larsb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	larsb
Entrez Gene ID	569778
Full Name	leucyl-tRNA synthetase b
Synonyms	cb667,lars,wu:fb36b12
General protein information	Preferred Names leucyl-tRNA synthetase b Names leucyl-tRNA synthetase, cytoplasmic leucine--tRNA ligase, cytoplasmic
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_693187.6	XP_698279.6	leucine--tRNA ligase, cytoplasmic
XM_693187.7	XP_698279.6	leucine--tRNA ligase, cytoplasmic

Pathways from BioSystems

Homologies

Neurospora crassa	NCU09463	XP_963218.1
Homo sapiens (human)	LARS	NP_064502.9
Drosophila melanogaster (fruit fly)	CG33123	NP_787968.1
Oryza sativa (rice)	Os09g0378300	NP_001063048.1
Oryza sativa (rice)	Os09g0503400	NP_001063593.2
Rattus norvegicus (Norway rat)	Lars	NP_001009637.1
Danio rerio (zebrafish)	larsb	XP_698279.5
Caenorhabditis elegans (roundworm)	lars-1	NP_497837.1
Saccharomyces cerevisiae (baker's yeast)	CDC60	NP_015165.1
Arabidopsis thaliana (thale cress)	AT1G09620	NP_172433.2
Xenopus tropicalis (tropical clawed frog)	lars	NP_001015831.1
Kluyveromyces lactis	KLLA0D06105g	XP_453334.1
Canis lupus familiaris (dog)	LARS	XP_535229.3
Mus musculus (house mouse)	Lars	NP_598898.2
Gallus gallus (chicken)	LARS	XP_414663.2
Magnaporthe oryzae (rice blast fungus)	MGG_02432	XP_003709219.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008297	XP_317169.2
Pan troglodytes (chimpanzee)	LARS	XP_001157096.1
Bos taurus (cattle)	LARS	NP_001095962.1
Schizosaccharomyces pombe (fission yeast)	lrs1	NP_594882.1
Eremothecium gossypii	AGOS_AAL088W	NP_982454.2

Gene Ontology

The following larsb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the larsb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa56962	XM_693187.7 Latest version!	Danio rerio leucyl-tRNA synthetase b (larsb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODa56962	XM_693187.6	Danio rerio leucyl-tRNA synthetase b (larsb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

larsb ( XM_693187.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_698279.6 Danio rerio leucyl-tRNA synthetase b (larsb), mRNA.

