TPRA cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	TPRA
Entrez Gene ID	571089
Full Name	translocated promoter region a, nuclear basket protein
Synonyms	si:ch1073-412d11.1
General protein information	Preferred Names translocated promoter region a, nuclear basket protein Names nucleoprotein TPR translocated promoter region a, nuclear basket protein translocated promoter region a (to activated MET oncogene)
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009298497.3	XP_009296772.1	nucleoprotein TPR isoform X1
XM_009298498.3	XP_009296773.1	nucleoprotein TPR isoform X2
XM_009298497.2	XP_009296772.1	nucleoprotein TPR isoform X1
XM_009298498.2	XP_009296773.1	nucleoprotein TPR isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC101882241	XP_005163419.1
Danio rerio (zebrafish)	tpra	XP_003197898.2

Gene Ontology

The following TPRA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TPRA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa46084	XM_009298497.3 Latest version!	Danio rerio translocated promoter region a, nuclear basket protein (tpra), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa46085	XM_009298498.3 Latest version!	Danio rerio translocated promoter region a, nuclear basket protein (tpra), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
ODa46084	XM_009298497.2	Danio rerio translocated promoter region a, nuclear basket protein (tpra), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa46085	XM_009298498.2	Danio rerio translocated promoter region a, nuclear basket protein (tpra), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TPRA ( XM_009298497.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296772.1 Danio rerio translocated promoter region a, nuclear basket protein (tpra), transcript variant X1, mRNA.

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CloneID	ODa46084
Clone ID Related Accession (Same CDS sequence)	XM_009298497.3 , XM_009298497.2
Accession Version	XM_009298497.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	nucleoprotein TPR isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298497.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCAC TTCTTCAGCA AGCGCTGGAA AGAACCGAGG TCAACAAACT GCCGAAAGCC 
ATTCAGAACA AGCTCGAGAA GGTTCTGGCA GAGCAGCAGA CGGAAATCGA CTCGTTGATA 
TCACAGCATG AGCGGTATAA AGCAGACTGT GAGCAACAGT ACTTTAATCT CGAGAAGAAG 
CTTGCTGAGA GTCAGGAGCA GTTTCTATCC CAAAGTAAGG ACTACCACAA TATTAAGGAG 
GAAAACAACC GTCTTGTGGA AGAGCTTAAG AAGTTAAAAG AGATAGAAGA AGTGCAGGGA 
ACAACGCAAG AGAGTAAGCC TCCTAGAGCC AAATATGAAA TCGAGGCAGA AAACCGAGAG 
CTGTCACGAT TGCTTGAGAA GAGATCTCAA GAAGTGGAAA ACCTCAGTGA GGATCTCAAA 
CGTCTCAATG ACAAACTTGT GGAGACCAAC ACAATCAAGA TGGAGCTCCA GCTTAAACTA 
GATGAACTTC AGTCTTCTGA GGTTTCCATT CAGTATCGTG AGAAGCGGAT GGAACAAGAA 
AAAGAGCTTC TCCAGAACCA GAACACATGG CTGAACACAG AGCTGAAGAG TAAGACGGAT 
GAGCTGTACA CCGTATCCAG AGACAAAGGC AAAGAGAACC TGGAGCTTAG GTGTTCTTTA 
GAGAGCAAGA AGGAAGAGGT GACCAGACTT CAGGATCAAG TAAACACACT CAAGAAAGCA 
AATGACAGCA TGCAGAAGAG TACAGAGGAT CTGATGAGGA AGCTAAAAGA GGCTAAAGAA 
CAACGGGCCT CAATGGAAGA GAAGTACCGC AATGAGCTGA ATGCAAACCT CAAACTATGT 
AATTTGTACA AGGGTGCAGC ATCAGATTCT GAAGCCAAGA ACACTGAACT GAACAGAGCT 
GTGGAAGAGC TCAATAAACT GCTGAAGGAA GCAATGGAAG CCAATAAAGG AACCGAGAAG 
AAGCTGTCAG AGTTGCAGAG CGTAAGGGAG AAAGCAGAGT CAGATTTACG TGAGAAGGTT 
AGGCATCTGG AGAAGGAGCT GGAGAACTCC AACACAAGGC TCGCAGACTT TAAGAGGAGA 
GGTGTTCCAG CTCTCACAGA AGAGGAGCTG ACAAACCTTT CTCCAACTGC AGCGGCAGTG 
GCCAAGATCG TGAAGCCGGG CATGAAGCTA ATGGAGTTGT ACAATGCCTT TGTGGAGGCA 
CAAGACCAGC TGCACCTTGA GAAGTTGGAG AACAAGCGTG TGAACAAGGT CCTTGATGAG 
ATCGTGCTGG AGGTGGAAAC CAAAGCTCCC ATCCTGAAAC GCCAGAGAGA GGAATATGAG 
AGCATGCAGA AGTCTATGAC CAGTCTCTGT TATAAACTAG AGCAAGCTAT GAAGGAGGTC 
CATCGGCTAC AAAAAGAAAC GGATGAAGCC AATAAGAGAG CTTTAGTGCT TGAAAGAGAT 
AATCAGAAAT CAGAGAGACA GCTAGCCGAC ATGTCTGAGC AGGTGCGTGT GCTTCTGGTG 
GAGGTGGAGG AGGCACGTGG TAATCAGGTA GTGCGTGAGG ATGTGAGTTC TGCTGTGAGC 
AGTAGCTCTG AGGTCCAGGG TTCACGACAG GTGGCATTCC GCAGCATCCA AGAGCTTCAG 
CAGCAGAACC AGAACTTACT GGTCAAGATC AGAGACCTGG AAGAGCAGAG AGAAAAAGAC 
CAGAACCAGG CTAAGAGTGC AAAGCAAACA GAATTGGAAC AAAGTCTGGA GAAGGTTCAG 
AAGGAGCTGG AGCAGATCAA GGAACAGCAA AACCATCAGA AGCTGCTGGC TGAATCTGCC 
ATCCGCCAGA GAGACATGTA CCGTATCCTT TTGCAGACAG CCGGAGTCGA GCTTCCACCA 
CAGGGTTCAG ATGGTGGATC TCAATCCACT ACTCCCAGCA GGCCAGGACC CATGGCCACT 
AGATCCACAC CACTAAGGGC TGCTGCTGCA GAATCTTTCC AGGCAACCCA GTCTAAAGCA 
GCACTTAAAC AGATAAATGA TGCCTTTACT ACATATAAAA AGGAGAAGGC CGAAAATGAC 
AAGCTTCTAA ATGAGCAGAT TGAGAGGCTT CGTGGTCAAG TGTCTGACCT GCTCTCACAA 
AAGGCCAAAC TGTCCTCTCA GCTGGAGTTT GCCTCTAAAC GGTATGATAT GCTTCAGGAG 
AATGTGAATG GCTTTCGAAG AGAGACTGAA GCCCTGCGGG AGAGGAATCA AAAGATGTCT 
GCCACCCATC AGAGACATGA GCAAATCATT CACACAATGA CTCAAGACCT GAGGGAGGCC 
AATGAGAAAC TGACCATGTC CGAGTCGCGG GCTGAAAACA TCCGTAAGGA ACGAGACATT 
CTGAAACAGG TGGAAAACAG ACTCAACCAG GAGAAAGAGT CTATCCTGAC TGAACAGCGG 
AGCCAGAACC TTCTGCTCAC CAACCTCAAA TCCATTCAGC TCACCATGGA GCGCTCTGAA 
ACTGAGACCA AACAGCGCTA CAATAATCAA ATCCAGCATC TGGAAAAGGA GATTATCCAG 
TTAAAGAAGA GGCTGGAGCA AGAAGTGGAA CAGAGACACG CACTGGAACG CAACCAAGAT 
ACTCAGCTGT TGGAGGCTAA GAAGAAGCTG GAGTCACAGA CCGCACTACA CCAGAAGACT 
AAAGAGCTGC TGAGATCTGC TGAACAGCAG GTGACCTCTT TGAGACAGCA ACTGAACAAC 
AATGAGAATC AGAGTGCAGC ACCTAAGCAG CCTGTCAGGA CATTACCCAG AGTTTCAGCT 
CCCAGCGTTC CTCAACAGGA GGCAGATGAT CTTCGTGCAC GTCTACAGCA GGTCGAGGAA 
CAGAACTCTG ACCTGAAAGA ACGCTTGAAG AACACCACCA CCAATGTAGA GCAGTATAGA 
TCTATGGTGC TCAGCTTGGA GGAGTCTGTG AACAAGGAGA AACAGTTGGC TGAGCAAGCA 
CGGACCTCCA CTGACAACCA ACTGAAGATG GCTCGGGAGT TGAATCAGCA GCTGGAAGCC 
CGTTTGGTGG AAGCGGAGAA GGAGAAGTTG GAGCTCCAGC AGGAGAAGAT GAAAGCTGTG 
GATAGTGTTG AAGAACGGGT GAAGGAGCTG AAAAGGAGCA TGACAAATAT GCAGACTGAG 
CTTCAGGAAG CTCTGCAGAG ATCTACAGCA GCAGTCACTC AGGAGCAGAA AGCCACACAG 
GACAGCAAAG TACAGGCTAA GCTGGCTGCA GAGGCTCAGA ATAAATACGA GCGAGAGCTC 
ATGCTGCATG CTGCAGATGT GGAAGCCCTG CAGGCCGCCA AGAAACAGGG ACAGCTTTTA 
ACACAAAACA TGAAGCAGCT CGAGGAGAAA GCTCAGAAAG CTGCATCTGA ACTGCATCAG 
TACCGCACAG ACTGGGTAAT GCAGGAGAAG AGACTAAAGG AAACGCTTTC TAAAGAGCAG 
AAGAGAGCCG AGGAGCTGCA GAAGCAGAAC ACTTTGCTCC ATGAGCAGAT GGAGAACTTG 
AGCAGCAAGA TCACTGCCTC AACCCAGCAG GAGACTGGAG AGAGCAGTCT GAACATCTCT 
CTGAACGAGG AGGGCAAGTC TCATGAGCAG ATACTGGACA TCCTCAGGTT TGTCCGGCGG 
GAGAAGGAGA TTGCAGAGAC CCGACTGGAA GTGGCCGAGG TGGAGACCCT TCGCTACAAA 
CAGCGCATGG AGTATCTGGA GTGCGAGCTG AAGGAACTAC AGGACAGCCT GAATGTAGAG 
AGAGAGAAGT TGCAGGTGAC AACAAAGACC TTGGTACAGC AGGAAGAGAC GATGAAGAGG 
ATGGAGAGCA TGAGTGCTCT TACAGATACC AACAAAATGC TGAAAAATGA GAAAAATCGA 
CTGGAGCATG AGCTGCAGCA GACTCAGGCC AAGATGCGTA AGCTCGAAGC AGACATCAAG 
CCTCTGCAGG AGGCCAATGG TGAGCTGAGT GAGAAGAGTG GCATGTTACA GGCTGAGAAG 
AGGCTTCTGG AGGAGGATGT CAAGCGCTTG AAGTCTCGCA CACAGCAACT CTTGAATCAG 
CAGAAGGACA GCGATCAGGA GGAAAGCAAG AAGCTTCATT CTGAGAGAGA GACTCATCTT 
AAACGCATCC AGCAGCTCAC AGAGGAGACT GGCAAACTGA AGAATGAGGT GGCCCGGAGC 
CAAGCTTCAA TCACCACGGC TCAGAGCCAA GTGCAGAACC TGCAAGACAG TCTGGGGAAG 
GTCACCACTG AACGAGACAG TCTGAAGAAG GAGCTGGAGG CAAAAACACT GGACATCCAG 
GAGAAAGTCA AAACCATCAC GCAGGTCAAG AAAATCGGCC GTCGCTACAA AACCCAGTAT 
GAAGAGCTCA AAGAACAGCA TGATAAGATG GTGGCAGAGG CTGCTTCTAA ACCAGCCCAG 
GAGCAGGAAG ACCGTCAGGC CTCAGTGCAG GAGATTCAGA ACCTGAAGAG CTCATTGAGT 
CAGACACAGA ATCGAACCTC AGAGCTGGAA ACTCAGCTGG ACTGCATACA GAAGACGTTG 
CAGGAGCGTG AAGCAGAGGT GAAGAGCCTA CAGGAACAGC TGACACAAGT GCAGCCTGAG 
CTCAGCCGTC TAAAAGCAGA ACTGCAGGAG AAGGGCAAAC TGGAGGAGCA GCTCCGACAG 
CAGATCGCTG ATAAAGAGGA GAAGACCAAG AAAGCCTTCT TGGGGGCCAA ACAGAAGCTC 
AACCAGCTCA ACAGTGCAAA GGAGCAGCTG GCGAAAGAAA ACGAGGAGCT GAAGCTGCAG 
CGAGAGGAGC TGGAGGTGAG GATGAGTGCA CTGAAGTCTC AATATGAAGG TCGACTTAGC 
CGACAAGAAC GAGAGCTGAG GGAACTTCGT GAACAGCAGG AGAGACATGG AGAACAGAGA 
GATGAAGCTC AGGAGCCTGG CCAGAGCAAG GTTCAAGAGG CACCGAGGTC ATCAGATGCT 
CGTCAGATCA CACTCAAGCC TACGCCTGCT GCTGACAGAG GAAGTGCAAG TCAGTCTGAA 
CCACCCACTG CCAACATCAA GCCCACGTTA GTTGCCGCCA CCCCTAGCAA GCCTTCACCA 
ATCGCAGGCA GCAAGTCCAC TCCTAAAGCA AGCATCCGTC CAATGATAAC GCCTGCTCCT 
GTTACCACAC CCACAGCCAC AGTCATGCCC ACCACACAGG TTGAAACGCA GGAAGCTCTA 
CAATCCACAG AGGGTCCGCT GGAGCATGTG ACTGTGTTTG GCAGCACCAG TGGCTCAGTG 
CGCTCCACCA GCCCAAACAT ACAGACCACA CAGCCAATCC TCAGCCTGCA GCAGAGCCAA 
GCCACTGCCT TCGTCCAGCC CACTCAGCAG CAGGCCAGCC AGGAACCAGC AACCAGCATT 
ATGGAAGCGG TGCATAGCTC TCAGATGGAG AGGCCCTCCA CCTCCACCGC TGTCTTTGGT 
ACAGTTTCTG CAACAACGGG TTCTTCGGTG GCCAAGCGAG TGCGAGAGGA GCATGAGGAG 
AGCTCCACTG AGGTGACGGA CACCACACAG GATGACTCTA CACATCCACC CATCATGAAG 
AAGCTCCGCA TTGTAAGAAG AGTGGGTCTG GAGGATGCTT CAGGTACTGA GGAGAGTAAT 
GATGCTATGG GTGAATCTTC AGGAGACGTT CAGCAGCCTC CTGATGCCAG TGAGGAAGCC 
TATCCCGTAT TAGGAGAGGG GGATGAAGAA TCATTGTCTG CCCCCATGGA TCAGGTGAGT 
GAGTCTCAAC CCTCTGAGTC TCAGATCAGT GGACAGGACT CATCAGAAGA GTACAAGCAT 
GATGTAATCG TCATCGAAAC AGACAGCGCC ACTGAGGAGG AGGAGGAAGA GGAAGAGGAG 
CAGGAAGAAC CTGGACAGTA TGAAGATGAT GATGAGGATG AGGACGATGA CGCAGAAGAT 
GAGGATGATG AAGATGGGGA TGGAGGAATG GCTGAAGCAG CAGAGGAGAG TAATGAAGGC 
AGTGGGAGTG CAGATGCCAA TGAGGCTTAT GAGGGGGATG AGGCAGAGCA GGGTGCCGGG 
GAAGCTGATC CAAGTGTTCA CAATGAAGAG AGTCAAGAGG CATCTGATTC AACCCAGAAG 
CAACCAGACT CTCAGAGCAG TGAAGCTAGT GTCAGCACCC TGGAGGCCTT CCCTCTTGAG 
CCTGTAAGAG AGCACCCGCC GCTGCCCAGC ACTACCACCA CCTCCCTCGC CCCGCGTCTG 
CCCCAATCCC CCCGTAGACC ACATCACACC CCTCCACCTC GTCTTAATAT CCACCCTGTC 
CCTGAACTTG GACCTCCTAT CATGCAGAGG CAGTCTGGAC AGAGCAGACG ACCATCAATG 
AGTCGCGCAC CGCAGCTTAC GCCCGGTATT GGAAGCATGC AACACTTCTT TGATGACGAT 
GACAGAATGG TTCCCAGCAC TCCCACTCTT GTGGTGCCAC ACCGCACAGA TGGCTTTGCG 
GAGGCCATTC ATTCCCCTCA GGTGGCTGGA GTTCCTCGCT TCCGTTTCGG CCCCCCAGAG 
GACATGATGC CTCAGGCCAG CTCTTCTCAT TCTGACCTGA GCCATCTGGC CACACATGGA 
GGACTGGGCA TGTATGAAAG TCCACTGTTC CTGCCCACTC ATGATGAGGA GTCTGGAGGC 
AGGAGTGTGC CCACCACCCC TCTACAAGTG GCCGCTCCAG TGACCGTGTT CACCGAAAGT 
CACCCGTCGG ATGTTTCGGA GACGGCCTCT CAGTCAGTGC CCATGGTAAG CACATCCACC 
GCCAGCCTGA GTGCACCAGG AGAGGCTGTG CCAGGAGATG ATGGAGATGA CGTGTTCGTG 
GCTGAGGCTG AGAGCGAAGG GGTGAGTAGT GAAGCGTGTC TAGAAAGCCA GTTGGAGCTA 
GATTCTGCAC AACCGGCTGA TGATGCCAGT CTTCCCTCCA CCAGTCAAGA GCCTACGTCT 
AGTTCTGCAG ACACTAGCAC CAGTAGCGGT CAGGCTAAAC CCGCAGGCCG TCTGCTTCCT 
CAGCACCATC TGCCCCGGAG AAACCAGCTG ATCCGGAGAG GTTTTCCTCG TGGAGGTCGG 
GGTCGAGGGC TCAGCAGAGG CTCCCCTTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296772.1
CDS	56..7108
Translation	MAALLQQALERTEVNKLPKAIQNKLEKVLAEQQTEIDSLISQHERYKADCEQQYFNLEKKLAESQEQFLSQSKDYHNIKEENNRLVEELKKLKEIEEVQGTTQESKPPRAKYEIEAENRELSRLLEKRSQEVENLSEDLKRLNDKLVETNTIKMELQLKLDELQSSEVSIQYREKRMEQEKELLQNQNTWLNTELKSKTDELYTVSRDKGKENLELRCSLESKKEEVTRLQDQVNTLKKANDSMQKSTEDLMRKLKEAKEQRASMEEKYRNELNANLKLCNLYKGAASDSEAKNTELNRAVEELNKLLKEAMEANKGTEKKLSELQSVREKAESDLREKVRHLEKELENSNTRLADFKRRGVPALTEEELTNLSPTAAAVAKIVKPGMKLMELYNAFVEAQDQLHLEKLENKRVNKVLDEIVLEVETKAPILKRQREEYESMQKSMTSLCYKLEQAMKEVHRLQKETDEANKRALVLERDNQKSERQLADMSEQVRVLLVEVEEARGNQVVREDVSSAVSSSSEVQGSRQVAFRSIQELQQQNQNLLVKIRDLEEQREKDQNQAKSAKQTELEQSLEKVQKELEQIKEQQNHQKLLAESAIRQRDMYRILLQTAGVELPPQGSDGGSQSTTPSRPGPMATRSTPLRAAAAESFQATQSKAALKQINDAFTTYKKEKAENDKLLNEQIERLRGQVSDLLSQKAKLSSQLEFASKRYDMLQENVNGFRRETEALRERNQKMSATHQRHEQIIHTMTQDLREANEKLTMSESRAENIRKERDILKQVENRLNQEKESILTEQRSQNLLLTNLKSIQLTMERSETETKQRYNNQIQHLEKEIIQLKKRLEQEVEQRHALERNQDTQLLEAKKKLESQTALHQKTKELLRSAEQQVTSLRQQLNNNENQSAAPKQPVRTLPRVSAPSVPQQEADDLRARLQQVEEQNSDLKERLKNTTTNVEQYRSMVLSLEESVNKEKQLAEQARTSTDNQLKMARELNQQLEARLVEAEKEKLELQQEKMKAVDSVEERVKELKRSMTNMQTELQEALQRSTAAVTQEQKATQDSKVQAKLAAEAQNKYERELMLHAADVEALQAAKKQGQLLTQNMKQLEEKAQKAASELHQYRTDWVMQEKRLKETLSKEQKRAEELQKQNTLLHEQMENLSSKITASTQQETGESSLNISLNEEGKSHEQILDILRFVRREKEIAETRLEVAEVETLRYKQRMEYLECELKELQDSLNVEREKLQVTTKTLVQQEETMKRMESMSALTDTNKMLKNEKNRLEHELQQTQAKMRKLEADIKPLQEANGELSEKSGMLQAEKRLLEEDVKRLKSRTQQLLNQQKDSDQEESKKLHSERETHLKRIQQLTEETGKLKNEVARSQASITTAQSQVQNLQDSLGKVTTERDSLKKELEAKTLDIQEKVKTITQVKKIGRRYKTQYEELKEQHDKMVAEAASKPAQEQEDRQASVQEIQNLKSSLSQTQNRTSELETQLDCIQKTLQEREAEVKSLQEQLTQVQPELSRLKAELQEKGKLEEQLRQQIADKEEKTKKAFLGAKQKLNQLNSAKEQLAKENEELKLQREELEVRMSALKSQYEGRLSRQERELRELREQQERHGEQRDEAQEPGQSKVQEAPRSSDARQITLKPTPAADRGSASQSEPPTANIKPTLVAATPSKPSPIAGSKSTPKASIRPMITPAPVTTPTATVMPTTQVETQEALQSTEGPLEHVTVFGSTSGSVRSTSPNIQTTQPILSLQQSQATAFVQPTQQQASQEPATSIMEAVHSSQMERPSTSTAVFGTVSATTGSSVAKRVREEHEESSTEVTDTTQDDSTHPPIMKKLRIVRRVGLEDASGTEESNDAMGESSGDVQQPPDASEEAYPVLGEGDEESLSAPMDQVSESQPSESQISGQDSSEEYKHDVIVIETDSATEEEEEEEEEQEEPGQYEDDDEDEDDDAEDEDDEDGDGGMAEAAEESNEGSGSADANEAYEGDEAEQGAGEADPSVHNEESQEASDSTQKQPDSQSSEASVSTLEAFPLEPVREHPPLPSTTTTSLAPRLPQSPRRPHHTPPPRLNIHPVPELGPPIMQRQSGQSRRPSMSRAPQLTPGIGSMQHFFDDDDRMVPSTPTLVVPHRTDGFAEAIHSPQVAGVPRFRFGPPEDMMPQASSSHSDLSHLATHGGLGMYESPLFLPTHDEESGGRSVPTTPLQVAAPVTVFTESHPSDVSETASQSVPMVSTSTASLSAPGEAVPGDDGDDVFVAEAESEGVSSEACLESQLELDSAQPADDASLPSTSQEPTSSSADTSTSSGQAKPAGRLLPQHHLPRRNQLIRRGFPRGGRGRGLSRGSPF

