lepr cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lepr
Entrez Gene ID	567241
Full Name	leptin receptor
Synonyms	obr
General protein information	Preferred Names leptin receptor Names leptin receptor
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001309403.1	NP_001296332.1	leptin receptor precursor
XM_021476908.1	XP_021332583.1	leptin receptor isoform X1
XM_021476907.1	XP_021332582.1	leptin receptor isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Lepr	NP_666258.2
Rattus norvegicus (Norway rat)	Lepr	NP_036728.1
Xenopus tropicalis (tropical clawed frog)	lepr	NP_001037866.2
Macaca mulatta (Rhesus monkey)	LEPR	NP_001027991.1
Danio rerio (zebrafish)	lepr	NP_001106847.1
Gallus gallus (chicken)	LEPR	NP_989654.1
Homo sapiens (human)	LEPR	NP_002294.2
Pan troglodytes (chimpanzee)	LEPR	XP_001161937.1
Canis lupus familiaris (dog)	LEPR	NP_001019805.1
Bos taurus (cattle)	LEPR	NP_001012285.2

Gene Ontology
Bibliography

Related articles in PubMed

Examining the responses of the zebrafish (Danio rerio) gastrointestinal system to the suspected obesogen diethylhexyl phthalate.
Buerger AN, Schmidt J, Chase A, Paixao C, Patel TN, Brumback BA, Kane AS, Martyniuk CJ, Bisesi JH Jr
Environmental pollution (Barking, Essex : 1987)2451086-1094(2019 Feb)

Leptin induces muscle wasting in a zebrafish
Yang Q, Yan C, Wang X, Gong Z
Disease models & mechanisms12(2)(2019 02)

Neuroinflammatory signals drive spinal curve formation in zebrafish models of idiopathic scoliosis.
Van Gennip JLM, Boswell CW, Ciruna B
Science advances4(12)eaav1781(2018 12)

Histone Deacetylase-Mediated Müller Glia Reprogramming through Her4.1-Lin28a Axis Is Essential for Retina Regeneration in Zebrafish.
Mitra S, Sharma P, Kaur S, Khursheed MA, Gupta S, Ahuja R, Kurup AJ, Chaudhary M, Ramachandran R
iScience768-84(2018 Sep)

Huang P, Xiong S, Kang J, Mei J, Gui JF
Frontiers in physiology9676(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following lepr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lepr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa11917	NM_001309403.1 Latest version!	Danio rerio leptin receptor (lepr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa11917	XM_021476908.1 Latest version!	Danio rerio leptin receptor (lepr), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa11917	XM_021476907.1 Latest version!	Danio rerio leptin receptor (lepr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lepr ( NM_001309403.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001296332.1 Danio rerio leptin receptor (lepr), mRNA.

