCTGAGAG AAAAAGCTTT GGAGCTTGAG 
GCCATCAAGC GAGAAGAGTT TGAGAAAAAA GTGAAGGAAC TGCAGGACAG AATTCTAGAG 
GTGTCAACTC TTGCCTCTCA AAGGGAAGCC CAGGTAATTT CTCTTGAAAA TGAGATGAGG 
GATCAACAGT TGAGTGCCAA CCAATCTAAA GATAATCTCT GCAGGGAGTG GCAGGAGAAG 
CTTGGCATCT TGAAAGGAGA ATTAGATGTA GTCAGTCGAG ACGCTGCAGA CAAAGACCAT 
CAACTGGAAT CTTTGGATCA AAAGCTTAAA GAGATGGAAA TGGTTGTCCT TCAAAAAGAG 
AAAGATGTAA TGGAGACACA TCAGGCAAAG GAGGACCTGG AGAAGAGAAT TGCTGAGCTT 
GAGGAGTGCA AGCAGAAATT AGAGATTATG AGGAATGAAA GAGATCATCT TTCAACTGAA 
GTAGCATCTC TAAAAGAGGA GATTCATAGC TACCAGGATA CTCAAATGCA GAAACAACAA 
ACAATTTCTG TTTTGGAAGT TGAAAACAAT GCTTTAAAGG AAAACATGGC TGCCCTTGAG 
AAACAATTAG CAGAGGAGAT AACTACAGCT TCACAGAAAA ATTCAGAGCT TCAAAACAAG 
CTTCATCAAC AAGAGAGCCT TCACGAAAAG GCTCAAGAGC TTGAGATTGG CAGGCATGAG 
GAATTTGAGA GAAGAGTGAG AGAACTTCAA GCTAAAGTCC AAGAGGTGTC CACTCTTGCT 
TCTGAGAAGG AGGCTGTTGC TTATTCTCTT CAGGAGGAAT TGACGGGTCA TTTGAAGGCT 
AAAGTGTCCG AAGATGATCT CCGTAGAGTC CTGGAGGAAA AAGTGGAAAC CTTACAGAGA 
GAAATAGAAA CTGCTAGTTG TGATGCCACA AGCAAAGACG GTCTTTTGCA GACTTTGGAT 
CAGAAGCTGA GGCAGATGGA GATGCTTTGC CAACAAAAGG AGGATGCAGT TTTTGAGATC 
CAGAACTCAA AAGAAGACTT GCAGAAAGAG ATGAATGAAC TTGTTTCTAA GAATCAAGAA 
CTGGAGGGCT GCCTGCAACA TTTGGAAATG GTAAAAAAGG AAAAAGACCT CCTGTCAAAT 
GAAGTAACTT CTCTGAAAGA GCAAATAAAT GATCAGAGTT TGAGGGCCAA ACAGTCTGAA 
GCTGATCTTT GCATAGTCTT TGAGGAAAAG ATTGAAACCC TTCAAGGACA ACTGGAATCT 
AGTAGTTGTG ACGTCTCGGA CAAAGAGAAA CATCTGCAAA CTTTGCATCA AAAGGTTTCA 
CAGATGGATC TGTTGTGCCA GCAAAAGGAG AATGCAGTTC TTGAGATGCA GAACGCAAAG 
GAAGACTTGC AGAAGAAGAT TGATGAGCTT GTTTCTGAGA AACAACAACT GGAGGGCTGC 
CTGCAAAATT TGGAAATGGT GAAAAGCGAA AGAGACCTCT TGTCAACTGA AGTAACTTCT 
CTAAAAGATC AACTAAATGA TCAGGATTTG AAAGCCAAAC AGTCCGAAGA TGATCTCCGC 
AAAGTGTTGG AAGAAAAGAT TAAAAACCTC CAGGGACAAC TTGAAACTGC TAGTCGTGAT 
GTCTCCGAAA AAGACCAGCT TCTGCAGACT TTGGATCAAA AGGTTACACA GATGGATCAG 
TTGTGTAAGC AAAAGGAGAA ATATGCTCTG GATTTGCTTC AGGTAAAGGA GAATTTGGAG 
AAGAGGATTG GTGAGATTAC TGTTGAGAAA CAGCAATTGG AAGGATGCCA GCAGAATTTG 
GAAACAGTGA GCAAGGACAA AGACAACCTT TTAAATGAAT TAACCTTTCT TAAAGTTGAA 