Target ORF information:

RefSeq Version	XM_009298497.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio translocated promoter region a, nuclear basket protein (tpra), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298497.3

ORF Insert Sequence:

ATGGCGGCAC TTCTTCAGCA AGCGCTGGAA AGAACCGAGG TCAACAAACT GCCGAAAGCC 
ATTCAGAACA AGCTCGAGAA GGTTCTGGCA GAGCAGCAGA CGGAAATCGA CTCGTTGATA 
TCACAGCATG AGCGGTATAA AGCAGACTGT GAGCAACAGT ACTTTAATCT CGAGAAGAAG 
CTTGCTGAGA GTCAGGAGCA GTTTCTATCC CAAAGTAAGG ACTACCACAA TATTAAGGAG 
GAAAACAACC GTCTTGTGGA AGAGCTTAAG AAGTTAAAAG AGATAGAAGA AGTGCAGGGA 
ACAACGCAAG AGAGTAAGCC TCCTAGAGCC AAATATGAAA TCGAGGCAGA AAACCGAGAG 
CTGTCACGAT TGCTTGAGAA GAGATCTCAA GAAGTGGAAA ACCTCAGTGA GGATCTCAAA 
CGTCTCAATG ACAAACTTGT GGAGACCAAC ACAATCAAGA TGGAGCTCCA GCTTAAACTA 
GATGAACTTC AGTCTTCTGA GGTTTCCATT CAGTATCGTG AGAAGCGGAT GGAACAAGAA 
AAAGAGCTTC TCCAGAACCA GAACACATGG CTGAACACAG AGCTGAAGAG TAAGACGGAT 
GAGCTGTACA CCGTATCCAG AGACAAAGGC AAAGAGAACC TGGAGCTTAG GTGTTCTTTA 
GAGAGCAAGA AGGAAGAGGT GACCAGACTT CAGGATCAAG TAAACACACT CAAGAAAGCA 
AATGACAGCA TGCAGAAGAG TACAGAGGAT CTGATGAGGA AGCTAAAAGA GGCTAAAGAA 
CAACGGGCCT CAATGGAAGA GAAGTACCGC AATGAGCTGA ATGCAAACCT CAAACTATGT 
AATTTGTACA AGGGTGCAGC ATCAGATTCT GAAGCCAAGA ACACTGAACT GAACAGAGCT 
GTGGAAGAGC TCAATAAACT GCTGAAGGAA GCAATGGAAG CCAATAAAGG AACCGAGAAG 
AAGCTGTCAG AGTTGCAGAG CGTAAGGGAG AAAGCAGAGT CAGATTTACG TGAGAAGGTT 
AGGCATCTGG AGAAGGAGCT GGAGAACTCC AACACAAGGC TCGCAGACTT TAAGAGGAGA 
GGTGTTCCAG CTCTCACAGA AGAGGAGCTG ACAAACCTTT CTCCAACTGC AGCGGCAGTG 
GCCAAGATCG TGAAGCCGGG CATGAAGCTA ATGGAGTTGT ACAATGCCTT TGTGGAGGCA 
CAAGACCAGC TGCACCTTGA GAAGTTGGAG AACAAGCGTG TGAACAAGGT CCTTGATGAG 
ATCGTGCTGG AGGTGGAAAC CAAAGCTCCC ATCCTGAAAC GCCAGAGAGA GGAATATGAG 
AGCATGCAGA AGTCTATGAC CAGTCTCTGT TATAAACTAG AGCAAGCTAT GAAGGAGGTC 
CATCGGCTAC AAAAAGAAAC GGATGAAGCC AATAAGAGAG CTTTAGTGCT TGAAAGAGAT 
AATCAGAAAT CAGAGAGACA GCTAGCCGAC ATGTCTGAGC AGGTGCGTGT GCTTCTGGTG 
GAGGTGGAGG AGGCACGTGG TAATCAGGTA GTGCGTGAGG ATGTGAGTTC TGCTGTGAGC 
AGTAGCTCTG AGGTCCAGGG TTCACGACAG GTGGCATTCC GCAGCATCCA AGAGCTTCAG 
CAGCAGAACC AGAACTTACT GGTCAAGATC AGAGACCTGG AAGAGCAGAG AGAAAAAGAC 
CAGAACCAGG CTAAGAGTGC AAAGCAAACA GAATTGGAAC AAAGTCTGGA GAAGGTTCAG 
AAGGAGCTGG AGCAGATCAA GGAACAGCAA AACCATCAGA AGCTGCTGGC TGAATCTGCC 
ATCCGCCAGA GAGACATGTA CCGTATCCTT TTGCAGACAG CCGGAGTCGA GCTTCCACCA 
CAGGGTTCAG ATGGTGGATC TCAATCCACT ACTCCCAGCA GGCCAGGACC CATGGCCACT 
AGATCCACAC CACTAAGGGC TGCTGCTGCA GAATCTTTCC AGGCAACCCA GTCTAAAGCA 
GCACTTAAAC AGATAAATGA TGCCTTTACT ACATATAAAA AGGAGAAGGC CGAAAATGAC 
AAGCTTCTAA ATGAGCAGAT TGAGAGGCTT CGTGGTCAAG TGTCTGACCT GCTCTCACAA 
AAGGCCAAAC TGTCCTCTCA GCTGGAGTTT GCCTCTAAAC GGTATGATAT GCTTCAGGAG 
AATGTGAATG GCTTTCGAAG AGAGACTGAA GCCCTGCGGG AGAGGAATCA AAAGATGTCT 
GCCACCCATC AGAGACATGA GCAAATCATT CACACAATGA CTCAAGACCT GAGGGAGGCC 
AATGAGAAAC TGACCATGTC CGAGTCGCGG GCTGAAAACA TCCGTAAGGA ACGAGACATT 
CTGAAACAGG TGGAAAACAG ACTCAACCAG GAGAAAGAGT CTATCCTGAC TGAACAGCGG 
AGCCAGAACC TTCTGCTCAC CAACCTCAAA TCCATTCAGC TCACCATGGA GCGCTCTGAA 
ACTGAGACCA AACAGCGCTA CAATAATCAA ATCCAGCATC TGGAAAAGGA GATTATCCAG 
TTAAAGAAGA GGCTGGAGCA AGAAGTGGAA CAGAGACACG CACTGGAACG CAACCAAGAT 
ACTCAGCTGT TGGAGGCTAA GAAGAAGCTG GAGTCACAGA CCGCACTACA CCAGAAGACT 
AAAGAGCTGC TGAGATCTGC TGAACAGCAG GTGACCTCTT TGAGACAGCA ACTGAACAAC 
AATGAGAATC AGAGTGCAGC ACCTAAGCAG CCTGTCAGGA CATTACCCAG AGTTTCAGCT 
CCCAGCGTTC CTCAACAGGA GGCAGATGAT CTTCGTGCAC GTCTACAGCA GGTCGAGGAA 
CAGAACTCTG ACCTGAAAGA ACGCTTGAAG AACACCACCA CCAATGTAGA GCAGTATAGA 
TCTATGGTGC TCAGCTTGGA GGAGTCTGTG AACAAGGAGA AACAGTTGGC TGAGCAAGCA 
CGGACCTCCA CTGACAACCA ACTGAAGATG GCTCGGGAGT TGAATCAGCA GCTGGAAGCC 
CGTTTGGTGG AAGCGGAGAA GGAGAAGTTG GAGCTCCAGC AGGAGAAGAT GAAAGCTGTG 
GATAGTGTTG AAGAACGGGT GAAGGAGCTG AAAAGGAGCA TGACAAATAT GCAGACTGAG 
CTTCAGGAAG CTCTGCAGAG ATCTACAGCA GCAGTCACTC AGGAGCAGAA AGCCACACAG 
GACAGCAAAG TACAGGCTAA GCTGGCTGCA GAGGCTCAGA ATAAATACGA GCGAGAGCTC 
ATGCTGCATG CTGCAGATGT GGAAGCCCTG CAGGCCGCCA AGAAACAGGG ACAGCTTTTA 
ACACAAAACA TGAAGCAGCT CGAGGAGAAA GCTCAGAAAG CTGCATCTGA ACTGCATCAG 
TACCGCACAG ACTGGGTAAT GCAGGAGAAG AGACTAAAGG AAACGCTTTC TAAAGAGCAG 
AAGAGAGCCG AGGAGCTGCA GAAGCAGAAC ACTTTGCTCC ATGAGCAGAT GGAGAACTTG 
AGCAGCAAGA TCACTGCCTC AACCCAGCAG GAGACTGGAG AGAGCAGTCT GAACATCTCT 
CTGAACGAGG AGGGCAAGTC TCATGAGCAG ATACTGGACA TCCTCAGGTT TGTCCGGCGG 
GAGAAGGAGA TTGCAGAGAC CCGACTGGAA GTGGCCGAGG TGGAGACCCT TCGCTACAAA 
CAGCGCATGG AGTATCTGGA GTGCGAGCTG AAGGAACTAC AGGACAGCCT GAATGTAGAG 
AGAGAGAAGT TGCAGGTGAC AACAAAGACC TTGGTACAGC AGGAAGAGAC GATGAAGAGG 
ATGGAGAGCA TGAGTGCTCT TACAGATACC AACAAAATGC TGAAAAATGA GAAAAATCGA 
CTGGAGCATG AGCTGCAGCA GACTCAGGCC AAGATGCGTA AGCTCGAAGC AGACATCAAG 
CCTCTGCAGG AGGCCAATGG TGAGCTGAGT GAGAAGAGTG GCATGTTACA GGCTGAGAAG 
AGGCTTCTGG AGGAGGATGT CAAGCGCTTG AAGTCTCGCA CACAGCAACT CTTGAATCAG 
CAGAAGGACA GCGATCAGGA GGAAAGCAAG AAGCTTCATT CTGAGAGAGA GACTCATCTT 
AAACGCATCC AGCAGCTCAC AGAGGAGACT GGCAAACTGA AGAATGAGGT GGCCCGGAGC 
CAAGCTTCAA TCACCACGGC TCAGAGCCAA GTGCAGAACC TGCAAGACAG TCTGGGGAAG 
GTCACCACTG AACGAGACAG TCTGAAGAAG GAGCTGGAGG CAAAAACACT GGACATCCAG 
GAGAAAGTCA AAACCATCAC GCAGGTCAAG AAAATCGGCC GTCGCTACAA AACCCAGTAT 
GAAGAGCTCA AAGAACAGCA TGATAAGATG GTGGCAGAGG CTGCTTCTAA ACCAGCCCAG 
GAGCAGGAAG ACCGTCAGGC CTCAGTGCAG GAGATTCAGA ACCTGAAGAG CTCATTGAGT 
CAGACACAGA ATCGAACCTC AGAGCTGGAA ACTCAGCTGG ACTGCATACA GAAGACGTTG 
CAGGAGCGTG AAGCAGAGGT GAAGAGCCTA CAGGAACAGC TGACACAAGT GCAGCCTGAG 
CTCAGCCGTC TAAAAGCAGA ACTGCAGGAG AAGGGCAAAC TGGAGGAGCA GCTCCGACAG 
CAGATCGCTG ATAAAGAGGA GAAGACCAAG AAAGCCTTCT TGGGGGCCAA ACAGAAGCTC 
AACCAGCTCA ACAGTGCAAA GGAGCAGCTG GCGAAAGAAA ACGAGGAGCT GAAGCTGCAG 
CGAGAGGAGC TGGAGGTGAG GATGAGTGCA CTGAAGTCTC AATATGAAGG TCGACTTAGC 
CGACAAGAAC GAGAGCTGAG GGAACTTCGT GAACAGCAGG AGAGACATGG AGAACAGAGA 
GATGAAGCTC AGGAGCCTGG CCAGAGCAAG GTTCAAGAGG CACCGAGGTC ATCAGATGCT 
CGTCAGATCA CACTCAAGCC TACGCCTGCT GCTGACAGAG GAAGTGCAAG TCAGTCTGAA 
CCACCCACTG CCAACATCAA GCCCACGTTA GTTGCCGCCA CCCCTAGCAA GCCTTCACCA 
ATCGCAGGCA GCAAGTCCAC TCCTAAAGCA AGCATCCGTC CAATGATAAC GCCTGCTCCT 
GTTACCACAC CCACAGCCAC AGTCATGCCC ACCACACAGG TTGAAACGCA GGAAGCTCTA 
CAATCCACAG AGGGTCCGCT GGAGCATGTG ACTGTGTTTG GCAGCACCAG TGGCTCAGTG 
CGCTCCACCA GCCCAAACAT ACAGACCACA CAGCCAATCC TCAGCCTGCA GCAGAGCCAA 
GCCACTGCCT TCGTCCAGCC CACTCAGCAG CAGGCCAGCC AGGAACCAGC AACCAGCATT 
ATGGAAGCGG TGCATAGCTC TCAGATGGAG AGGCCCTCCA CCTCCACCGC TGTCTTTGGT 
ACAGTTTCTG CAACAACGGG TTCTTCGGTG GCCAAGCGAG TGCGAGAGGA GCATGAGGAG 
AGCTCCACTG AGGTGACGGA CACCACACAG GATGACTCTA CACATCCACC CATCATGAAG 
AAGCTCCGCA TTGTAAGAAG AGTGGGTCTG GAGGATGCTT CAGGTACTGA GGAGAGTAAT 
GATGCTATGG GTGAATCTTC AGGAGACGTT CAGCAGCCTC CTGATGCCAG TGAGGAAGCC 
TATCCCGTAT TAGGAGAGGG GGATGAAGAA TCATTGTCTG CCCCCATGGA TCAGGTGAGT 
GAGTCTCAAC CCTCTGAGTC TCAGATCAGT GGACAGGACT CATCAGAAGA GTACAAGCAT 
GATGTAATCG TCATCGAAAC AGACAGCGCC ACTGAGGAGG AGGAGGAAGA GGAAGAGGAG 
CAGGAAGAAC CTGGACAGTA TGAAGATGAT GATGAGGATG AGGACGATGA CGCAGAAGAT 
GAGGATGATG AAGATGGGGA TGGAGGAATG GCTGAAGCAG CAGAGGAGAG TAATGAAGGC 
AGTGGGAGTG CAGATGCCAA TGAGGCTTAT GAGGGGGATG AGGCAGAGCA GGGTGCCGGG 
GAAGCTGATC CAAGTGTTCA CAATGAAGAG AGTCAAGAGG CATCTGATTC AACCCAGAAG 
CAACCAGACT CTCAGAGCAG TGAAGCTAGT GTCAGCACCC TGGAGGCCTT CCCTCTTGAG 
CCTGTAAGAG AGCACCCGCC GCTGCCCAGC ACTACCACCA CCTCCCTCGC CCCGCGTCTG 
CCCCAATCCC CCCGTAGACC ACATCACACC CCTCCACCTC GTCTTAATAT CCACCCTGTC 
CCTGAACTTG GACCTCCTAT CATGCAGAGG CAGTCTGGAC AGAGCAGACG ACCATCAATG 
AGTCGCGCAC CGCAGCTTAC GCCCGGTATT GGAAGCATGC AACACTTCTT TGATGACGAT 
GACAGAATGG TTCCCAGCAC TCCCACTCTT GTGGTGCCAC ACCGCACAGA TGGCTTTGCG 
GAGGCCATTC ATTCCCCTCA GGTGGCTGGA GTTCCTCGCT TCCGTTTCGG CCCCCCAGAG 
GACATGATGC CTCAGGCCAG CTCTTCTCAT TCTGACCTGA GCCATCTGGC CACACATGGA 
GGACTGGGCA TGTATGAAAG TCCACTGTTC CTGCCCACTC ATGATGAGGA GTCTGGAGGC 
AGGAGTGTGC CCACCACCCC TCTACAAGTG GCCGCTCCAG TGACCGTGTT CACCGAAAGT 
CACCCGTCGG ATGTTTCGGA GACGGCCTCT CAGTCAGTGC CCATGGTAAG CACATCCACC 
GCCAGCCTGA GTGCACCAGG AGAGGCTGTG CCAGGAGATG ATGGAGATGA CGTGTTCGTG 
GCTGAGGCTG AGAGCGAAGG GGTGAGTAGT GAAGCGTGTC TAGAAAGCCA GTTGGAGCTA 
GATTCTGCAC AACCGGCTGA TGATGCCAGT CTTCCCTCCA CCAGTCAAGA GCCTACGTCT 
AGTTCTGCAG ACACTAGCAC CAGTAGCGGT CAGGCTAAAC CCGCAGGCCG TCTGCTTCCT 
CAGCACCATC TGCCCCGGAG AAACCAGCTG ATCCGGAGAG GTTTTCCTCG TGGAGGTCGG 
GGTCGAGGGC TCAGCAGAGG CTCCCCTTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPRA ( XM_009298497.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296772.1 Danio rerio translocated promoter region a, nuclear basket protein (tpra), transcript variant X1, mRNA.