Price & Availability↑

CloneID	ODa11917
Clone ID Related Accession (Same CDS sequence)	NM_001309403.1 , XM_021476908.1 , XM_021476907.1
Accession Version	NM_001309403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1563552000000
Organism	Danio rerio(zebrafish)
Product	leptin receptor precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX936440.20. On May 30, 2015 this sequence version replaced XM_005165878.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ007541.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGATGTCTG TCTTCATCAT GCTTGCACTG CTTGTGATTT TTATTGCCGT TTCACAAGGC 
CTTGCAGATT TGAATCCTTC TGATGGAGTC AGCGATGGTG TTTACGAAGA CCTGAAGTGG 
AAAGCTCTCC TGTGTTGTGA TCATCCTGTT CAGACGCTTA ACAGTGGTCT TTCAGAACAC 
CCACCCGAAC AACACTGCCA ACTGCTAAAT GCTACAAAAC AACAGTCTTT TGGAAGCTGT 
TTGGACATCC TGTGCTGGCT TGAAGGTGAA CGGGAAAATT TGATTTGTAA TGCAAAGACC 
CGTAGAGCAG CAGCAGCTTC CACTCTGGTC AGCGTCAGCC CTCATCAATT AGTTGTACAG 
ATGGATGTAC ATTCAGATGA AGCAAATTCC ACTGCTCAGT GTGTTGGAGA AGAGACTGCC 
ATCTGCTCCG TTTCTCTTCA CGGCGGGGAT GCAACAGTCT CATTAGTTAT AATTATATCT 
GAGAATGGGA CCACTGCACA ATCACAGAAG ATGCAAGTCA GCACATATGA ACTACAAAGA 
CCTGGCGCAC CTGTCAATCT GCATTACAAT GTGACGACAG AAGGGGAAGT TATATTCAGG 
TGGACCGGCA CACAACCTGA CAACAACACT ATAAACTATG AGATCCAATA CTCATCCAAT 
TCCTTGCTTC AGCAGTGGAA GGTATTGAAG GTCAAAGGTC GTTCATGGGT TGCTCTGAAT 
GAGCTCAGTT CTGACATCAG ATACACGGTT CAAGTGCGCT GCCAAAACCA TCTCGGCTAC 
TGGAGTGAAT GGAGCCAACC TTTCTATTTC AAACTAGATG TCAGTTACAT CCCTGCTGAA 
GTCTTCACAA CGCAGGAGTC AGAGGTGACA GTTTACTCTG TGTTCCACAA CCGCAGCTGG 
AGCGCCAGTA AAGCCGTGTG GTTTCTGAAC GGCAAGATGA AGATACCAGA AAGCCAGTAC 
AGAGTCATCA ATGAGCAGGT CAGCACAGTC ACTCTAAAGA TGGACAAAGC AGGATTTGAC 
ACTCTGATGT GCTGCCTCAC ATTGGGAGAG AAATCTATGT GCTCCATCGC CTATGCCAAA 
ATATACACAG AAGGAAGGTT TAACGCAAAT ATTACCTGCG AAAGCGAGTA TTCATATGTG 
GACACCATGA TCTGTAAGTG GAATCAGAGC AATTGGGCAC AGGCCAGACT CCTTTACAGA 
CAATACAGAT CAAAGTGCAC TGAAGAAGCT GAAGAAGACA CGTCTTTGGT GAAAGAGTGT 
CCATCTAAAG CAGGAGATCA CAGACAGTGC ACCTTAAGCC AAATCAGCAT GATCTTTTGC 
TATAAATTCT GGCTTGAAGT GGAAGGAGGA CGTGGCCAAT CGTTTCCTGT CTACGTTACG 
CCTATTGACT ATGTGAAACC GTCACCTCCC TTTGATCTGG AGGCAATCAC TCTGCCGAGT 
AAAACCCTGA GTGTAAGGTG GAAACGCCCC TCTTTGCCTG TATACGGCAT GCAGTACGAG 
CTGCAATTCA AGGCTTTGGC TGGAATGGCA AATACGCAAT GGAAGGTCAT TGGTCCTCTA 
TTGGAACCAC AGGCAGAGAT TCAGCTTGAG GAGTCCTGTG TTCACTTTAA AGTAGAAGTT 
CGCTGTAAAG ACGTGAACGA CACTGGATAC TGGAGCGACT GGAGCAATAG TCACATTTCC 
ACTGTTTTCA ACTTGAAAGC ACCAGAGATG GGACCAGACT TCTGGCGCAT TTTACAAGAG 
GATCCTACTA GGAATGTGAC AAACGTTACA CTGATCTTTA AGCAGCCCAT TCTAGCAGGA 
GATCCAAATA GCTGCGTGGA GGGTTTAGTG ATTAAACACC AGGCCTCAGG TGGAGTCATG 
TGGTCAAATG AAACAACCTT GGCTCGGTTT CACTCATTCC AGTGGAGGAA GGAAGCGCAT 
ACAGTCACTG TGATGTCTCG CAACGCTTTG GGCATTTCCA CATGGAACAG AAACATCACG 
CTCTTACGTC AAGCCAAACG GCGATGTGTG CGTTCGTTCA GTGCAGTTGC AAATGTGAGC 
TGTGTGCACC TCTCCTGGAG CCTGTTATCT GATCAGCCGG TGCCTCAGTC ATTTGTTATT 
GAATGGTTAG ACCTGAACAA AGACCCCGAA AAGGATGTGT CTCTCACAGA AAGAATACAG 
TGGGTCAGGG TTGAGTCCAG ATCGAGAGAT CTTTCTCTTT GCCGTCGTTT TTATGGCTCA 
GAGGAGTTCA CCTTGTATCC AGTGTTTGCG GATGGTGAAG GGGAACCGGC TCGATACACA 
GCTACAAGGG GTGATCCTGC AGCGTATATA CTGCTACTGA TCATCGCCTT CCTGTCAGTG 
GTGCTGTTTG TCACACTGAT GATGTCACAG AACCAGATGA AAAAACTCAT GTGGAAGGAT 
GTTCCAAATC CCAACAAGTG CTCTTGGGCC AAAGGAATGG ACTTCAGGCA GATTGACACC 
ATGGAGAGCC TGTTCCCTCA TTCAGAGGGT CTCACTGCCT GTCCATTGCT GCTTGTCTCC 
GAGAGCATCT GTGAGGTTGA AATCATCGAG AAACCTCATC CTCTTACCAT TGAAAACGTA 
AAAGACAATG AAGAACTTCC CTCCGGGGAC AAGACAACCA CAGACTCTGG CCTTCAGGGA 
GACTCTTCTG AAGCTCTGGA AGCATCTACT GCTGCTCCCA CTCCAGAAAC ATCAGGCCAG 
TCTTCAGTCA CGTACTCCAC CATCCTCCTC TCCGACCAGC CCAGCCAACT CCAAAAGCAG 
CAGGAGAGTC TCAGCAGCTC CAGTGACGAA GGCAACTTCT CTGCCAACAA CTCGGACATT 
TCCGGCTCCT TCCCTGGAGG ACTTTGGGAC TTGGAGAACA GCAATCCTCG CCATTCCAGC 
TCCTACAACT CTGTGGAGGA GTTTTCGGAA ACATCAGAAC CTGATTATGA AGCTTCGGAA 
AACACAGGTT TAGCTAAAGA CCTCTATTAC CTCGAGATGA CCGGAGAGGA GAAAGAAGAA 
GAAGAAGAAG AAGAAGAAGA AGAAGAAGAA GAAGACGAAC CAGAGGAGGG TCAAAGTAAG 
AATAAAAGAG TGATGGGTGT AAACCCCAGG CCTCTACTGG AAAGCCAAAA TTCCACAGCC 
AGCAATTCCA ATAACATGTC TCACAGCATT CCTCTTTATC TGCCTCAGTT TCGAAGTGAA 
TGTATCAATC CAACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296332.1
CDS	250..3447
Translation	MMSVFIMLALLVIFIAVSQGLADLNPSDGVSDGVYEDLKWKALLCCDHPVQTLNSGLSEHPPEQHCQLLNATKQQSFGSCLDILCWLEGERENLICNAKTRRAAAASTLVSVSPHQLVVQMDVHSDEANSTAQCVGEETAICSVSLHGGDATVSLVIIISENGTTAQSQKMQVSTYELQRPGAPVNLHYNVTTEGEVIFRWTGTQPDNNTINYEIQYSSNSLLQQWKVLKVKGRSWVALNELSSDIRYTVQVRCQNHLGYWSEWSQPFYFKLDVSYIPAEVFTTQESEVTVYSVFHNRSWSASKAVWFLNGKMKIPESQYRVINEQVSTVTLKMDKAGFDTLMCCLTLGEKSMCSIAYAKIYTEGRFNANITCESEYSYVDTMICKWNQSNWAQARLLYRQYRSKCTEEAEEDTSLVKECPSKAGDHRQCTLSQISMIFCYKFWLEVEGGRGQSFPVYVTPIDYVKPSPPFDLEAITLPSKTLSVRWKRPSLPVYGMQYELQFKALAGMANTQWKVIGPLLEPQAEIQLEESCVHFKVEVRCKDVNDTGYWSDWSNSHISTVFNLKAPEMGPDFWRILQEDPTRNVTNVTLIFKQPILAGDPNSCVEGLVIKHQASGGVMWSNETTLARFHSFQWRKEAHTVTVMSRNALGISTWNRNITLLRQAKRRCVRSFSAVANVSCVHLSWSLLSDQPVPQSFVIEWLDLNKDPEKDVSLTERIQWVRVESRSRDLSLCRRFYGSEEFTLYPVFADGEGEPARYTATRGDPAAYILLLIIAFLSVVLFVTLMMSQNQMKKLMWKDVPNPNKCSWAKGMDFRQIDTMESLFPHSEGLTACPLLLVSESICEVEIIEKPHPLTIENVKDNEELPSGDKTTTDSGLQGDSSEALEASTAAPTPETSGQSSVTYSTILLSDQPSQLQKQQESLSSSSDEGNFSANNSDISGSFPGGLWDLENSNPRHSSSYNSVEEFSETSEPDYEASENTGLAKDLYYLEMTGEEKEEEEEEEEEEEEEDEPEEGQSKNKRVMGVNPRPLLESQNSTASNSNNMSHSIPLYLPQFRSECINPT

Target ORF information:

RefSeq Version	NM_001309403.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leptin receptor (lepr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001309403.1

ORF Insert Sequence:

