glsa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	glsa
Entrez Gene ID	564147
Full Name	glutaminase a
Synonyms	glsl
General protein information	Preferred Names glutaminase a Names glutaminase a glutaminase, like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005161685.3	XP_005161742.1	glutaminase a isoform X1
XM_021469341.1	XP_021325016.1	glutaminase a isoform X3
XM_009296204.3	XP_009294479.1	glutaminase a isoform X2
XM_009296204.2	XP_009294479.1	glutaminase a isoform X2
NM_001045044.1	NP_001038509.1	glutaminase a
XM_005161685.4	XP_005161742.1	glutaminase a isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	glsa	NP_001038509.1
Bos taurus (cattle)	GLS	NP_001071432.1
Gallus gallus (chicken)	GLS	NP_001026419.1
Caenorhabditis elegans (roundworm)	glna-1	NP_497055.2
Canis lupus familiaris (dog)	GLS	XP_545570.2
Mus musculus (house mouse)	Gls	NP_001074550.1
Danio rerio (zebrafish)	glsb	XP_693171.3
Caenorhabditis elegans (roundworm)	glna-2	NP_495675.1
Homo sapiens (human)	GLS	NP_055720.3
Macaca mulatta (Rhesus monkey)	GLS	XP_002799041.1
Rattus norvegicus (Norway rat)	Gls	NP_036701.2
Xenopus tropicalis (tropical clawed frog)	gls	XP_002931560.2
Pan troglodytes (chimpanzee)	GLS	XP_001167502.1
Drosophila melanogaster (fruit fly)	CG42708	NP_725217.1
Caenorhabditis elegans (roundworm)	glna-3	NP_492162.1

Gene Ontology
Bibliography

Related articles in PubMed

QDPR homologues in Danio rerio regulate melanin synthesis, early gliogenesis, and glutamine homeostasis.
Breuer M, Guglielmi L, Zielonka M, Hemberger V, Kölker S, Okun JG, Hoffmann GF, Carl M, Sauer SW, Opladen T
PloS one14(4)e0215162(2019)

Glucocorticoid deficiency causes transcriptional and post-transcriptional reprogramming of glutamine metabolism.
Weger M, Weger BD, Görling B, Poschet G, Yildiz M, Hell R, Luy B, Akcay T, Güran T, Dickmeis T, Müller F, Krone N
EBioMedicine36376-389(2018 10)

Pharmacologic rescue of hyperammonemia-induced toxicity in zebrafish by inhibition of ornithine aminotransferase.
Zielonka M, Breuer M, Okun JG, Carl M, Hoffmann GF, Kölker S
PloS one13(9)e0203707(2018)

Microcystin-LR induces changes in the GABA neurotransmitter system of zebrafish.
Yan W, Li L, Li G, Zhao S
Aquatic toxicology (Amsterdam, Netherlands)188170-176(2017 Jul)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following glsa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the glsa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa151189	XM_021469341.1 Latest version!	Danio rerio glutaminase a (glsa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ODa36530	NM_001045044.1 Latest version!	Danio rerio glutaminase a (glsa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa26779	XM_005161685.4 Latest version!	Danio rerio glutaminase a (glsa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa44249	XM_009296204.3 Latest version!	Danio rerio glutaminase a (glsa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
ODa44249	XM_009296204.2	Danio rerio glutaminase a (glsa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa26779	XM_005161685.3	Danio rerio glutaminase a (glsa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

glsa ( XM_021469341.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325016.1 Danio rerio glutaminase a (glsa), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa151189
Clone ID Related Accession (Same CDS sequence)	XM_021469341.1
Accession Version	XM_021469341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	glutaminase a isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTTGC AAGGTGTAAG CAACGCAGAG AAGTTTGACT ATGTGTTGAA TTTCATGAAG 
AAGTTGGCAG GAAATGAATA TGTTGGTTTC AGCAATGCTA CTTTTCAGTC AGAGCGATTG 
TCAGGAGACA GAAACTTTGC CATTGGATAC TACTTGAAGG AGAAGAAGTG TTTCCCAGAG 
GGCACCGATA TGATCTCCAT CCTGGACCTT TACTTTCAGC TGTGTTCCAT TGAGGTGACC 
TGTGAAAGTG CCAGTGTTAT GGCAGCAACT CTGGCGAACG GCGGCTTCTG TCCAATCACA 
GGCGAGCGAG TGGTCAACCC CGAGGCTGTG CGAAACACTC TGAGCCTCAT GCACTCCTGC 
GGCATGTACG ACTTCTCTGG ACAGTTCGCT TTCCATGTTG GTCTGCCGGC TAAATCAGGT 
GTTTCTGGAG GCATCCTGCT GGTCGTGCCC AACGTGATGG GAATCATGTG CTGGTCTCCA 
CCTCTTGACA AACTGGGCAA CAGCGTGAGG GGAATCCAGT TTTGCACGGA TCTGGTCCAA 
CTGTTTAATT TCCACAACTA TGACAATTTG CGGCACTTTG CCAAGAAACT GGATCCACGC 
AGAGAGGGCG GAGACCAACG GGTCAAGTCT GTCATCAACT TGCTGTTTGC GGCCTACACA 
GGGGACGTGT CTGCTTTGAG AAGGTTTGCC TTGTCATCGA TGGATATGGA GCAGAGAGAT 
TATGACTCGA GAACTGCTCT TCATGTAGCT GCTGCTGAAG GCCATGCTGA GGTTGTGCGT 
TTTCTGCTAG AAGCCTGTAA AGTGAATCCA GTGCCCAAGG ACAGGTGGGG AAACACTCCG 
ATGGATGAAG CCATTCATTT TGGCCATCAC GATGTGGTAA CAATCCTGCA GGACTATCAC 
AACACCTACA GCCCGAAGGA GAGCAGCGCC GACAGCGAAA AAGAGACGGC AGAGAAAAAC 
CTTGACGGGA TGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325016.1
CDS	3248..4225
Translation	MTLQGVSNAEKFDYVLNFMKKLAGNEYVGFSNATFQSERLSGDRNFAIGYYLKEKKCFPEGTDMISILDLYFQLCSIEVTCESASVMAATLANGGFCPITGERVVNPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSGVSGGILLVVPNVMGIMCWSPPLDKLGNSVRGIQFCTDLVQLFNFHNYDNLRHFAKKLDPRREGGDQRVKSVINLLFAAYTGDVSALRRFALSSMDMEQRDYDSRTALHVAAAEGHAEVVRFLLEACKVNPVPKDRWGNTPMDEAIHFGHHDVVTILQDYHNTYSPKESSADSEKETAEKNLDGML

Target ORF information:

RefSeq Version	XM_021469341.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutaminase a (glsa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469341.1

ORF Insert Sequence:

ATGACGTTGC AAGGTGTAAG CAACGCAGAG AAGTTTGACT ATGTGTTGAA TTTCATGAAG 
AAGTTGGCAG GAAATGAATA TGTTGGTTTC AGCAATGCTA CTTTTCAGTC AGAGCGATTG 
TCAGGAGACA GAAACTTTGC CATTGGATAC TACTTGAAGG AGAAGAAGTG TTTCCCAGAG 
GGCACCGATA TGATCTCCAT CCTGGACCTT TACTTTCAGC TGTGTTCCAT TGAGGTGACC 
TGTGAAAGTG CCAGTGTTAT GGCAGCAACT CTGGCGAACG GCGGCTTCTG TCCAATCACA 
GGCGAGCGAG TGGTCAACCC CGAGGCTGTG CGAAACACTC TGAGCCTCAT GCACTCCTGC 
GGCATGTACG ACTTCTCTGG ACAGTTCGCT TTCCATGTTG GTCTGCCGGC TAAATCAGGT 
GTTTCTGGAG GCATCCTGCT GGTCGTGCCC AACGTGATGG GAATCATGTG CTGGTCTCCA 
CCTCTTGACA AACTGGGCAA CAGCGTGAGG GGAATCCAGT TTTGCACGGA TCTGGTCCAA 
CTGTTTAATT TCCACAACTA TGACAATTTG CGGCACTTTG CCAAGAAACT GGATCCACGC 
AGAGAGGGCG GAGACCAACG GGTCAAGTCT GTCATCAACT TGCTGTTTGC GGCCTACACA 
GGGGACGTGT CTGCTTTGAG AAGGTTTGCC TTGTCATCGA TGGATATGGA GCAGAGAGAT 
TATGACTCGA GAACTGCTCT TCATGTAGCT GCTGCTGAAG GCCATGCTGA GGTTGTGCGT 
TTTCTGCTAG AAGCCTGTAA AGTGAATCCA GTGCCCAAGG ACAGGTGGGG AAACACTCCG 
ATGGATGAAG CCATTCATTT TGGCCATCAC GATGTGGTAA CAATCCTGCA GGACTATCAC 
AACACCTACA GCCCGAAGGA GAGCAGCGCC GACAGCGAAA AAGAGACGGC AGAGAAAAAC 
CTTGACGGGA TGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

glsa ( NM_001045044.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038509.1 Danio rerio glutaminase a (glsa), mRNA.

Price & Availability↑

CloneID	ODa36530
Clone ID Related Accession (Same CDS sequence)	NM_001045044.1
Accession Version	NM_001045044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1567180800000
Organism	Danio rerio(zebrafish)
Product	glutaminase a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX548072.14. On or before Sep 1, 2006 this sequence version replaced XM_703413.1, XM_703414.1, XM_703415.1, XM_703416.1, XM_681633.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: GFIL01011538.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTTACACT TTAGGCTTTC GAGGGCGTTA AAGGAGATCT GCAAACCCAA TGTAAGGCGC 
GTTTGGAGAA ATAATACAGA TGAAACAACG GTCGCCGGAG GACGCGCATC TTCATCCAGA 
CGAGCTCTGT GCACGCAGAC AGATGCGGCC AATGACAAGA GAAGGAAGGC AGGCATTTTG 
CCCAGCCTGG AAGATCTACT GTTTTACACT ATCGCAGAAG GCCAGGAGAA AATTCCAGCT 
CACAAGTTTA CCACGGCGCT GAAAGCCACA GGTCTGCGAA CTGGAGACCC TCGTCTTAAG 
GAATGTATGG AGATGTTAAA GATCACCCTG AAAACCACTT CAGACGGTGC TCTGGATCGA 
CATCTCTTTA AAAAGTGTGT GCAAAGTAAC ATCGTGCTGC TAACTCAGGC TTTCCGGAAG 
AAGTTTGTCA TTCCTGACTT TCAGTCGTTT ACCTCTCATA TTGATCAGAT TTATGAGAGT 
GCTAAATCAC TGTCAGGTGG CCAGGTAGCA GATTACATTC CTCAGCTAGC GAAATTTAGC 
CCTAACCTCT GGGCTGTTTC TCTCTGCACT GTAGACGGTC AAAGATACAC TGCAGGCGAC 
ACTAAAGTTC CATTTTGTCT GCAGTCGTGC GTGAAACCGC TAAAATACGC AATTGCGGTT 
CACGATCACG GCACCGAATA CGTGCACCGC TTTATAGGAA AGGAACCCAG CGGTCTTCGT 
TTCAACAAAC TCTTCCTTAA TGAAGATGAT AAACCCCACA ATCCCATGGT AAACGCTGGC 
GCTATAGTTT GTACCTCCCT ACTCAAGCAA GGTGTAAGCA ACGCAGAGAA GTTTGACTAT 
GTGTTGAATT TCATGAAGAA GTTGGCAGGA AATGAATATG TTGGTTTCAG CAATGCTACT 
TTTCAGTCAG AGCGATTGTC AGGAGACAGA AACTTTGCCA TTGGATACTA CTTGAAGGAG 
AAGAAGTGTT TCCCAGAGGG CACCGATATG ATCTCCATCC TGGACCTTTA CTTTCAGCTG 
TGTTCCATTG AGGTGACCTG TGAAAGTGCC AGTGTTATGG CAGCAACTCT GGCGAACGGC 
GGCTTCTGTC CAATCACAGG CGAGCGAGTG GTCAACCCCG AGGCTGTGCG AAACACTCTG 
AGCCTCATGC ACTCCTGCGG CATGTACGAC TTCTCTGGAC AGTTCGCTTT CCATGTTGGT 
CTGCCGGCTA AATCAGGTGT TTCTGGAGGC ATCCTGCTGG TCGTGCCCAA CGTGATGGGA 
ATCATGTGCT GGTCTCCACC TCTTGACAAA CTGGGCAACA GCGTGAGGGG AATCCAGTTT 
TGCACGGATC TGGTCCAACT GTTTAATTTC CACAACTATG ACAATTTGCG GCACTTTGCC 
AAGAAACTGG ATCCACGCAG AGAGGGCGGA GACCAACGGG TCAAGTCTGT CATCAACTTG 
CTGTTTGCGG CCTACACAGG GGACGTGTCT GCTTTGAGAA GGTTTGCCTT GTCATCGATG 
GATATGGAGC AGAGAGATTA TGACTCGAGA ACTGCTCTTC ATGTAGCTGC TGCTGAAGGC 
CATGCTGAGG TTGTGCGTTT TCTGCTAGAA GCCTGTAAAG TGAATCCAGT GCCCAAGGAC 
AGGTGGGGAA ACACTCCGAT GGATGAAGCC ATTCATTTTG GCCATCACGA TGTGGTAACA 
ATCCTGCAGG ACTATCACAA CACCTACAGC CCGAAGGAGA GCAGCGCCGA CAGCGAAAAA 
GAGACGGCAG AGAAAAACCT TGACGGGATG CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038509.1
CDS	84..1859
Translation	MLHFRLSRALKEICKPNVRRVWRNNTDETTVAGGRASSSRRALCTQTDAANDKRRKAGILPSLEDLLFYTIAEGQEKIPAHKFTTALKATGLRTGDPRLKECMEMLKITLKTTSDGALDRHLFKKCVQSNIVLLTQAFRKKFVIPDFQSFTSHIDQIYESAKSLSGGQVADYIPQLAKFSPNLWAVSLCTVDGQRYTAGDTKVPFCLQSCVKPLKYAIAVHDHGTEYVHRFIGKEPSGLRFNKLFLNEDDKPHNPMVNAGAIVCTSLLKQGVSNAEKFDYVLNFMKKLAGNEYVGFSNATFQSERLSGDRNFAIGYYLKEKKCFPEGTDMISILDLYFQLCSIEVTCESASVMAATLANGGFCPITGERVVNPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSGVSGGILLVVPNVMGIMCWSPPLDKLGNSVRGIQFCTDLVQLFNFHNYDNLRHFAKKLDPRREGGDQRVKSVINLLFAAYTGDVSALRRFALSSMDMEQRDYDSRTALHVAAAEGHAEVVRFLLEACKVNPVPKDRWGNTPMDEAIHFGHHDVVTILQDYHNTYSPKESSADSEKETAEKNLDGML