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CloneID	ODa46084
Clone ID Related Accession (Same CDS sequence)	XM_009298497.3 , XM_009298497.2
Accession Version	XM_009298497.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	nucleoprotein TPR isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298497.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCAC TTCTTCAGCA AGCGCTGGAA AGAACCGAGG TCAACAAACT GCCGAAAGCC 
ATTCAGAACA AGCTCGAGAA GGTTCTGGCA GAGCAGCAGA CGGAAATCGA CTCGTTGATA 
TCACAGCATG AGCGGTATAA AGCAGACTGT GAGCAACAGT ACTTTAATCT CGAGAAGAAG 
CTTGCTGAGA GTCAGGAGCA GTTTCTATCC CAAAGTAAGG ACTACCACAA TATTAAGGAG 
GAAAACAACC GTCTTGTGGA AGAGCTTAAG AAGTTAAAAG AGATAGAAGA AGTGCAGGGA 
ACAACGCAAG AGAGTAAGCC TCCTAGAGCC AAATATGAAA TCGAGGCAGA AAACCGAGAG 
CTGTCACGAT TGCTTGAGAA GAGATCTCAA GAAGTGGAAA ACCTCAGTGA GGATCTCAAA 
CGTCTCAATG ACAAACTTGT GGAGACCAAC ACAATCAAGA TGGAGCTCCA GCTTAAACTA 
GATGAACTTC AGTCTTCTGA GGTTTCCATT CAGTATCGTG AGAAGCGGAT GGAACAAGAA 
AAAGAGCTTC TCCAGAACCA GAACACATGG CTGAACACAG AGCTGAAGAG TAAGACGGAT 
GAGCTGTACA CCGTATCCAG AGACAAAGGC AAAGAGAACC TGGAGCTTAG GTGTTCTTTA 
GAGAGCAAGA AGGAAGAGGT GACCAGACTT CAGGATCAAG TAAACACACT CAAGAAAGCA 
AATGACAGCA TGCAGAAGAG TACAGAGGAT CTGATGAGGA AGCTAAAAGA GGCTAAAGAA 
CAACGGGCCT CAATGGAAGA GAAGTACCGC AATGAGCTGA ATGCAAACCT CAAACTATGT 
AATTTGTACA AGGGTGCAGC ATCAGATTCT GAAGCCAAGA ACACTGAACT GAACAGAGCT 
GTGGAAGAGC TCAATAAACT GCTGAAGGAA GCAATGGAAG CCAATAAAGG AACCGAGAAG 
AAGCTGTCAG AGTTGCAGAG CGTAAGGGAG AAAGCAGAGT CAGATTTACG TGAGAAGGTT 
AGGCATCTGG AGAAGGAGCT GGAGAACTCC AACACAAGGC TCGCAGACTT TAAGAGGAGA 
GGTGTTCCAG CTCTCACAGA AGAGGAGCTG ACAAACCTTT CTCCAACTGC AGCGGCAGTG 
GCCAAGATCG TGAAGCCGGG CATGAAGCTA ATGGAGTTGT ACAATGCCTT TGTGGAGGCA 
CAAGACCAGC TGCACCTTGA GAAGTTGGAG AACAAGCGTG TGAACAAGGT CCTTGATGAG 
ATCGTGCTGG AGGTGGAAAC CAAAGCTCCC ATCCTGAAAC GCCAGAGAGA GGAATATGAG 
AGCATGCAGA AGTCTATGAC CAGTCTCTGT TATAAACTAG AGCAAGCTAT GAAGGAGGTC 
CATCGGCTAC AAAAAGAAAC GGATGAAGCC AATAAGAGAG CTTTAGTGCT TGAAAGAGAT 
AATCAGAAAT CAGAGAGACA GCTAGCCGAC ATGTCTGAGC AGGTGCGTGT GCTTCTGGTG 
GAGGTGGAGG AGGCACGTGG TAATCAGGTA GTGCGTGAGG ATGTGAGTTC TGCTGTGAGC 
AGTAGCTCTG AGGTCCAGGG TTCACGACAG GTGGCATTCC GCAGCATCCA AGAGCTTCAG 
CAGCAGAACC AGAACTTACT GGTCAAGATC AGAGACCTGG AAGAGCAGAG AGAAAAAGAC 
CAGAACCAGG CTAAGAGTGC AAAGCAAACA GAATTGGAAC AAAGTCTGGA GAAGGTTCAG 
AAGGAGCTGG AGCAGATCAA GGAACAGCAA AACCATCAGA AGCTGCTGGC TGAATCTGCC 
ATCCGCCAGA GAGACATGTA CCGTATCCTT TTGCAGACAG CCGGAGTCGA GCTTCCACCA 
CAGGGTTCAG ATGGTGGATC TCAATCCACT ACTCCCAGCA GGCCAGGACC CATGGCCACT 
AGATCCACAC CACTAAGGGC TGCTGCTGCA GAATCTTTCC AGGCAACCCA GTCTAAAGCA 
GCACTTAAAC AGATAAATGA TGCCTTTACT ACATATAAAA AGGAGAAGGC CGAAAATGAC 
AAGCTTCTAA ATGAGCAGAT TGAGAGGCTT CGTGGTCAAG TGTCTGACCT GCTCTCACAA 
AAGGCCAAAC TGTCCTCTCA GCTGGAGTTT GCCTCTAAAC GGTATGATAT GCTTCAGGAG 
AATGTGAATG GCTTTCGAAG AGAGACTGAA GCCCTGCGGG AGAGGAATCA AAAGATGTCT 
GCCACCCATC AGAGACATGA GCAAATCATT CACACAATGA CTCAAGACCT GAGGGAGGCC 
AATGAGAAAC TGACCATGTC CGAGTCGCGG GCTGAAAACA TCCGTAAGGA ACGAGACATT 
CTGAAACAGG TGGAAAACAG ACTCAACCAG GAGAAAGAGT CTATCCTGAC TGAACAGCGG 
AGCCAGAACC TTCTGCTCAC CAACCTCAAA TCCATTCAGC TCACCATGGA GCGCTCTGAA 
ACTGAGACCA AACAGCGCTA CAATAATCAA ATCCAGCATC TGGAAAAGGA GATTATCCAG 
TTAAAGAAGA GGCTGGAGCA AGAAGTGGAA CAGAGACACG CACTGGAACG CAACCAAGAT 
ACTCAGCTGT TGGAGGCTAA GAAGAAGCTG GAGTCACAGA CCGCACTACA CCAGAAGACT 
AAAGAGCTGC TGAGATCTGC TGAACAGCAG GTGACCTCTT TGAGACAGCA ACTGAACAAC 
AATGAGAATC AGAGTGCAGC ACCTAAGCAG CCTGTCAGGA CATTACCCAG AGTTTCAGCT 
CCCAGCGTTC CTCAACAGGA GGCAGATGAT CTTCGTGCAC GTCTACAGCA GGTCGAGGAA 
CAGAACTCTG ACCTGAAAGA ACGCTTGAAG AACACCACCA CCAATGTAGA GCAGTATAGA 
TCTATGGTGC TCAGCTTGGA GGAGTCTGTG AACAAGGAGA AACAGTTGGC TGAGCAAGCA 
CGGACCTCCA CTGACAACCA ACTGAAGATG GCTCGGGAGT TGAATCAGCA GCTGGAAGCC 
CGTTTGGTGG AAGCGGAGAA GGAGAAGTTG GAGCTCCAGC AGGAGAAGAT GAAAGCTGTG 
GATAGTGTTG AAGAACGGGT GAAGGAGCTG AAAAGGAGCA TGACAAATAT GCAGACTGAG 
CTTCAGGAAG CTCTGCAGAG ATCTACAGCA GCAGTCACTC AGGAGCAGAA AGCCACACAG 
GACAGCAAAG TACAGGCTAA GCTGGCTGCA GAGGCTCAGA ATAAATACGA GCGAGAGCTC 
ATGCTGCATG CTGCAGATGT GGAAGCCCTG CAGGCCGCCA AGAAACAGGG ACAGCTTTTA 
ACACAAAACA TGAAGCAGCT CGAGGAGAAA GCTCAGAAAG CTGCATCTGA ACTGCATCAG 
TACCGCACAG ACTGGGTAAT GCAGGAGAAG AGACTAAAGG AAACGCTTTC TAAAGAGCAG 
AAGAGAGCCG AGGAGCTGCA GAAGCAGAAC ACTTTGCTCC ATGAGCAGAT GGAGAACTTG 
AGCAGCAAGA TCACTGCCTC AACCCAGCAG GAGACTGGAG AGAGCAGTCT GAACATCTCT 
CTGAACGAGG AGGGCAAGTC TCATGAGCAG ATACTGGACA TCCTCAGGTT TGTCCGGCGG 
GAGAAGGAGA TTGCAGAGAC CCGACTGGAA GTGGCCGAGG TGGAGACCCT TCGCTACAAA 
CAGCGCATGG AGTATCTGGA GTGCGAGCTG AAGGAACTAC AGGACAGCCT GAATGTAGAG 
AGAGAGAAGT TGCAGGTGAC AACAAAGACC TTGGTACAGC AGGAAGAGAC GATGAAGAGG 
ATGGAGAGCA TGAGTGCTCT TACAGATACC AACAAAATGC TGAAAAATGA GAAAAATCGA 
CTGGAGCATG AGCTGCAGCA GACTCAGGCC AAGATGCGTA AGCTCGAAGC AGACATCAAG 
CCTCTGCAGG AGGCCAATGG TGAGCTGAGT GAGAAGAGTG GCATGTTACA GGCTGAGAAG 
AGGCTTCTGG AGGAGGATGT CAAGCGCTTG AAGTCTCGCA CACAGCAACT CTTGAATCAG 
CAGAAGGACA GCGATCAGGA GGAAAGCAAG AAGCTTCATT CTGAGAGAGA GACTCATCTT 
AAACGCATCC AGCAGCTCAC AGAGGAGACT GGCAAACTGA AGAATGAGGT GGCCCGGAGC 
CAAGCTTCAA TCACCACGGC TCAGAGCCAA GTGCAGAACC TGCAAGACAG TCTGGGGAAG 
GTCACCACTG AACGAGACAG TCTGAAGAAG GAGCTGGAGG CAAAAACACT GGACATCCAG 
GAGAAAGTCA AAACCATCAC GCAGGTCAAG AAAATCGGCC GTCGCTACAA AACCCAGTAT 
GAAGAGCTCA AAGAACAGCA TGATAAGATG GTGGCAGAGG CTGCTTCTAA ACCAGCCCAG 
GAGCAGGAAG ACCGTCAGGC CTCAGTGCAG GAGATTCAGA ACCTGAAGAG CTCATTGAGT 
CAGACACAGA ATCGAACCTC AGAGCTGGAA ACTCAGCTGG ACTGCATACA GAAGACGTTG 
CAGGAGCGTG AAGCAGAGGT GAAGAGCCTA CAGGAACAGC TGACACAAGT GCAGCCTGAG 
CTCAGCCGTC TAAAAGCAGA ACTGCAGGAG AAGGGCAAAC TGGAGGAGCA GCTCCGACAG 
CAGATCGCTG ATAAAGAGGA GAAGACCAAG AAAGCCTTCT TGGGGGCCAA ACAGAAGCTC 
AACCAGCTCA ACAGTGCAAA GGAGCAGCTG GCGAAAGAAA ACGAGGAGCT GAAGCTGCAG 
CGAGAGGAGC TGGAGGTGAG GATGAGTGCA CTGAAGTCTC AATATGAAGG TCGACTTAGC 
CGACAAGAAC GAGAGCTGAG GGAACTTCGT GAACAGCAGG AGAGACATGG AGAACAGAGA 
GATGAAGCTC AGGAGCCTGG CCAGAGCAAG GTTCAAGAGG CACCGAGGTC ATCAGATGCT 
CGTCAGATCA CACTCAAGCC TACGCCTGCT GCTGACAGAG GAAGTGCAAG TCAGTCTGAA 
CCACCCACTG CCAACATCAA GCCCACGTTA GTTGCCGCCA CCCCTAGCAA GCCTTCACCA 
ATCGCAGGCA GCAAGTCCAC TCCTAAAGCA AGCATCCGTC CAATGATAAC GCCTGCTCCT 
GTTACCACAC CCACAGCCAC AGTCATGCCC ACCACACAGG TTGAAACGCA GGAAGCTCTA 
CAATCCACAG AGGGTCCGCT GGAGCATGTG ACTGTGTTTG GCAGCACCAG TGGCTCAGTG 
CGCTCCACCA GCCCAAACAT ACAGACCACA CAGCCAATCC TCAGCCTGCA GCAGAGCCAA 
GCCACTGCCT TCGTCCAGCC CACTCAGCAG CAGGCCAGCC AGGAACCAGC AACCAGCATT 
ATGGAAGCGG TGCATAGCTC TCAGATGGAG AGGCCCTCCA CCTCCACCGC TGTCTTTGGT 
ACAGTTTCTG CAACAACGGG TTCTTCGGTG GCCAAGCGAG TGCGAGAGGA GCATGAGGAG 
AGCTCCACTG AGGTGACGGA CACCACACAG GATGACTCTA CACATCCACC CATCATGAAG 
AAGCTCCGCA TTGTAAGAAG AGTGGGTCTG GAGGATGCTT CAGGTACTGA GGAGAGTAAT 
GATGCTATGG GTGAATCTTC AGGAGACGTT CAGCAGCCTC CTGATGCCAG TGAGGAAGCC 
TATCCCGTAT TAGGAGAGGG GGATGAAGAA TCATTGTCTG CCCCCATGGA TCAGGTGAGT 
GAGTCTCAAC CCTCTGAGTC TCAGATCAGT GGACAGGACT CATCAGAAGA GTACAAGCAT 
GATGTAATCG TCATCGAAAC AGACAGCGCC ACTGAGGAGG AGGAGGAAGA GGAAGAGGAG 
CAGGAAGAAC CTGGACAGTA TGAAGATGAT GATGAGGATG AGGACGATGA CGCAGAAGAT 
GAGGATGATG AAGATGGGGA TGGAGGAATG GCTGAAGCAG CAGAGGAGAG TAATGAAGGC 
AGTGGGAGTG CAGATGCCAA TGAGGCTTAT GAGGGGGATG AGGCAGAGCA GGGTGCCGGG 
GAAGCTGATC CAAGTGTTCA CAATGAAGAG AGTCAAGAGG CATCTGATTC AACCCAGAAG 
CAACCAGACT CTCAGAGCAG TGAAGCTAGT GTCAGCACCC TGGAGGCCTT CCCTCTTGAG 
CCTGTAAGAG AGCACCCGCC GCTGCCCAGC ACTACCACCA CCTCCCTCGC CCCGCGTCTG 
CCCCAATCCC CCCGTAGACC ACATCACACC CCTCCACCTC GTCTTAATAT CCACCCTGTC 
CCTGAACTTG GACCTCCTAT CATGCAGAGG CAGTCTGGAC AGAGCAGACG ACCATCAATG 
AGTCGCGCAC CGCAGCTTAC GCCCGGTATT GGAAGCATGC AACACTTCTT TGATGACGAT 
GACAGAATGG TTCCCAGCAC TCCCACTCTT GTGGTGCCAC ACCGCACAGA TGGCTTTGCG 
GAGGCCATTC ATTCCCCTCA GGTGGCTGGA GTTCCTCGCT TCCGTTTCGG CCCCCCAGAG 
GACATGATGC CTCAGGCCAG CTCTTCTCAT TCTGACCTGA GCCATCTGGC CACACATGGA 
GGACTGGGCA TGTATGAAAG TCCACTGTTC CTGCCCACTC ATGATGAGGA GTCTGGAGGC 
AGGAGTGTGC CCACCACCCC TCTACAAGTG GCCGCTCCAG TGACCGTGTT CACCGAAAGT 
CACCCGTCGG ATGTTTCGGA GACGGCCTCT CAGTCAGTGC CCATGGTAAG CACATCCACC 
GCCAGCCTGA GTGCACCAGG AGAGGCTGTG CCAGGAGATG ATGGAGATGA CGTGTTCGTG 
GCTGAGGCTG AGAGCGAAGG GGTGAGTAGT GAAGCGTGTC TAGAAAGCCA GTTGGAGCTA 
GATTCTGCAC AACCGGCTGA TGATGCCAGT CTTCCCTCCA CCAGTCAAGA GCCTACGTCT 
AGTTCTGCAG ACACTAGCAC CAGTAGCGGT CAGGCTAAAC CCGCAGGCCG TCTGCTTCCT 
CAGCACCATC TGCCCCGGAG AAACCAGCTG ATCCGGAGAG GTTTTCCTCG TGGAGGTCGG 
GGTCGAGGGC TCAGCAGAGG CTCCCCTTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296772.1
CDS	57..7109
Translation	MAALLQQALERTEVNKLPKAIQNKLEKVLAEQQTEIDSLISQHERYKADCEQQYFNLEKKLAESQEQFLSQSKDYHNIKEENNRLVEELKKLKEIEEVQGTTQESKPPRAKYEIEAENRELSRLLEKRSQEVENLSEDLKRLNDKLVETNTIKMELQLKLDELQSSEVSIQYREKRMEQEKELLQNQNTWLNTELKSKTDELYTVSRDKGKENLELRCSLESKKEEVTRLQDQVNTLKKANDSMQKSTEDLMRKLKEAKEQRASMEEKYRNELNANLKLCNLYKGAASDSEAKNTELNRAVEELNKLLKEAMEANKGTEKKLSELQSVREKAESDLREKVRHLEKELENSNTRLADFKRRGVPALTEEELTNLSPTAAAVAKIVKPGMKLMELYNAFVEAQDQLHLEKLENKRVNKVLDEIVLEVETKAPILKRQREEYESMQKSMTSLCYKLEQAMKEVHRLQKETDEANKRALVLERDNQKSERQLADMSEQVRVLLVEVEEARGNQVVREDVSSAVSSSSEVQGSRQVAFRSIQELQQQNQNLLVKIRDLEEQREKDQNQAKSAKQTELEQSLEKVQKELEQIKEQQNHQKLLAESAIRQRDMYRILLQTAGVELPPQGSDGGSQSTTPSRPGPMATRSTPLRAAAAESFQATQSKAALKQINDAFTTYKKEKAENDKLLNEQIERLRGQVSDLLSQKAKLSSQLEFASKRYDMLQENVNGFRRETEALRERNQKMSATHQRHEQIIHTMTQDLREANEKLTMSESRAENIRKERDILKQVENRLNQEKESILTEQRSQNLLLTNLKSIQLTMERSETETKQRYNNQIQHLEKEIIQLKKRLEQEVEQRHALERNQDTQLLEAKKKLESQTALHQKTKELLRSAEQQVTSLRQQLNNNENQSAAPKQPVRTLPRVSAPSVPQQEADDLRARLQQVEEQNSDLKERLKNTTTNVEQYRSMVLSLEESVNKEKQLAEQARTSTDNQLKMARELNQQLEARLVEAEKEKLELQQEKMKAVDSVEERVKELKRSMTNMQTELQEALQRSTAAVTQEQKATQDSKVQAKLAAEAQNKYERELMLHAADVEALQAAKKQGQLLTQNMKQLEEKAQKAASELHQYRTDWVMQEKRLKETLSKEQKRAEELQKQNTLLHEQMENLSSKITASTQQETGESSLNISLNEEGKSHEQILDILRFVRREKEIAETRLEVAEVETLRYKQRMEYLECELKELQDSLNVEREKLQVTTKTLVQQEETMKRMESMSALTDTNKMLKNEKNRLEHELQQTQAKMRKLEADIKPLQEANGELSEKSGMLQAEKRLLEEDVKRLKSRTQQLLNQQKDSDQEESKKLHSERETHLKRIQQLTEETGKLKNEVARSQASITTAQSQVQNLQDSLGKVTTERDSLKKELEAKTLDIQEKVKTITQVKKIGRRYKTQYEELKEQHDKMVAEAASKPAQEQEDRQASVQEIQNLKSSLSQTQNRTSELETQLDCIQKTLQEREAEVKSLQEQLTQVQPELSRLKAELQEKGKLEEQLRQQIADKEEKTKKAFLGAKQKLNQLNSAKEQLAKENEELKLQREELEVRMSALKSQYEGRLSRQERELRELREQQERHGEQRDEAQEPGQSKVQEAPRSSDARQITLKPTPAADRGSASQSEPPTANIKPTLVAATPSKPSPIAGSKSTPKASIRPMITPAPVTTPTATVMPTTQVETQEALQSTEGPLEHVTVFGSTSGSVRSTSPNIQTTQPILSLQQSQATAFVQPTQQQASQEPATSIMEAVHSSQMERPSTSTAVFGTVSATTGSSVAKRVREEHEESSTEVTDTTQDDSTHPPIMKKLRIVRRVGLEDASGTEESNDAMGESSGDVQQPPDASEEAYPVLGEGDEESLSAPMDQVSESQPSESQISGQDSSEEYKHDVIVIETDSATEEEEEEEEEQEEPGQYEDDDEDEDDDAEDEDDEDGDGGMAEAAEESNEGSGSADANEAYEGDEAEQGAGEADPSVHNEESQEASDSTQKQPDSQSSEASVSTLEAFPLEPVREHPPLPSTTTTSLAPRLPQSPRRPHHTPPPRLNIHPVPELGPPIMQRQSGQSRRPSMSRAPQLTPGIGSMQHFFDDDDRMVPSTPTLVVPHRTDGFAEAIHSPQVAGVPRFRFGPPEDMMPQASSSHSDLSHLATHGGLGMYESPLFLPTHDEESGGRSVPTTPLQVAAPVTVFTESHPSDVSETASQSVPMVSTSTASLSAPGEAVPGDDGDDVFVAEAESEGVSSEACLESQLELDSAQPADDASLPSTSQEPTSSSADTSTSSGQAKPAGRLLPQHHLPRRNQLIRRGFPRGGRGRGLSRGSPF