Price & Availability↑

CloneID	ODa56962
Clone ID Related Accession (Same CDS sequence)	XM_693187.6 , XM_693187.7
Accession Version	XM_693187.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	leucine--tRNA ligase, cytoplasmic
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) and transcript sequence (GDQH01004359.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_693187.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1163 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGACGGAAC GCAAAGGAAC AGCAAAACTT GACTTCTTGA AGAAGATTGA GGAGGAGGTT 
CAGCAGAAAT GGGAGCAGGA CAAGCTTTTT GAGAATGAAG CCCCAGCCAC CATTGGGGAA 
AATACGAATA AGAACAAGTA CTTTATTACA TTTCCCTACC CATATATGAA TGGGAGACTT 
CACCTCGGCC ATACATTCTG CCTTTCCAAG TGTGAGTTTG CAGTGGGTTA CCAGCGGCTT 
AAAGGAAAGC AGTGTCTTTT CCCATTTGGG CTTCACTGCA CTGGGATGCC TATCAAAGCA 
TGTGCAGACA AGCTGAAGAG AGAAATGGAG CTGTATGGTT ATCCGCCCCA ATTCCCTGAA 
GAAGATGAAG AGGAGGAAGA GCAGAAGAAG ACCTCTGATG ACGTTATCAT CAAGGACAAG 
ACAAAGGGCA AGAAGAGCAA AGCTGTAGCA AAGTCTGGAA GCTCCAAGTT CCAGTGGGAC 
ATCATGAAAT CTCTGGGACT GCAGGATGCT GAAATTGTGA AGTTTGCTGA AGCTGAACAT 
TGGCTGGATT ATTTCCCTCC TCTTGCTGTA GAAGATCTGA AGAGAATGGG TCTAAAGGTG 
GATTGGCGGC GTTCCTTTAT TACCACTGAT GTAAACCCCT TTTACGACTC TTTTGTGAGG 
TGGCAGTTCA TCAATTTGAA AGAGAGAAAG AAGATCAAGT TTGGGAAAAG ATATACAATT 
TACTCTCCTA AAGATGGGCA GCCCTGCATG GATCATGACA GACAGACAGG AGAGGGAGTT 
GGTCCTCAAG AATATACTCT AATCAAGATG AAGGTTGTGG AACCTTACCC AACAAAGCTG 
GGTGCTCTTA AAGGCAAGAC CGTTTTTCTG GTTGCTGCAA CACTGAGGCC TGAAACCATG 
TTTGGTCAGA CGAATTGCTG GATTCGTCCA GACATGAAGT ACGTTGTGTT TGAGACCGCC 
AATGGTGACT TGTTCATCAG CACTCAGCGA TCAGCCAGGA ACATGTCCTA CCAAGGCTTT 
ACCAAAGAGA ATGGAGTGGT TCCTGTGATC ATGAACATCA TGGGACAGGA CATCCTTGGC 
TGTGCGTTGA GCGCTCCTCT CACCTCATAC AAGACCATTT ATGCCCTGCC AATGCTCACC 
ATTAAGGAAG ACAAAGGAAC TGGAGTCGTC ACCAGTGTGC CGTCAGATGC ACCGGATGAT 
ATTGCAGCTC TAAGGGACAT TAAAAAGAAA CAGGCATTGA GAGAGAAGTA TGGAATCCAA 
GATTTTATGG TGCTACCATT TGAGCCGGTT CCCATCATTG AAATTCCAGG GTATGGGAAT 
CTGTCAGCTC CACTGGTGTG CGATGAACTG AAGATCCAAA GCCAGAATGA CAGAGAGAAA 
CTGGCCGAGG CTAAGGAGAA GGTCTACCTC AAGGGTTTTT ACGAAGGGAT CATGTTGGTG 
GAAGGTTTCA AAGGACAGAA GGTCCAGGAT GTGAAGAAGC CCATTCAGAA AATGATGGTG 
GAAAAGGGTG AAGCACTGAT TTACATGGAG CCTGAGAAGC AGGTGTTATC TCGGTCTGCA 
GACGAGTGTG TTGTGGCTTT GTGTGACCAG TGGTATCTGG ATTATGGCAA TGAGGAATGG 
AAAAAGCAGG CTCACGGTGT CCTGGAGCAT CTGGAGACGT TCTGTGATGA AACCAGAAAG 
AACTTTGAAG CAACGTTAGC TTGGCTTCAG GAGCACGCCT GTTCCAGAAC GTATGGATTA 
GGAACCCGGT TGCCATGGAA TGAACAATGG CTCATTGAGT CCCTGTCCGA CTCCACCATC 
TATATGGCCT ACTACACCGT GGCACATCTC CTCCAGGGAG GGGTCCTCAG TGGACAAGGA 
CCTTCGCCAC TGGGAATCAG ACCCCAGCAG ATGACGAGGG AGGTGTGGGA TTACATCTTC 
TTCAAAACAT CACCTTTCCC AAAGACGGAC ATCCCGAAGG AGCATCTGCT GAAGCTCAGA 
CGGGAGTTTG AGTTCTGGTA CCCTGTAGAC GTCAGAGTAT CGGGAAAAGA TCTGGTGCCA 
AACCATCTCT CCTATTATCT CTACAATCAT GTAGCCATGT GGCCCAATGA CAGTGGAAAA 