Target ORF information:

RefSeq Version	NM_001045044.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutaminase a (glsa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045044.1

ORF Insert Sequence:

ATGTTACACT TTAGGCTTTC GAGGGCGTTA AAGGAGATCT GCAAACCCAA TGTAAGGCGC 
GTTTGGAGAA ATAATACAGA TGAAACAACG GTCGCCGGAG GACGCGCATC TTCATCCAGA 
CGAGCTCTGT GCACGCAGAC AGATGCGGCC AATGACAAGA GAAGGAAGGC AGGCATTTTG 
CCCAGCCTGG AAGATCTACT GTTTTACACT ATCGCAGAAG GCCAGGAGAA AATTCCAGCT 
CACAAGTTTA CCACGGCGCT GAAAGCCACA GGTCTGCGAA CTGGAGACCC TCGTCTTAAG 
GAATGTATGG AGATGTTAAA GATCACCCTG AAAACCACTT CAGACGGTGC TCTGGATCGA 
CATCTCTTTA AAAAGTGTGT GCAAAGTAAC ATCGTGCTGC TAACTCAGGC TTTCCGGAAG 
AAGTTTGTCA TTCCTGACTT TCAGTCGTTT ACCTCTCATA TTGATCAGAT TTATGAGAGT 
GCTAAATCAC TGTCAGGTGG CCAGGTAGCA GATTACATTC CTCAGCTAGC GAAATTTAGC 
CCTAACCTCT GGGCTGTTTC TCTCTGCACT GTAGACGGTC AAAGATACAC TGCAGGCGAC 
ACTAAAGTTC CATTTTGTCT GCAGTCGTGC GTGAAACCGC TAAAATACGC AATTGCGGTT 
CACGATCACG GCACCGAATA CGTGCACCGC TTTATAGGAA AGGAACCCAG CGGTCTTCGT 
TTCAACAAAC TCTTCCTTAA TGAAGATGAT AAACCCCACA ATCCCATGGT AAACGCTGGC 
GCTATAGTTT GTACCTCCCT ACTCAAGCAA GGTGTAAGCA ACGCAGAGAA GTTTGACTAT 
GTGTTGAATT TCATGAAGAA GTTGGCAGGA AATGAATATG TTGGTTTCAG CAATGCTACT 
TTTCAGTCAG AGCGATTGTC AGGAGACAGA AACTTTGCCA TTGGATACTA CTTGAAGGAG 
AAGAAGTGTT TCCCAGAGGG CACCGATATG ATCTCCATCC TGGACCTTTA CTTTCAGCTG 
TGTTCCATTG AGGTGACCTG TGAAAGTGCC AGTGTTATGG CAGCAACTCT GGCGAACGGC 
GGCTTCTGTC CAATCACAGG CGAGCGAGTG GTCAACCCCG AGGCTGTGCG AAACACTCTG 
AGCCTCATGC ACTCCTGCGG CATGTACGAC TTCTCTGGAC AGTTCGCTTT CCATGTTGGT 
CTGCCGGCTA AATCAGGTGT TTCTGGAGGC ATCCTGCTGG TCGTGCCCAA CGTGATGGGA 
ATCATGTGCT GGTCTCCACC TCTTGACAAA CTGGGCAACA GCGTGAGGGG AATCCAGTTT 
TGCACGGATC TGGTCCAACT GTTTAATTTC CACAACTATG ACAATTTGCG GCACTTTGCC 
AAGAAACTGG ATCCACGCAG AGAGGGCGGA GACCAACGGG TCAAGTCTGT CATCAACTTG 
CTGTTTGCGG CCTACACAGG GGACGTGTCT GCTTTGAGAA GGTTTGCCTT GTCATCGATG 
GATATGGAGC AGAGAGATTA TGACTCGAGA ACTGCTCTTC ATGTAGCTGC TGCTGAAGGC 
CATGCTGAGG TTGTGCGTTT TCTGCTAGAA GCCTGTAAAG TGAATCCAGT GCCCAAGGAC 
AGGTGGGGAA ACACTCCGAT GGATGAAGCC ATTCATTTTG GCCATCACGA TGTGGTAACA 
ATCCTGCAGG ACTATCACAA CACCTACAGC CCGAAGGAGA GCAGCGCCGA CAGCGAAAAA 
GAGACGGCAG AGAAAAACCT TGACGGGATG CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

