arhgap23b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	arhgap23b
Entrez Gene ID	562244
Full Name	Rho GTPase activating protein 23b
Synonyms	arhgap23,si:dkey-24h18.1
General protein information	Preferred Names Rho GTPase activating protein 23b Names rho GTPase-activating protein 23 Rho GTPase activating protein 23
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_685638.8	XP_690730.6	rho GTPase-activating protein 23
XM_685638.9	XP_690730.6	rho GTPase-activating protein 23

Pathways from BioSystems
Gene Ontology

The following arhgap23b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgap23b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa17719	XM_685638.9 Latest version!	Danio rerio Rho GTPase activating protein 23b (arhgap23b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa17719	XM_685638.8	Danio rerio Rho GTPase activating protein 23b (arhgap23b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arhgap23b ( XM_685638.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_690730.6 Danio rerio Rho GTPase activating protein 23b (arhgap23b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17719
Clone ID Related Accession (Same CDS sequence)	XM_685638.8 , XM_685638.9
Accession Version	XM_685638.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 23
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_685638.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAGG GTAAAGATCA CAAAAATGAA GGATTGTCGT CTAGTCAGAA GACTCGACCA 
GGAGAGACCA TGGCCTGGAA GGGTCCTCTC ACAGTGGTTC TGCGTAAAAA CTCTTTGGGC 
TTTGGTTTCA CATTGCGTCA TTTCATAGTC TATCCACCAG AGTCTGCCTT CAACACTAAC 
CTGAAGGATG AAGAAAATGG AAATGGCAAA GGAGGTCAGC AGTTTCGTTT TGAGCCAGTG 
GATACCATCT TTGTAAAGAA TGTGAAAGAG GAAGGACCCG CTCATCAGGC AGGCCTCTGC 
ACAGGTGACA GACTAGTGAG GGTGAATGGG GAAAGTGTGC TGGGGAAAAC GTTCTCTCAG 
GTCATAGCAT TGATTCAGAA TAGTGACAAT GTGCTGGAGT TGTCCATCAT GCCAAAAGAT 
GAGGATGTGC TACAGCTGGC ATATTCTCAA GATGCCTACT TGAGAGGGAA TGAGCCATAC 
ACTGGAGAAA CACAAAACCT TCCCGATCCT CCTCCAGTCT GTAACTTAAA CAGCAAATCT 
CCCTCAACAT CGCAGAACCA TGCTATTCCT GTTAACATGA ACTCTGAGAT TGTTGTAGTG 
TCTCAACCTT CCCCTCACTG CCTGATTCGT CACCGCATCT GCTCTTCATC CTCAGGTGGG 
AAAACAAACC TGCTGGACTT CCACTTTGCC AACCACAATG CAGCTATTGC CTCTGCCTCA 
CTAGTTCACC CTAGGAAAGC CAGTCTTCCC CAAGGTCACA GTGAACTGTG CCACCAAGGT 
CTGTCAGACT GGTACTATAG CCAAGTAGAC CAATCAAGCC TTGGCAATTC CCATCATTAC 
CAGAGCAGCT CTCAAGACAG GCTTTATGAA CTGGGACTGG CACCAGGAGG GTCTCGTGGT 
