PLEKHG2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLEKHG2
Entrez Gene ID	565988
Full Name	pleckstrin homology domain containing, family G (with RhoGef domain) member 2
General protein information	Preferred Names pleckstrin homology domain containing, family G (with RhoGef domain) member 2 Names pleckstrin homology domain-containing family G member 2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009291706.2	XP_009289981.1	pleckstrin homology domain-containing family G member 2 isoform X2
XM_689253.9	XP_694345.6	pleckstrin homology domain-containing family G member 2
XM_009291708.2	XP_009289983.1	pleckstrin homology domain-containing family G member 2 isoform X4
XM_689253.8	XP_694345.6	pleckstrin homology domain-containing family G member 2 isoform X1
XM_021472942.1	XP_021328617.1	pleckstrin homology domain-containing family G member 2
XM_009291707.1	XP_009289982.1	pleckstrin homology domain-containing family G member 2 isoform X3

Pathways from BioSystems
Gene Ontology

The following PLEKHG2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLEKHG2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa44768	XM_009291708.2 Latest version!	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (plekhg2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa44767	XM_009291707.1 Latest version!	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (plekhg2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa145746	XM_021472942.1 Latest version!	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (plekhg2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa20412	XM_689253.9 Latest version!	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (plekhg2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa20413	XM_009291706.2 Latest version!	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (plekhg2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
ODa20412	XM_689253.8	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (plekhg2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLEKHG2 ( XM_009291708.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009289983.1 Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (plekhg2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa44768
Clone ID Related Accession (Same CDS sequence)	XM_009291708.2
Accession Version	XM_009291708.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family G member 2 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291708.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAAG GTGCGGGACG CAACAGCCAG AAAAAAAACA GCAACCAAGC TGCCAAGCGC 
CCCTCCTCTG TGTCCTCGCT GAGTGGGATT GTGAGCCGCA TGGCCTCCTC AGGAGGCGCA 
TCCCGCGGGA GCTGCACCTC CGTTAACACA GTGTGCTCTG ACAGTGACCG TGCTGCTAGT 
CTAAGCTCCT CTGCCTCCTC TGCCTCGCTG CAGGATGGAC ACTCTTCGTC TTCGTCCTCA 
TCGCTCCCCT ACGGAGCAGT GCCAGCCTAC CCTGGGCCAC AGCATAATGG TTCTGATATC 
AGCCTGGACC TCACCCCTCT CTCCCTGCTG GCGGGAGCCG GTGCCCTCTC CACTTCCACA 
ACAACACTGC CCAAACTTAC TCGCCTGGAG AGGGTGGCGT TAGAGATACT GGAGACCGAA 
CAGGCCTATG TGAGGGACCT CAAGAGCATT GTGGAGGACT ATCTTGGCTG CATTATTGAC 
TGTGGCGACT TGCCGCTCAA ACCCGAAGAG GTCAGCACCC TCTTCTGCAA CATCGAGGAC 
ATCTATGAGT TTAACAGTGA GCTTTTGGAA GATCTGGAGC GCAGTCCGCA TGCGGCAGCC 
ATAGCAGAGT GCTTTGTGGA GAGGAGTGAA GCTTTTGACA TTTACACCAT ATACTGCATG 
AATTACCCCA ACTCAGTGAC CGTCCTGCGG GACTGCATGA AGAACGAGAG CCTGGTGCGC 
TTCTTCCAGG AAAGACAGGC CACCTTGTGT CACTCGCTGC CTCTGGAGAC CTACCTGCTA 
AAACCAGTGC AGAGAATCCT TAAATACCAC CTCCTGCTGC AGGAGCTGTC CAAACACTTT 
GATAAAAGTG ACCCTGGCTA TGAGGTGGTG GAAGATGCCA TTATAACTAT GACAGCAGTG 
GCCTGGTACA TCAATGACAT GAAGAGGAAG CAGGAACATG CAATCAGATT GCAGGAGATT 
GAGAGCTTGC TGCTGAACTG GACGGGTCCT GACCTCAGTG GTTTTGGAGA GCTGGTTCTT 
GAAGGTTCCT TCCGGGTTCA ACGCGTGAAA AAGGAAAGAG CTTTCTTTCT TTTTGACAAA 
ATGCTTCTTA TTGCCAAGAA GAGGCTGGAT CATTTCATAT ACAGTACTCA CATATTTTGC 
TGTAATCTGC TCTTAGTGGA AAATATGAAG GATCCCCTCT GTTTTAAGGT GTCTGACCAA 
ACAATCCCAA AACAACAACA TGTTGTTCAG GCTAAAAACC AGGAGGAGAA GCGACTGTGG 
CTACATTACC TCAAGAGATT GATCGTAGAA AACCATCCTG CATCCTTGCC TCAAAAAGCG 
AGGCAGGTTC TTGGTGACAA CTTCTGTCAG TCTCCACAGT TTGATCAGGA ACCAATTAGA 
AAGCCAATGC CGTCACCACG ACTGGATGAT GTTCATAACT ACCACAGAGG AAGAAGGCAA 
TCAGAGCCAC CAGAATTCAT CTACACCCCT GAAAAAGCCA AGAAAAGCCT TCCCATGCTG 
TTAGAGGGTA ACCTGCCATA CAAACGTGGA AGAAGACAAT CTGCGCCTGC CAAAGATATA 
GAAGCTGCAT TTCAGCAAAG CGGTTCTTTA AAGAGTTCTC AAGCTGGCAG TGAAGGAGAA 
TTGTGCCCAC AGGCTGATAG TATGGGCTCT TCAGGCAGTA CCAGTACTCT GGCTTCCTCA 
GTCATTGAAG TTGAATCTGG ACGTGATGAT CTGGCCTTAT GTCAAGATGA AGATGATATG 
ACACCACTCC CTCCACCACC AACACTGTCT ATTACTGAAG AAATCATGGA ATTCATTAAC 
CAGAGCCGTG CTCGGCAGGG CATGACAGAG TTGACATCTG ATAGGTGTCA GTTGATTCCA 
GAGACCTTAG AAGTTCAGCC ATTGGAAGGA CAGGAGTCAG AGGAATCCAA ACCACTTATA 
GCTGAGATGG ATGAAAATAC ACATGCGATC ATTAATGTCC AAAGATCCGA TGCAGAGAAA 
ACTGAATCAG TTCAAGACGA GGACAGCCAT CCATCCTGTG AAGAAAATAT TTCTCAACCA 
AATGAGCCAA AGGGAGTATC ACCAACTCTG CATGTCTCTA CTGAGGAAAA TCAATTAGAA 
GAGCTGACAT TCCAAGAGGC CAGTTGTGGT GAATCATGCA AATCAGAGAC CTTAAGTGAA 
GGAGAGAAAG ATAATGAGAA CTCTGTTAGT AAAAGTGAAA ATAAATCAGC TAAATCTGCA 
AATGAGACAG CATGTGAGGC CAATTCACAT TGTCCTCTTA TTGATACTTC AGAGAAAGAA 
TCTGAAAGCT CTCCCAAAAT AGAACCTGAG CAAAAACTGA CCAAAAGTGA CAAGCAGATT 
ATTGAGAAGA TTCGCAGCTA TTATGAGGCA GCGGAGACAG GAGTTGAGGA AGGACAGTTT 
GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289983.1
CDS	545..2950
Translation	MPEGAGRNSQKKNSNQAAKRPSSVSSLSGIVSRMASSGGASRGSCTSVNTVCSDSDRAASLSSSASSASLQDGHSSSSSSSLPYGAVPAYPGPQHNGSDISLDLTPLSLLAGAGALSTSTTTLPKLTRLERVALEILETEQAYVRDLKSIVEDYLGCIIDCGDLPLKPEEVSTLFCNIEDIYEFNSELLEDLERSPHAAAIAECFVERSEAFDIYTIYCMNYPNSVTVLRDCMKNESLVRFFQERQATLCHSLPLETYLLKPVQRILKYHLLLQELSKHFDKSDPGYEVVEDAIITMTAVAWYINDMKRKQEHAIRLQEIESLLLNWTGPDLSGFGELVLEGSFRVQRVKKERAFFLFDKMLLIAKKRLDHFIYSTHIFCCNLLLVENMKDPLCFKVSDQTIPKQQHVVQAKNQEEKRLWLHYLKRLIVENHPASLPQKARQVLGDNFCQSPQFDQEPIRKPMPSPRLDDVHNYHRGRRQSEPPEFIYTPEKAKKSLPMLLEGNLPYKRGRRQSAPAKDIEAAFQQSGSLKSSQAGSEGELCPQADSMGSSGSTSTLASSVIEVESGRDDLALCQDEDDMTPLPPPPTLSITEEIMEFINQSRARQGMTELTSDRCQLIPETLEVQPLEGQESEESKPLIAEMDENTHAIINVQRSDAEKTESVQDEDSHPSCEENISQPNEPKGVSPTLHVSTEENQLEELTFQEASCGESCKSETLSEGEKDNENSVSKSENKSAKSANETACEANSHCPLIDTSEKESESSPKIEPEQKLTKSDKQIIEKIRSYYEAAETGVEEGQFV

Target ORF information:

RefSeq Version	XM_009291708.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (plekhg2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291708.2

ORF Insert Sequence:

ATGCCTGAAG GTGCGGGACG CAACAGCCAG AAAAAAAACA GCAACCAAGC TGCCAAGCGC 
CCCTCCTCTG TGTCCTCGCT GAGTGGGATT GTGAGCCGCA TGGCCTCCTC AGGAGGCGCA 
TCCCGCGGGA GCTGCACCTC CGTTAACACA GTGTGCTCTG ACAGTGACCG TGCTGCTAGT 
CTAAGCTCCT CTGCCTCCTC TGCCTCGCTG CAGGATGGAC ACTCTTCGTC TTCGTCCTCA 
TCGCTCCCCT ACGGAGCAGT GCCAGCCTAC CCTGGGCCAC AGCATAATGG TTCTGATATC 
AGCCTGGACC TCACCCCTCT CTCCCTGCTG GCGGGAGCCG GTGCCCTCTC CACTTCCACA 
ACAACACTGC CCAAACTTAC TCGCCTGGAG AGGGTGGCGT TAGAGATACT GGAGACCGAA 
CAGGCCTATG TGAGGGACCT CAAGAGCATT GTGGAGGACT ATCTTGGCTG CATTATTGAC 
TGTGGCGACT TGCCGCTCAA ACCCGAAGAG GTCAGCACCC TCTTCTGCAA CATCGAGGAC 
ATCTATGAGT TTAACAGTGA GCTTTTGGAA GATCTGGAGC GCAGTCCGCA TGCGGCAGCC 
ATAGCAGAGT GCTTTGTGGA GAGGAGTGAA GCTTTTGACA TTTACACCAT ATACTGCATG 
AATTACCCCA ACTCAGTGAC CGTCCTGCGG GACTGCATGA AGAACGAGAG CCTGGTGCGC 
TTCTTCCAGG AAAGACAGGC CACCTTGTGT CACTCGCTGC CTCTGGAGAC CTACCTGCTA 
AAACCAGTGC AGAGAATCCT TAAATACCAC CTCCTGCTGC AGGAGCTGTC CAAACACTTT 
GATAAAAGTG ACCCTGGCTA TGAGGTGGTG GAAGATGCCA TTATAACTAT GACAGCAGTG 
GCCTGGTACA TCAATGACAT GAAGAGGAAG CAGGAACATG CAATCAGATT GCAGGAGATT 
GAGAGCTTGC TGCTGAACTG GACGGGTCCT GACCTCAGTG GTTTTGGAGA GCTGGTTCTT 
GAAGGTTCCT TCCGGGTTCA ACGCGTGAAA AAGGAAAGAG CTTTCTTTCT TTTTGACAAA 
ATGCTTCTTA TTGCCAAGAA GAGGCTGGAT CATTTCATAT ACAGTACTCA CATATTTTGC 
TGTAATCTGC TCTTAGTGGA AAATATGAAG GATCCCCTCT GTTTTAAGGT GTCTGACCAA 
ACAATCCCAA AACAACAACA TGTTGTTCAG GCTAAAAACC AGGAGGAGAA GCGACTGTGG 
CTACATTACC TCAAGAGATT GATCGTAGAA AACCATCCTG CATCCTTGCC TCAAAAAGCG 
AGGCAGGTTC TTGGTGACAA CTTCTGTCAG TCTCCACAGT TTGATCAGGA ACCAATTAGA 
AAGCCAATGC CGTCACCACG ACTGGATGAT GTTCATAACT ACCACAGAGG AAGAAGGCAA 
TCAGAGCCAC CAGAATTCAT CTACACCCCT GAAAAAGCCA AGAAAAGCCT TCCCATGCTG 
TTAGAGGGTA ACCTGCCATA CAAACGTGGA AGAAGACAAT CTGCGCCTGC CAAAGATATA 
GAAGCTGCAT TTCAGCAAAG CGGTTCTTTA AAGAGTTCTC AAGCTGGCAG TGAAGGAGAA 
TTGTGCCCAC AGGCTGATAG TATGGGCTCT TCAGGCAGTA CCAGTACTCT GGCTTCCTCA 
GTCATTGAAG TTGAATCTGG ACGTGATGAT CTGGCCTTAT GTCAAGATGA AGATGATATG 
ACACCACTCC CTCCACCACC AACACTGTCT ATTACTGAAG AAATCATGGA ATTCATTAAC 
CAGAGCCGTG CTCGGCAGGG CATGACAGAG TTGACATCTG ATAGGTGTCA GTTGATTCCA 
GAGACCTTAG AAGTTCAGCC ATTGGAAGGA CAGGAGTCAG AGGAATCCAA ACCACTTATA 
GCTGAGATGG ATGAAAATAC ACATGCGATC ATTAATGTCC AAAGATCCGA TGCAGAGAAA 
ACTGAATCAG TTCAAGACGA GGACAGCCAT CCATCCTGTG AAGAAAATAT TTCTCAACCA 
AATGAGCCAA AGGGAGTATC ACCAACTCTG CATGTCTCTA CTGAGGAAAA TCAATTAGAA 
GAGCTGACAT TCCAAGAGGC CAGTTGTGGT GAATCATGCA AATCAGAGAC CTTAAGTGAA 
GGAGAGAAAG ATAATGAGAA CTCTGTTAGT AAAAGTGAAA ATAAATCAGC TAAATCTGCA 
AATGAGACAG CATGTGAGGC CAATTCACAT TGTCCTCTTA TTGATACTTC AGAGAAAGAA 
TCTGAAAGCT CTCCCAAAAT AGAACCTGAG CAAAAACTGA CCAAAAGTGA CAAGCAGATT 
ATTGAGAAGA TTCGCAGCTA TTATGAGGCA GCGGAGACAG GAGTTGAGGA AGGACAGTTT 
GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHG2 ( XM_009291707.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009289982.1 Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (plekhg2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa44767
Clone ID Related Accession (Same CDS sequence)	XM_009291707.1
Accession Version	XM_009291707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family G member 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCAGC GGAGACAGGA GTTGAGGAAG GACAGTTTGT TTGAGTCTGA GAGTGGCCGC 
TCAGACACTG TTGACACTGA CTCAGCATTT TTAATACTCC CTGTATCTGA CCAAGCTGAC 
CAGACTTTCA GCCATCAGGA ATCTGTTGAT GCAGACATTC CTCCCTCACA TTCAGATGCT 
GACCATCAAG TGGAAGAAAT TGACGGCAAG ACCTCAATGA AAAAGGATGA AGATGTTTGT 
GATTTCAGTA TGAAACTGTG GAAGGAGAAT GAAAAGACTG GCCAAGAGTC TCCAAGCAGT 
CCGAAAGTCC CCATCTCAAG AGAGAACAGC TGTGGTGAAC ATACAGATGC TCCTGAGAAA 
TTGTCCGTAA ATTGCATAAC AACCCAAGTA CAGCATAAAC AATCGGAGAG TTTACAATGT 
GATCCAGACG CACCAGACAA ATCTAATCTG CAGGCCACAG AGACAGCAGA TGCATCACTT 
CAATGTGCAA GTAAAACTAG ATCTAGGATG TCTTCTAATG TAAGCCTGGA TAGCCTTCCC 
ATTCAGATTA AAGTTGGCAG ATGGTCTCGT CATGGCAAGG TGGTTACATG TAGCCAAACC 
CTTTATGAAG GAATGGCCGA GGTACCAGAT TTAGGATTTT TTGAGAGTGG ACCAGTCAAT 
CAGTGCTTGG TGGAAAACTC AGAAAAGATT TTAAATAAAG TGCAAATGCT TGCACGCATG 
TACACTGCCA AGGCGAGCAG CATGAAGGTA CCGCTGCATC AGAAAAAAAC ACGAGTGAGT 
AGGGGAGCAT GGTCAATGGA TGGAAAAGGG AGCACTTCAC CTAAACCTCA GCAAGTAAAG 
CTGCACCAGG GGGATATGAG GATAAGCCAG TCTGAAGAAG AATCTTTGGA AACTACACCT 
ACTGAGCCTT TTGGTCATAT AATTTTAAGG GAGAAGCTGT CTACAACTTA TCACCAAGAA 
AATGATTGCA ATCTTATTGG CCCGCTAGAT GAGATTTCAG AAACTGGATC TAATTCACTG 
TCTTTTTCTT CTGCTGATTC CTCTTTCACT TCGGCTCAAA TACTAGCAAC TGTTACAGAA 
TCAAAGGTCT CTGTGATATC AGACCATACA GACAGGTCAT TAGAAATGAC AGAGTCGAAA 
CTACATGAAA AAGAACAATC CGTACCACTT CCAGTTTACT CTGGAACACC AGTCCAAGCT 
GAGTCAGTAG TCCCATCCAA AATCTGTGGG TTGCCATTGG TTGAGCATGC TAGCAGTGTA 
CTACAGTCCA TTCAGGAGAA CCATTTAGAT GTTGGGGACA AATTAAATAC AGTATATAGA 
ATTGCAGAAA GACAGGTATT GCAAGAGGAT AAACCTGCTT TTGTAAACAA TTATTGTCCA 
ATTAGTGATG TTTTTATTGA CCCACTAGTC AATAAACATG TAACTAAAGA GACTCACAGT 
GAGGAGAAAA AAGACTCTGT TGTACAGCAC TGTGAAAGTA TATTGTCTAT AGATGATGAA 
CGAGATGAAA TGGATAAAGG ACATTTGAAA TCCAACCAGT CTGATACTGA AATGACTAAC 
ATGGAGGAAC TAACACATAT AGTGTCCTCC GTTAGTGATG AAAGCACATA TCCTCCATTA 
CACTTAGCAG CCTCCTTAAA AAACAGTGTT CCATCCACTT TGATAATCTC TGAGGAATCA 
AAACCTTTGG CCCAGGTGCA GGATTTAACT AGCCCTACGC ATCTGGTGAC TCTGCTTGGT 
TCATGTAGCA AGTGTAGTCC ACAGGAAGAT AAATCTAAAG AAGACATTAA TTCAATGACT 
TTGAGCCCAT GCTCAACCCC ACTTGGTAGA CCTCTTTTAG ATGAGCTACC AAGGTTTACA 
AATCAAAGAC CCTCCAATCT GCCTTCCACC ACAGGAACGA GAAGTCCCTT CAAAAATTGG 
AATTCATCAT CTCCAGTTGC TCAAAGAGTG CCACACACTT CACTGCGAAC AGAGGATCAC 
ACTCAAGATA TTACTTCTTA TGGCCTTTCT CCTCACAAGC AGTCTTCATC CCAGGCTTTT 
AATGATAAGT CTTCAATCAC TCCCTTGGGT GGTGAACATA AATTAGATAC AAAACCACCC 
TCTGCCTTTA GTCCAAGTCT CCGAATGAGG TCACCCTCTC CAATTAGAGG TACACAGAGC 
TCCAGACCTA CTTCAACAAC CTCAGCTCTT ACAAAGTCTC TTGCTGCCTC CTGCATCAGT 
CAGACGATTT CTCAAAGCAT GGCCAAGAGA GGTGCACATA TTCAGACTGT GTCCCAACCT 
GTAAGATCCT CTCCTCTACC CACATCTTCC GTAAGATTAC GGACGCCATC ACCAAAACCC 
ATCACCTTGG ACTCAAATGT AGAGTCAGGA TTTAGGGCAA TATCCAGCCC TGGGGTAGGA 
AGTCAACCCC CAAGGTCTTG TCCATCCCCA TTCAGATCCA ACAGTCTTCG ATCTAGTCCA 
GCAACAGTCC AGTCCCCTCC TCCTTATCGT AGCCAAAGGT CAATATCTCC AGCACCTCCA 
GTTAGCCTCC ATTCAACTCC ATCTGTTAAC AGCCCAACCT CCACTGCTCT TGAACAACCT 
TCGTTCAGCA GTTTAAATTG TAATAATAAC AATAATAACA GCTTAAGTAA TAATGGGTGG 
GTCGCTAATA ACAAAAAAAC AGCACCATCA GCTGTAAGTG GACTCCCTCA TTTCCATGAT 
TCCCAGCGAA CAACCTCACA CAATAGGGTG GCTCGTCCTT TCCCATCGTC TGAGCCTAGT 
TCCCGTGTAC AGTCCCCTTC CATTTGCCTC CAATCTCCAA TCACCCGAAT CTGCTCTCCT 
CCACCTGCTC CCAACCATTC CAGTCATCTG GTAACAAAGC CACCCAACCC CAGAGCATCC 
AGACAAGGTG GCTACTTTAC ACCTTTATCT TTGGAAATTT CAAGATCAAC ATCAGCTTGT 
TCAATATCTC CTTGCGGGAG TCCCAGAATC ACCTCTCCTC CCCCTATTGG CATTCCTACA 
AATGTTTGGG GCTATGCTAA TCCACAACCA AGGAATCCAT TAGTAACAAG TGCATCGTCT 
CATACAAAAT TGGAAACAAA CTCTACTCCA AGAGGCTCTA GAATCATCTC GCCCACCCCA 
ACAGGATCAT CATCCTATTC AGCCAACTCT CAGAGTCAGA GGAGGTTACG AGGAACCAGT 
TTGCCCTTTG TTGTGATAGG GGATAGCCTT CCAAGTCCAA CTAGACACGA ACGCAGATCT 
TGGGCTGAAA GTGGACGGTG GTCAGTGGGT TCAGATCTAG GTTTAATAAG TCCTCGTGGG 
GGTTCATACA GTGGTAGCCC GTCTTCTGTT AGCCCTGGTC CTCTTTCACC AGTCAGACTA 
ACAGCCGAAA GGACAAGTGG AAAACACTTT ACAGGCATTT CCTGGCCAGA TGTACACGAT 
CTCTTGATTA AATACACAGA GCCCAATACT GAAGCAAATG CTTTATCCTG CACCGAGATA 
GAGCCAGGTG ACATGGACGG TACAGAACCT ACATGTCGGA GTACCCTTAT TTGTCCATAT 
GTTGCTCCAG CCAGCCCCAT TGAGAGAGAC ACTCCCATTC AGTGCCAGAC CGAGGCTAAA 
ACAGAAGACT GTGTTCCTAA TCAAGGGTCT AAAACAACAT TAAAGACCAG TTATGCCACC 
ACTGTCAATT TGCAGATTGC TGGGAGTGGG AGGATTGCAT CCTTTAGCAA TGCACAAGTC 
AGCCTGACTC AAACTCTGAC CCCTGTGGCA GACAGTCAGA GCAGGAGAAG AGTCAGCATC 
AACAGCTGCA ACTTCTCTAT GCAGAACTGC AAAAGGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289982.1
CDS	98..3919
Translation	MRQRRQELRKDSLFESESGRSDTVDTDSAFLILPVSDQADQTFSHQESVDADIPPSHSDADHQVEEIDGKTSMKKDEDVCDFSMKLWKENEKTGQESPSSPKVPISRENSCGEHTDAPEKLSVNCITTQVQHKQSESLQCDPDAPDKSNLQATETADASLQCASKTRSRMSSNVSLDSLPIQIKVGRWSRHGKVVTCSQTLYEGMAEVPDLGFFESGPVNQCLVENSEKILNKVQMLARMYTAKASSMKVPLHQKKTRVSRGAWSMDGKGSTSPKPQQVKLHQGDMRISQSEEESLETTPTEPFGHIILREKLSTTYHQENDCNLIGPLDEISETGSNSLSFSSADSSFTSAQILATVTESKVSVISDHTDRSLEMTESKLHEKEQSVPLPVYSGTPVQAESVVPSKICGLPLVEHASSVLQSIQENHLDVGDKLNTVYRIAERQVLQEDKPAFVNNYCPISDVFIDPLVNKHVTKETHSEEKKDSVVQHCESILSIDDERDEMDKGHLKSNQSDTEMTNMEELTHIVSSVSDESTYPPLHLAASLKNSVPSTLIISEESKPLAQVQDLTSPTHLVTLLGSCSKCSPQEDKSKEDINSMTLSPCSTPLGRPLLDELPRFTNQRPSNLPSTTGTRSPFKNWNSSSPVAQRVPHTSLRTEDHTQDITSYGLSPHKQSSSQAFNDKSSITPLGGEHKLDTKPPSAFSPSLRMRSPSPIRGTQSSRPTSTTSALTKSLAASCISQTISQSMAKRGAHIQTVSQPVRSSPLPTSSVRLRTPSPKPITLDSNVESGFRAISSPGVGSQPPRSCPSPFRSNSLRSSPATVQSPPPYRSQRSISPAPPVSLHSTPSVNSPTSTALEQPSFSSLNCNNNNNNSLSNNGWVANNKKTAPSAVSGLPHFHDSQRTTSHNRVARPFPSSEPSSRVQSPSICLQSPITRICSPPPAPNHSSHLVTKPPNPRASRQGGYFTPLSLEISRSTSACSISPCGSPRITSPPPIGIPTNVWGYANPQPRNPLVTSASSHTKLETNSTPRGSRIISPTPTGSSSYSANSQSQRRLRGTSLPFVVIGDSLPSPTRHERRSWAESGRWSVGSDLGLISPRGGSYSGSPSSVSPGPLSPVRLTAERTSGKHFTGISWPDVHDLLIKYTEPNTEANALSCTEIEPGDMDGTEPTCRSTLICPYVAPASPIERDTPIQCQTEAKTEDCVPNQGSKTTLKTSYATTVNLQIAGSGRIASFSNAQVSLTQTLTPVADSQSRRRVSINSCNFSMQNCKRL

