CWC22 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	CWC22
Entrez Gene ID	566033
Full Name	CWC22 spliceosome-associated protein homolog (S. cerevisiae)
Synonyms	wu:fb13g08,wu:fi16b02,zgc:153452
General protein information	Preferred Names CWC22 spliceosome-associated protein homolog (S. cerevisiae) Names pre-mRNA-splicing factor CWC22 homolog fb13g08 fi16b02 nucampholin homolog
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001077569.2	NP_001071037.2	pre-mRNA-splicing factor CWC22 homolog
XM_017357702.1	XP_017213191.1	pre-mRNA-splicing factor CWC22 homolog isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CWC22	NP_065994.1
Mus musculus (house mouse)	Cwc22	NP_085037.2
Eremothecium gossypii	AGOS_AGL355W	NP_986312.1
Rattus norvegicus (Norway rat)	Cwc22	XP_006234487.1
Gallus gallus (chicken)	CWC22	NP_001026421.1
Schizosaccharomyces pombe (fission yeast)	cwf22	NP_596256.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007874	XP_317618.4
Kluyveromyces lactis	KLLA0C14025g	XP_452827.1
Bos taurus (cattle)	CWC22	XP_005202345.1
Danio rerio (zebrafish)	cwc22	NP_001071037.1
Macaca mulatta (Rhesus monkey)	CWC22	XP_001100369.1
Canis lupus familiaris (dog)	CWC22	XP_005640430.1
Saccharomyces cerevisiae (baker's yeast)	CWC22	NP_011794.3
Pan troglodytes (chimpanzee)	CWC22	XP_001157639.2
Rattus norvegicus (Norway rat)	LOC500684	NP_001041424.1
Xenopus tropicalis (tropical clawed frog)	cwc22	XP_004917800.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

CERKL knockdown causes retinal degeneration in zebrafish.
Riera M, Burguera D, Garcia-Fernàndez J, Gonzàlez-Duarte R
PloS one8(5)e64048(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CWC22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CWC22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa98495	NM_001077569.2 Latest version!	Danio rerio CWC22 spliceosome associated protein homolog (cwc22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa60264	XM_017357702.1 Latest version!	Danio rerio CWC22 spliceosome-associated protein homolog (S. cerevisiae) (cwc22), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CWC22 ( NM_001077569.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001071037.2 Danio rerio CWC22 spliceosome associated protein homolog (cwc22), mRNA.

Price & Availability↑

CloneID	ODa98495
Clone ID Related Accession (Same CDS sequence)	NM_001077569.2
Accession Version	NM_001077569.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555862400000
