TBC1D31 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	TBC1D31
Entrez Gene ID	563134
Full Name	TBC1 domain family, member 31
Synonyms	wdr67
General protein information	Preferred Names TBC1 domain family, member 31 Names WD repeat-containing protein 67 TBC1 domain family member 31 WD repeat domain 67
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_689914.6	XP_695006.1	TBC1 domain family member 31
XM_689914.5	XP_695006.1	TBC1 domain family member 31

Pathways from BioSystems

Homologies

Homo sapiens (human)	TBC1D31	NP_663622.2
Gallus gallus (chicken)	WDR67	XP_004940076.1
Pan troglodytes (chimpanzee)	WDR67	XP_001146960.1
Bos taurus (cattle)	TBC1D31	NP_001039538.1
Xenopus tropicalis (tropical clawed frog)	tbc1d31	NP_001005061.1
Canis lupus familiaris (dog)	TBC1D31	XP_850449.1
Macaca mulatta (Rhesus monkey)	WDR67	XP_002805527.1
Mus musculus (house mouse)	Tbc1d31	NP_001074865.2
Rattus norvegicus (Norway rat)	Tbc1d31	NP_001128314.1
Danio rerio (zebrafish)	tbc1d31	XP_695006.1

Gene Ontology

The following TBC1D31 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TBC1D31 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa21494	XM_689914.6 Latest version!	Danio rerio TBC1 domain family, member 31 (tbc1d31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa21494	XM_689914.5	Danio rerio TBC1 domain family, member 31 (tbc1d31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TBC1D31 ( XM_689914.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_695006.1 Danio rerio TBC1 domain family, member 31 (tbc1d31), mRNA.