Target ORF information:

RefSeq Version	XM_009298497.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio translocated promoter region a, nuclear basket protein (tpra), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298497.2

ORF Insert Sequence:

ATGGCGGCAC TTCTTCAGCA AGCGCTGGAA AGAACCGAGG TCAACAAACT GCCGAAAGCC 
ATTCAGAACA AGCTCGAGAA GGTTCTGGCA GAGCAGCAGA CGGAAATCGA CTCGTTGATA 
TCACAGCATG AGCGGTATAA AGCAGACTGT GAGCAACAGT ACTTTAATCT CGAGAAGAAG 
CTTGCTGAGA GTCAGGAGCA GTTTCTATCC CAAAGTAAGG ACTACCACAA TATTAAGGAG 
GAAAACAACC GTCTTGTGGA AGAGCTTAAG AAGTTAAAAG AGATAGAAGA AGTGCAGGGA 
ACAACGCAAG AGAGTAAGCC TCCTAGAGCC AAATATGAAA TCGAGGCAGA AAACCGAGAG 
CTGTCACGAT TGCTTGAGAA GAGATCTCAA GAAGTGGAAA ACCTCAGTGA GGATCTCAAA 
CGTCTCAATG ACAAACTTGT GGAGACCAAC ACAATCAAGA TGGAGCTCCA GCTTAAACTA 
GATGAACTTC AGTCTTCTGA GGTTTCCATT CAGTATCGTG AGAAGCGGAT GGAACAAGAA 
AAAGAGCTTC TCCAGAACCA GAACACATGG CTGAACACAG AGCTGAAGAG TAAGACGGAT 
GAGCTGTACA CCGTATCCAG AGACAAAGGC AAAGAGAACC TGGAGCTTAG GTGTTCTTTA 
GAGAGCAAGA AGGAAGAGGT GACCAGACTT CAGGATCAAG TAAACACACT CAAGAAAGCA 
AATGACAGCA TGCAGAAGAG TACAGAGGAT CTGATGAGGA AGCTAAAAGA GGCTAAAGAA 
CAACGGGCCT CAATGGAAGA GAAGTACCGC AATGAGCTGA ATGCAAACCT CAAACTATGT 
AATTTGTACA AGGGTGCAGC ATCAGATTCT GAAGCCAAGA ACACTGAACT GAACAGAGCT 
GTGGAAGAGC TCAATAAACT GCTGAAGGAA GCAATGGAAG CCAATAAAGG AACCGAGAAG 
AAGCTGTCAG AGTTGCAGAG CGTAAGGGAG AAAGCAGAGT CAGATTTACG TGAGAAGGTT 
AGGCATCTGG AGAAGGAGCT GGAGAACTCC AACACAAGGC TCGCAGACTT TAAGAGGAGA 
GGTGTTCCAG CTCTCACAGA AGAGGAGCTG ACAAACCTTT CTCCAACTGC AGCGGCAGTG 
GCCAAGATCG TGAAGCCGGG CATGAAGCTA ATGGAGTTGT ACAATGCCTT TGTGGAGGCA 
CAAGACCAGC TGCACCTTGA GAAGTTGGAG AACAAGCGTG TGAACAAGGT CCTTGATGAG 
ATCGTGCTGG AGGTGGAAAC CAAAGCTCCC ATCCTGAAAC GCCAGAGAGA GGAATATGAG 
AGCATGCAGA AGTCTATGAC CAGTCTCTGT TATAAACTAG AGCAAGCTAT GAAGGAGGTC 
CATCGGCTAC AAAAAGAAAC GGATGAAGCC AATAAGAGAG CTTTAGTGCT TGAAAGAGAT 
AATCAGAAAT CAGAGAGACA GCTAGCCGAC ATGTCTGAGC AGGTGCGTGT GCTTCTGGTG 
GAGGTGGAGG AGGCACGTGG TAATCAGGTA GTGCGTGAGG ATGTGAGTTC TGCTGTGAGC 
AGTAGCTCTG AGGTCCAGGG TTCACGACAG GTGGCATTCC GCAGCATCCA AGAGCTTCAG 
CAGCAGAACC AGAACTTACT GGTCAAGATC AGAGACCTGG AAGAGCAGAG AGAAAAAGAC 
CAGAACCAGG CTAAGAGTGC AAAGCAAACA GAATTGGAAC AAAGTCTGGA GAAGGTTCAG 
AAGGAGCTGG AGCAGATCAA GGAACAGCAA AACCATCAGA AGCTGCTGGC TGAATCTGCC 
ATCCGCCAGA GAGACATGTA CCGTATCCTT TTGCAGACAG CCGGAGTCGA GCTTCCACCA 
CAGGGTTCAG ATGGTGGATC TCAATCCACT ACTCCCAGCA GGCCAGGACC CATGGCCACT 
AGATCCACAC CACTAAGGGC TGCTGCTGCA GAATCTTTCC AGGCAACCCA GTCTAAAGCA 
GCACTTAAAC AGATAAATGA TGCCTTTACT ACATATAAAA AGGAGAAGGC CGAAAATGAC 
AAGCTTCTAA ATGAGCAGAT TGAGAGGCTT CGTGGTCAAG TGTCTGACCT GCTCTCACAA 
AAGGCCAAAC TGTCCTCTCA GCTGGAGTTT GCCTCTAAAC GGTATGATAT GCTTCAGGAG 
AATGTGAATG GCTTTCGAAG AGAGACTGAA GCCCTGCGGG AGAGGAATCA AAAGATGTCT 
GCCACCCATC AGAGACATGA GCAAATCATT CACACAATGA CTCAAGACCT GAGGGAGGCC 
AATGAGAAAC TGACCATGTC CGAGTCGCGG GCTGAAAACA TCCGTAAGGA ACGAGACATT 
CTGAAACAGG TGGAAAACAG ACTCAACCAG GAGAAAGAGT CTATCCTGAC TGAACAGCGG 
AGCCAGAACC TTCTGCTCAC CAACCTCAAA TCCATTCAGC TCACCATGGA GCGCTCTGAA 
ACTGAGACCA AACAGCGCTA CAATAATCAA ATCCAGCATC TGGAAAAGGA GATTATCCAG 
TTAAAGAAGA GGCTGGAGCA AGAAGTGGAA CAGAGACACG CACTGGAACG CAACCAAGAT 
ACTCAGCTGT TGGAGGCTAA GAAGAAGCTG GAGTCACAGA CCGCACTACA CCAGAAGACT 
AAAGAGCTGC TGAGATCTGC TGAACAGCAG GTGACCTCTT TGAGACAGCA ACTGAACAAC 
AATGAGAATC AGAGTGCAGC ACCTAAGCAG CCTGTCAGGA CATTACCCAG AGTTTCAGCT 
CCCAGCGTTC CTCAACAGGA GGCAGATGAT CTTCGTGCAC GTCTACAGCA GGTCGAGGAA 
CAGAACTCTG ACCTGAAAGA ACGCTTGAAG AACACCACCA CCAATGTAGA GCAGTATAGA 
TCTATGGTGC TCAGCTTGGA GGAGTCTGTG AACAAGGAGA AACAGTTGGC TGAGCAAGCA 
CGGACCTCCA CTGACAACCA ACTGAAGATG GCTCGGGAGT TGAATCAGCA GCTGGAAGCC 
CGTTTGGTGG AAGCGGAGAA GGAGAAGTTG GAGCTCCAGC AGGAGAAGAT GAAAGCTGTG 
GATAGTGTTG AAGAACGGGT GAAGGAGCTG AAAAGGAGCA TGACAAATAT GCAGACTGAG 
CTTCAGGAAG CTCTGCAGAG ATCTACAGCA GCAGTCACTC AGGAGCAGAA AGCCACACAG 
GACAGCAAAG TACAGGCTAA GCTGGCTGCA GAGGCTCAGA ATAAATACGA GCGAGAGCTC 
ATGCTGCATG CTGCAGATGT GGAAGCCCTG CAGGCCGCCA AGAAACAGGG ACAGCTTTTA 
ACACAAAACA TGAAGCAGCT CGAGGAGAAA GCTCAGAAAG CTGCATCTGA ACTGCATCAG 
TACCGCACAG ACTGGGTAAT GCAGGAGAAG AGACTAAAGG AAACGCTTTC TAAAGAGCAG 
AAGAGAGCCG AGGAGCTGCA GAAGCAGAAC ACTTTGCTCC ATGAGCAGAT GGAGAACTTG 
AGCAGCAAGA TCACTGCCTC AACCCAGCAG GAGACTGGAG AGAGCAGTCT GAACATCTCT 
CTGAACGAGG AGGGCAAGTC TCATGAGCAG ATACTGGACA TCCTCAGGTT TGTCCGGCGG 
GAGAAGGAGA TTGCAGAGAC CCGACTGGAA GTGGCCGAGG TGGAGACCCT TCGCTACAAA 
CAGCGCATGG AGTATCTGGA GTGCGAGCTG AAGGAACTAC AGGACAGCCT GAATGTAGAG 
AGAGAGAAGT TGCAGGTGAC AACAAAGACC TTGGTACAGC AGGAAGAGAC GATGAAGAGG 
ATGGAGAGCA TGAGTGCTCT TACAGATACC AACAAAATGC TGAAAAATGA GAAAAATCGA 
CTGGAGCATG AGCTGCAGCA GACTCAGGCC AAGATGCGTA AGCTCGAAGC AGACATCAAG 
CCTCTGCAGG AGGCCAATGG TGAGCTGAGT GAGAAGAGTG GCATGTTACA GGCTGAGAAG 
AGGCTTCTGG AGGAGGATGT CAAGCGCTTG AAGTCTCGCA CACAGCAACT CTTGAATCAG 
CAGAAGGACA GCGATCAGGA GGAAAGCAAG AAGCTTCATT CTGAGAGAGA GACTCATCTT 
AAACGCATCC AGCAGCTCAC AGAGGAGACT GGCAAACTGA AGAATGAGGT GGCCCGGAGC 
CAAGCTTCAA TCACCACGGC TCAGAGCCAA GTGCAGAACC TGCAAGACAG TCTGGGGAAG 
GTCACCACTG AACGAGACAG TCTGAAGAAG GAGCTGGAGG CAAAAACACT GGACATCCAG 
GAGAAAGTCA AAACCATCAC GCAGGTCAAG AAAATCGGCC GTCGCTACAA AACCCAGTAT 
GAAGAGCTCA AAGAACAGCA TGATAAGATG GTGGCAGAGG CTGCTTCTAA ACCAGCCCAG 
GAGCAGGAAG ACCGTCAGGC CTCAGTGCAG GAGATTCAGA ACCTGAAGAG CTCATTGAGT 
CAGACACAGA ATCGAACCTC AGAGCTGGAA ACTCAGCTGG ACTGCATACA GAAGACGTTG 
CAGGAGCGTG AAGCAGAGGT GAAGAGCCTA CAGGAACAGC TGACACAAGT GCAGCCTGAG 
CTCAGCCGTC TAAAAGCAGA ACTGCAGGAG AAGGGCAAAC TGGAGGAGCA GCTCCGACAG 
CAGATCGCTG ATAAAGAGGA GAAGACCAAG AAAGCCTTCT TGGGGGCCAA ACAGAAGCTC 
AACCAGCTCA ACAGTGCAAA GGAGCAGCTG GCGAAAGAAA ACGAGGAGCT GAAGCTGCAG 
CGAGAGGAGC TGGAGGTGAG GATGAGTGCA CTGAAGTCTC AATATGAAGG TCGACTTAGC 
CGACAAGAAC GAGAGCTGAG GGAACTTCGT GAACAGCAGG AGAGACATGG AGAACAGAGA 
GATGAAGCTC AGGAGCCTGG CCAGAGCAAG GTTCAAGAGG CACCGAGGTC ATCAGATGCT 
CGTCAGATCA CACTCAAGCC TACGCCTGCT GCTGACAGAG GAAGTGCAAG TCAGTCTGAA 
CCACCCACTG CCAACATCAA GCCCACGTTA GTTGCCGCCA CCCCTAGCAA GCCTTCACCA 
ATCGCAGGCA GCAAGTCCAC TCCTAAAGCA AGCATCCGTC CAATGATAAC GCCTGCTCCT 
GTTACCACAC CCACAGCCAC AGTCATGCCC ACCACACAGG TTGAAACGCA GGAAGCTCTA 
CAATCCACAG AGGGTCCGCT GGAGCATGTG ACTGTGTTTG GCAGCACCAG TGGCTCAGTG 
CGCTCCACCA GCCCAAACAT ACAGACCACA CAGCCAATCC TCAGCCTGCA GCAGAGCCAA 
GCCACTGCCT TCGTCCAGCC CACTCAGCAG CAGGCCAGCC AGGAACCAGC AACCAGCATT 
ATGGAAGCGG TGCATAGCTC TCAGATGGAG AGGCCCTCCA CCTCCACCGC TGTCTTTGGT 
ACAGTTTCTG CAACAACGGG TTCTTCGGTG GCCAAGCGAG TGCGAGAGGA GCATGAGGAG 
AGCTCCACTG AGGTGACGGA CACCACACAG GATGACTCTA CACATCCACC CATCATGAAG 
AAGCTCCGCA TTGTAAGAAG AGTGGGTCTG GAGGATGCTT CAGGTACTGA GGAGAGTAAT 
GATGCTATGG GTGAATCTTC AGGAGACGTT CAGCAGCCTC CTGATGCCAG TGAGGAAGCC 
TATCCCGTAT TAGGAGAGGG GGATGAAGAA TCATTGTCTG CCCCCATGGA TCAGGTGAGT 
GAGTCTCAAC CCTCTGAGTC TCAGATCAGT GGACAGGACT CATCAGAAGA GTACAAGCAT 
GATGTAATCG TCATCGAAAC AGACAGCGCC ACTGAGGAGG AGGAGGAAGA GGAAGAGGAG 
CAGGAAGAAC CTGGACAGTA TGAAGATGAT GATGAGGATG AGGACGATGA CGCAGAAGAT 
GAGGATGATG AAGATGGGGA TGGAGGAATG GCTGAAGCAG CAGAGGAGAG TAATGAAGGC 
AGTGGGAGTG CAGATGCCAA TGAGGCTTAT GAGGGGGATG AGGCAGAGCA GGGTGCCGGG 
GAAGCTGATC CAAGTGTTCA CAATGAAGAG AGTCAAGAGG CATCTGATTC AACCCAGAAG 
CAACCAGACT CTCAGAGCAG TGAAGCTAGT GTCAGCACCC TGGAGGCCTT CCCTCTTGAG 
CCTGTAAGAG AGCACCCGCC GCTGCCCAGC ACTACCACCA CCTCCCTCGC CCCGCGTCTG 
CCCCAATCCC CCCGTAGACC ACATCACACC CCTCCACCTC GTCTTAATAT CCACCCTGTC 
CCTGAACTTG GACCTCCTAT CATGCAGAGG CAGTCTGGAC AGAGCAGACG ACCATCAATG 
AGTCGCGCAC CGCAGCTTAC GCCCGGTATT GGAAGCATGC AACACTTCTT TGATGACGAT 
GACAGAATGG TTCCCAGCAC TCCCACTCTT GTGGTGCCAC ACCGCACAGA TGGCTTTGCG 
GAGGCCATTC ATTCCCCTCA GGTGGCTGGA GTTCCTCGCT TCCGTTTCGG CCCCCCAGAG 
GACATGATGC CTCAGGCCAG CTCTTCTCAT TCTGACCTGA GCCATCTGGC CACACATGGA 
GGACTGGGCA TGTATGAAAG TCCACTGTTC CTGCCCACTC ATGATGAGGA GTCTGGAGGC 
AGGAGTGTGC CCACCACCCC TCTACAAGTG GCCGCTCCAG TGACCGTGTT CACCGAAAGT 
CACCCGTCGG ATGTTTCGGA GACGGCCTCT CAGTCAGTGC CCATGGTAAG CACATCCACC 
GCCAGCCTGA GTGCACCAGG AGAGGCTGTG CCAGGAGATG ATGGAGATGA CGTGTTCGTG 
GCTGAGGCTG AGAGCGAAGG GGTGAGTAGT GAAGCGTGTC TAGAAAGCCA GTTGGAGCTA 
GATTCTGCAC AACCGGCTGA TGATGCCAGT CTTCCCTCCA CCAGTCAAGA GCCTACGTCT 
AGTTCTGCAG ACACTAGCAC CAGTAGCGGT CAGGCTAAAC CCGCAGGCCG TCTGCTTCCT 
CAGCACCATC TGCCCCGGAG AAACCAGCTG ATCCGGAGAG GTTTTCCTCG TGGAGGTCGG 
GGTCGAGGGC TCAGCAGAGG CTCCCCTTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPRA ( XM_009298498.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296773.1 Danio rerio translocated promoter region a, nuclear basket protein (tpra), transcript variant X2, mRNA.