Organism	Danio rerio(zebrafish)
Product	pre-mRNA-splicing factor CWC22 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX470219.6 and EH544241.1. On Sep 1, 2015 this sequence version replaced NM_001077569.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC124357.1, GDQH01006946.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCTG AGTCAAACGA ATCTCAGACG GCAATGAATA TGGGGCCTAC ATCGAGAGAT 
GAGCCTCCCA GCACAGAAGA AATGGAGCAG CGACAGAGAA AGGCCAGCAC AAGTTCATCT 
GAGGATGGAG AGCATGAAGA TAATGACCGC AGGAGGGTTG TTGGGTCTCC AGGCTCCAGA 
GATGGAAGTC CACGAGTGGG CAGCCCCGTG GCGAGGGCAT CACCCAGAGC CAAACGTGAT 
CAGAAATCCA GCGACTCTGA CAGTGACAGC AGTGACAGTG ATGACGGTGT AATGCGTAAG 
ATTAGGAGCA GTGTCATGCA TATCAGGCGT ACCTCTGATG AAGAGACCAA AAACAGAGAA 
AGAAGCTCCT CACCTGACCG GCATGAAAAG AAGTCAAAAT CAAGATCCCG CTCAAGATCC 
CGCTCCAGAT CTCGCTCCAG ATCTCGCTCT CCTCGTGAAC GTTATAGAAG GGCTCGTAGG 
TCCAGGGAGC GAGAGCGGGA CCGTTATGGT GATCGGGAGC GTTACGAGCG GCGCTCCAGC 
AGAGAGAGGG ATTGGGAACA CAGGAGGAGG GGAAGGTCAG CCTCTCCAGA CAAAAATGAT 
AAACCTCCCA CTGAGGAGCC TCCAGTGAAG AAGAGGAAGG AAACGCTGGA CCCCATCCTA 
ACCCGCACCG GTGGGGCCTA TATCCCACCG GCCAAACTGC GGATGATGCA GGCTCAGATC 
ACTGACAAGA GCAGTTTGGA GTATCAGAGG ATGAGCTGGG AAGCCCTGAA GAAGTCCATC 
AATGGTCTGA TCAACAAAGT GAACGTTTCT AACATCGCCA ACATTATTCA GGAGCTGCTG 
CAGGAGAATA TTGTGAGGGG CAGGGGTCTT CTAGCCAGGT CTATACTTCA GGCTCAGGCT 
GCGTCTCCCA TCTTCACTCA TGTATACTCT GCAGTGGTGG CCATCATCAA CTCCAAGTTT 
CCTCAAATTG GAGAACTTAT CCTCAAGAGG CTTATCCTTA ATTTCCGGAA AGGTTACAGG 
AGGAATGATA AACAACAATG TCTCACAGCG TCCAAGTTCG TAGGTCATCT CATCAACCAG 
AACGTGGCTC ATGAAGTCCT CTGTCTGGAG ATGCTGACCC TGCTGTTGGA AAGACCTACT 
GATGACAGTG TGGAGGTGGC CATCAGCTTC CTAAAAGAGT GTGGTCTCAA ACTGACAGAG 
GTCTCTCCTA GAGGCATCAA CGCCATCTTT GAACGTCTGA GGAACATCTT GCATGAGTCA 
GAGATAGATA AGCGTGTACA GTACATGATT GAGGTGATGT TTGCCATCAG GAAGGATGGG 
TTTAAGGACC ATCCCATCAT TCCAGAAGGA CTTGACCTAG TGGAGGAAGA GGACCAGTTC 
ACACACATGC TTCCTCTAGA GGATGAATAC AACACAGAGG ACATCTTAAA TGTTTTCAAG 
TTGGATCCAA ACTTCCTGGA GAACGAGGAG AAGTACAAGA CAATTAAACG GGAAATTCTT 
GATGAAGGAA GCAGTGATTC TGGTGACGAT GCGGGCGGCA GTGGAGATGA TGAAGACGAT 
GATGAGGAAG ATGAAGAGGC GGCAGCTGGA GAAGAACAAG AGAAAGTCAC CATTTTCGAC 
CAGACAGAGG TGAACCTGGT CGCGTTTCGT AGAACGATTT ACCTCGCCAT CCAGTCAAGC 
TTGGACTTCG AAGAATGCGC CCACAAGTTG ATAAAAATGG ATTTCCCCGA GAGCCAGACG 
AAAGAGCTGT GCAACATGAT CCTGGACTGT TGTGCTCAGC AAAGAACATA TGAGAAGTTC 
TTTGGTCTTC TAGCTGGGAG GTTTTGCCTG CTGAAGAAAG AGTACATGGA GAGCTTTGAG 