Price & Availability↑

CloneID	ODa21494
Clone ID Related Accession (Same CDS sequence)	XM_689914.6 , XM_689914.5
Accession Version	XM_689914.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	TBC1 domain family member 31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_689914.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTTA CAGATATCGG GTCAAGAGAA GACGGGAAAA TATGGCACCG AAAATCAGTA 
TCAAAAAACG GCGTTCTGGC CACTGTGATC CGCAACTCGT CTGCTCGTCA AACCAAGACG 
GTTCGATTCC TGCAAGTGGC TTTCGACACC ACCGGAGAGT CGTTTCTTGC TGGTGATCAT 
CATGGGAATA TATATGTATT TGACATCTGC AGAAACAGAT TCAAGCTTGT CCAGAAAACA 
GGACAGGCAT GCACTGCTTT AGCCTTCAAT TTAAGAAGAA CCACTGAGTT CCTGGTTGCT 
CTGGCTGATT ACTCCATCAA GTGCTTTGAC AAAGACACCA GGCAGCTCGT GAGCTGGATG 
CGGGGTCACG AAGGTGCCGT TTCCTCCTTG TCCATCCATA GCTCAGGCAG CTTCGCCATC 
AGCACGTCTT CTGACACCGC GCAGCTCTGG AGCCTCGACA CCTTCCAGAG AAAGAGGAAG 
CTCAATGTTC GTCAGTCGGT TGGCATACAA AAGGTGTTTT TTCTCCCACA CAGCAACACC 
ATTCTTAGCT GCTTTAGCGA CAATTCCATT TTTGCTTGGG AGACCGACAC TCTCTTCTGC 
AAATATCAAC TGCCTGTTCC ACAGAATGGG CCCAGAATTC ACTACAGGAC TTTTGCTGTT 
ACACAAAATG GAAAAACACT TGCTGCTGCT GGACGATCCA ACCTTGTCCA CTTGTGGTGT 
CTGGACAGTC AACAGCTCAT CAGAGTAATT CAGATGCCTT CAAATGTCAA GACAGTAAGA 
TATCTGGATT TCCTGCCAGA TAGTTTTGAT GACGGATCCA ACCAGACTCT AGGTGTTTTA 
AGCCAAGACG GCATCATGCG CTTTGTCAAC ATCCAGTCAT GCAAGCAGCT CTTTTTAATC 
GGATCCCACG AGGATGCAAT CAGCTCTGCA ACTGTTTGTC CTAAAGGTCA CCATATTGTC 
GCAGTGATGG ACAGTGGAGC TATTAAAATC TATGATGTGC GAAGTCTCAC CACAGAAATC 
AGTAAGCCTC CTCAGCCTTT GCTGAAACGG GTCACTAAAG TTAACATCAG TGATGGATCA 
TGTCTGAAAA TGAAGGTTAA CACTGGGGCA GGTCAGCGCG CTGTGAAAAC ATCAGGACGC 
AGAATCCAAT CCAAACTTTT ACCACCTTCA GTTTCTACTC TGGATGACAA AGAGAATGAT 
TTGCCTAATG GCCTAAGCAA GAAAAGACTA CAAGCGTTGC TAAGAGAGTT TGGCGAGTAT 
CCAGCAAAAT ACAGAATGTT TATTTGGCGT TCCCTGTTGC GTTTACCAGA GAACCATGCA 
GCTTTCAGCA GCTTGACTGA TAAAGGCCTG CATTCAGCCT TCCTGTGTCT TCAGGAGCAG 
TACCCCATCA AAAGCCACAA ACTACAAAGA GGCCTGCAGA GAGTGTTGTC TGCTCTTGCT 
CACTGGGCTG CAGTCTTCGG TGAGACTGAT TATGTCCCCC TGCTGGCCTT CCCATTCGTC 
AAGCTGTTCC AGAACAATCC TCTGATCTGC TTTGAAGCAG TGGCCACTGT CATTGTGAAC 
TGGTGCCAGC ACTGGTTTGA GTATTTTCCC AACCCTCCGC TTAATGTGCT GAGTCTGGCT 
GAGAATGTGC TGGCCCATCA TGATAAAGAG CTGCTTCAGC ATTTTGTTGC CTGTGGCGTT 
ACCTCACAGC TGTATGCGTG GCCTCTTCTA GAAACTCTGT TCTCTGAGGT TCTGATGAGA 
GAAGAGTGGC TGAAGCTATT TGACAATGTT TTCTCCAATC ATCCCTCATT CCTGCTAATG 
GCTGTGGTGG CGTATGTCAC CTGCTGCCGA GCACCGCTAC TGCTCTGCAC TGACAAGAGA 
GATTTTGAGT ACTTTTTCCA TCATCGCAAT AGTTTGGACA TTGGTGCTAT GCTGAAGGAA 
ACCTATCGGT TGATGGACAG AACCCCTGCC GACATCCATC CTAGCTCTGC GCTGAATGAT 