Target ORF information:

RefSeq Version	XM_009291707.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (plekhg2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291707.1

ORF Insert Sequence:

ATGAGGCAGC GGAGACAGGA GTTGAGGAAG GACAGTTTGT TTGAGTCTGA GAGTGGCCGC 
TCAGACACTG TTGACACTGA CTCAGCATTT TTAATACTCC CTGTATCTGA CCAAGCTGAC 
CAGACTTTCA GCCATCAGGA ATCTGTTGAT GCAGACATTC CTCCCTCACA TTCAGATGCT 
GACCATCAAG TGGAAGAAAT TGACGGCAAG ACCTCAATGA AAAAGGATGA AGATGTTTGT 
GATTTCAGTA TGAAACTGTG GAAGGAGAAT GAAAAGACTG GCCAAGAGTC TCCAAGCAGT 
CCGAAAGTCC CCATCTCAAG AGAGAACAGC TGTGGTGAAC ATACAGATGC TCCTGAGAAA 
TTGTCCGTAA ATTGCATAAC AACCCAAGTA CAGCATAAAC AATCGGAGAG TTTACAATGT 
GATCCAGACG CACCAGACAA ATCTAATCTG CAGGCCACAG AGACAGCAGA TGCATCACTT 
CAATGTGCAA GTAAAACTAG ATCTAGGATG TCTTCTAATG TAAGCCTGGA TAGCCTTCCC 
ATTCAGATTA AAGTTGGCAG ATGGTCTCGT CATGGCAAGG TGGTTACATG TAGCCAAACC 
CTTTATGAAG GAATGGCCGA GGTACCAGAT TTAGGATTTT TTGAGAGTGG ACCAGTCAAT 
CAGTGCTTGG TGGAAAACTC AGAAAAGATT TTAAATAAAG TGCAAATGCT TGCACGCATG 
TACACTGCCA AGGCGAGCAG CATGAAGGTA CCGCTGCATC AGAAAAAAAC ACGAGTGAGT 
AGGGGAGCAT GGTCAATGGA TGGAAAAGGG AGCACTTCAC CTAAACCTCA GCAAGTAAAG 
CTGCACCAGG GGGATATGAG GATAAGCCAG TCTGAAGAAG AATCTTTGGA AACTACACCT 
ACTGAGCCTT TTGGTCATAT AATTTTAAGG GAGAAGCTGT CTACAACTTA TCACCAAGAA 
AATGATTGCA ATCTTATTGG CCCGCTAGAT GAGATTTCAG AAACTGGATC TAATTCACTG 
TCTTTTTCTT CTGCTGATTC CTCTTTCACT TCGGCTCAAA TACTAGCAAC TGTTACAGAA 
TCAAAGGTCT CTGTGATATC AGACCATACA GACAGGTCAT TAGAAATGAC AGAGTCGAAA 
CTACATGAAA AAGAACAATC CGTACCACTT CCAGTTTACT CTGGAACACC AGTCCAAGCT 
GAGTCAGTAG TCCCATCCAA AATCTGTGGG TTGCCATTGG TTGAGCATGC TAGCAGTGTA 
CTACAGTCCA TTCAGGAGAA CCATTTAGAT GTTGGGGACA AATTAAATAC AGTATATAGA 
ATTGCAGAAA GACAGGTATT GCAAGAGGAT AAACCTGCTT TTGTAAACAA TTATTGTCCA 
ATTAGTGATG TTTTTATTGA CCCACTAGTC AATAAACATG TAACTAAAGA GACTCACAGT 
GAGGAGAAAA AAGACTCTGT TGTACAGCAC TGTGAAAGTA TATTGTCTAT AGATGATGAA 
CGAGATGAAA TGGATAAAGG ACATTTGAAA TCCAACCAGT CTGATACTGA AATGACTAAC 
ATGGAGGAAC TAACACATAT AGTGTCCTCC GTTAGTGATG AAAGCACATA TCCTCCATTA 
CACTTAGCAG CCTCCTTAAA AAACAGTGTT CCATCCACTT TGATAATCTC TGAGGAATCA 
AAACCTTTGG CCCAGGTGCA GGATTTAACT AGCCCTACGC ATCTGGTGAC TCTGCTTGGT 
TCATGTAGCA AGTGTAGTCC ACAGGAAGAT AAATCTAAAG AAGACATTAA TTCAATGACT 
TTGAGCCCAT GCTCAACCCC ACTTGGTAGA CCTCTTTTAG ATGAGCTACC AAGGTTTACA 
AATCAAAGAC CCTCCAATCT GCCTTCCACC ACAGGAACGA GAAGTCCCTT CAAAAATTGG 
AATTCATCAT CTCCAGTTGC TCAAAGAGTG CCACACACTT CACTGCGAAC AGAGGATCAC 
ACTCAAGATA TTACTTCTTA TGGCCTTTCT CCTCACAAGC AGTCTTCATC CCAGGCTTTT 
AATGATAAGT CTTCAATCAC TCCCTTGGGT GGTGAACATA AATTAGATAC AAAACCACCC 
TCTGCCTTTA GTCCAAGTCT CCGAATGAGG TCACCCTCTC CAATTAGAGG TACACAGAGC 
TCCAGACCTA CTTCAACAAC CTCAGCTCTT ACAAAGTCTC TTGCTGCCTC CTGCATCAGT 
CAGACGATTT CTCAAAGCAT GGCCAAGAGA GGTGCACATA TTCAGACTGT GTCCCAACCT 
GTAAGATCCT CTCCTCTACC CACATCTTCC GTAAGATTAC GGACGCCATC ACCAAAACCC 
ATCACCTTGG ACTCAAATGT AGAGTCAGGA TTTAGGGCAA TATCCAGCCC TGGGGTAGGA 
AGTCAACCCC CAAGGTCTTG TCCATCCCCA TTCAGATCCA ACAGTCTTCG ATCTAGTCCA 
GCAACAGTCC AGTCCCCTCC TCCTTATCGT AGCCAAAGGT CAATATCTCC AGCACCTCCA 
GTTAGCCTCC ATTCAACTCC ATCTGTTAAC AGCCCAACCT CCACTGCTCT TGAACAACCT 
TCGTTCAGCA GTTTAAATTG TAATAATAAC AATAATAACA GCTTAAGTAA TAATGGGTGG 
GTCGCTAATA ACAAAAAAAC AGCACCATCA GCTGTAAGTG GACTCCCTCA TTTCCATGAT 
TCCCAGCGAA CAACCTCACA CAATAGGGTG GCTCGTCCTT TCCCATCGTC TGAGCCTAGT 
TCCCGTGTAC AGTCCCCTTC CATTTGCCTC CAATCTCCAA TCACCCGAAT CTGCTCTCCT 
CCACCTGCTC CCAACCATTC CAGTCATCTG GTAACAAAGC CACCCAACCC CAGAGCATCC 
AGACAAGGTG GCTACTTTAC ACCTTTATCT TTGGAAATTT CAAGATCAAC ATCAGCTTGT 
TCAATATCTC CTTGCGGGAG TCCCAGAATC ACCTCTCCTC CCCCTATTGG CATTCCTACA 
AATGTTTGGG GCTATGCTAA TCCACAACCA AGGAATCCAT TAGTAACAAG TGCATCGTCT 
CATACAAAAT TGGAAACAAA CTCTACTCCA AGAGGCTCTA GAATCATCTC GCCCACCCCA 
ACAGGATCAT CATCCTATTC AGCCAACTCT CAGAGTCAGA GGAGGTTACG AGGAACCAGT 
TTGCCCTTTG TTGTGATAGG GGATAGCCTT CCAAGTCCAA CTAGACACGA ACGCAGATCT 
TGGGCTGAAA GTGGACGGTG GTCAGTGGGT TCAGATCTAG GTTTAATAAG TCCTCGTGGG 
GGTTCATACA GTGGTAGCCC GTCTTCTGTT AGCCCTGGTC CTCTTTCACC AGTCAGACTA 
ACAGCCGAAA GGACAAGTGG AAAACACTTT ACAGGCATTT CCTGGCCAGA TGTACACGAT 
CTCTTGATTA AATACACAGA GCCCAATACT GAAGCAAATG CTTTATCCTG CACCGAGATA 
GAGCCAGGTG ACATGGACGG TACAGAACCT ACATGTCGGA GTACCCTTAT TTGTCCATAT 
GTTGCTCCAG CCAGCCCCAT TGAGAGAGAC ACTCCCATTC AGTGCCAGAC CGAGGCTAAA 
ACAGAAGACT GTGTTCCTAA TCAAGGGTCT AAAACAACAT TAAAGACCAG TTATGCCACC 
ACTGTCAATT TGCAGATTGC TGGGAGTGGG AGGATTGCAT CCTTTAGCAA TGCACAAGTC 
AGCCTGACTC AAACTCTGAC CCCTGTGGCA GACAGTCAGA GCAGGAGAAG AGTCAGCATC 
AACAGCTGCA ACTTCTCTAT GCAGAACTGC AAAAGGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHG2 ( XM_021472942.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021328617.1 Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (plekhg2), mRNA.