GCAATTTTCC AGGAGCAGTA TGAAACAATC CACCGGCTGG AAACCAACAA ACTGCGTAAC 
GTTGCTCGAA TCTTTGCTCA TCTCCTCTAC ACAGACTCAG TTCCATGGAG TGTCCTTGAA 
TGCGTTAGAA TGAGCGAAGA CACAACAACA TCCTCCAGTC GAATCTTTGT GAAGATTTTG 
TTCCAGGAAC TTTGTGCTTA CATGGGTTTG CCAAAACTAA ATGAGAGATT GAAGGACACG 
ACTCTCCAGC CATTTTTTGA AGGCCTGTTT CCACGCGATA ACCCGAGGAA CACAAGATTC 
GCCATTAATT TCTTCACATC TATCGGCCTT GGAGGACTAA CGGATGAGCT GAGGGAACAT 
CTGAAAAACG CTCCTAAGAT GATCATGACC CAGAATCAGG AAGTTGAGTC GTCAGATTCT 
TCCTCATCCT CCTCATCCTC TTCAGACTCC TCTTCATCTT CAGGTTCCTC ATCTGAAAGT 
GACAGCAGTG AAAGCGACTC TGATTCGTCC AGTGACTCAG ACTCTTCTTC CAGCAGTGGC 
AGCTCTTCAG ACTCTGACTC CAGGCGCAAG AAGGCCTCAG GTAAGAAAAA GGACAAGAGC 
AAGAAATCCA GCAAGGCAGC AAACCAGCGA TCTCCCTTAG AGGAACGACC CACCAAGAGA 
CACGAGAACC GCCGTCAGGA CGCCAGCAAA GAGGACCGAC GTGGATCTGA TAAACACAAC 
AGAGATCCTC AGCGGCGCGG ACAACAGGAC GAGTCACCTC CTGCTCGACC GAGAGGAGAA 
CCAGAGCGCA TAAGAGGACA GAAAGAACCT CACAGACACG CTCAGGACCA CCAGGACAGA 
CCGACAGATT CAGGCAGGCA CAAGGACGAC GGCAAAAACT CCCGAGCGAA CAAGGACAAA 
GATAGACGGC GGAGCAGGGA GAAGGAGCCT CTGCGGCGGA GCAGGGACAG GTCAAAGTCA 
CGAGAGAGGA GCCGCAAAGA GATGGACTCC AGGGACTCGT ACGGAAACGG CTTAGAGAGG 
GCAGACAAAG AGAACCGCCA TTCTGACAGA TACAAAGAGT CCAGGAGGAA AGACGACAGG 
AGACACAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071037.2
CDS	32..2983
Translation	MAAESNESQTAMNMGPTSRDEPPSTEEMEQRQRKASTSSSEDGEHEDNDRRRVVGSPGSRDGSPRVGSPVARASPRAKRDQKSSDSDSDSSDSDDGVMRKIRSSVMHIRRTSDEETKNRERSSSPDRHEKKSKSRSRSRSRSRSRSRSRSPRERYRRARRSRERERDRYGDRERYERRSSRERDWEHRRRGRSASPDKNDKPPTEEPPVKKRKETLDPILTRTGGAYIPPAKLRMMQAQITDKSSLEYQRMSWEALKKSINGLINKVNVSNIANIIQELLQENIVRGRGLLARSILQAQAASPIFTHVYSAVVAIINSKFPQIGELILKRLILNFRKGYRRNDKQQCLTASKFVGHLINQNVAHEVLCLEMLTLLLERPTDDSVEVAISFLKECGLKLTEVSPRGINAIFERLRNILHESEIDKRVQYMIEVMFAIRKDGFKDHPIIPEGLDLVEEEDQFTHMLPLEDEYNTEDILNVFKLDPNFLENEEKYKTIKREILDEGSSDSGDDAGGSGDDEDDDEEDEEAAAGEEQEKVTIFDQTEVNLVAFRRTIYLAIQSSLDFEECAHKLIKMDFPESQTKELCNMILDCCAQQRTYEKFFGLLAGRFCLLKKEYMESFEAIFQEQYETIHRLETNKLRNVARIFAHLLYTDSVPWSVLECVRMSEDTTTSSSRIFVKILFQELCAYMGLPKLNERLKDTTLQPFFEGLFPRDNPRNTRFAINFFTSIGLGGLTDELREHLKNAPKMIMTQNQEVESSDSSSSSSSSSDSSSSSGSSSESDSSESDSDSSSDSDSSSSSGSSSDSDSRRKKASGKKKDKSKKSSKAANQRSPLEERPTKRHENRRQDASKEDRRGSDKHNRDPQRRGQQDESPPARPRGEPERIRGQKEPHRHAQDHQDRPTDSGRHKDDGKNSRANKDKDRRRSREKEPLRRSRDRSKSRERSRKEMDSRDSYGNGLERADKENRHSDRYKESRRKDDRRHR

Target ORF information:

RefSeq Version	NM_001077569.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio CWC22 spliceosome associated protein homolog (cwc22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077569.2