TGGCCGCAAG CTGTACGTGC CAATGGTCAT CTTTTGCTAA ACTCTGAAAA GATGTCCAAA 
TCCACTGGCA ACTTTCTCAC TTTGAGTCAA GCCATTGCCA AGTTTTCAGC AGATGGTATG 
CGTTTGGCTT TGGCTGATGC GGGAGACACT GTGGAGGATG CTAACTTTGT TGAGGCTATG 
GCGGATGCGG GAATACTGCG TCTCTTTACT TGGGTGGAAT GGGTGAAGGA GATGATCGCT 
AATCAGGACA ACCTAAGGAC CGGACCTGCA GACACATTTA ATGACAGAGT GTTTATAAGT 
GAAATGAACT CCGGCATCAT TAAAACAGAT CAGCACTATG AGAGAATGAT GTACAAGGAG 
GCATTGAAGA GTGGCTTCTT TGAATTCCAG GCTGCCAAGG ATAAATACAG GGAGCTGGCC 
ATCGAGGGCA TGCACAAAGA TCTCGTGTTT CAGTTTATCG AGAACCAAAC ACTCCTGCTG 
GCTCCCATCT GCCCACATCT GTGTGAACAC ACCTGGTCCC TGTTGGGAAA GAGCAGTACT 
GTAATGAAGG CTCAATGGCC TACAGCTGGT CCAGTGGACG AGATCTTGAT TCGATCTTCA 
CAGTATCTGA TGGACACTGC CCATGACCTC CGCCTTCGCC TGAAAGCCTA CACACAGCCA 
GCCAAGGGCA AGAAGGGGGA CAACAAACCA CCAGCTAAGC CCACGCACTG CACCATCTAT 
GTTGCAAAGA CCTACCCACC CTGGCAGCAC AGCGCCCTCT CTCTGCTTGG CAAACATTAC 
AAGAGCAACA ATGGAGCTTT ACCTGACAAT AAGGTTATCG CCATGGAGCT CGGAGCCATG 
CCTGAGCTGA AGAAATACAT GAAGAGAGTC ATGCCATTTG TGGCTATGAT AAAGGACAAC 
CTAGAGAAAA ACGGGCCCAG GGTTCTTGAC CTAGAACTGG AGTTCGATGA GCGTGCTGTT 
CTGCTGGAAA ACATTGTTTA TCTGACTAAC TCACTTGAGT TGGATCAGAT AGACGTTGTG 
TTTGCCTCTG AAGCGGATGA GAAAGTAAAG GAGGACTGTT GCCCTGGGAA ACCGTTCTGT 
GTCTTCAGAT CTGAGCCTGG AGTGATAATC TCACTGGTGA ACCCTCAGCC ATCCAACGGT 
CTGTTCTCCA CCAAGATAGA CATCCGACAG GGAGACAGCA AAGACAGCAT CATTCGCAGA 
CTGTCCCGAG TCAACAGAGG CATCAAGGAT TTGTCCAAAG TGAAGCTGAT GCGTTATGAG 
GATCCTGTTC TCGGACCACG GAGAATCCCA GTTTTGGGGA AGGAAGAAGA AGGCAAACTT 
CCAGTCTCGA ACAGTTCAGT CTTCCACATC AGCTTGCAAG AAAGCAAAGT TCATATGAGC 
GACAACGGCC TGAAAGTGGA CATTGGAGAT ACGTTAATCT ACCTGGTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_698279.6
CDS	96..3626
Translation	MTERKGTAKLDFLKKIEEEVQQKWEQDKLFENEAPATIGENTNKNKYFITFPYPYMNGRLHLGHTFCLSKCEFAVGYQRLKGKQCLFPFGLHCTGMPIKACADKLKREMELYGYPPQFPEEDEEEEEQKKTSDDVIIKDKTKGKKSKAVAKSGSSKFQWDIMKSLGLQDAEIVKFAEAEHWLDYFPPLAVEDLKRMGLKVDWRRSFITTDVNPFYDSFVRWQFINLKERKKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLIKMKVVEPYPTKLGALKGKTVFLVAATLRPETMFGQTNCWIRPDMKYVVFETANGDLFISTQRSARNMSYQGFTKENGVVPVIMNIMGQDILGCALSAPLTSYKTIYALPMLTIKEDKGTGVVTSVPSDAPDDIAALRDIKKKQALREKYGIQDFMVLPFEPVPIIEIPGYGNLSAPLVCDELKIQSQNDREKLAEAKEKVYLKGFYEGIMLVEGFKGQKVQDVKKPIQKMMVEKGEALIYMEPEKQVLSRSADECVVALCDQWYLDYGNEEWKKQAHGVLEHLETFCDETRKNFEATLAWLQEHACSRTYGLGTRLPWNEQWLIESLSDSTIYMAYYTVAHLLQGGVLSGQGPSPLGIRPQQMTREVWDYIFFKTSPFPKTDIPKEHLLKLRREFEFWYPVDVRVSGKDLVPNHLSYYLYNHVAMWPNDSGKWPQAVRANGHLLLNSEKMSKSTGNFLTLSQAIAKFSADGMRLALADAGDTVEDANFVEAMADAGILRLFTWVEWVKEMIANQDNLRTGPADTFNDRVFISEMNSGIIKTDQHYERMMYKEALKSGFFEFQAAKDKYRELAIEGMHKDLVFQFIENQTLLLAPICPHLCEHTWSLLGKSSTVMKAQWPTAGPVDEILIRSSQYLMDTAHDLRLRLKAYTQPAKGKKGDNKPPAKPTHCTIYVAKTYPPWQHSALSLLGKHYKSNNGALPDNKVIAMELGAMPELKKYMKRVMPFVAMIKDNLEKNGPRVLDLELEFDERAVLLENIVYLTNSLELDQIDVVFASEADEKVKEDCCPGKPFCVFRSEPGVIISLVNPQPSNGLFSTKIDIRQGDSKDSIIRRLSRVNRGIKDLSKVKLMRYEDPVLGPRRIPVLGKEEEGKLPVSNSSVFHISLQESKVHMSDNGLKVDIGDTLIYLV

