dpagt1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dpagt1
Entrez Gene ID	566539
Full Name	dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)
Synonyms	si:ch211-117m20.1
General protein information	Preferred Names dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) Names UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001089411.1	NP_001082880.1	UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	dpagt1	NP_001082880.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008131	XP_317328.3
Magnaporthe oryzae (rice blast fungus)	MGG_01559	XP_003714534.1
Pan troglodytes (chimpanzee)	DPAGT1	XP_001165129.1
Gallus gallus (chicken)	DPAGT1	XP_417845.2
Schizosaccharomyces pombe (fission yeast)	gpt2	NP_596244.1
Canis lupus familiaris (dog)	DPAGT1	XP_546489.3
Mus musculus (house mouse)	Dpagt1	NP_031901.2
Arabidopsis thaliana (thale cress)	AT3G57220	NP_191281.1
Xenopus tropicalis (tropical clawed frog)	LOC100487779	XP_002935051.2
Macaca mulatta (Rhesus monkey)	DPAGT1	XP_001102857.1
Rattus norvegicus (Norway rat)	Dpagt1	NP_955420.1
Eremothecium gossypii	AGOS_AFL037W	NP_985511.1
Homo sapiens (human)	DPAGT1	NP_001373.2
Bos taurus (cattle)	DPAGT1	NP_001015664.1
Drosophila melanogaster (fruit fly)	CG5287	NP_609608.1
Caenorhabditis elegans (roundworm)	CELE_Y60A3A.14	NP_507859.2
Saccharomyces cerevisiae (baker's yeast)	ALG7	NP_009802.3
Arabidopsis thaliana (thale cress)	GPT	NP_565950.1
Kluyveromyces lactis	KLLA0F06776g	XP_455387.1
Neurospora crassa	NCU10762	XP_001728541.1
Oryza sativa (rice)	Os07g0661100	NP_001060535.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

trappc11 is required for protein glycosylation in zebrafish and humans.
DeRossi C, Vacaru A, Rafiq R, Cinaroglu A, Imrie D, Nayar S, Baryshnikova A, Milev MP, Stanga D, Kadakia D, Gao N, Chu J, Freeze HH, Lehrman MA, Sacher M, Sadler KC
Molecular biology of the cell27(8)1220-34(2016 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Characterization of a BAC library from channel catfish Ictalurus punctatus: indications of high levels of chromosomal reshuffling among teleost genomes.
Wang S, Xu P, Thorsen J, Zhu B, de Jong PJ, Waldbieser G, Kucuktas H, Liu Z
Marine biotechnology (New York, N.Y.)9(6)701-11(2007 Nov-Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dpagt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dpagt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31456	NM_001089411.1 Latest version!	Danio rerio dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (dpagt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dpagt1 ( NM_001089411.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001082880.1 Danio rerio dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (dpagt1), mRNA.

Price & Availability↑

CloneID	ODa31456
Clone ID Related Accession (Same CDS sequence)	NM_001089411.1
Accession Version	NM_001089411.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC139558.1. On May 24, 2007 this sequence version replaced XM_689814.2. ##Evidence-Data-START## Transcript exon combination :: BC139558.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCCAA TTCCTGTTGT TCCTCTGATC ATCAACTGTT GTATGTCTGC ACTTGGGTGC 
ATTGCAACAG TCAAGCTTAT TCCTGCCTTT AAGGAACATT TCATATCTGC AAGACTTTAT 
GGCATGGACT TAAATAAAAC CACAAAGAAA GAGGTACCCG AGTCACAGGG TGTAATCAGT 
GGGACAGTCT TCCTCATCAT CCTCTTCCTC TTCATCCCTG TTCCATTCTT ACAGTGCTTT 
ATGGGAGAGA AGTGTCAACG GTTTCCTCAC AACGAGTTTG TTCAGCTGAT CGGTGCATTG 
TTGGCCATCT GCTGCATGAT TTTCCTGGGC TTTGCTGATG ATGTTCTGAA CCTGCGATGG 
AGACACAAAC TTCTGCTGCC CACCATGGCC TCTCTGCCTC TCCTCATGGT TTACTTCACT 
AACTTTGGCA ACACTGTCAT CGTCGTGCCA AAACCTTTCC GTCTCCTTTT GGGGATGCAT 
TTGGACCTGG GAATTCTGTA TTATGTGTAT ATGGGAATGT TGGCTGTGTT TTGCACAAAT 
GCGATCAACA TCTTGGCTGG CATCAATGGC ATTGAGTCTG GACAGGCTCT CTTCATATCA 
GGGTCCATCA TCCTCTTTAA TTTACTGGAA CTCAATGGAG ACTACAGAGA TGACCATGTT 
TTCTCCTTGT ACTTCATGAT TCCTTTTTTC TTCACCACAT TAGCTCTCTT CTACCACAAT 
TGGTATCCCT CTTCTGTGTT TGTGGGAGAC ACTTTCTGTT ACTTTGCTGG AATGACATTT 
GCAGTTGTCG GGATACTCGG CCACTTTAGC AAGACAATGC TGCTTTTCTT CATCCCTCAA 
GTCATCAACT TTATTTACTC ACTGCCTCAG CTCTTTCACA TCATTCCCTG CCCTAGACAT 
CGCCTGCCTA GGTTACAGTC AGACACAGGA AAGCTGGGCA TGAGCTACTC CAAATTCAAA 
CAGAAAGACC TTGGCAAATT AGGGCAACTT ATTCTAAAGG TGGCAGAAAA GTTATGGGTT 
CTGGATGTGC GTAGAGGGCA GGAAGGAGAT GATGAGTTTA TTGAGTGTAA CAATATGACC 
CTTATCAATC TGGTGCTGAA AATACTGGGA CCTACCCATG AGAGAACATT GACTGCAATC 
ATGCTGTTAA TGCAGGTTTT GGGGAGTGCT GTGGCATTTG GCATTCGTTA TCACCTTGTG 
CGTCTGTTCT ATGACGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001082880.1
CDS	332..1552
Translation	MSPIPVVPLIINCCMSALGCIATVKLIPAFKEHFISARLYGMDLNKTTKKEVPESQGVISGTVFLIILFLFIPVPFLQCFMGEKCQRFPHNEFVQLIGALLAICCMIFLGFADDVLNLRWRHKLLLPTMASLPLLMVYFTNFGNTVIVVPKPFRLLLGMHLDLGILYYVYMGMLAVFCTNAINILAGINGIESGQALFISGSIILFNLLELNGDYRDDHVFSLYFMIPFFFTTLALFYHNWYPSSVFVGDTFCYFAGMTFAVVGILGHFSKTMLLFFIPQVINFIYSLPQLFHIIPCPRHRLPRLQSDTGKLGMSYSKFKQKDLGKLGQLILKVAEKLWVLDVRRGQEGDDEFIECNNMTLINLVLKILGPTHERTLTAIMLLMQVLGSAVAFGIRYHLVRLFYDV