TTTGAACCTC TGACTCAGGG CCAGTATCCT GTCTTCAACA AATACCCCAC ATTCATAGTG 
GAGTACCAGT CTCAAGAACG CGAGAGGATC CGCCAACAAG AAGTGGAATA TCTGAGAGAG 
AGACAGGAGG TTCAGAAGCT GCATGCTGAG GCGGTCAGAC AGCAGGCTGA GTTTGAGGCC 
TGGTATAAAC AGCAGGAGCT GCTTCTGCAA GGAGAAGAGC AACGGAGACT AATCCTACAG 
GAAGAGGAGA GTAAACTCGC ACAACAGAGA GCCAGATTGG CCGCCATGAA GCGCGAGCTG 
AAGGTGAAGG AACTCAGTCT GATTGATTCC TCCAGAAGAC GTTTTCTCAA ACACCAGCAG 
GATCAGCGCA GAGTACACCT CAAGAGACTT GATGATGAGA TTCAGAGAAA GATGGCGTTG 
CGCGATCAGG AAACAGCGGC CGCCGTTCAA GAAGTAGAAG TCAGACAGAT GGAACTGGAT 
GCCCAGAGAC AACTGTTTGA ACAGCAACTG GCCAGAGAGC AAGAGCGAGT GAGTCAAGAG 
GTGCGAGCAG AGGTGGATAC GAGGAGCCGG AGGGCTGAGG CTGACCATTA TACATTTCAA 
AACTTCATTC TCGCAGATGC AGATATCAGC CAGAAAAACC AGAAGATGCT GGTGGAGGAA 
TCTCTGGCGG AGGCGGGGCA GATGGAGGTG GATGCTGATT GGCAGGCTGA GGTGATCAAG 
AGACTGGAAG AGGCAAAGGA GAGGCAGGAG AAGCAAAGAG AGAGACTTGT GAACCTCGGC 
AAACAAACAC GCGATAAAGA GGATAAAGTA GTCCAATTAA TGCAGGAAGT GGAGGGAAAG 
CAGTGGGATG ATATAGTAGC CAAACAAGCC GAACGTGAGG ACAAAAGATG GGCGGCTACT 
TCTGTTACCA CTCAGCCGAG GTCATTTATC TCACAGGAAA CAGAAGAGAT CGAGCAACAC 
AGCAAACCAC ACAAGACCCT TGATGATTCA CTGAATCCTC AGAAGGAAAA GAGACGTAGA 
TCTCCTTGCT CGTCCAGAGG GCTTCTGTAC AAACCCACTG ACATTTCTTC AAACAATCAG 
TCAGACTTTT CTTTAGATCG CAACAGAGGG GAGCTGGACT CAAAGGAAAG GGAGTTGATG 
CAGGAAATCA GAGAACTGAG GATGAAATTA GCAGCCAGAG CCAGAGCAAC TGATTTTATC 
TCAGCTTCAG CCAGCTCCAT ACATTCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_695006.1
CDS	308..3520
Translation	MQVTDIGSREDGKIWHRKSVSKNGVLATVIRNSSARQTKTVRFLQVAFDTTGESFLAGDHHGNIYVFDICRNRFKLVQKTGQACTALAFNLRRTTEFLVALADYSIKCFDKDTRQLVSWMRGHEGAVSSLSIHSSGSFAISTSSDTAQLWSLDTFQRKRKLNVRQSVGIQKVFFLPHSNTILSCFSDNSIFAWETDTLFCKYQLPVPQNGPRIHYRTFAVTQNGKTLAAAGRSNLVHLWCLDSQQLIRVIQMPSNVKTVRYLDFLPDSFDDGSNQTLGVLSQDGIMRFVNIQSCKQLFLIGSHEDAISSATVCPKGHHIVAVMDSGAIKIYDVRSLTTEISKPPQPLLKRVTKVNISDGSCLKMKVNTGAGQRAVKTSGRRIQSKLLPPSVSTLDDKENDLPNGLSKKRLQALLREFGEYPAKYRMFIWRSLLRLPENHAAFSSLTDKGLHSAFLCLQEQYPIKSHKLQRGLQRVLSALAHWAAVFGETDYVPLLAFPFVKLFQNNPLICFEAVATVIVNWCQHWFEYFPNPPLNVLSLAENVLAHHDKELLQHFVACGVTSQLYAWPLLETLFSEVLMREEWLKLFDNVFSNHPSFLLMAVVAYVTCCRAPLLLCTDKRDFEYFFHHRNSLDIGAMLKETYRLMDRTPADIHPSSALNDFEPLTQGQYPVFNKYPTFIVEYQSQERERIRQQEVEYLRERQEVQKLHAEAVRQQAEFEAWYKQQELLLQGEEQRRLILQEEESKLAQQRARLAAMKRELKVKELSLIDSSRRRFLKHQQDQRRVHLKRLDDEIQRKMALRDQETAAAVQEVEVRQMELDAQRQLFEQQLAREQERVSQEVRAEVDTRSRRAEADHYTFQNFILADADISQKNQKMLVEESLAEAGQMEVDADWQAEVIKRLEEAKERQEKQRERLVNLGKQTRDKEDKVVQLMQEVEGKQWDDIVAKQAEREDKRWAATSVTTQPRSFISQETEEIEQHSKPHKTLDDSLNPQKEKRRRSPCSSRGLLYKPTDISSNNQSDFSLDRNRGELDSKERELMQEIRELRMKLAARARATDFISASASSIHSH