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CloneID	ODa145746
Clone ID Related Accession (Same CDS sequence)	XM_021472942.1
Accession Version	XM_021472942.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family G member 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394946.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAAG GTGCGGGACG CAACAGCCAG AAAAAAAACA GCAACCAAGC TGCCAAGCGC 
CCCTCCTCTG TGTCCTCGCT GAGTGGGATT GTGAGCCGCA TGGCCTCCTC AGGAGGCGCA 
TCCCGCGGGA GCTGCACCTC CGTTAACACA GTGTGCTCTG ACAGTGACCG TGCTGCTAGT 
CTAAGCTCCT CTGCCTCCTC TGCCTCGCTG CAGGATGGAC ACTCTTCGTC TTCGTCCTCA 
TCGCTCCCCT ACGGAGCAGT GCCAGCCTAC CCTGGGCCAC AGCATAATGG TTCTGATATC 
AGCCTGGACC TCACCCCTCT CTCCCTGCTG GCGGGAGCCG GTGCCCTCTC CACTTCCACA 
ACAACACTGC CCAAACTTAC TCGCCTGGAG AGGGTGGCGT TAGAGATACT GGAGACCGAA 
CAGGCCTATG TGAGGGACCT CAAGAGCATT GTGGAGGACT ATCTTGGCTG CATTATTGAC 
TGTGGCGACT TGCCGCTCAA ACCCGAAGAG GTCAGCACCC TCTTCTGCAA CATCGAGGAC 
ATCTATGAGT TTAACAGTGA GCTTTTGGAA GATCTGGAGC GCAGTCCGCA TGCGGCAGCC 
ATAGCAGAGT GCTTTGTGGA GAGGAGTGAA GCTTTTGACA TTTACACCAT ATACTGCATG 
AATTACCCCA ACTCAGTGAC CGTCCTGCGG GACTGCATGA AGAACGAGAG CCTGGTGCGC 
TTCTTCCAGG AAAGACAGGC CACCTTGTGT CACTCGCTGC CTCTGGAGAC CTACCTGCTA 
AAACCAGTGC AGAGAATCCT TAAATACCAC CTCCTGCTGC AGGAGCTGTC CAAACACTTT 
GATAAAAGTG ACCCTGGCTA TGAGGTGGTG GAAGATGCCA TTATAACTAT GACAGCAGTG 
GCCTGGTACA TCAATGACAT GAAGAGGAAG CAGGAACATG CAATCAGACT GCAGGAGATT 
GAGAGCTTGC TGCTGAACTG GACGGGTCCT GACCTCAGTG GTTTTGGAGA GCTGGTTCTT 
GAAGGTTCCT TCCGGGTTCA ACGCGTGAAA AAGGAAAGAG CTTTCTTTCT TTTTGACAAA 
ATGCTTCTTA TTGCCAAGAA GAGGCTAGAT CATTTCATAT ACAGTACTCA CATATTTTGC 
TGTAATCTGC TCTTAGTGGA AAATATGAAG GATCCCCTCT GTTTTAAGGT GTCTGACCAA 
ACAATCCCAA AACAACAACA TGTTGTTCAG GCTAAAAACC AGGAGGAGAA ACGACTGTGG 
CTACATTACC TCAAGAGATT GATCGTAGAA AACCATCCTG CATCCTTGCC TCAAAAAGCG 
AGGCAGGTTC TTGGTGACAA CTTCTGTCAG TCTCCACAGT TTGATCAGGA ACCAATTAGA 
AAGCCAATGC CGTCACCACG ACTGGATGAT GTTCATAACT ACCACAGAGG AAGAAGGCAA 
TCAGAGCCAC CAGAATTCAT CTACACCCCT GAAAAAGCCA AGAAAAGCCT TCCCATGCTG 
TTAGAGGGTA ACCTGCCATA CAAACGTGGA AGAAGACAGT CCGCGCCTGC CAAAGATATA 
GAAGCTGCAT TTCAGCAAAG CGGTTCTTTA AAGAGTTCTC AAGCTGGCAG TGAAGGGGAA 
TTGTGCCCAC AGGCTGATAG TATGGGCTCT TCAGGCAGTA CTAGTACTCT GGCATCCTCA 
GTCATTGAAG TTGAATCTGG ACGTGATGAT CTCGCCTTGT GTCAAGATGA AGATGATATG 
ACACCACTCC CTCCACCACC AACACTGTCT ATTACTGAAG AAATCATGGA GTTCATTAAC 
CAGAGCCGTG CTCGGCAGGG CATGACAGAG TTGATATCTG ATAGGTGTCA GTTGATTCCA 
GAGACCTTAG AAGTTCAGCC ATTGGAAGCA CAGGAGTCAG AGGAATCCAA ACCACTTATA 
GCTGAGATGG ATGAAAATAC ACATGCGATC ATTAATGTCC ACAGATCTGA TGCAGAGAAA 
ACTGAATCAG TCCAAGACGA GGACAGCCAT CCTTCCTGTG AAGAAAATAT TTCTCAACCA 
AATGAGCCGA AGGGAGTATC ACCAACTCTG CATGTCTCTA CTGAGGAAAA TCAATTAGAA 
GAGCTGACAT TCCAAGAGGC CAGTTGTGGT GAATCATGCA AATCAGAGAC CTTAAGTGAA 
GGAGAGAAAG ATAATGAGAA CTCTGTTAGT AAAAGTGAAA ATAAATCAGA TAAATCTGCA 
AATGAGACAG CATGTGAGGC CAATTCACAT TGTCCTCTTT TTGATACTTC AGAGAAAGCA 
TCTGAAAGCT CTCCCAAAAT AGAACCTGAG CAAAAACTGA CCAAAAGTGA CAAGCAGATT 
ATTGAGAAGA TTCGCAGCTA TTATGAGGCA GCGGAGACAG GAGTTGAGGA AGGTCAGTCT 
ACACGAAGAA ACAGCTTTTC TCATATTCCT GCAGGATTAG TGAAGGATTC TGTAACACGA 
TTCAATGTTT GTGTTCGTCA GGACAGTTTG TTTGAGTCTG AGAGTGGCCG CTCAGACACT 
GTTGACACTG ACTCAGCATT TTTAATACTC CCTGTATCTG ACCAAGCTGA CCAGACTTTC 
AGCCATCAGG AATCTGTTGA TGCAGACATT CCTCCCTCAC ATTCAGATGC TGACCATCAA 
GTGGAAGAAA TTGACGGCAA GACCTCAATG AAAAAGGATG AAGATGTTTG TGATTTCAGT 
ATGAAACTGT GGAAGGAGAA TGAAAGGACT GGCCAAGAGT CTCCAAGCAG TCCGAAAGTC 
CCCATCTCAA GAGAGAACAG CTGTGGTGAA CATACAGATG CTCCTGAGAA ATTGTCCGTA 
AATTGCATAA CAACCCAAGT ACAGCATAAA CAATCGGAGA GTTTACAATG TGATCCAGAC 
GCACCAGACA AATCTAATCT GCAGGTCACA GAGACAGCAC ATGCATCACT TCAATGTGCT 
AGTAAAACTA GACCTAGGAT GTCTTCTAAT GGAAGCCTGG ATAGCCTTCC CAGTCAGATT 
AAAGTTGGCA GATGGTCTCG TCATGGCAAG GTGGTTACAT GTAGCCAAAC CCTTTATGAA 
GGAATGGCCG AGGTACCAGA TTTAGGATTT TTTGAGGGTG GACCAGTCAA TCAGTGCTTG 
GTGGAAAACT CAGAAAAGAT TTTAAATAAA GTGCAGATGC TTGCACGCAT GTACACTGCC 
AAGGCAAGCA GCATGAAGGT ACCGCTACAT CAGAAAAAAA CACGAGTGAG TAGGGGAGCA 
TGGTCAGTGG ATGGAAAAGG GAGCACTTCA CCCAAACCCC AGCAAGTAAA GCTTCACCAG 
GGGGATATGA GGATAAGCCA GTCTGCAGAA GAATCTTTGG AAACTACACC TACTGAGCCT 
TTTGGTCATA TAATTTTAAG GGAGAAGCTA TCTACAACTT ATCACCAAGA AAATGATTGC 
AATCTTATTG GCCCGCTAGA TGAGATTTCA GAAACTGGAT CTAATTCACT GTCTTTTTCT 
TCTGCTGATT CCTCTTTCAC TTCGGCTCAA ATACTAGCAA CTGTTACAGA ATCACAGGTC 
TCTGTGATAT CAGACCATAC AGACAAGTCA TTAGAAATGA CAGAGTTGAA ACTACATGAA 
AAAGATCAAT CCGTACCACT TCCAGTATAC TCTGGAACAC CAGTCCAAGC TGAGTCAGTA 
GTCCCATCCA AAATCTGTGG GTTGCCATTG GTTGAGCATG CTAGCAGTGT ACTACCGTCC 
ATTCAGGAGA ACCATTTAGC TGTTGGGGAC AAATCAAATC CAGTATATAA AATTGCAGAA 
AGACAGGTAT TAAAAGAGGA TAAACCTGCT TTTGTGAACA ATTATTGTCC AATTAGTGAT 
GTTTTTATTG ACCCACTGGT CAATAAACAT GTACCTAAAG AGATTCACAG TGAGGAGAAA 
AAAGACTCTG TTGTACAGCA CTGTGAAAGT ATATTGTCTA TAGATGATGA ACGAGATGAA 
ATGGATAAAG GACATTTGAA ATCCAACCAG TCTGATACTG AAATGACTAA CATGGAGGAA 
CTAACACATA TAGTGTCCTC CGTTAGTGAT GAAAGCACAT ATCCTCCATT ACACTTAGCA 
GCCTCCTTAA AAAATAGTGT TCCATCCACT TTGATAATCT CTGAGGAATC AAAACCTTTG 
GCCCAGGTGC AGGATTCAAC TAGCCCTACG CATCTAGTGA CTCTGCTTGG TTCATGTAGT 
AAGTGTAGTC CACAGGAAGA TAAATCTAAA GAAGACATTA ATTCAATGAC TCTGAGCCCA 
TGCTCAACCC CACTTGGTAG ACCTCTTTTA GATGAGCTAC CAAGGTTTAC AAATCAAAGA 
CCCTCCAATC TGCCTTCCAC CACAGGAACG AGAAGTCCCT TCATAAATTG GAATTCATCG 
TCTCCAGTTG CTCAAAGAGT GCCACACACT TCACTGCGAA CAGAGGATCA CACTCAAGAT 
ATTACTTCTT ATGGCCTTTC TCCTCACAAG CAGTCTTCAT CCCAGGCTTT TAATGATAAG 
TCTTCAATTA CTCCCTTGGG TGGTGGACAT AAATTGGATA CAAAACCACC CTCTGCCTTT 
AGCCCAAGTC TCCGAATGAG GTCACCCTCT CCAATTAGAG GTACACAGAG CTCCAGACCT 
ACTTCAACAA CCTCAGCTCT TACAAAGTCT CTTGCTGCCT CCTGCATAAG TCAGACGATT 
TCTCAAAGCA TGGCCAAGAG AGGTGCACAT ATTCAGACTG TGTCCCCACC TGTAAGATCC 
TCTCCTCTAC CCACATCTTC CGTAAGATTA AGGACGCCAT CACCAAAACC CATCACCTTG 
GACTCAAATG TAGAGTCAGG ATTTAGGGCA ATATCCAGCC CTGGGGTAGG AAGTCAACCC 
CCAAGGTCTT GTCCATCCCC ATTCAGATCC AACAGTCTTC GATCTAGTCC AGCAACAGTT 
CAGTCCCCTC CTCCTTATCG TAGCCAAAGG TCAATATCTC CAGCACCTCC AGTTAGCCTC 
CATTCAACTC CATCTGTTAA CAGCCCAACC TCCACAACTC TTGAACAACC TTCGTTCAGC 
AGTTTAAATT GTAATAATAA CAATAATAAC AGCTTAAGTA ATAATGGGTG GGTTGCTAAT 
AACAAAAAAA CAGCACCATC AACTGTCAGT GGACTCCCTC ATTTCCATGA TTCCCAACGA 
ACAACCTCAC ACAATAGGGT GGCTCGTCCT TTCCCATCGT CTGAGCCTAG TTCCCGTGTA 
CAGTCCCCTT CCATTTGCCT CCAATCTCCA ATCACCCGAA TCTGCTCTCC TCCACCTGCT 
CCCAACCATT CCAGTCATCT GGTAACAAAG CCACCCAACC CCAGAGCATC CAGACAAGGT 
GGCTACTTTA CACCTTTATC TTTGGAAATT TCAAGATCAA CATCAGCTTG TTCAATATCT 
CCTTGCGGGA GTCCCAGAAT CACCTCTCCT CCCCCTATTG GCATTCCTAC AAATGTTTGG 
GGCTATGCTA ATCCACAACC AAGGAATCCA TTAGTAACAA GTGCATCGTC TCATACAAAA 
TTGGAAACAA ACTCTACACC AAGAGGCTCT AGAATCATCT CGCCCACCCC AACAGGATCA 
TCATCCTATT CAGCCAACTC TCAGAGTCAG AGGAGGTTAC GAGGAACCAG TTTGCCCTTT 
GTTGTGATAG GGGATAGCCT TCCAAGTCCA ACTAGACACG AACGCAGGTC TTGGGCTGAA 
AGTGGACGGT GGTCAGTGGG TTCAGATCTG GGTTTAATAA GTCCTCGTGA GGGTTCATAC 
AGCGGTAGCC CGTCTTCTGT TAGCCCTGGT CCTCTTTCAC CAGTCAGACT AACAGCCGAA 
AGGACAAGTG GAAAACACTT TACAGGCGTT TCCTGGCCAG ATGTACACGA TCTGTTGATT 
AAATACACAG AGCCCAATAC TGAAGCAAAT GCTTTATCCT GCACCGAGAT AGAGCCAGGT 
GACATAGACG GTACAGAACC TACATGTCGG AGTACCCTTA TTTGTCCATA TGTTGCTCCA 
GCCAGCCCTG TTGAGAGAGA CACTCCCATC CAGTGCCAAA CCGAGGCTAA AACAGAAGAC 
TGTGTTCCTA ATCAAGGGTC TAAAACAACA TTAAAGACCA GTTATGCCAC CACTGTCAAT 
TTGCAGATTG CTGGGAGTGG GAGGATTGCA TCCTTTAGCA ATGCGCAAGT CAGCCTGACT 
CAAACTCTGA CCCCTGTGGC AGACAGTCAG AGCAGGAGAA GAGTCAGCAT CAATAGCTGC 
AACTTCTCTA TGCAGAACTG CAAAAGGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328617.1
CDS	566..6838
Translation	MPEGAGRNSQKKNSNQAAKRPSSVSSLSGIVSRMASSGGASRGSCTSVNTVCSDSDRAASLSSSASSASLQDGHSSSSSSSLPYGAVPAYPGPQHNGSDISLDLTPLSLLAGAGALSTSTTTLPKLTRLERVALEILETEQAYVRDLKSIVEDYLGCIIDCGDLPLKPEEVSTLFCNIEDIYEFNSELLEDLERSPHAAAIAECFVERSEAFDIYTIYCMNYPNSVTVLRDCMKNESLVRFFQERQATLCHSLPLETYLLKPVQRILKYHLLLQELSKHFDKSDPGYEVVEDAIITMTAVAWYINDMKRKQEHAIRLQEIESLLLNWTGPDLSGFGELVLEGSFRVQRVKKERAFFLFDKMLLIAKKRLDHFIYSTHIFCCNLLLVENMKDPLCFKVSDQTIPKQQHVVQAKNQEEKRLWLHYLKRLIVENHPASLPQKARQVLGDNFCQSPQFDQEPIRKPMPSPRLDDVHNYHRGRRQSEPPEFIYTPEKAKKSLPMLLEGNLPYKRGRRQSAPAKDIEAAFQQSGSLKSSQAGSEGELCPQADSMGSSGSTSTLASSVIEVESGRDDLALCQDEDDMTPLPPPPTLSITEEIMEFINQSRARQGMTELISDRCQLIPETLEVQPLEAQESEESKPLIAEMDENTHAIINVHRSDAEKTESVQDEDSHPSCEENISQPNEPKGVSPTLHVSTEENQLEELTFQEASCGESCKSETLSEGEKDNENSVSKSENKSDKSANETACEANSHCPLFDTSEKASESSPKIEPEQKLTKSDKQIIEKIRSYYEAAETGVEEGQSTRRNSFSHIPAGLVKDSVTRFNVCVRQDSLFESESGRSDTVDTDSAFLILPVSDQADQTFSHQESVDADIPPSHSDADHQVEEIDGKTSMKKDEDVCDFSMKLWKENERTGQESPSSPKVPISRENSCGEHTDAPEKLSVNCITTQVQHKQSESLQCDPDAPDKSNLQVTETAHASLQCASKTRPRMSSNGSLDSLPSQIKVGRWSRHGKVVTCSQTLYEGMAEVPDLGFFEGGPVNQCLVENSEKILNKVQMLARMYTAKASSMKVPLHQKKTRVSRGAWSVDGKGSTSPKPQQVKLHQGDMRISQSAEESLETTPTEPFGHIILREKLSTTYHQENDCNLIGPLDEISETGSNSLSFSSADSSFTSAQILATVTESQVSVISDHTDKSLEMTELKLHEKDQSVPLPVYSGTPVQAESVVPSKICGLPLVEHASSVLPSIQENHLAVGDKSNPVYKIAERQVLKEDKPAFVNNYCPISDVFIDPLVNKHVPKEIHSEEKKDSVVQHCESILSIDDERDEMDKGHLKSNQSDTEMTNMEELTHIVSSVSDESTYPPLHLAASLKNSVPSTLIISEESKPLAQVQDSTSPTHLVTLLGSCSKCSPQEDKSKEDINSMTLSPCSTPLGRPLLDELPRFTNQRPSNLPSTTGTRSPFINWNSSSPVAQRVPHTSLRTEDHTQDITSYGLSPHKQSSSQAFNDKSSITPLGGGHKLDTKPPSAFSPSLRMRSPSPIRGTQSSRPTSTTSALTKSLAASCISQTISQSMAKRGAHIQTVSPPVRSSPLPTSSVRLRTPSPKPITLDSNVESGFRAISSPGVGSQPPRSCPSPFRSNSLRSSPATVQSPPPYRSQRSISPAPPVSLHSTPSVNSPTSTTLEQPSFSSLNCNNNNNNSLSNNGWVANNKKTAPSTVSGLPHFHDSQRTTSHNRVARPFPSSEPSSRVQSPSICLQSPITRICSPPPAPNHSSHLVTKPPNPRASRQGGYFTPLSLEISRSTSACSISPCGSPRITSPPPIGIPTNVWGYANPQPRNPLVTSASSHTKLETNSTPRGSRIISPTPTGSSSYSANSQSQRRLRGTSLPFVVIGDSLPSPTRHERRSWAESGRWSVGSDLGLISPREGSYSGSPSSVSPGPLSPVRLTAERTSGKHFTGVSWPDVHDLLIKYTEPNTEANALSCTEIEPGDIDGTEPTCRSTLICPYVAPASPVERDTPIQCQTEAKTEDCVPNQGSKTTLKTSYATTVNLQIAGSGRIASFSNAQVSLTQTLTPVADSQSRRRVSINSCNFSMQNCKRL

Target ORF information:

RefSeq Version	XM_021472942.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (plekhg2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472942.1

ORF Insert Sequence:

ATGCCTGAAG GTGCGGGACG CAACAGCCAG AAAAAAAACA GCAACCAAGC TGCCAAGCGC 
CCCTCCTCTG TGTCCTCGCT GAGTGGGATT GTGAGCCGCA TGGCCTCCTC AGGAGGCGCA 
TCCCGCGGGA GCTGCACCTC CGTTAACACA GTGTGCTCTG ACAGTGACCG TGCTGCTAGT 
CTAAGCTCCT CTGCCTCCTC TGCCTCGCTG CAGGATGGAC ACTCTTCGTC TTCGTCCTCA 
TCGCTCCCCT ACGGAGCAGT GCCAGCCTAC CCTGGGCCAC AGCATAATGG TTCTGATATC 
AGCCTGGACC TCACCCCTCT CTCCCTGCTG GCGGGAGCCG GTGCCCTCTC CACTTCCACA 
ACAACACTGC CCAAACTTAC TCGCCTGGAG AGGGTGGCGT TAGAGATACT GGAGACCGAA 
CAGGCCTATG TGAGGGACCT CAAGAGCATT GTGGAGGACT ATCTTGGCTG CATTATTGAC 
TGTGGCGACT TGCCGCTCAA ACCCGAAGAG GTCAGCACCC TCTTCTGCAA CATCGAGGAC 
ATCTATGAGT TTAACAGTGA GCTTTTGGAA GATCTGGAGC GCAGTCCGCA TGCGGCAGCC 
ATAGCAGAGT GCTTTGTGGA GAGGAGTGAA GCTTTTGACA TTTACACCAT ATACTGCATG 
AATTACCCCA ACTCAGTGAC CGTCCTGCGG GACTGCATGA AGAACGAGAG CCTGGTGCGC 
TTCTTCCAGG AAAGACAGGC CACCTTGTGT CACTCGCTGC CTCTGGAGAC CTACCTGCTA 
AAACCAGTGC AGAGAATCCT TAAATACCAC CTCCTGCTGC AGGAGCTGTC CAAACACTTT 
GATAAAAGTG ACCCTGGCTA TGAGGTGGTG GAAGATGCCA TTATAACTAT GACAGCAGTG 
GCCTGGTACA TCAATGACAT GAAGAGGAAG CAGGAACATG CAATCAGACT GCAGGAGATT 
GAGAGCTTGC TGCTGAACTG GACGGGTCCT GACCTCAGTG GTTTTGGAGA GCTGGTTCTT 
GAAGGTTCCT TCCGGGTTCA ACGCGTGAAA AAGGAAAGAG CTTTCTTTCT TTTTGACAAA 
ATGCTTCTTA TTGCCAAGAA GAGGCTAGAT CATTTCATAT ACAGTACTCA CATATTTTGC 
TGTAATCTGC TCTTAGTGGA AAATATGAAG GATCCCCTCT GTTTTAAGGT GTCTGACCAA 
ACAATCCCAA AACAACAACA TGTTGTTCAG GCTAAAAACC AGGAGGAGAA ACGACTGTGG 
CTACATTACC TCAAGAGATT GATCGTAGAA AACCATCCTG CATCCTTGCC TCAAAAAGCG 
AGGCAGGTTC TTGGTGACAA CTTCTGTCAG TCTCCACAGT TTGATCAGGA ACCAATTAGA 
AAGCCAATGC CGTCACCACG ACTGGATGAT GTTCATAACT ACCACAGAGG AAGAAGGCAA 
TCAGAGCCAC CAGAATTCAT CTACACCCCT GAAAAAGCCA AGAAAAGCCT TCCCATGCTG 
TTAGAGGGTA ACCTGCCATA CAAACGTGGA AGAAGACAGT CCGCGCCTGC CAAAGATATA 
GAAGCTGCAT TTCAGCAAAG CGGTTCTTTA AAGAGTTCTC AAGCTGGCAG TGAAGGGGAA 
TTGTGCCCAC AGGCTGATAG TATGGGCTCT TCAGGCAGTA CTAGTACTCT GGCATCCTCA 
GTCATTGAAG TTGAATCTGG ACGTGATGAT CTCGCCTTGT GTCAAGATGA AGATGATATG 
ACACCACTCC CTCCACCACC AACACTGTCT ATTACTGAAG AAATCATGGA GTTCATTAAC 
CAGAGCCGTG CTCGGCAGGG CATGACAGAG TTGATATCTG ATAGGTGTCA GTTGATTCCA 
GAGACCTTAG AAGTTCAGCC ATTGGAAGCA CAGGAGTCAG AGGAATCCAA ACCACTTATA 
GCTGAGATGG ATGAAAATAC ACATGCGATC ATTAATGTCC ACAGATCTGA TGCAGAGAAA 
ACTGAATCAG TCCAAGACGA GGACAGCCAT CCTTCCTGTG AAGAAAATAT TTCTCAACCA 
AATGAGCCGA AGGGAGTATC ACCAACTCTG CATGTCTCTA CTGAGGAAAA TCAATTAGAA 
GAGCTGACAT TCCAAGAGGC CAGTTGTGGT GAATCATGCA AATCAGAGAC CTTAAGTGAA 
GGAGAGAAAG ATAATGAGAA CTCTGTTAGT AAAAGTGAAA ATAAATCAGA TAAATCTGCA 
AATGAGACAG CATGTGAGGC CAATTCACAT TGTCCTCTTT TTGATACTTC AGAGAAAGCA 
TCTGAAAGCT CTCCCAAAAT AGAACCTGAG CAAAAACTGA CCAAAAGTGA CAAGCAGATT 
ATTGAGAAGA TTCGCAGCTA TTATGAGGCA GCGGAGACAG GAGTTGAGGA AGGTCAGTCT 
ACACGAAGAA ACAGCTTTTC TCATATTCCT GCAGGATTAG TGAAGGATTC TGTAACACGA 
TTCAATGTTT GTGTTCGTCA GGACAGTTTG TTTGAGTCTG AGAGTGGCCG CTCAGACACT 
GTTGACACTG ACTCAGCATT TTTAATACTC CCTGTATCTG ACCAAGCTGA CCAGACTTTC 
AGCCATCAGG AATCTGTTGA TGCAGACATT CCTCCCTCAC ATTCAGATGC TGACCATCAA 
GTGGAAGAAA TTGACGGCAA GACCTCAATG AAAAAGGATG AAGATGTTTG TGATTTCAGT 
ATGAAACTGT GGAAGGAGAA TGAAAGGACT GGCCAAGAGT CTCCAAGCAG TCCGAAAGTC 
CCCATCTCAA GAGAGAACAG CTGTGGTGAA CATACAGATG CTCCTGAGAA ATTGTCCGTA 
AATTGCATAA CAACCCAAGT ACAGCATAAA CAATCGGAGA GTTTACAATG TGATCCAGAC 
GCACCAGACA AATCTAATCT GCAGGTCACA GAGACAGCAC ATGCATCACT TCAATGTGCT 
AGTAAAACTA GACCTAGGAT GTCTTCTAAT GGAAGCCTGG ATAGCCTTCC CAGTCAGATT 
AAAGTTGGCA GATGGTCTCG TCATGGCAAG GTGGTTACAT GTAGCCAAAC CCTTTATGAA 
GGAATGGCCG AGGTACCAGA TTTAGGATTT TTTGAGGGTG GACCAGTCAA TCAGTGCTTG 
GTGGAAAACT CAGAAAAGAT TTTAAATAAA GTGCAGATGC TTGCACGCAT GTACACTGCC 
AAGGCAAGCA GCATGAAGGT ACCGCTACAT CAGAAAAAAA CACGAGTGAG TAGGGGAGCA 
TGGTCAGTGG ATGGAAAAGG GAGCACTTCA CCCAAACCCC AGCAAGTAAA GCTTCACCAG 
GGGGATATGA GGATAAGCCA GTCTGCAGAA GAATCTTTGG AAACTACACC TACTGAGCCT 
TTTGGTCATA TAATTTTAAG GGAGAAGCTA TCTACAACTT ATCACCAAGA AAATGATTGC 
AATCTTATTG GCCCGCTAGA TGAGATTTCA GAAACTGGAT CTAATTCACT GTCTTTTTCT 
TCTGCTGATT CCTCTTTCAC TTCGGCTCAA ATACTAGCAA CTGTTACAGA ATCACAGGTC 
TCTGTGATAT CAGACCATAC AGACAAGTCA TTAGAAATGA CAGAGTTGAA ACTACATGAA 
AAAGATCAAT CCGTACCACT TCCAGTATAC TCTGGAACAC CAGTCCAAGC TGAGTCAGTA 
GTCCCATCCA AAATCTGTGG GTTGCCATTG GTTGAGCATG CTAGCAGTGT ACTACCGTCC 
ATTCAGGAGA ACCATTTAGC TGTTGGGGAC AAATCAAATC CAGTATATAA AATTGCAGAA 
AGACAGGTAT TAAAAGAGGA TAAACCTGCT TTTGTGAACA ATTATTGTCC AATTAGTGAT 
GTTTTTATTG ACCCACTGGT CAATAAACAT GTACCTAAAG AGATTCACAG TGAGGAGAAA 
AAAGACTCTG TTGTACAGCA CTGTGAAAGT ATATTGTCTA TAGATGATGA ACGAGATGAA 
ATGGATAAAG GACATTTGAA ATCCAACCAG TCTGATACTG AAATGACTAA CATGGAGGAA 
CTAACACATA TAGTGTCCTC CGTTAGTGAT GAAAGCACAT ATCCTCCATT ACACTTAGCA 
GCCTCCTTAA AAAATAGTGT TCCATCCACT TTGATAATCT CTGAGGAATC AAAACCTTTG 
GCCCAGGTGC AGGATTCAAC TAGCCCTACG CATCTAGTGA CTCTGCTTGG TTCATGTAGT 
AAGTGTAGTC CACAGGAAGA TAAATCTAAA GAAGACATTA ATTCAATGAC TCTGAGCCCA 
TGCTCAACCC CACTTGGTAG ACCTCTTTTA GATGAGCTAC CAAGGTTTAC AAATCAAAGA 
CCCTCCAATC TGCCTTCCAC CACAGGAACG AGAAGTCCCT TCATAAATTG GAATTCATCG 
TCTCCAGTTG CTCAAAGAGT GCCACACACT TCACTGCGAA CAGAGGATCA CACTCAAGAT 
ATTACTTCTT ATGGCCTTTC TCCTCACAAG CAGTCTTCAT CCCAGGCTTT TAATGATAAG 
TCTTCAATTA CTCCCTTGGG TGGTGGACAT AAATTGGATA CAAAACCACC CTCTGCCTTT 
AGCCCAAGTC TCCGAATGAG GTCACCCTCT CCAATTAGAG GTACACAGAG CTCCAGACCT 
ACTTCAACAA CCTCAGCTCT TACAAAGTCT CTTGCTGCCT CCTGCATAAG TCAGACGATT 
TCTCAAAGCA TGGCCAAGAG AGGTGCACAT ATTCAGACTG TGTCCCCACC TGTAAGATCC 
TCTCCTCTAC CCACATCTTC CGTAAGATTA AGGACGCCAT CACCAAAACC CATCACCTTG 
GACTCAAATG TAGAGTCAGG ATTTAGGGCA ATATCCAGCC CTGGGGTAGG AAGTCAACCC 
CCAAGGTCTT GTCCATCCCC ATTCAGATCC AACAGTCTTC GATCTAGTCC AGCAACAGTT 
CAGTCCCCTC CTCCTTATCG TAGCCAAAGG TCAATATCTC CAGCACCTCC AGTTAGCCTC 
CATTCAACTC CATCTGTTAA CAGCCCAACC TCCACAACTC TTGAACAACC TTCGTTCAGC 
AGTTTAAATT GTAATAATAA CAATAATAAC AGCTTAAGTA ATAATGGGTG GGTTGCTAAT 
AACAAAAAAA CAGCACCATC AACTGTCAGT GGACTCCCTC ATTTCCATGA TTCCCAACGA 
ACAACCTCAC ACAATAGGGT GGCTCGTCCT TTCCCATCGT CTGAGCCTAG TTCCCGTGTA 
CAGTCCCCTT CCATTTGCCT CCAATCTCCA ATCACCCGAA TCTGCTCTCC TCCACCTGCT 
CCCAACCATT CCAGTCATCT GGTAACAAAG CCACCCAACC CCAGAGCATC CAGACAAGGT 
GGCTACTTTA CACCTTTATC TTTGGAAATT TCAAGATCAA CATCAGCTTG TTCAATATCT 
CCTTGCGGGA GTCCCAGAAT CACCTCTCCT CCCCCTATTG GCATTCCTAC AAATGTTTGG 
GGCTATGCTA ATCCACAACC AAGGAATCCA TTAGTAACAA GTGCATCGTC TCATACAAAA 
TTGGAAACAA ACTCTACACC AAGAGGCTCT AGAATCATCT CGCCCACCCC AACAGGATCA 
TCATCCTATT CAGCCAACTC TCAGAGTCAG AGGAGGTTAC GAGGAACCAG TTTGCCCTTT 
GTTGTGATAG GGGATAGCCT TCCAAGTCCA ACTAGACACG AACGCAGGTC TTGGGCTGAA 
AGTGGACGGT GGTCAGTGGG TTCAGATCTG GGTTTAATAA GTCCTCGTGA GGGTTCATAC 
AGCGGTAGCC CGTCTTCTGT TAGCCCTGGT CCTCTTTCAC CAGTCAGACT AACAGCCGAA 
AGGACAAGTG GAAAACACTT TACAGGCGTT TCCTGGCCAG ATGTACACGA TCTGTTGATT 
AAATACACAG AGCCCAATAC TGAAGCAAAT GCTTTATCCT GCACCGAGAT AGAGCCAGGT 
GACATAGACG GTACAGAACC TACATGTCGG AGTACCCTTA TTTGTCCATA TGTTGCTCCA 
GCCAGCCCTG TTGAGAGAGA CACTCCCATC CAGTGCCAAA CCGAGGCTAA AACAGAAGAC 
TGTGTTCCTA ATCAAGGGTC TAAAACAACA TTAAAGACCA GTTATGCCAC CACTGTCAAT 
TTGCAGATTG CTGGGAGTGG GAGGATTGCA TCCTTTAGCA ATGCGCAAGT CAGCCTGACT 
CAAACTCTGA CCCCTGTGGC AGACAGTCAG AGCAGGAGAA GAGTCAGCAT CAATAGCTGC 
AACTTCTCTA TGCAGAACTG CAAAAGGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHG2 ( XM_689253.9 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_694345.6 Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (plekhg2), mRNA.