Target ORF information:

RefSeq Version	XM_693187.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio leucyl-tRNA synthetase b (larsb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_693187.6

ORF Insert Sequence:

ATGACGGAAC GCAAAGGAAC AGCAAAACTT GACTTCTTGA AGAAGATTGA GGAGGAGGTT 
CAGCAGAAAT GGGAGCAGGA CAAGCTTTTT GAGAATGAAG CCCCAGCCAC CATTGGGGAA 
AATACGAATA AGAACAAGTA CTTTATTACA TTTCCCTACC CATATATGAA TGGGAGACTT 
CACCTCGGCC ATACATTCTG CCTTTCCAAG TGTGAGTTTG CAGTGGGTTA CCAGCGGCTT 
AAAGGAAAGC AGTGTCTTTT CCCATTTGGG CTTCACTGCA CTGGGATGCC TATCAAAGCA 
TGTGCAGACA AGCTGAAGAG AGAAATGGAG CTGTATGGTT ATCCGCCCCA ATTCCCTGAA 
GAAGATGAAG AGGAGGAAGA GCAGAAGAAG ACCTCTGATG ACGTTATCAT CAAGGACAAG 
ACAAAGGGCA AGAAGAGCAA AGCTGTAGCA AAGTCTGGAA GCTCCAAGTT CCAGTGGGAC 
ATCATGAAAT CTCTGGGACT GCAGGATGCT GAAATTGTGA AGTTTGCTGA AGCTGAACAT 
TGGCTGGATT ATTTCCCTCC TCTTGCTGTA GAAGATCTGA AGAGAATGGG TCTAAAGGTG 
GATTGGCGGC GTTCCTTTAT TACCACTGAT GTAAACCCCT TTTACGACTC TTTTGTGAGG 
TGGCAGTTCA TCAATTTGAA AGAGAGAAAG AAGATCAAGT TTGGGAAAAG ATATACAATT 
TACTCTCCTA AAGATGGGCA GCCCTGCATG GATCATGACA GACAGACAGG AGAGGGAGTT 
GGTCCTCAAG AATATACTCT AATCAAGATG AAGGTTGTGG AACCTTACCC AACAAAGCTG 
GGTGCTCTTA AAGGCAAGAC CGTTTTTCTG GTTGCTGCAA CACTGAGGCC TGAAACCATG 
TTTGGTCAGA CGAATTGCTG GATTCGTCCA GACATGAAGT ACGTTGTGTT TGAGACCGCC 
AATGGTGACT TGTTCATCAG CACTCAGCGA TCAGCCAGGA ACATGTCCTA CCAAGGCTTT 
ACCAAAGAGA ATGGAGTGGT TCCTGTGATC ATGAACATCA TGGGACAGGA CATCCTTGGC 
TGTGCGTTGA GCGCTCCTCT CACCTCATAC AAGACCATTT ATGCCCTGCC AATGCTCACC 
ATTAAGGAAG ACAAAGGAAC TGGAGTCGTC ACCAGTGTGC CGTCAGATGC ACCGGATGAT 
ATTGCAGCTC TAAGGGACAT TAAAAAGAAA CAGGCATTGA GAGAGAAGTA TGGAATCCAA 
GATTTTATGG TGCTACCATT TGAGCCGGTT CCCATCATTG AAATTCCAGG GTATGGGAAT 
CTGTCAGCTC CACTGGTGTG CGATGAACTG AAGATCCAAA GCCAGAATGA CAGAGAGAAA 
CTGGCCGAGG CTAAGGAGAA GGTCTACCTC AAGGGTTTTT ACGAAGGGAT CATGTTGGTG 
GAAGGTTTCA AAGGACAGAA GGTCCAGGAT GTGAAGAAGC CCATTCAGAA AATGATGGTG 
GAAAAGGGTG AAGCACTGAT TTACATGGAG CCTGAGAAGC AGGTGTTATC TCGGTCTGCA 
GACGAGTGTG TTGTGGCTTT GTGTGACCAG TGGTATCTGG ATTATGGCAA TGAGGAATGG 
AAAAAGCAGG CTCACGGTGT CCTGGAGCAT CTGGAGACGT TCTGTGATGA AACCAGAAAG 
AACTTTGAAG CAACGTTAGC TTGGCTTCAG GAGCACGCCT GTTCCAGAAC GTATGGATTA 
GGAACCCGGT TGCCATGGAA TGAACAATGG CTCATTGAGT CCCTGTCCGA CTCCACCATC 
TATATGGCCT ACTACACCGT GGCACATCTC CTCCAGGGAG GGGTCCTCAG TGGACAAGGA 
CCTTCGCCAC TGGGAATCAG ACCCCAGCAG ATGACGAGGG AGGTGTGGGA TTACATCTTC 
TTCAAAACAT CACCTTTCCC AAAGACGGAC ATCCCGAAGG AGCATCTGCT GAAGCTCAGA 
CGGGAGTTTG AGTTCTGGTA CCCTGTAGAC GTCAGAGTAT CGGGAAAAGA TCTGGTGCCA 
AACCATCTCT CCTATTATCT CTACAATCAT GTAGCCATGT GGCCCAATGA CAGTGGAAAA 
TGGCCGCAAG CTGTACGTGC CAATGGTCAT CTTTTGCTAA ACTCTGAAAA GATGTCCAAA 
TCCACTGGCA ACTTTCTCAC TTTGAGTCAA GCCATTGCCA AGTTTTCAGC AGATGGTATG 
CGTTTGGCTT TGGCTGATGC GGGAGACACT GTGGAGGATG CTAACTTTGT TGAGGCTATG 
GCGGATGCGG GAATACTGCG TCTCTTTACT TGGGTGGAAT GGGTGAAGGA GATGATCGCT 
AATCAGGACA ACCTAAGGAC CGGACCTGCA GACACATTTA ATGACAGAGT GTTTATAAGT 
GAAATGAACT CCGGCATCAT TAAAACAGAT CAGCACTATG AGAGAATGAT GTACAAGGAG 
GCATTGAAGA GTGGCTTCTT TGAATTCCAG GCTGCCAAGG ATAAATACAG GGAGCTGGCC 
ATCGAGGGCA TGCACAAAGA TCTCGTGTTT CAGTTTATCG AGAACCAAAC ACTCCTGCTG 
GCTCCCATCT GCCCACATCT GTGTGAACAC ACCTGGTCCC TGTTGGGAAA GAGCAGTACT 
GTAATGAAGG CTCAATGGCC TACAGCTGGT CCAGTGGACG AGATCTTGAT TCGATCTTCA 
CAGTATCTGA TGGACACTGC CCATGACCTC CGCCTTCGCC TGAAAGCCTA CACACAGCCA 
GCCAAGGGCA AGAAGGGGGA CAACAAACCA CCAGCTAAGC CCACGCACTG CACCATCTAT 
GTTGCAAAGA CCTACCCACC CTGGCAGCAC AGCGCCCTCT CTCTGCTTGG CAAACATTAC 
AAGAGCAACA ATGGAGCTTT ACCTGACAAT AAGGTTATCG CCATGGAGCT CGGAGCCATG 
CCTGAGCTGA AGAAATACAT GAAGAGAGTC ATGCCATTTG TGGCTATGAT AAAGGACAAC 
CTAGAGAAAA ACGGGCCCAG GGTTCTTGAC CTAGAACTGG AGTTCGATGA GCGTGCTGTT 
CTGCTGGAAA ACATTGTTTA TCTGACTAAC TCACTTGAGT TGGATCAGAT AGACGTTGTG 
TTTGCCTCTG AAGCGGATGA GAAAGTAAAG GAGGACTGTT GCCCTGGGAA ACCGTTCTGT 
GTCTTCAGAT CTGAGCCTGG AGTGATAATC TCACTGGTGA ACCCTCAGCC ATCCAACGGT 
CTGTTCTCCA CCAAGATAGA CATCCGACAG GGAGACAGCA AAGACAGCAT CATTCGCAGA 
CTGTCCCGAG TCAACAGAGG CATCAAGGAT TTGTCCAAAG TGAAGCTGAT GCGTTATGAG 
GATCCTGTTC TCGGACCACG GAGAATCCCA GTTTTGGGGA AGGAAGAAGA AGGCAAACTT 
CCAGTCTCGA ACAGTTCAGT CTTCCACATC AGCTTGCAAG AAAGCAAAGT TCATATGAGC 
GACAACGGCC TGAAAGTGGA CATTGGAGAT ACGTTAATCT ACCTGGTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