Target ORF information:

RefSeq Version	XM_689914.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TBC1 domain family, member 31 (tbc1d31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_689914.6

ORF Insert Sequence:

ATGCAAGTTA CAGATATCGG GTCAAGAGAA GACGGGAAAA TATGGCACCG AAAATCAGTA 
TCAAAAAACG GCGTTCTGGC CACTGTGATC CGCAACTCGT CTGCTCGTCA AACCAAGACG 
GTTCGATTCC TGCAAGTGGC TTTCGACACC ACCGGAGAGT CGTTTCTTGC TGGTGATCAT 
CATGGGAATA TATATGTATT TGACATCTGC AGAAACAGAT TCAAGCTTGT CCAGAAAACA 
GGACAGGCAT GCACTGCTTT AGCCTTCAAT TTAAGAAGAA CCACTGAGTT CCTGGTTGCT 
CTGGCTGATT ACTCCATCAA GTGCTTTGAC AAAGACACCA GGCAGCTCGT GAGCTGGATG 
CGGGGTCACG AAGGTGCCGT TTCCTCCTTG TCCATCCATA GCTCAGGCAG CTTCGCCATC 
AGCACGTCTT CTGACACCGC GCAGCTCTGG AGCCTCGACA CCTTCCAGAG AAAGAGGAAG 
CTCAATGTTC GTCAGTCGGT TGGCATACAA AAGGTGTTTT TTCTCCCACA CAGCAACACC 
ATTCTTAGCT GCTTTAGCGA CAATTCCATT TTTGCTTGGG AGACCGACAC TCTCTTCTGC 
AAATATCAAC TGCCTGTTCC ACAGAATGGG CCCAGAATTC ACTACAGGAC TTTTGCTGTT 
ACACAAAATG GAAAAACACT TGCTGCTGCT GGACGATCCA ACCTTGTCCA CTTGTGGTGT 
CTGGACAGTC AACAGCTCAT CAGAGTAATT CAGATGCCTT CAAATGTCAA GACAGTAAGA 
TATCTGGATT TCCTGCCAGA TAGTTTTGAT GACGGATCCA ACCAGACTCT AGGTGTTTTA 
AGCCAAGACG GCATCATGCG CTTTGTCAAC ATCCAGTCAT GCAAGCAGCT CTTTTTAATC 
GGATCCCACG AGGATGCAAT CAGCTCTGCA ACTGTTTGTC CTAAAGGTCA CCATATTGTC 
GCAGTGATGG ACAGTGGAGC TATTAAAATC TATGATGTGC GAAGTCTCAC CACAGAAATC 
AGTAAGCCTC CTCAGCCTTT GCTGAAACGG GTCACTAAAG TTAACATCAG TGATGGATCA 
TGTCTGAAAA TGAAGGTTAA CACTGGGGCA GGTCAGCGCG CTGTGAAAAC ATCAGGACGC 
AGAATCCAAT CCAAACTTTT ACCACCTTCA GTTTCTACTC TGGATGACAA AGAGAATGAT 
TTGCCTAATG GCCTAAGCAA GAAAAGACTA CAAGCGTTGC TAAGAGAGTT TGGCGAGTAT 
CCAGCAAAAT ACAGAATGTT TATTTGGCGT TCCCTGTTGC GTTTACCAGA GAACCATGCA 
GCTTTCAGCA GCTTGACTGA TAAAGGCCTG CATTCAGCCT TCCTGTGTCT TCAGGAGCAG 
TACCCCATCA AAAGCCACAA ACTACAAAGA GGCCTGCAGA GAGTGTTGTC TGCTCTTGCT 
CACTGGGCTG CAGTCTTCGG TGAGACTGAT TATGTCCCCC TGCTGGCCTT CCCATTCGTC 
AAGCTGTTCC AGAACAATCC TCTGATCTGC TTTGAAGCAG TGGCCACTGT CATTGTGAAC 