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CloneID	ODa20412
Clone ID Related Accession (Same CDS sequence)	XM_689253.9 , XM_689253.8
Accession Version	XM_689253.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family G member 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_689253.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAAG GTGCGGGACG CAACAGCCAG AAAAAAAACA GCAACCAAGC TGCCAAGCGC 
CCCTCCTCTG TGTCCTCGCT GAGTGGGATT GTGAGCCGCA TGGCCTCCTC AGGAGGCGCA 
TCCCGCGGGA GCTGCACCTC CGTTAACACA GTGTGCTCTG ACAGTGACCG TGCTGCTAGT 
CTAAGCTCCT CTGCCTCCTC TGCCTCGCTG CAGGATGGAC ACTCTTCGTC TTCGTCCTCA 
TCGCTCCCCT ACGGAGCAGT GCCAGCCTAC CCTGGGCCAC AGCATAATGG TTCTGATATC 
AGCCTGGACC TCACCCCTCT CTCCCTGCTG GCGGGAGCCG GTGCCCTCTC CACTTCCACA 
ACAACACTGC CCAAACTTAC TCGCCTGGAG AGGGTGGCGT TAGAGATACT GGAGACCGAA 
CAGGCCTATG TGAGGGACCT CAAGAGCATT GTGGAGGACT ATCTTGGCTG CATTATTGAC 
TGTGGCGACT TGCCGCTCAA ACCCGAAGAG GTCAGCACCC TCTTCTGCAA CATCGAGGAC 
ATCTATGAGT TTAACAGTGA GCTTTTGGAA GATCTGGAGC GCAGTCCGCA TGCGGCAGCC 
ATAGCAGAGT GCTTTGTGGA GAGGAGTGAA GCTTTTGACA TTTACACCAT ATACTGCATG 
AATTACCCCA ACTCAGTGAC CGTCCTGCGG GACTGCATGA AGAACGAGAG CCTGGTGCGC 
TTCTTCCAGG AAAGACAGGC CACCTTGTGT CACTCGCTGC CTCTGGAGAC CTACCTGCTA 
AAACCAGTGC AGAGAATCCT TAAATACCAC CTCCTGCTGC AGGAGCTGTC CAAACACTTT 
GATAAAAGTG ACCCTGGCTA TGAGGTGGTG GAAGATGCCA TTATAACTAT GACAGCAGTG 
GCCTGGTACA TCAATGACAT GAAGAGGAAG CAGGAACATG CAATCAGATT GCAGGAGATT 
GAGAGCTTGC TGCTGAACTG GACGGGTCCT GACCTCAGTG GTTTTGGAGA GCTGGTTCTT 
GAAGGTTCCT TCCGGGTTCA ACGCGTGAAA AAGGAAAGAG CTTTCTTTCT TTTTGACAAA 
ATGCTTCTTA TTGCCAAGAA GAGGCTGGAT CATTTCATAT ACAGTACTCA CATATTTTGC 
TGTAATCTGC TCTTAGTGGA AAATATGAAG GATCCCCTCT GTTTTAAGGT GTCTGACCAA 
ACAATCCCAA AACAACAACA TGTTGTTCAG GCTAAAAACC AGGAGGAGAA GCGACTGTGG 
CTACATTACC TCAAGAGATT GATCGTAGAA AACCATCCTG CATCCTTGCC TCAAAAAGCG 
AGGCAGGTTC TTGGTGACAA CTTCTGTCAG TCTCCACAGT TTGATCAGGA ACCAATTAGA 
AAGCCAATGC CGTCACCACG ACTGGATGAT GTTCATAACT ACCACAGAGG AAGAAGGCAA 
TCAGAGCCAC CAGAATTCAT CTACACCCCT GAAAAAGCCA AGAAAAGCCT TCCCATGCTG 
TTAGAGGGTA ACCTGCCATA CAAACGTGGA AGAAGACAAT CTGCGCCTGC CAAAGATATA 
GAAGCTGCAT TTCAGCAAAG CGGTTCTTTA AAGAGTTCTC AAGCTGGCAG TGAAGGAGAA 
TTGTGCCCAC AGGCTGATAG TATGGGCTCT TCAGGCAGTA CCAGTACTCT GGCTTCCTCA 
GTCATTGAAG TTGAATCTGG ACGTGATGAT CTGGCCTTAT GTCAAGATGA AGATGATATG 
ACACCACTCC CTCCACCACC AACACTGTCT ATTACTGAAG AAATCATGGA ATTCATTAAC 
CAGAGCCGTG CTCGGCAGGG CATGACAGAG TTGACATCTG ATAGGTGTCA GTTGATTCCA 
GAGACCTTAG AAGTTCAGCC ATTGGAAGGA CAGGAGTCAG AGGAATCCAA ACCACTTATA 
GCTGAGATGG ATGAAAATAC ACATGCGATC ATTAATGTCC AAAGATCCGA TGCAGAGAAA 
ACTGAATCAG TTCAAGACGA GGACAGCCAT CCATCCTGTG AAGAAAATAT TTCTCAACCA 
AATGAGCCAA AGGGAGTATC ACCAACTCTG CATGTCTCTA CTGAGGAAAA TCAATTAGAA 
GAGCTGACAT TCCAAGAGGC CAGTTGTGGT GAATCATGCA AATCAGAGAC CTTAAGTGAA 
GGAGAGAAAG ATAATGAGAA CTCTGTTAGT AAAAGTGAAA ATAAATCAGC TAAATCTGCA 
AATGAGACAG CATGTGAGGC CAATTCACAT TGTCCTCTTA TTGATACTTC AGAGAAAGAA 
TCTGAAAGCT CTCCCAAAAT AGAACCTGAG CAAAAACTGA CCAAAAGTGA CAAGCAGATT 
ATTGAGAAGA TTCGCAGCTA TTATGAGGCA GCGGAGACAG GAGTTGAGGA AGGTCAGTCT 
ACACGAAGAA ACAGCTTTTC TCATATTCCT GCAGGATTAG TGAAGGATTC TGTAACACGA 
TTCAATGTTT GTGTTCGTCA GGACAGTTTG TTTGAGTCTG AGAGTGGCCG CTCAGACACT 
GTTGACACTG ACTCAGCATT TTTAATACTC CCTGTATCTG ACCAAGCTGA CCAGACTTTC 
AGCCATCAGG AATCTGTTGA TGCAGACATT CCTCCCTCAC ATTCAGATGC TGACCATCAA 
GTGGAAGAAA TTGACGGCAA GACCTCAATG AAAAAGGATG AAGATGTTTG TGATTTCAGT 
ATGAAACTGT GGAAGGAGAA TGAAAAGACT GGCCAAGAGT CTCCAAGCAG TCCGAAAGTC 
CCCATCTCAA GAGAGAACAG CTGTGGTGAA CATACAGATG CTCCTGAGAA ATTGTCCGTA 
AATTGCATAA CAACCCAAGT ACAGCATAAA CAATCGGAGA GTTTACAATG TGATCCAGAC 
GCACCAGACA AATCTAATCT GCAGGCCACA GAGACAGCAG ATGCATCACT TCAATGTGCA 
AGTAAAACTA GATCTAGGAT GTCTTCTAAT GTAAGCCTGG ATAGCCTTCC CATTCAGATT 
AAAGTTGGCA GATGGTCTCG TCATGGCAAG GTGGTTACAT GTAGCCAAAC CCTTTATGAA 
GGAATGGCCG AGGTACCAGA TTTAGGATTT TTTGAGAGTG GACCAGTCAA TCAGTGCTTG 
GTGGAAAACT CAGAAAAGAT TTTAAATAAA GTGCAAATGC TTGCACGCAT GTACACTGCC 
AAGGCGAGCA GCATGAAGGT ACCGCTGCAT CAGAAAAAAA CACGAGTGAG TAGGGGAGCA 
TGGTCAATGG ATGGAAAAGG GAGCACTTCA CCTAAACCTC AGCAAGTAAA GCTGCACCAG 
GGGGATATGA GGATAAGCCA GTCTGAAGAA GAATCTTTGG AAACTACACC TACTGAGCCT 
TTTGGTCATA TAATTTTAAG GGAGAAGCTG TCTACAACTT ATCACCAAGA AAATGATTGC 
AATCTTATTG GCCCGCTAGA TGAGATTTCA GAAACTGGAT CTAATTCACT GTCTTTTTCT 
TCTGCTGATT CCTCTTTCAC TTCGGCTCAA ATACTAGCAA CTGTTACAGA ATCAAAGGTC 
TCTGTGATAT CAGACCATAC AGACAGGTCA TTAGAAATGA CAGAGTCGAA ACTACATGAA 
AAAGAACAAT CCGTACCACT TCCAGTTTAC TCTGGAACAC CAGTCCAAGC TGAGTCAGTA 
GTCCCATCCA AAATCTGTGG GTTGCCATTG GTTGAGCATG CTAGCAGTGT ACTACAGTCC 
ATTCAGGAGA ACCATTTAGA TGTTGGGGAC AAATTAAATA CAGTATATAG AATTGCAGAA 
AGACAGGTAT TGCAAGAGGA TAAACCTGCT TTTGTAAACA ATTATTGTCC AATTAGTGAT 
GTTTTTATTG ACCCACTAGT CAATAAACAT GTAACTAAAG AGACTCACAG TGAGGAGAAA 
AAAGACTCTG TTGTACAGCA CTGTGAAAGT ATATTGTCTA TAGATGATGA ACGAGATGAA 
ATGGATAAAG GACATTTGAA ATCCAACCAG TCTGATACTG AAATGACTAA CATGGAGGAA 
CTAACACATA TAGTGTCCTC CGTTAGTGAT GAAAGCACAT ATCCTCCATT ACACTTAGCA 
GCCTCCTTAA AAAACAGTGT TCCATCCACT TTGATAATCT CTGAGGAATC AAAACCTTTG 
GCCCAGGTGC AGGATTTAAC TAGCCCTACG CATCTGGTGA CTCTGCTTGG TTCATGTAGC 
AAGTGTAGTC CACAGGAAGA TAAATCTAAA GAAGACATTA ATTCAATGAC TTTGAGCCCA 
TGCTCAACCC CACTTGGTAG ACCTCTTTTA GATGAGCTAC CAAGGTTTAC AAATCAAAGA 
CCCTCCAATC TGCCTTCCAC CACAGGAACG AGAAGTCCCT TCAAAAATTG GAATTCATCA 
TCTCCAGTTG CTCAAAGAGT GCCACACACT TCACTGCGAA CAGAGGATCA CACTCAAGAT 
ATTACTTCTT ATGGCCTTTC TCCTCACAAG CAGTCTTCAT CCCAGGCTTT TAATGATAAG 
TCTTCAATCA CTCCCTTGGG TGGTGAACAT AAATTAGATA CAAAACCACC CTCTGCCTTT 
AGTCCAAGTC TCCGAATGAG GTCACCCTCT CCAATTAGAG GTACACAGAG CTCCAGACCT 
ACTTCAACAA CCTCAGCTCT TACAAAGTCT CTTGCTGCCT CCTGCATCAG TCAGACGATT 
TCTCAAAGCA TGGCCAAGAG AGGTGCACAT ATTCAGACTG TGTCCCAACC TGTAAGATCC 
TCTCCTCTAC CCACATCTTC CGTAAGATTA CGGACGCCAT CACCAAAACC CATCACCTTG 
GACTCAAATG TAGAGTCAGG ATTTAGGGCA ATATCCAGCC CTGGGGTAGG AAGTCAACCC 
CCAAGGTCTT GTCCATCCCC ATTCAGATCC AACAGTCTTC GATCTAGTCC AGCAACAGTC 
CAGTCCCCTC CTCCTTATCG TAGCCAAAGG TCAATATCTC CAGCACCTCC AGTTAGCCTC 
CATTCAACTC CATCTGTTAA CAGCCCAACC TCCACTGCTC TTGAACAACC TTCGTTCAGC 
AGTTTAAATT GTAATAATAA CAATAATAAC AGCTTAAGTA ATAATGGGTG GGTCGCTAAT 
AACAAAAAAA CAGCACCATC AGCTGTAAGT GGACTCCCTC ATTTCCATGA TTCCCAGCGA 
ACAACCTCAC ACAATAGGGT GGCTCGTCCT TTCCCATCGT CTGAGCCTAG TTCCCGTGTA 
CAGTCCCCTT CCATTTGCCT CCAATCTCCA ATCACCCGAA TCTGCTCTCC TCCACCTGCT 
CCCAACCATT CCAGTCATCT GGTAACAAAG CCACCCAACC CCAGAGCATC CAGACAAGGT 
GGCTACTTTA CACCTTTATC TTTGGAAATT TCAAGATCAA CATCAGCTTG TTCAATATCT 
CCTTGCGGGA GTCCCAGAAT CACCTCTCCT CCCCCTATTG GCATTCCTAC AAATGTTTGG 
GGCTATGCTA ATCCACAACC AAGGAATCCA TTAGTAACAA GTGCATCGTC TCATACAAAA 
TTGGAAACAA ACTCTACTCC AAGAGGCTCT AGAATCATCT CGCCCACCCC AACAGGATCA 
TCATCCTATT CAGCCAACTC TCAGAGTCAG AGGAGGTTAC GAGGAACCAG TTTGCCCTTT 
GTTGTGATAG GGGATAGCCT TCCAAGTCCA ACTAGACACG AACGCAGATC TTGGGCTGAA 
AGTGGACGGT GGTCAGTGGG TTCAGATCTA GGTTTAATAA GTCCTCGTGG GGGTTCATAC 
AGTGGTAGCC CGTCTTCTGT TAGCCCTGGT CCTCTTTCAC CAGTCAGACT AACAGCCGAA 
AGGACAAGTG GAAAACACTT TACAGGCATT TCCTGGCCAG ATGTACACGA TCTCTTGATT 
AAATACACAG AGCCCAATAC TGAAGCAAAT GCTTTATCCT GCACCGAGAT AGAGCCAGGT 
GACATGGACG GTACAGAACC TACATGTCGG AGTACCCTTA TTTGTCCATA TGTTGCTCCA 
GCCAGCCCCA TTGAGAGAGA CACTCCCATT CAGTGCCAGA CCGAGGCTAA AACAGAAGAC 
TGTGTTCCTA ATCAAGGGTC TAAAACAACA TTAAAGACCA GTTATGCCAC CACTGTCAAT 
TTGCAGATTG CTGGGAGTGG GAGGATTGCA TCCTTTAGCA ATGCACAAGT CAGCCTGACT 
CAAACTCTGA CCCCTGTGGC AGACAGTCAG AGCAGGAGAA GAGTCAGCAT CAACAGCTGC 
AACTTCTCTA TGCAGAACTG CAAAAGGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_694345.6
CDS	557..6829
Translation	MPEGAGRNSQKKNSNQAAKRPSSVSSLSGIVSRMASSGGASRGSCTSVNTVCSDSDRAASLSSSASSASLQDGHSSSSSSSLPYGAVPAYPGPQHNGSDISLDLTPLSLLAGAGALSTSTTTLPKLTRLERVALEILETEQAYVRDLKSIVEDYLGCIIDCGDLPLKPEEVSTLFCNIEDIYEFNSELLEDLERSPHAAAIAECFVERSEAFDIYTIYCMNYPNSVTVLRDCMKNESLVRFFQERQATLCHSLPLETYLLKPVQRILKYHLLLQELSKHFDKSDPGYEVVEDAIITMTAVAWYINDMKRKQEHAIRLQEIESLLLNWTGPDLSGFGELVLEGSFRVQRVKKERAFFLFDKMLLIAKKRLDHFIYSTHIFCCNLLLVENMKDPLCFKVSDQTIPKQQHVVQAKNQEEKRLWLHYLKRLIVENHPASLPQKARQVLGDNFCQSPQFDQEPIRKPMPSPRLDDVHNYHRGRRQSEPPEFIYTPEKAKKSLPMLLEGNLPYKRGRRQSAPAKDIEAAFQQSGSLKSSQAGSEGELCPQADSMGSSGSTSTLASSVIEVESGRDDLALCQDEDDMTPLPPPPTLSITEEIMEFINQSRARQGMTELTSDRCQLIPETLEVQPLEGQESEESKPLIAEMDENTHAIINVQRSDAEKTESVQDEDSHPSCEENISQPNEPKGVSPTLHVSTEENQLEELTFQEASCGESCKSETLSEGEKDNENSVSKSENKSAKSANETACEANSHCPLIDTSEKESESSPKIEPEQKLTKSDKQIIEKIRSYYEAAETGVEEGQSTRRNSFSHIPAGLVKDSVTRFNVCVRQDSLFESESGRSDTVDTDSAFLILPVSDQADQTFSHQESVDADIPPSHSDADHQVEEIDGKTSMKKDEDVCDFSMKLWKENEKTGQESPSSPKVPISRENSCGEHTDAPEKLSVNCITTQVQHKQSESLQCDPDAPDKSNLQATETADASLQCASKTRSRMSSNVSLDSLPIQIKVGRWSRHGKVVTCSQTLYEGMAEVPDLGFFESGPVNQCLVENSEKILNKVQMLARMYTAKASSMKVPLHQKKTRVSRGAWSMDGKGSTSPKPQQVKLHQGDMRISQSEEESLETTPTEPFGHIILREKLSTTYHQENDCNLIGPLDEISETGSNSLSFSSADSSFTSAQILATVTESKVSVISDHTDRSLEMTESKLHEKEQSVPLPVYSGTPVQAESVVPSKICGLPLVEHASSVLQSIQENHLDVGDKLNTVYRIAERQVLQEDKPAFVNNYCPISDVFIDPLVNKHVTKETHSEEKKDSVVQHCESILSIDDERDEMDKGHLKSNQSDTEMTNMEELTHIVSSVSDESTYPPLHLAASLKNSVPSTLIISEESKPLAQVQDLTSPTHLVTLLGSCSKCSPQEDKSKEDINSMTLSPCSTPLGRPLLDELPRFTNQRPSNLPSTTGTRSPFKNWNSSSPVAQRVPHTSLRTEDHTQDITSYGLSPHKQSSSQAFNDKSSITPLGGEHKLDTKPPSAFSPSLRMRSPSPIRGTQSSRPTSTTSALTKSLAASCISQTISQSMAKRGAHIQTVSQPVRSSPLPTSSVRLRTPSPKPITLDSNVESGFRAISSPGVGSQPPRSCPSPFRSNSLRSSPATVQSPPPYRSQRSISPAPPVSLHSTPSVNSPTSTALEQPSFSSLNCNNNNNNSLSNNGWVANNKKTAPSAVSGLPHFHDSQRTTSHNRVARPFPSSEPSSRVQSPSICLQSPITRICSPPPAPNHSSHLVTKPPNPRASRQGGYFTPLSLEISRSTSACSISPCGSPRITSPPPIGIPTNVWGYANPQPRNPLVTSASSHTKLETNSTPRGSRIISPTPTGSSSYSANSQSQRRLRGTSLPFVVIGDSLPSPTRHERRSWAESGRWSVGSDLGLISPRGGSYSGSPSSVSPGPLSPVRLTAERTSGKHFTGISWPDVHDLLIKYTEPNTEANALSCTEIEPGDMDGTEPTCRSTLICPYVAPASPIERDTPIQCQTEAKTEDCVPNQGSKTTLKTSYATTVNLQIAGSGRIASFSNAQVSLTQTLTPVADSQSRRRVSINSCNFSMQNCKRL