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CloneID	ODa46085
Clone ID Related Accession (Same CDS sequence)	XM_009298498.3 , XM_009298498.2
Accession Version	XM_009298498.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	nucleoprotein TPR isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298498.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCAC TTCTTCAGCA AGCGCTGGAA AGAACCGAGG TCAACAAACT GCCGAAAGCC 
ATTCAGAACA AGCTCGAGAA GGTTCTGGCA GAGCAGCAGA CGGAAATCGA CTCGTTGATA 
TCACAGCATG AGCGGTATAA AGCAGACTGT GAGCAACAGT ACTTTAATCT CGAGAAGAAG 
CTTGCTGAGA GTCAGGAGCA GTTTCTATCC CAAAGTAAGG ACTACCACAA TATTAAGGAG 
GAAAACAACC GTCTTGTGGA AGAGCTTAAG AAGTTAAAAG AGATAGAAGA AGTGCAGGGA 
ACAACGCAAG AGAGTAAGCC TCCTAGAGCC AAATATGAAA TCGAGGCAGA AAACCGAGAG 
CTGTCACGAT TGCTTGAGAA GAGATCTCAA GAAGTGGAAA ACCTCAGTGA GGATCTCAAA 
CGTCTCAATG ACAAACTTGT GGAGACCAAC ACAATCAAGA TGGAGCTCCA GCTTAAACTA 
GATGAACTTC AGTCTTCTGA GGTTTCCATT CAGTATCGTG AGAAGCGGAT GGAACAAGAA 
AAAGAGCTTC TCCAGAACCA GAACACATGG CTGAACACAG AGCTGAAGAG TAAGACGGAT 
GAGCTGTACA CCGTATCCAG AGACAAAGGC AAAGAGAACC TGGAGCTTAG GTGTTCTTTA 
GAGAGCAAGA AGGAAGAGGT GACCAGACTT CAGGATCAAG TAAACACACT CAAGAAAGCA 
AATGACAGCA TGCAGAAGAG TACAGAGGAT CTGATGAGGA AGCTAAAAGA GGCTAAAGAA 
CAACGGGCCT CAATGGAAGA GAAGTACCGC AATGAGCTGA ATGCAAACCT CAAACTATGT 
AATTTGTACA AGGGTGCAGC ATCAGATTCT GAAGCCAAGA ACACTGAACT GAACAGAGCT 
GTGGAAGAGC TCAATAAACT GCTGAAGGAA GCAATGGAAG CCAATAAAGG AACCGAGAAG 
AAGCTGTCAG AGTTGCAGAG CGTAAGGGAG AAAGCAGAGT CAGATTTACG TGAGAAGGTT 
AGGCATCTGG AGAAGGAGCT GGAGAACTCC AACACAAGGC TCGCAGACTT TAAGAGGAGA 
GGTGTTCCAG CTCTCACAGA AGAGGAGCTG ACAAACCTTT CTCCAACTGC AGCGGCAGTG 
GCCAAGATCG TGAAGCCGGG CATGAAGCTA ATGGAGTTGT ACAATGCCTT TGTGGAGGCA 
CAAGACCAGC TGCACCTTGA GAAGTTGGAG AACAAGCGTG TGAACAAGGT CCTTGATGAG 
ATCGTGCTGG AGGTGGAAAC CAAAGCTCCC ATCCTGAAAC GCCAGAGAGA GGAATATGAG 
AGCATGCAGA AGTCTATGAC CAGTCTCTGT TATAAACTAG AGCAAGCTAT GAAGGAGGTC 
CATCGGCTAC AAAAAGAAAC GGATGAAGCC AATAAGAGAG CTTTAGTGCT TGAAAGAGAT 
AATCAGAAAT CAGAGAGACA GCTAGCCGAC ATGTCTGAGC AGGTGCGTGT GCTTCTGGTG 
GAGGTGGAGG AGGCACGTGG TAATCAGGTA GTGCGTGAGG ATGTGAGTTC TGCTGTGAGC 
AGTAGCTCTG AGGTCCAGGG TTCACGACAG GTGGCATTCC GCAGCATCCA AGAGCTTCAG 
CAGCAGAACC AGAACTTACT GGTCAAGATC AGAGACCTGG AAGAGCAGAG AGAAAAAGAC 
CAGAACCAGG CTAAGAGTGC AAAGCAAACA GAATTGGAAC AAAGTCTGGA GAAGGTTCAG 
AAGGAGCTGG AGCAGATCAA GGAACAGCAA AACCATCAGA AGCTGCTGGC TGAATCTGCC 
ATCCGCCAGA GAGACATGTA CCGTATCCTT TTGCAGACAG CCGGAGTCGA GCTTCCACCA 
CAGGGTTCAG ATGGTGGATC TCAATCCACT ACTCCCAGCA GGCCAGGACC CATGGCCACT 
AGATCCACAC CACTAAGGGC TGCTGCTGCA GAATCTTTCC AGGCAACCCA GTCTAAAGCA 
GCACTTAAAC AGATAAATGA TGCCTTTACT ACATATAAAA AGGAGAAGGC CGAAAATGAC 
AAGCTTCTAA ATGAGCAGAT TGAGAGGCTT CGTGGTCAAG TGTCTGACCT GCTCTCACAA 
AAGGCCAAAC TGTCCTCTCA GCTGGAGTTT GCCTCTAAAC GGTATGATAT GCTTCAGGAG 
AATGTGAATG GCTTTCGAAG AGAGACTGAA GCCCTGCGGG AGAGGAATCA AAAGATGTCT 
GCCACCCATC AGAGACATGA GCAAATCATT CACACAATGA CTCAAGACCT GAGGGAGGCC 
AATGAGAAAC TGACCATGTC CGAGTCGCGG GCTGAAAACA TCCGTAAGGA ACGAGACATT 
CTGAAACAGG TGGAAAACAG ACTCAACCAG GAGAAAGAGT CTATCCTGAC TGAACAGCGG 
AGCCAGAACC TTCTGCTCAC CAACCTCAAA TCCATTCAGC TCACCATGGA GCGCTCTGAA 
ACTGAGACCA AACAGCGCTA CAATAATCAA ATCCAGCATC TGGAAAAGGA GATTATCCAG 
TTAAAGAAGA GGCTGGAGCA AGAAGTGGAA CAGAGACACG CACTGGAACG CAACCAAGAT 
ACTCAGCTGT TGGAGGCTAA GAAGAAGCTG GAGTCACAGA CCGCACTACA CCAGAAGACT 
AAAGAGCTGC TGAGATCTGC TGAACAGCAG GTGACCTCTT TGAGACAGCA ACTGAACAAC 
AATGAGAATC AGAGTGCAGC ACCTAAGCAG CCTGTCAGGA CATTACCCAG AGTTTCAGCT 
CCCAGCGTTC CTCAACAGGA GGCAGATGAT CTTCGTGCAC GTCTACAGCA GGTCGAGGAA 
CAGAACTCTG ACCTGAAAGA ACGCTTGAAG AACACCACCA CCAATGTAGA GCAGTATAGA 
TCTATGGTGC TCAGCTTGGA GGAGTCTGTG AACAAGGAGA AACAGTTGGC TGAGCAAGCA 
CGGACCTCCA CTGACAACCA ACTGAAGATG GCTCGGGAGT TGAATCAGCA GCTGGAAGCC 
CGTTTGGTGG AAGCGGAGAA GGAGAAGTTG GAGCTCCAGC AGGAGAAGAT GAAAGCTGTG 
GATAGTGTTG AAGAACGGGT GAAGGAGCTG AAAAGGAGCA TGACAAATAT GCAGACTGAG 
CTTCAGGAAG CTCTGCAGAG ATCTACAGCA GCAGTCACTC AGGAGCAGAA AGCCACACAG 
GACAGCAAAG TACAGGCTAA GCTGGCTGCA GAGGCTCAGA ATAAATACGA GCGAGAGCTC 
ATGCTGCATG CTGCAGATGT GGAAGCCCTG CAGGCCGCCA AGAAACAGGG ACAGCTTTTA 
ACACAAAACA TGAAGCAGCT CGAGGAGAAA GCTCAGAAAG CTGCATCTGA ACTGCATCAG 
TACCGCACAG ACTGGGTAAT GCAGGAGAAG AGACTAAAGG AAACGCTTTC TAAAGAGCAG 
AAGAGAGCCG AGGAGCTGCA GAAGCAGAAC ACTTTGCTCC ATGAGCAGAT GGAGAACTTG 
AGCAGCAAGA TCACTGCCTC AACCCAGCAG GAGACTGGAG AGAGCAGTCT GAACATCTCT 
CTGAACGAGG AGGGCAAGTC TCATGAGCAG ATACTGGACA TCCTCAGGTT TGTCCGGCGG 
GAGAAGGAGA TTGCAGAGAC CCGACTGGAA GTGGCCGAGG TGGAGACCCT TCGCTACAAA 
CAGCGCATGG AGTATCTGGA GTGCGAGCTG AAGGAACTAC AGGACAGCCT GAATGTAGAG 
AGAGAGAAGT TGCAGGTGAC AACAAAGACC TTGGTACAGC AGGAAGAGAC GATGAAGAGG 
ATGGAGAGCA TGAGTGCTCT TACAGATACC AACAAAATGC TGAAAAATGA GAAAAATCGA 
CTGGAGCATG AGCTGCAGCA GACTCAGGCC AAGATGCGTA AGCTCGAAGC AGACATCAAG 
CCTCTGCAGG AGGCCAATGG TGAGCTGAGT GAGAAGAGTG GCATGTTACA GGCTGAGAAG 
AGGCTTCTGG AGGAGGATGT CAAGCGCTTG AAGTCTCGCA CACAGCAACT CTTGAATCAG 
CAGAAGGACA GCGATCAGGA GGAAAGCAAG AAGCTTCATT CTGAGAGAGA GACTCATCTT 
AAACGCATCC AGCAGCTCAC AGAGGAGACT GGCAAACTGA AGAATGAGGT GGCCCGGAGC 
CAAGCTTCAA TCACCACGGC TCAGAGCCAA GTGCAGAACC TGCAAGACAG TCTGGGGAAG 
GTCACCACTG AACGAGACAG TCTGAAGAAG GAGCTGGAGG CAAAAACACT GGACATCCAG 
GAGAAAGTCA AAACCATCAC GCAGGTCAAG AAAATCGGCC GTCGCTACAA AACCCAGTAT 
GAAGAGCTCA AAGAACAGCA TGATAAGATG GTGGCAGAGG CTGCTTCTAA ACCAGCCCAG 
GAGCAGGAAG ACCGTCAGGC CTCAGTGCAG GAGATTCAGA ACCTGAAGAG CTCATTGAGT 
CAGACACAGA ATCGAACCTC AGAGCTGGAA ACTCAGCTGG ACTGCATACA GAAGACGTTG 
CAGGAGCGTG AAGCAGAGGT GAAGAGCCTA CAGGAACAGC TGACACAAGT GCAGCCTGAG 
CTCAGCCGTC TAAAAGCAGA ACTGCAGGAG AAGGGCAAAC TGGAGGAGCA GCTCCGACAG 
CAGATCGCTG ATAAAGAGGA GAAGACCAAG AAAGCCTTCT TGGGGGCCAA ACAGAAGCTC 
AACCAGCTCA ACAGTGCAAA GGAGCAGCTG GCGAAAGAAA ACGAGGAGCT GAAGCTGCAG 
CGAGAGGAGC TGGAGGTGAG GATGAGTGCA CTGAAGTCTC AATATGAAGG TCGACTTAGC 
CGACAAGAAC GAGAGCTGAG GGAACTTCGT GAACAGCAGG AGAGACATGG AGAACAGAGA 
GATGAAGCTC AGGAGCCTGG CCAGAGCAAG GTTCAAGAGG CACCGAGGTC ATCAGATGCT 
CGTCAGATCA CACTCAAGCC TACGCCTGCT GCTGACAGAG GAAGTGCAAG TCAGTCTGAA 
CCACCCACTG CCAACATCAA GCCCACGTTA GTTGCCGCCA CCCCTAGCAA GCCTTCACCA 
ATCGCAGGCA GCAAGTCCAC TCCTAAAGCA AGCATCCGTC CAATGATAAC GCCTGCTCCT 
GTTACCACAC CCACAGCCAC AGTCATGCCC ACCACACAGG TTGAAACGCA GGAAGCTCTA 
CAATCCACAG AGGGTCCGCT GGAGCATGTG ACTGTGTTTG GCAGCACCAG TGGCTCAGTG 
CGCTCCACCA GCCCAAACAT ACAGACCACA CAGCCAATCC TCAGCCTGCA GCAGAGCCAA 
GCCACTGCCT TCGTCCAGCC CACTCAGCAG CAGGCCAGCC AGGAACCAGC AACCAGCATT 
ATGGAAGCGG TGCATAGCTC TCAGATGGAG AGGCCCTCCA CCTCCACCGC TGTCTTTGGT 
ACAGTTTCTG CAACAACGGG TTCTTCGGTG GCCAAGCGAG TGCGAGAGGA GCATGAGGAG 
AGCTCCACTG AGGTGACGGA CACCACACAG GATGACTCTA CACATCCACC CATCATGAAG 
AAGCTCCGCA TTGTAAGAAG AGTGGGTCTG GAGGATGCTT CAGGTACTGA GGAGAGTAAT 
GATGCTATGG GTGAATCTTC AGGAGACGTT CAGCAGCCTC CTGATGCCAG TGAGGAAGCC 
TATCCCGTAT TAGGAGAGGG GGATGAAGAA TCATTGTCTG CCCCCATGGA TCAGGTGAGT 
GAGTCTCAAC CCTCTGAGTC TCAGATCAGT GGACAGGACT CATCAGAAGA GTACAAGCAT 
GATGTAATCG TCATCGAAAC AGACAGCGCC ACTGAGGAGG AGGAGGAAGA GGAAGAGGAG 
CAGGAAGAAC CTGGACAGTA TGAAGATGAT GATGAGGATG AGGACGATGA CGCAGAAGAT 
GAGGATGATG AAGATGGGGA TGGAGGAATG GCTGAAGCAG CAGAGGAGAG TAATGAAGGC 
AGTGGGAGTG CAGATGCCAA TGAGGCTTAT GAGGGGGATG AGGCAGAGGG TGCCGGGGAA 
GCTGATCCAA GTGTTCACAA TGAAGAGAGT CAAGAGGCAT CTGATTCAAC CCAGAAGCAA 
CCAGACTCTC AGAGCAGTGA AGCTAGTGTC AGCACCCTGG AGGCCTTCCC TCTTGAGCCT 
GTAAGAGAGC ACCCGCCGCT GCCCAGCACT ACCACCACCT CCCTCGCCCC GCGTCTGCCC 
CAATCCCCCC GTAGACCACA TCACACCCCT CCACCTCGTC TTAATATCCA CCCTGTCCCT 
GAACTTGGAC CTCCTATCAT GCAGAGGCAG TCTGGACAGA GCAGACGACC ATCAATGAGT 
CGCGCACCGC AGCTTACGCC CGGTATTGGA AGCATGCAAC ACTTCTTTGA TGACGATGAC 
AGAATGGTTC CCAGCACTCC CACTCTTGTG GTGCCACACC GCACAGATGG CTTTGCGGAG 
GCCATTCATT CCCCTCAGGT GGCTGGAGTT CCTCGCTTCC GTTTCGGCCC CCCAGAGGAC 
ATGATGCCTC AGGCCAGCTC TTCTCATTCT GACCTGAGCC ATCTGGCCAC ACATGGAGGA 
CTGGGCATGT ATGAAAGTCC ACTGTTCCTG CCCACTCATG ATGAGGAGTC TGGAGGCAGG 
AGTGTGCCCA CCACCCCTCT ACAAGTGGCC GCTCCAGTGA CCGTGTTCAC CGAAAGTCAC 
CCGTCGGATG TTTCGGAGAC GGCCTCTCAG TCAGTGCCCA TGGTAAGCAC ATCCACCGCC 
AGCCTGAGTG CACCAGGAGA GGCTGTGCCA GGAGATGATG GAGATGACGT GTTCGTGGCT 
GAGGCTGAGA GCGAAGGGGT GAGTAGTGAA GCGTGTCTAG AAAGCCAGTT GGAGCTAGAT 
TCTGCACAAC CGGCTGATGA TGCCAGTCTT CCCTCCACCA GTCAAGAGCC TACGTCTAGT 
TCTGCAGACA CTAGCACCAG TAGCGGTCAG GCTAAACCCG CAGGCCGTCT GCTTCCTCAG 
CACCATCTGC CCCGGAGAAA CCAGCTGATC CGGAGAGGTT TTCCTCGTGG AGGTCGGGGT 
CGAGGGCTCA GCAGAGGCTC CCCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296773.1
CDS	23..7072
Translation	MAALLQQALERTEVNKLPKAIQNKLEKVLAEQQTEIDSLISQHERYKADCEQQYFNLEKKLAESQEQFLSQSKDYHNIKEENNRLVEELKKLKEIEEVQGTTQESKPPRAKYEIEAENRELSRLLEKRSQEVENLSEDLKRLNDKLVETNTIKMELQLKLDELQSSEVSIQYREKRMEQEKELLQNQNTWLNTELKSKTDELYTVSRDKGKENLELRCSLESKKEEVTRLQDQVNTLKKANDSMQKSTEDLMRKLKEAKEQRASMEEKYRNELNANLKLCNLYKGAASDSEAKNTELNRAVEELNKLLKEAMEANKGTEKKLSELQSVREKAESDLREKVRHLEKELENSNTRLADFKRRGVPALTEEELTNLSPTAAAVAKIVKPGMKLMELYNAFVEAQDQLHLEKLENKRVNKVLDEIVLEVETKAPILKRQREEYESMQKSMTSLCYKLEQAMKEVHRLQKETDEANKRALVLERDNQKSERQLADMSEQVRVLLVEVEEARGNQVVREDVSSAVSSSSEVQGSRQVAFRSIQELQQQNQNLLVKIRDLEEQREKDQNQAKSAKQTELEQSLEKVQKELEQIKEQQNHQKLLAESAIRQRDMYRILLQTAGVELPPQGSDGGSQSTTPSRPGPMATRSTPLRAAAAESFQATQSKAALKQINDAFTTYKKEKAENDKLLNEQIERLRGQVSDLLSQKAKLSSQLEFASKRYDMLQENVNGFRRETEALRERNQKMSATHQRHEQIIHTMTQDLREANEKLTMSESRAENIRKERDILKQVENRLNQEKESILTEQRSQNLLLTNLKSIQLTMERSETETKQRYNNQIQHLEKEIIQLKKRLEQEVEQRHALERNQDTQLLEAKKKLESQTALHQKTKELLRSAEQQVTSLRQQLNNNENQSAAPKQPVRTLPRVSAPSVPQQEADDLRARLQQVEEQNSDLKERLKNTTTNVEQYRSMVLSLEESVNKEKQLAEQARTSTDNQLKMARELNQQLEARLVEAEKEKLELQQEKMKAVDSVEERVKELKRSMTNMQTELQEALQRSTAAVTQEQKATQDSKVQAKLAAEAQNKYERELMLHAADVEALQAAKKQGQLLTQNMKQLEEKAQKAASELHQYRTDWVMQEKRLKETLSKEQKRAEELQKQNTLLHEQMENLSSKITASTQQETGESSLNISLNEEGKSHEQILDILRFVRREKEIAETRLEVAEVETLRYKQRMEYLECELKELQDSLNVEREKLQVTTKTLVQQEETMKRMESMSALTDTNKMLKNEKNRLEHELQQTQAKMRKLEADIKPLQEANGELSEKSGMLQAEKRLLEEDVKRLKSRTQQLLNQQKDSDQEESKKLHSERETHLKRIQQLTEETGKLKNEVARSQASITTAQSQVQNLQDSLGKVTTERDSLKKELEAKTLDIQEKVKTITQVKKIGRRYKTQYEELKEQHDKMVAEAASKPAQEQEDRQASVQEIQNLKSSLSQTQNRTSELETQLDCIQKTLQEREAEVKSLQEQLTQVQPELSRLKAELQEKGKLEEQLRQQIADKEEKTKKAFLGAKQKLNQLNSAKEQLAKENEELKLQREELEVRMSALKSQYEGRLSRQERELRELREQQERHGEQRDEAQEPGQSKVQEAPRSSDARQITLKPTPAADRGSASQSEPPTANIKPTLVAATPSKPSPIAGSKSTPKASIRPMITPAPVTTPTATVMPTTQVETQEALQSTEGPLEHVTVFGSTSGSVRSTSPNIQTTQPILSLQQSQATAFVQPTQQQASQEPATSIMEAVHSSQMERPSTSTAVFGTVSATTGSSVAKRVREEHEESSTEVTDTTQDDSTHPPIMKKLRIVRRVGLEDASGTEESNDAMGESSGDVQQPPDASEEAYPVLGEGDEESLSAPMDQVSESQPSESQISGQDSSEEYKHDVIVIETDSATEEEEEEEEEQEEPGQYEDDDEDEDDDAEDEDDEDGDGGMAEAAEESNEGSGSADANEAYEGDEAEGAGEADPSVHNEESQEASDSTQKQPDSQSSEASVSTLEAFPLEPVREHPPLPSTTTTSLAPRLPQSPRRPHHTPPPRLNIHPVPELGPPIMQRQSGQSRRPSMSRAPQLTPGIGSMQHFFDDDDRMVPSTPTLVVPHRTDGFAEAIHSPQVAGVPRFRFGPPEDMMPQASSSHSDLSHLATHGGLGMYESPLFLPTHDEESGGRSVPTTPLQVAAPVTVFTESHPSDVSETASQSVPMVSTSTASLSAPGEAVPGDDGDDVFVAEAESEGVSSEACLESQLELDSAQPADDASLPSTSQEPTSSSADTSTSSGQAKPAGRLLPQHHLPRRNQLIRRGFPRGGRGRGLSRGSPF