TGGTGCCAGC ACTGGTTTGA GTATTTTCCC AACCCTCCGC TTAATGTGCT GAGTCTGGCT 
GAGAATGTGC TGGCCCATCA TGATAAAGAG CTGCTTCAGC ATTTTGTTGC CTGTGGCGTT 
ACCTCACAGC TGTATGCGTG GCCTCTTCTA GAAACTCTGT TCTCTGAGGT TCTGATGAGA 
GAAGAGTGGC TGAAGCTATT TGACAATGTT TTCTCCAATC ATCCCTCATT CCTGCTAATG 
GCTGTGGTGG CGTATGTCAC CTGCTGCCGA GCACCGCTAC TGCTCTGCAC TGACAAGAGA 
GATTTTGAGT ACTTTTTCCA TCATCGCAAT AGTTTGGACA TTGGTGCTAT GCTGAAGGAA 
ACCTATCGGT TGATGGACAG AACCCCTGCC GACATCCATC CTAGCTCTGC GCTGAATGAT 
TTTGAACCTC TGACTCAGGG CCAGTATCCT GTCTTCAACA AATACCCCAC ATTCATAGTG 
GAGTACCAGT CTCAAGAACG CGAGAGGATC CGCCAACAAG AAGTGGAATA TCTGAGAGAG 
AGACAGGAGG TTCAGAAGCT GCATGCTGAG GCGGTCAGAC AGCAGGCTGA GTTTGAGGCC 
TGGTATAAAC AGCAGGAGCT GCTTCTGCAA GGAGAAGAGC AACGGAGACT AATCCTACAG 
GAAGAGGAGA GTAAACTCGC ACAACAGAGA GCCAGATTGG CCGCCATGAA GCGCGAGCTG 
AAGGTGAAGG AACTCAGTCT GATTGATTCC TCCAGAAGAC GTTTTCTCAA ACACCAGCAG 
GATCAGCGCA GAGTACACCT CAAGAGACTT GATGATGAGA TTCAGAGAAA GATGGCGTTG 
CGCGATCAGG AAACAGCGGC CGCCGTTCAA GAAGTAGAAG TCAGACAGAT GGAACTGGAT 
GCCCAGAGAC AACTGTTTGA ACAGCAACTG GCCAGAGAGC AAGAGCGAGT GAGTCAAGAG 
GTGCGAGCAG AGGTGGATAC GAGGAGCCGG AGGGCTGAGG CTGACCATTA TACATTTCAA 
AACTTCATTC TCGCAGATGC AGATATCAGC CAGAAAAACC AGAAGATGCT GGTGGAGGAA 
TCTCTGGCGG AGGCGGGGCA GATGGAGGTG GATGCTGATT GGCAGGCTGA GGTGATCAAG 
AGACTGGAAG AGGCAAAGGA GAGGCAGGAG AAGCAAAGAG AGAGACTTGT GAACCTCGGC 
AAACAAACAC GCGATAAAGA GGATAAAGTA GTCCAATTAA TGCAGGAAGT GGAGGGAAAG 
CAGTGGGATG ATATAGTAGC CAAACAAGCC GAACGTGAGG ACAAAAGATG GGCGGCTACT 
TCTGTTACCA CTCAGCCGAG GTCATTTATC TCACAGGAAA CAGAAGAGAT CGAGCAACAC 
AGCAAACCAC ACAAGACCCT TGATGATTCA CTGAATCCTC AGAAGGAAAA GAGACGTAGA 
TCTCCTTGCT CGTCCAGAGG GCTTCTGTAC AAACCCACTG ACATTTCTTC AAACAATCAG 
TCAGACTTTT CTTTAGATCG CAACAGAGGG GAGCTGGACT CAAAGGAAAG GGAGTTGATG 
CAGGAAATCA GAGAACTGAG GATGAAATTA GCAGCCAGAG CCAGAGCAAC TGATTTTATC 
TCAGCTTCAG CCAGCTCCAT ACATTCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBC1D31 ( XM_689914.5 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_695006.1 Danio rerio TBC1 domain family, member 31 (tbc1d31), mRNA.

