mut cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mut
Entrez Gene ID	569581
Full Name	methylmalonyl CoA mutase
General protein information	Preferred Names methylmalonyl CoA mutase Names methylmalonyl-CoA mutase, mitochondrial methylmalonyl Coenzyme A mutase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001099226.1	NP_001092696.1	methylmalonyl-CoA mutase, mitochondrial
XM_021468503.1	XP_021324178.1	methylmalonyl-CoA mutase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mut	XP_006244706.1
Gallus gallus (chicken)	MUT	XP_004935974.1
Danio rerio (zebrafish)	mut	NP_001092696.1
Caenorhabditis elegans (roundworm)	mmcm-1	NP_497786.2
Mus musculus (house mouse)	Mut	NP_032676.2
Canis lupus familiaris (dog)	MUT	XP_532164.3
Pan troglodytes (chimpanzee)	MUT	XP_001146876.1
Macaca mulatta (Rhesus monkey)	MUT	XP_001104531.1
Homo sapiens (human)	MUT	NP_000246.2
Bos taurus (cattle)	MUT	NP_776364.2
Xenopus tropicalis (tropical clawed frog)	mut	XP_004915010.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

Expressed sequence tag analysis of expression profiles of zebrafish testis and ovary.
Zeng S, Gong Z
Gene294(1-2)45-53(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mut gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mut cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02833	NM_001099226.1 Latest version!	Danio rerio methylmalonyl CoA mutase (mut), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa02833	XM_021468503.1 Latest version!	Danio rerio methylmalonyl CoA mutase (mut), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mut ( NM_001099226.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001092696.1 Danio rerio methylmalonyl CoA mutase (mut), mRNA.