ATGATGTCTG TCTTCATCAT GCTTGCACTG CTTGTGATTT TTATTGCCGT TTCACAAGGC 
CTTGCAGATT TGAATCCTTC TGATGGAGTC AGCGATGGTG TTTACGAAGA CCTGAAGTGG 
AAAGCTCTCC TGTGTTGTGA TCATCCTGTT CAGACGCTTA ACAGTGGTCT TTCAGAACAC 
CCACCCGAAC AACACTGCCA ACTGCTAAAT GCTACAAAAC AACAGTCTTT TGGAAGCTGT 
TTGGACATCC TGTGCTGGCT TGAAGGTGAA CGGGAAAATT TGATTTGTAA TGCAAAGACC 
CGTAGAGCAG CAGCAGCTTC CACTCTGGTC AGCGTCAGCC CTCATCAATT AGTTGTACAG 
ATGGATGTAC ATTCAGATGA AGCAAATTCC ACTGCTCAGT GTGTTGGAGA AGAGACTGCC 
ATCTGCTCCG TTTCTCTTCA CGGCGGGGAT GCAACAGTCT CATTAGTTAT AATTATATCT 
GAGAATGGGA CCACTGCACA ATCACAGAAG ATGCAAGTCA GCACATATGA ACTACAAAGA 
CCTGGCGCAC CTGTCAATCT GCATTACAAT GTGACGACAG AAGGGGAAGT TATATTCAGG 
TGGACCGGCA CACAACCTGA CAACAACACT ATAAACTATG AGATCCAATA CTCATCCAAT 
TCCTTGCTTC AGCAGTGGAA GGTATTGAAG GTCAAAGGTC GTTCATGGGT TGCTCTGAAT 
GAGCTCAGTT CTGACATCAG ATACACGGTT CAAGTGCGCT GCCAAAACCA TCTCGGCTAC 
TGGAGTGAAT GGAGCCAACC TTTCTATTTC AAACTAGATG TCAGTTACAT CCCTGCTGAA 
GTCTTCACAA CGCAGGAGTC AGAGGTGACA GTTTACTCTG TGTTCCACAA CCGCAGCTGG 
AGCGCCAGTA AAGCCGTGTG GTTTCTGAAC GGCAAGATGA AGATACCAGA AAGCCAGTAC 
AGAGTCATCA ATGAGCAGGT CAGCACAGTC ACTCTAAAGA TGGACAAAGC AGGATTTGAC 
ACTCTGATGT GCTGCCTCAC ATTGGGAGAG AAATCTATGT GCTCCATCGC CTATGCCAAA 
ATATACACAG AAGGAAGGTT TAACGCAAAT ATTACCTGCG AAAGCGAGTA TTCATATGTG 
GACACCATGA TCTGTAAGTG GAATCAGAGC AATTGGGCAC AGGCCAGACT CCTTTACAGA 
CAATACAGAT CAAAGTGCAC TGAAGAAGCT GAAGAAGACA CGTCTTTGGT GAAAGAGTGT 
CCATCTAAAG CAGGAGATCA CAGACAGTGC ACCTTAAGCC AAATCAGCAT GATCTTTTGC 
TATAAATTCT GGCTTGAAGT GGAAGGAGGA CGTGGCCAAT CGTTTCCTGT CTACGTTACG 
CCTATTGACT ATGTGAAACC GTCACCTCCC TTTGATCTGG AGGCAATCAC TCTGCCGAGT 
AAAACCCTGA GTGTAAGGTG GAAACGCCCC TCTTTGCCTG TATACGGCAT GCAGTACGAG 
CTGCAATTCA AGGCTTTGGC TGGAATGGCA AATACGCAAT GGAAGGTCAT TGGTCCTCTA 
TTGGAACCAC AGGCAGAGAT TCAGCTTGAG GAGTCCTGTG TTCACTTTAA AGTAGAAGTT 
CGCTGTAAAG ACGTGAACGA CACTGGATAC TGGAGCGACT GGAGCAATAG TCACATTTCC 
ACTGTTTTCA ACTTGAAAGC ACCAGAGATG GGACCAGACT TCTGGCGCAT TTTACAAGAG 
GATCCTACTA GGAATGTGAC AAACGTTACA CTGATCTTTA AGCAGCCCAT TCTAGCAGGA 
GATCCAAATA GCTGCGTGGA GGGTTTAGTG ATTAAACACC AGGCCTCAGG TGGAGTCATG 
TGGTCAAATG AAACAACCTT GGCTCGGTTT CACTCATTCC AGTGGAGGAA GGAAGCGCAT 
ACAGTCACTG TGATGTCTCG CAACGCTTTG GGCATTTCCA CATGGAACAG AAACATCACG 
CTCTTACGTC AAGCCAAACG GCGATGTGTG CGTTCGTTCA GTGCAGTTGC AAATGTGAGC 
TGTGTGCACC TCTCCTGGAG CCTGTTATCT GATCAGCCGG TGCCTCAGTC ATTTGTTATT 
GAATGGTTAG ACCTGAACAA AGACCCCGAA AAGGATGTGT CTCTCACAGA AAGAATACAG 
TGGGTCAGGG TTGAGTCCAG ATCGAGAGAT CTTTCTCTTT GCCGTCGTTT TTATGGCTCA 
GAGGAGTTCA CCTTGTATCC AGTGTTTGCG GATGGTGAAG GGGAACCGGC TCGATACACA 
GCTACAAGGG GTGATCCTGC AGCGTATATA CTGCTACTGA TCATCGCCTT CCTGTCAGTG 
GTGCTGTTTG TCACACTGAT GATGTCACAG AACCAGATGA AAAAACTCAT GTGGAAGGAT 
GTTCCAAATC CCAACAAGTG CTCTTGGGCC AAAGGAATGG ACTTCAGGCA GATTGACACC 
ATGGAGAGCC TGTTCCCTCA TTCAGAGGGT CTCACTGCCT GTCCATTGCT GCTTGTCTCC 
GAGAGCATCT GTGAGGTTGA AATCATCGAG AAACCTCATC CTCTTACCAT TGAAAACGTA 
AAAGACAATG AAGAACTTCC CTCCGGGGAC AAGACAACCA CAGACTCTGG CCTTCAGGGA 