Target ORF information:

RefSeq Version	XM_009298498.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio translocated promoter region a, nuclear basket protein (tpra), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298498.3

ORF Insert Sequence:

ATGGCGGCAC TTCTTCAGCA AGCGCTGGAA AGAACCGAGG TCAACAAACT GCCGAAAGCC 
ATTCAGAACA AGCTCGAGAA GGTTCTGGCA GAGCAGCAGA CGGAAATCGA CTCGTTGATA 
TCACAGCATG AGCGGTATAA AGCAGACTGT GAGCAACAGT ACTTTAATCT CGAGAAGAAG 
CTTGCTGAGA GTCAGGAGCA GTTTCTATCC CAAAGTAAGG ACTACCACAA TATTAAGGAG 
GAAAACAACC GTCTTGTGGA AGAGCTTAAG AAGTTAAAAG AGATAGAAGA AGTGCAGGGA 
ACAACGCAAG AGAGTAAGCC TCCTAGAGCC AAATATGAAA TCGAGGCAGA AAACCGAGAG 
CTGTCACGAT TGCTTGAGAA GAGATCTCAA GAAGTGGAAA ACCTCAGTGA GGATCTCAAA 
CGTCTCAATG ACAAACTTGT GGAGACCAAC ACAATCAAGA TGGAGCTCCA GCTTAAACTA 
GATGAACTTC AGTCTTCTGA GGTTTCCATT CAGTATCGTG AGAAGCGGAT GGAACAAGAA 
AAAGAGCTTC TCCAGAACCA GAACACATGG CTGAACACAG AGCTGAAGAG TAAGACGGAT 
GAGCTGTACA CCGTATCCAG AGACAAAGGC AAAGAGAACC TGGAGCTTAG GTGTTCTTTA 
GAGAGCAAGA AGGAAGAGGT GACCAGACTT CAGGATCAAG TAAACACACT CAAGAAAGCA 
AATGACAGCA TGCAGAAGAG TACAGAGGAT CTGATGAGGA AGCTAAAAGA GGCTAAAGAA 
CAACGGGCCT CAATGGAAGA GAAGTACCGC AATGAGCTGA ATGCAAACCT CAAACTATGT 
AATTTGTACA AGGGTGCAGC ATCAGATTCT GAAGCCAAGA ACACTGAACT GAACAGAGCT 
GTGGAAGAGC TCAATAAACT GCTGAAGGAA GCAATGGAAG CCAATAAAGG AACCGAGAAG 
AAGCTGTCAG AGTTGCAGAG CGTAAGGGAG AAAGCAGAGT CAGATTTACG TGAGAAGGTT 
AGGCATCTGG AGAAGGAGCT GGAGAACTCC AACACAAGGC TCGCAGACTT TAAGAGGAGA 
GGTGTTCCAG CTCTCACAGA AGAGGAGCTG ACAAACCTTT CTCCAACTGC AGCGGCAGTG 
GCCAAGATCG TGAAGCCGGG CATGAAGCTA ATGGAGTTGT ACAATGCCTT TGTGGAGGCA 
CAAGACCAGC TGCACCTTGA GAAGTTGGAG AACAAGCGTG TGAACAAGGT CCTTGATGAG 
ATCGTGCTGG AGGTGGAAAC CAAAGCTCCC ATCCTGAAAC GCCAGAGAGA GGAATATGAG 
AGCATGCAGA AGTCTATGAC CAGTCTCTGT TATAAACTAG AGCAAGCTAT GAAGGAGGTC 
CATCGGCTAC AAAAAGAAAC GGATGAAGCC AATAAGAGAG CTTTAGTGCT TGAAAGAGAT 
AATCAGAAAT CAGAGAGACA GCTAGCCGAC ATGTCTGAGC AGGTGCGTGT GCTTCTGGTG 
GAGGTGGAGG AGGCACGTGG TAATCAGGTA GTGCGTGAGG ATGTGAGTTC TGCTGTGAGC 
AGTAGCTCTG AGGTCCAGGG TTCACGACAG GTGGCATTCC GCAGCATCCA AGAGCTTCAG 
CAGCAGAACC AGAACTTACT GGTCAAGATC AGAGACCTGG AAGAGCAGAG AGAAAAAGAC 
CAGAACCAGG CTAAGAGTGC AAAGCAAACA GAATTGGAAC AAAGTCTGGA GAAGGTTCAG 
AAGGAGCTGG AGCAGATCAA GGAACAGCAA AACCATCAGA AGCTGCTGGC TGAATCTGCC 
ATCCGCCAGA GAGACATGTA CCGTATCCTT TTGCAGACAG CCGGAGTCGA GCTTCCACCA 
CAGGGTTCAG ATGGTGGATC TCAATCCACT ACTCCCAGCA GGCCAGGACC CATGGCCACT 
AGATCCACAC CACTAAGGGC TGCTGCTGCA GAATCTTTCC AGGCAACCCA GTCTAAAGCA 
GCACTTAAAC AGATAAATGA TGCCTTTACT ACATATAAAA AGGAGAAGGC CGAAAATGAC 
AAGCTTCTAA ATGAGCAGAT TGAGAGGCTT CGTGGTCAAG TGTCTGACCT GCTCTCACAA 
AAGGCCAAAC TGTCCTCTCA GCTGGAGTTT GCCTCTAAAC GGTATGATAT GCTTCAGGAG 
AATGTGAATG GCTTTCGAAG AGAGACTGAA GCCCTGCGGG AGAGGAATCA AAAGATGTCT 
GCCACCCATC AGAGACATGA GCAAATCATT CACACAATGA CTCAAGACCT GAGGGAGGCC 
AATGAGAAAC TGACCATGTC CGAGTCGCGG GCTGAAAACA TCCGTAAGGA ACGAGACATT 
CTGAAACAGG TGGAAAACAG ACTCAACCAG GAGAAAGAGT CTATCCTGAC TGAACAGCGG 
AGCCAGAACC TTCTGCTCAC CAACCTCAAA TCCATTCAGC TCACCATGGA GCGCTCTGAA 
ACTGAGACCA AACAGCGCTA CAATAATCAA ATCCAGCATC TGGAAAAGGA GATTATCCAG 
TTAAAGAAGA GGCTGGAGCA AGAAGTGGAA CAGAGACACG CACTGGAACG CAACCAAGAT 
ACTCAGCTGT TGGAGGCTAA GAAGAAGCTG GAGTCACAGA CCGCACTACA CCAGAAGACT 
AAAGAGCTGC TGAGATCTGC TGAACAGCAG GTGACCTCTT TGAGACAGCA ACTGAACAAC 
AATGAGAATC AGAGTGCAGC ACCTAAGCAG CCTGTCAGGA CATTACCCAG AGTTTCAGCT 
CCCAGCGTTC CTCAACAGGA GGCAGATGAT CTTCGTGCAC GTCTACAGCA GGTCGAGGAA 
CAGAACTCTG ACCTGAAAGA ACGCTTGAAG AACACCACCA CCAATGTAGA GCAGTATAGA 
TCTATGGTGC TCAGCTTGGA GGAGTCTGTG AACAAGGAGA AACAGTTGGC TGAGCAAGCA 
CGGACCTCCA CTGACAACCA ACTGAAGATG GCTCGGGAGT TGAATCAGCA GCTGGAAGCC 
CGTTTGGTGG AAGCGGAGAA GGAGAAGTTG GAGCTCCAGC AGGAGAAGAT GAAAGCTGTG 
GATAGTGTTG AAGAACGGGT GAAGGAGCTG AAAAGGAGCA TGACAAATAT GCAGACTGAG 
CTTCAGGAAG CTCTGCAGAG ATCTACAGCA GCAGTCACTC AGGAGCAGAA AGCCACACAG 
GACAGCAAAG TACAGGCTAA GCTGGCTGCA GAGGCTCAGA ATAAATACGA GCGAGAGCTC 
ATGCTGCATG CTGCAGATGT GGAAGCCCTG CAGGCCGCCA AGAAACAGGG ACAGCTTTTA 
ACACAAAACA TGAAGCAGCT CGAGGAGAAA GCTCAGAAAG CTGCATCTGA ACTGCATCAG 
TACCGCACAG ACTGGGTAAT GCAGGAGAAG AGACTAAAGG AAACGCTTTC TAAAGAGCAG 
AAGAGAGCCG AGGAGCTGCA GAAGCAGAAC ACTTTGCTCC ATGAGCAGAT GGAGAACTTG 
AGCAGCAAGA TCACTGCCTC AACCCAGCAG GAGACTGGAG AGAGCAGTCT GAACATCTCT 
CTGAACGAGG AGGGCAAGTC TCATGAGCAG ATACTGGACA TCCTCAGGTT TGTCCGGCGG 
GAGAAGGAGA TTGCAGAGAC CCGACTGGAA GTGGCCGAGG TGGAGACCCT TCGCTACAAA 
CAGCGCATGG AGTATCTGGA GTGCGAGCTG AAGGAACTAC AGGACAGCCT GAATGTAGAG 
AGAGAGAAGT TGCAGGTGAC AACAAAGACC TTGGTACAGC AGGAAGAGAC GATGAAGAGG 
ATGGAGAGCA TGAGTGCTCT TACAGATACC AACAAAATGC TGAAAAATGA GAAAAATCGA 
CTGGAGCATG AGCTGCAGCA GACTCAGGCC AAGATGCGTA AGCTCGAAGC AGACATCAAG 
CCTCTGCAGG AGGCCAATGG TGAGCTGAGT GAGAAGAGTG GCATGTTACA GGCTGAGAAG 
AGGCTTCTGG AGGAGGATGT CAAGCGCTTG AAGTCTCGCA CACAGCAACT CTTGAATCAG 
CAGAAGGACA GCGATCAGGA GGAAAGCAAG AAGCTTCATT CTGAGAGAGA GACTCATCTT 
AAACGCATCC AGCAGCTCAC AGAGGAGACT GGCAAACTGA AGAATGAGGT GGCCCGGAGC 
CAAGCTTCAA TCACCACGGC TCAGAGCCAA GTGCAGAACC TGCAAGACAG TCTGGGGAAG 
GTCACCACTG AACGAGACAG TCTGAAGAAG GAGCTGGAGG CAAAAACACT GGACATCCAG 
GAGAAAGTCA AAACCATCAC GCAGGTCAAG AAAATCGGCC GTCGCTACAA AACCCAGTAT 
GAAGAGCTCA AAGAACAGCA TGATAAGATG GTGGCAGAGG CTGCTTCTAA ACCAGCCCAG 
GAGCAGGAAG ACCGTCAGGC CTCAGTGCAG GAGATTCAGA ACCTGAAGAG CTCATTGAGT 
CAGACACAGA ATCGAACCTC AGAGCTGGAA ACTCAGCTGG ACTGCATACA GAAGACGTTG 
CAGGAGCGTG AAGCAGAGGT GAAGAGCCTA CAGGAACAGC TGACACAAGT GCAGCCTGAG 
CTCAGCCGTC TAAAAGCAGA ACTGCAGGAG AAGGGCAAAC TGGAGGAGCA GCTCCGACAG 
CAGATCGCTG ATAAAGAGGA GAAGACCAAG AAAGCCTTCT TGGGGGCCAA ACAGAAGCTC 
AACCAGCTCA ACAGTGCAAA GGAGCAGCTG GCGAAAGAAA ACGAGGAGCT GAAGCTGCAG 
CGAGAGGAGC TGGAGGTGAG GATGAGTGCA CTGAAGTCTC AATATGAAGG TCGACTTAGC 
CGACAAGAAC GAGAGCTGAG GGAACTTCGT GAACAGCAGG AGAGACATGG AGAACAGAGA 
GATGAAGCTC AGGAGCCTGG CCAGAGCAAG GTTCAAGAGG CACCGAGGTC ATCAGATGCT 
CGTCAGATCA CACTCAAGCC TACGCCTGCT GCTGACAGAG GAAGTGCAAG TCAGTCTGAA 
CCACCCACTG CCAACATCAA GCCCACGTTA GTTGCCGCCA CCCCTAGCAA GCCTTCACCA 
ATCGCAGGCA GCAAGTCCAC TCCTAAAGCA AGCATCCGTC CAATGATAAC GCCTGCTCCT 
GTTACCACAC CCACAGCCAC AGTCATGCCC ACCACACAGG TTGAAACGCA GGAAGCTCTA 
CAATCCACAG AGGGTCCGCT GGAGCATGTG ACTGTGTTTG GCAGCACCAG TGGCTCAGTG 
CGCTCCACCA GCCCAAACAT ACAGACCACA CAGCCAATCC TCAGCCTGCA GCAGAGCCAA 
GCCACTGCCT TCGTCCAGCC CACTCAGCAG CAGGCCAGCC AGGAACCAGC AACCAGCATT 
ATGGAAGCGG TGCATAGCTC TCAGATGGAG AGGCCCTCCA CCTCCACCGC TGTCTTTGGT 
ACAGTTTCTG CAACAACGGG TTCTTCGGTG GCCAAGCGAG TGCGAGAGGA GCATGAGGAG 
AGCTCCACTG AGGTGACGGA CACCACACAG GATGACTCTA CACATCCACC CATCATGAAG 
AAGCTCCGCA TTGTAAGAAG AGTGGGTCTG GAGGATGCTT CAGGTACTGA GGAGAGTAAT 
GATGCTATGG GTGAATCTTC AGGAGACGTT CAGCAGCCTC CTGATGCCAG TGAGGAAGCC 
TATCCCGTAT TAGGAGAGGG GGATGAAGAA TCATTGTCTG CCCCCATGGA TCAGGTGAGT 
GAGTCTCAAC CCTCTGAGTC TCAGATCAGT GGACAGGACT CATCAGAAGA GTACAAGCAT 
GATGTAATCG TCATCGAAAC AGACAGCGCC ACTGAGGAGG AGGAGGAAGA GGAAGAGGAG 
CAGGAAGAAC CTGGACAGTA TGAAGATGAT GATGAGGATG AGGACGATGA CGCAGAAGAT 
GAGGATGATG AAGATGGGGA TGGAGGAATG GCTGAAGCAG CAGAGGAGAG TAATGAAGGC 
AGTGGGAGTG CAGATGCCAA TGAGGCTTAT GAGGGGGATG AGGCAGAGGG TGCCGGGGAA 
GCTGATCCAA GTGTTCACAA TGAAGAGAGT CAAGAGGCAT CTGATTCAAC CCAGAAGCAA 
CCAGACTCTC AGAGCAGTGA AGCTAGTGTC AGCACCCTGG AGGCCTTCCC TCTTGAGCCT 
GTAAGAGAGC ACCCGCCGCT GCCCAGCACT ACCACCACCT CCCTCGCCCC GCGTCTGCCC 
CAATCCCCCC GTAGACCACA TCACACCCCT CCACCTCGTC TTAATATCCA CCCTGTCCCT 
GAACTTGGAC CTCCTATCAT GCAGAGGCAG TCTGGACAGA GCAGACGACC ATCAATGAGT 
CGCGCACCGC AGCTTACGCC CGGTATTGGA AGCATGCAAC ACTTCTTTGA TGACGATGAC 
AGAATGGTTC CCAGCACTCC CACTCTTGTG GTGCCACACC GCACAGATGG CTTTGCGGAG 
GCCATTCATT CCCCTCAGGT GGCTGGAGTT CCTCGCTTCC GTTTCGGCCC CCCAGAGGAC 
ATGATGCCTC AGGCCAGCTC TTCTCATTCT GACCTGAGCC ATCTGGCCAC ACATGGAGGA 
CTGGGCATGT ATGAAAGTCC ACTGTTCCTG CCCACTCATG ATGAGGAGTC TGGAGGCAGG 
AGTGTGCCCA CCACCCCTCT ACAAGTGGCC GCTCCAGTGA CCGTGTTCAC CGAAAGTCAC 
CCGTCGGATG TTTCGGAGAC GGCCTCTCAG TCAGTGCCCA TGGTAAGCAC ATCCACCGCC 
AGCCTGAGTG CACCAGGAGA GGCTGTGCCA GGAGATGATG GAGATGACGT GTTCGTGGCT 
GAGGCTGAGA GCGAAGGGGT GAGTAGTGAA GCGTGTCTAG AAAGCCAGTT GGAGCTAGAT 
TCTGCACAAC CGGCTGATGA TGCCAGTCTT CCCTCCACCA GTCAAGAGCC TACGTCTAGT 
TCTGCAGACA CTAGCACCAG TAGCGGTCAG GCTAAACCCG CAGGCCGTCT GCTTCCTCAG 
CACCATCTGC CCCGGAGAAA CCAGCTGATC CGGAGAGGTT TTCCTCGTGG AGGTCGGGGT 
CGAGGGCTCA GCAGAGGCTC CCCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPRA ( XM_009298498.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296773.1 Danio rerio translocated promoter region a, nuclear basket protein (tpra), transcript variant X2, mRNA.