ATCCAAAGTT ACCAGGAAAA AGAAGTGCAG ATGAAACATG AGCTTTCTGT TTTAGAGAAT 
GAACACAACA TTTTACAGGA GAACCTGGAT ACTCTTCAGA AGCAAGTGGT GGAGTTGACC 
GCTTCAGCTT CTCAGAAAGA GTCTGAGCTT CAAAATGAGG TTTGCAAACA AGAGAAACTT 
CAAGAAAAGG CTCAGAAACT TGAGAAGGAT GCAGGTGATC TTCAGGCTAA AATTCTAGAG 
ATTTCCACTC TTGCTTCTGA GAGAGAGGCA CAAATAAGTT CTCTTAAAGA TGAAATAAAC 
AGTCAGCATT TGAAAGCTAA ACAGTCTGAA GATGATCTCC TCAGAGTGTT TGAGGAGAAA 
ATTGAAAACC TTCAAGGACA GCTTGAAATT GCTCGTCTTG ATGTCTCGGA CAAAGACCAG 
CTTCTGCAAA CTTTAAATCA GAAACTGAAA CAAATGGAAG AACTATGCAA GCAAAAGGAG 
ACATGTGTCC TTGAGATGCA TCAGACAAAG GAGGATCTGG AGAAGAAAAC TGTTGAGCTT 
CAAGCTGACA AAAGTCAACA GAATCAGCAG AATTTGGAGA TGTTGAGGCA AGAAAAGGAC 
CATCTGAAAT CTCTAAACCA GACTATCCGA AGAGAGTGTG AAGCGTCCCG GAAGATTCGA 
GCTGAGCTGG AGTTGAACGT TGAGGAACAA ACTGCTTCCA TCCTTGCACT TAAAAAGGCT 
TCCCAACAGT GGGAGGAGCA GAATCAGGAG CTCCTGGAGC AGCTGAAGGC AAAGACAGAA 
GCTGTAGAGC ACTACAAAGC ACAGGTGGAG AAGGCCATGA ATCACTACAA TGGTAAAAAG 
CAGCTGCTGC TGGAGGCTCA AGAGCTGAAC AAAACTCTTG AGCAGTCATT GGAGGTCAGC 
AAGAGAGAAG CTAAAGCTCT GGAAACTGAA CTAACCCTGG CTCGCATGGA GCTCAACCAA 
GCCAATGATA AAGAGAAGAG CCTTGCTGCT AAAGTGAAGA CTTTGGAGGC CCAAGTTGAT 
TTTGCTGACA GGCAACTGCG AGAGAAGAGA AGGATTGCAG ACGATGGTGA GGATTTGAGA 
CAAAAAGAAA ATCTGTACTT CAGAGTCCCA GAGAAGCAGC AAGACACCAG TGCAGACAGC 
CTGGAGTTTG AGCTAAACGA TTCTCTCAAC ACTACCTGTC GTTCTGCAGT ACCGGGTGAG 
TCCAGCACTC CACTGGTCCG TAGTTCTGAG CGTTTGGCTG CTAAACGCCG CGCATTGGGG 
GCTGAATCTC TCGAGACACT ATATTTCACA CCTATGGGTA AACAAGGCTA CAAACGCAAA 
GTGGATCACA ATGATGGTTT TGAACGCAAA CTGGAGTCCA GCATCACTTC TATTGGTGAT 
CTCGTGGTTG ATTCTGCAAA GAAATTGACT GCTTCAGCTC GCCGGCGACG CACTACACAA 
GTTATCAACA TCACCTTGGC TAAGAAGGCA CAAGGAAGCT CTGAAAGCGA GGAGTCGTTT 
TCTAGCCTGC ACTCAGCCCG TTCTCATCCA AATTTGGCAG TCCACCAATC ACGGCCGATC 
TCCATTGACT TCTCAGAGGA TGGTCCTGCC ACAACGTTAT CAAAAGCAGA CACACTTCAA 
AATCTGCCTG GATATCGCAG AAGTACAGTG CACAGCGTTG GCCCACCAAG AGCCACCAGT 
ACTTTTTGTA TTGGAGCAGA GAATGAACCG GAGCATGCTG CCGATGACTG GATGCGCATC 
GCTGAACTGC AGTCCCGAAA CAAAGCCTGT CTGCCTCATC TGAAGAGCAG CTATCCTCTG 