larsb ( XM_693187.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_698279.6 Danio rerio leucyl-tRNA synthetase b (larsb), mRNA.

Price & Availability↑

CloneID	ODa56962
Clone ID Related Accession (Same CDS sequence)	XM_693187.6 , XM_693187.7
Accession Version	XM_693187.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	leucine--tRNA ligase, cytoplasmic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) and transcript sequence (GDQH01004359.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_693187.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1163 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGACGGAAC GCAAAGGAAC AGCAAAACTT GACTTCTTGA AGAAGATTGA GGAGGAGGTT 
CAGCAGAAAT GGGAGCAGGA CAAGCTTTTT GAGAATGAAG CCCCAGCCAC CATTGGGGAA 
AATACGAATA AGAACAAGTA CTTTATTACA TTTCCCTACC CATATATGAA TGGGAGACTT 
CACCTCGGCC ATACATTCTG CCTTTCCAAG TGTGAGTTTG CAGTGGGTTA CCAGCGGCTT 
AAAGGAAAGC AGTGTCTTTT CCCATTTGGG CTTCACTGCA CTGGGATGCC TATCAAAGCA 
TGTGCAGACA AGCTGAAGAG AGAAATGGAG CTGTATGGTT ATCCGCCCCA ATTCCCTGAA 
GAAGATGAAG AGGAGGAAGA GCAGAAGAAG ACCTCTGATG ACGTTATCAT CAAGGACAAG 
ACAAAGGGCA AGAAGAGCAA AGCTGTAGCA AAGTCTGGAA GCTCCAAGTT CCAGTGGGAC 
ATCATGAAAT CTCTGGGACT GCAGGATGCT GAAATTGTGA AGTTTGCTGA AGCTGAACAT 
TGGCTGGATT ATTTCCCTCC TCTTGCTGTA GAAGATCTGA AGAGAATGGG TCTAAAGGTG 
GATTGGCGGC GTTCCTTTAT TACCACTGAT GTAAACCCCT TTTACGACTC TTTTGTGAGG 
TGGCAGTTCA TCAATTTGAA AGAGAGAAAG AAGATCAAGT TTGGGAAAAG ATATACAATT 
TACTCTCCTA AAGATGGGCA GCCCTGCATG GATCATGACA GACAGACAGG AGAGGGAGTT 
GGTCCTCAAG AATATACTCT AATCAAGATG AAGGTTGTGG AACCTTACCC AACAAAGCTG 
GGTGCTCTTA AAGGCAAGAC CGTTTTTCTG GTTGCTGCAA CACTGAGGCC TGAAACCATG 
TTTGGTCAGA CGAATTGCTG GATTCGTCCA GACATGAAGT ACGTTGTGTT TGAGACCGCC 
AATGGTGACT TGTTCATCAG CACTCAGCGA TCAGCCAGGA ACATGTCCTA CCAAGGCTTT 
ACCAAAGAGA ATGGAGTGGT TCCTGTGATC ATGAACATCA TGGGACAGGA CATCCTTGGC 
TGTGCGTTGA GCGCTCCTCT CACCTCATAC AAGACCATTT ATGCCCTGCC AATGCTCACC 
ATTAAGGAAG ACAAAGGAAC TGGAGTCGTC ACCAGTGTGC CGTCAGATGC ACCGGATGAT 
ATTGCAGCTC TAAGGGACAT TAAAAAGAAA CAGGCATTGA GAGAGAAGTA TGGAATCCAA 
GATTTTATGG TGCTACCATT TGAGCCGGTT CCCATCATTG AAATTCCAGG GTATGGGAAT 
CTGTCAGCTC CACTGGTGTG CGATGAACTG AAGATCCAAA GCCAGAATGA CAGAGAGAAA 
CTGGCCGAGG CTAAGGAGAA GGTCTACCTC AAGGGTTTTT ACGAAGGGAT CATGTTGGTG 
GAAGGTTTCA AAGGACAGAA GGTCCAGGAT GTGAAGAAGC CCATTCAGAA AATGATGGTG 
GAAAAGGGTG AAGCACTGAT TTACATGGAG CCTGAGAAGC AGGTGTTATC TCGGTCTGCA 
GACGAGTGTG TTGTGGCTTT GTGTGACCAG TGGTATCTGG ATTATGGCAA TGAGGAATGG 
AAAAAGCAGG CTCACGGTGT CCTGGAGCAT CTGGAGACGT TCTGTGATGA AACCAGAAAG 
AACTTTGAAG CAACGTTAGC TTGGCTTCAG GAGCACGCCT GTTCCAGAAC GTATGGATTA 
GGAACCCGGT TGCCATGGAA TGAACAATGG CTCATTGAGT CCCTGTCCGA CTCCACCATC 
TATATGGCCT ACTACACCGT GGCACATCTC CTCCAGGGAG GGGTCCTCAG TGGACAAGGA 
CCTTCGCCAC TGGGAATCAG ACCCCAGCAG ATGACGAGGG AGGTGTGGGA TTACATCTTC 
TTCAAAACAT CACCTTTCCC AAAGACGGAC ATCCCGAAGG AGCATCTGCT GAAGCTCAGA 
CGGGAGTTTG AGTTCTGGTA CCCTGTAGAC GTCAGAGTAT CGGGAAAAGA TCTGGTGCCA 