glsa ( XM_005161685.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161742.1 Danio rerio glutaminase a (glsa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26779
Clone ID Related Accession (Same CDS sequence)	XM_005161685.3 , XM_005161685.4
Accession Version	XM_005161685.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	glutaminase a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161685.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACACT TTAGGCTTTC GAGGGCGTTA AAGGAGATCT GCAAACCCAA TGTAAGGCGC 
GTTTGGAGAA ATAATACAGA TGAAACAACG GTCGCCGGAG GACGCGCATC TTCATCCAGA 
CGAGCTCTGT GCACGCAGAC AGATGCGGCC AATGACAAGA AGGCAGGCAT TTTGCCCAGC 
CTGGAAGATC TACTGTTTTA CACTATCGCA GAAGGCCAGG AGAAAATTCC AGCTCACAAG 
TTTACCACGG CGCTGAAAGC CACAGGTCTG CGAACTGGAG ACCCTCGTCT TAAGGAATGT 
ATGGAGATGT TAAAGATCAC CCTGAAAACC ACTTCAGACG GTGCTCTGGA TCGACATCTC 
TTTAAAAAGT GTGTGCAAAG TAACATCGTG CTGCTAACTC AGGCTTTCCG GAAGAAGTTT 
GTCATTCCTG ACTTTCAGTC GTTTACCTCT CATATTGATC AGATTTATGA GAGTGCTAAA 
TCACTGTCAG GTGGCCAGGT AGCAGATTAC ATTCCTCAGC TAGCGAAATT TAGCCCTAAC 
CTCTGGGCTG TTTCTCTCTG CACTGTAGAC GGTCAAAGAT ACACTGCAGG CGACACTAAA 
GTTCCATTTT GTCTGCAGTC GTGCGTGAAA CCGCTAAAAT ACGCAATTGC GGTTCACGAT 
CACGGCACCG AATACGTGCA CCGCTTTATA GGAAAGGAAC CCAGCGGTCT TCGTTTCAAC 
AAACTCTTCC TTAATGAAGA TGATAAACCC CACAATCCCA TGGTAAACGC TGGCGCTATA 
GTTTGTACCT CCCTACTCAA GCAAGGTGTA AGCAACGCAG AGAAGTTTGA CTATGTGTTG 
AATTTCATGA AGAAGTTGGC AGGAAATGAA TATGTTGGTT TCAGCAATGC TACTTTTCAG 
TCAGAGCGAT TGTCAGGAGA CAGAAACTTT GCCATTGGAT ACTACTTGAA GGAGAAGAAG 
TGTTTCCCAG AGGGCACCGA TATGATCTCC ATCCTGGACC TTTACTTTCA GCTGTGTTCC 
ATTGAGGTGA CCTGTGAAAG TGCCAGTGTT ATGGCAGCAA CTCTGGCGAA CGGCGGCTTC 
TGTCCAATCA CAGGCGAGCG AGTGGTCAAC CCCGAGGCTG TGCGAAACAC TCTGAGCCTC 
ATGCACTCCT GCGGCATGTA CGACTTCTCT GGACAGTTCG CTTTCCATGT TGGTCTGCCG 
GCTAAATCAG GTGTTTCTGG AGGCATCCTG CTGGTCGTGC CCAACGTGAT GGGAATCATG 
TGCTGGTCTC CACCTCTTGA CAAACTGGGC AACAGCGTGA GGGGAATCCA GTTTTGCACG 
GATCTGGTCC AACTGTTTAA TTTCCACAAC TATGACAATT TGCGGCACTT TGCCAAGAAA 
CTGGATCCAC GCAGAGAGGG CGGAGACCAA CGGGTCAAGT CTGTCATCAA CTTGCTGTTT 
GCGGCCTACA CAGGGGACGT GTCTGCTTTG AGAAGGTTTG CCTTGTCATC GATGGATATG 
GAGCAGAGAG ATTATGACTC GAGAACTGCT CTTCATGTAG CTGCTGCTGA AGGCCATGCT 
GAGGTTGTGC GTTTTCTGCT AGAAGCCTGT AAAGTGAATC CAGTGCCCAA GGACAGGTGG 
GGAAACACTC CGATGGATGA AGCCATTCAT TTTGGCCATC ACGATGTGGT AACAATCCTG 
CAGGACTATC ACAACACCTA CAGCCCGAAG GAGAGCAGCG CCGACAGCGA AAAAGAGACG 
GCAGAGAAAA ACCTTGACGG GATGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161742.1
CDS	137..1906
Translation	MLHFRLSRALKEICKPNVRRVWRNNTDETTVAGGRASSSRRALCTQTDAANDKKAGILPSLEDLLFYTIAEGQEKIPAHKFTTALKATGLRTGDPRLKECMEMLKITLKTTSDGALDRHLFKKCVQSNIVLLTQAFRKKFVIPDFQSFTSHIDQIYESAKSLSGGQVADYIPQLAKFSPNLWAVSLCTVDGQRYTAGDTKVPFCLQSCVKPLKYAIAVHDHGTEYVHRFIGKEPSGLRFNKLFLNEDDKPHNPMVNAGAIVCTSLLKQGVSNAEKFDYVLNFMKKLAGNEYVGFSNATFQSERLSGDRNFAIGYYLKEKKCFPEGTDMISILDLYFQLCSIEVTCESASVMAATLANGGFCPITGERVVNPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSGVSGGILLVVPNVMGIMCWSPPLDKLGNSVRGIQFCTDLVQLFNFHNYDNLRHFAKKLDPRREGGDQRVKSVINLLFAAYTGDVSALRRFALSSMDMEQRDYDSRTALHVAAAEGHAEVVRFLLEACKVNPVPKDRWGNTPMDEAIHFGHHDVVTILQDYHNTYSPKESSADSEKETAEKNLDGML

Target ORF information:

RefSeq Version	XM_005161685.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glutaminase a (glsa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161685.3

ORF Insert Sequence:

ATGTTACACT TTAGGCTTTC GAGGGCGTTA AAGGAGATCT GCAAACCCAA TGTAAGGCGC 
GTTTGGAGAA ATAATACAGA TGAAACAACG GTCGCCGGAG GACGCGCATC TTCATCCAGA 
CGAGCTCTGT GCACGCAGAC AGATGCGGCC AATGACAAGA AGGCAGGCAT TTTGCCCAGC 
CTGGAAGATC TACTGTTTTA CACTATCGCA GAAGGCCAGG AGAAAATTCC AGCTCACAAG 
TTTACCACGG CGCTGAAAGC CACAGGTCTG CGAACTGGAG ACCCTCGTCT TAAGGAATGT 
ATGGAGATGT TAAAGATCAC CCTGAAAACC ACTTCAGACG GTGCTCTGGA TCGACATCTC 
TTTAAAAAGT GTGTGCAAAG TAACATCGTG CTGCTAACTC AGGCTTTCCG GAAGAAGTTT 
GTCATTCCTG ACTTTCAGTC GTTTACCTCT CATATTGATC AGATTTATGA GAGTGCTAAA 
TCACTGTCAG GTGGCCAGGT AGCAGATTAC ATTCCTCAGC TAGCGAAATT TAGCCCTAAC 
CTCTGGGCTG TTTCTCTCTG CACTGTAGAC GGTCAAAGAT ACACTGCAGG CGACACTAAA 
GTTCCATTTT GTCTGCAGTC GTGCGTGAAA CCGCTAAAAT ACGCAATTGC GGTTCACGAT 
CACGGCACCG AATACGTGCA CCGCTTTATA GGAAAGGAAC CCAGCGGTCT TCGTTTCAAC 
AAACTCTTCC TTAATGAAGA TGATAAACCC CACAATCCCA TGGTAAACGC TGGCGCTATA 
GTTTGTACCT CCCTACTCAA GCAAGGTGTA AGCAACGCAG AGAAGTTTGA CTATGTGTTG 
AATTTCATGA AGAAGTTGGC AGGAAATGAA TATGTTGGTT TCAGCAATGC TACTTTTCAG 
TCAGAGCGAT TGTCAGGAGA CAGAAACTTT GCCATTGGAT ACTACTTGAA GGAGAAGAAG 
TGTTTCCCAG AGGGCACCGA TATGATCTCC ATCCTGGACC TTTACTTTCA GCTGTGTTCC 
ATTGAGGTGA CCTGTGAAAG TGCCAGTGTT ATGGCAGCAA CTCTGGCGAA CGGCGGCTTC 
TGTCCAATCA CAGGCGAGCG AGTGGTCAAC CCCGAGGCTG TGCGAAACAC TCTGAGCCTC 
ATGCACTCCT GCGGCATGTA CGACTTCTCT GGACAGTTCG CTTTCCATGT TGGTCTGCCG 
GCTAAATCAG GTGTTTCTGG AGGCATCCTG CTGGTCGTGC CCAACGTGAT GGGAATCATG 
TGCTGGTCTC CACCTCTTGA CAAACTGGGC AACAGCGTGA GGGGAATCCA GTTTTGCACG 
GATCTGGTCC AACTGTTTAA TTTCCACAAC TATGACAATT TGCGGCACTT TGCCAAGAAA 
CTGGATCCAC GCAGAGAGGG CGGAGACCAA CGGGTCAAGT CTGTCATCAA CTTGCTGTTT 
GCGGCCTACA CAGGGGACGT GTCTGCTTTG AGAAGGTTTG CCTTGTCATC GATGGATATG 
GAGCAGAGAG ATTATGACTC GAGAACTGCT CTTCATGTAG CTGCTGCTGA AGGCCATGCT 
GAGGTTGTGC GTTTTCTGCT AGAAGCCTGT AAAGTGAATC CAGTGCCCAA GGACAGGTGG 
GGAAACACTC CGATGGATGA AGCCATTCAT TTTGGCCATC ACGATGTGGT AACAATCCTG 
CAGGACTATC ACAACACCTA CAGCCCGAAG GAGAGCAGCG CCGACAGCGA AAAAGAGACG 
GCAGAGAAAA ACCTTGACGG GATGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