ORF Insert Sequence:

ATGGCGGCTG AGTCAAACGA ATCTCAGACG GCAATGAATA TGGGGCCTAC ATCGAGAGAT 
GAGCCTCCCA GCACAGAAGA AATGGAGCAG CGACAGAGAA AGGCCAGCAC AAGTTCATCT 
GAGGATGGAG AGCATGAAGA TAATGACCGC AGGAGGGTTG TTGGGTCTCC AGGCTCCAGA 
GATGGAAGTC CACGAGTGGG CAGCCCCGTG GCGAGGGCAT CACCCAGAGC CAAACGTGAT 
CAGAAATCCA GCGACTCTGA CAGTGACAGC AGTGACAGTG ATGACGGTGT AATGCGTAAG 
ATTAGGAGCA GTGTCATGCA TATCAGGCGT ACCTCTGATG AAGAGACCAA AAACAGAGAA 
AGAAGCTCCT CACCTGACCG GCATGAAAAG AAGTCAAAAT CAAGATCCCG CTCAAGATCC 
CGCTCCAGAT CTCGCTCCAG ATCTCGCTCT CCTCGTGAAC GTTATAGAAG GGCTCGTAGG 
TCCAGGGAGC GAGAGCGGGA CCGTTATGGT GATCGGGAGC GTTACGAGCG GCGCTCCAGC 
AGAGAGAGGG ATTGGGAACA CAGGAGGAGG GGAAGGTCAG CCTCTCCAGA CAAAAATGAT 
AAACCTCCCA CTGAGGAGCC TCCAGTGAAG AAGAGGAAGG AAACGCTGGA CCCCATCCTA 
ACCCGCACCG GTGGGGCCTA TATCCCACCG GCCAAACTGC GGATGATGCA GGCTCAGATC 
ACTGACAAGA GCAGTTTGGA GTATCAGAGG ATGAGCTGGG AAGCCCTGAA GAAGTCCATC 
AATGGTCTGA TCAACAAAGT GAACGTTTCT AACATCGCCA ACATTATTCA GGAGCTGCTG 
CAGGAGAATA TTGTGAGGGG CAGGGGTCTT CTAGCCAGGT CTATACTTCA GGCTCAGGCT 
GCGTCTCCCA TCTTCACTCA TGTATACTCT GCAGTGGTGG CCATCATCAA CTCCAAGTTT 
CCTCAAATTG GAGAACTTAT CCTCAAGAGG CTTATCCTTA ATTTCCGGAA AGGTTACAGG 
AGGAATGATA AACAACAATG TCTCACAGCG TCCAAGTTCG TAGGTCATCT CATCAACCAG 
AACGTGGCTC ATGAAGTCCT CTGTCTGGAG ATGCTGACCC TGCTGTTGGA AAGACCTACT 
GATGACAGTG TGGAGGTGGC CATCAGCTTC CTAAAAGAGT GTGGTCTCAA ACTGACAGAG 
GTCTCTCCTA GAGGCATCAA CGCCATCTTT GAACGTCTGA GGAACATCTT GCATGAGTCA 
GAGATAGATA AGCGTGTACA GTACATGATT GAGGTGATGT TTGCCATCAG GAAGGATGGG 
TTTAAGGACC ATCCCATCAT TCCAGAAGGA CTTGACCTAG TGGAGGAAGA GGACCAGTTC 
ACACACATGC TTCCTCTAGA GGATGAATAC AACACAGAGG ACATCTTAAA TGTTTTCAAG 
TTGGATCCAA ACTTCCTGGA GAACGAGGAG AAGTACAAGA CAATTAAACG GGAAATTCTT 
GATGAAGGAA GCAGTGATTC TGGTGACGAT GCGGGCGGCA GTGGAGATGA TGAAGACGAT 
GATGAGGAAG ATGAAGAGGC GGCAGCTGGA GAAGAACAAG AGAAAGTCAC CATTTTCGAC 
CAGACAGAGG TGAACCTGGT CGCGTTTCGT AGAACGATTT ACCTCGCCAT CCAGTCAAGC 
TTGGACTTCG AAGAATGCGC CCACAAGTTG ATAAAAATGG ATTTCCCCGA GAGCCAGACG 
AAAGAGCTGT GCAACATGAT CCTGGACTGT TGTGCTCAGC AAAGAACATA TGAGAAGTTC 
TTTGGTCTTC TAGCTGGGAG GTTTTGCCTG CTGAAGAAAG AGTACATGGA GAGCTTTGAG 
GCAATTTTCC AGGAGCAGTA TGAAACAATC CACCGGCTGG AAACCAACAA ACTGCGTAAC 
GTTGCTCGAA TCTTTGCTCA TCTCCTCTAC ACAGACTCAG TTCCATGGAG TGTCCTTGAA 
TGCGTTAGAA TGAGCGAAGA CACAACAACA TCCTCCAGTC GAATCTTTGT GAAGATTTTG 
TTCCAGGAAC TTTGTGCTTA CATGGGTTTG CCAAAACTAA ATGAGAGATT GAAGGACACG 
ACTCTCCAGC CATTTTTTGA AGGCCTGTTT CCACGCGATA ACCCGAGGAA CACAAGATTC 