AACCATCTCT CCTATTATCT CTACAATCAT GTAGCCATGT GGCCCAATGA CAGTGGAAAA 
TGGCCGCAAG CTGTACGTGC CAATGGTCAT CTTTTGCTAA ACTCTGAAAA GATGTCCAAA 
TCCACTGGCA ACTTTCTCAC TTTGAGTCAA GCCATTGCCA AGTTTTCAGC AGATGGTATG 
CGTTTGGCTT TGGCTGATGC GGGAGACACT GTGGAGGATG CTAACTTTGT TGAGGCTATG 
GCGGATGCGG GAATACTGCG TCTCTTTACT TGGGTGGAAT GGGTGAAGGA GATGATCGCT 
AATCAGGACA ACCTAAGGAC CGGACCTGCA GACACATTTA ATGACAGAGT GTTTATAAGT 
GAAATGAACT CCGGCATCAT TAAAACAGAT CAGCACTATG AGAGAATGAT GTACAAGGAG 
GCATTGAAGA GTGGCTTCTT TGAATTCCAG GCTGCCAAGG ATAAATACAG GGAGCTGGCC 
ATCGAGGGCA TGCACAAAGA TCTCGTGTTT CAGTTTATCG AGAACCAAAC ACTCCTGCTG 
GCTCCCATCT GCCCACATCT GTGTGAACAC ACCTGGTCCC TGTTGGGAAA GAGCAGTACT 
GTAATGAAGG CTCAATGGCC TACAGCTGGT CCAGTGGACG AGATCTTGAT TCGATCTTCA 
CAGTATCTGA TGGACACTGC CCATGACCTC CGCCTTCGCC TGAAAGCCTA CACACAGCCA 
GCCAAGGGCA AGAAGGGGGA CAACAAACCA CCAGCTAAGC CCACGCACTG CACCATCTAT 
GTTGCAAAGA CCTACCCACC CTGGCAGCAC AGCGCCCTCT CTCTGCTTGG CAAACATTAC 
AAGAGCAACA ATGGAGCTTT ACCTGACAAT AAGGTTATCG CCATGGAGCT CGGAGCCATG 
CCTGAGCTGA AGAAATACAT GAAGAGAGTC ATGCCATTTG TGGCTATGAT AAAGGACAAC 
CTAGAGAAAA ACGGGCCCAG GGTTCTTGAC CTAGAACTGG AGTTCGATGA GCGTGCTGTT 
CTGCTGGAAA ACATTGTTTA TCTGACTAAC TCACTTGAGT TGGATCAGAT AGACGTTGTG 
TTTGCCTCTG AAGCGGATGA GAAAGTAAAG GAGGACTGTT GCCCTGGGAA ACCGTTCTGT 
GTCTTCAGAT CTGAGCCTGG AGTGATAATC TCACTGGTGA ACCCTCAGCC ATCCAACGGT 
CTGTTCTCCA CCAAGATAGA CATCCGACAG GGAGACAGCA AAGACAGCAT CATTCGCAGA 
CTGTCCCGAG TCAACAGAGG CATCAAGGAT TTGTCCAAAG TGAAGCTGAT GCGTTATGAG 
GATCCTGTTC TCGGACCACG GAGAATCCCA GTTTTGGGGA AGGAAGAAGA AGGCAAACTT 
CCAGTCTCGA ACAGTTCAGT CTTCCACATC AGCTTGCAAG AAAGCAAAGT TCATATGAGC 
GACAACGGCC TGAAAGTGGA CATTGGAGAT ACGTTAATCT ACCTGGTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_698279.6
CDS	96..3626
Translation	MTERKGTAKLDFLKKIEEEVQQKWEQDKLFENEAPATIGENTNKNKYFITFPYPYMNGRLHLGHTFCLSKCEFAVGYQRLKGKQCLFPFGLHCTGMPIKACADKLKREMELYGYPPQFPEEDEEEEEQKKTSDDVIIKDKTKGKKSKAVAKSGSSKFQWDIMKSLGLQDAEIVKFAEAEHWLDYFPPLAVEDLKRMGLKVDWRRSFITTDVNPFYDSFVRWQFINLKERKKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLIKMKVVEPYPTKLGALKGKTVFLVAATLRPETMFGQTNCWIRPDMKYVVFETANGDLFISTQRSARNMSYQGFTKENGVVPVIMNIMGQDILGCALSAPLTSYKTIYALPMLTIKEDKGTGVVTSVPSDAPDDIAALRDIKKKQALREKYGIQDFMVLPFEPVPIIEIPGYGNLSAPLVCDELKIQSQNDREKLAEAKEKVYLKGFYEGIMLVEGFKGQKVQDVKKPIQKMMVEKGEALIYMEPEKQVLSRSADECVVALCDQWYLDYGNEEWKKQAHGVLEHLETFCDETRKNFEATLAWLQEHACSRTYGLGTRLPWNEQWLIESLSDSTIYMAYYTVAHLLQGGVLSGQGPSPLGIRPQQMTREVWDYIFFKTSPFPKTDIPKEHLLKLRREFEFWYPVDVRVSGKDLVPNHLSYYLYNHVAMWPNDSGKWPQAVRANGHLLLNSEKMSKSTGNFLTLSQAIAKFSADGMRLALADAGDTVEDANFVEAMADAGILRLFTWVEWVKEMIANQDNLRTGPADTFNDRVFISEMNSGIIKTDQHYERMMYKEALKSGFFEFQAAKDKYRELAIEGMHKDLVFQFIENQTLLLAPICPHLCEHTWSLLGKSSTVMKAQWPTAGPVDEILIRSSQYLMDTAHDLRLRLKAYTQPAKGKKGDNKPPAKPTHCTIYVAKTYPPWQHSALSLLGKHYKSNNGALPDNKVIAMELGAMPELKKYMKRVMPFVAMIKDNLEKNGPRVLDLELEFDERAVLLENIVYLTNSLELDQIDVVFASEADEKVKEDCCPGKPFCVFRSEPGVIISLVNPQPSNGLFSTKIDIRQGDSKDSIIRRLSRVNRGIKDLSKVKLMRYEDPVLGPRRIPVLGKEEEGKLPVSNSSVFHISLQESKVHMSDNGLKVDIGDTLIYLV