Price & Availability↑

CloneID	ODa02833
Clone ID Related Accession (Same CDS sequence)	NM_001099226.1 , XM_021468503.1
Accession Version	NM_001099226.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548432000000
Organism	Danio rerio(zebrafish)
Product	methylmalonyl-CoA mutase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC139860.1. On Mar 21, 2008 this sequence version replaced XR_028922.1. ##Evidence-Data-START## Transcript exon combination :: BC139860.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTTGACTG CACAGAAGGC ATGCATCTCT TTGACAGCAA GACGTCTGCT GGGAGCGTCT 
ATATCGTCGA CTCTTGATCC CCAGCAGCGC ACAAGACAGT TCAGCACTGC TGTCCCACTC 
CGAGATGAAG TGAAGATCCA TGAGGCCTGG GCTGCTCTAG CCAAAAAGCA GCTAAAAGGC 
AAAAACCCAG AGGATCTGAT CTGGAAGACC CCTGAAGGCA TCTCTATTAA ACCTGTATAT 
ACTCAGACGG ACACTGCCAA GGCTCCCGAT GAGCTGCCTG GAGTCTTTCC CTTTACCCGG 
GGGCCATATC CAACCATGTA CACCTACAGG CCCTGGACTA TCAGGCAATA TGCTGGATTC 
AGCACTGTGG AAGAAAGCAA CAAGTTTTAC AGGGATAATA TTAAAGCGGG CCAGCAGGGT 
CTGTCTGTGG CTTTTGACCT CGCAACGCAC AGAGGTTACG ACTCTGACAA TCCCAGAGTT 
CATGGCGATG TGGGTATGGC GGGTGTAGCC ATCGACACCG TAGAGGACAC CAAACTCCTG 
TTCGACGGCA TCCCACTGGA GAAGATGTCC GTATCGATGA CAATGAACGG AGCCGTCATT 
CCCATTCTAG CCATGTTCAT CGTGACTGGA GAAGAGCAAG GTGTGCCGAA GGAGAAGCTG 
ACGGGCACCA TCCAGAATGA CATCCTCAAA GAGTTCATGG TGCGAAACAC ATACATCTTC 
CCTCCAGAGC CCTCAATGCA CGCCATCGCA GATATCTTCG CCTACACTTC AAAGAATATG 
CCCAAGTTTA ATTCTATTTC CATCAGTGGA TACCACATAC AGGAAGCTGG TGCTGATGCC 
ATCCTGGAGT TGGCTTACAC GATTGCCAAT GGACTGGAGT ACTGCAGGAC TGGACTAAAG 
GCTGGACTCA CTATTGATGA ATTTGCACCC AGATTGTCTT TCTTCTGGGG CATCGGGATG 
AACTTTTATA TGGAAATAGC GAAGCTGAGA GCAGCACGAC GTCTGTGGGC GACACTCATC 
AAAGAGAAGT TTCAGCCGAA GAACTCCAAA TCCCTCCTGC TGAGAACTCA CTGTCAGACA 
TCTGGATGGT CCCTTACTGA GCAGGACCCT TACAACAACG TGATCCGTAC CGTAATCGAG 
GCCATGGCTG CTGTGTTTGG AGGCACTCAG TCTTTGCACA CCAATTCTTT TGACGAAGCT 
CTGGGTTTGC CCACGGTCAA GAGCGCCCGC ATCGCCCGCA ATACCCAGAT CATCATCCAG 
GAGGAGTCAG GCATCCCAAA GGTGGCTGAT CCCTGGGGTG GATCCTACAT GATGGAGGCT 