GACTCTTCTG AAGCTCTGGA AGCATCTACT GCTGCTCCCA CTCCAGAAAC ATCAGGCCAG 
TCTTCAGTCA CGTACTCCAC CATCCTCCTC TCCGACCAGC CCAGCCAACT CCAAAAGCAG 
CAGGAGAGTC TCAGCAGCTC CAGTGACGAA GGCAACTTCT CTGCCAACAA CTCGGACATT 
TCCGGCTCCT TCCCTGGAGG ACTTTGGGAC TTGGAGAACA GCAATCCTCG CCATTCCAGC 
TCCTACAACT CTGTGGAGGA GTTTTCGGAA ACATCAGAAC CTGATTATGA AGCTTCGGAA 
AACACAGGTT TAGCTAAAGA CCTCTATTAC CTCGAGATGA CCGGAGAGGA GAAAGAAGAA 
GAAGAAGAAG AAGAAGAAGA AGAAGAAGAA GAAGACGAAC CAGAGGAGGG TCAAAGTAAG 
AATAAAAGAG TGATGGGTGT AAACCCCAGG CCTCTACTGG AAAGCCAAAA TTCCACAGCC 
AGCAATTCCA ATAACATGTC TCACAGCATT CCTCTTTATC TGCCTCAGTT TCGAAGTGAA 
TGTATCAATC CAACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lepr ( XM_021476908.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332583.1 Danio rerio leptin receptor (lepr), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa11917
Clone ID Related Accession (Same CDS sequence)	NM_001309403.1 , XM_021476908.1 , XM_021476907.1
Accession Version	XM_021476908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	leptin receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCTG TCTTCATCAT GCTTGCACTG CTTGTGATTT TTATTGCCGT TTCACAAGGC 
CTTGCAGATT TGAATCCTTC TGATGGAGTC AGCGATGGTG TTTACGAAGA CCTGAAGTGG 
AAAGCTCTCC TGTGTTGTGA TCATCCTGTT CAGACGCTTA ACAGTGGTCT TTCAGAACAC 
CCACCCGAAC AACACTGCCA ACTGCTAAAT GCTACAAAAC AACAGTCTTT TGGAAGCTGT 
TTGGACATCC TGTGCTGGCT TGAAGGTGAA CGGGAAAATT TGATTTGTAA TGCAAAGACC 
CGTAGAGCAG CAGCAGCTTC CACTCTGGTC AGCGTCAGCC CTCATCAATT AGTTGTACAG 
ATGGATGTAC ATTCAGATGA AGCAAATTCC ACTGCTCAGT GTGTTGGAGA AGAGACTGCC 
ATCTGCTCCG TTTCTCTTCA CGGCGGGGAT GCAACAGTCT CATTAGTTAT AATTATATCT 
GAGAATGGGA CCACTGCACA ATCACAGAAG ATGCAAGTCA GCACATATGA ACTACAAAGA 
CCTGGCGCAC CTGTCAATCT GCATTACAAT GTGACGACAG AAGGGGAAGT TATATTCAGG 
TGGACCGGCA CACAACCTGA CAACAACACT ATAAACTATG AGATCCAATA CTCATCCAAT 
TCCTTGCTTC AGCAGTGGAA GGTATTGAAG GTCAAAGGTC GTTCATGGGT TGCTCTGAAT 
GAGCTCAGTT CTGACATCAG ATACACGGTT CAAGTGCGCT GCCAAAACCA TCTCGGCTAC 
TGGAGTGAAT GGAGCCAACC TTTCTATTTC AAACTAGATG TCAGTTACAT CCCTGCTGAA 
GTCTTCACAA CGCAGGAGTC AGAGGTGACA GTTTACTCTG TGTTCCACAA CCGCAGCTGG 
AGCGCCAGTA AAGCCGTGTG GTTTCTGAAC GGCAAGATGA AGATACCAGA AAGCCAGTAC 
AGAGTCATCA ATGAGCAGGT CAGCACAGTC ACTCTAAAGA TGGACAAAGC AGGATTTGAC 
ACTCTGATGT GCTGCCTCAC ATTGGGAGAG AAATCTATGT GCTCCATCGC CTATGCCAAA 
ATATACACAG AAGGAAGGTT TAACGCAAAT ATTACCTGCG AAAGCGAGTA TTCATATGTG 
GACACCATGA TCTGTAAGTG GAATCAGAGC AATTGGGCAC AGGCCAGACT CCTTTACAGA 
CAATACAGAT CAAAGTGCAC TGAAGAAGCT GAAGAAGACA CGTCTTTGGT GAAAGAGTGT 
CCATCTAAAG CAGGAGATCA CAGACAGTGC ACCTTAAGCC AAATCAGCAT GATCTTTTGC 
TATAAATTCT GGCTTGAAGT GGAAGGAGGA CGTGGCCAAT CGTTTCCTGT CTACGTTACG 
CCTATTGACT ATGTGAAACC GTCACCTCCC TTTGATCTGG AGGCAATCAC TCTGCCGAGT 
AAAACCCTGA GTGTAAGGTG GAAACGCCCC TCTTTGCCTG TATACGGCAT GCAGTACGAG 
CTGCAATTCA AGGCTTTGGC TGGAATGGCA AATACGCAAT GGAAGGTCAT TGGTCCTCTA 
TTGGAACCAC AGGCAGAGAT TCAGCTTGAG GAGTCCTGTG TTCACTTTAA AGTAGAAGTT 