GAGTCCAGGC CCAGTCTAGG ACCATCATTC ACTGTAACGG ATGAGGACCT GCGCTTGGGC 
GACCCGAGTG AGACGATCCG GCGTGCGTCC ATGATGCCGG GTCAGATCCT GGAGTCGCTG 
AACTCTCGCC GTTTCTCCCT CGCTCCTGAA ACGAGTTCGA GCCAGGCTCC TGCACGCTCT 
CATCCCCAGC GAGCCACCAT GCTGCCTTGT CAGATCCAAT CCAGCACTGC TGCTCACCGG 
GCCTCACAGC TCACAAAAAC AAGCTCACGT TCACACACTT CAGAGAATAA ACGCAGCCCG 
CTGGCTCCCA AACGCCCGGC ATCACAACTG CAAGGACCAG ACACTCCTGA GGCAAAGAAG 
TTGGCAAGTT GTTTCCCACG GCCAATGACG CCCAAAGGCC GGTTTGGAAA CAGCCAAAAC 
AGACCTCCAG TCTCTCCGGC TGAGCGAAGA CAGTCTGTGA TGTTTACTGT GGTAAATACT 
CCAAAGAACA GTGCTCGTGG AGACAGCAGG CTTCAGCGCG GCCTCAACAA GCTGCGTAAC 
AGCACCCGCA AATCTCCTGC CGTGGCCAGT CGGGCTTTGC GCTCGGCCGT GAGCGGGAAA 
TCTCCCCTGG ACGTCACTCT GAGGAAATCT CCCCGAAACA AGTCCCCGAA AACATCCAAT 
GCCAAAAAGG AAACACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290241.1
CDS	65..7405
Translation	MKFNKNKAGPLLTWMNSVFPENQIWEFGHMRDGFRLLEICFKLKGREDVEAYKTLSLYNKLEIIFNDLHDDFHLTDRQRFLILGKISEGLDLELQLAKVALLLCYCSFKKTNPVALNSRTESDITFMFRFVRDDASGLCLDEGLDQFLDQDSVMNLANSSSGSSCSSPFYIDEESPMFGRFQRPPRVQFQELCTVASSSDSSPMQDLMSTPHFQLKKLRKELAHEGDVRDELEKELADHISIISEKEGLISQLQHRVERMLREQGELEKDHKAALLELQEKNESLLHRVHEVLKQCQDLKTDNSQKEKKIDELTEENGTLSAQLRNVFSQLARAEQEVAKLTVVHESAQTEWKNRKELLEKELNEAVTHKECLSEQVLILQGKISVLEDELKKAQSQERGEVLGPIMEWEKLKQELADLTLQLAQLQESISHLEKKKAEVEALLAEERCSFEKETARLQLVVFDLEKSINSIRLERETLQEALKTQKEMLSAQISALERDVSRLQQVEVQLTAEIKISADLRQQREELEGKVASLDRMVHSLHAEIQGLEVERASQQDAINSLISDLQIARATVQEYEKKLEEHQKVVAENNSLKNEACTMQQELNNHLKAIGDLQAEVNELRFEKTEEENKVSEALVKIESLQTEILHLCEKISLKDEEIRNLTKEYESVDNELKLVKEQNVEINAMIKSNRKEHEETVEKLQQELHCAASAASEKQEQMLVLSAEVTSLKEQICRYSENEAQKQQELSILEAQHNVLKENLTSLQNQLAEATTSASQKESEFILLQQELSHQETLREQTQELEKIKREELEGIVSELQAKIIEVSSIASEREACVSSLQYEMKDQLSKAKQCEADLRNEMKEKVGNLQELLDAANHGIAEKEHHLESLNQSLKQMELLYCQKEEEIIASQQAKQQLENNIAEQKQQLDEIQQNREILRNERDHLSTRVSSLQEEIHRSQQDFSDLQTDHNVLKEKLVNLQDQVEQAMITASQKESELLLLQQELSHQETLREKALELEAIKREEFEKKVKELQDRILEVSTLASQREAQVISLENEMRDQQLSANQSKDNLCREWQEKLGILKGELDVVSRDAADKDHQLESLDQKLKEMEMVVLQKEKDVMETHQAKEDLEKRIAELEECKQKLEIMRNERDHLSTEVASLKEEIHSYQDTQMQKQQTISVLEVENNALKENMAALEKQLAEEITTASQKNSELQNKLHQQESLHEKAQELEIGRHEEFERRVRELQAKVQEVSTLASEKEAVAYSLQEELTGHLKAKVSEDDLRRVLEEKVETLQREIETASCDATSKDGLLQTLDQKLRQMEMLCQQKEDAVFEIQNSKEDLQKEMNELVSKNQELEGCLQHLEMVKKEKDLLSNEVTSLKEQINDQSLRAKQSEADLCIVFEEKIETLQGQLESSSCDVSDKEKHLQTLHQKVSQMDLLCQQKENAVLEMQNAKEDLQKKIDELVSEKQQLEGCLQNLEMVKSERDLLSTEVTSLKDQLNDQDLKAKQSEDDLRKVLEEKIKNLQGQLETASRDVSEKDQLLQTLDQKVTQMDQLCKQKEKYALDLLQVKENLEKRIGEITVEKQQLEGCQQNLETVSKDKDNLLNELTFLKVEIQSYQEKEVQMKHELSVLENEHNILQENLDTLQKQVVELTASASQKESELQNEVCKQEKLQEKAQKLEKDAGDLQAKILEISTLASEREAQISSLKDEINSQHLKAKQSEDDLLRVFEEKIENLQGQLEIARLDVSDKDQLLQTLNQKLKQMEELCKQKETCVLEMHQTKEDLEKKTVELQADKSQQNQQNLEMLRQEKDHLKSLNQTIRRECEASRKIRAELELNVEEQTASILALKKASQQWEEQNQELLEQLKAKTEAVEHYKAQVEKAMNHYNGKKQLLLEAQELNKTLEQSLEVSKREAKALETELTLARMELNQANDKEKSLAAKVKTLEAQVDFADRQLREKRRIADDGEDLRQKENLYFRVPEKQQDTSADSLEFELNDSLNTTCRSAVPGESSTPLVRSSERLAAKRRALGAESLETLYFTPMGKQGYKRKVDHNDGFERKLESSITSIGDLVVDSAKKLTASARRRRTTQVINITLAKKAQGSSESEESFSSLHSARSHPNLAVHQSRPISIDFSEDGPATTLSKADTLQNLPGYRRSTVHSVGPPRATSTFCIGAENEPEHAADDWMRIAELQSRNKACLPHLKSSYPLESRPSLGPSFTVTDEDLRLGDPSETIRRASMMPGQILESLNSRRFSLAPETSSSQAPARSHPQRATMLPCQIQSSTAAHRASQLTKTSSRSHTSENKRSPLAPKRPASQLQGPDTPEAKKLASCFPRPMTPKGRFGNSQNRPPVSPAERRQSVMFTVVNTPKNSARGDSRLQRGLNKLRNSTRKSPAVASRALRSAVSGKSPLDVTLRKSPRNKSPKTSNAKKETQ

Target ORF information:

RefSeq Version	XM_009291966.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nuclear mitotic apparatus protein 1 (numa1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291966.2

ORF Insert Sequence:

ATGAAGTTTA ATAAGAACAA GGCGGGACCA CTTTTGACAT GGATGAACAG CGTGTTTCCA 
GAGAATCAAA TCTGGGAGTT TGGTCATATG AGAGACGGCT TTCGACTCTT GGAGATCTGC 
TTCAAACTAA AGGGGAGGGA GGACGTTGAA GCTTATAAGA CTTTATCCTT ATACAACAAA 
CTGGAAATCA TCTTTAATGA TTTACATGAT GACTTTCACC TGACTGATAG ACAACGTTTC 
CTCATTTTGG GTAAAATCAG TGAAGGACTT GACCTGGAGC TTCAGCTTGC CAAGGTGGCT 
CTTCTCCTTT GCTATTGCAG TTTTAAAAAG ACTAACCCTG TAGCCCTGAA TTCAAGAACA 
GAGTCGGACA TCACTTTCAT GTTTCGTTTT GTAAGAGATG ATGCCAGTGG TTTGTGTTTA 
GACGAAGGCC TCGATCAATT TCTAGACCAA GACTCTGTTA TGAATCTTGC AAACTCCAGT 