CTCACCAATG ACGTCTACAA CTCGGCCCTC AAGTTTATCA CAGAGATTGA GGACATGGGA 
GGTATGGCTC GTGCTGTAGC CGAAGGAATT CCCAAACTGC GCATCGAGGA GTGCGCTGCC 
CGAAGACAAG CACGCATTGA TTCAGGCTCT GAGGTCATCG TGGGGGTCAA CAAATACCGC 
CTGGAGAAGG AAGAATCTGT CGACGTGCTT GCCATCGACA ACACAGCTGT GCGTAACAAG 
CAGATTGAAA AGCTGCAGAA GGTCAGAGAG AGCAGGGATG CAGAGGCGGC AAAGAGATGT 
TTGGCTGCGA TCGAACAGTG TGCCCGAACC AGAGATGGAA ATCTTCTTGA ACTTGCCGTG 
GAGGCTGCCA GAGCACGGTG CTCGGTTGGA GAGATCACAG ATGCCATGAA ATCAGTTTTC 
GGGGAGCACA AGGCCAGCAC TCGCATGGTG AGCGGAGCAT ATCGCAGCGA GTTCGGCGAA 
CACGAGGAGA TAGCACTGGC GCATAACAGA GTTGTTCAGT TTAAAAAGCA TGAAGGCAGA 
AATCCTCGTC TGCTAGTGGC CAAAATGGGA CAGGATGGTC ATGATAGAGG GGCAAAGGTC 
ATTGCCACTG GATTTGCAGA TCTTGGGTTC GATGTGGACG TTGGCCCACT TTTCCAGACC 
CCGCTGGAGG TGGCCCAACA GGCGGTGGAT GCAGACGTTC ACTGTGTAGG GGTCAGCACA 
TTGGCAGCTG GACATAAGAC CCTCGTGCCC GAGCTCATCA AAGAGCTGCG TAACCTGAAC 
CGGCCCGATA TCATGGTGAT CTGCGGAGGG GTCATACCAC CACAGGACTA TGAGTTCCTC 
TATGCGAGTG GTGTCAGCTG TATCTTCGGC CCCGGGACAA GGATTCCAAA AGCAGCCGTG 
GAGGTTCTTG ACAGCATTGA AAAAAGCCTG GAGAAGAACC GGCAGGCCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092696.1
CDS	87..2360
Translation	MLTAQKACISLTARRLLGASISSTLDPQQRTRQFSTAVPLRDEVKIHEAWAALAKKQLKGKNPEDLIWKTPEGISIKPVYTQTDTAKAPDELPGVFPFTRGPYPTMYTYRPWTIRQYAGFSTVEESNKFYRDNIKAGQQGLSVAFDLATHRGYDSDNPRVHGDVGMAGVAIDTVEDTKLLFDGIPLEKMSVSMTMNGAVIPILAMFIVTGEEQGVPKEKLTGTIQNDILKEFMVRNTYIFPPEPSMHAIADIFAYTSKNMPKFNSISISGYHIQEAGADAILELAYTIANGLEYCRTGLKAGLTIDEFAPRLSFFWGIGMNFYMEIAKLRAARRLWATLIKEKFQPKNSKSLLLRTHCQTSGWSLTEQDPYNNVIRTVIEAMAAVFGGTQSLHTNSFDEALGLPTVKSARIARNTQIIIQEESGIPKVADPWGGSYMMEALTNDVYNSALKFITEIEDMGGMARAVAEGIPKLRIEECAARRQARIDSGSEVIVGVNKYRLEKEESVDVLAIDNTAVRNKQIEKLQKVRESRDAEAAKRCLAAIEQCARTRDGNLLELAVEAARARCSVGEITDAMKSVFGEHKASTRMVSGAYRSEFGEHEEIALAHNRVVQFKKHEGRNPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDVGPLFQTPLEVAQQAVDADVHCVGVSTLAAGHKTLVPELIKELRNLNRPDIMVICGGVIPPQDYEFLYASGVSCIFGPGTRIPKAAVEVLDSIEKSLEKNRQAM