Price & Availability↑

CloneID	ODa21494
Clone ID Related Accession (Same CDS sequence)	XM_689914.6 , XM_689914.5
Accession Version	XM_689914.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	TBC1 domain family member 31
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_689914.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTTA CAGATATCGG GTCAAGAGAA GACGGGAAAA TATGGCACCG AAAATCAGTA 
TCAAAAAACG GCGTTCTGGC CACTGTGATC CGCAACTCGT CTGCTCGTCA AACCAAGACG 
GTTCGATTCC TGCAAGTGGC TTTCGACACC ACCGGAGAGT CGTTTCTTGC TGGTGATCAT 
CATGGGAATA TATATGTATT TGACATCTGC AGAAACAGAT TCAAGCTTGT CCAGAAAACA 
GGACAGGCAT GCACTGCTTT AGCCTTCAAT TTAAGAAGAA CCACTGAGTT CCTGGTTGCT 
CTGGCTGATT ACTCCATCAA GTGCTTTGAC AAAGACACCA GGCAGCTCGT GAGCTGGATG 
CGGGGTCACG AAGGTGCCGT TTCCTCCTTG TCCATCCATA GCTCAGGCAG CTTCGCCATC 
AGCACGTCTT CTGACACCGC GCAGCTCTGG AGCCTCGACA CCTTCCAGAG AAAGAGGAAG 
CTCAATGTTC GTCAGTCGGT TGGCATACAA AAGGTGTTTT TTCTCCCACA CAGCAACACC 
ATTCTTAGCT GCTTTAGCGA CAATTCCATT TTTGCTTGGG AGACCGACAC TCTCTTCTGC 
AAATATCAAC TGCCTGTTCC ACAGAATGGG CCCAGAATTC ACTACAGGAC TTTTGCTGTT 
ACACAAAATG GAAAAACACT TGCTGCTGCT GGACGATCCA ACCTTGTCCA CTTGTGGTGT 
CTGGACAGTC AACAGCTCAT CAGAGTAATT CAGATGCCTT CAAATGTCAA GACAGTAAGA 
TATCTGGATT TCCTGCCAGA TAGTTTTGAT GACGGATCCA ACCAGACTCT AGGTGTTTTA 
AGCCAAGACG GCATCATGCG CTTTGTCAAC ATCCAGTCAT GCAAGCAGCT CTTTTTAATC 
GGATCCCACG AGGATGCAAT CAGCTCTGCA ACTGTTTGTC CTAAAGGTCA CCATATTGTC 
GCAGTGATGG ACAGTGGAGC TATTAAAATC TATGATGTGC GAAGTCTCAC CACAGAAATC 
AGTAAGCCTC CTCAGCCTTT GCTGAAACGG GTCACTAAAG TTAACATCAG TGATGGATCA 
TGTCTGAAAA TGAAGGTTAA CACTGGGGCA GGTCAGCGCG CTGTGAAAAC ATCAGGACGC 
AGAATCCAAT CCAAACTTTT ACCACCTTCA GTTTCTACTC TGGATGACAA AGAGAATGAT 
TTGCCTAATG GCCTAAGCAA GAAAAGACTA CAAGCGTTGC TAAGAGAGTT TGGCGAGTAT 
CCAGCAAAAT ACAGAATGTT TATTTGGCGT TCCCTGTTGC GTTTACCAGA GAACCATGCA 
GCTTTCAGCA GCTTGACTGA TAAAGGCCTG CATTCAGCCT TCCTGTGTCT TCAGGAGCAG 
TACCCCATCA AAAGCCACAA ACTACAAAGA GGCCTGCAGA GAGTGTTGTC TGCTCTTGCT 
CACTGGGCTG CAGTCTTCGG TGAGACTGAT TATGTCCCCC TGCTGGCCTT CCCATTCGTC 
AAGCTGTTCC AGAACAATCC TCTGATCTGC TTTGAAGCAG TGGCCACTGT CATTGTGAAC 
TGGTGCCAGC ACTGGTTTGA GTATTTTCCC AACCCTCCGC TTAATGTGCT GAGTCTGGCT 
GAGAATGTGC TGGCCCATCA TGATAAAGAG CTGCTTCAGC ATTTTGTTGC CTGTGGCGTT 
ACCTCACAGC TGTATGCGTG GCCTCTTCTA GAAACTCTGT TCTCTGAGGT TCTGATGAGA 
GAAGAGTGGC TGAAGCTATT TGACAATGTT TTCTCCAATC ATCCCTCATT CCTGCTAATG 
GCTGTGGTGG CGTATGTCAC CTGCTGCCGA GCACCGCTAC TGCTCTGCAC TGACAAGAGA 
GATTTTGAGT ACTTTTTCCA TCATCGCAAT AGTTTGGACA TTGGTGCTAT GCTGAAGGAA 
ACCTATCGGT TGATGGACAG AACCCCTGCC GACATCCATC CTAGCTCTGC GCTGAATGAT 
