tut1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tut1
Entrez Gene ID	564388
Full Name	terminal uridylyl transferase 1, U6 snRNA-specific
Synonyms	zgc:112254
General protein information	Preferred Names terminal uridylyl transferase 1, U6 snRNA-specific Names speckle targeted PIP5K1A-regulated poly(A) polymerase RNA-binding motif protein 21 RNA-binding protein 21 U6 snRNA-specific terminal uridylyltransferase 1 U6-TUTase star-PAP
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001030188.1	NP_001025359.1	speckle targeted PIP5K1A-regulated poly(A) polymerase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TUT1	XP_005631681.1
Rattus norvegicus (Norway rat)	Tut1	NP_001029073.1
Homo sapiens (human)	TUT1	NP_073741.2
Xenopus tropicalis (tropical clawed frog)	tut1	XP_002941502.2
Bos taurus (cattle)	TUT1	NP_001073791.1
Danio rerio (zebrafish)	tut1	NP_001025359.1
Mus musculus (house mouse)	Tut1	NP_932110.1
Pan troglodytes (chimpanzee)	TUT1	XP_508491.3

Gene Ontology
Bibliography

Related articles in PubMed

Terminal Uridylyltransferases Execute Programmed Clearance of Maternal Transcriptome in Vertebrate Embryos.
Chang H, Yeo J, Kim JG, Kim H, Lim J, Lee M, Kim HH, Ohk J, Jeon HY, Lee H, Jung H, Kim KW, Kim VN
Molecular cell70(1)72-82.e7(2018 04)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Hen1 is required for oocyte development and piRNA stability in zebrafish.
Kamminga LM, Luteijn MJ, den Broeder MJ, Redl S, Kaaij LJ, Roovers EF, Ladurner P, Berezikov E, Ketting RF
The EMBO journal29(21)3688-700(2010 Nov)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tut1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tut1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38220	NM_001030188.1 Latest version!	Danio rerio terminal uridylyl transferase 1, U6 snRNA-specific (tut1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tut1 ( NM_001030188.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001025359.1 Danio rerio terminal uridylyl transferase 1, U6 snRNA-specific (tut1), mRNA.