AGCGGGAGCA GCTGCAGTTC TCCATTCTAC ATCGATGAGG AGTCTCCGAT GTTTGGTCGT 
TTCCAGCGTC CTCCCAGAGT TCAGTTTCAA GAGCTTTGCA CAGTGGCTTC CAGCTCAGAC 
AGCTCTCCGA TGCAGGATTT GATGAGCACG CCACACTTCC AGTTGAAGAA ACTCCGCAAA 
GAACTGGCGC ATGAAGGAGA CGTGAGAGAT GAGCTGGAGA AAGAGCTGGC CGATCACATC 
AGCATCATCT CAGAGAAAGA GGGTCTGATC TCCCAGTTAC AGCACCGTGT GGAGCGGATG 
CTGCGGGAAC AAGGAGAGCT GGAGAAAGAC CATAAAGCTG CACTGCTGGA GCTCCAGGAG 
AAGAACGAGA GTCTTCTCCA CAGAGTTCAT GAGGTTTTAA AGCAGTGCCA AGATTTAAAA 
ACAGACAACT CTCAAAAAGA GAAAAAGATT GATGAGCTGA CAGAAGAGAA CGGGACTCTC 
AGTGCTCAGC TTCGTAATGT TTTCTCTCAG CTGGCGAGAG CAGAGCAGGA AGTTGCCAAA 
CTCACAGTGG TTCATGAATC TGCACAGACG GAGTGGAAAA ACAGGAAAGA GTTGCTGGAG 
AAGGAGCTGA ATGAGGCCGT CACACACAAG GAGTGTCTGA GTGAGCAGGT GCTGATACTG 
CAGGGGAAGA TTTCTGTTCT GGAGGATGAA CTGAAGAAAG CTCAGTCTCA GGAGAGAGGA 
GAGGTGCTGG GGCCCATCAT GGAGTGGGAA AAGCTCAAAC AAGAGTTGGC TGATCTCACT 
CTTCAACTCG CTCAGCTTCA GGAATCCATT TCCCACCTCG AGAAGAAAAA GGCAGAAGTT 
GAGGCCTTGT TGGCTGAGGA GAGATGCTCT TTTGAAAAAG AAACCGCAAG ACTCCAGTTG 
GTGGTTTTTG ATCTGGAGAA ATCCATCAAC AGCATCCGTC TGGAGAGAGA AACACTTCAG 
GAGGCCTTAA AGACGCAGAA AGAGATGCTC TCTGCGCAGA TATCAGCACT GGAGAGGGAC 
GTTTCCCGTC TGCAACAGGT TGAAGTGCAG CTGACAGCAG AGATCAAAAT ATCTGCAGAT 
CTCCGCCAAC AGAGAGAGGA GCTGGAAGGA AAGGTAGCAT CTTTAGATAG GATGGTTCAT 
TCTCTGCATG CTGAGATCCA GGGGCTGGAA GTGGAGAGAG CTTCTCAACA GGATGCAATT 
AATTCCCTCA TTAGCGATTT ACAAATCGCT AGGGCTACTG TTCAGGAATA TGAGAAGAAG 
CTGGAAGAGC ATCAAAAAGT GGTAGCAGAA AACAATTCCC TGAAAAATGA AGCATGCACA 
ATGCAGCAGG AGCTGAATAA TCATCTAAAG GCAATCGGAG ACCTTCAGGC GGAAGTAAAT 
GAACTCAGAT TTGAGAAAAC TGAGGAAGAG AACAAGGTTA GTGAGGCTTT GGTTAAAATC 
GAAAGCCTCC AGACTGAGAT TCTGCATCTG TGTGAAAAGA TTTCTCTTAA AGATGAAGAA 
ATCAGAAACC TGACGAAAGA GTATGAGTCT GTAGACAACG AGCTGAAGCT TGTGAAGGAG 
CAAAATGTTG AAATAAATGC AATGATCAAA TCTAATCGCA AGGAGCATGA GGAAACTGTT 
GAAAAACTTC AGCAGGAGCT TCATTGCGCT GCTTCTGCCG CATCAGAAAA ACAAGAGCAA 
ATGCTGGTTT TATCAGCAGA GGTAACATCT CTGAAAGAGC AAATCTGCAG ATATAGTGAA 
AATGAGGCAC AGAAACAACA GGAATTGTCT ATTTTAGAAG CACAACACAA TGTGCTGAAG 