Target ORF information:

RefSeq Version	NM_001099226.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methylmalonyl CoA mutase (mut), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099226.1

ORF Insert Sequence:

ATGTTGACTG CACAGAAGGC ATGCATCTCT TTGACAGCAA GACGTCTGCT GGGAGCGTCT 
ATATCGTCGA CTCTTGATCC CCAGCAGCGC ACAAGACAGT TCAGCACTGC TGTCCCACTC 
CGAGATGAAG TGAAGATCCA TGAGGCCTGG GCTGCTCTAG CCAAAAAGCA GCTAAAAGGC 
AAAAACCCAG AGGATCTGAT CTGGAAGACC CCTGAAGGCA TCTCTATTAA ACCTGTATAT 
ACTCAGACGG ACACTGCCAA GGCTCCCGAT GAGCTGCCTG GAGTCTTTCC CTTTACCCGG 
GGGCCATATC CAACCATGTA CACCTACAGG CCCTGGACTA TCAGGCAATA TGCTGGATTC 
AGCACTGTGG AAGAAAGCAA CAAGTTTTAC AGGGATAATA TTAAAGCGGG CCAGCAGGGT 
CTGTCTGTGG CTTTTGACCT CGCAACGCAC AGAGGTTACG ACTCTGACAA TCCCAGAGTT 
CATGGCGATG TGGGTATGGC GGGTGTAGCC ATCGACACCG TAGAGGACAC CAAACTCCTG 
TTCGACGGCA TCCCACTGGA GAAGATGTCC GTATCGATGA CAATGAACGG AGCCGTCATT 
CCCATTCTAG CCATGTTCAT CGTGACTGGA GAAGAGCAAG GTGTGCCGAA GGAGAAGCTG 
ACGGGCACCA TCCAGAATGA CATCCTCAAA GAGTTCATGG TGCGAAACAC ATACATCTTC 
CCTCCAGAGC CCTCAATGCA CGCCATCGCA GATATCTTCG CCTACACTTC AAAGAATATG 
CCCAAGTTTA ATTCTATTTC CATCAGTGGA TACCACATAC AGGAAGCTGG TGCTGATGCC 
ATCCTGGAGT TGGCTTACAC GATTGCCAAT GGACTGGAGT ACTGCAGGAC TGGACTAAAG 
GCTGGACTCA CTATTGATGA ATTTGCACCC AGATTGTCTT TCTTCTGGGG CATCGGGATG 
AACTTTTATA TGGAAATAGC GAAGCTGAGA GCAGCACGAC GTCTGTGGGC GACACTCATC 
AAAGAGAAGT TTCAGCCGAA GAACTCCAAA TCCCTCCTGC TGAGAACTCA CTGTCAGACA 
TCTGGATGGT CCCTTACTGA GCAGGACCCT TACAACAACG TGATCCGTAC CGTAATCGAG 
GCCATGGCTG CTGTGTTTGG AGGCACTCAG TCTTTGCACA CCAATTCTTT TGACGAAGCT 
CTGGGTTTGC CCACGGTCAA GAGCGCCCGC ATCGCCCGCA ATACCCAGAT CATCATCCAG 
GAGGAGTCAG GCATCCCAAA GGTGGCTGAT CCCTGGGGTG GATCCTACAT GATGGAGGCT 
CTCACCAATG ACGTCTACAA CTCGGCCCTC AAGTTTATCA CAGAGATTGA GGACATGGGA 
GGTATGGCTC GTGCTGTAGC CGAAGGAATT CCCAAACTGC GCATCGAGGA GTGCGCTGCC 
CGAAGACAAG CACGCATTGA TTCAGGCTCT GAGGTCATCG TGGGGGTCAA CAAATACCGC 
CTGGAGAAGG AAGAATCTGT CGACGTGCTT GCCATCGACA ACACAGCTGT GCGTAACAAG 
CAGATTGAAA AGCTGCAGAA GGTCAGAGAG AGCAGGGATG CAGAGGCGGC AAAGAGATGT 
TTGGCTGCGA TCGAACAGTG TGCCCGAACC AGAGATGGAA ATCTTCTTGA ACTTGCCGTG 
GAGGCTGCCA GAGCACGGTG CTCGGTTGGA GAGATCACAG ATGCCATGAA ATCAGTTTTC 
GGGGAGCACA AGGCCAGCAC TCGCATGGTG AGCGGAGCAT ATCGCAGCGA GTTCGGCGAA 
