gnb5a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gnb5a
Entrez Gene ID	562813
Full Name	guanine nucleotide binding protein (G protein), beta 5a
Synonyms	zgc:158678
General protein information	Preferred Names guanine nucleotide binding protein (G protein), beta 5a Names guanine nucleotide-binding protein subunit beta-5a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001080181.1	NP_001073650.1	guanine nucleotide-binding protein subunit beta-5a

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	GNB5	XP_001085617.2
Danio rerio (zebrafish)	gnb5a	NP_001073650.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

GNB5 Mutations Cause an Autosomal-Recessive Multisystem Syndrome with Sinus Bradycardia and Cognitive Disability.
Lodder EM, De Nittis P, Koopman CD, Wiszniewski W, Moura de Souza CF, Lahrouchi N, Guex N, Napolioni V, Tessadori F, Beekman L, Nannenberg EA, Boualla L, Blom NA, de Graaff W, Kamermans M, Cocciadiferro D, Malerba N, Mandriani B, Akdemir ZHC, Fish RJ, Eldomery MK, Ratbi I, Wilde AAM, de Boer T, Simonds WF, Neerman-Arbez M, Sutton VR, Kok F, Lupski JR, Reymond A, Bezzina CR, Bakkers J, Merla G
American journal of human genetics99(3)704-710(2016 09)

Gβγ signaling controls the polarization of zebrafish primordial germ cells by regulating Rac activity.
Xu H, Kardash E, Chen S, Raz E, Lin F
Development (Cambridge, England)139(1)57-62(2012 Jan)

Prenylation-deficient G protein gamma subunits disrupt GPCR signaling in the zebrafish.
Mulligan T, Blaser H, Raz E, Farber SA
Cellular signalling22(2)221-33(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gnb5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gnb5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa32522	NM_001080181.1 Latest version!	Danio rerio guanine nucleotide binding protein (G protein), beta 5a (gnb5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gnb5a ( NM_001080181.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001073650.1 Danio rerio guanine nucleotide binding protein (G protein), beta 5a (gnb5a), mRNA.

Price & Availability↑

CloneID	ODa32522
Clone ID Related Accession (Same CDS sequence)	NM_001080181.1
Accession Version	NM_001080181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548432000000
Organism	Danio rerio(zebrafish)
Product	guanine nucleotide-binding protein subunit beta-5a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC129375.1. On Mar 11, 2007 this sequence version replaced XM_686187.2. ##Evidence-Data-START## Transcript exon combination :: BC129375.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGC AGGAGGAGCC CGCGCAGCCC GGAGACTCTC TGGCGACGCT GAAGAGCGAG 
TCCGACACCC TGAAGAGCAA ACTGGAGGAG GAGAGAGCCA AACTACACGA TGTCGAGCTG 
CATCAGGTGG CGGAGAAGAT TGAAGCTCTG GGTCAGTTCG TCATGAAGAC CAGACGGACT 
CTGAAAGGCC ATGGAAATAA AGTCTTGTGT ATGGACTGGT GTAAAGACAA GCGGAGGATC 
GTCAGCTCGT CTCAGGATGG AAAGGTGATT GTGTGGGATG CGTTTACCAC AAATAAGGAG 
CATGCTGTGA CTATGCCCTG TACGTGGGTC ATGGCCTGTG CTTACGCCCC ATCTGGATGT 
GCAGTGGCTT GTGGCGGGCT GGACAATAAG TGCTCGGTGT ATCCTTTATC CCTGGACAAG 
AACGAAAACC TCGCAGCTAA GAAGAAATCA GTGGCCATGC ACACCAACTA TCTGTCAGCC 
TGCTGCTTCA CCAACTCAGA CATGCAGATC CTGACGTCCA GTGGTGATGG CACGTGTGCT 
CTGTGGGATG TGGAAAGTGG GCAGATGCTG CAGAGTTTTC ATGGACACGC GGCTGATGTG 
CTGTGTCTGG ATCTCGCTCC GTCTGAGACC GGAAACACAT TCGTTTCAGG GGGCTGTGAT 
AAGAAGGCCT GTGTGTGGGA CATGCGCACA GGACAGTGCG TCCAGTCTTT CGAGAGTCAC 
GATTCAGACA TCAACAGCGT GCGGTACTAT CCAAGCGGAG ATGCTTTTGC TTCAGGCTCA 
GACGACGCTA CTTGTCGTCT GTATGACCTC AGGGCGGACA GAGAGGTGGC CATTTATTCC 
AAAGAGAGCA TCATATTCGG GGCTTCTAGT GTGGATTTCT CTCTCAGCGG GAGACTGTTG 
TTTGGCGGCT ACAATGACTA CACGATCAAC GTGTGGGATG TTTTAAAGGG AGCCCGCGTG 
TCCATCTTGT TTGGGCATGA GAACAGAGTC AGCACTCTTC GCGTTTCTCC AGATGGGACT 
GCGTTCTGCT CAGGATCCTG GGATCACACG TTACGGATCT GGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073650.1
CDS	101..1168
Translation	MAAQEEPAQPGDSLATLKSESDTLKSKLEEERAKLHDVELHQVAEKIEALGQFVMKTRRTLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDAFTTNKEHAVTMPCTWVMACAYAPSGCAVACGGLDNKCSVYPLSLDKNENLAAKKKSVAMHTNYLSACCFTNSDMQILTSSGDGTCALWDVESGQMLQSFHGHAADVLCLDLAPSETGNTFVSGGCDKKACVWDMRTGQCVQSFESHDSDINSVRYYPSGDAFASGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFGGYNDYTINVWDVLKGARVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRIWA

Target ORF information:

RefSeq Version	NM_001080181.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio guanine nucleotide binding protein (G protein), beta 5a (gnb5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080181.1

ORF Insert Sequence:

ATGGCGGCGC AGGAGGAGCC CGCGCAGCCC GGAGACTCTC TGGCGACGCT GAAGAGCGAG 
TCCGACACCC TGAAGAGCAA ACTGGAGGAG GAGAGAGCCA AACTACACGA TGTCGAGCTG 
CATCAGGTGG CGGAGAAGAT TGAAGCTCTG GGTCAGTTCG TCATGAAGAC CAGACGGACT 
CTGAAAGGCC ATGGAAATAA AGTCTTGTGT ATGGACTGGT GTAAAGACAA GCGGAGGATC 
GTCAGCTCGT CTCAGGATGG AAAGGTGATT GTGTGGGATG CGTTTACCAC AAATAAGGAG 
CATGCTGTGA CTATGCCCTG TACGTGGGTC ATGGCCTGTG CTTACGCCCC ATCTGGATGT 
GCAGTGGCTT GTGGCGGGCT GGACAATAAG TGCTCGGTGT ATCCTTTATC CCTGGACAAG 
AACGAAAACC TCGCAGCTAA GAAGAAATCA GTGGCCATGC ACACCAACTA TCTGTCAGCC 
TGCTGCTTCA CCAACTCAGA CATGCAGATC CTGACGTCCA GTGGTGATGG CACGTGTGCT 
CTGTGGGATG TGGAAAGTGG GCAGATGCTG CAGAGTTTTC ATGGACACGC GGCTGATGTG 
CTGTGTCTGG ATCTCGCTCC GTCTGAGACC GGAAACACAT TCGTTTCAGG GGGCTGTGAT 
AAGAAGGCCT GTGTGTGGGA CATGCGCACA GGACAGTGCG TCCAGTCTTT CGAGAGTCAC 
GATTCAGACA TCAACAGCGT GCGGTACTAT CCAAGCGGAG ATGCTTTTGC TTCAGGCTCA 
GACGACGCTA CTTGTCGTCT GTATGACCTC AGGGCGGACA GAGAGGTGGC CATTTATTCC 
AAAGAGAGCA TCATATTCGG GGCTTCTAGT GTGGATTTCT CTCTCAGCGG GAGACTGTTG 
TTTGGCGGCT ACAATGACTA CACGATCAAC GTGTGGGATG TTTTAAAGGG AGCCCGCGTG 
TCCATCTTGT TTGGGCATGA GAACAGAGTC AGCACTCTTC GCGTTTCTCC AGATGGGACT 
GCGTTCTGCT CAGGATCCTG GGATCACACG TTACGGATCT GGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

GNB5 Mutations Cause an Autosomal-Recessive Multisystem Syndrome with Sinus Bradycardia and Cognitive Disability.
American journal of human genetics99(3)704-710(2016 09)
Lodder EM,De Nittis P,Koopman CD,Wiszniewski W,Moura de Souza CF,Lahrouchi N,Guex N,Napolioni V,Tessadori F,Beekman L,Nannenberg EA,Boualla L,Blom NA,de Graaff W,Kamermans M,Cocciadiferro D,Malerba N,Mandriani B,Akdemir ZHC,Fish RJ,Eldomery MK,Ratbi I,Wilde AAM,de Boer T,Simonds WF,Neerman-Arbez M,Sutton VR,Kok F,Lupski JR,Reymond A,Bezzina CR,Bakkers J,Merla G

Gβγ signaling controls the polarization of zebrafish primordial germ cells by regulating Rac activity.
Development (Cambridge, England)139(1)57-62(2012 Jan)
Xu H,Kardash E,Chen S,Raz E,Lin F

Prenylation-deficient G protein gamma subunits disrupt GPCR signaling in the zebrafish.
Cellular signalling22(2)221-33(2010 Feb)
Mulligan T,Blaser H,Raz E,Farber SA

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gnb5a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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