Target ORF information:

RefSeq Version	XM_689253.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (plekhg2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_689253.9

ORF Insert Sequence:

ATGCCTGAAG GTGCGGGACG CAACAGCCAG AAAAAAAACA GCAACCAAGC TGCCAAGCGC 
CCCTCCTCTG TGTCCTCGCT GAGTGGGATT GTGAGCCGCA TGGCCTCCTC AGGAGGCGCA 
TCCCGCGGGA GCTGCACCTC CGTTAACACA GTGTGCTCTG ACAGTGACCG TGCTGCTAGT 
CTAAGCTCCT CTGCCTCCTC TGCCTCGCTG CAGGATGGAC ACTCTTCGTC TTCGTCCTCA 
TCGCTCCCCT ACGGAGCAGT GCCAGCCTAC CCTGGGCCAC AGCATAATGG TTCTGATATC 
AGCCTGGACC TCACCCCTCT CTCCCTGCTG GCGGGAGCCG GTGCCCTCTC CACTTCCACA 
ACAACACTGC CCAAACTTAC TCGCCTGGAG AGGGTGGCGT TAGAGATACT GGAGACCGAA 
CAGGCCTATG TGAGGGACCT CAAGAGCATT GTGGAGGACT ATCTTGGCTG CATTATTGAC 
TGTGGCGACT TGCCGCTCAA ACCCGAAGAG GTCAGCACCC TCTTCTGCAA CATCGAGGAC 
ATCTATGAGT TTAACAGTGA GCTTTTGGAA GATCTGGAGC GCAGTCCGCA TGCGGCAGCC 
ATAGCAGAGT GCTTTGTGGA GAGGAGTGAA GCTTTTGACA TTTACACCAT ATACTGCATG 
AATTACCCCA ACTCAGTGAC CGTCCTGCGG GACTGCATGA AGAACGAGAG CCTGGTGCGC 
TTCTTCCAGG AAAGACAGGC CACCTTGTGT CACTCGCTGC CTCTGGAGAC CTACCTGCTA 
AAACCAGTGC AGAGAATCCT TAAATACCAC CTCCTGCTGC AGGAGCTGTC CAAACACTTT 
GATAAAAGTG ACCCTGGCTA TGAGGTGGTG GAAGATGCCA TTATAACTAT GACAGCAGTG 
GCCTGGTACA TCAATGACAT GAAGAGGAAG CAGGAACATG CAATCAGATT GCAGGAGATT 
GAGAGCTTGC TGCTGAACTG GACGGGTCCT GACCTCAGTG GTTTTGGAGA GCTGGTTCTT 
GAAGGTTCCT TCCGGGTTCA ACGCGTGAAA AAGGAAAGAG CTTTCTTTCT TTTTGACAAA 
ATGCTTCTTA TTGCCAAGAA GAGGCTGGAT CATTTCATAT ACAGTACTCA CATATTTTGC 
TGTAATCTGC TCTTAGTGGA AAATATGAAG GATCCCCTCT GTTTTAAGGT GTCTGACCAA 
ACAATCCCAA AACAACAACA TGTTGTTCAG GCTAAAAACC AGGAGGAGAA GCGACTGTGG 
CTACATTACC TCAAGAGATT GATCGTAGAA AACCATCCTG CATCCTTGCC TCAAAAAGCG 
AGGCAGGTTC TTGGTGACAA CTTCTGTCAG TCTCCACAGT TTGATCAGGA ACCAATTAGA 
AAGCCAATGC CGTCACCACG ACTGGATGAT GTTCATAACT ACCACAGAGG AAGAAGGCAA 
TCAGAGCCAC CAGAATTCAT CTACACCCCT GAAAAAGCCA AGAAAAGCCT TCCCATGCTG 
TTAGAGGGTA ACCTGCCATA CAAACGTGGA AGAAGACAAT CTGCGCCTGC CAAAGATATA 
GAAGCTGCAT TTCAGCAAAG CGGTTCTTTA AAGAGTTCTC AAGCTGGCAG TGAAGGAGAA 
TTGTGCCCAC AGGCTGATAG TATGGGCTCT TCAGGCAGTA CCAGTACTCT GGCTTCCTCA 
GTCATTGAAG TTGAATCTGG ACGTGATGAT CTGGCCTTAT GTCAAGATGA AGATGATATG 
ACACCACTCC CTCCACCACC AACACTGTCT ATTACTGAAG AAATCATGGA ATTCATTAAC 
CAGAGCCGTG CTCGGCAGGG CATGACAGAG TTGACATCTG ATAGGTGTCA GTTGATTCCA 
GAGACCTTAG AAGTTCAGCC ATTGGAAGGA CAGGAGTCAG AGGAATCCAA ACCACTTATA 
GCTGAGATGG ATGAAAATAC ACATGCGATC ATTAATGTCC AAAGATCCGA TGCAGAGAAA 
ACTGAATCAG TTCAAGACGA GGACAGCCAT CCATCCTGTG AAGAAAATAT TTCTCAACCA 
AATGAGCCAA AGGGAGTATC ACCAACTCTG CATGTCTCTA CTGAGGAAAA TCAATTAGAA 
GAGCTGACAT TCCAAGAGGC CAGTTGTGGT GAATCATGCA AATCAGAGAC CTTAAGTGAA 
GGAGAGAAAG ATAATGAGAA CTCTGTTAGT AAAAGTGAAA ATAAATCAGC TAAATCTGCA 
AATGAGACAG CATGTGAGGC CAATTCACAT TGTCCTCTTA TTGATACTTC AGAGAAAGAA 
TCTGAAAGCT CTCCCAAAAT AGAACCTGAG CAAAAACTGA CCAAAAGTGA CAAGCAGATT 
ATTGAGAAGA TTCGCAGCTA TTATGAGGCA GCGGAGACAG GAGTTGAGGA AGGTCAGTCT 
ACACGAAGAA ACAGCTTTTC TCATATTCCT GCAGGATTAG TGAAGGATTC TGTAACACGA 
TTCAATGTTT GTGTTCGTCA GGACAGTTTG TTTGAGTCTG AGAGTGGCCG CTCAGACACT 
GTTGACACTG ACTCAGCATT TTTAATACTC CCTGTATCTG ACCAAGCTGA CCAGACTTTC 
AGCCATCAGG AATCTGTTGA TGCAGACATT CCTCCCTCAC ATTCAGATGC TGACCATCAA 
GTGGAAGAAA TTGACGGCAA GACCTCAATG AAAAAGGATG AAGATGTTTG TGATTTCAGT 
ATGAAACTGT GGAAGGAGAA TGAAAAGACT GGCCAAGAGT CTCCAAGCAG TCCGAAAGTC 
CCCATCTCAA GAGAGAACAG CTGTGGTGAA CATACAGATG CTCCTGAGAA ATTGTCCGTA 
AATTGCATAA CAACCCAAGT ACAGCATAAA CAATCGGAGA GTTTACAATG TGATCCAGAC 
GCACCAGACA AATCTAATCT GCAGGCCACA GAGACAGCAG ATGCATCACT TCAATGTGCA 
AGTAAAACTA GATCTAGGAT GTCTTCTAAT GTAAGCCTGG ATAGCCTTCC CATTCAGATT 
AAAGTTGGCA GATGGTCTCG TCATGGCAAG GTGGTTACAT GTAGCCAAAC CCTTTATGAA 
GGAATGGCCG AGGTACCAGA TTTAGGATTT TTTGAGAGTG GACCAGTCAA TCAGTGCTTG 
GTGGAAAACT CAGAAAAGAT TTTAAATAAA GTGCAAATGC TTGCACGCAT GTACACTGCC 
AAGGCGAGCA GCATGAAGGT ACCGCTGCAT CAGAAAAAAA CACGAGTGAG TAGGGGAGCA 
TGGTCAATGG ATGGAAAAGG GAGCACTTCA CCTAAACCTC AGCAAGTAAA GCTGCACCAG 
GGGGATATGA GGATAAGCCA GTCTGAAGAA GAATCTTTGG AAACTACACC TACTGAGCCT 
TTTGGTCATA TAATTTTAAG GGAGAAGCTG TCTACAACTT ATCACCAAGA AAATGATTGC 
AATCTTATTG GCCCGCTAGA TGAGATTTCA GAAACTGGAT CTAATTCACT GTCTTTTTCT 
TCTGCTGATT CCTCTTTCAC TTCGGCTCAA ATACTAGCAA CTGTTACAGA ATCAAAGGTC 
TCTGTGATAT CAGACCATAC AGACAGGTCA TTAGAAATGA CAGAGTCGAA ACTACATGAA 
AAAGAACAAT CCGTACCACT TCCAGTTTAC TCTGGAACAC CAGTCCAAGC TGAGTCAGTA 
GTCCCATCCA AAATCTGTGG GTTGCCATTG GTTGAGCATG CTAGCAGTGT ACTACAGTCC 
ATTCAGGAGA ACCATTTAGA TGTTGGGGAC AAATTAAATA CAGTATATAG AATTGCAGAA 
AGACAGGTAT TGCAAGAGGA TAAACCTGCT TTTGTAAACA ATTATTGTCC AATTAGTGAT 
GTTTTTATTG ACCCACTAGT CAATAAACAT GTAACTAAAG AGACTCACAG TGAGGAGAAA 
AAAGACTCTG TTGTACAGCA CTGTGAAAGT ATATTGTCTA TAGATGATGA ACGAGATGAA 
ATGGATAAAG GACATTTGAA ATCCAACCAG TCTGATACTG AAATGACTAA CATGGAGGAA 
CTAACACATA TAGTGTCCTC CGTTAGTGAT GAAAGCACAT ATCCTCCATT ACACTTAGCA 
GCCTCCTTAA AAAACAGTGT TCCATCCACT TTGATAATCT CTGAGGAATC AAAACCTTTG 
GCCCAGGTGC AGGATTTAAC TAGCCCTACG CATCTGGTGA CTCTGCTTGG TTCATGTAGC 
AAGTGTAGTC CACAGGAAGA TAAATCTAAA GAAGACATTA ATTCAATGAC TTTGAGCCCA 
TGCTCAACCC CACTTGGTAG ACCTCTTTTA GATGAGCTAC CAAGGTTTAC AAATCAAAGA 
CCCTCCAATC TGCCTTCCAC CACAGGAACG AGAAGTCCCT TCAAAAATTG GAATTCATCA 
TCTCCAGTTG CTCAAAGAGT GCCACACACT TCACTGCGAA CAGAGGATCA CACTCAAGAT 
ATTACTTCTT ATGGCCTTTC TCCTCACAAG CAGTCTTCAT CCCAGGCTTT TAATGATAAG 
TCTTCAATCA CTCCCTTGGG TGGTGAACAT AAATTAGATA CAAAACCACC CTCTGCCTTT 
AGTCCAAGTC TCCGAATGAG GTCACCCTCT CCAATTAGAG GTACACAGAG CTCCAGACCT 
ACTTCAACAA CCTCAGCTCT TACAAAGTCT CTTGCTGCCT CCTGCATCAG TCAGACGATT 
TCTCAAAGCA TGGCCAAGAG AGGTGCACAT ATTCAGACTG TGTCCCAACC TGTAAGATCC 
TCTCCTCTAC CCACATCTTC CGTAAGATTA CGGACGCCAT CACCAAAACC CATCACCTTG 
GACTCAAATG TAGAGTCAGG ATTTAGGGCA ATATCCAGCC CTGGGGTAGG AAGTCAACCC 
CCAAGGTCTT GTCCATCCCC ATTCAGATCC AACAGTCTTC GATCTAGTCC AGCAACAGTC 
CAGTCCCCTC CTCCTTATCG TAGCCAAAGG TCAATATCTC CAGCACCTCC AGTTAGCCTC 
CATTCAACTC CATCTGTTAA CAGCCCAACC TCCACTGCTC TTGAACAACC TTCGTTCAGC 
AGTTTAAATT GTAATAATAA CAATAATAAC AGCTTAAGTA ATAATGGGTG GGTCGCTAAT 
AACAAAAAAA CAGCACCATC AGCTGTAAGT GGACTCCCTC ATTTCCATGA TTCCCAGCGA 
ACAACCTCAC ACAATAGGGT GGCTCGTCCT TTCCCATCGT CTGAGCCTAG TTCCCGTGTA 
CAGTCCCCTT CCATTTGCCT CCAATCTCCA ATCACCCGAA TCTGCTCTCC TCCACCTGCT 
CCCAACCATT CCAGTCATCT GGTAACAAAG CCACCCAACC CCAGAGCATC CAGACAAGGT 
GGCTACTTTA CACCTTTATC TTTGGAAATT TCAAGATCAA CATCAGCTTG TTCAATATCT 
CCTTGCGGGA GTCCCAGAAT CACCTCTCCT CCCCCTATTG GCATTCCTAC AAATGTTTGG 
GGCTATGCTA ATCCACAACC AAGGAATCCA TTAGTAACAA GTGCATCGTC TCATACAAAA 
TTGGAAACAA ACTCTACTCC AAGAGGCTCT AGAATCATCT CGCCCACCCC AACAGGATCA 
TCATCCTATT CAGCCAACTC TCAGAGTCAG AGGAGGTTAC GAGGAACCAG TTTGCCCTTT 
GTTGTGATAG GGGATAGCCT TCCAAGTCCA ACTAGACACG AACGCAGATC TTGGGCTGAA 
AGTGGACGGT GGTCAGTGGG TTCAGATCTA GGTTTAATAA GTCCTCGTGG GGGTTCATAC 
AGTGGTAGCC CGTCTTCTGT TAGCCCTGGT CCTCTTTCAC CAGTCAGACT AACAGCCGAA 
AGGACAAGTG GAAAACACTT TACAGGCATT TCCTGGCCAG ATGTACACGA TCTCTTGATT 
AAATACACAG AGCCCAATAC TGAAGCAAAT GCTTTATCCT GCACCGAGAT AGAGCCAGGT 
GACATGGACG GTACAGAACC TACATGTCGG AGTACCCTTA TTTGTCCATA TGTTGCTCCA 
GCCAGCCCCA TTGAGAGAGA CACTCCCATT CAGTGCCAGA CCGAGGCTAA AACAGAAGAC 
TGTGTTCCTA ATCAAGGGTC TAAAACAACA TTAAAGACCA GTTATGCCAC CACTGTCAAT 
TTGCAGATTG CTGGGAGTGG GAGGATTGCA TCCTTTAGCA ATGCACAAGT CAGCCTGACT 
CAAACTCTGA CCCCTGTGGC AGACAGTCAG AGCAGGAGAA GAGTCAGCAT CAACAGCTGC 
AACTTCTCTA TGCAGAACTG CAAAAGGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHG2 ( XM_689253.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_694345.6 Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (plekhg2), transcript variant X1, mRNA.