Target ORF information:

RefSeq Version	NM_001089411.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (dpagt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001089411.1

ORF Insert Sequence:

ATGTCTCCAA TTCCTGTTGT TCCTCTGATC ATCAACTGTT GTATGTCTGC ACTTGGGTGC 
ATTGCAACAG TCAAGCTTAT TCCTGCCTTT AAGGAACATT TCATATCTGC AAGACTTTAT 
GGCATGGACT TAAATAAAAC CACAAAGAAA GAGGTACCCG AGTCACAGGG TGTAATCAGT 
GGGACAGTCT TCCTCATCAT CCTCTTCCTC TTCATCCCTG TTCCATTCTT ACAGTGCTTT 
ATGGGAGAGA AGTGTCAACG GTTTCCTCAC AACGAGTTTG TTCAGCTGAT CGGTGCATTG 
TTGGCCATCT GCTGCATGAT TTTCCTGGGC TTTGCTGATG ATGTTCTGAA CCTGCGATGG 
AGACACAAAC TTCTGCTGCC CACCATGGCC TCTCTGCCTC TCCTCATGGT TTACTTCACT 
AACTTTGGCA ACACTGTCAT CGTCGTGCCA AAACCTTTCC GTCTCCTTTT GGGGATGCAT 
TTGGACCTGG GAATTCTGTA TTATGTGTAT ATGGGAATGT TGGCTGTGTT TTGCACAAAT 
GCGATCAACA TCTTGGCTGG CATCAATGGC ATTGAGTCTG GACAGGCTCT CTTCATATCA 
GGGTCCATCA TCCTCTTTAA TTTACTGGAA CTCAATGGAG ACTACAGAGA TGACCATGTT 
TTCTCCTTGT ACTTCATGAT TCCTTTTTTC TTCACCACAT TAGCTCTCTT CTACCACAAT 
TGGTATCCCT CTTCTGTGTT TGTGGGAGAC ACTTTCTGTT ACTTTGCTGG AATGACATTT 
GCAGTTGTCG GGATACTCGG CCACTTTAGC AAGACAATGC TGCTTTTCTT CATCCCTCAA 
GTCATCAACT TTATTTACTC ACTGCCTCAG CTCTTTCACA TCATTCCCTG CCCTAGACAT 
CGCCTGCCTA GGTTACAGTC AGACACAGGA AAGCTGGGCA TGAGCTACTC CAAATTCAAA 
CAGAAAGACC TTGGCAAATT AGGGCAACTT ATTCTAAAGG TGGCAGAAAA GTTATGGGTT 
CTGGATGTGC GTAGAGGGCA GGAAGGAGAT GATGAGTTTA TTGAGTGTAA CAATATGACC 
CTTATCAATC TGGTGCTGAA AATACTGGGA CCTACCCATG AGAGAACATT GACTGCAATC 
ATGCTGTTAA TGCAGGTTTT GGGGAGTGCT GTGGCATTTG GCATTCGTTA TCACCTTGTG 
CGTCTGTTCT ATGACGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

trappc11 is required for protein glycosylation in zebrafish and humans.
Molecular biology of the cell27(8)1220-34(2016 Apr)
DeRossi C,Vacaru A,Rafiq R,Cinaroglu A,Imrie D,Nayar S,Baryshnikova A,Milev MP,Stanga D,Kadakia D,Gao N,Chu J,Freeze HH,Lehrman MA,Sacher M,Sadler KC

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Characterization of a BAC library from channel catfish Ictalurus punctatus: indications of high levels of chromosomal reshuffling among teleost genomes.
Marine biotechnology (New York, N.Y.)9(6)701-11(2007 Nov-Dec)
Wang S,Xu P,Thorsen J,Zhu B,de Jong PJ,Waldbieser G,Kucuktas H,Liu Z

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dpagt1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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