CGCTGTAAAG ACGTGAACGA CACTGGATAC TGGAGCGACT GGAGCAATAG TCACATTTCC 
ACTGTTTTCA ACTTGAAAGC ACCAGAGATG GGACCAGACT TCTGGCGCAT TTTACAAGAG 
GATCCTACTA GGAATGTGAC AAACGTTACA CTGATCTTTA AGCAGCCCAT TCTAGCAGGA 
GATCCAAATA GCTGCGTGGA GGGTTTAGTG ATTAAACACC AGGCCTCAGG TGGAGTCATG 
TGGTCAAATG AAACAACCTT GGCTCGGTTT CACTCATTCC AGTGGAGGAA GGAAGCGCAT 
ACAGTCACTG TGATGTCTCG CAACGCTTTG GGCATTTCCA CATGGAACAG AAACATCACG 
CTCTTACGTC AAGCCAAACG GCGATGTGTG CGTTCGTTCA GTGCAGTTGC AAATGTGAGC 
TGTGTGCACC TCTCCTGGAG CCTGTTATCT GATCAGCCGG TGCCTCAGTC ATTTGTTATT 
GAATGGTTAG ACCTGAACAA AGACCCCGAA AAGGATGTGT CTCTCACAGA AAGAATACAG 
TGGGTCAGGG TTGAGTCCAG ATCGAGAGAT CTTTCTCTTT GCCGTCGTTT TTATGGCTCA 
GAGGAGTTCA CCTTGTATCC AGTGTTTGCG GATGGTGAAG GGGAACCGGC TCGATACACA 
GCTACAAGGG GTGATCCTGC AGCGTATATA CTGCTACTGA TCATCGCCTT CCTGTCAGTG 
GTGCTGTTTG TCACACTGAT GATGTCACAG AACCAGATGA AAAAACTCAT GTGGAAGGAT 
GTTCCAAATC CCAACAAGTG CTCTTGGGCC AAAGGAATGG ACTTCAGGCA GATTGACACC 
ATGGAGAGCC TGTTCCCTCA TTCAGAGGGT CTCACTGCCT GTCCATTGCT GCTTGTCTCC 
GAGAGCATCT GTGAGGTTGA AATCATCGAG AAACCTCATC CTCTTACCAT TGAAAACGTA 
AAAGACAATG AAGAACTTCC CTCCGGGGAC AAGACAACCA CAGACTCTGG CCTTCAGGGA 
GACTCTTCTG AAGCTCTGGA AGCATCTACT GCTGCTCCCA CTCCAGAAAC ATCAGGCCAG 
TCTTCAGTCA CGTACTCCAC CATCCTCCTC TCCGACCAGC CCAGCCAACT CCAAAAGCAG 
CAGGAGAGTC TCAGCAGCTC CAGTGACGAA GGCAACTTCT CTGCCAACAA CTCGGACATT 
TCCGGCTCCT TCCCTGGAGG ACTTTGGGAC TTGGAGAACA GCAATCCTCG CCATTCCAGC 
TCCTACAACT CTGTGGAGGA GTTTTCGGAA ACATCAGAAC CTGATTATGA AGCTTCGGAA 
AACACAGGTT TAGCTAAAGA CCTCTATTAC CTCGAGATGA CCGGAGAGGA GAAAGAAGAA 
GAAGAAGAAG AAGAAGAAGA AGAAGAAGAA GAAGACGAAC CAGAGGAGGG TCAAAGTAAG 
AATAAAAGAG TGATGGGTGT AAACCCCAGG CCTCTACTGG AAAGCCAAAA TTCCACAGCC 
AGCAATTCCA ATAACATGTC TCACAGCATT CCTCTTTATC TGCCTCAGTT TCGAAGTGAA 
TGTATCAATC CAACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332583.1
CDS	326..3523
Translation	MMSVFIMLALLVIFIAVSQGLADLNPSDGVSDGVYEDLKWKALLCCDHPVQTLNSGLSEHPPEQHCQLLNATKQQSFGSCLDILCWLEGERENLICNAKTRRAAAASTLVSVSPHQLVVQMDVHSDEANSTAQCVGEETAICSVSLHGGDATVSLVIIISENGTTAQSQKMQVSTYELQRPGAPVNLHYNVTTEGEVIFRWTGTQPDNNTINYEIQYSSNSLLQQWKVLKVKGRSWVALNELSSDIRYTVQVRCQNHLGYWSEWSQPFYFKLDVSYIPAEVFTTQESEVTVYSVFHNRSWSASKAVWFLNGKMKIPESQYRVINEQVSTVTLKMDKAGFDTLMCCLTLGEKSMCSIAYAKIYTEGRFNANITCESEYSYVDTMICKWNQSNWAQARLLYRQYRSKCTEEAEEDTSLVKECPSKAGDHRQCTLSQISMIFCYKFWLEVEGGRGQSFPVYVTPIDYVKPSPPFDLEAITLPSKTLSVRWKRPSLPVYGMQYELQFKALAGMANTQWKVIGPLLEPQAEIQLEESCVHFKVEVRCKDVNDTGYWSDWSNSHISTVFNLKAPEMGPDFWRILQEDPTRNVTNVTLIFKQPILAGDPNSCVEGLVIKHQASGGVMWSNETTLARFHSFQWRKEAHTVTVMSRNALGISTWNRNITLLRQAKRRCVRSFSAVANVSCVHLSWSLLSDQPVPQSFVIEWLDLNKDPEKDVSLTERIQWVRVESRSRDLSLCRRFYGSEEFTLYPVFADGEGEPARYTATRGDPAAYILLLIIAFLSVVLFVTLMMSQNQMKKLMWKDVPNPNKCSWAKGMDFRQIDTMESLFPHSEGLTACPLLLVSESICEVEIIEKPHPLTIENVKDNEELPSGDKTTTDSGLQGDSSEALEASTAAPTPETSGQSSVTYSTILLSDQPSQLQKQQESLSSSSDEGNFSANNSDISGSFPGGLWDLENSNPRHSSSYNSVEEFSETSEPDYEASENTGLAKDLYYLEMTGEEKEEEEEEEEEEEEEDEPEEGQSKNKRVMGVNPRPLLESQNSTASNSNNMSHSIPLYLPQFRSECINPT