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CloneID	ODa20412
Clone ID Related Accession (Same CDS sequence)	XM_689253.9 , XM_689253.8
Accession Version	XM_689253.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family G member 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_689253.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAAG GTGCGGGACG CAACAGCCAG AAAAAAAACA GCAACCAAGC TGCCAAGCGC 
CCCTCCTCTG TGTCCTCGCT GAGTGGGATT GTGAGCCGCA TGGCCTCCTC AGGAGGCGCA 
TCCCGCGGGA GCTGCACCTC CGTTAACACA GTGTGCTCTG ACAGTGACCG TGCTGCTAGT 
CTAAGCTCCT CTGCCTCCTC TGCCTCGCTG CAGGATGGAC ACTCTTCGTC TTCGTCCTCA 
TCGCTCCCCT ACGGAGCAGT GCCAGCCTAC CCTGGGCCAC AGCATAATGG TTCTGATATC 
AGCCTGGACC TCACCCCTCT CTCCCTGCTG GCGGGAGCCG GTGCCCTCTC CACTTCCACA 
ACAACACTGC CCAAACTTAC TCGCCTGGAG AGGGTGGCGT TAGAGATACT GGAGACCGAA 
CAGGCCTATG TGAGGGACCT CAAGAGCATT GTGGAGGACT ATCTTGGCTG CATTATTGAC 
TGTGGCGACT TGCCGCTCAA ACCCGAAGAG GTCAGCACCC TCTTCTGCAA CATCGAGGAC 
ATCTATGAGT TTAACAGTGA GCTTTTGGAA GATCTGGAGC GCAGTCCGCA TGCGGCAGCC 
ATAGCAGAGT GCTTTGTGGA GAGGAGTGAA GCTTTTGACA TTTACACCAT ATACTGCATG 
AATTACCCCA ACTCAGTGAC CGTCCTGCGG GACTGCATGA AGAACGAGAG CCTGGTGCGC 
TTCTTCCAGG AAAGACAGGC CACCTTGTGT CACTCGCTGC CTCTGGAGAC CTACCTGCTA 
AAACCAGTGC AGAGAATCCT TAAATACCAC CTCCTGCTGC AGGAGCTGTC CAAACACTTT 
GATAAAAGTG ACCCTGGCTA TGAGGTGGTG GAAGATGCCA TTATAACTAT GACAGCAGTG 
GCCTGGTACA TCAATGACAT GAAGAGGAAG CAGGAACATG CAATCAGATT GCAGGAGATT 
GAGAGCTTGC TGCTGAACTG GACGGGTCCT GACCTCAGTG GTTTTGGAGA GCTGGTTCTT 
GAAGGTTCCT TCCGGGTTCA ACGCGTGAAA AAGGAAAGAG CTTTCTTTCT TTTTGACAAA 
ATGCTTCTTA TTGCCAAGAA GAGGCTGGAT CATTTCATAT ACAGTACTCA CATATTTTGC 
TGTAATCTGC TCTTAGTGGA AAATATGAAG GATCCCCTCT GTTTTAAGGT GTCTGACCAA 
ACAATCCCAA AACAACAACA TGTTGTTCAG GCTAAAAACC AGGAGGAGAA GCGACTGTGG 
CTACATTACC TCAAGAGATT GATCGTAGAA AACCATCCTG CATCCTTGCC TCAAAAAGCG 
AGGCAGGTTC TTGGTGACAA CTTCTGTCAG TCTCCACAGT TTGATCAGGA ACCAATTAGA 
AAGCCAATGC CGTCACCACG ACTGGATGAT GTTCATAACT ACCACAGAGG AAGAAGGCAA 
TCAGAGCCAC CAGAATTCAT CTACACCCCT GAAAAAGCCA AGAAAAGCCT TCCCATGCTG 
TTAGAGGGTA ACCTGCCATA CAAACGTGGA AGAAGACAAT CTGCGCCTGC CAAAGATATA 
GAAGCTGCAT TTCAGCAAAG CGGTTCTTTA AAGAGTTCTC AAGCTGGCAG TGAAGGAGAA 
TTGTGCCCAC AGGCTGATAG TATGGGCTCT TCAGGCAGTA CCAGTACTCT GGCTTCCTCA 
GTCATTGAAG TTGAATCTGG ACGTGATGAT CTGGCCTTAT GTCAAGATGA AGATGATATG 
ACACCACTCC CTCCACCACC AACACTGTCT ATTACTGAAG AAATCATGGA ATTCATTAAC 
CAGAGCCGTG CTCGGCAGGG CATGACAGAG TTGACATCTG ATAGGTGTCA GTTGATTCCA 
GAGACCTTAG AAGTTCAGCC ATTGGAAGGA CAGGAGTCAG AGGAATCCAA ACCACTTATA 
GCTGAGATGG ATGAAAATAC ACATGCGATC ATTAATGTCC AAAGATCCGA TGCAGAGAAA 
ACTGAATCAG TTCAAGACGA GGACAGCCAT CCATCCTGTG AAGAAAATAT TTCTCAACCA 
AATGAGCCAA AGGGAGTATC ACCAACTCTG CATGTCTCTA CTGAGGAAAA TCAATTAGAA 
GAGCTGACAT TCCAAGAGGC CAGTTGTGGT GAATCATGCA AATCAGAGAC CTTAAGTGAA 
GGAGAGAAAG ATAATGAGAA CTCTGTTAGT AAAAGTGAAA ATAAATCAGC TAAATCTGCA 
AATGAGACAG CATGTGAGGC CAATTCACAT TGTCCTCTTA TTGATACTTC AGAGAAAGAA 
TCTGAAAGCT CTCCCAAAAT AGAACCTGAG CAAAAACTGA CCAAAAGTGA CAAGCAGATT 
ATTGAGAAGA TTCGCAGCTA TTATGAGGCA GCGGAGACAG GAGTTGAGGA AGGTCAGTCT 
ACACGAAGAA ACAGCTTTTC TCATATTCCT GCAGGATTAG TGAAGGATTC TGTAACACGA 
TTCAATGTTT GTGTTCGTCA GGACAGTTTG TTTGAGTCTG AGAGTGGCCG CTCAGACACT 
GTTGACACTG ACTCAGCATT TTTAATACTC CCTGTATCTG ACCAAGCTGA CCAGACTTTC 
AGCCATCAGG AATCTGTTGA TGCAGACATT CCTCCCTCAC ATTCAGATGC TGACCATCAA 
GTGGAAGAAA TTGACGGCAA GACCTCAATG AAAAAGGATG AAGATGTTTG TGATTTCAGT 
ATGAAACTGT GGAAGGAGAA TGAAAAGACT GGCCAAGAGT CTCCAAGCAG TCCGAAAGTC 
CCCATCTCAA GAGAGAACAG CTGTGGTGAA CATACAGATG CTCCTGAGAA ATTGTCCGTA 
AATTGCATAA CAACCCAAGT ACAGCATAAA CAATCGGAGA GTTTACAATG TGATCCAGAC 
GCACCAGACA AATCTAATCT GCAGGCCACA GAGACAGCAG ATGCATCACT TCAATGTGCA 
AGTAAAACTA GATCTAGGAT GTCTTCTAAT GTAAGCCTGG ATAGCCTTCC CATTCAGATT 
AAAGTTGGCA GATGGTCTCG TCATGGCAAG GTGGTTACAT GTAGCCAAAC CCTTTATGAA 
GGAATGGCCG AGGTACCAGA TTTAGGATTT TTTGAGAGTG GACCAGTCAA TCAGTGCTTG 
GTGGAAAACT CAGAAAAGAT TTTAAATAAA GTGCAAATGC TTGCACGCAT GTACACTGCC 
AAGGCGAGCA GCATGAAGGT ACCGCTGCAT CAGAAAAAAA CACGAGTGAG TAGGGGAGCA 
TGGTCAATGG ATGGAAAAGG GAGCACTTCA CCTAAACCTC AGCAAGTAAA GCTGCACCAG 
GGGGATATGA GGATAAGCCA GTCTGAAGAA GAATCTTTGG AAACTACACC TACTGAGCCT 
TTTGGTCATA TAATTTTAAG GGAGAAGCTG TCTACAACTT ATCACCAAGA AAATGATTGC 
AATCTTATTG GCCCGCTAGA TGAGATTTCA GAAACTGGAT CTAATTCACT GTCTTTTTCT 
TCTGCTGATT CCTCTTTCAC TTCGGCTCAA ATACTAGCAA CTGTTACAGA ATCAAAGGTC 
TCTGTGATAT CAGACCATAC AGACAGGTCA TTAGAAATGA CAGAGTCGAA ACTACATGAA 
AAAGAACAAT CCGTACCACT TCCAGTTTAC TCTGGAACAC CAGTCCAAGC TGAGTCAGTA 
GTCCCATCCA AAATCTGTGG GTTGCCATTG GTTGAGCATG CTAGCAGTGT ACTACAGTCC 
ATTCAGGAGA ACCATTTAGA TGTTGGGGAC AAATTAAATA CAGTATATAG AATTGCAGAA 
AGACAGGTAT TGCAAGAGGA TAAACCTGCT TTTGTAAACA ATTATTGTCC AATTAGTGAT 
GTTTTTATTG ACCCACTAGT CAATAAACAT GTAACTAAAG AGACTCACAG TGAGGAGAAA 
AAAGACTCTG TTGTACAGCA CTGTGAAAGT ATATTGTCTA TAGATGATGA ACGAGATGAA 
ATGGATAAAG GACATTTGAA ATCCAACCAG TCTGATACTG AAATGACTAA CATGGAGGAA 
CTAACACATA TAGTGTCCTC CGTTAGTGAT GAAAGCACAT ATCCTCCATT ACACTTAGCA 
GCCTCCTTAA AAAACAGTGT TCCATCCACT TTGATAATCT CTGAGGAATC AAAACCTTTG 
GCCCAGGTGC AGGATTTAAC TAGCCCTACG CATCTGGTGA CTCTGCTTGG TTCATGTAGC 
AAGTGTAGTC CACAGGAAGA TAAATCTAAA GAAGACATTA ATTCAATGAC TTTGAGCCCA 
TGCTCAACCC CACTTGGTAG ACCTCTTTTA GATGAGCTAC CAAGGTTTAC AAATCAAAGA 
CCCTCCAATC TGCCTTCCAC CACAGGAACG AGAAGTCCCT TCAAAAATTG GAATTCATCA 
TCTCCAGTTG CTCAAAGAGT GCCACACACT TCACTGCGAA CAGAGGATCA CACTCAAGAT 
ATTACTTCTT ATGGCCTTTC TCCTCACAAG CAGTCTTCAT CCCAGGCTTT TAATGATAAG 
TCTTCAATCA CTCCCTTGGG TGGTGAACAT AAATTAGATA CAAAACCACC CTCTGCCTTT 
AGTCCAAGTC TCCGAATGAG GTCACCCTCT CCAATTAGAG GTACACAGAG CTCCAGACCT 
ACTTCAACAA CCTCAGCTCT TACAAAGTCT CTTGCTGCCT CCTGCATCAG TCAGACGATT 
TCTCAAAGCA TGGCCAAGAG AGGTGCACAT ATTCAGACTG TGTCCCAACC TGTAAGATCC 
TCTCCTCTAC CCACATCTTC CGTAAGATTA CGGACGCCAT CACCAAAACC CATCACCTTG 
GACTCAAATG TAGAGTCAGG ATTTAGGGCA ATATCCAGCC CTGGGGTAGG AAGTCAACCC 
CCAAGGTCTT GTCCATCCCC ATTCAGATCC AACAGTCTTC GATCTAGTCC AGCAACAGTC 
CAGTCCCCTC CTCCTTATCG TAGCCAAAGG TCAATATCTC CAGCACCTCC AGTTAGCCTC 
CATTCAACTC CATCTGTTAA CAGCCCAACC TCCACTGCTC TTGAACAACC TTCGTTCAGC 
AGTTTAAATT GTAATAATAA CAATAATAAC AGCTTAAGTA ATAATGGGTG GGTCGCTAAT 
AACAAAAAAA CAGCACCATC AGCTGTAAGT GGACTCCCTC ATTTCCATGA TTCCCAGCGA 
ACAACCTCAC ACAATAGGGT GGCTCGTCCT TTCCCATCGT CTGAGCCTAG TTCCCGTGTA 
CAGTCCCCTT CCATTTGCCT CCAATCTCCA ATCACCCGAA TCTGCTCTCC TCCACCTGCT 
CCCAACCATT CCAGTCATCT GGTAACAAAG CCACCCAACC CCAGAGCATC CAGACAAGGT 
GGCTACTTTA CACCTTTATC TTTGGAAATT TCAAGATCAA CATCAGCTTG TTCAATATCT 
CCTTGCGGGA GTCCCAGAAT CACCTCTCCT CCCCCTATTG GCATTCCTAC AAATGTTTGG 
GGCTATGCTA ATCCACAACC AAGGAATCCA TTAGTAACAA GTGCATCGTC TCATACAAAA 
TTGGAAACAA ACTCTACTCC AAGAGGCTCT AGAATCATCT CGCCCACCCC AACAGGATCA 
TCATCCTATT CAGCCAACTC TCAGAGTCAG AGGAGGTTAC GAGGAACCAG TTTGCCCTTT 
GTTGTGATAG GGGATAGCCT TCCAAGTCCA ACTAGACACG AACGCAGATC TTGGGCTGAA 
AGTGGACGGT GGTCAGTGGG TTCAGATCTA GGTTTAATAA GTCCTCGTGG GGGTTCATAC 
AGTGGTAGCC CGTCTTCTGT TAGCCCTGGT CCTCTTTCAC CAGTCAGACT AACAGCCGAA 
AGGACAAGTG GAAAACACTT TACAGGCATT TCCTGGCCAG ATGTACACGA TCTCTTGATT 
AAATACACAG AGCCCAATAC TGAAGCAAAT GCTTTATCCT GCACCGAGAT AGAGCCAGGT 
GACATGGACG GTACAGAACC TACATGTCGG AGTACCCTTA TTTGTCCATA TGTTGCTCCA 
GCCAGCCCCA TTGAGAGAGA CACTCCCATT CAGTGCCAGA CCGAGGCTAA AACAGAAGAC 
TGTGTTCCTA ATCAAGGGTC TAAAACAACA TTAAAGACCA GTTATGCCAC CACTGTCAAT 
TTGCAGATTG CTGGGAGTGG GAGGATTGCA TCCTTTAGCA ATGCACAAGT CAGCCTGACT 
CAAACTCTGA CCCCTGTGGC AGACAGTCAG AGCAGGAGAA GAGTCAGCAT CAACAGCTGC 
AACTTCTCTA TGCAGAACTG CAAAAGGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_694345.6
CDS	545..6817
Translation	MPEGAGRNSQKKNSNQAAKRPSSVSSLSGIVSRMASSGGASRGSCTSVNTVCSDSDRAASLSSSASSASLQDGHSSSSSSSLPYGAVPAYPGPQHNGSDISLDLTPLSLLAGAGALSTSTTTLPKLTRLERVALEILETEQAYVRDLKSIVEDYLGCIIDCGDLPLKPEEVSTLFCNIEDIYEFNSELLEDLERSPHAAAIAECFVERSEAFDIYTIYCMNYPNSVTVLRDCMKNESLVRFFQERQATLCHSLPLETYLLKPVQRILKYHLLLQELSKHFDKSDPGYEVVEDAIITMTAVAWYINDMKRKQEHAIRLQEIESLLLNWTGPDLSGFGELVLEGSFRVQRVKKERAFFLFDKMLLIAKKRLDHFIYSTHIFCCNLLLVENMKDPLCFKVSDQTIPKQQHVVQAKNQEEKRLWLHYLKRLIVENHPASLPQKARQVLGDNFCQSPQFDQEPIRKPMPSPRLDDVHNYHRGRRQSEPPEFIYTPEKAKKSLPMLLEGNLPYKRGRRQSAPAKDIEAAFQQSGSLKSSQAGSEGELCPQADSMGSSGSTSTLASSVIEVESGRDDLALCQDEDDMTPLPPPPTLSITEEIMEFINQSRARQGMTELTSDRCQLIPETLEVQPLEGQESEESKPLIAEMDENTHAIINVQRSDAEKTESVQDEDSHPSCEENISQPNEPKGVSPTLHVSTEENQLEELTFQEASCGESCKSETLSEGEKDNENSVSKSENKSAKSANETACEANSHCPLIDTSEKESESSPKIEPEQKLTKSDKQIIEKIRSYYEAAETGVEEGQSTRRNSFSHIPAGLVKDSVTRFNVCVRQDSLFESESGRSDTVDTDSAFLILPVSDQADQTFSHQESVDADIPPSHSDADHQVEEIDGKTSMKKDEDVCDFSMKLWKENEKTGQESPSSPKVPISRENSCGEHTDAPEKLSVNCITTQVQHKQSESLQCDPDAPDKSNLQATETADASLQCASKTRSRMSSNVSLDSLPIQIKVGRWSRHGKVVTCSQTLYEGMAEVPDLGFFESGPVNQCLVENSEKILNKVQMLARMYTAKASSMKVPLHQKKTRVSRGAWSMDGKGSTSPKPQQVKLHQGDMRISQSEEESLETTPTEPFGHIILREKLSTTYHQENDCNLIGPLDEISETGSNSLSFSSADSSFTSAQILATVTESKVSVISDHTDRSLEMTESKLHEKEQSVPLPVYSGTPVQAESVVPSKICGLPLVEHASSVLQSIQENHLDVGDKLNTVYRIAERQVLQEDKPAFVNNYCPISDVFIDPLVNKHVTKETHSEEKKDSVVQHCESILSIDDERDEMDKGHLKSNQSDTEMTNMEELTHIVSSVSDESTYPPLHLAASLKNSVPSTLIISEESKPLAQVQDLTSPTHLVTLLGSCSKCSPQEDKSKEDINSMTLSPCSTPLGRPLLDELPRFTNQRPSNLPSTTGTRSPFKNWNSSSPVAQRVPHTSLRTEDHTQDITSYGLSPHKQSSSQAFNDKSSITPLGGEHKLDTKPPSAFSPSLRMRSPSPIRGTQSSRPTSTTSALTKSLAASCISQTISQSMAKRGAHIQTVSQPVRSSPLPTSSVRLRTPSPKPITLDSNVESGFRAISSPGVGSQPPRSCPSPFRSNSLRSSPATVQSPPPYRSQRSISPAPPVSLHSTPSVNSPTSTALEQPSFSSLNCNNNNNNSLSNNGWVANNKKTAPSAVSGLPHFHDSQRTTSHNRVARPFPSSEPSSRVQSPSICLQSPITRICSPPPAPNHSSHLVTKPPNPRASRQGGYFTPLSLEISRSTSACSISPCGSPRITSPPPIGIPTNVWGYANPQPRNPLVTSASSHTKLETNSTPRGSRIISPTPTGSSSYSANSQSQRRLRGTSLPFVVIGDSLPSPTRHERRSWAESGRWSVGSDLGLISPRGGSYSGSPSSVSPGPLSPVRLTAERTSGKHFTGISWPDVHDLLIKYTEPNTEANALSCTEIEPGDMDGTEPTCRSTLICPYVAPASPIERDTPIQCQTEAKTEDCVPNQGSKTTLKTSYATTVNLQIAGSGRIASFSNAQVSLTQTLTPVADSQSRRRVSINSCNFSMQNCKRL

Target ORF information:

RefSeq Version	XM_689253.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (plekhg2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_689253.8

ORF Insert Sequence:

ATGCCTGAAG GTGCGGGACG CAACAGCCAG AAAAAAAACA GCAACCAAGC TGCCAAGCGC 
CCCTCCTCTG TGTCCTCGCT GAGTGGGATT GTGAGCCGCA TGGCCTCCTC AGGAGGCGCA 
TCCCGCGGGA GCTGCACCTC CGTTAACACA GTGTGCTCTG ACAGTGACCG TGCTGCTAGT 
CTAAGCTCCT CTGCCTCCTC TGCCTCGCTG CAGGATGGAC ACTCTTCGTC TTCGTCCTCA 
TCGCTCCCCT ACGGAGCAGT GCCAGCCTAC CCTGGGCCAC AGCATAATGG TTCTGATATC 
AGCCTGGACC TCACCCCTCT CTCCCTGCTG GCGGGAGCCG GTGCCCTCTC CACTTCCACA 
ACAACACTGC CCAAACTTAC TCGCCTGGAG AGGGTGGCGT TAGAGATACT GGAGACCGAA 
CAGGCCTATG TGAGGGACCT CAAGAGCATT GTGGAGGACT ATCTTGGCTG CATTATTGAC 
TGTGGCGACT TGCCGCTCAA ACCCGAAGAG GTCAGCACCC TCTTCTGCAA CATCGAGGAC 
ATCTATGAGT TTAACAGTGA GCTTTTGGAA GATCTGGAGC GCAGTCCGCA TGCGGCAGCC 
ATAGCAGAGT GCTTTGTGGA GAGGAGTGAA GCTTTTGACA TTTACACCAT ATACTGCATG 
AATTACCCCA ACTCAGTGAC CGTCCTGCGG GACTGCATGA AGAACGAGAG CCTGGTGCGC 
TTCTTCCAGG AAAGACAGGC CACCTTGTGT CACTCGCTGC CTCTGGAGAC CTACCTGCTA 
AAACCAGTGC AGAGAATCCT TAAATACCAC CTCCTGCTGC AGGAGCTGTC CAAACACTTT 
GATAAAAGTG ACCCTGGCTA TGAGGTGGTG GAAGATGCCA TTATAACTAT GACAGCAGTG 
GCCTGGTACA TCAATGACAT GAAGAGGAAG CAGGAACATG CAATCAGATT GCAGGAGATT 
GAGAGCTTGC TGCTGAACTG GACGGGTCCT GACCTCAGTG GTTTTGGAGA GCTGGTTCTT 
GAAGGTTCCT TCCGGGTTCA ACGCGTGAAA AAGGAAAGAG CTTTCTTTCT TTTTGACAAA 
ATGCTTCTTA TTGCCAAGAA GAGGCTGGAT CATTTCATAT ACAGTACTCA CATATTTTGC 
TGTAATCTGC TCTTAGTGGA AAATATGAAG GATCCCCTCT GTTTTAAGGT GTCTGACCAA 
ACAATCCCAA AACAACAACA TGTTGTTCAG GCTAAAAACC AGGAGGAGAA GCGACTGTGG 
CTACATTACC TCAAGAGATT GATCGTAGAA AACCATCCTG CATCCTTGCC TCAAAAAGCG 
AGGCAGGTTC TTGGTGACAA CTTCTGTCAG TCTCCACAGT TTGATCAGGA ACCAATTAGA 
AAGCCAATGC CGTCACCACG ACTGGATGAT GTTCATAACT ACCACAGAGG AAGAAGGCAA 
TCAGAGCCAC CAGAATTCAT CTACACCCCT GAAAAAGCCA AGAAAAGCCT TCCCATGCTG 
TTAGAGGGTA ACCTGCCATA CAAACGTGGA AGAAGACAAT CTGCGCCTGC CAAAGATATA 
GAAGCTGCAT TTCAGCAAAG CGGTTCTTTA AAGAGTTCTC AAGCTGGCAG TGAAGGAGAA 
TTGTGCCCAC AGGCTGATAG TATGGGCTCT TCAGGCAGTA CCAGTACTCT GGCTTCCTCA 
GTCATTGAAG TTGAATCTGG ACGTGATGAT CTGGCCTTAT GTCAAGATGA AGATGATATG 
ACACCACTCC CTCCACCACC AACACTGTCT ATTACTGAAG AAATCATGGA ATTCATTAAC 
CAGAGCCGTG CTCGGCAGGG CATGACAGAG TTGACATCTG ATAGGTGTCA GTTGATTCCA 
GAGACCTTAG AAGTTCAGCC ATTGGAAGGA CAGGAGTCAG AGGAATCCAA ACCACTTATA 
GCTGAGATGG ATGAAAATAC ACATGCGATC ATTAATGTCC AAAGATCCGA TGCAGAGAAA 
ACTGAATCAG TTCAAGACGA GGACAGCCAT CCATCCTGTG AAGAAAATAT TTCTCAACCA 
AATGAGCCAA AGGGAGTATC ACCAACTCTG CATGTCTCTA CTGAGGAAAA TCAATTAGAA 
GAGCTGACAT TCCAAGAGGC CAGTTGTGGT GAATCATGCA AATCAGAGAC CTTAAGTGAA 
GGAGAGAAAG ATAATGAGAA CTCTGTTAGT AAAAGTGAAA ATAAATCAGC TAAATCTGCA 
AATGAGACAG CATGTGAGGC CAATTCACAT TGTCCTCTTA TTGATACTTC AGAGAAAGAA 
TCTGAAAGCT CTCCCAAAAT AGAACCTGAG CAAAAACTGA CCAAAAGTGA CAAGCAGATT 
ATTGAGAAGA TTCGCAGCTA TTATGAGGCA GCGGAGACAG GAGTTGAGGA AGGTCAGTCT 
ACACGAAGAA ACAGCTTTTC TCATATTCCT GCAGGATTAG TGAAGGATTC TGTAACACGA 
TTCAATGTTT GTGTTCGTCA GGACAGTTTG TTTGAGTCTG AGAGTGGCCG CTCAGACACT 
GTTGACACTG ACTCAGCATT TTTAATACTC CCTGTATCTG ACCAAGCTGA CCAGACTTTC 
AGCCATCAGG AATCTGTTGA TGCAGACATT CCTCCCTCAC ATTCAGATGC TGACCATCAA 
GTGGAAGAAA TTGACGGCAA GACCTCAATG AAAAAGGATG AAGATGTTTG TGATTTCAGT 
ATGAAACTGT GGAAGGAGAA TGAAAAGACT GGCCAAGAGT CTCCAAGCAG TCCGAAAGTC 
CCCATCTCAA GAGAGAACAG CTGTGGTGAA CATACAGATG CTCCTGAGAA ATTGTCCGTA 
AATTGCATAA CAACCCAAGT ACAGCATAAA CAATCGGAGA GTTTACAATG TGATCCAGAC 
GCACCAGACA AATCTAATCT GCAGGCCACA GAGACAGCAG ATGCATCACT TCAATGTGCA 
AGTAAAACTA GATCTAGGAT GTCTTCTAAT GTAAGCCTGG ATAGCCTTCC CATTCAGATT 
AAAGTTGGCA GATGGTCTCG TCATGGCAAG GTGGTTACAT GTAGCCAAAC CCTTTATGAA 
GGAATGGCCG AGGTACCAGA TTTAGGATTT TTTGAGAGTG GACCAGTCAA TCAGTGCTTG 
GTGGAAAACT CAGAAAAGAT TTTAAATAAA GTGCAAATGC TTGCACGCAT GTACACTGCC 
AAGGCGAGCA GCATGAAGGT ACCGCTGCAT CAGAAAAAAA CACGAGTGAG TAGGGGAGCA 
TGGTCAATGG ATGGAAAAGG GAGCACTTCA CCTAAACCTC AGCAAGTAAA GCTGCACCAG 
GGGGATATGA GGATAAGCCA GTCTGAAGAA GAATCTTTGG AAACTACACC TACTGAGCCT 
TTTGGTCATA TAATTTTAAG GGAGAAGCTG TCTACAACTT ATCACCAAGA AAATGATTGC 
AATCTTATTG GCCCGCTAGA TGAGATTTCA GAAACTGGAT CTAATTCACT GTCTTTTTCT 
TCTGCTGATT CCTCTTTCAC TTCGGCTCAA ATACTAGCAA CTGTTACAGA ATCAAAGGTC 
TCTGTGATAT CAGACCATAC AGACAGGTCA TTAGAAATGA CAGAGTCGAA ACTACATGAA 
AAAGAACAAT CCGTACCACT TCCAGTTTAC TCTGGAACAC CAGTCCAAGC TGAGTCAGTA 
GTCCCATCCA AAATCTGTGG GTTGCCATTG GTTGAGCATG CTAGCAGTGT ACTACAGTCC 
ATTCAGGAGA ACCATTTAGA TGTTGGGGAC AAATTAAATA CAGTATATAG AATTGCAGAA 
AGACAGGTAT TGCAAGAGGA TAAACCTGCT TTTGTAAACA ATTATTGTCC AATTAGTGAT 
GTTTTTATTG ACCCACTAGT CAATAAACAT GTAACTAAAG AGACTCACAG TGAGGAGAAA 
AAAGACTCTG TTGTACAGCA CTGTGAAAGT ATATTGTCTA TAGATGATGA ACGAGATGAA 
ATGGATAAAG GACATTTGAA ATCCAACCAG TCTGATACTG AAATGACTAA CATGGAGGAA 
CTAACACATA TAGTGTCCTC CGTTAGTGAT GAAAGCACAT ATCCTCCATT ACACTTAGCA 
GCCTCCTTAA AAAACAGTGT TCCATCCACT TTGATAATCT CTGAGGAATC AAAACCTTTG 
GCCCAGGTGC AGGATTTAAC TAGCCCTACG CATCTGGTGA CTCTGCTTGG TTCATGTAGC 
AAGTGTAGTC CACAGGAAGA TAAATCTAAA GAAGACATTA ATTCAATGAC TTTGAGCCCA 
TGCTCAACCC CACTTGGTAG ACCTCTTTTA GATGAGCTAC CAAGGTTTAC AAATCAAAGA 
CCCTCCAATC TGCCTTCCAC CACAGGAACG AGAAGTCCCT TCAAAAATTG GAATTCATCA 
TCTCCAGTTG CTCAAAGAGT GCCACACACT TCACTGCGAA CAGAGGATCA CACTCAAGAT 
ATTACTTCTT ATGGCCTTTC TCCTCACAAG CAGTCTTCAT CCCAGGCTTT TAATGATAAG 
TCTTCAATCA CTCCCTTGGG TGGTGAACAT AAATTAGATA CAAAACCACC CTCTGCCTTT 
AGTCCAAGTC TCCGAATGAG GTCACCCTCT CCAATTAGAG GTACACAGAG CTCCAGACCT 
ACTTCAACAA CCTCAGCTCT TACAAAGTCT CTTGCTGCCT CCTGCATCAG TCAGACGATT 
TCTCAAAGCA TGGCCAAGAG AGGTGCACAT ATTCAGACTG TGTCCCAACC TGTAAGATCC 
TCTCCTCTAC CCACATCTTC CGTAAGATTA CGGACGCCAT CACCAAAACC CATCACCTTG 
GACTCAAATG TAGAGTCAGG ATTTAGGGCA ATATCCAGCC CTGGGGTAGG AAGTCAACCC 
CCAAGGTCTT GTCCATCCCC ATTCAGATCC AACAGTCTTC GATCTAGTCC AGCAACAGTC 
CAGTCCCCTC CTCCTTATCG TAGCCAAAGG TCAATATCTC CAGCACCTCC AGTTAGCCTC 
CATTCAACTC CATCTGTTAA CAGCCCAACC TCCACTGCTC TTGAACAACC TTCGTTCAGC 
AGTTTAAATT GTAATAATAA CAATAATAAC AGCTTAAGTA ATAATGGGTG GGTCGCTAAT 
AACAAAAAAA CAGCACCATC AGCTGTAAGT GGACTCCCTC ATTTCCATGA TTCCCAGCGA 
ACAACCTCAC ACAATAGGGT GGCTCGTCCT TTCCCATCGT CTGAGCCTAG TTCCCGTGTA 
CAGTCCCCTT CCATTTGCCT CCAATCTCCA ATCACCCGAA TCTGCTCTCC TCCACCTGCT 
CCCAACCATT CCAGTCATCT GGTAACAAAG CCACCCAACC CCAGAGCATC CAGACAAGGT 
GGCTACTTTA CACCTTTATC TTTGGAAATT TCAAGATCAA CATCAGCTTG TTCAATATCT 
CCTTGCGGGA GTCCCAGAAT CACCTCTCCT CCCCCTATTG GCATTCCTAC AAATGTTTGG 
GGCTATGCTA ATCCACAACC AAGGAATCCA TTAGTAACAA GTGCATCGTC TCATACAAAA 
TTGGAAACAA ACTCTACTCC AAGAGGCTCT AGAATCATCT CGCCCACCCC AACAGGATCA 
TCATCCTATT CAGCCAACTC TCAGAGTCAG AGGAGGTTAC GAGGAACCAG TTTGCCCTTT 
GTTGTGATAG GGGATAGCCT TCCAAGTCCA ACTAGACACG AACGCAGATC TTGGGCTGAA 
AGTGGACGGT GGTCAGTGGG TTCAGATCTA GGTTTAATAA GTCCTCGTGG GGGTTCATAC 
AGTGGTAGCC CGTCTTCTGT TAGCCCTGGT CCTCTTTCAC CAGTCAGACT AACAGCCGAA 
AGGACAAGTG GAAAACACTT TACAGGCATT TCCTGGCCAG ATGTACACGA TCTCTTGATT 
AAATACACAG AGCCCAATAC TGAAGCAAAT GCTTTATCCT GCACCGAGAT AGAGCCAGGT 
GACATGGACG GTACAGAACC TACATGTCGG AGTACCCTTA TTTGTCCATA TGTTGCTCCA 
GCCAGCCCCA TTGAGAGAGA CACTCCCATT CAGTGCCAGA CCGAGGCTAA AACAGAAGAC 
TGTGTTCCTA ATCAAGGGTC TAAAACAACA TTAAAGACCA GTTATGCCAC CACTGTCAAT 
TTGCAGATTG CTGGGAGTGG GAGGATTGCA TCCTTTAGCA ATGCACAAGT CAGCCTGACT 
CAAACTCTGA CCCCTGTGGC AGACAGTCAG AGCAGGAGAA GAGTCAGCAT CAACAGCTGC 
AACTTCTCTA TGCAGAACTG CAAAAGGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHG2 ( XM_009291706.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009289981.1 Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (plekhg2), transcript variant X2, mRNA.

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CloneID	ODa20413
Clone ID Related Accession (Same CDS sequence)	XM_009291706.2
Accession Version	XM_009291706.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family G member 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291706.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCCT CAGGAGGCGC ATCCCGCGGG AGCTGCACCT CCGTTAACAC AGTGTGCTCT 
GACAGTGACC GTGCTGCTAG TCTAAGCTCC TCTGCCTCCT CTGCCTCGCT GCAGGATGGA 
CACTCTTCGT CTTCGTCCTC ATCGCTCCCC TACGGAGCAG TGCCAGCCTA CCCTGGGCCA 
CAGCATAATG GTTCTGATAT CAGCCTGGAC CTCACCCCTC TCTCCCTGCT GGCGGGAGCC 
GGTGCCCTCT CCACTTCCAC AACAACACTG CCCAAACTTA CTCGCCTGGA GAGGGTGGCG 
TTAGAGATAC TGGAGACCGA ACAGGCCTAT GTGAGGGACC TCAAGAGCAT TGTGGAGGAC 
TATCTTGGCT GCATTATTGA CTGTGGCGAC TTGCCGCTCA AACCCGAAGA GGTCAGCACC 
CTCTTCTGCA ACATCGAGGA CATCTATGAG TTTAACAGTG AGCTTTTGGA AGATCTGGAG 
CGCAGTCCGC ATGCGGCAGC CATAGCAGAG TGCTTTGTGG AGAGGAGTGA AGCTTTTGAC 
ATTTACACCA TATACTGCAT GAATTACCCC AACTCAGTGA CCGTCCTGCG GGACTGCATG 
AAGAACGAGA GCCTGGTGCG CTTCTTCCAG GAAAGACAGG CCACCTTGTG TCACTCGCTG 
CCTCTGGAGA CCTACCTGCT AAAACCAGTG CAGAGAATCC TTAAATACCA CCTCCTGCTG 
CAGGAGCTGT CCAAACACTT TGATAAAAGT GACCCTGGCT ATGAGGTGGT GGAAGATGCC 
ATTATAACTA TGACAGCAGT GGCCTGGTAC ATCAATGACA TGAAGAGGAA GCAGGAACAT 
GCAATCAGAT TGCAGGAGAT TGAGAGCTTG CTGCTGAACT GGACGGGTCC TGACCTCAGT 
GGTTTTGGAG AGCTGGTTCT TGAAGGTTCC TTCCGGGTTC AACGCGTGAA AAAGGAAAGA 
GCTTTCTTTC TTTTTGACAA AATGCTTCTT ATTGCCAAGA AGAGGCTGGA TCATTTCATA 
TACAGTACTC ACATATTTTG CTGTAATCTG CTCTTAGTGG AAAATATGAA GGATCCCCTC 
TGTTTTAAGG TGTCTGACCA AACAATCCCA AAACAACAAC ATGTTGTTCA GGCTAAAAAC 
CAGGAGGAGA AGCGACTGTG GCTACATTAC CTCAAGAGAT TGATCGTAGA AAACCATCCT 
GCATCCTTGC CTCAAAAAGC GAGGCAGGTT CTTGGTGACA ACTTCTGTCA GTCTCCACAG 
TTTGATCAGG AACCAATTAG AAAGCCAATG CCGTCACCAC GACTGGATGA TGTTCATAAC 
TACCACAGAG GAAGAAGGCA ATCAGAGCCA CCAGAATTCA TCTACACCCC TGAAAAAGCC 
AAGAAAAGCC TTCCCATGCT GTTAGAGGGT AACCTGCCAT ACAAACGTGG AAGAAGACAA 
TCTGCGCCTG CCAAAGATAT AGAAGCTGCA TTTCAGCAAA GCGGTTCTTT AAAGAGTTCT 
CAAGCTGGCA GTGAAGGAGA ATTGTGCCCA CAGGCTGATA GTATGGGCTC TTCAGGCAGT 
ACCAGTACTC TGGCTTCCTC AGTCATTGAA GTTGAATCTG GACGTGATGA TCTGGCCTTA 
TGTCAAGATG AAGATGATAT GACACCACTC CCTCCACCAC CAACACTGTC TATTACTGAA 
GAAATCATGG AATTCATTAA CCAGAGCCGT GCTCGGCAGG GCATGACAGA GTTGACATCT 
GATAGGTGTC AGTTGATTCC AGAGACCTTA GAAGTTCAGC CATTGGAAGG ACAGGAGTCA 
GAGGAATCCA AACCACTTAT AGCTGAGATG GATGAAAATA CACATGCGAT CATTAATGTC 
CAAAGATCCG ATGCAGAGAA AACTGAATCA GTTCAAGACG AGGACAGCCA TCCATCCTGT 
GAAGAAAATA TTTCTCAACC AAATGAGCCA AAGGGAGTAT CACCAACTCT GCATGTCTCT 
ACTGAGGAAA ATCAATTAGA AGAGCTGACA TTCCAAGAGG CCAGTTGTGG TGAATCATGC 
AAATCAGAGA CCTTAAGTGA AGGAGAGAAA GATAATGAGA ACTCTGTTAG TAAAAGTGAA 
AATAAATCAG CTAAATCTGC AAATGAGACA GCATGTGAGG CCAATTCACA TTGTCCTCTT 
ATTGATACTT CAGAGAAAGA ATCTGAAAGC TCTCCCAAAA TAGAACCTGA GCAAAAACTG 
ACCAAAAGTG ACAAGCAGAT TATTGAGAAG ATTCGCAGCT ATTATGAGGC AGCGGAGACA 
GGAGTTGAGG AAGGTCAGTC TACACGAAGA AACAGCTTTT CTCATATTCC TGCAGGATTA 
GTGAAGGATT CTGTAACACG ATTCAATGTT TGTGTTCGTC AGGACAGTTT GTTTGAGTCT 
GAGAGTGGCC GCTCAGACAC TGTTGACACT GACTCAGCAT TTTTAATACT CCCTGTATCT 
GACCAAGCTG ACCAGACTTT CAGCCATCAG GAATCTGTTG ATGCAGACAT TCCTCCCTCA 
CATTCAGATG CTGACCATCA AGTGGAAGAA ATTGACGGCA AGACCTCAAT GAAAAAGGAT 
GAAGATGTTT GTGATTTCAG TATGAAACTG TGGAAGGAGA ATGAAAAGAC TGGCCAAGAG 
TCTCCAAGCA GTCCGAAAGT CCCCATCTCA AGAGAGAACA GCTGTGGTGA ACATACAGAT 
GCTCCTGAGA AATTGTCCGT AAATTGCATA ACAACCCAAG TACAGCATAA ACAATCGGAG 
AGTTTACAAT GTGATCCAGA CGCACCAGAC AAATCTAATC TGCAGGCCAC AGAGACAGCA 
GATGCATCAC TTCAATGTGC AAGTAAAACT AGATCTAGGA TGTCTTCTAA TGTAAGCCTG 
GATAGCCTTC CCATTCAGAT TAAAGTTGGC AGATGGTCTC GTCATGGCAA GGTGGTTACA 
TGTAGCCAAA CCCTTTATGA AGGAATGGCC GAGGTACCAG ATTTAGGATT TTTTGAGAGT 
GGACCAGTCA ATCAGTGCTT GGTGGAAAAC TCAGAAAAGA TTTTAAATAA AGTGCAAATG 
CTTGCACGCA TGTACACTGC CAAGGCGAGC AGCATGAAGG TACCGCTGCA TCAGAAAAAA 
ACACGAGTGA GTAGGGGAGC ATGGTCAATG GATGGAAAAG GGAGCACTTC ACCTAAACCT 
CAGCAAGTAA AGCTGCACCA GGGGGATATG AGGATAAGCC AGTCTGAAGA AGAATCTTTG 
GAAACTACAC CTACTGAGCC TTTTGGTCAT ATAATTTTAA GGGAGAAGCT GTCTACAACT 
TATCACCAAG AAAATGATTG CAATCTTATT GGCCCGCTAG ATGAGATTTC AGAAACTGGA 
TCTAATTCAC TGTCTTTTTC TTCTGCTGAT TCCTCTTTCA CTTCGGCTCA AATACTAGCA 
ACTGTTACAG AATCAAAGGT CTCTGTGATA TCAGACCATA CAGACAGGTC ATTAGAAATG 
ACAGAGTCGA AACTACATGA AAAAGAACAA TCCGTACCAC TTCCAGTTTA CTCTGGAACA 
CCAGTCCAAG CTGAGTCAGT AGTCCCATCC AAAATCTGTG GGTTGCCATT GGTTGAGCAT 
GCTAGCAGTG TACTACAGTC CATTCAGGAG AACCATTTAG ATGTTGGGGA CAAATTAAAT 
ACAGTATATA GAATTGCAGA AAGACAGGTA TTGCAAGAGG ATAAACCTGC TTTTGTAAAC 
AATTATTGTC CAATTAGTGA TGTTTTTATT GACCCACTAG TCAATAAACA TGTAACTAAA 
GAGACTCACA GTGAGGAGAA AAAAGACTCT GTTGTACAGC ACTGTGAAAG TATATTGTCT 
ATAGATGATG AACGAGATGA AATGGATAAA GGACATTTGA AATCCAACCA GTCTGATACT 
GAAATGACTA ACATGGAGGA ACTAACACAT ATAGTGTCCT CCGTTAGTGA TGAAAGCACA 
TATCCTCCAT TACACTTAGC AGCCTCCTTA AAAAACAGTG TTCCATCCAC TTTGATAATC 
TCTGAGGAAT CAAAACCTTT GGCCCAGGTG CAGGATTTAA CTAGCCCTAC GCATCTGGTG 
ACTCTGCTTG GTTCATGTAG CAAGTGTAGT CCACAGGAAG ATAAATCTAA AGAAGACATT 
AATTCAATGA CTTTGAGCCC ATGCTCAACC CCACTTGGTA GACCTCTTTT AGATGAGCTA 
CCAAGGTTTA CAAATCAAAG ACCCTCCAAT CTGCCTTCCA CCACAGGAAC GAGAAGTCCC 
TTCAAAAATT GGAATTCATC ATCTCCAGTT GCTCAAAGAG TGCCACACAC TTCACTGCGA 
ACAGAGGATC ACACTCAAGA TATTACTTCT TATGGCCTTT CTCCTCACAA GCAGTCTTCA 
TCCCAGGCTT TTAATGATAA GTCTTCAATC ACTCCCTTGG GTGGTGAACA TAAATTAGAT 
ACAAAACCAC CCTCTGCCTT TAGTCCAAGT CTCCGAATGA GGTCACCCTC TCCAATTAGA 
GGTACACAGA GCTCCAGACC TACTTCAACA ACCTCAGCTC TTACAAAGTC TCTTGCTGCC 
TCCTGCATCA GTCAGACGAT TTCTCAAAGC ATGGCCAAGA GAGGTGCACA TATTCAGACT 
GTGTCCCAAC CTGTAAGATC CTCTCCTCTA CCCACATCTT CCGTAAGATT ACGGACGCCA 
TCACCAAAAC CCATCACCTT GGACTCAAAT GTAGAGTCAG GATTTAGGGC AATATCCAGC 
CCTGGGGTAG GAAGTCAACC CCCAAGGTCT TGTCCATCCC CATTCAGATC CAACAGTCTT 
CGATCTAGTC CAGCAACAGT CCAGTCCCCT CCTCCTTATC GTAGCCAAAG GTCAATATCT 
CCAGCACCTC CAGTTAGCCT CCATTCAACT CCATCTGTTA ACAGCCCAAC CTCCACTGCT 
CTTGAACAAC CTTCGTTCAG CAGTTTAAAT TGTAATAATA ACAATAATAA CAGCTTAAGT 
AATAATGGGT GGGTCGCTAA TAACAAAAAA ACAGCACCAT CAGCTGTAAG TGGACTCCCT 
CATTTCCATG ATTCCCAGCG AACAACCTCA CACAATAGGG TGGCTCGTCC TTTCCCATCG 
TCTGAGCCTA GTTCCCGTGT ACAGTCCCCT TCCATTTGCC TCCAATCTCC AATCACCCGA 
ATCTGCTCTC CTCCACCTGC TCCCAACCAT TCCAGTCATC TGGTAACAAA GCCACCCAAC 
CCCAGAGCAT CCAGACAAGG TGGCTACTTT ACACCTTTAT CTTTGGAAAT TTCAAGATCA 
ACATCAGCTT GTTCAATATC TCCTTGCGGG AGTCCCAGAA TCACCTCTCC TCCCCCTATT 
GGCATTCCTA CAAATGTTTG GGGCTATGCT AATCCACAAC CAAGGAATCC ATTAGTAACA 
AGTGCATCGT CTCATACAAA ATTGGAAACA AACTCTACTC CAAGAGGCTC TAGAATCATC 
TCGCCCACCC CAACAGGATC ATCATCCTAT TCAGCCAACT CTCAGAGTCA GAGGAGGTTA 
CGAGGAACCA GTTTGCCCTT TGTTGTGATA GGGGATAGCC TTCCAAGTCC AACTAGACAC 
GAACGCAGAT CTTGGGCTGA AAGTGGACGG TGGTCAGTGG GTTCAGATCT AGGTTTAATA 
AGTCCTCGTG GGGGTTCATA CAGTGGTAGC CCGTCTTCTG TTAGCCCTGG TCCTCTTTCA 
CCAGTCAGAC TAACAGCCGA AAGGACAAGT GGAAAACACT TTACAGGCAT TTCCTGGCCA 
GATGTACACG ATCTCTTGAT TAAATACACA GAGCCCAATA CTGAAGCAAA TGCTTTATCC 
TGCACCGAGA TAGAGCCAGG TGACATGGAC GGTACAGAAC CTACATGTCG GAGTACCCTT 
ATTTGTCCAT ATGTTGCTCC AGCCAGCCCC ATTGAGAGAG ACACTCCCAT TCAGTGCCAG 
ACCGAGGCTA AAACAGAAGA CTGTGTTCCT AATCAAGGGT CTAAAACAAC ATTAAAGACC 
AGTTATGCCA CCACTGTCAA TTTGCAGATT GCTGGGAGTG GGAGGATTGC ATCCTTTAGC 
AATGCACAAG TCAGCCTGAC TCAAACTCTG ACCCCTGTGG CAGACAGTCA GAGCAGGAGA 
AGAGTCAGCA TCAACAGCTG CAACTTCTCT ATGCAGAACT GCAAAAGGCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289981.1
CDS	474..6647
Translation	MASSGGASRGSCTSVNTVCSDSDRAASLSSSASSASLQDGHSSSSSSSLPYGAVPAYPGPQHNGSDISLDLTPLSLLAGAGALSTSTTTLPKLTRLERVALEILETEQAYVRDLKSIVEDYLGCIIDCGDLPLKPEEVSTLFCNIEDIYEFNSELLEDLERSPHAAAIAECFVERSEAFDIYTIYCMNYPNSVTVLRDCMKNESLVRFFQERQATLCHSLPLETYLLKPVQRILKYHLLLQELSKHFDKSDPGYEVVEDAIITMTAVAWYINDMKRKQEHAIRLQEIESLLLNWTGPDLSGFGELVLEGSFRVQRVKKERAFFLFDKMLLIAKKRLDHFIYSTHIFCCNLLLVENMKDPLCFKVSDQTIPKQQHVVQAKNQEEKRLWLHYLKRLIVENHPASLPQKARQVLGDNFCQSPQFDQEPIRKPMPSPRLDDVHNYHRGRRQSEPPEFIYTPEKAKKSLPMLLEGNLPYKRGRRQSAPAKDIEAAFQQSGSLKSSQAGSEGELCPQADSMGSSGSTSTLASSVIEVESGRDDLALCQDEDDMTPLPPPPTLSITEEIMEFINQSRARQGMTELTSDRCQLIPETLEVQPLEGQESEESKPLIAEMDENTHAIINVQRSDAEKTESVQDEDSHPSCEENISQPNEPKGVSPTLHVSTEENQLEELTFQEASCGESCKSETLSEGEKDNENSVSKSENKSAKSANETACEANSHCPLIDTSEKESESSPKIEPEQKLTKSDKQIIEKIRSYYEAAETGVEEGQSTRRNSFSHIPAGLVKDSVTRFNVCVRQDSLFESESGRSDTVDTDSAFLILPVSDQADQTFSHQESVDADIPPSHSDADHQVEEIDGKTSMKKDEDVCDFSMKLWKENEKTGQESPSSPKVPISRENSCGEHTDAPEKLSVNCITTQVQHKQSESLQCDPDAPDKSNLQATETADASLQCASKTRSRMSSNVSLDSLPIQIKVGRWSRHGKVVTCSQTLYEGMAEVPDLGFFESGPVNQCLVENSEKILNKVQMLARMYTAKASSMKVPLHQKKTRVSRGAWSMDGKGSTSPKPQQVKLHQGDMRISQSEEESLETTPTEPFGHIILREKLSTTYHQENDCNLIGPLDEISETGSNSLSFSSADSSFTSAQILATVTESKVSVISDHTDRSLEMTESKLHEKEQSVPLPVYSGTPVQAESVVPSKICGLPLVEHASSVLQSIQENHLDVGDKLNTVYRIAERQVLQEDKPAFVNNYCPISDVFIDPLVNKHVTKETHSEEKKDSVVQHCESILSIDDERDEMDKGHLKSNQSDTEMTNMEELTHIVSSVSDESTYPPLHLAASLKNSVPSTLIISEESKPLAQVQDLTSPTHLVTLLGSCSKCSPQEDKSKEDINSMTLSPCSTPLGRPLLDELPRFTNQRPSNLPSTTGTRSPFKNWNSSSPVAQRVPHTSLRTEDHTQDITSYGLSPHKQSSSQAFNDKSSITPLGGEHKLDTKPPSAFSPSLRMRSPSPIRGTQSSRPTSTTSALTKSLAASCISQTISQSMAKRGAHIQTVSQPVRSSPLPTSSVRLRTPSPKPITLDSNVESGFRAISSPGVGSQPPRSCPSPFRSNSLRSSPATVQSPPPYRSQRSISPAPPVSLHSTPSVNSPTSTALEQPSFSSLNCNNNNNNSLSNNGWVANNKKTAPSAVSGLPHFHDSQRTTSHNRVARPFPSSEPSSRVQSPSICLQSPITRICSPPPAPNHSSHLVTKPPNPRASRQGGYFTPLSLEISRSTSACSISPCGSPRITSPPPIGIPTNVWGYANPQPRNPLVTSASSHTKLETNSTPRGSRIISPTPTGSSSYSANSQSQRRLRGTSLPFVVIGDSLPSPTRHERRSWAESGRWSVGSDLGLISPRGGSYSGSPSSVSPGPLSPVRLTAERTSGKHFTGISWPDVHDLLIKYTEPNTEANALSCTEIEPGDMDGTEPTCRSTLICPYVAPASPIERDTPIQCQTEAKTEDCVPNQGSKTTLKTSYATTVNLQIAGSGRIASFSNAQVSLTQTLTPVADSQSRRRVSINSCNFSMQNCKRL