Target ORF information:

RefSeq Version	XM_693187.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leucyl-tRNA synthetase b (larsb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_693187.7

ORF Insert Sequence:

ATGACGGAAC GCAAAGGAAC AGCAAAACTT GACTTCTTGA AGAAGATTGA GGAGGAGGTT 
CAGCAGAAAT GGGAGCAGGA CAAGCTTTTT GAGAATGAAG CCCCAGCCAC CATTGGGGAA 
AATACGAATA AGAACAAGTA CTTTATTACA TTTCCCTACC CATATATGAA TGGGAGACTT 
CACCTCGGCC ATACATTCTG CCTTTCCAAG TGTGAGTTTG CAGTGGGTTA CCAGCGGCTT 
AAAGGAAAGC AGTGTCTTTT CCCATTTGGG CTTCACTGCA CTGGGATGCC TATCAAAGCA 
TGTGCAGACA AGCTGAAGAG AGAAATGGAG CTGTATGGTT ATCCGCCCCA ATTCCCTGAA 
GAAGATGAAG AGGAGGAAGA GCAGAAGAAG ACCTCTGATG ACGTTATCAT CAAGGACAAG 
ACAAAGGGCA AGAAGAGCAA AGCTGTAGCA AAGTCTGGAA GCTCCAAGTT CCAGTGGGAC 
ATCATGAAAT CTCTGGGACT GCAGGATGCT GAAATTGTGA AGTTTGCTGA AGCTGAACAT 
TGGCTGGATT ATTTCCCTCC TCTTGCTGTA GAAGATCTGA AGAGAATGGG TCTAAAGGTG 
GATTGGCGGC GTTCCTTTAT TACCACTGAT GTAAACCCCT TTTACGACTC TTTTGTGAGG 
TGGCAGTTCA TCAATTTGAA AGAGAGAAAG AAGATCAAGT TTGGGAAAAG ATATACAATT 
TACTCTCCTA AAGATGGGCA GCCCTGCATG GATCATGACA GACAGACAGG AGAGGGAGTT 
GGTCCTCAAG AATATACTCT AATCAAGATG AAGGTTGTGG AACCTTACCC AACAAAGCTG 
GGTGCTCTTA AAGGCAAGAC CGTTTTTCTG GTTGCTGCAA CACTGAGGCC TGAAACCATG 
TTTGGTCAGA CGAATTGCTG GATTCGTCCA GACATGAAGT ACGTTGTGTT TGAGACCGCC 
AATGGTGACT TGTTCATCAG CACTCAGCGA TCAGCCAGGA ACATGTCCTA CCAAGGCTTT 
ACCAAAGAGA ATGGAGTGGT TCCTGTGATC ATGAACATCA TGGGACAGGA CATCCTTGGC 
TGTGCGTTGA GCGCTCCTCT CACCTCATAC AAGACCATTT ATGCCCTGCC AATGCTCACC 
ATTAAGGAAG ACAAAGGAAC TGGAGTCGTC ACCAGTGTGC CGTCAGATGC ACCGGATGAT 
ATTGCAGCTC TAAGGGACAT TAAAAAGAAA CAGGCATTGA GAGAGAAGTA TGGAATCCAA 
GATTTTATGG TGCTACCATT TGAGCCGGTT CCCATCATTG AAATTCCAGG GTATGGGAAT 
CTGTCAGCTC CACTGGTGTG CGATGAACTG AAGATCCAAA GCCAGAATGA CAGAGAGAAA 
CTGGCCGAGG CTAAGGAGAA GGTCTACCTC AAGGGTTTTT ACGAAGGGAT CATGTTGGTG 
GAAGGTTTCA AAGGACAGAA GGTCCAGGAT GTGAAGAAGC CCATTCAGAA AATGATGGTG 
GAAAAGGGTG AAGCACTGAT TTACATGGAG CCTGAGAAGC AGGTGTTATC TCGGTCTGCA 
GACGAGTGTG TTGTGGCTTT GTGTGACCAG TGGTATCTGG ATTATGGCAA TGAGGAATGG 
AAAAAGCAGG CTCACGGTGT CCTGGAGCAT CTGGAGACGT TCTGTGATGA AACCAGAAAG 
AACTTTGAAG CAACGTTAGC TTGGCTTCAG GAGCACGCCT GTTCCAGAAC GTATGGATTA 