TTTGAACCTC TGACTCAGGG CCAGTATCCT GTCTTCAACA AATACCCCAC ATTCATAGTG 
GAGTACCAGT CTCAAGAACG CGAGAGGATC CGCCAACAAG AAGTGGAATA TCTGAGAGAG 
AGACAGGAGG TTCAGAAGCT GCATGCTGAG GCGGTCAGAC AGCAGGCTGA GTTTGAGGCC 
TGGTATAAAC AGCAGGAGCT GCTTCTGCAA GGAGAAGAGC AACGGAGACT AATCCTACAG 
GAAGAGGAGA GTAAACTCGC ACAACAGAGA GCCAGATTGG CCGCCATGAA GCGCGAGCTG 
AAGGTGAAGG AACTCAGTCT GATTGATTCC TCCAGAAGAC GTTTTCTCAA ACACCAGCAG 
GATCAGCGCA GAGTACACCT CAAGAGACTT GATGATGAGA TTCAGAGAAA GATGGCGTTG 
CGCGATCAGG AAACAGCGGC CGCCGTTCAA GAAGTAGAAG TCAGACAGAT GGAACTGGAT 
GCCCAGAGAC AACTGTTTGA ACAGCAACTG GCCAGAGAGC AAGAGCGAGT GAGTCAAGAG 
GTGCGAGCAG AGGTGGATAC GAGGAGCCGG AGGGCTGAGG CTGACCATTA TACATTTCAA 
AACTTCATTC TCGCAGATGC AGATATCAGC CAGAAAAACC AGAAGATGCT GGTGGAGGAA 
TCTCTGGCGG AGGCGGGGCA GATGGAGGTG GATGCTGATT GGCAGGCTGA GGTGATCAAG 
AGACTGGAAG AGGCAAAGGA GAGGCAGGAG AAGCAAAGAG AGAGACTTGT GAACCTCGGC 
AAACAAACAC GCGATAAAGA GGATAAAGTA GTCCAATTAA TGCAGGAAGT GGAGGGAAAG 
CAGTGGGATG ATATAGTAGC CAAACAAGCC GAACGTGAGG ACAAAAGATG GGCGGCTACT 
TCTGTTACCA CTCAGCCGAG GTCATTTATC TCACAGGAAA CAGAAGAGAT CGAGCAACAC 
AGCAAACCAC ACAAGACCCT TGATGATTCA CTGAATCCTC AGAAGGAAAA GAGACGTAGA 
TCTCCTTGCT CGTCCAGAGG GCTTCTGTAC AAACCCACTG ACATTTCTTC AAACAATCAG 
TCAGACTTTT CTTTAGATCG CAACAGAGGG GAGCTGGACT CAAAGGAAAG GGAGTTGATG 
CAGGAAATCA GAGAACTGAG GATGAAATTA GCAGCCAGAG CCAGAGCAAC TGATTTTATC 
TCAGCTTCAG CCAGCTCCAT ACATTCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_695006.1
CDS	322..3534
Translation	MQVTDIGSREDGKIWHRKSVSKNGVLATVIRNSSARQTKTVRFLQVAFDTTGESFLAGDHHGNIYVFDICRNRFKLVQKTGQACTALAFNLRRTTEFLVALADYSIKCFDKDTRQLVSWMRGHEGAVSSLSIHSSGSFAISTSSDTAQLWSLDTFQRKRKLNVRQSVGIQKVFFLPHSNTILSCFSDNSIFAWETDTLFCKYQLPVPQNGPRIHYRTFAVTQNGKTLAAAGRSNLVHLWCLDSQQLIRVIQMPSNVKTVRYLDFLPDSFDDGSNQTLGVLSQDGIMRFVNIQSCKQLFLIGSHEDAISSATVCPKGHHIVAVMDSGAIKIYDVRSLTTEISKPPQPLLKRVTKVNISDGSCLKMKVNTGAGQRAVKTSGRRIQSKLLPPSVSTLDDKENDLPNGLSKKRLQALLREFGEYPAKYRMFIWRSLLRLPENHAAFSSLTDKGLHSAFLCLQEQYPIKSHKLQRGLQRVLSALAHWAAVFGETDYVPLLAFPFVKLFQNNPLICFEAVATVIVNWCQHWFEYFPNPPLNVLSLAENVLAHHDKELLQHFVACGVTSQLYAWPLLETLFSEVLMREEWLKLFDNVFSNHPSFLLMAVVAYVTCCRAPLLLCTDKRDFEYFFHHRNSLDIGAMLKETYRLMDRTPADIHPSSALNDFEPLTQGQYPVFNKYPTFIVEYQSQERERIRQQEVEYLRERQEVQKLHAEAVRQQAEFEAWYKQQELLLQGEEQRRLILQEEESKLAQQRARLAAMKRELKVKELSLIDSSRRRFLKHQQDQRRVHLKRLDDEIQRKMALRDQETAAAVQEVEVRQMELDAQRQLFEQQLAREQERVSQEVRAEVDTRSRRAEADHYTFQNFILADADISQKNQKMLVEESLAEAGQMEVDADWQAEVIKRLEEAKERQEKQRERLVNLGKQTRDKEDKVVQLMQEVEGKQWDDIVAKQAEREDKRWAATSVTTQPRSFISQETEEIEQHSKPHKTLDDSLNPQKEKRRRSPCSSRGLLYKPTDISSNNQSDFSLDRNRGELDSKERELMQEIRELRMKLAARARATDFISASASSIHSH