glsa ( XM_005161685.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161742.1 Danio rerio glutaminase a (glsa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26779
Clone ID Related Accession (Same CDS sequence)	XM_005161685.3 , XM_005161685.4
Accession Version	XM_005161685.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	glutaminase a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161685.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACACT TTAGGCTTTC GAGGGCGTTA AAGGAGATCT GCAAACCCAA TGTAAGGCGC 
GTTTGGAGAA ATAATACAGA TGAAACAACG GTCGCCGGAG GACGCGCATC TTCATCCAGA 
CGAGCTCTGT GCACGCAGAC AGATGCGGCC AATGACAAGA AGGCAGGCAT TTTGCCCAGC 
CTGGAAGATC TACTGTTTTA CACTATCGCA GAAGGCCAGG AGAAAATTCC AGCTCACAAG 
TTTACCACGG CGCTGAAAGC CACAGGTCTG CGAACTGGAG ACCCTCGTCT TAAGGAATGT 
ATGGAGATGT TAAAGATCAC CCTGAAAACC ACTTCAGACG GTGCTCTGGA TCGACATCTC 
TTTAAAAAGT GTGTGCAAAG TAACATCGTG CTGCTAACTC AGGCTTTCCG GAAGAAGTTT 
GTCATTCCTG ACTTTCAGTC GTTTACCTCT CATATTGATC AGATTTATGA GAGTGCTAAA 
TCACTGTCAG GTGGCCAGGT AGCAGATTAC ATTCCTCAGC TAGCGAAATT TAGCCCTAAC 
CTCTGGGCTG TTTCTCTCTG CACTGTAGAC GGTCAAAGAT ACACTGCAGG CGACACTAAA 
GTTCCATTTT GTCTGCAGTC GTGCGTGAAA CCGCTAAAAT ACGCAATTGC GGTTCACGAT 
CACGGCACCG AATACGTGCA CCGCTTTATA GGAAAGGAAC CCAGCGGTCT TCGTTTCAAC 
AAACTCTTCC TTAATGAAGA TGATAAACCC CACAATCCCA TGGTAAACGC TGGCGCTATA 
GTTTGTACCT CCCTACTCAA GCAAGGTGTA AGCAACGCAG AGAAGTTTGA CTATGTGTTG 
AATTTCATGA AGAAGTTGGC AGGAAATGAA TATGTTGGTT TCAGCAATGC TACTTTTCAG 
TCAGAGCGAT TGTCAGGAGA CAGAAACTTT GCCATTGGAT ACTACTTGAA GGAGAAGAAG 
TGTTTCCCAG AGGGCACCGA TATGATCTCC ATCCTGGACC TTTACTTTCA GCTGTGTTCC 
ATTGAGGTGA CCTGTGAAAG TGCCAGTGTT ATGGCAGCAA CTCTGGCGAA CGGCGGCTTC 
TGTCCAATCA CAGGCGAGCG AGTGGTCAAC CCCGAGGCTG TGCGAAACAC TCTGAGCCTC 
ATGCACTCCT GCGGCATGTA CGACTTCTCT GGACAGTTCG CTTTCCATGT TGGTCTGCCG 
GCTAAATCAG GTGTTTCTGG AGGCATCCTG CTGGTCGTGC CCAACGTGAT GGGAATCATG 
TGCTGGTCTC CACCTCTTGA CAAACTGGGC AACAGCGTGA GGGGAATCCA GTTTTGCACG 
GATCTGGTCC AACTGTTTAA TTTCCACAAC TATGACAATT TGCGGCACTT TGCCAAGAAA 
CTGGATCCAC GCAGAGAGGG CGGAGACCAA CGGGTCAAGT CTGTCATCAA CTTGCTGTTT 
GCGGCCTACA CAGGGGACGT GTCTGCTTTG AGAAGGTTTG CCTTGTCATC GATGGATATG 
GAGCAGAGAG ATTATGACTC GAGAACTGCT CTTCATGTAG CTGCTGCTGA AGGCCATGCT 
GAGGTTGTGC GTTTTCTGCT AGAAGCCTGT AAAGTGAATC CAGTGCCCAA GGACAGGTGG 
GGAAACACTC CGATGGATGA AGCCATTCAT TTTGGCCATC ACGATGTGGT AACAATCCTG 
CAGGACTATC ACAACACCTA CAGCCCGAAG GAGAGCAGCG CCGACAGCGA AAAAGAGACG 
GCAGAGAAAA ACCTTGACGG GATGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161742.1
CDS	152..1921
Translation	MLHFRLSRALKEICKPNVRRVWRNNTDETTVAGGRASSSRRALCTQTDAANDKKAGILPSLEDLLFYTIAEGQEKIPAHKFTTALKATGLRTGDPRLKECMEMLKITLKTTSDGALDRHLFKKCVQSNIVLLTQAFRKKFVIPDFQSFTSHIDQIYESAKSLSGGQVADYIPQLAKFSPNLWAVSLCTVDGQRYTAGDTKVPFCLQSCVKPLKYAIAVHDHGTEYVHRFIGKEPSGLRFNKLFLNEDDKPHNPMVNAGAIVCTSLLKQGVSNAEKFDYVLNFMKKLAGNEYVGFSNATFQSERLSGDRNFAIGYYLKEKKCFPEGTDMISILDLYFQLCSIEVTCESASVMAATLANGGFCPITGERVVNPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSGVSGGILLVVPNVMGIMCWSPPLDKLGNSVRGIQFCTDLVQLFNFHNYDNLRHFAKKLDPRREGGDQRVKSVINLLFAAYTGDVSALRRFALSSMDMEQRDYDSRTALHVAAAEGHAEVVRFLLEACKVNPVPKDRWGNTPMDEAIHFGHHDVVTILQDYHNTYSPKESSADSEKETAEKNLDGML

Target ORF information:

RefSeq Version	XM_005161685.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutaminase a (glsa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161685.4

ORF Insert Sequence:

ATGTTACACT TTAGGCTTTC GAGGGCGTTA AAGGAGATCT GCAAACCCAA TGTAAGGCGC 
GTTTGGAGAA ATAATACAGA TGAAACAACG GTCGCCGGAG GACGCGCATC TTCATCCAGA 
CGAGCTCTGT GCACGCAGAC AGATGCGGCC AATGACAAGA AGGCAGGCAT TTTGCCCAGC 
CTGGAAGATC TACTGTTTTA CACTATCGCA GAAGGCCAGG AGAAAATTCC AGCTCACAAG 
TTTACCACGG CGCTGAAAGC CACAGGTCTG CGAACTGGAG ACCCTCGTCT TAAGGAATGT 
ATGGAGATGT TAAAGATCAC CCTGAAAACC ACTTCAGACG GTGCTCTGGA TCGACATCTC 
TTTAAAAAGT GTGTGCAAAG TAACATCGTG CTGCTAACTC AGGCTTTCCG GAAGAAGTTT 
GTCATTCCTG ACTTTCAGTC GTTTACCTCT CATATTGATC AGATTTATGA GAGTGCTAAA 
TCACTGTCAG GTGGCCAGGT AGCAGATTAC ATTCCTCAGC TAGCGAAATT TAGCCCTAAC 
CTCTGGGCTG TTTCTCTCTG CACTGTAGAC GGTCAAAGAT ACACTGCAGG CGACACTAAA 
GTTCCATTTT GTCTGCAGTC GTGCGTGAAA CCGCTAAAAT ACGCAATTGC GGTTCACGAT 
CACGGCACCG AATACGTGCA CCGCTTTATA GGAAAGGAAC CCAGCGGTCT TCGTTTCAAC 
AAACTCTTCC TTAATGAAGA TGATAAACCC CACAATCCCA TGGTAAACGC TGGCGCTATA 
GTTTGTACCT CCCTACTCAA GCAAGGTGTA AGCAACGCAG AGAAGTTTGA CTATGTGTTG 
AATTTCATGA AGAAGTTGGC AGGAAATGAA TATGTTGGTT TCAGCAATGC TACTTTTCAG 
TCAGAGCGAT TGTCAGGAGA CAGAAACTTT GCCATTGGAT ACTACTTGAA GGAGAAGAAG 
TGTTTCCCAG AGGGCACCGA TATGATCTCC ATCCTGGACC TTTACTTTCA GCTGTGTTCC 
ATTGAGGTGA CCTGTGAAAG TGCCAGTGTT ATGGCAGCAA CTCTGGCGAA CGGCGGCTTC 
TGTCCAATCA CAGGCGAGCG AGTGGTCAAC CCCGAGGCTG TGCGAAACAC TCTGAGCCTC 
ATGCACTCCT GCGGCATGTA CGACTTCTCT GGACAGTTCG CTTTCCATGT TGGTCTGCCG 
GCTAAATCAG GTGTTTCTGG AGGCATCCTG CTGGTCGTGC CCAACGTGAT GGGAATCATG 
TGCTGGTCTC CACCTCTTGA CAAACTGGGC AACAGCGTGA GGGGAATCCA GTTTTGCACG 
GATCTGGTCC AACTGTTTAA TTTCCACAAC TATGACAATT TGCGGCACTT TGCCAAGAAA 
CTGGATCCAC GCAGAGAGGG CGGAGACCAA CGGGTCAAGT CTGTCATCAA CTTGCTGTTT 
GCGGCCTACA CAGGGGACGT GTCTGCTTTG AGAAGGTTTG CCTTGTCATC GATGGATATG 
GAGCAGAGAG ATTATGACTC GAGAACTGCT CTTCATGTAG CTGCTGCTGA AGGCCATGCT 
GAGGTTGTGC GTTTTCTGCT AGAAGCCTGT AAAGTGAATC CAGTGCCCAA GGACAGGTGG 
GGAAACACTC CGATGGATGA AGCCATTCAT TTTGGCCATC ACGATGTGGT AACAATCCTG 
CAGGACTATC ACAACACCTA CAGCCCGAAG GAGAGCAGCG CCGACAGCGA AAAAGAGACG 
GCAGAGAAAA ACCTTGACGG GATGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

glsa ( XM_009296204.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294479.1 Danio rerio glutaminase a (glsa), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa44249
Clone ID Related Accession (Same CDS sequence)	XM_009296204.3 , XM_009296204.2
Accession Version	XM_009296204.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	glutaminase a isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296204.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACACT TTAGGCTTTC GAGGGCGTTA AAGGAGATCT GCAAACCCAA TGTAAGGCGC 
GTTTGGAGAA ATAATACAGA TGAAACAACG GTCGCCGGAG GACGCGCATC TTCATCCAGA 
CGAGCTCTGT GCACGCAGAC AGATGCGGCC AATGACAAGA GAAGGAAGGC AGGCATTTTG 
CCCAGCCTGG AAGATCTACT GTTTTACACT ATCGCAGAAG GCCAGGAGAA AATTCCAGCT 
CACAAGTTTA CCACGGCGCT GAAAGCCACA GGTCTGCGAA CTGGAGACCC TCGTCTTAAG 
GAATGTATGG AGATGTTAAA GATCACCCTG AAAACCACTT CAGACGGTGC TCTGGATCGA 
CATCTCTTTA AAAAGTGTGT GCAAAGTAAC ATCGTGCTGC TAACTCAGGC TTTCCGGAAG 
AAGTTTGTCA TTCCTGACTT TCAGTCGTTT ACCTCTCATA TTGATCAGAT TTATGAGAGT 
GCTAAATCAC TGTCAGGTGG CCAGGTAGCA GATTACATTC CTCAGCTAGC GAAATTTAGC 
CCTAACCTCT GGGCTGTTTC TCTCTGCACT GTAGACGGTC AAAGATACAC TGCAGGCGAC 
ACTAAAGTTC CATTTTGTCT GCAGTCGTGC GTGAAACCGC TAAAATACGC AATTGCGGTT 
CACGATCACG GCACCGAATA CGTGCACCGC TTTATAGGAA AGGAACCCAG CGGTCTTCGT 
TTCAACAAAC TCTTCCTTAA TGAAGATGAT AAACCCCACA ATCCCATGGT AAACGCTGGC 
GCTATAGTTT GTACCTCCCT ACTCAAGCAA GGTGTAAGCA ACGCAGAGAA GTTTGACTAT 
GTGTTGAATT TCATGAAGAA GTTGGCAGGA AATGAATATG TTGGTTTCAG CAATGCTACT 
TTTCAGTCAG AGCGATTGTC AGGAGACAGA AACTTTGCCA TTGGATACTA CTTGAAGGAG 
AAGAAGTGTT TCCCAGAGGG CACCGATATG ATCTCCATCC TGGACCTTTA CTTTCAGCTG 
TGTTCCATTG AGGTGACCTG TGAAAGTGCC AGTGTTATGG CAGCAACTCT GGCGAACGGC 
GGCTTCTGTC CAATCACAGG CGAGCGAGTG GTCAACCCCG AGGCTGTGCG AAACACTCTG 
AGCCTCATGC ACTCCTGCGG CATGTACGAC TTCTCTGGAC AGTTCGCTTT CCATGTTGGT 
CTGCCGGCTA AATCAGGTGT TTCTGGAGGC ATCCTGCTGG TCGTGCCCAA CGTGATGGGA 
ATCATGTGCT GGTCTCCACC TCTTGACAAA CTGGGCAACA GCGTGAGGGG AATCCAGTTT 
TGCACGGATC TGGTCCAACT GTTTAATTTC CACAACTATG ACAATTTGCG GCACTTTGCC 
AAGAAACTGG ATCCACGCAG AGAGGGCGGA GACCAACGGC AGCAGCAGAG TCTCGAACCG 
CTGGACTATG CGAGCCTCCA GCAGGAACTA GCGCTCACTG ACAAAGTGTG GAAAAAAGTT 
TGTCCCGAAA CGGATGCGGA CACTTCCACC ACTGTAGTCT ATAGGATGGA GAGGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294479.1
CDS	153..1712
Translation	MLHFRLSRALKEICKPNVRRVWRNNTDETTVAGGRASSSRRALCTQTDAANDKRRKAGILPSLEDLLFYTIAEGQEKIPAHKFTTALKATGLRTGDPRLKECMEMLKITLKTTSDGALDRHLFKKCVQSNIVLLTQAFRKKFVIPDFQSFTSHIDQIYESAKSLSGGQVADYIPQLAKFSPNLWAVSLCTVDGQRYTAGDTKVPFCLQSCVKPLKYAIAVHDHGTEYVHRFIGKEPSGLRFNKLFLNEDDKPHNPMVNAGAIVCTSLLKQGVSNAEKFDYVLNFMKKLAGNEYVGFSNATFQSERLSGDRNFAIGYYLKEKKCFPEGTDMISILDLYFQLCSIEVTCESASVMAATLANGGFCPITGERVVNPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSGVSGGILLVVPNVMGIMCWSPPLDKLGNSVRGIQFCTDLVQLFNFHNYDNLRHFAKKLDPRREGGDQRQQQSLEPLDYASLQQELALTDKVWKKVCPETDADTSTTVVYRMERN