Target ORF information:

RefSeq Version	XM_021476908.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leptin receptor (lepr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476908.1

ORF Insert Sequence:

ATGATGTCTG TCTTCATCAT GCTTGCACTG CTTGTGATTT TTATTGCCGT TTCACAAGGC 
CTTGCAGATT TGAATCCTTC TGATGGAGTC AGCGATGGTG TTTACGAAGA CCTGAAGTGG 
AAAGCTCTCC TGTGTTGTGA TCATCCTGTT CAGACGCTTA ACAGTGGTCT TTCAGAACAC 
CCACCCGAAC AACACTGCCA ACTGCTAAAT GCTACAAAAC AACAGTCTTT TGGAAGCTGT 
TTGGACATCC TGTGCTGGCT TGAAGGTGAA CGGGAAAATT TGATTTGTAA TGCAAAGACC 
CGTAGAGCAG CAGCAGCTTC CACTCTGGTC AGCGTCAGCC CTCATCAATT AGTTGTACAG 
ATGGATGTAC ATTCAGATGA AGCAAATTCC ACTGCTCAGT GTGTTGGAGA AGAGACTGCC 
ATCTGCTCCG TTTCTCTTCA CGGCGGGGAT GCAACAGTCT CATTAGTTAT AATTATATCT 
GAGAATGGGA CCACTGCACA ATCACAGAAG ATGCAAGTCA GCACATATGA ACTACAAAGA 
CCTGGCGCAC CTGTCAATCT GCATTACAAT GTGACGACAG AAGGGGAAGT TATATTCAGG 
TGGACCGGCA CACAACCTGA CAACAACACT ATAAACTATG AGATCCAATA CTCATCCAAT 
TCCTTGCTTC AGCAGTGGAA GGTATTGAAG GTCAAAGGTC GTTCATGGGT TGCTCTGAAT 
GAGCTCAGTT CTGACATCAG ATACACGGTT CAAGTGCGCT GCCAAAACCA TCTCGGCTAC 
TGGAGTGAAT GGAGCCAACC TTTCTATTTC AAACTAGATG TCAGTTACAT CCCTGCTGAA 
GTCTTCACAA CGCAGGAGTC AGAGGTGACA GTTTACTCTG TGTTCCACAA CCGCAGCTGG 
AGCGCCAGTA AAGCCGTGTG GTTTCTGAAC GGCAAGATGA AGATACCAGA AAGCCAGTAC 
AGAGTCATCA ATGAGCAGGT CAGCACAGTC ACTCTAAAGA TGGACAAAGC AGGATTTGAC 
ACTCTGATGT GCTGCCTCAC ATTGGGAGAG AAATCTATGT GCTCCATCGC CTATGCCAAA 
ATATACACAG AAGGAAGGTT TAACGCAAAT ATTACCTGCG AAAGCGAGTA TTCATATGTG 
GACACCATGA TCTGTAAGTG GAATCAGAGC AATTGGGCAC AGGCCAGACT CCTTTACAGA 
CAATACAGAT CAAAGTGCAC TGAAGAAGCT GAAGAAGACA CGTCTTTGGT GAAAGAGTGT 
CCATCTAAAG CAGGAGATCA CAGACAGTGC ACCTTAAGCC AAATCAGCAT GATCTTTTGC 
TATAAATTCT GGCTTGAAGT GGAAGGAGGA CGTGGCCAAT CGTTTCCTGT CTACGTTACG 
CCTATTGACT ATGTGAAACC GTCACCTCCC TTTGATCTGG AGGCAATCAC TCTGCCGAGT 
AAAACCCTGA GTGTAAGGTG GAAACGCCCC TCTTTGCCTG TATACGGCAT GCAGTACGAG 
CTGCAATTCA AGGCTTTGGC TGGAATGGCA AATACGCAAT GGAAGGTCAT TGGTCCTCTA 
TTGGAACCAC AGGCAGAGAT TCAGCTTGAG GAGTCCTGTG TTCACTTTAA AGTAGAAGTT 
CGCTGTAAAG ACGTGAACGA CACTGGATAC TGGAGCGACT GGAGCAATAG TCACATTTCC 
ACTGTTTTCA ACTTGAAAGC ACCAGAGATG GGACCAGACT TCTGGCGCAT TTTACAAGAG 
GATCCTACTA GGAATGTGAC AAACGTTACA CTGATCTTTA AGCAGCCCAT TCTAGCAGGA 
GATCCAAATA GCTGCGTGGA GGGTTTAGTG ATTAAACACC AGGCCTCAGG TGGAGTCATG 
TGGTCAAATG AAACAACCTT GGCTCGGTTT CACTCATTCC AGTGGAGGAA GGAAGCGCAT 
ACAGTCACTG TGATGTCTCG CAACGCTTTG GGCATTTCCA CATGGAACAG AAACATCACG 
CTCTTACGTC AAGCCAAACG GCGATGTGTG CGTTCGTTCA GTGCAGTTGC AAATGTGAGC 
TGTGTGCACC TCTCCTGGAG CCTGTTATCT GATCAGCCGG TGCCTCAGTC ATTTGTTATT 
GAATGGTTAG ACCTGAACAA AGACCCCGAA AAGGATGTGT CTCTCACAGA AAGAATACAG 
TGGGTCAGGG TTGAGTCCAG ATCGAGAGAT CTTTCTCTTT GCCGTCGTTT TTATGGCTCA 
GAGGAGTTCA CCTTGTATCC AGTGTTTGCG GATGGTGAAG GGGAACCGGC TCGATACACA 
GCTACAAGGG GTGATCCTGC AGCGTATATA CTGCTACTGA TCATCGCCTT CCTGTCAGTG 
GTGCTGTTTG TCACACTGAT GATGTCACAG AACCAGATGA AAAAACTCAT GTGGAAGGAT 
GTTCCAAATC CCAACAAGTG CTCTTGGGCC AAAGGAATGG ACTTCAGGCA GATTGACACC 
ATGGAGAGCC TGTTCCCTCA TTCAGAGGGT CTCACTGCCT GTCCATTGCT GCTTGTCTCC 
GAGAGCATCT GTGAGGTTGA AATCATCGAG AAACCTCATC CTCTTACCAT TGAAAACGTA 
AAAGACAATG AAGAACTTCC CTCCGGGGAC AAGACAACCA CAGACTCTGG CCTTCAGGGA 
GACTCTTCTG AAGCTCTGGA AGCATCTACT GCTGCTCCCA CTCCAGAAAC ATCAGGCCAG 
TCTTCAGTCA CGTACTCCAC CATCCTCCTC TCCGACCAGC CCAGCCAACT CCAAAAGCAG 
CAGGAGAGTC TCAGCAGCTC CAGTGACGAA GGCAACTTCT CTGCCAACAA CTCGGACATT 
TCCGGCTCCT TCCCTGGAGG ACTTTGGGAC TTGGAGAACA GCAATCCTCG CCATTCCAGC 
TCCTACAACT CTGTGGAGGA GTTTTCGGAA ACATCAGAAC CTGATTATGA AGCTTCGGAA 
AACACAGGTT TAGCTAAAGA CCTCTATTAC CTCGAGATGA CCGGAGAGGA GAAAGAAGAA 
GAAGAAGAAG AAGAAGAAGA AGAAGAAGAA GAAGACGAAC CAGAGGAGGG TCAAAGTAAG 
AATAAAAGAG TGATGGGTGT AAACCCCAGG CCTCTACTGG AAAGCCAAAA TTCCACAGCC 
AGCAATTCCA ATAACATGTC TCACAGCATT CCTCTTTATC TGCCTCAGTT TCGAAGTGAA 
TGTATCAATC CAACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lepr ( XM_021476907.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332582.1 Danio rerio leptin receptor (lepr), transcript variant X1, mRNA.