GCCATTAATT TCTTCACATC TATCGGCCTT GGAGGACTAA CGGATGAGCT GAGGGAACAT 
CTGAAAAACG CTCCTAAGAT GATCATGACC CAGAATCAGG AAGTTGAGTC GTCAGATTCT 
TCCTCATCCT CCTCATCCTC TTCAGACTCC TCTTCATCTT CAGGTTCCTC ATCTGAAAGT 
GACAGCAGTG AAAGCGACTC TGATTCGTCC AGTGACTCAG ACTCTTCTTC CAGCAGTGGC 
AGCTCTTCAG ACTCTGACTC CAGGCGCAAG AAGGCCTCAG GTAAGAAAAA GGACAAGAGC 
AAGAAATCCA GCAAGGCAGC AAACCAGCGA TCTCCCTTAG AGGAACGACC CACCAAGAGA 
CACGAGAACC GCCGTCAGGA CGCCAGCAAA GAGGACCGAC GTGGATCTGA TAAACACAAC 
AGAGATCCTC AGCGGCGCGG ACAACAGGAC GAGTCACCTC CTGCTCGACC GAGAGGAGAA 
CCAGAGCGCA TAAGAGGACA GAAAGAACCT CACAGACACG CTCAGGACCA CCAGGACAGA 
CCGACAGATT CAGGCAGGCA CAAGGACGAC GGCAAAAACT CCCGAGCGAA CAAGGACAAA 
GATAGACGGC GGAGCAGGGA GAAGGAGCCT CTGCGGCGGA GCAGGGACAG GTCAAAGTCA 
CGAGAGAGGA GCCGCAAAGA GATGGACTCC AGGGACTCGT ACGGAAACGG CTTAGAGAGG 
GCAGACAAAG AGAACCGCCA TTCTGACAGA TACAAAGAGT CCAGGAGGAA AGACGACAGG 
AGACACAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CWC22 ( XM_017357702.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213191.1 Danio rerio CWC22 spliceosome-associated protein homolog (S. cerevisiae) (cwc22), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa60264
Clone ID Related Accession (Same CDS sequence)	XM_017357702.1
Accession Version	XM_017357702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	pre-mRNA-splicing factor CWC22 homolog isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGATAC CAGAGGACTG GGAATACACG GGACCAGACA CAGAGACCAC CAGACAGGAG 
ACTAGCAGAA AGGACACAGA CACTGAAGGT CACAAGGATA GAGGTACACC TCCCAGCACA 
GAAGAAATGG AGCAGCGACA GAGAAAGGCC AGCACAAGTT CATCTGAGGA TGGAGAGCAT 
GAAGATAATG ACCGCAGGAG GGTTGTTGGG TCTCCAGGCT CCAGAGATGG AAGTCCACGA 
GTGGGCAGCC CCGTGGCGAG GGCATCACCC AGAGCCAAAC GTGATCAGAA ATCCAGCGAC 
TCTGACAGTG ACAGCAGTGA CAGTGATGAC GGTGTAATGC GTAAGATTAG GAGCAGTGTC 
ATGCATATCA GGCGTACCTC TGATGAAGAG ACCAAAAACA GAGAAAGAAG CTCCTCACCT 
GACCGGCATG AAAAGAAGTC AAAATCAAGA TCCCGCTCAA GATCCCGCTC CAGATCTCGC 
TCCAGATCTC GCTCTCCTCG TGAACGTTAT AGAAGGGCTC GTAGGTCCAG GGAGCGAGAG 
CGGGACCGTT ATGGTGATCG GGAGCGTTAC GAGCGGCGCT CCAGCAGAGA GAGGGATTGG 
GAACACAGGA GGAGGGGAAG GTCAGCCTCT CCAGACAAAA ATGATAAACC TCCCACTGAG 
GAGCCTCCAG TGAAGAAGAG GAAGGAAACG CTGGACCCCA TCCTAACCCG CACCGGTGGG 
GCCTATATCC CACCGGCCAA ACTGCGGATG ATGCAGGCTC AGATCACTGA CAAGAGCAGT 
TTGGAGTATC AGAGGATGAG CTGGGAAGCC CTGAAGAAGT CCATCAATGG TCTGATCAAC 
AAAGTGAACG TTTCTAACAT CGCCAACATT ATTCAGGAGC TGCTGCAGGA GAATATTGTG 
AGGGGCAGGG GTCTTCTAGC CAGGTCTATA CTTCAGGCTC AGGCTGCGTC TCCCATCTTC 