Target ORF information:

RefSeq Version	XM_009291706.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (plekhg2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291706.2

ORF Insert Sequence:

ATGGCCTCCT CAGGAGGCGC ATCCCGCGGG AGCTGCACCT CCGTTAACAC AGTGTGCTCT 
GACAGTGACC GTGCTGCTAG TCTAAGCTCC TCTGCCTCCT CTGCCTCGCT GCAGGATGGA 
CACTCTTCGT CTTCGTCCTC ATCGCTCCCC TACGGAGCAG TGCCAGCCTA CCCTGGGCCA 
CAGCATAATG GTTCTGATAT CAGCCTGGAC CTCACCCCTC TCTCCCTGCT GGCGGGAGCC 
GGTGCCCTCT CCACTTCCAC AACAACACTG CCCAAACTTA CTCGCCTGGA GAGGGTGGCG 
TTAGAGATAC TGGAGACCGA ACAGGCCTAT GTGAGGGACC TCAAGAGCAT TGTGGAGGAC 
TATCTTGGCT GCATTATTGA CTGTGGCGAC TTGCCGCTCA AACCCGAAGA GGTCAGCACC 
CTCTTCTGCA ACATCGAGGA CATCTATGAG TTTAACAGTG AGCTTTTGGA AGATCTGGAG 
CGCAGTCCGC ATGCGGCAGC CATAGCAGAG TGCTTTGTGG AGAGGAGTGA AGCTTTTGAC 
ATTTACACCA TATACTGCAT GAATTACCCC AACTCAGTGA CCGTCCTGCG GGACTGCATG 
AAGAACGAGA GCCTGGTGCG CTTCTTCCAG GAAAGACAGG CCACCTTGTG TCACTCGCTG 
CCTCTGGAGA CCTACCTGCT AAAACCAGTG CAGAGAATCC TTAAATACCA CCTCCTGCTG 
CAGGAGCTGT CCAAACACTT TGATAAAAGT GACCCTGGCT ATGAGGTGGT GGAAGATGCC 
ATTATAACTA TGACAGCAGT GGCCTGGTAC ATCAATGACA TGAAGAGGAA GCAGGAACAT 
GCAATCAGAT TGCAGGAGAT TGAGAGCTTG CTGCTGAACT GGACGGGTCC TGACCTCAGT 
GGTTTTGGAG AGCTGGTTCT TGAAGGTTCC TTCCGGGTTC AACGCGTGAA AAAGGAAAGA 
GCTTTCTTTC TTTTTGACAA AATGCTTCTT ATTGCCAAGA AGAGGCTGGA TCATTTCATA 
TACAGTACTC ACATATTTTG CTGTAATCTG CTCTTAGTGG AAAATATGAA GGATCCCCTC 
TGTTTTAAGG TGTCTGACCA AACAATCCCA AAACAACAAC ATGTTGTTCA GGCTAAAAAC 
CAGGAGGAGA AGCGACTGTG GCTACATTAC CTCAAGAGAT TGATCGTAGA AAACCATCCT 
GCATCCTTGC CTCAAAAAGC GAGGCAGGTT CTTGGTGACA ACTTCTGTCA GTCTCCACAG 
TTTGATCAGG AACCAATTAG AAAGCCAATG CCGTCACCAC GACTGGATGA TGTTCATAAC 
TACCACAGAG GAAGAAGGCA ATCAGAGCCA CCAGAATTCA TCTACACCCC TGAAAAAGCC 
AAGAAAAGCC TTCCCATGCT GTTAGAGGGT AACCTGCCAT ACAAACGTGG AAGAAGACAA 
TCTGCGCCTG CCAAAGATAT AGAAGCTGCA TTTCAGCAAA GCGGTTCTTT AAAGAGTTCT 
CAAGCTGGCA GTGAAGGAGA ATTGTGCCCA CAGGCTGATA GTATGGGCTC TTCAGGCAGT 
ACCAGTACTC TGGCTTCCTC AGTCATTGAA GTTGAATCTG GACGTGATGA TCTGGCCTTA 
TGTCAAGATG AAGATGATAT GACACCACTC CCTCCACCAC CAACACTGTC TATTACTGAA 
GAAATCATGG AATTCATTAA CCAGAGCCGT GCTCGGCAGG GCATGACAGA GTTGACATCT 
GATAGGTGTC AGTTGATTCC AGAGACCTTA GAAGTTCAGC CATTGGAAGG ACAGGAGTCA 
GAGGAATCCA AACCACTTAT AGCTGAGATG GATGAAAATA CACATGCGAT CATTAATGTC 
CAAAGATCCG ATGCAGAGAA AACTGAATCA GTTCAAGACG AGGACAGCCA TCCATCCTGT 
GAAGAAAATA TTTCTCAACC AAATGAGCCA AAGGGAGTAT CACCAACTCT GCATGTCTCT 
ACTGAGGAAA ATCAATTAGA AGAGCTGACA TTCCAAGAGG CCAGTTGTGG TGAATCATGC 
AAATCAGAGA CCTTAAGTGA AGGAGAGAAA GATAATGAGA ACTCTGTTAG TAAAAGTGAA 
AATAAATCAG CTAAATCTGC AAATGAGACA GCATGTGAGG CCAATTCACA TTGTCCTCTT 
ATTGATACTT CAGAGAAAGA ATCTGAAAGC TCTCCCAAAA TAGAACCTGA GCAAAAACTG 
ACCAAAAGTG ACAAGCAGAT TATTGAGAAG ATTCGCAGCT ATTATGAGGC AGCGGAGACA 
GGAGTTGAGG AAGGTCAGTC TACACGAAGA AACAGCTTTT CTCATATTCC TGCAGGATTA 
GTGAAGGATT CTGTAACACG ATTCAATGTT TGTGTTCGTC AGGACAGTTT GTTTGAGTCT 
GAGAGTGGCC GCTCAGACAC TGTTGACACT GACTCAGCAT TTTTAATACT CCCTGTATCT 
GACCAAGCTG ACCAGACTTT CAGCCATCAG GAATCTGTTG ATGCAGACAT TCCTCCCTCA 
CATTCAGATG CTGACCATCA AGTGGAAGAA ATTGACGGCA AGACCTCAAT GAAAAAGGAT 
GAAGATGTTT GTGATTTCAG TATGAAACTG TGGAAGGAGA ATGAAAAGAC TGGCCAAGAG 
TCTCCAAGCA GTCCGAAAGT CCCCATCTCA AGAGAGAACA GCTGTGGTGA ACATACAGAT 
GCTCCTGAGA AATTGTCCGT AAATTGCATA ACAACCCAAG TACAGCATAA ACAATCGGAG 
AGTTTACAAT GTGATCCAGA CGCACCAGAC AAATCTAATC TGCAGGCCAC AGAGACAGCA 
GATGCATCAC TTCAATGTGC AAGTAAAACT AGATCTAGGA TGTCTTCTAA TGTAAGCCTG 
GATAGCCTTC CCATTCAGAT TAAAGTTGGC AGATGGTCTC GTCATGGCAA GGTGGTTACA 
TGTAGCCAAA CCCTTTATGA AGGAATGGCC GAGGTACCAG ATTTAGGATT TTTTGAGAGT 
GGACCAGTCA ATCAGTGCTT GGTGGAAAAC TCAGAAAAGA TTTTAAATAA AGTGCAAATG 
CTTGCACGCA TGTACACTGC CAAGGCGAGC AGCATGAAGG TACCGCTGCA TCAGAAAAAA 
ACACGAGTGA GTAGGGGAGC ATGGTCAATG GATGGAAAAG GGAGCACTTC ACCTAAACCT 
CAGCAAGTAA AGCTGCACCA GGGGGATATG AGGATAAGCC AGTCTGAAGA AGAATCTTTG 
GAAACTACAC CTACTGAGCC TTTTGGTCAT ATAATTTTAA GGGAGAAGCT GTCTACAACT 
TATCACCAAG AAAATGATTG CAATCTTATT GGCCCGCTAG ATGAGATTTC AGAAACTGGA 
TCTAATTCAC TGTCTTTTTC TTCTGCTGAT TCCTCTTTCA CTTCGGCTCA AATACTAGCA 
ACTGTTACAG AATCAAAGGT CTCTGTGATA TCAGACCATA CAGACAGGTC ATTAGAAATG 
ACAGAGTCGA AACTACATGA AAAAGAACAA TCCGTACCAC TTCCAGTTTA CTCTGGAACA 
CCAGTCCAAG CTGAGTCAGT AGTCCCATCC AAAATCTGTG GGTTGCCATT GGTTGAGCAT 
GCTAGCAGTG TACTACAGTC CATTCAGGAG AACCATTTAG ATGTTGGGGA CAAATTAAAT 
ACAGTATATA GAATTGCAGA AAGACAGGTA TTGCAAGAGG ATAAACCTGC TTTTGTAAAC 
AATTATTGTC CAATTAGTGA TGTTTTTATT GACCCACTAG TCAATAAACA TGTAACTAAA 
GAGACTCACA GTGAGGAGAA AAAAGACTCT GTTGTACAGC ACTGTGAAAG TATATTGTCT 
ATAGATGATG AACGAGATGA AATGGATAAA GGACATTTGA AATCCAACCA GTCTGATACT 
GAAATGACTA ACATGGAGGA ACTAACACAT ATAGTGTCCT CCGTTAGTGA TGAAAGCACA 
TATCCTCCAT TACACTTAGC AGCCTCCTTA AAAAACAGTG TTCCATCCAC TTTGATAATC 
TCTGAGGAAT CAAAACCTTT GGCCCAGGTG CAGGATTTAA CTAGCCCTAC GCATCTGGTG 
ACTCTGCTTG GTTCATGTAG CAAGTGTAGT CCACAGGAAG ATAAATCTAA AGAAGACATT 
AATTCAATGA CTTTGAGCCC ATGCTCAACC CCACTTGGTA GACCTCTTTT AGATGAGCTA 
CCAAGGTTTA CAAATCAAAG ACCCTCCAAT CTGCCTTCCA CCACAGGAAC GAGAAGTCCC 
TTCAAAAATT GGAATTCATC ATCTCCAGTT GCTCAAAGAG TGCCACACAC TTCACTGCGA 
ACAGAGGATC ACACTCAAGA TATTACTTCT TATGGCCTTT CTCCTCACAA GCAGTCTTCA 
TCCCAGGCTT TTAATGATAA GTCTTCAATC ACTCCCTTGG GTGGTGAACA TAAATTAGAT 
ACAAAACCAC CCTCTGCCTT TAGTCCAAGT CTCCGAATGA GGTCACCCTC TCCAATTAGA 
GGTACACAGA GCTCCAGACC TACTTCAACA ACCTCAGCTC TTACAAAGTC TCTTGCTGCC 
TCCTGCATCA GTCAGACGAT TTCTCAAAGC ATGGCCAAGA GAGGTGCACA TATTCAGACT 
GTGTCCCAAC CTGTAAGATC CTCTCCTCTA CCCACATCTT CCGTAAGATT ACGGACGCCA 
TCACCAAAAC CCATCACCTT GGACTCAAAT GTAGAGTCAG GATTTAGGGC AATATCCAGC 
CCTGGGGTAG GAAGTCAACC CCCAAGGTCT TGTCCATCCC CATTCAGATC CAACAGTCTT 
CGATCTAGTC CAGCAACAGT CCAGTCCCCT CCTCCTTATC GTAGCCAAAG GTCAATATCT 
CCAGCACCTC CAGTTAGCCT CCATTCAACT CCATCTGTTA ACAGCCCAAC CTCCACTGCT 
CTTGAACAAC CTTCGTTCAG CAGTTTAAAT TGTAATAATA ACAATAATAA CAGCTTAAGT 
AATAATGGGT GGGTCGCTAA TAACAAAAAA ACAGCACCAT CAGCTGTAAG TGGACTCCCT 
CATTTCCATG ATTCCCAGCG AACAACCTCA CACAATAGGG TGGCTCGTCC TTTCCCATCG 
TCTGAGCCTA GTTCCCGTGT ACAGTCCCCT TCCATTTGCC TCCAATCTCC AATCACCCGA 
ATCTGCTCTC CTCCACCTGC TCCCAACCAT TCCAGTCATC TGGTAACAAA GCCACCCAAC 
CCCAGAGCAT CCAGACAAGG TGGCTACTTT ACACCTTTAT CTTTGGAAAT TTCAAGATCA 
ACATCAGCTT GTTCAATATC TCCTTGCGGG AGTCCCAGAA TCACCTCTCC TCCCCCTATT 
GGCATTCCTA CAAATGTTTG GGGCTATGCT AATCCACAAC CAAGGAATCC ATTAGTAACA 
AGTGCATCGT CTCATACAAA ATTGGAAACA AACTCTACTC CAAGAGGCTC TAGAATCATC 
TCGCCCACCC CAACAGGATC ATCATCCTAT TCAGCCAACT CTCAGAGTCA GAGGAGGTTA 
CGAGGAACCA GTTTGCCCTT TGTTGTGATA GGGGATAGCC TTCCAAGTCC AACTAGACAC 
GAACGCAGAT CTTGGGCTGA AAGTGGACGG TGGTCAGTGG GTTCAGATCT AGGTTTAATA 
AGTCCTCGTG GGGGTTCATA CAGTGGTAGC CCGTCTTCTG TTAGCCCTGG TCCTCTTTCA 
CCAGTCAGAC TAACAGCCGA AAGGACAAGT GGAAAACACT TTACAGGCAT TTCCTGGCCA 
GATGTACACG ATCTCTTGAT TAAATACACA GAGCCCAATA CTGAAGCAAA TGCTTTATCC 
TGCACCGAGA TAGAGCCAGG TGACATGGAC GGTACAGAAC CTACATGTCG GAGTACCCTT 
ATTTGTCCAT ATGTTGCTCC AGCCAGCCCC ATTGAGAGAG ACACTCCCAT TCAGTGCCAG 
ACCGAGGCTA AAACAGAAGA CTGTGTTCCT AATCAAGGGT CTAAAACAAC ATTAAAGACC 
AGTTATGCCA CCACTGTCAA TTTGCAGATT GCTGGGAGTG GGAGGATTGC ATCCTTTAGC 
AATGCACAAG TCAGCCTGAC TCAAACTCTG ACCCCTGTGG CAGACAGTCA GAGCAGGAGA 
AGAGTCAGCA TCAACAGCTG CAACTTCTCT ATGCAGAACT GCAAAAGGCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PLEKHG2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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