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CloneID	ODa11917
Clone ID Related Accession (Same CDS sequence)	NM_001309403.1 , XM_021476908.1 , XM_021476907.1
Accession Version	XM_021476907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	leptin receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCTG TCTTCATCAT GCTTGCACTG CTTGTGATTT TTATTGCCGT TTCACAAGGC 
CTTGCAGATT TGAATCCTTC TGATGGAGTC AGCGATGGTG TTTACGAAGA CCTGAAGTGG 
AAAGCTCTCC TGTGTTGTGA TCATCCTGTT CAGACGCTTA ACAGTGGTCT TTCAGAACAC 
CCACCCGAAC AACACTGCCA ACTGCTAAAT GCTACAAAAC AACAGTCTTT TGGAAGCTGT 
TTGGACATCC TGTGCTGGCT TGAAGGTGAA CGGGAAAATT TGATTTGTAA TGCAAAGACC 
CGTAGAGCAG CAGCAGCTTC CACTCTGGTC AGCGTCAGCC CTCATCAATT AGTTGTACAG 
ATGGATGTAC ATTCAGATGA AGCAAATTCC ACTGCTCAGT GTGTTGGAGA AGAGACTGCC 
ATCTGCTCCG TTTCTCTTCA CGGCGGGGAT GCAACAGTCT CATTAGTTAT AATTATATCT 
GAGAATGGGA CCACTGCACA ATCACAGAAG ATGCAAGTCA GCACATATGA ACTACAAAGA 
CCTGGCGCAC CTGTCAATCT GCATTACAAT GTGACGACAG AAGGGGAAGT TATATTCAGG 
TGGACCGGCA CACAACCTGA CAACAACACT ATAAACTATG AGATCCAATA CTCATCCAAT 
TCCTTGCTTC AGCAGTGGAA GGTATTGAAG GTCAAAGGTC GTTCATGGGT TGCTCTGAAT 
GAGCTCAGTT CTGACATCAG ATACACGGTT CAAGTGCGCT GCCAAAACCA TCTCGGCTAC 
TGGAGTGAAT GGAGCCAACC TTTCTATTTC AAACTAGATG TCAGTTACAT CCCTGCTGAA 
GTCTTCACAA CGCAGGAGTC AGAGGTGACA GTTTACTCTG TGTTCCACAA CCGCAGCTGG 
AGCGCCAGTA AAGCCGTGTG GTTTCTGAAC GGCAAGATGA AGATACCAGA AAGCCAGTAC 
AGAGTCATCA ATGAGCAGGT CAGCACAGTC ACTCTAAAGA TGGACAAAGC AGGATTTGAC 
ACTCTGATGT GCTGCCTCAC ATTGGGAGAG AAATCTATGT GCTCCATCGC CTATGCCAAA 
ATATACACAG AAGGAAGGTT TAACGCAAAT ATTACCTGCG AAAGCGAGTA TTCATATGTG 
GACACCATGA TCTGTAAGTG GAATCAGAGC AATTGGGCAC AGGCCAGACT CCTTTACAGA 
CAATACAGAT CAAAGTGCAC TGAAGAAGCT GAAGAAGACA CGTCTTTGGT GAAAGAGTGT 
CCATCTAAAG CAGGAGATCA CAGACAGTGC ACCTTAAGCC AAATCAGCAT GATCTTTTGC 
TATAAATTCT GGCTTGAAGT GGAAGGAGGA CGTGGCCAAT CGTTTCCTGT CTACGTTACG 
CCTATTGACT ATGTGAAACC GTCACCTCCC TTTGATCTGG AGGCAATCAC TCTGCCGAGT 
AAAACCCTGA GTGTAAGGTG GAAACGCCCC TCTTTGCCTG TATACGGCAT GCAGTACGAG 
CTGCAATTCA AGGCTTTGGC TGGAATGGCA AATACGCAAT GGAAGGTCAT TGGTCCTCTA 
TTGGAACCAC AGGCAGAGAT TCAGCTTGAG GAGTCCTGTG TTCACTTTAA AGTAGAAGTT 
CGCTGTAAAG ACGTGAACGA CACTGGATAC TGGAGCGACT GGAGCAATAG TCACATTTCC 
ACTGTTTTCA ACTTGAAAGC ACCAGAGATG GGACCAGACT TCTGGCGCAT TTTACAAGAG 
GATCCTACTA GGAATGTGAC AAACGTTACA CTGATCTTTA AGCAGCCCAT TCTAGCAGGA 
GATCCAAATA GCTGCGTGGA GGGTTTAGTG ATTAAACACC AGGCCTCAGG TGGAGTCATG 
TGGTCAAATG AAACAACCTT GGCTCGGTTT CACTCATTCC AGTGGAGGAA GGAAGCGCAT 
ACAGTCACTG TGATGTCTCG CAACGCTTTG GGCATTTCCA CATGGAACAG AAACATCACG 
CTCTTACGTC AAGCCAAACG GCGATGTGTG CGTTCGTTCA GTGCAGTTGC AAATGTGAGC 
TGTGTGCACC TCTCCTGGAG CCTGTTATCT GATCAGCCGG TGCCTCAGTC ATTTGTTATT 
GAATGGTTAG ACCTGAACAA AGACCCCGAA AAGGATGTGT CTCTCACAGA AAGAATACAG 
TGGGTCAGGG TTGAGTCCAG ATCGAGAGAT CTTTCTCTTT GCCGTCGTTT TTATGGCTCA 
GAGGAGTTCA CCTTGTATCC AGTGTTTGCG GATGGTGAAG GGGAACCGGC TCGATACACA 
GCTACAAGGG GTGATCCTGC AGCGTATATA CTGCTACTGA TCATCGCCTT CCTGTCAGTG 
GTGCTGTTTG TCACACTGAT GATGTCACAG AACCAGATGA AAAAACTCAT GTGGAAGGAT 
GTTCCAAATC CCAACAAGTG CTCTTGGGCC AAAGGAATGG ACTTCAGGCA GATTGACACC 
ATGGAGAGCC TGTTCCCTCA TTCAGAGGGT CTCACTGCCT GTCCATTGCT GCTTGTCTCC 
GAGAGCATCT GTGAGGTTGA AATCATCGAG AAACCTCATC CTCTTACCAT TGAAAACGTA 
AAAGACAATG AAGAACTTCC CTCCGGGGAC AAGACAACCA CAGACTCTGG CCTTCAGGGA 
GACTCTTCTG AAGCTCTGGA AGCATCTACT GCTGCTCCCA CTCCAGAAAC ATCAGGCCAG 
TCTTCAGTCA CGTACTCCAC CATCCTCCTC TCCGACCAGC CCAGCCAACT CCAAAAGCAG 
CAGGAGAGTC TCAGCAGCTC CAGTGACGAA GGCAACTTCT CTGCCAACAA CTCGGACATT 
TCCGGCTCCT TCCCTGGAGG ACTTTGGGAC TTGGAGAACA GCAATCCTCG CCATTCCAGC 
TCCTACAACT CTGTGGAGGA GTTTTCGGAA ACATCAGAAC CTGATTATGA AGCTTCGGAA 
AACACAGGTT TAGCTAAAGA CCTCTATTAC CTCGAGATGA CCGGAGAGGA GAAAGAAGAA 
GAAGAAGAAG AAGAAGAAGA AGAAGAAGAA GAAGACGAAC CAGAGGAGGG TCAAAGTAAG 
AATAAAAGAG TGATGGGTGT AAACCCCAGG CCTCTACTGG AAAGCCAAAA TTCCACAGCC 
AGCAATTCCA ATAACATGTC TCACAGCATT CCTCTTTATC TGCCTCAGTT TCGAAGTGAA 
TGTATCAATC CAACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332582.1
CDS	94..3291
Translation	MMSVFIMLALLVIFIAVSQGLADLNPSDGVSDGVYEDLKWKALLCCDHPVQTLNSGLSEHPPEQHCQLLNATKQQSFGSCLDILCWLEGERENLICNAKTRRAAAASTLVSVSPHQLVVQMDVHSDEANSTAQCVGEETAICSVSLHGGDATVSLVIIISENGTTAQSQKMQVSTYELQRPGAPVNLHYNVTTEGEVIFRWTGTQPDNNTINYEIQYSSNSLLQQWKVLKVKGRSWVALNELSSDIRYTVQVRCQNHLGYWSEWSQPFYFKLDVSYIPAEVFTTQESEVTVYSVFHNRSWSASKAVWFLNGKMKIPESQYRVINEQVSTVTLKMDKAGFDTLMCCLTLGEKSMCSIAYAKIYTEGRFNANITCESEYSYVDTMICKWNQSNWAQARLLYRQYRSKCTEEAEEDTSLVKECPSKAGDHRQCTLSQISMIFCYKFWLEVEGGRGQSFPVYVTPIDYVKPSPPFDLEAITLPSKTLSVRWKRPSLPVYGMQYELQFKALAGMANTQWKVIGPLLEPQAEIQLEESCVHFKVEVRCKDVNDTGYWSDWSNSHISTVFNLKAPEMGPDFWRILQEDPTRNVTNVTLIFKQPILAGDPNSCVEGLVIKHQASGGVMWSNETTLARFHSFQWRKEAHTVTVMSRNALGISTWNRNITLLRQAKRRCVRSFSAVANVSCVHLSWSLLSDQPVPQSFVIEWLDLNKDPEKDVSLTERIQWVRVESRSRDLSLCRRFYGSEEFTLYPVFADGEGEPARYTATRGDPAAYILLLIIAFLSVVLFVTLMMSQNQMKKLMWKDVPNPNKCSWAKGMDFRQIDTMESLFPHSEGLTACPLLLVSESICEVEIIEKPHPLTIENVKDNEELPSGDKTTTDSGLQGDSSEALEASTAAPTPETSGQSSVTYSTILLSDQPSQLQKQQESLSSSSDEGNFSANNSDISGSFPGGLWDLENSNPRHSSSYNSVEEFSETSEPDYEASENTGLAKDLYYLEMTGEEKEEEEEEEEEEEEEDEPEEGQSKNKRVMGVNPRPLLESQNSTASNSNNMSHSIPLYLPQFRSECINPT