Price & Availability↑

CloneID	ODa38220
Clone ID Related Accession (Same CDS sequence)	NM_001030188.1
Accession Version	NM_001030188.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1547740800000
Organism	Danio rerio(zebrafish)
Product	speckle targeted PIP5K1A-regulated poly(A) polymerase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC098614.1. On Aug 6, 2005 this sequence version replaced XM_687726.1. ##Evidence-Data-START## Transcript exon combination :: BC098614.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCTCG ACAAAGACAT CCAGACAACT CAAAAGGGAT TTCATTGCAA CCTCTGCCAT 
GTTAACATAC CTAACAGGCC CAGTTTGGAA GATCATGTTA AAGGAAAGAA ACACCTGCAT 
CTCCTGCGCC TCAGAGCTCA GCGGAAAACT CAGGAAGAAA ACAGTGTGTT TGTGAGCGGC 
TTTAAAGCAG ACACGTCTCA GACTGAGCTG AAGGAGTATT TCCAACAGTT TGGACTGGTG 
ACGGATGTGA TCATGGACAA ACAGAAGGGT GTGTATGCCA TAGTTGAGTT TTCAGAGTCT 
CAGGATGTTC AGACAACTCT GGCACAACCA CAGCATCAGC TAAATGGACT CAAGCTCCGT 
GTCAAACCCA GAGAGAAGAA AGAGTTCAAG CTGGCCAGCA GAGGGAAACA AGACTGCAAA 
AACACCCTGA TCAGCCTGGA CAAACTCAAC TTTGAGCTCT GCAAGGCTAT GTCTGTAAAT 
GAGCAGATAC AGAAAGTAGT CGAGAGTTTG GAGTTGAAAG ATAACGAGAA GAAAGTGAGA 
GATCTTCTAG TTCAGCTGCT GCAGGAAGTT TTCACAGAGT TCTTTCCAGA TTGCCAGATT 
GTGCCCTTTG GCTCGTCTGT GAACACCTTT GGGCTTCACT CCTGTGATCT TGATCTGTTC 
CTTGACCTTG AGAACACCAA AGTATTTCAA GCTCGTGCAA AGTCTTCAGA ACAGACAGGA 
GAGAACCAGT CCGAGGATTG CCGTTCTGAA GACTCCATTT TGTCTGACAT TGACCTGTCC 
ACTGCCTCTC CTGCTGAAAT TTTGGAGCTG GTTGCTGTTA TTTTGAGGAA GTGCGTTCCT 
GGTGTTCACA AAGTACAGGC GCTCAGCACT GCTCGACTTC CTGTGGTCAA GTTCAGTCAC 
AAGGAGCTCA ACCTCCAAGG AGACATCACC ATCAACAACA GATTGGCAGT AAGGAACACA 
AAATTTCTGC AGTTGTGTTC AGGTATCGAC TCCAGACTCC GACCCTTAGT CTACACCATC 
CGTTTATGGG CTAAGCAGAA ACAACTAGCA GGAAACCTGT CTGGCCCGGG TCCATTGTTA 
AACAATTACG CTCTCACGCT GCTGGTGATC TTCTTCCTTC AGAATCGAGA CCCTCCTGTG 
CTTCCTTCTG TCAATCAGCT CAAGAACATG GCCTGTGAGG AAGAAGAGTG TGCTATTGAG 
GAATGGGATT GCACATTTCC TAGCCAGCCC TTTAGTGTAC CGCCCAGCAA AAACACTGAA 
GATCTCTGCA CTTTGCTTTT TGGATTCTTC ACCTTTTACT CCAAGTTTGA TTTCCCTGCA 
TCAGTGGTGT CTCTGAGAGA TGGCCATGTG CTGCCTATCA CAGACTTCCT CAAAAGTGAC 