GAAAACCTGA CTTCTCTACA GAACCAGTTG GCTGAGGCGA CAACTTCAGC TTCACAGAAG 
GAATCAGAGT TTATTTTGCT TCAACAGGAG CTTTCCCATC AAGAAACACT AAGAGAACAA 
ACTCAGGAGC TTGAGAAGAT TAAACGTGAG GAGCTTGAGG GGATCGTGAG TGAACTTCAG 
GCCAAAATTA TTGAGGTTTC CTCTATTGCC TCAGAAAGGG AAGCTTGTGT GAGCTCCCTT 
CAATATGAGA TGAAAGATCA GCTATCGAAA GCCAAACAGT GTGAAGCTGA TCTACGCAAT 
GAGATGAAGG AAAAGGTTGG AAATTTGCAG GAACTTCTAG ACGCTGCTAA CCATGGAATT 
GCTGAAAAAG AGCATCACTT AGAGTCGTTG AATCAAAGTC TGAAGCAGAT GGAGCTTCTG 
TACTGTCAAA AGGAGGAAGA AATCATTGCA AGTCAACAAG CAAAGCAGCA ACTGGAGAAT 
AATATTGCTG AGCAGAAACA ACAACTAGAT GAGATTCAGC AAAATCGAGA AATACTAAGG 
AATGAAAGAG ACCATCTGTC TACTCGAGTA TCATCTCTCC AAGAGGAAAT TCATCGTTCC 
CAACAGGATT TTTCTGATTT ACAGACTGAC CACAATGTTT TGAAGGAAAA ACTGGTTAAT 
CTTCAGGATC AAGTAGAGCA GGCGATGATT ACTGCTTCCC AGAAGGAATC TGAACTCCTC 
TTGCTTCAAC AGGAGCTTTC CCATCAAGAG ACCCTGAGAG AAAAAGCTTT GGAGCTTGAG 
GCCATCAAGC GAGAAGAGTT TGAGAAAAAA GTGAAGGAAC TGCAGGACAG AATTCTAGAG 
GTGTCAACTC TTGCCTCTCA AAGGGAAGCC CAGGTAATTT CTCTTGAAAA TGAGATGAGG 
GATCAACAGT TGAGTGCCAA CCAATCTAAA GATAATCTCT GCAGGGAGTG GCAGGAGAAG 
CTTGGCATCT TGAAAGGAGA ATTAGATGTA GTCAGTCGAG ACGCTGCAGA CAAAGACCAT 
CAACTGGAAT CTTTGGATCA AAAGCTTAAA GAGATGGAAA TGGTTGTCCT TCAAAAAGAG 
AAAGATGTAA TGGAGACACA TCAGGCAAAG GAGGACCTGG AGAAGAGAAT TGCTGAGCTT 
GAGGAGTGCA AGCAGAAATT AGAGATTATG AGGAATGAAA GAGATCATCT TTCAACTGAA 
GTAGCATCTC TAAAAGAGGA GATTCATAGC TACCAGGATA CTCAAATGCA GAAACAACAA 
ACAATTTCTG TTTTGGAAGT TGAAAACAAT GCTTTAAAGG AAAACATGGC TGCCCTTGAG 
AAACAATTAG CAGAGGAGAT AACTACAGCT TCACAGAAAA ATTCAGAGCT TCAAAACAAG 
CTTCATCAAC AAGAGAGCCT TCACGAAAAG GCTCAAGAGC TTGAGATTGG CAGGCATGAG 
GAATTTGAGA GAAGAGTGAG AGAACTTCAA GCTAAAGTCC AAGAGGTGTC CACTCTTGCT 
TCTGAGAAGG AGGCTGTTGC TTATTCTCTT CAGGAGGAAT TGACGGGTCA TTTGAAGGCT 
AAAGTGTCCG AAGATGATCT CCGTAGAGTC CTGGAGGAAA AAGTGGAAAC CTTACAGAGA 
GAAATAGAAA CTGCTAGTTG TGATGCCACA AGCAAAGACG GTCTTTTGCA GACTTTGGAT 
CAGAAGCTGA GGCAGATGGA GATGCTTTGC CAACAAAAGG AGGATGCAGT TTTTGAGATC 
CAGAACTCAA AAGAAGACTT GCAGAAAGAG ATGAATGAAC TTGTTTCTAA GAATCAAGAA 
CTGGAGGGCT GCCTGCAACA TTTGGAAATG GTAAAAAAGG AAAAAGACCT CCTGTCAAAT 