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CloneID	ODa46085
Clone ID Related Accession (Same CDS sequence)	XM_009298498.3 , XM_009298498.2
Accession Version	XM_009298498.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	nucleoprotein TPR isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298498.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCAC TTCTTCAGCA AGCGCTGGAA AGAACCGAGG TCAACAAACT GCCGAAAGCC 
ATTCAGAACA AGCTCGAGAA GGTTCTGGCA GAGCAGCAGA CGGAAATCGA CTCGTTGATA 
TCACAGCATG AGCGGTATAA AGCAGACTGT GAGCAACAGT ACTTTAATCT CGAGAAGAAG 
CTTGCTGAGA GTCAGGAGCA GTTTCTATCC CAAAGTAAGG ACTACCACAA TATTAAGGAG 
GAAAACAACC GTCTTGTGGA AGAGCTTAAG AAGTTAAAAG AGATAGAAGA AGTGCAGGGA 
ACAACGCAAG AGAGTAAGCC TCCTAGAGCC AAATATGAAA TCGAGGCAGA AAACCGAGAG 
CTGTCACGAT TGCTTGAGAA GAGATCTCAA GAAGTGGAAA ACCTCAGTGA GGATCTCAAA 
CGTCTCAATG ACAAACTTGT GGAGACCAAC ACAATCAAGA TGGAGCTCCA GCTTAAACTA 
GATGAACTTC AGTCTTCTGA GGTTTCCATT CAGTATCGTG AGAAGCGGAT GGAACAAGAA 
AAAGAGCTTC TCCAGAACCA GAACACATGG CTGAACACAG AGCTGAAGAG TAAGACGGAT 
GAGCTGTACA CCGTATCCAG AGACAAAGGC AAAGAGAACC TGGAGCTTAG GTGTTCTTTA 
GAGAGCAAGA AGGAAGAGGT GACCAGACTT CAGGATCAAG TAAACACACT CAAGAAAGCA 
AATGACAGCA TGCAGAAGAG TACAGAGGAT CTGATGAGGA AGCTAAAAGA GGCTAAAGAA 
CAACGGGCCT CAATGGAAGA GAAGTACCGC AATGAGCTGA ATGCAAACCT CAAACTATGT 
AATTTGTACA AGGGTGCAGC ATCAGATTCT GAAGCCAAGA ACACTGAACT GAACAGAGCT 
GTGGAAGAGC TCAATAAACT GCTGAAGGAA GCAATGGAAG CCAATAAAGG AACCGAGAAG 
AAGCTGTCAG AGTTGCAGAG CGTAAGGGAG AAAGCAGAGT CAGATTTACG TGAGAAGGTT 
AGGCATCTGG AGAAGGAGCT GGAGAACTCC AACACAAGGC TCGCAGACTT TAAGAGGAGA 
GGTGTTCCAG CTCTCACAGA AGAGGAGCTG ACAAACCTTT CTCCAACTGC AGCGGCAGTG 
GCCAAGATCG TGAAGCCGGG CATGAAGCTA ATGGAGTTGT ACAATGCCTT TGTGGAGGCA 
CAAGACCAGC TGCACCTTGA GAAGTTGGAG AACAAGCGTG TGAACAAGGT CCTTGATGAG 
ATCGTGCTGG AGGTGGAAAC CAAAGCTCCC ATCCTGAAAC GCCAGAGAGA GGAATATGAG 
AGCATGCAGA AGTCTATGAC CAGTCTCTGT TATAAACTAG AGCAAGCTAT GAAGGAGGTC 
CATCGGCTAC AAAAAGAAAC GGATGAAGCC AATAAGAGAG CTTTAGTGCT TGAAAGAGAT 
AATCAGAAAT CAGAGAGACA GCTAGCCGAC ATGTCTGAGC AGGTGCGTGT GCTTCTGGTG 
GAGGTGGAGG AGGCACGTGG TAATCAGGTA GTGCGTGAGG ATGTGAGTTC TGCTGTGAGC 
AGTAGCTCTG AGGTCCAGGG TTCACGACAG GTGGCATTCC GCAGCATCCA AGAGCTTCAG 
CAGCAGAACC AGAACTTACT GGTCAAGATC AGAGACCTGG AAGAGCAGAG AGAAAAAGAC 
CAGAACCAGG CTAAGAGTGC AAAGCAAACA GAATTGGAAC AAAGTCTGGA GAAGGTTCAG 
AAGGAGCTGG AGCAGATCAA GGAACAGCAA AACCATCAGA AGCTGCTGGC TGAATCTGCC 
ATCCGCCAGA GAGACATGTA CCGTATCCTT TTGCAGACAG CCGGAGTCGA GCTTCCACCA 
CAGGGTTCAG ATGGTGGATC TCAATCCACT ACTCCCAGCA GGCCAGGACC CATGGCCACT 
AGATCCACAC CACTAAGGGC TGCTGCTGCA GAATCTTTCC AGGCAACCCA GTCTAAAGCA 
GCACTTAAAC AGATAAATGA TGCCTTTACT ACATATAAAA AGGAGAAGGC CGAAAATGAC 
AAGCTTCTAA ATGAGCAGAT TGAGAGGCTT CGTGGTCAAG TGTCTGACCT GCTCTCACAA 
AAGGCCAAAC TGTCCTCTCA GCTGGAGTTT GCCTCTAAAC GGTATGATAT GCTTCAGGAG 
AATGTGAATG GCTTTCGAAG AGAGACTGAA GCCCTGCGGG AGAGGAATCA AAAGATGTCT 
GCCACCCATC AGAGACATGA GCAAATCATT CACACAATGA CTCAAGACCT GAGGGAGGCC 
AATGAGAAAC TGACCATGTC CGAGTCGCGG GCTGAAAACA TCCGTAAGGA ACGAGACATT 
CTGAAACAGG TGGAAAACAG ACTCAACCAG GAGAAAGAGT CTATCCTGAC TGAACAGCGG 
AGCCAGAACC TTCTGCTCAC CAACCTCAAA TCCATTCAGC TCACCATGGA GCGCTCTGAA 
ACTGAGACCA AACAGCGCTA CAATAATCAA ATCCAGCATC TGGAAAAGGA GATTATCCAG 
TTAAAGAAGA GGCTGGAGCA AGAAGTGGAA CAGAGACACG CACTGGAACG CAACCAAGAT 
ACTCAGCTGT TGGAGGCTAA GAAGAAGCTG GAGTCACAGA CCGCACTACA CCAGAAGACT 
AAAGAGCTGC TGAGATCTGC TGAACAGCAG GTGACCTCTT TGAGACAGCA ACTGAACAAC 
AATGAGAATC AGAGTGCAGC ACCTAAGCAG CCTGTCAGGA CATTACCCAG AGTTTCAGCT 
CCCAGCGTTC CTCAACAGGA GGCAGATGAT CTTCGTGCAC GTCTACAGCA GGTCGAGGAA 
CAGAACTCTG ACCTGAAAGA ACGCTTGAAG AACACCACCA CCAATGTAGA GCAGTATAGA 
TCTATGGTGC TCAGCTTGGA GGAGTCTGTG AACAAGGAGA AACAGTTGGC TGAGCAAGCA 
CGGACCTCCA CTGACAACCA ACTGAAGATG GCTCGGGAGT TGAATCAGCA GCTGGAAGCC 
CGTTTGGTGG AAGCGGAGAA GGAGAAGTTG GAGCTCCAGC AGGAGAAGAT GAAAGCTGTG 
GATAGTGTTG AAGAACGGGT GAAGGAGCTG AAAAGGAGCA TGACAAATAT GCAGACTGAG 
CTTCAGGAAG CTCTGCAGAG ATCTACAGCA GCAGTCACTC AGGAGCAGAA AGCCACACAG 
GACAGCAAAG TACAGGCTAA GCTGGCTGCA GAGGCTCAGA ATAAATACGA GCGAGAGCTC 
ATGCTGCATG CTGCAGATGT GGAAGCCCTG CAGGCCGCCA AGAAACAGGG ACAGCTTTTA 
ACACAAAACA TGAAGCAGCT CGAGGAGAAA GCTCAGAAAG CTGCATCTGA ACTGCATCAG 
TACCGCACAG ACTGGGTAAT GCAGGAGAAG AGACTAAAGG AAACGCTTTC TAAAGAGCAG 
AAGAGAGCCG AGGAGCTGCA GAAGCAGAAC ACTTTGCTCC ATGAGCAGAT GGAGAACTTG 
AGCAGCAAGA TCACTGCCTC AACCCAGCAG GAGACTGGAG AGAGCAGTCT GAACATCTCT 
CTGAACGAGG AGGGCAAGTC TCATGAGCAG ATACTGGACA TCCTCAGGTT TGTCCGGCGG 
GAGAAGGAGA TTGCAGAGAC CCGACTGGAA GTGGCCGAGG TGGAGACCCT TCGCTACAAA 
CAGCGCATGG AGTATCTGGA GTGCGAGCTG AAGGAACTAC AGGACAGCCT GAATGTAGAG 
AGAGAGAAGT TGCAGGTGAC AACAAAGACC TTGGTACAGC AGGAAGAGAC GATGAAGAGG 
ATGGAGAGCA TGAGTGCTCT TACAGATACC AACAAAATGC TGAAAAATGA GAAAAATCGA 
CTGGAGCATG AGCTGCAGCA GACTCAGGCC AAGATGCGTA AGCTCGAAGC AGACATCAAG 
CCTCTGCAGG AGGCCAATGG TGAGCTGAGT GAGAAGAGTG GCATGTTACA GGCTGAGAAG 
AGGCTTCTGG AGGAGGATGT CAAGCGCTTG AAGTCTCGCA CACAGCAACT CTTGAATCAG 
CAGAAGGACA GCGATCAGGA GGAAAGCAAG AAGCTTCATT CTGAGAGAGA GACTCATCTT 
AAACGCATCC AGCAGCTCAC AGAGGAGACT GGCAAACTGA AGAATGAGGT GGCCCGGAGC 
CAAGCTTCAA TCACCACGGC TCAGAGCCAA GTGCAGAACC TGCAAGACAG TCTGGGGAAG 
GTCACCACTG AACGAGACAG TCTGAAGAAG GAGCTGGAGG CAAAAACACT GGACATCCAG 
GAGAAAGTCA AAACCATCAC GCAGGTCAAG AAAATCGGCC GTCGCTACAA AACCCAGTAT 
GAAGAGCTCA AAGAACAGCA TGATAAGATG GTGGCAGAGG CTGCTTCTAA ACCAGCCCAG 
GAGCAGGAAG ACCGTCAGGC CTCAGTGCAG GAGATTCAGA ACCTGAAGAG CTCATTGAGT 
CAGACACAGA ATCGAACCTC AGAGCTGGAA ACTCAGCTGG ACTGCATACA GAAGACGTTG 
CAGGAGCGTG AAGCAGAGGT GAAGAGCCTA CAGGAACAGC TGACACAAGT GCAGCCTGAG 
CTCAGCCGTC TAAAAGCAGA ACTGCAGGAG AAGGGCAAAC TGGAGGAGCA GCTCCGACAG 
CAGATCGCTG ATAAAGAGGA GAAGACCAAG AAAGCCTTCT TGGGGGCCAA ACAGAAGCTC 
AACCAGCTCA ACAGTGCAAA GGAGCAGCTG GCGAAAGAAA ACGAGGAGCT GAAGCTGCAG 
CGAGAGGAGC TGGAGGTGAG GATGAGTGCA CTGAAGTCTC AATATGAAGG TCGACTTAGC 
CGACAAGAAC GAGAGCTGAG GGAACTTCGT GAACAGCAGG AGAGACATGG AGAACAGAGA 
GATGAAGCTC AGGAGCCTGG CCAGAGCAAG GTTCAAGAGG CACCGAGGTC ATCAGATGCT 
CGTCAGATCA CACTCAAGCC TACGCCTGCT GCTGACAGAG GAAGTGCAAG TCAGTCTGAA 
CCACCCACTG CCAACATCAA GCCCACGTTA GTTGCCGCCA CCCCTAGCAA GCCTTCACCA 
ATCGCAGGCA GCAAGTCCAC TCCTAAAGCA AGCATCCGTC CAATGATAAC GCCTGCTCCT 
GTTACCACAC CCACAGCCAC AGTCATGCCC ACCACACAGG TTGAAACGCA GGAAGCTCTA 
CAATCCACAG AGGGTCCGCT GGAGCATGTG ACTGTGTTTG GCAGCACCAG TGGCTCAGTG 
CGCTCCACCA GCCCAAACAT ACAGACCACA CAGCCAATCC TCAGCCTGCA GCAGAGCCAA 
GCCACTGCCT TCGTCCAGCC CACTCAGCAG CAGGCCAGCC AGGAACCAGC AACCAGCATT 
ATGGAAGCGG TGCATAGCTC TCAGATGGAG AGGCCCTCCA CCTCCACCGC TGTCTTTGGT 
ACAGTTTCTG CAACAACGGG TTCTTCGGTG GCCAAGCGAG TGCGAGAGGA GCATGAGGAG 
AGCTCCACTG AGGTGACGGA CACCACACAG GATGACTCTA CACATCCACC CATCATGAAG 
AAGCTCCGCA TTGTAAGAAG AGTGGGTCTG GAGGATGCTT CAGGTACTGA GGAGAGTAAT 
GATGCTATGG GTGAATCTTC AGGAGACGTT CAGCAGCCTC CTGATGCCAG TGAGGAAGCC 
TATCCCGTAT TAGGAGAGGG GGATGAAGAA TCATTGTCTG CCCCCATGGA TCAGGTGAGT 
GAGTCTCAAC CCTCTGAGTC TCAGATCAGT GGACAGGACT CATCAGAAGA GTACAAGCAT 
GATGTAATCG TCATCGAAAC AGACAGCGCC ACTGAGGAGG AGGAGGAAGA GGAAGAGGAG 
CAGGAAGAAC CTGGACAGTA TGAAGATGAT GATGAGGATG AGGACGATGA CGCAGAAGAT 
GAGGATGATG AAGATGGGGA TGGAGGAATG GCTGAAGCAG CAGAGGAGAG TAATGAAGGC 
AGTGGGAGTG CAGATGCCAA TGAGGCTTAT GAGGGGGATG AGGCAGAGGG TGCCGGGGAA 
GCTGATCCAA GTGTTCACAA TGAAGAGAGT CAAGAGGCAT CTGATTCAAC CCAGAAGCAA 
CCAGACTCTC AGAGCAGTGA AGCTAGTGTC AGCACCCTGG AGGCCTTCCC TCTTGAGCCT 
GTAAGAGAGC ACCCGCCGCT GCCCAGCACT ACCACCACCT CCCTCGCCCC GCGTCTGCCC 
CAATCCCCCC GTAGACCACA TCACACCCCT CCACCTCGTC TTAATATCCA CCCTGTCCCT 
GAACTTGGAC CTCCTATCAT GCAGAGGCAG TCTGGACAGA GCAGACGACC ATCAATGAGT 
CGCGCACCGC AGCTTACGCC CGGTATTGGA AGCATGCAAC ACTTCTTTGA TGACGATGAC 
AGAATGGTTC CCAGCACTCC CACTCTTGTG GTGCCACACC GCACAGATGG CTTTGCGGAG 
GCCATTCATT CCCCTCAGGT GGCTGGAGTT CCTCGCTTCC GTTTCGGCCC CCCAGAGGAC 
ATGATGCCTC AGGCCAGCTC TTCTCATTCT GACCTGAGCC ATCTGGCCAC ACATGGAGGA 
CTGGGCATGT ATGAAAGTCC ACTGTTCCTG CCCACTCATG ATGAGGAGTC TGGAGGCAGG 
AGTGTGCCCA CCACCCCTCT ACAAGTGGCC GCTCCAGTGA CCGTGTTCAC CGAAAGTCAC 
CCGTCGGATG TTTCGGAGAC GGCCTCTCAG TCAGTGCCCA TGGTAAGCAC ATCCACCGCC 
AGCCTGAGTG CACCAGGAGA GGCTGTGCCA GGAGATGATG GAGATGACGT GTTCGTGGCT 
GAGGCTGAGA GCGAAGGGGT GAGTAGTGAA GCGTGTCTAG AAAGCCAGTT GGAGCTAGAT 
TCTGCACAAC CGGCTGATGA TGCCAGTCTT CCCTCCACCA GTCAAGAGCC TACGTCTAGT 
TCTGCAGACA CTAGCACCAG TAGCGGTCAG GCTAAACCCG CAGGCCGTCT GCTTCCTCAG 
CACCATCTGC CCCGGAGAAA CCAGCTGATC CGGAGAGGTT TTCCTCGTGG AGGTCGGGGT 
CGAGGGCTCA GCAGAGGCTC CCCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296773.1
CDS	57..7106
Translation	MAALLQQALERTEVNKLPKAIQNKLEKVLAEQQTEIDSLISQHERYKADCEQQYFNLEKKLAESQEQFLSQSKDYHNIKEENNRLVEELKKLKEIEEVQGTTQESKPPRAKYEIEAENRELSRLLEKRSQEVENLSEDLKRLNDKLVETNTIKMELQLKLDELQSSEVSIQYREKRMEQEKELLQNQNTWLNTELKSKTDELYTVSRDKGKENLELRCSLESKKEEVTRLQDQVNTLKKANDSMQKSTEDLMRKLKEAKEQRASMEEKYRNELNANLKLCNLYKGAASDSEAKNTELNRAVEELNKLLKEAMEANKGTEKKLSELQSVREKAESDLREKVRHLEKELENSNTRLADFKRRGVPALTEEELTNLSPTAAAVAKIVKPGMKLMELYNAFVEAQDQLHLEKLENKRVNKVLDEIVLEVETKAPILKRQREEYESMQKSMTSLCYKLEQAMKEVHRLQKETDEANKRALVLERDNQKSERQLADMSEQVRVLLVEVEEARGNQVVREDVSSAVSSSSEVQGSRQVAFRSIQELQQQNQNLLVKIRDLEEQREKDQNQAKSAKQTELEQSLEKVQKELEQIKEQQNHQKLLAESAIRQRDMYRILLQTAGVELPPQGSDGGSQSTTPSRPGPMATRSTPLRAAAAESFQATQSKAALKQINDAFTTYKKEKAENDKLLNEQIERLRGQVSDLLSQKAKLSSQLEFASKRYDMLQENVNGFRRETEALRERNQKMSATHQRHEQIIHTMTQDLREANEKLTMSESRAENIRKERDILKQVENRLNQEKESILTEQRSQNLLLTNLKSIQLTMERSETETKQRYNNQIQHLEKEIIQLKKRLEQEVEQRHALERNQDTQLLEAKKKLESQTALHQKTKELLRSAEQQVTSLRQQLNNNENQSAAPKQPVRTLPRVSAPSVPQQEADDLRARLQQVEEQNSDLKERLKNTTTNVEQYRSMVLSLEESVNKEKQLAEQARTSTDNQLKMARELNQQLEARLVEAEKEKLELQQEKMKAVDSVEERVKELKRSMTNMQTELQEALQRSTAAVTQEQKATQDSKVQAKLAAEAQNKYERELMLHAADVEALQAAKKQGQLLTQNMKQLEEKAQKAASELHQYRTDWVMQEKRLKETLSKEQKRAEELQKQNTLLHEQMENLSSKITASTQQETGESSLNISLNEEGKSHEQILDILRFVRREKEIAETRLEVAEVETLRYKQRMEYLECELKELQDSLNVEREKLQVTTKTLVQQEETMKRMESMSALTDTNKMLKNEKNRLEHELQQTQAKMRKLEADIKPLQEANGELSEKSGMLQAEKRLLEEDVKRLKSRTQQLLNQQKDSDQEESKKLHSERETHLKRIQQLTEETGKLKNEVARSQASITTAQSQVQNLQDSLGKVTTERDSLKKELEAKTLDIQEKVKTITQVKKIGRRYKTQYEELKEQHDKMVAEAASKPAQEQEDRQASVQEIQNLKSSLSQTQNRTSELETQLDCIQKTLQEREAEVKSLQEQLTQVQPELSRLKAELQEKGKLEEQLRQQIADKEEKTKKAFLGAKQKLNQLNSAKEQLAKENEELKLQREELEVRMSALKSQYEGRLSRQERELRELREQQERHGEQRDEAQEPGQSKVQEAPRSSDARQITLKPTPAADRGSASQSEPPTANIKPTLVAATPSKPSPIAGSKSTPKASIRPMITPAPVTTPTATVMPTTQVETQEALQSTEGPLEHVTVFGSTSGSVRSTSPNIQTTQPILSLQQSQATAFVQPTQQQASQEPATSIMEAVHSSQMERPSTSTAVFGTVSATTGSSVAKRVREEHEESSTEVTDTTQDDSTHPPIMKKLRIVRRVGLEDASGTEESNDAMGESSGDVQQPPDASEEAYPVLGEGDEESLSAPMDQVSESQPSESQISGQDSSEEYKHDVIVIETDSATEEEEEEEEEQEEPGQYEDDDEDEDDDAEDEDDEDGDGGMAEAAEESNEGSGSADANEAYEGDEAEGAGEADPSVHNEESQEASDSTQKQPDSQSSEASVSTLEAFPLEPVREHPPLPSTTTTSLAPRLPQSPRRPHHTPPPRLNIHPVPELGPPIMQRQSGQSRRPSMSRAPQLTPGIGSMQHFFDDDDRMVPSTPTLVVPHRTDGFAEAIHSPQVAGVPRFRFGPPEDMMPQASSSHSDLSHLATHGGLGMYESPLFLPTHDEESGGRSVPTTPLQVAAPVTVFTESHPSDVSETASQSVPMVSTSTASLSAPGEAVPGDDGDDVFVAEAESEGVSSEACLESQLELDSAQPADDASLPSTSQEPTSSSADTSTSSGQAKPAGRLLPQHHLPRRNQLIRRGFPRGGRGRGLSRGSPF