CACGAGGAGA TAGCACTGGC GCATAACAGA GTTGTTCAGT TTAAAAAGCA TGAAGGCAGA 
AATCCTCGTC TGCTAGTGGC CAAAATGGGA CAGGATGGTC ATGATAGAGG GGCAAAGGTC 
ATTGCCACTG GATTTGCAGA TCTTGGGTTC GATGTGGACG TTGGCCCACT TTTCCAGACC 
CCGCTGGAGG TGGCCCAACA GGCGGTGGAT GCAGACGTTC ACTGTGTAGG GGTCAGCACA 
TTGGCAGCTG GACATAAGAC CCTCGTGCCC GAGCTCATCA AAGAGCTGCG TAACCTGAAC 
CGGCCCGATA TCATGGTGAT CTGCGGAGGG GTCATACCAC CACAGGACTA TGAGTTCCTC 
TATGCGAGTG GTGTCAGCTG TATCTTCGGC CCCGGGACAA GGATTCCAAA AGCAGCCGTG 
GAGGTTCTTG ACAGCATTGA AAAAAGCCTG GAGAAGAACC GGCAGGCCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mut ( XM_021468503.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324178.1 Danio rerio methylmalonyl CoA mutase (mut), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa02833
Clone ID Related Accession (Same CDS sequence)	NM_001099226.1 , XM_021468503.1
Accession Version	XM_021468503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	methylmalonyl-CoA mutase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGACTG CACAGAAGGC ATGCATCTCT TTGACAGCAA GACGTCTGCT GGGAGCGTCT 
ATATCGTCGA CTCTTGATCC CCAGCAGCGC ACAAGACAGT TCAGCACTGC TGTCCCACTC 
CGAGATGAAG TGAAGATCCA TGAGGCCTGG GCTGCTCTAG CCAAAAAGCA GCTAAAAGGC 
AAAAACCCAG AGGATCTGAT CTGGAAGACC CCTGAAGGCA TCTCTATTAA ACCTGTATAT 
ACTCAGACGG ACACTGCCAA GGCTCCCGAT GAGCTGCCTG GAGTCTTTCC CTTTACCCGG 
GGGCCATATC CAACCATGTA CACCTACAGG CCCTGGACTA TCAGGCAATA TGCTGGATTC 
AGCACTGTGG AAGAAAGCAA CAAGTTTTAC AGGGATAATA TTAAAGCGGG CCAGCAGGGT 
CTGTCTGTGG CTTTTGACCT CGCAACGCAC AGAGGTTACG ACTCTGACAA TCCCAGAGTT 
CATGGCGATG TGGGTATGGC GGGTGTAGCC ATCGACACCG TAGAGGACAC CAAACTCCTG 
TTCGACGGCA TCCCACTGGA GAAGATGTCC GTATCGATGA CAATGAACGG AGCCGTCATT 
CCCATTCTAG CCATGTTCAT CGTGACTGGA GAAGAGCAAG GTGTGCCGAA GGAGAAGCTG 
ACGGGCACCA TCCAGAATGA CATCCTCAAA GAGTTCATGG TGCGAAACAC ATACATCTTC 
CCTCCAGAGC CCTCAATGCA CGCCATCGCA GATATCTTCG CCTACACTTC AAAGAATATG 
CCCAAGTTTA ATTCTATTTC CATCAGTGGA TACCACATAC AGGAAGCTGG TGCTGATGCC 
ATCCTGGAGT TGGCTTACAC GATTGCCAAT GGACTGGAGT ACTGCAGGAC TGGACTAAAG 
GCTGGACTCA CTATTGATGA ATTTGCACCC AGATTGTCTT TCTTCTGGGG CATCGGGATG 
AACTTTTATA TGGAAATAGC GAAGCTGAGA GCAGCACGAC GTCTGTGGGC GACACTCATC 
AAAGAGAAGT TTCAGCCGAA GAACTCCAAA TCCCTCCTGC TGAGAACTCA CTGTCAGACA 