GAAGTAACTT CTCTGAAAGA GCAAATAAAT GATCAGAGTT TGAGGGCCAA ACAGTCTGAA 
GCTGATCTTT GCATAGTCTT TGAGGAAAAG ATTGAAACCC TTCAAGGACA ACTGGAATCT 
AGTAGTTGTG ACGTCTCGGA CAAAGAGAAA CATCTGCAAA CTTTGCATCA AAAGGTTTCA 
CAGATGGATC TGTTGTGCCA GCAAAAGGAG AATGCAGTTC TTGAGATGCA GAACGCAAAG 
GAAGACTTGC AGAAGAAGAT TGATGAGCTT GTTTCTGAGA AACAACAACT GGAGGGCTGC 
CTGCAAAATT TGGAAATGGT GAAAAGCGAA AGAGACCTCT TGTCAACTGA AGTAACTTCT 
CTAAAAGATC AACTAAATGA TCAGGATTTG AAAGCCAAAC AGTCCGAAGA TGATCTCCGC 
AAAGTGTTGG AAGAAAAGAT TAAAAACCTC CAGGGACAAC TTGAAACTGC TAGTCGTGAT 
GTCTCCGAAA AAGACCAGCT TCTGCAGACT TTGGATCAAA AGGTTACACA GATGGATCAG 
TTGTGTAAGC AAAAGGAGAA ATATGCTCTG GATTTGCTTC AGGTAAAGGA GAATTTGGAG 
AAGAGGATTG GTGAGATTAC TGTTGAGAAA CAGCAATTGG AAGGATGCCA GCAGAATTTG 
GAAACAGTGA GCAAGGACAA AGACAACCTT TTAAATGAAT TAACCTTTCT TAAAGTTGAA 
ATCCAAAGTT ACCAGGAAAA AGAAGTGCAG ATGAAACATG AGCTTTCTGT TTTAGAGAAT 
GAACACAACA TTTTACAGGA GAACCTGGAT ACTCTTCAGA AGCAAGTGGT GGAGTTGACC 
GCTTCAGCTT CTCAGAAAGA GTCTGAGCTT CAAAATGAGG TTTGCAAACA AGAGAAACTT 
CAAGAAAAGG CTCAGAAACT TGAGAAGGAT GCAGGTGATC TTCAGGCTAA AATTCTAGAG 
ATTTCCACTC TTGCTTCTGA GAGAGAGGCA CAAATAAGTT CTCTTAAAGA TGAAATAAAC 
AGTCAGCATT TGAAAGCTAA ACAGTCTGAA GATGATCTCC TCAGAGTGTT TGAGGAGAAA 
ATTGAAAACC TTCAAGGACA GCTTGAAATT GCTCGTCTTG ATGTCTCGGA CAAAGACCAG 
CTTCTGCAAA CTTTAAATCA GAAACTGAAA CAAATGGAAG AACTATGCAA GCAAAAGGAG 
ACATGTGTCC TTGAGATGCA TCAGACAAAG GAGGATCTGG AGAAGAAAAC TGTTGAGCTT 
CAAGCTGACA AAAGTCAACA GAATCAGCAG AATTTGGAGA TGTTGAGGCA AGAAAAGGAC 
CATCTGAAAT CTCTAAACCA GACTATCCGA AGAGAGTGTG AAGCGTCCCG GAAGATTCGA 
GCTGAGCTGG AGTTGAACGT TGAGGAACAA ACTGCTTCCA TCCTTGCACT TAAAAAGGCT 
TCCCAACAGT GGGAGGAGCA GAATCAGGAG CTCCTGGAGC AGCTGAAGGC AAAGACAGAA 
GCTGTAGAGC ACTACAAAGC ACAGGTGGAG AAGGCCATGA ATCACTACAA TGGTAAAAAG 
CAGCTGCTGC TGGAGGCTCA AGAGCTGAAC AAAACTCTTG AGCAGTCATT GGAGGTCAGC 
AAGAGAGAAG CTAAAGCTCT GGAAACTGAA CTAACCCTGG CTCGCATGGA GCTCAACCAA 
GCCAATGATA AAGAGAAGAG CCTTGCTGCT AAAGTGAAGA CTTTGGAGGC CCAAGTTGAT 
TTTGCTGACA GGCAACTGCG AGAGAAGAGA AGGATTGCAG ACGATGGTGA GGATTTGAGA 