ATGGAAGCGT TAAAAACTGC AGATGCCTCT AGTCCCAAAC CAAAGCGTTC TTCAGCTCCA 
AGACTGGGCC CTATGAATGT CCTTGATCCA TTTGAGCTGA ACCACAATGT GGCAGGAAAC 
CTTAACGAGC GAACGCAGAA GAACTTCAAG AGGGAGTGCT GCGAGGCTGA GAAGTACTGT 
CGCAGTCTGC AGTACCAGCG CAAATCAGCC AAGGGCAAAT CCTGGGGCCT CGTGCGACTC 
TTTGCACCGC AGAGTGAAGC TGCAGCCTCT TCACAGCCCA GAGCTGAGAA GGTTTTGGAG 
GTCAGCGTCC CTTTCAAGCC AGCCAGTCTC CCAGAATCCT TGCGTGCCCA GCTGGCTTCG 
GCTGGGAAGG ATTTTCGGGG GCTCTGGTTT GCCGAGGTCT GCTCTGCTGT GCAGAAGGTG 
TTTAATGAAA TCTTACAGTG TTCACCCACA GAGGAGACTC AAAGTTTGGA CAAAACCGAT 
AAAAGTGGAA GTGAAATGGA GGTAAATAAT AACAGGAGTC TAGAAGATAC TAACATCCAA 
GTGAAAGGTG AGGCTGGTAA AAAGAGGCCT CTTTCAGTGG AGGAGGGTCC ATCAACTTTC 
ACAATTACAC AAGCGAAAAG ACAAAGACTA GATGTTGACC TGGAGCACCC GGAGCCACTC 
CACTGGACTT GGACTCAGAG GAGCCGTGTT TGGGCAGGCC GGCGGAAAGT GCGGAGAGAT 
CTCTTGAAGA CCAGCGATGA GGCATCCAAA CCTGAAGGAG GCTGTGTGGA TATGGAGAGC 
AGGGTGACGC AGAGCATTGT GGAGAAAGAG GAGAAACTTC ACGATGCGTT GGAGTTCAAA 
GTGGACGCTG AGGTCGTTGG AGGGAATGAA AGCACTAAGG TTGTCCTCCG TTTCCACCCG 
AGCATTGATA CTGCAGGCGT CTTTCAAGAT TTTTTCCATT TTCTGGAGTC CTTCCTGCCT 
AAGATGGCAG AGACGATCAT GGGCAGGGCA GAGGACATTA CAGATATGTC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025359.1
CDS	44..2437
Translation	MELDKDIQTTQKGFHCNLCHVNIPNRPSLEDHVKGKKHLHLLRLRAQRKTQEENSVFVSGFKADTSQTELKEYFQQFGLVTDVIMDKQKGVYAIVEFSESQDVQTTLAQPQHQLNGLKLRVKPREKKEFKLASRGKQDCKNTLISLDKLNFELCKAMSVNEQIQKVVESLELKDNEKKVRDLLVQLLQEVFTEFFPDCQIVPFGSSVNTFGLHSCDLDLFLDLENTKVFQARAKSSEQTGENQSEDCRSEDSILSDIDLSTASPAEILELVAVILRKCVPGVHKVQALSTARLPVVKFSHKELNLQGDITINNRLAVRNTKFLQLCSGIDSRLRPLVYTIRLWAKQKQLAGNLSGPGPLLNNYALTLLVIFFLQNRDPPVLPSVNQLKNMACEEEECAIEEWDCTFPSQPFSVPPSKNTEDLCTLLFGFFTFYSKFDFPASVVSLRDGHVLPITDFLKSDMEALKTADASSPKPKRSSAPRLGPMNVLDPFELNHNVAGNLNERTQKNFKRECCEAEKYCRSLQYQRKSAKGKSWGLVRLFAPQSEAAASSQPRAEKVLEVSVPFKPASLPESLRAQLASAGKDFRGLWFAEVCSAVQKVFNEILQCSPTEETQSLDKTDKSGSEMEVNNNRSLEDTNIQVKGEAGKKRPLSVEEGPSTFTITQAKRQRLDVDLEHPEPLHWTWTQRSRVWAGRRKVRRDLLKTSDEASKPEGGCVDMESRVTQSIVEKEEKLHDALEFKVDAEVVGGNESTKVVLRFHPSIDTAGVFQDFFHFLESFLPKMAETIMGRAEDITDMS