Target ORF information:

RefSeq Version	XM_009298498.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio translocated promoter region a, nuclear basket protein (tpra), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298498.2

ORF Insert Sequence:

ATGGCGGCAC TTCTTCAGCA AGCGCTGGAA AGAACCGAGG TCAACAAACT GCCGAAAGCC 
ATTCAGAACA AGCTCGAGAA GGTTCTGGCA GAGCAGCAGA CGGAAATCGA CTCGTTGATA 
TCACAGCATG AGCGGTATAA AGCAGACTGT GAGCAACAGT ACTTTAATCT CGAGAAGAAG 
CTTGCTGAGA GTCAGGAGCA GTTTCTATCC CAAAGTAAGG ACTACCACAA TATTAAGGAG 
GAAAACAACC GTCTTGTGGA AGAGCTTAAG AAGTTAAAAG AGATAGAAGA AGTGCAGGGA 
ACAACGCAAG AGAGTAAGCC TCCTAGAGCC AAATATGAAA TCGAGGCAGA AAACCGAGAG 
CTGTCACGAT TGCTTGAGAA GAGATCTCAA GAAGTGGAAA ACCTCAGTGA GGATCTCAAA 
CGTCTCAATG ACAAACTTGT GGAGACCAAC ACAATCAAGA TGGAGCTCCA GCTTAAACTA 
GATGAACTTC AGTCTTCTGA GGTTTCCATT CAGTATCGTG AGAAGCGGAT GGAACAAGAA 
AAAGAGCTTC TCCAGAACCA GAACACATGG CTGAACACAG AGCTGAAGAG TAAGACGGAT 
GAGCTGTACA CCGTATCCAG AGACAAAGGC AAAGAGAACC TGGAGCTTAG GTGTTCTTTA 
GAGAGCAAGA AGGAAGAGGT GACCAGACTT CAGGATCAAG TAAACACACT CAAGAAAGCA 
AATGACAGCA TGCAGAAGAG TACAGAGGAT CTGATGAGGA AGCTAAAAGA GGCTAAAGAA 
CAACGGGCCT CAATGGAAGA GAAGTACCGC AATGAGCTGA ATGCAAACCT CAAACTATGT 
AATTTGTACA AGGGTGCAGC ATCAGATTCT GAAGCCAAGA ACACTGAACT GAACAGAGCT 
GTGGAAGAGC TCAATAAACT GCTGAAGGAA GCAATGGAAG CCAATAAAGG AACCGAGAAG 
AAGCTGTCAG AGTTGCAGAG CGTAAGGGAG AAAGCAGAGT CAGATTTACG TGAGAAGGTT 
AGGCATCTGG AGAAGGAGCT GGAGAACTCC AACACAAGGC TCGCAGACTT TAAGAGGAGA 
GGTGTTCCAG CTCTCACAGA AGAGGAGCTG ACAAACCTTT CTCCAACTGC AGCGGCAGTG 
GCCAAGATCG TGAAGCCGGG CATGAAGCTA ATGGAGTTGT ACAATGCCTT TGTGGAGGCA 
CAAGACCAGC TGCACCTTGA GAAGTTGGAG AACAAGCGTG TGAACAAGGT CCTTGATGAG 
ATCGTGCTGG AGGTGGAAAC CAAAGCTCCC ATCCTGAAAC GCCAGAGAGA GGAATATGAG 
AGCATGCAGA AGTCTATGAC CAGTCTCTGT TATAAACTAG AGCAAGCTAT GAAGGAGGTC 
CATCGGCTAC AAAAAGAAAC GGATGAAGCC AATAAGAGAG CTTTAGTGCT TGAAAGAGAT 
AATCAGAAAT CAGAGAGACA GCTAGCCGAC ATGTCTGAGC AGGTGCGTGT GCTTCTGGTG 
GAGGTGGAGG AGGCACGTGG TAATCAGGTA GTGCGTGAGG ATGTGAGTTC TGCTGTGAGC 
AGTAGCTCTG AGGTCCAGGG TTCACGACAG GTGGCATTCC GCAGCATCCA AGAGCTTCAG 
CAGCAGAACC AGAACTTACT GGTCAAGATC AGAGACCTGG AAGAGCAGAG AGAAAAAGAC 
CAGAACCAGG CTAAGAGTGC AAAGCAAACA GAATTGGAAC AAAGTCTGGA GAAGGTTCAG 
AAGGAGCTGG AGCAGATCAA GGAACAGCAA AACCATCAGA AGCTGCTGGC TGAATCTGCC 
ATCCGCCAGA GAGACATGTA CCGTATCCTT TTGCAGACAG CCGGAGTCGA GCTTCCACCA 
CAGGGTTCAG ATGGTGGATC TCAATCCACT ACTCCCAGCA GGCCAGGACC CATGGCCACT 
AGATCCACAC CACTAAGGGC TGCTGCTGCA GAATCTTTCC AGGCAACCCA GTCTAAAGCA 
GCACTTAAAC AGATAAATGA TGCCTTTACT ACATATAAAA AGGAGAAGGC CGAAAATGAC 
AAGCTTCTAA ATGAGCAGAT TGAGAGGCTT CGTGGTCAAG TGTCTGACCT GCTCTCACAA 
AAGGCCAAAC TGTCCTCTCA GCTGGAGTTT GCCTCTAAAC GGTATGATAT GCTTCAGGAG 
AATGTGAATG GCTTTCGAAG AGAGACTGAA GCCCTGCGGG AGAGGAATCA AAAGATGTCT 
GCCACCCATC AGAGACATGA GCAAATCATT CACACAATGA CTCAAGACCT GAGGGAGGCC 
AATGAGAAAC TGACCATGTC CGAGTCGCGG GCTGAAAACA TCCGTAAGGA ACGAGACATT 
CTGAAACAGG TGGAAAACAG ACTCAACCAG GAGAAAGAGT CTATCCTGAC TGAACAGCGG 
AGCCAGAACC TTCTGCTCAC CAACCTCAAA TCCATTCAGC TCACCATGGA GCGCTCTGAA 
ACTGAGACCA AACAGCGCTA CAATAATCAA ATCCAGCATC TGGAAAAGGA GATTATCCAG 
TTAAAGAAGA GGCTGGAGCA AGAAGTGGAA CAGAGACACG CACTGGAACG CAACCAAGAT 
ACTCAGCTGT TGGAGGCTAA GAAGAAGCTG GAGTCACAGA CCGCACTACA CCAGAAGACT 
AAAGAGCTGC TGAGATCTGC TGAACAGCAG GTGACCTCTT TGAGACAGCA ACTGAACAAC 
AATGAGAATC AGAGTGCAGC ACCTAAGCAG CCTGTCAGGA CATTACCCAG AGTTTCAGCT 
CCCAGCGTTC CTCAACAGGA GGCAGATGAT CTTCGTGCAC GTCTACAGCA GGTCGAGGAA 
CAGAACTCTG ACCTGAAAGA ACGCTTGAAG AACACCACCA CCAATGTAGA GCAGTATAGA 
TCTATGGTGC TCAGCTTGGA GGAGTCTGTG AACAAGGAGA AACAGTTGGC TGAGCAAGCA 
CGGACCTCCA CTGACAACCA ACTGAAGATG GCTCGGGAGT TGAATCAGCA GCTGGAAGCC 
CGTTTGGTGG AAGCGGAGAA GGAGAAGTTG GAGCTCCAGC AGGAGAAGAT GAAAGCTGTG 
GATAGTGTTG AAGAACGGGT GAAGGAGCTG AAAAGGAGCA TGACAAATAT GCAGACTGAG 
CTTCAGGAAG CTCTGCAGAG ATCTACAGCA GCAGTCACTC AGGAGCAGAA AGCCACACAG 
GACAGCAAAG TACAGGCTAA GCTGGCTGCA GAGGCTCAGA ATAAATACGA GCGAGAGCTC 
ATGCTGCATG CTGCAGATGT GGAAGCCCTG CAGGCCGCCA AGAAACAGGG ACAGCTTTTA 
ACACAAAACA TGAAGCAGCT CGAGGAGAAA GCTCAGAAAG CTGCATCTGA ACTGCATCAG 
TACCGCACAG ACTGGGTAAT GCAGGAGAAG AGACTAAAGG AAACGCTTTC TAAAGAGCAG 
AAGAGAGCCG AGGAGCTGCA GAAGCAGAAC ACTTTGCTCC ATGAGCAGAT GGAGAACTTG 
AGCAGCAAGA TCACTGCCTC AACCCAGCAG GAGACTGGAG AGAGCAGTCT GAACATCTCT 
CTGAACGAGG AGGGCAAGTC TCATGAGCAG ATACTGGACA TCCTCAGGTT TGTCCGGCGG 
GAGAAGGAGA TTGCAGAGAC CCGACTGGAA GTGGCCGAGG TGGAGACCCT TCGCTACAAA 
CAGCGCATGG AGTATCTGGA GTGCGAGCTG AAGGAACTAC AGGACAGCCT GAATGTAGAG 
AGAGAGAAGT TGCAGGTGAC AACAAAGACC TTGGTACAGC AGGAAGAGAC GATGAAGAGG 
ATGGAGAGCA TGAGTGCTCT TACAGATACC AACAAAATGC TGAAAAATGA GAAAAATCGA 
CTGGAGCATG AGCTGCAGCA GACTCAGGCC AAGATGCGTA AGCTCGAAGC AGACATCAAG 
CCTCTGCAGG AGGCCAATGG TGAGCTGAGT GAGAAGAGTG GCATGTTACA GGCTGAGAAG 
AGGCTTCTGG AGGAGGATGT CAAGCGCTTG AAGTCTCGCA CACAGCAACT CTTGAATCAG 
CAGAAGGACA GCGATCAGGA GGAAAGCAAG AAGCTTCATT CTGAGAGAGA GACTCATCTT 
AAACGCATCC AGCAGCTCAC AGAGGAGACT GGCAAACTGA AGAATGAGGT GGCCCGGAGC 
CAAGCTTCAA TCACCACGGC TCAGAGCCAA GTGCAGAACC TGCAAGACAG TCTGGGGAAG 
GTCACCACTG AACGAGACAG TCTGAAGAAG GAGCTGGAGG CAAAAACACT GGACATCCAG 
GAGAAAGTCA AAACCATCAC GCAGGTCAAG AAAATCGGCC GTCGCTACAA AACCCAGTAT 
GAAGAGCTCA AAGAACAGCA TGATAAGATG GTGGCAGAGG CTGCTTCTAA ACCAGCCCAG 
GAGCAGGAAG ACCGTCAGGC CTCAGTGCAG GAGATTCAGA ACCTGAAGAG CTCATTGAGT 
CAGACACAGA ATCGAACCTC AGAGCTGGAA ACTCAGCTGG ACTGCATACA GAAGACGTTG 
CAGGAGCGTG AAGCAGAGGT GAAGAGCCTA CAGGAACAGC TGACACAAGT GCAGCCTGAG 
CTCAGCCGTC TAAAAGCAGA ACTGCAGGAG AAGGGCAAAC TGGAGGAGCA GCTCCGACAG 
CAGATCGCTG ATAAAGAGGA GAAGACCAAG AAAGCCTTCT TGGGGGCCAA ACAGAAGCTC 
AACCAGCTCA ACAGTGCAAA GGAGCAGCTG GCGAAAGAAA ACGAGGAGCT GAAGCTGCAG 
CGAGAGGAGC TGGAGGTGAG GATGAGTGCA CTGAAGTCTC AATATGAAGG TCGACTTAGC 
CGACAAGAAC GAGAGCTGAG GGAACTTCGT GAACAGCAGG AGAGACATGG AGAACAGAGA 
GATGAAGCTC AGGAGCCTGG CCAGAGCAAG GTTCAAGAGG CACCGAGGTC ATCAGATGCT 
CGTCAGATCA CACTCAAGCC TACGCCTGCT GCTGACAGAG GAAGTGCAAG TCAGTCTGAA 
CCACCCACTG CCAACATCAA GCCCACGTTA GTTGCCGCCA CCCCTAGCAA GCCTTCACCA 
ATCGCAGGCA GCAAGTCCAC TCCTAAAGCA AGCATCCGTC CAATGATAAC GCCTGCTCCT 
GTTACCACAC CCACAGCCAC AGTCATGCCC ACCACACAGG TTGAAACGCA GGAAGCTCTA 
CAATCCACAG AGGGTCCGCT GGAGCATGTG ACTGTGTTTG GCAGCACCAG TGGCTCAGTG 
CGCTCCACCA GCCCAAACAT ACAGACCACA CAGCCAATCC TCAGCCTGCA GCAGAGCCAA 
GCCACTGCCT TCGTCCAGCC CACTCAGCAG CAGGCCAGCC AGGAACCAGC AACCAGCATT 
ATGGAAGCGG TGCATAGCTC TCAGATGGAG AGGCCCTCCA CCTCCACCGC TGTCTTTGGT 
ACAGTTTCTG CAACAACGGG TTCTTCGGTG GCCAAGCGAG TGCGAGAGGA GCATGAGGAG 
AGCTCCACTG AGGTGACGGA CACCACACAG GATGACTCTA CACATCCACC CATCATGAAG 
AAGCTCCGCA TTGTAAGAAG AGTGGGTCTG GAGGATGCTT CAGGTACTGA GGAGAGTAAT 
GATGCTATGG GTGAATCTTC AGGAGACGTT CAGCAGCCTC CTGATGCCAG TGAGGAAGCC 
TATCCCGTAT TAGGAGAGGG GGATGAAGAA TCATTGTCTG CCCCCATGGA TCAGGTGAGT 
GAGTCTCAAC CCTCTGAGTC TCAGATCAGT GGACAGGACT CATCAGAAGA GTACAAGCAT 
GATGTAATCG TCATCGAAAC AGACAGCGCC ACTGAGGAGG AGGAGGAAGA GGAAGAGGAG 
CAGGAAGAAC CTGGACAGTA TGAAGATGAT GATGAGGATG AGGACGATGA CGCAGAAGAT 
GAGGATGATG AAGATGGGGA TGGAGGAATG GCTGAAGCAG CAGAGGAGAG TAATGAAGGC 
AGTGGGAGTG CAGATGCCAA TGAGGCTTAT GAGGGGGATG AGGCAGAGGG TGCCGGGGAA 
GCTGATCCAA GTGTTCACAA TGAAGAGAGT CAAGAGGCAT CTGATTCAAC CCAGAAGCAA 
CCAGACTCTC AGAGCAGTGA AGCTAGTGTC AGCACCCTGG AGGCCTTCCC TCTTGAGCCT 
GTAAGAGAGC ACCCGCCGCT GCCCAGCACT ACCACCACCT CCCTCGCCCC GCGTCTGCCC 
CAATCCCCCC GTAGACCACA TCACACCCCT CCACCTCGTC TTAATATCCA CCCTGTCCCT 
GAACTTGGAC CTCCTATCAT GCAGAGGCAG TCTGGACAGA GCAGACGACC ATCAATGAGT 
CGCGCACCGC AGCTTACGCC CGGTATTGGA AGCATGCAAC ACTTCTTTGA TGACGATGAC 
AGAATGGTTC CCAGCACTCC CACTCTTGTG GTGCCACACC GCACAGATGG CTTTGCGGAG 
GCCATTCATT CCCCTCAGGT GGCTGGAGTT CCTCGCTTCC GTTTCGGCCC CCCAGAGGAC 
ATGATGCCTC AGGCCAGCTC TTCTCATTCT GACCTGAGCC ATCTGGCCAC ACATGGAGGA 
CTGGGCATGT ATGAAAGTCC ACTGTTCCTG CCCACTCATG ATGAGGAGTC TGGAGGCAGG 
AGTGTGCCCA CCACCCCTCT ACAAGTGGCC GCTCCAGTGA CCGTGTTCAC CGAAAGTCAC 
CCGTCGGATG TTTCGGAGAC GGCCTCTCAG TCAGTGCCCA TGGTAAGCAC ATCCACCGCC 
AGCCTGAGTG CACCAGGAGA GGCTGTGCCA GGAGATGATG GAGATGACGT GTTCGTGGCT 
GAGGCTGAGA GCGAAGGGGT GAGTAGTGAA GCGTGTCTAG AAAGCCAGTT GGAGCTAGAT 
TCTGCACAAC CGGCTGATGA TGCCAGTCTT CCCTCCACCA GTCAAGAGCC TACGTCTAGT 
TCTGCAGACA CTAGCACCAG TAGCGGTCAG GCTAAACCCG CAGGCCGTCT GCTTCCTCAG 
CACCATCTGC CCCGGAGAAA CCAGCTGATC CGGAGAGGTT TTCCTCGTGG AGGTCGGGGT 
CGAGGGCTCA GCAGAGGCTC CCCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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TPRA cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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