CAAAAAGAAA ATCTGTACTT CAGAGTCCCA GAGAAGCAGC AAGACACCAG TGCAGACAGC 
CTGGAGTTTG AGCTAAACGA TTCTCTCAAC ACTACCTGTC GTTCTGCAGT ACCGGGTGAG 
TCCAGCACTC CACTGGTCCG TAGTTCTGAG CGTTTGGCTG CTAAACGCCG CGCATTGGGG 
GCTGAATCTC TCGAGACACT ATATTTCACA CCTATGGGTA AACAAGGCTA CAAACGCAAA 
GTGGATCACA ATGATGGTTT TGAACGCAAA CTGGAGTCCA GCATCACTTC TATTGGTGAT 
CTCGTGGTTG ATTCTGCAAA GAAATTGACT GCTTCAGCTC GCCGGCGACG CACTACACAA 
GTTATCAACA TCACCTTGGC TAAGAAGGCA CAAGGAAGCT CTGAAAGCGA GGAGTCGTTT 
TCTAGCCTGC ACTCAGCCCG TTCTCATCCA AATTTGGCAG TCCACCAATC ACGGCCGATC 
TCCATTGACT TCTCAGAGGA TGGTCCTGCC ACAACGTTAT CAAAAGCAGA CACACTTCAA 
AATCTGCCTG GATATCGCAG AAGTACAGTG CACAGCGTTG GCCCACCAAG AGCCACCAGT 
ACTTTTTGTA TTGGAGCAGA GAATGAACCG GAGCATGCTG CCGATGACTG GATGCGCATC 
GCTGAACTGC AGTCCCGAAA CAAAGCCTGT CTGCCTCATC TGAAGAGCAG CTATCCTCTG 
GAGTCCAGGC CCAGTCTAGG ACCATCATTC ACTGTAACGG ATGAGGACCT GCGCTTGGGC 
GACCCGAGTG AGACGATCCG GCGTGCGTCC ATGATGCCGG GTCAGATCCT GGAGTCGCTG 
AACTCTCGCC GTTTCTCCCT CGCTCCTGAA ACGAGTTCGA GCCAGGCTCC TGCACGCTCT 
CATCCCCAGC GAGCCACCAT GCTGCCTTGT CAGATCCAAT CCAGCACTGC TGCTCACCGG 
GCCTCACAGC TCACAAAAAC AAGCTCACGT TCACACACTT CAGAGAATAA ACGCAGCCCG 
CTGGCTCCCA AACGCCCGGC ATCACAACTG CAAGGACCAG ACACTCCTGA GGCAAAGAAG 
TTGGCAAGTT GTTTCCCACG GCCAATGACG CCCAAAGGCC GGTTTGGAAA CAGCCAAAAC 
AGACCTCCAG TCTCTCCGGC TGAGCGAAGA CAGTCTGTGA TGTTTACTGT GGTAAATACT 
CCAAAGAACA GTGCTCGTGG AGACAGCAGG CTTCAGCGCG GCCTCAACAA GCTGCGTAAC 
AGCACCCGCA AATCTCCTGC CGTGGCCAGT CGGGCTTTGC GCTCGGCCGT GAGCGGGAAA 
TCTCCCCTGG ACGTCACTCT GAGGAAATCT CCCCGAAACA AGTCCCCGAA AACATCCAAT 
GCCAAAAAGG AAACACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

The Fz-Dsh planar cell polarity pathway induces oriented cell division via Mud/NuMA in Drosophila and zebrafish.
Developmental cell19(5)740-52(2010 Nov)
Ségalen M,Johnston CA,Martin CA,Dumortier JG,Prehoda KE,David NB,Doe CQ,Bellaïche Y

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NUMA1 cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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