TCTGGATGGT CCCTTACTGA GCAGGACCCT TACAACAACG TGATCCGTAC CGTAATCGAG 
GCCATGGCTG CTGTGTTTGG AGGCACTCAG TCTTTGCACA CCAATTCTTT TGACGAAGCT 
CTGGGTTTGC CCACGGTCAA GAGCGCCCGC ATCGCCCGCA ATACCCAGAT CATCATCCAG 
GAGGAGTCAG GCATCCCAAA GGTGGCTGAT CCCTGGGGTG GATCCTACAT GATGGAGGCT 
CTCACCAATG ACGTCTACAA CTCGGCCCTC AAGTTTATCA CAGAGATTGA GGACATGGGA 
GGTATGGCTC GTGCTGTAGC CGAAGGAATT CCCAAACTGC GCATCGAGGA GTGCGCTGCC 
CGAAGACAAG CACGCATTGA TTCAGGCTCT GAGGTCATCG TGGGGGTCAA CAAATACCGC 
CTGGAGAAGG AAGAATCTGT CGACGTGCTT GCCATCGACA ACACAGCTGT GCGTAACAAG 
CAGATTGAAA AGCTGCAGAA GGTCAGAGAG AGCAGGGATG CAGAGGCGGC AAAGAGATGT 
TTGGCTGCGA TCGAACAGTG TGCCCGAACC AGAGATGGAA ATCTTCTTGA ACTTGCCGTG 
GAGGCTGCCA GAGCACGGTG CTCGGTTGGA GAGATCACAG ATGCCATGAA ATCAGTTTTC 
GGGGAGCACA AGGCCAGCAC TCGCATGGTG AGCGGAGCAT ATCGCAGCGA GTTCGGCGAA 
CACGAGGAGA TAGCACTGGC GCATAACAGA GTTGTTCAGT TTAAAAAGCA TGAAGGCAGA 
AATCCTCGTC TGCTAGTGGC CAAAATGGGA CAGGATGGTC ATGATAGAGG GGCAAAGGTC 
ATTGCCACTG GATTTGCAGA TCTTGGGTTC GATGTGGACG TTGGCCCACT TTTCCAGACC 
CCGCTGGAGG TGGCCCAACA GGCGGTGGAT GCAGACGTTC ACTGTGTAGG GGTCAGCACA 
TTGGCAGCTG GACATAAGAC CCTCGTGCCC GAGCTCATCA AAGAGCTGCG TAACCTGAAC 
CGGCCCGATA TCATGGTGAT CTGCGGAGGG GTCATACCAC CACAGGACTA TGAGTTCCTC 
TATGCGAGTG GTGTCAGCTG TATCTTCGGC CCCGGGACAA GGATTCCAAA AGCAGCCGTG 
GAGGTTCTTG ACAGCATTGA AAAAAGCCTG GAGAAGAACC GGCAGGCCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324178.1
CDS	77..2350
Translation	MLTAQKACISLTARRLLGASISSTLDPQQRTRQFSTAVPLRDEVKIHEAWAALAKKQLKGKNPEDLIWKTPEGISIKPVYTQTDTAKAPDELPGVFPFTRGPYPTMYTYRPWTIRQYAGFSTVEESNKFYRDNIKAGQQGLSVAFDLATHRGYDSDNPRVHGDVGMAGVAIDTVEDTKLLFDGIPLEKMSVSMTMNGAVIPILAMFIVTGEEQGVPKEKLTGTIQNDILKEFMVRNTYIFPPEPSMHAIADIFAYTSKNMPKFNSISISGYHIQEAGADAILELAYTIANGLEYCRTGLKAGLTIDEFAPRLSFFWGIGMNFYMEIAKLRAARRLWATLIKEKFQPKNSKSLLLRTHCQTSGWSLTEQDPYNNVIRTVIEAMAAVFGGTQSLHTNSFDEALGLPTVKSARIARNTQIIIQEESGIPKVADPWGGSYMMEALTNDVYNSALKFITEIEDMGGMARAVAEGIPKLRIEECAARRQARIDSGSEVIVGVNKYRLEKEESVDVLAIDNTAVRNKQIEKLQKVRESRDAEAAKRCLAAIEQCARTRDGNLLELAVEAARARCSVGEITDAMKSVFGEHKASTRMVSGAYRSEFGEHEEIALAHNRVVQFKKHEGRNPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDVGPLFQTPLEVAQQAVDADVHCVGVSTLAAGHKTLVPELIKELRNLNRPDIMVICGGVIPPQDYEFLYASGVSCIFGPGTRIPKAAVEVLDSIEKSLEKNRQAM