Target ORF information:

RefSeq Version	NM_001030188.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio terminal uridylyl transferase 1, U6 snRNA-specific (tut1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030188.1

ORF Insert Sequence:

ATGGAGCTCG ACAAAGACAT CCAGACAACT CAAAAGGGAT TTCATTGCAA CCTCTGCCAT 
GTTAACATAC CTAACAGGCC CAGTTTGGAA GATCATGTTA AAGGAAAGAA ACACCTGCAT 
CTCCTGCGCC TCAGAGCTCA GCGGAAAACT CAGGAAGAAA ACAGTGTGTT TGTGAGCGGC 
TTTAAAGCAG ACACGTCTCA GACTGAGCTG AAGGAGTATT TCCAACAGTT TGGACTGGTG 
ACGGATGTGA TCATGGACAA ACAGAAGGGT GTGTATGCCA TAGTTGAGTT TTCAGAGTCT 
CAGGATGTTC AGACAACTCT GGCACAACCA CAGCATCAGC TAAATGGACT CAAGCTCCGT 
GTCAAACCCA GAGAGAAGAA AGAGTTCAAG CTGGCCAGCA GAGGGAAACA AGACTGCAAA 
AACACCCTGA TCAGCCTGGA CAAACTCAAC TTTGAGCTCT GCAAGGCTAT GTCTGTAAAT 
GAGCAGATAC AGAAAGTAGT CGAGAGTTTG GAGTTGAAAG ATAACGAGAA GAAAGTGAGA 
GATCTTCTAG TTCAGCTGCT GCAGGAAGTT TTCACAGAGT TCTTTCCAGA TTGCCAGATT 
GTGCCCTTTG GCTCGTCTGT GAACACCTTT GGGCTTCACT CCTGTGATCT TGATCTGTTC 
CTTGACCTTG AGAACACCAA AGTATTTCAA GCTCGTGCAA AGTCTTCAGA ACAGACAGGA 
GAGAACCAGT CCGAGGATTG CCGTTCTGAA GACTCCATTT TGTCTGACAT TGACCTGTCC 
ACTGCCTCTC CTGCTGAAAT TTTGGAGCTG GTTGCTGTTA TTTTGAGGAA GTGCGTTCCT 
GGTGTTCACA AAGTACAGGC GCTCAGCACT GCTCGACTTC CTGTGGTCAA GTTCAGTCAC 
AAGGAGCTCA ACCTCCAAGG AGACATCACC ATCAACAACA GATTGGCAGT AAGGAACACA 
AAATTTCTGC AGTTGTGTTC AGGTATCGAC TCCAGACTCC GACCCTTAGT CTACACCATC 
CGTTTATGGG CTAAGCAGAA ACAACTAGCA GGAAACCTGT CTGGCCCGGG TCCATTGTTA 
AACAATTACG CTCTCACGCT GCTGGTGATC TTCTTCCTTC AGAATCGAGA CCCTCCTGTG 
CTTCCTTCTG TCAATCAGCT CAAGAACATG GCCTGTGAGG AAGAAGAGTG TGCTATTGAG 
GAATGGGATT GCACATTTCC TAGCCAGCCC TTTAGTGTAC CGCCCAGCAA AAACACTGAA 
GATCTCTGCA CTTTGCTTTT TGGATTCTTC ACCTTTTACT CCAAGTTTGA TTTCCCTGCA 
TCAGTGGTGT CTCTGAGAGA TGGCCATGTG CTGCCTATCA CAGACTTCCT CAAAAGTGAC 
ATGGAAGCGT TAAAAACTGC AGATGCCTCT AGTCCCAAAC CAAAGCGTTC TTCAGCTCCA 
AGACTGGGCC CTATGAATGT CCTTGATCCA TTTGAGCTGA ACCACAATGT GGCAGGAAAC 
CTTAACGAGC GAACGCAGAA GAACTTCAAG AGGGAGTGCT GCGAGGCTGA GAAGTACTGT 
CGCAGTCTGC AGTACCAGCG CAAATCAGCC AAGGGCAAAT CCTGGGGCCT CGTGCGACTC 
TTTGCACCGC AGAGTGAAGC TGCAGCCTCT TCACAGCCCA GAGCTGAGAA GGTTTTGGAG 
GTCAGCGTCC CTTTCAAGCC AGCCAGTCTC CCAGAATCCT TGCGTGCCCA GCTGGCTTCG 
GCTGGGAAGG ATTTTCGGGG GCTCTGGTTT GCCGAGGTCT GCTCTGCTGT GCAGAAGGTG 
TTTAATGAAA TCTTACAGTG TTCACCCACA GAGGAGACTC AAAGTTTGGA CAAAACCGAT 
AAAAGTGGAA GTGAAATGGA GGTAAATAAT AACAGGAGTC TAGAAGATAC TAACATCCAA 
GTGAAAGGTG AGGCTGGTAA AAAGAGGCCT CTTTCAGTGG AGGAGGGTCC ATCAACTTTC 
ACAATTACAC AAGCGAAAAG ACAAAGACTA GATGTTGACC TGGAGCACCC GGAGCCACTC 
CACTGGACTT GGACTCAGAG GAGCCGTGTT TGGGCAGGCC GGCGGAAAGT GCGGAGAGAT 
CTCTTGAAGA CCAGCGATGA GGCATCCAAA CCTGAAGGAG GCTGTGTGGA TATGGAGAGC 
AGGGTGACGC AGAGCATTGT GGAGAAAGAG GAGAAACTTC ACGATGCGTT GGAGTTCAAA 
GTGGACGCTG AGGTCGTTGG AGGGAATGAA AGCACTAAGG TTGTCCTCCG TTTCCACCCG 
AGCATTGATA CTGCAGGCGT CTTTCAAGAT TTTTTCCATT TTCTGGAGTC CTTCCTGCCT 
AAGATGGCAG AGACGATCAT GGGCAGGGCA GAGGACATTA CAGATATGTC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Terminal Uridylyltransferases Execute Programmed Clearance of Maternal Transcriptome in Vertebrate Embryos.
Molecular cell70(1)72-82.e7(2018 04)
Chang H,Yeo J,Kim JG,Kim H,Lim J,Lee M,Kim HH,Ohk J,Jeon HY,Lee H,Jung H,Kim KW,Kim VN

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Hen1 is required for oocyte development and piRNA stability in zebrafish.
The EMBO journal29(21)3688-700(2010 Nov)
Kamminga LM,Luteijn MJ,den Broeder MJ,Redl S,Kaaij LJ,Roovers EF,Ladurner P,Berezikov E,Ketting RF

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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tut1 cDNA ORF clone, Danio rerio(zebrafish)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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