Target ORF information:

RefSeq Version	XM_689914.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio TBC1 domain family, member 31 (tbc1d31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_689914.5

ORF Insert Sequence:

ATGCAAGTTA CAGATATCGG GTCAAGAGAA GACGGGAAAA TATGGCACCG AAAATCAGTA 
TCAAAAAACG GCGTTCTGGC CACTGTGATC CGCAACTCGT CTGCTCGTCA AACCAAGACG 
GTTCGATTCC TGCAAGTGGC TTTCGACACC ACCGGAGAGT CGTTTCTTGC TGGTGATCAT 
CATGGGAATA TATATGTATT TGACATCTGC AGAAACAGAT TCAAGCTTGT CCAGAAAACA 
GGACAGGCAT GCACTGCTTT AGCCTTCAAT TTAAGAAGAA CCACTGAGTT CCTGGTTGCT 
CTGGCTGATT ACTCCATCAA GTGCTTTGAC AAAGACACCA GGCAGCTCGT GAGCTGGATG 
CGGGGTCACG AAGGTGCCGT TTCCTCCTTG TCCATCCATA GCTCAGGCAG CTTCGCCATC 
AGCACGTCTT CTGACACCGC GCAGCTCTGG AGCCTCGACA CCTTCCAGAG AAAGAGGAAG 
CTCAATGTTC GTCAGTCGGT TGGCATACAA AAGGTGTTTT TTCTCCCACA CAGCAACACC 
ATTCTTAGCT GCTTTAGCGA CAATTCCATT TTTGCTTGGG AGACCGACAC TCTCTTCTGC 
AAATATCAAC TGCCTGTTCC ACAGAATGGG CCCAGAATTC ACTACAGGAC TTTTGCTGTT 
ACACAAAATG GAAAAACACT TGCTGCTGCT GGACGATCCA ACCTTGTCCA CTTGTGGTGT 
CTGGACAGTC AACAGCTCAT CAGAGTAATT CAGATGCCTT CAAATGTCAA GACAGTAAGA 
TATCTGGATT TCCTGCCAGA TAGTTTTGAT GACGGATCCA ACCAGACTCT AGGTGTTTTA 
AGCCAAGACG GCATCATGCG CTTTGTCAAC ATCCAGTCAT GCAAGCAGCT CTTTTTAATC 
GGATCCCACG AGGATGCAAT CAGCTCTGCA ACTGTTTGTC CTAAAGGTCA CCATATTGTC 
GCAGTGATGG ACAGTGGAGC TATTAAAATC TATGATGTGC GAAGTCTCAC CACAGAAATC 
AGTAAGCCTC CTCAGCCTTT GCTGAAACGG GTCACTAAAG TTAACATCAG TGATGGATCA 
TGTCTGAAAA TGAAGGTTAA CACTGGGGCA GGTCAGCGCG CTGTGAAAAC ATCAGGACGC 
AGAATCCAAT CCAAACTTTT ACCACCTTCA GTTTCTACTC TGGATGACAA AGAGAATGAT 
TTGCCTAATG GCCTAAGCAA GAAAAGACTA CAAGCGTTGC TAAGAGAGTT TGGCGAGTAT 
CCAGCAAAAT ACAGAATGTT TATTTGGCGT TCCCTGTTGC GTTTACCAGA GAACCATGCA 
GCTTTCAGCA GCTTGACTGA TAAAGGCCTG CATTCAGCCT TCCTGTGTCT TCAGGAGCAG 
TACCCCATCA AAAGCCACAA ACTACAAAGA GGCCTGCAGA GAGTGTTGTC TGCTCTTGCT 
CACTGGGCTG CAGTCTTCGG TGAGACTGAT TATGTCCCCC TGCTGGCCTT CCCATTCGTC 
AAGCTGTTCC AGAACAATCC TCTGATCTGC TTTGAAGCAG TGGCCACTGT CATTGTGAAC 
TGGTGCCAGC ACTGGTTTGA GTATTTTCCC AACCCTCCGC TTAATGTGCT GAGTCTGGCT 
GAGAATGTGC TGGCCCATCA TGATAAAGAG CTGCTTCAGC ATTTTGTTGC CTGTGGCGTT 
ACCTCACAGC TGTATGCGTG GCCTCTTCTA GAAACTCTGT TCTCTGAGGT TCTGATGAGA 
GAAGAGTGGC TGAAGCTATT TGACAATGTT TTCTCCAATC ATCCCTCATT CCTGCTAATG 
GCTGTGGTGG CGTATGTCAC CTGCTGCCGA GCACCGCTAC TGCTCTGCAC TGACAAGAGA 
GATTTTGAGT ACTTTTTCCA TCATCGCAAT AGTTTGGACA TTGGTGCTAT GCTGAAGGAA 
ACCTATCGGT TGATGGACAG AACCCCTGCC GACATCCATC CTAGCTCTGC GCTGAATGAT 
TTTGAACCTC TGACTCAGGG CCAGTATCCT GTCTTCAACA AATACCCCAC ATTCATAGTG 
GAGTACCAGT CTCAAGAACG CGAGAGGATC CGCCAACAAG AAGTGGAATA TCTGAGAGAG 
AGACAGGAGG TTCAGAAGCT GCATGCTGAG GCGGTCAGAC AGCAGGCTGA GTTTGAGGCC 
TGGTATAAAC AGCAGGAGCT GCTTCTGCAA GGAGAAGAGC AACGGAGACT AATCCTACAG 
GAAGAGGAGA GTAAACTCGC ACAACAGAGA GCCAGATTGG CCGCCATGAA GCGCGAGCTG 
AAGGTGAAGG AACTCAGTCT GATTGATTCC TCCAGAAGAC GTTTTCTCAA ACACCAGCAG 
GATCAGCGCA GAGTACACCT CAAGAGACTT GATGATGAGA TTCAGAGAAA GATGGCGTTG 
CGCGATCAGG AAACAGCGGC CGCCGTTCAA GAAGTAGAAG TCAGACAGAT GGAACTGGAT 
GCCCAGAGAC AACTGTTTGA ACAGCAACTG GCCAGAGAGC AAGAGCGAGT GAGTCAAGAG 
GTGCGAGCAG AGGTGGATAC GAGGAGCCGG AGGGCTGAGG CTGACCATTA TACATTTCAA 
AACTTCATTC TCGCAGATGC AGATATCAGC CAGAAAAACC AGAAGATGCT GGTGGAGGAA 
TCTCTGGCGG AGGCGGGGCA GATGGAGGTG GATGCTGATT GGCAGGCTGA GGTGATCAAG 
AGACTGGAAG AGGCAAAGGA GAGGCAGGAG AAGCAAAGAG AGAGACTTGT GAACCTCGGC 
AAACAAACAC GCGATAAAGA GGATAAAGTA GTCCAATTAA TGCAGGAAGT GGAGGGAAAG 
CAGTGGGATG ATATAGTAGC CAAACAAGCC GAACGTGAGG ACAAAAGATG GGCGGCTACT 
TCTGTTACCA CTCAGCCGAG GTCATTTATC TCACAGGAAA CAGAAGAGAT CGAGCAACAC 
AGCAAACCAC ACAAGACCCT TGATGATTCA CTGAATCCTC AGAAGGAAAA GAGACGTAGA 
TCTCCTTGCT CGTCCAGAGG GCTTCTGTAC AAACCCACTG ACATTTCTTC AAACAATCAG 
TCAGACTTTT CTTTAGATCG CAACAGAGGG GAGCTGGACT CAAAGGAAAG GGAGTTGATG 
CAGGAAATCA GAGAACTGAG GATGAAATTA GCAGCCAGAG CCAGAGCAAC TGATTTTATC 
TCAGCTTCAG CCAGCTCCAT ACATTCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TBC1D31 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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