Target ORF information:

RefSeq Version	XM_021468503.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methylmalonyl CoA mutase (mut), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468503.1

ORF Insert Sequence:

ATGTTGACTG CACAGAAGGC ATGCATCTCT TTGACAGCAA GACGTCTGCT GGGAGCGTCT 
ATATCGTCGA CTCTTGATCC CCAGCAGCGC ACAAGACAGT TCAGCACTGC TGTCCCACTC 
CGAGATGAAG TGAAGATCCA TGAGGCCTGG GCTGCTCTAG CCAAAAAGCA GCTAAAAGGC 
AAAAACCCAG AGGATCTGAT CTGGAAGACC CCTGAAGGCA TCTCTATTAA ACCTGTATAT 
ACTCAGACGG ACACTGCCAA GGCTCCCGAT GAGCTGCCTG GAGTCTTTCC CTTTACCCGG 
GGGCCATATC CAACCATGTA CACCTACAGG CCCTGGACTA TCAGGCAATA TGCTGGATTC 
AGCACTGTGG AAGAAAGCAA CAAGTTTTAC AGGGATAATA TTAAAGCGGG CCAGCAGGGT 
CTGTCTGTGG CTTTTGACCT CGCAACGCAC AGAGGTTACG ACTCTGACAA TCCCAGAGTT 
CATGGCGATG TGGGTATGGC GGGTGTAGCC ATCGACACCG TAGAGGACAC CAAACTCCTG 
TTCGACGGCA TCCCACTGGA GAAGATGTCC GTATCGATGA CAATGAACGG AGCCGTCATT 
CCCATTCTAG CCATGTTCAT CGTGACTGGA GAAGAGCAAG GTGTGCCGAA GGAGAAGCTG 
ACGGGCACCA TCCAGAATGA CATCCTCAAA GAGTTCATGG TGCGAAACAC ATACATCTTC 
CCTCCAGAGC CCTCAATGCA CGCCATCGCA GATATCTTCG CCTACACTTC AAAGAATATG 
CCCAAGTTTA ATTCTATTTC CATCAGTGGA TACCACATAC AGGAAGCTGG TGCTGATGCC 
ATCCTGGAGT TGGCTTACAC GATTGCCAAT GGACTGGAGT ACTGCAGGAC TGGACTAAAG 
GCTGGACTCA CTATTGATGA ATTTGCACCC AGATTGTCTT TCTTCTGGGG CATCGGGATG 
AACTTTTATA TGGAAATAGC GAAGCTGAGA GCAGCACGAC GTCTGTGGGC GACACTCATC 
AAAGAGAAGT TTCAGCCGAA GAACTCCAAA TCCCTCCTGC TGAGAACTCA CTGTCAGACA 
TCTGGATGGT CCCTTACTGA GCAGGACCCT TACAACAACG TGATCCGTAC CGTAATCGAG 
GCCATGGCTG CTGTGTTTGG AGGCACTCAG TCTTTGCACA CCAATTCTTT TGACGAAGCT 
CTGGGTTTGC CCACGGTCAA GAGCGCCCGC ATCGCCCGCA ATACCCAGAT CATCATCCAG 
GAGGAGTCAG GCATCCCAAA GGTGGCTGAT CCCTGGGGTG GATCCTACAT GATGGAGGCT 
CTCACCAATG ACGTCTACAA CTCGGCCCTC AAGTTTATCA CAGAGATTGA GGACATGGGA 
GGTATGGCTC GTGCTGTAGC CGAAGGAATT CCCAAACTGC GCATCGAGGA GTGCGCTGCC 
CGAAGACAAG CACGCATTGA TTCAGGCTCT GAGGTCATCG TGGGGGTCAA CAAATACCGC 
CTGGAGAAGG AAGAATCTGT CGACGTGCTT GCCATCGACA ACACAGCTGT GCGTAACAAG 
CAGATTGAAA AGCTGCAGAA GGTCAGAGAG AGCAGGGATG CAGAGGCGGC AAAGAGATGT 
TTGGCTGCGA TCGAACAGTG TGCCCGAACC AGAGATGGAA ATCTTCTTGA ACTTGCCGTG 
GAGGCTGCCA GAGCACGGTG CTCGGTTGGA GAGATCACAG ATGCCATGAA ATCAGTTTTC 
GGGGAGCACA AGGCCAGCAC TCGCATGGTG AGCGGAGCAT ATCGCAGCGA GTTCGGCGAA 
CACGAGGAGA TAGCACTGGC GCATAACAGA GTTGTTCAGT TTAAAAAGCA TGAAGGCAGA 
AATCCTCGTC TGCTAGTGGC CAAAATGGGA CAGGATGGTC ATGATAGAGG GGCAAAGGTC 
ATTGCCACTG GATTTGCAGA TCTTGGGTTC GATGTGGACG TTGGCCCACT TTTCCAGACC 
CCGCTGGAGG TGGCCCAACA GGCGGTGGAT GCAGACGTTC ACTGTGTAGG GGTCAGCACA 
TTGGCAGCTG GACATAAGAC CCTCGTGCCC GAGCTCATCA AAGAGCTGCG TAACCTGAAC 
CGGCCCGATA TCATGGTGAT CTGCGGAGGG GTCATACCAC CACAGGACTA TGAGTTCCTC 
TATGCGAGTG GTGTCAGCTG TATCTTCGGC CCCGGGACAA GGATTCCAAA AGCAGCCGTG 
GAGGTTCTTG ACAGCATTGA AAAAAGCCTG GAGAAGAACC GGCAGGCCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

Expressed sequence tag analysis of expression profiles of zebrafish testis and ovary.
Gene294(1-2)45-53(2002 Jul)
Zeng S,Gong Z

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mut cDNA ORF clone, Danio rerio(zebrafish)

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