ACTCATGTAT ACTCTGCAGT GGTGGCCATC ATCAACTCCA AGTTTCCTCA AATTGGAGAA 
CTTATCCTCA AGAGGCTTAT CCTTAATTTC CGGAAAGGTT ACAGGAGGAA TGATAAACAA 
CAATGTCTCA CAGCGTCCAA GTTCGTAGGT CATCTCATCA ACCAGAACGT GGCTCATGAA 
GTCCTCTGTC TGGAGATGCT GACCCTGCTG TTGGAAAGAC CTACTGATGA CAGTGTGGAG 
GTGGCCATCA GCTTCCTAAA AGAGTGTGGT CTCAAACTGA CAGAGGTCTC TCCTAGAGGC 
ATCAACGCCA TCTTTGAACG TCTGAGGAAC ATCTTGCATG AGTCAGAGAT AGATAAGCGT 
GTACAGTACA TGATTGAGGT GATGTTTGCC ATCAGGAAGG ATGGGTTTAA GGACCATCCC 
ATCATTCCAG AAGGACTTGA CCTAGTGGAG GAAGAGGACC AGTTCACACA CATGCTTCCT 
CTAGAGGATG AATACAACAC AGAGGACATC TTAAATGTTT TCAAGTTGGA TCCAAACTTC 
CTGGAGAACG AGGAGAAGTA CAAGACAATT AAACGGGAAA TTCTTGATGA AGGAAGCAGT 
GATTCTGGTG ACGATGCGGG CGGCAGTGGA GATGATGAAG ACGATGATGA GGAAGATGAA 
GAGGCGGCAG CTGGAGAAGA ACAAGAGAAA GTCACCATTT TCGACCAGAC AGAGGTGAAC 
CTGGTCGCGT TTCGTAGAAC GATTTACCTC GCCATCCAGT CAAGCTTGGA CTTCGAAGAA 
TGCGCCCACA AGTTGATAAA AATGGATTTC CCCGAGAGCC AGACGAAAGA GCTGTGCAAC 
ATGATCCTGG ACTGTTGTGC TCAGCAAAGA ACATATGAGA AGTTCTTTGG TCTTCTAGCT 
GGGAGGTTTT GCCTGCTGAA GAAAGAGTAC ATGGAGAGCT TTGAGGCAAT TTTCCAGGAG 
CAGTATGAAA CAATCCACCG GCTGGAAACC AACAAACTGC GTAACGTTGC TCGAATCTTT 
GCTCATCTCC TCTACACAGA CTCAGTTCCA TGGAGTGTCC TTGAATGCGT TAGAATGAGC 
GAAGACACAA CAACATCCTC CAGTCGAATC TTTGTGAAGA TTTTGTTCCA GGAACTTTGT 
GCTTACATGG GTTTGCCAAA ACTAAATGAG AGATTGAAGG ACACGACTCT CCAGCCATTT 
TTTGAAGGCC TGTTTCCACG CGATAACCCG AGGAACACAA GATTCGCCAT TAATTTCTTC 
ACATCTATCG GCCTTGGAGG ACTAACGGAT GAGCTGAGGG AACATCTGAA AAACGCTCCT 
AAGATGATCA TGACCCAGAA TCAGGAAGTT GAGTCGTCAG ATTCTTCCTC ATCCTCCTCA 
TCCTCTTCAG ACTCCTCTTC ATCTTCAGGT TCCTCATCTG AAAGTGACAG CAGTGAAAGC 
GACTCTGATT CGTCCAGTGA CTCAGACTCT TCTTCCAGCA GTGGCAGCTC TTCAGACTCT 
GACTCCAGGC GCAAGAAGGC CTCAGGTAAG AAAAAGGACA AGAGCAAGAA ATCCAGCAAG 
GCAGCAAACC AGCGATCTCC CTTAGAGGAA CGACCCACCA AGAGACACGA GAACCGCCGT 
CAGGACGCCA GCAAAGAGGA CCGACGTGGA TCTGATAAAC ACAACAGAGA TCCTCAGCGG 
CGCGGACAAC AGGACGAGTC ACCTCCTGCT CGACCGAGAG GAGAACCAGA GCGCATAAGA 
GGACAGAAAG AACCTCACAG ACACGCTCAG GACCACCAGG ACAGACCGAC AGATTCAGGC 
AGGCACAAGG ACGACGGCAA AAACTCCCGA GCGAACAAGG ACAAAGATAG ACGGCGGAGC 
AGGGAGAAGG AGCCTCTGCG GCGGAGCAGG GACAGGTCAA AGTCACGAGA GAGGAGCCGC 
AAAGAGATGG ACTCCAGGGA CTCGTACGGA AACGGCTTAG AGAGGGCAGA CAAAGAGAAC 
CGCCATTCTG ACAGATACAA AGAGTCCAGG AGGAAAGACG ACAGGAGACA CAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213191.1
CDS	3..2999