Target ORF information:

RefSeq Version	XM_021476907.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leptin receptor (lepr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476907.1

ORF Insert Sequence:

ATGATGTCTG TCTTCATCAT GCTTGCACTG CTTGTGATTT TTATTGCCGT TTCACAAGGC 
CTTGCAGATT TGAATCCTTC TGATGGAGTC AGCGATGGTG TTTACGAAGA CCTGAAGTGG 
AAAGCTCTCC TGTGTTGTGA TCATCCTGTT CAGACGCTTA ACAGTGGTCT TTCAGAACAC 
CCACCCGAAC AACACTGCCA ACTGCTAAAT GCTACAAAAC AACAGTCTTT TGGAAGCTGT 
TTGGACATCC TGTGCTGGCT TGAAGGTGAA CGGGAAAATT TGATTTGTAA TGCAAAGACC 
CGTAGAGCAG CAGCAGCTTC CACTCTGGTC AGCGTCAGCC CTCATCAATT AGTTGTACAG 
ATGGATGTAC ATTCAGATGA AGCAAATTCC ACTGCTCAGT GTGTTGGAGA AGAGACTGCC 
ATCTGCTCCG TTTCTCTTCA CGGCGGGGAT GCAACAGTCT CATTAGTTAT AATTATATCT 
GAGAATGGGA CCACTGCACA ATCACAGAAG ATGCAAGTCA GCACATATGA ACTACAAAGA 
CCTGGCGCAC CTGTCAATCT GCATTACAAT GTGACGACAG AAGGGGAAGT TATATTCAGG 
TGGACCGGCA CACAACCTGA CAACAACACT ATAAACTATG AGATCCAATA CTCATCCAAT 
TCCTTGCTTC AGCAGTGGAA GGTATTGAAG GTCAAAGGTC GTTCATGGGT TGCTCTGAAT 
GAGCTCAGTT CTGACATCAG ATACACGGTT CAAGTGCGCT GCCAAAACCA TCTCGGCTAC 
TGGAGTGAAT GGAGCCAACC TTTCTATTTC AAACTAGATG TCAGTTACAT CCCTGCTGAA 
GTCTTCACAA CGCAGGAGTC AGAGGTGACA GTTTACTCTG TGTTCCACAA CCGCAGCTGG 
AGCGCCAGTA AAGCCGTGTG GTTTCTGAAC GGCAAGATGA AGATACCAGA AAGCCAGTAC 
AGAGTCATCA ATGAGCAGGT CAGCACAGTC ACTCTAAAGA TGGACAAAGC AGGATTTGAC 
ACTCTGATGT GCTGCCTCAC ATTGGGAGAG AAATCTATGT GCTCCATCGC CTATGCCAAA 
ATATACACAG AAGGAAGGTT TAACGCAAAT ATTACCTGCG AAAGCGAGTA TTCATATGTG 
GACACCATGA TCTGTAAGTG GAATCAGAGC AATTGGGCAC AGGCCAGACT CCTTTACAGA 
CAATACAGAT CAAAGTGCAC TGAAGAAGCT GAAGAAGACA CGTCTTTGGT GAAAGAGTGT 
CCATCTAAAG CAGGAGATCA CAGACAGTGC ACCTTAAGCC AAATCAGCAT GATCTTTTGC 
TATAAATTCT GGCTTGAAGT GGAAGGAGGA CGTGGCCAAT CGTTTCCTGT CTACGTTACG 
CCTATTGACT ATGTGAAACC GTCACCTCCC TTTGATCTGG AGGCAATCAC TCTGCCGAGT 
AAAACCCTGA GTGTAAGGTG GAAACGCCCC TCTTTGCCTG TATACGGCAT GCAGTACGAG 
CTGCAATTCA AGGCTTTGGC TGGAATGGCA AATACGCAAT GGAAGGTCAT TGGTCCTCTA 
TTGGAACCAC AGGCAGAGAT TCAGCTTGAG GAGTCCTGTG TTCACTTTAA AGTAGAAGTT 
CGCTGTAAAG ACGTGAACGA CACTGGATAC TGGAGCGACT GGAGCAATAG TCACATTTCC 
ACTGTTTTCA ACTTGAAAGC ACCAGAGATG GGACCAGACT TCTGGCGCAT TTTACAAGAG 
GATCCTACTA GGAATGTGAC AAACGTTACA CTGATCTTTA AGCAGCCCAT TCTAGCAGGA 
GATCCAAATA GCTGCGTGGA GGGTTTAGTG ATTAAACACC AGGCCTCAGG TGGAGTCATG 
TGGTCAAATG AAACAACCTT GGCTCGGTTT CACTCATTCC AGTGGAGGAA GGAAGCGCAT 
ACAGTCACTG TGATGTCTCG CAACGCTTTG GGCATTTCCA CATGGAACAG AAACATCACG 
CTCTTACGTC AAGCCAAACG GCGATGTGTG CGTTCGTTCA GTGCAGTTGC AAATGTGAGC 
TGTGTGCACC TCTCCTGGAG CCTGTTATCT GATCAGCCGG TGCCTCAGTC ATTTGTTATT 
GAATGGTTAG ACCTGAACAA AGACCCCGAA AAGGATGTGT CTCTCACAGA AAGAATACAG 
TGGGTCAGGG TTGAGTCCAG ATCGAGAGAT CTTTCTCTTT GCCGTCGTTT TTATGGCTCA 
GAGGAGTTCA CCTTGTATCC AGTGTTTGCG GATGGTGAAG GGGAACCGGC TCGATACACA 
GCTACAAGGG GTGATCCTGC AGCGTATATA CTGCTACTGA TCATCGCCTT CCTGTCAGTG 
GTGCTGTTTG TCACACTGAT GATGTCACAG AACCAGATGA AAAAACTCAT GTGGAAGGAT 
GTTCCAAATC CCAACAAGTG CTCTTGGGCC AAAGGAATGG ACTTCAGGCA GATTGACACC 
ATGGAGAGCC TGTTCCCTCA TTCAGAGGGT CTCACTGCCT GTCCATTGCT GCTTGTCTCC 
GAGAGCATCT GTGAGGTTGA AATCATCGAG AAACCTCATC CTCTTACCAT TGAAAACGTA 
AAAGACAATG AAGAACTTCC CTCCGGGGAC AAGACAACCA CAGACTCTGG CCTTCAGGGA 
GACTCTTCTG AAGCTCTGGA AGCATCTACT GCTGCTCCCA CTCCAGAAAC ATCAGGCCAG 
TCTTCAGTCA CGTACTCCAC CATCCTCCTC TCCGACCAGC CCAGCCAACT CCAAAAGCAG 
CAGGAGAGTC TCAGCAGCTC CAGTGACGAA GGCAACTTCT CTGCCAACAA CTCGGACATT 
TCCGGCTCCT TCCCTGGAGG ACTTTGGGAC TTGGAGAACA GCAATCCTCG CCATTCCAGC 
TCCTACAACT CTGTGGAGGA GTTTTCGGAA ACATCAGAAC CTGATTATGA AGCTTCGGAA 
AACACAGGTT TAGCTAAAGA CCTCTATTAC CTCGAGATGA CCGGAGAGGA GAAAGAAGAA 
GAAGAAGAAG AAGAAGAAGA AGAAGAAGAA GAAGACGAAC CAGAGGAGGG TCAAAGTAAG 
AATAAAAGAG TGATGGGTGT AAACCCCAGG CCTCTACTGG AAAGCCAAAA TTCCACAGCC 
AGCAATTCCA ATAACATGTC TCACAGCATT CCTCTTTATC TGCCTCAGTT TCGAAGTGAA 
TGTATCAATC CAACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Examining the responses of the zebrafish (Danio rerio) gastrointestinal system to the suspected obesogen diethylhexyl phthalate.
Environmental pollution (Barking, Essex : 1987)2451086-1094(2019 Feb)
Buerger AN,Schmidt J,Chase A,Paixao C,Patel TN,Brumback BA,Kane AS,Martyniuk CJ,Bisesi JH Jr

Leptin induces muscle wasting in a zebrafish
Disease models & mechanisms12(2)(2019 02)
Yang Q,Yan C,Wang X,Gong Z

Neuroinflammatory signals drive spinal curve formation in zebrafish models of idiopathic scoliosis.
Science advances4(12)eaav1781(2018 12)
Van Gennip JLM,Boswell CW,Ciruna B

Histone Deacetylase-Mediated Müller Glia Reprogramming through Her4.1-Lin28a Axis Is Essential for Retina Regeneration in Zebrafish.
iScience768-84(2018 Sep)
Mitra S,Sharma P,Kaur S,Khursheed MA,Gupta S,Ahuja R,Kurup AJ,Chaudhary M,Ramachandran R

Frontiers in physiology9676(2018)
Huang P,Xiong S,Kang J,Mei J,Gui JF

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lepr cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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