Target ORF information:

RefSeq Version	XM_009296204.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutaminase a (glsa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296204.3

ORF Insert Sequence:

ATGTTACACT TTAGGCTTTC GAGGGCGTTA AAGGAGATCT GCAAACCCAA TGTAAGGCGC 
GTTTGGAGAA ATAATACAGA TGAAACAACG GTCGCCGGAG GACGCGCATC TTCATCCAGA 
CGAGCTCTGT GCACGCAGAC AGATGCGGCC AATGACAAGA GAAGGAAGGC AGGCATTTTG 
CCCAGCCTGG AAGATCTACT GTTTTACACT ATCGCAGAAG GCCAGGAGAA AATTCCAGCT 
CACAAGTTTA CCACGGCGCT GAAAGCCACA GGTCTGCGAA CTGGAGACCC TCGTCTTAAG 
GAATGTATGG AGATGTTAAA GATCACCCTG AAAACCACTT CAGACGGTGC TCTGGATCGA 
CATCTCTTTA AAAAGTGTGT GCAAAGTAAC ATCGTGCTGC TAACTCAGGC TTTCCGGAAG 
AAGTTTGTCA TTCCTGACTT TCAGTCGTTT ACCTCTCATA TTGATCAGAT TTATGAGAGT 
GCTAAATCAC TGTCAGGTGG CCAGGTAGCA GATTACATTC CTCAGCTAGC GAAATTTAGC 
CCTAACCTCT GGGCTGTTTC TCTCTGCACT GTAGACGGTC AAAGATACAC TGCAGGCGAC 
ACTAAAGTTC CATTTTGTCT GCAGTCGTGC GTGAAACCGC TAAAATACGC AATTGCGGTT 
CACGATCACG GCACCGAATA CGTGCACCGC TTTATAGGAA AGGAACCCAG CGGTCTTCGT 
TTCAACAAAC TCTTCCTTAA TGAAGATGAT AAACCCCACA ATCCCATGGT AAACGCTGGC 
GCTATAGTTT GTACCTCCCT ACTCAAGCAA GGTGTAAGCA ACGCAGAGAA GTTTGACTAT 
GTGTTGAATT TCATGAAGAA GTTGGCAGGA AATGAATATG TTGGTTTCAG CAATGCTACT 
TTTCAGTCAG AGCGATTGTC AGGAGACAGA AACTTTGCCA TTGGATACTA CTTGAAGGAG 
AAGAAGTGTT TCCCAGAGGG CACCGATATG ATCTCCATCC TGGACCTTTA CTTTCAGCTG 
TGTTCCATTG AGGTGACCTG TGAAAGTGCC AGTGTTATGG CAGCAACTCT GGCGAACGGC 
GGCTTCTGTC CAATCACAGG CGAGCGAGTG GTCAACCCCG AGGCTGTGCG AAACACTCTG 
AGCCTCATGC ACTCCTGCGG CATGTACGAC TTCTCTGGAC AGTTCGCTTT CCATGTTGGT 
CTGCCGGCTA AATCAGGTGT TTCTGGAGGC ATCCTGCTGG TCGTGCCCAA CGTGATGGGA 
ATCATGTGCT GGTCTCCACC TCTTGACAAA CTGGGCAACA GCGTGAGGGG AATCCAGTTT 
TGCACGGATC TGGTCCAACT GTTTAATTTC CACAACTATG ACAATTTGCG GCACTTTGCC 
AAGAAACTGG ATCCACGCAG AGAGGGCGGA GACCAACGGC AGCAGCAGAG TCTCGAACCG 
CTGGACTATG CGAGCCTCCA GCAGGAACTA GCGCTCACTG ACAAAGTGTG GAAAAAAGTT 
TGTCCCGAAA CGGATGCGGA CACTTCCACC ACTGTAGTCT ATAGGATGGA GAGGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

glsa ( XM_009296204.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294479.1 Danio rerio glutaminase a (glsa), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa44249
Clone ID Related Accession (Same CDS sequence)	XM_009296204.3 , XM_009296204.2
Accession Version	XM_009296204.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	glutaminase a isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296204.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACACT TTAGGCTTTC GAGGGCGTTA AAGGAGATCT GCAAACCCAA TGTAAGGCGC 
GTTTGGAGAA ATAATACAGA TGAAACAACG GTCGCCGGAG GACGCGCATC TTCATCCAGA 
CGAGCTCTGT GCACGCAGAC AGATGCGGCC AATGACAAGA GAAGGAAGGC AGGCATTTTG 
CCCAGCCTGG AAGATCTACT GTTTTACACT ATCGCAGAAG GCCAGGAGAA AATTCCAGCT 
CACAAGTTTA CCACGGCGCT GAAAGCCACA GGTCTGCGAA CTGGAGACCC TCGTCTTAAG 
GAATGTATGG AGATGTTAAA GATCACCCTG AAAACCACTT CAGACGGTGC TCTGGATCGA 
CATCTCTTTA AAAAGTGTGT GCAAAGTAAC ATCGTGCTGC TAACTCAGGC TTTCCGGAAG 
AAGTTTGTCA TTCCTGACTT TCAGTCGTTT ACCTCTCATA TTGATCAGAT TTATGAGAGT 
GCTAAATCAC TGTCAGGTGG CCAGGTAGCA GATTACATTC CTCAGCTAGC GAAATTTAGC 
CCTAACCTCT GGGCTGTTTC TCTCTGCACT GTAGACGGTC AAAGATACAC TGCAGGCGAC 
ACTAAAGTTC CATTTTGTCT GCAGTCGTGC GTGAAACCGC TAAAATACGC AATTGCGGTT 
CACGATCACG GCACCGAATA CGTGCACCGC TTTATAGGAA AGGAACCCAG CGGTCTTCGT 
TTCAACAAAC TCTTCCTTAA TGAAGATGAT AAACCCCACA ATCCCATGGT AAACGCTGGC 
GCTATAGTTT GTACCTCCCT ACTCAAGCAA GGTGTAAGCA ACGCAGAGAA GTTTGACTAT 
GTGTTGAATT TCATGAAGAA GTTGGCAGGA AATGAATATG TTGGTTTCAG CAATGCTACT 
TTTCAGTCAG AGCGATTGTC AGGAGACAGA AACTTTGCCA TTGGATACTA CTTGAAGGAG 
AAGAAGTGTT TCCCAGAGGG CACCGATATG ATCTCCATCC TGGACCTTTA CTTTCAGCTG 
TGTTCCATTG AGGTGACCTG TGAAAGTGCC AGTGTTATGG CAGCAACTCT GGCGAACGGC 
GGCTTCTGTC CAATCACAGG CGAGCGAGTG GTCAACCCCG AGGCTGTGCG AAACACTCTG 
AGCCTCATGC ACTCCTGCGG CATGTACGAC TTCTCTGGAC AGTTCGCTTT CCATGTTGGT 
CTGCCGGCTA AATCAGGTGT TTCTGGAGGC ATCCTGCTGG TCGTGCCCAA CGTGATGGGA 
ATCATGTGCT GGTCTCCACC TCTTGACAAA CTGGGCAACA GCGTGAGGGG AATCCAGTTT 
TGCACGGATC TGGTCCAACT GTTTAATTTC CACAACTATG ACAATTTGCG GCACTTTGCC 
AAGAAACTGG ATCCACGCAG AGAGGGCGGA GACCAACGGC AGCAGCAGAG TCTCGAACCG 
CTGGACTATG CGAGCCTCCA GCAGGAACTA GCGCTCACTG ACAAAGTGTG GAAAAAAGTT 
TGTCCCGAAA CGGATGCGGA CACTTCCACC ACTGTAGTCT ATAGGATGGA GAGGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294479.1
CDS	137..1696
Translation	MLHFRLSRALKEICKPNVRRVWRNNTDETTVAGGRASSSRRALCTQTDAANDKRRKAGILPSLEDLLFYTIAEGQEKIPAHKFTTALKATGLRTGDPRLKECMEMLKITLKTTSDGALDRHLFKKCVQSNIVLLTQAFRKKFVIPDFQSFTSHIDQIYESAKSLSGGQVADYIPQLAKFSPNLWAVSLCTVDGQRYTAGDTKVPFCLQSCVKPLKYAIAVHDHGTEYVHRFIGKEPSGLRFNKLFLNEDDKPHNPMVNAGAIVCTSLLKQGVSNAEKFDYVLNFMKKLAGNEYVGFSNATFQSERLSGDRNFAIGYYLKEKKCFPEGTDMISILDLYFQLCSIEVTCESASVMAATLANGGFCPITGERVVNPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSGVSGGILLVVPNVMGIMCWSPPLDKLGNSVRGIQFCTDLVQLFNFHNYDNLRHFAKKLDPRREGGDQRQQQSLEPLDYASLQQELALTDKVWKKVCPETDADTSTTVVYRMERN