Translation	MQIPEDWEYTGPDTETTRQETSRKDTDTEGHKDRGTPPSTEEMEQRQRKASTSSSEDGEHEDNDRRRVVGSPGSRDGSPRVGSPVARASPRAKRDQKSSDSDSDSSDSDDGVMRKIRSSVMHIRRTSDEETKNRERSSSPDRHEKKSKSRSRSRSRSRSRSRSRSPRERYRRARRSRERERDRYGDRERYERRSSRERDWEHRRRGRSASPDKNDKPPTEEPPVKKRKETLDPILTRTGGAYIPPAKLRMMQAQITDKSSLEYQRMSWEALKKSINGLINKVNVSNIANIIQELLQENIVRGRGLLARSILQAQAASPIFTHVYSAVVAIINSKFPQIGELILKRLILNFRKGYRRNDKQQCLTASKFVGHLINQNVAHEVLCLEMLTLLLERPTDDSVEVAISFLKECGLKLTEVSPRGINAIFERLRNILHESEIDKRVQYMIEVMFAIRKDGFKDHPIIPEGLDLVEEEDQFTHMLPLEDEYNTEDILNVFKLDPNFLENEEKYKTIKREILDEGSSDSGDDAGGSGDDEDDDEEDEEAAAGEEQEKVTIFDQTEVNLVAFRRTIYLAIQSSLDFEECAHKLIKMDFPESQTKELCNMILDCCAQQRTYEKFFGLLAGRFCLLKKEYMESFEAIFQEQYETIHRLETNKLRNVARIFAHLLYTDSVPWSVLECVRMSEDTTTSSSRIFVKILFQELCAYMGLPKLNERLKDTTLQPFFEGLFPRDNPRNTRFAINFFTSIGLGGLTDELREHLKNAPKMIMTQNQEVESSDSSSSSSSSSDSSSSSGSSSESDSSESDSDSSSDSDSSSSSGSSSDSDSRRKKASGKKKDKSKKSSKAANQRSPLEERPTKRHENRRQDASKEDRRGSDKHNRDPQRRGQQDESPPARPRGEPERIRGQKEPHRHAQDHQDRPTDSGRHKDDGKNSRANKDKDRRRSREKEPLRRSRDRSKSRERSRKEMDSRDSYGNGLERADKENRHSDRYKESRRKDDRRHR

Target ORF information:

RefSeq Version	XM_017357702.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio CWC22 spliceosome-associated protein homolog (S. cerevisiae) (cwc22), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357702.1

ORF Insert Sequence:

ATGCAGATAC CAGAGGACTG GGAATACACG GGACCAGACA CAGAGACCAC CAGACAGGAG 
ACTAGCAGAA AGGACACAGA CACTGAAGGT CACAAGGATA GAGGTACACC TCCCAGCACA 
GAAGAAATGG AGCAGCGACA GAGAAAGGCC AGCACAAGTT CATCTGAGGA TGGAGAGCAT 
GAAGATAATG ACCGCAGGAG GGTTGTTGGG TCTCCAGGCT CCAGAGATGG AAGTCCACGA 
GTGGGCAGCC CCGTGGCGAG GGCATCACCC AGAGCCAAAC GTGATCAGAA ATCCAGCGAC 
TCTGACAGTG ACAGCAGTGA CAGTGATGAC GGTGTAATGC GTAAGATTAG GAGCAGTGTC 
ATGCATATCA GGCGTACCTC TGATGAAGAG ACCAAAAACA GAGAAAGAAG CTCCTCACCT 
GACCGGCATG AAAAGAAGTC AAAATCAAGA TCCCGCTCAA GATCCCGCTC CAGATCTCGC 
TCCAGATCTC GCTCTCCTCG TGAACGTTAT AGAAGGGCTC GTAGGTCCAG GGAGCGAGAG 
CGGGACCGTT ATGGTGATCG GGAGCGTTAC GAGCGGCGCT CCAGCAGAGA GAGGGATTGG 
GAACACAGGA GGAGGGGAAG GTCAGCCTCT CCAGACAAAA ATGATAAACC TCCCACTGAG 
GAGCCTCCAG TGAAGAAGAG GAAGGAAACG CTGGACCCCA TCCTAACCCG CACCGGTGGG 
GCCTATATCC CACCGGCCAA ACTGCGGATG ATGCAGGCTC AGATCACTGA CAAGAGCAGT 
TTGGAGTATC AGAGGATGAG CTGGGAAGCC CTGAAGAAGT CCATCAATGG TCTGATCAAC 
AAAGTGAACG TTTCTAACAT CGCCAACATT ATTCAGGAGC TGCTGCAGGA GAATATTGTG 
AGGGGCAGGG GTCTTCTAGC CAGGTCTATA CTTCAGGCTC AGGCTGCGTC TCCCATCTTC 
ACTCATGTAT ACTCTGCAGT GGTGGCCATC ATCAACTCCA AGTTTCCTCA AATTGGAGAA 
CTTATCCTCA AGAGGCTTAT CCTTAATTTC CGGAAAGGTT ACAGGAGGAA TGATAAACAA 
CAATGTCTCA CAGCGTCCAA GTTCGTAGGT CATCTCATCA ACCAGAACGT GGCTCATGAA 