GGAACCCGGT TGCCATGGAA TGAACAATGG CTCATTGAGT CCCTGTCCGA CTCCACCATC 
TATATGGCCT ACTACACCGT GGCACATCTC CTCCAGGGAG GGGTCCTCAG TGGACAAGGA 
CCTTCGCCAC TGGGAATCAG ACCCCAGCAG ATGACGAGGG AGGTGTGGGA TTACATCTTC 
TTCAAAACAT CACCTTTCCC AAAGACGGAC ATCCCGAAGG AGCATCTGCT GAAGCTCAGA 
CGGGAGTTTG AGTTCTGGTA CCCTGTAGAC GTCAGAGTAT CGGGAAAAGA TCTGGTGCCA 
AACCATCTCT CCTATTATCT CTACAATCAT GTAGCCATGT GGCCCAATGA CAGTGGAAAA 
TGGCCGCAAG CTGTACGTGC CAATGGTCAT CTTTTGCTAA ACTCTGAAAA GATGTCCAAA 
TCCACTGGCA ACTTTCTCAC TTTGAGTCAA GCCATTGCCA AGTTTTCAGC AGATGGTATG 
CGTTTGGCTT TGGCTGATGC GGGAGACACT GTGGAGGATG CTAACTTTGT TGAGGCTATG 
GCGGATGCGG GAATACTGCG TCTCTTTACT TGGGTGGAAT GGGTGAAGGA GATGATCGCT 
AATCAGGACA ACCTAAGGAC CGGACCTGCA GACACATTTA ATGACAGAGT GTTTATAAGT 
GAAATGAACT CCGGCATCAT TAAAACAGAT CAGCACTATG AGAGAATGAT GTACAAGGAG 
GCATTGAAGA GTGGCTTCTT TGAATTCCAG GCTGCCAAGG ATAAATACAG GGAGCTGGCC 
ATCGAGGGCA TGCACAAAGA TCTCGTGTTT CAGTTTATCG AGAACCAAAC ACTCCTGCTG 
GCTCCCATCT GCCCACATCT GTGTGAACAC ACCTGGTCCC TGTTGGGAAA GAGCAGTACT 
GTAATGAAGG CTCAATGGCC TACAGCTGGT CCAGTGGACG AGATCTTGAT TCGATCTTCA 
CAGTATCTGA TGGACACTGC CCATGACCTC CGCCTTCGCC TGAAAGCCTA CACACAGCCA 
GCCAAGGGCA AGAAGGGGGA CAACAAACCA CCAGCTAAGC CCACGCACTG CACCATCTAT 
GTTGCAAAGA CCTACCCACC CTGGCAGCAC AGCGCCCTCT CTCTGCTTGG CAAACATTAC 
AAGAGCAACA ATGGAGCTTT ACCTGACAAT AAGGTTATCG CCATGGAGCT CGGAGCCATG 
CCTGAGCTGA AGAAATACAT GAAGAGAGTC ATGCCATTTG TGGCTATGAT AAAGGACAAC 
CTAGAGAAAA ACGGGCCCAG GGTTCTTGAC CTAGAACTGG AGTTCGATGA GCGTGCTGTT 
CTGCTGGAAA ACATTGTTTA TCTGACTAAC TCACTTGAGT TGGATCAGAT AGACGTTGTG 
TTTGCCTCTG AAGCGGATGA GAAAGTAAAG GAGGACTGTT GCCCTGGGAA ACCGTTCTGT 
GTCTTCAGAT CTGAGCCTGG AGTGATAATC TCACTGGTGA ACCCTCAGCC ATCCAACGGT 
CTGTTCTCCA CCAAGATAGA CATCCGACAG GGAGACAGCA AAGACAGCAT CATTCGCAGA 
CTGTCCCGAG TCAACAGAGG CATCAAGGAT TTGTCCAAAG TGAAGCTGAT GCGTTATGAG 
GATCCTGTTC TCGGACCACG GAGAATCCCA GTTTTGGGGA AGGAAGAAGA AGGCAAACTT 
CCAGTCTCGA ACAGTTCAGT CTTCCACATC AGCTTGCAAG AAAGCAAAGT TCATATGAGC 
GACAACGGCC TGAAAGTGGA CATTGGAGAT ACGTTAATCT ACCTGGTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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larsb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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