Target ORF information:

RefSeq Version	XM_009296204.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glutaminase a (glsa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296204.2

ORF Insert Sequence:

ATGTTACACT TTAGGCTTTC GAGGGCGTTA AAGGAGATCT GCAAACCCAA TGTAAGGCGC 
GTTTGGAGAA ATAATACAGA TGAAACAACG GTCGCCGGAG GACGCGCATC TTCATCCAGA 
CGAGCTCTGT GCACGCAGAC AGATGCGGCC AATGACAAGA GAAGGAAGGC AGGCATTTTG 
CCCAGCCTGG AAGATCTACT GTTTTACACT ATCGCAGAAG GCCAGGAGAA AATTCCAGCT 
CACAAGTTTA CCACGGCGCT GAAAGCCACA GGTCTGCGAA CTGGAGACCC TCGTCTTAAG 
GAATGTATGG AGATGTTAAA GATCACCCTG AAAACCACTT CAGACGGTGC TCTGGATCGA 
CATCTCTTTA AAAAGTGTGT GCAAAGTAAC ATCGTGCTGC TAACTCAGGC TTTCCGGAAG 
AAGTTTGTCA TTCCTGACTT TCAGTCGTTT ACCTCTCATA TTGATCAGAT TTATGAGAGT 
GCTAAATCAC TGTCAGGTGG CCAGGTAGCA GATTACATTC CTCAGCTAGC GAAATTTAGC 
CCTAACCTCT GGGCTGTTTC TCTCTGCACT GTAGACGGTC AAAGATACAC TGCAGGCGAC 
ACTAAAGTTC CATTTTGTCT GCAGTCGTGC GTGAAACCGC TAAAATACGC AATTGCGGTT 
CACGATCACG GCACCGAATA CGTGCACCGC TTTATAGGAA AGGAACCCAG CGGTCTTCGT 
TTCAACAAAC TCTTCCTTAA TGAAGATGAT AAACCCCACA ATCCCATGGT AAACGCTGGC 
GCTATAGTTT GTACCTCCCT ACTCAAGCAA GGTGTAAGCA ACGCAGAGAA GTTTGACTAT 
GTGTTGAATT TCATGAAGAA GTTGGCAGGA AATGAATATG TTGGTTTCAG CAATGCTACT 
TTTCAGTCAG AGCGATTGTC AGGAGACAGA AACTTTGCCA TTGGATACTA CTTGAAGGAG 
AAGAAGTGTT TCCCAGAGGG CACCGATATG ATCTCCATCC TGGACCTTTA CTTTCAGCTG 
TGTTCCATTG AGGTGACCTG TGAAAGTGCC AGTGTTATGG CAGCAACTCT GGCGAACGGC 
GGCTTCTGTC CAATCACAGG CGAGCGAGTG GTCAACCCCG AGGCTGTGCG AAACACTCTG 
AGCCTCATGC ACTCCTGCGG CATGTACGAC TTCTCTGGAC AGTTCGCTTT CCATGTTGGT 
CTGCCGGCTA AATCAGGTGT TTCTGGAGGC ATCCTGCTGG TCGTGCCCAA CGTGATGGGA 
ATCATGTGCT GGTCTCCACC TCTTGACAAA CTGGGCAACA GCGTGAGGGG AATCCAGTTT 
TGCACGGATC TGGTCCAACT GTTTAATTTC CACAACTATG ACAATTTGCG GCACTTTGCC 
AAGAAACTGG ATCCACGCAG AGAGGGCGGA GACCAACGGC AGCAGCAGAG TCTCGAACCG 
CTGGACTATG CGAGCCTCCA GCAGGAACTA GCGCTCACTG ACAAAGTGTG GAAAAAAGTT 
TGTCCCGAAA CGGATGCGGA CACTTCCACC ACTGTAGTCT ATAGGATGGA GAGGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

QDPR homologues in Danio rerio regulate melanin synthesis, early gliogenesis, and glutamine homeostasis.
PloS one14(4)e0215162(2019)
Breuer M,Guglielmi L,Zielonka M,Hemberger V,Kölker S,Okun JG,Hoffmann GF,Carl M,Sauer SW,Opladen T

Glucocorticoid deficiency causes transcriptional and post-transcriptional reprogramming of glutamine metabolism.
EBioMedicine36376-389(2018 10)
Weger M,Weger BD,Görling B,Poschet G,Yildiz M,Hell R,Luy B,Akcay T,Güran T,Dickmeis T,Müller F,Krone N

Pharmacologic rescue of hyperammonemia-induced toxicity in zebrafish by inhibition of ornithine aminotransferase.
PloS one13(9)e0203707(2018)
Zielonka M,Breuer M,Okun JG,Carl M,Hoffmann GF,Kölker S

Microcystin-LR induces changes in the GABA neurotransmitter system of zebrafish.
Aquatic toxicology (Amsterdam, Netherlands)188170-176(2017 Jul)
Yan W,Li L,Li G,Zhao S

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

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glsa cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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