GTCCTCTGTC TGGAGATGCT GACCCTGCTG TTGGAAAGAC CTACTGATGA CAGTGTGGAG 
GTGGCCATCA GCTTCCTAAA AGAGTGTGGT CTCAAACTGA CAGAGGTCTC TCCTAGAGGC 
ATCAACGCCA TCTTTGAACG TCTGAGGAAC ATCTTGCATG AGTCAGAGAT AGATAAGCGT 
GTACAGTACA TGATTGAGGT GATGTTTGCC ATCAGGAAGG ATGGGTTTAA GGACCATCCC 
ATCATTCCAG AAGGACTTGA CCTAGTGGAG GAAGAGGACC AGTTCACACA CATGCTTCCT 
CTAGAGGATG AATACAACAC AGAGGACATC TTAAATGTTT TCAAGTTGGA TCCAAACTTC 
CTGGAGAACG AGGAGAAGTA CAAGACAATT AAACGGGAAA TTCTTGATGA AGGAAGCAGT 
GATTCTGGTG ACGATGCGGG CGGCAGTGGA GATGATGAAG ACGATGATGA GGAAGATGAA 
GAGGCGGCAG CTGGAGAAGA ACAAGAGAAA GTCACCATTT TCGACCAGAC AGAGGTGAAC 
CTGGTCGCGT TTCGTAGAAC GATTTACCTC GCCATCCAGT CAAGCTTGGA CTTCGAAGAA 
TGCGCCCACA AGTTGATAAA AATGGATTTC CCCGAGAGCC AGACGAAAGA GCTGTGCAAC 
ATGATCCTGG ACTGTTGTGC TCAGCAAAGA ACATATGAGA AGTTCTTTGG TCTTCTAGCT 
GGGAGGTTTT GCCTGCTGAA GAAAGAGTAC ATGGAGAGCT TTGAGGCAAT TTTCCAGGAG 
CAGTATGAAA CAATCCACCG GCTGGAAACC AACAAACTGC GTAACGTTGC TCGAATCTTT 
GCTCATCTCC TCTACACAGA CTCAGTTCCA TGGAGTGTCC TTGAATGCGT TAGAATGAGC 
GAAGACACAA CAACATCCTC CAGTCGAATC TTTGTGAAGA TTTTGTTCCA GGAACTTTGT 
GCTTACATGG GTTTGCCAAA ACTAAATGAG AGATTGAAGG ACACGACTCT CCAGCCATTT 
TTTGAAGGCC TGTTTCCACG CGATAACCCG AGGAACACAA GATTCGCCAT TAATTTCTTC 
ACATCTATCG GCCTTGGAGG ACTAACGGAT GAGCTGAGGG AACATCTGAA AAACGCTCCT 
AAGATGATCA TGACCCAGAA TCAGGAAGTT GAGTCGTCAG ATTCTTCCTC ATCCTCCTCA 
TCCTCTTCAG ACTCCTCTTC ATCTTCAGGT TCCTCATCTG AAAGTGACAG CAGTGAAAGC 
GACTCTGATT CGTCCAGTGA CTCAGACTCT TCTTCCAGCA GTGGCAGCTC TTCAGACTCT 
GACTCCAGGC GCAAGAAGGC CTCAGGTAAG AAAAAGGACA AGAGCAAGAA ATCCAGCAAG 
GCAGCAAACC AGCGATCTCC CTTAGAGGAA CGACCCACCA AGAGACACGA GAACCGCCGT 
CAGGACGCCA GCAAAGAGGA CCGACGTGGA TCTGATAAAC ACAACAGAGA TCCTCAGCGG 
CGCGGACAAC AGGACGAGTC ACCTCCTGCT CGACCGAGAG GAGAACCAGA GCGCATAAGA 
GGACAGAAAG AACCTCACAG ACACGCTCAG GACCACCAGG ACAGACCGAC AGATTCAGGC 
AGGCACAAGG ACGACGGCAA AAACTCCCGA GCGAACAAGG ACAAAGATAG ACGGCGGAGC 
AGGGAGAAGG AGCCTCTGCG GCGGAGCAGG GACAGGTCAA AGTCACGAGA GAGGAGCCGC 
AAAGAGATGG ACTCCAGGGA CTCGTACGGA AACGGCTTAG AGAGGGCAGA CAAAGAGAAC 
CGCCATTCTG ACAGATACAA AGAGTCCAGG AGGAAAGACG ACAGGAGACA CAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

CERKL knockdown causes retinal degeneration in zebrafish.
PloS one8(5)e64048(2013)
Riera M,Burguera D,Garcia-Fernàndez J,Gonzàlez-Duarte R

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CWC22 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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