GGTCGGACAC ACAACTCATC TCAGGATGCC CTACTACATT ACAGTGCAGT GATGGGGATC 
CACCATTCAC CTTGCACAGA TTCTTATGGT CTTGGGAGAA CAAGTAAACT CTCTAGCCAT 
TCCTGTTCTG AAAACTTGCT AGCTGCTTAT GCTTCGTACG AGCAGAGCTT TGGTCATTCA 
CTGGAAACTC TAGAAAAGGC ATCCATTCTG ATCTCACCCC ATTATAAAAT ACCAACACAG 
CCTCATGAAA TCACTCAGCT AATCAGGGTA GCGGAGTGCA AAAGTATTGC CAATAATCAC 
ACAAATGGTT CCGCAGTTTT GCAACCATCA GGTGAGGGTT TAAGGCAAAA ACAGCAGCCA 
GCTGTTCACA TCAGACATTT ACCTGCACAA TATGTTGAGG GTCAGATGGT TGGCTGCCAC 
ACCTCCATTC CAAAAACCAT CCCACTGCAG AATCCATCCT CCATCACTCC CTCTGCTGCA 
GAGTCCCAAG AGCCCCAACG CTCACTGGTC ACTGTGTTTA CTGAGGAACC TTTACAGCTT 
AAAAAAAATC TCCCCTCAGG AATTGAGACA CACCATTGTA CTTCTGTGAA TTTTATTAAA 
CCCCTTTCAG CTAAAGTTCT TCCCTCAAGG TCAGAATATT CTGTACACAA AACTAATAGT 
GGCATTACAC CCTCATCTGT GGAGCAGGAC TCCTTGGCTG TCATCCCATT CAAAGGCAGT 
CTGAAAAAAA GCCGTCGCTC CTCCTACATG CTGGCCATTA CTACGGAGCG ATCTAAATCA 
TGTGACGAGG GTCTGAACAC TTTTAGGGAA GAAGAACGTG TTCTCTTGAA ACTGCCAAAA 
AGAGTCAAAA GCTTTTTTAC AGATGATTCT CTAGAGAGTC TTAAAGTGGC AGAAGATGCA 
CACTCTAAAC GCCACTCCAC TTCTGAACTG GGAAACATCA ATCTTACAGA CATCAGAAAA 
CAAGGCTGGC TGCATTATAA ACAGATTCTT AATGATAAAC GGAAGAAAGT GGGCAGTGGT 
ATGCGGCAGT GGAAAAGAGT ATTTTCTGTG CTGCACTCTA GTTTACTGTT CCTCTACAAG 
GATAAGCGTG AGGCGGTGCT TCATGCAACT AGGGCAGGAA GTCATGGCAT TGGGCAAGAG 
AAGGATATTC AAACAATCAG CATTCAAGGC TGCTTGATTG ACATTGCATA CAGCGAGACT 
AAGCGTAAAC ACACTTTTCG GCTGATGACT CAGGACTTCT GTGAGTATCT TCTGCAGGCA 
GAGGACAGAG ATGACATGCT GATGTGGATA AGAGTTATCA GAGAGAAAAG CAAGACCGAA 
AATGAGGAAC TGGGCTTCTC CAGACAGGTT CTCATCAGTA AGAAGCTGAA TGACTATAAG 
AAACAGAGTC CAACAAGCAA CAAGCCAGAT ACTTCTCCTA AGATGCATCA AATGATGCCA 
CCTTTCTTTC TCTCTAAAAC TGAAAACGCA TCTGCTGTAA GCAAAGCCCC AGGAAAAGAT 
GAGAGTATTA GGGCTGCATG GGGTATTAAC ATCAAGAAAA AGGGAAAGAA AATGAGTCCA 
AAAACTTTTG GGGTGAGGCT GGAGGACTGT CTGCCTGCTG CCAGTAATAA GTTTGTTCCA 
CAGATTGTGG AGACTTGTTG TAGTCTGGTG GAAGAGATGG GTTTGGAATA TACTGGCATA 
TACAGAGTGC CGGGGAACAA TGTCATGGTG TCTTCTCTTC AGGAACAGCT CGACAAAGGC 
ATGGTTATAG ACACTGCTGA GGAGAGATGG AAAGACCTGA ATGTCATCAG CAGTCTTCTG 
AAATCCTTCT TCAGAAAGCT TCCAGAACCT CTTTTTACAG ATGATAAATA TAATGACTTC 
ATTGATGCCA ACCGTTTAGA GGATACAGGG GATCGCTTGA AGACCATGAG GAAACTTATA 
CGTGACCTGC CGGATCACAA CTACCACACA CTGAAGTTTC TTATCGGTCA TCTGAAAACA 
GTTGCGGACC ATTCAGAAAA GAATAAGATG GAGCCTAGAA ATCTGGCCCT GGTGTTTGGC 
CCAACACTGG TGCGGACCTC AGAAGACAAC ATGATCGAGA TGGTCACTCA CATGGCAGAC 
CGCTACAAAA TCACAGAGAC TCTTATCTTG CATCATACAT GGTTCTTCAG TGATGATCTG 
GATACGGATA AGACACCAGA GGATCAGAAG CAGAAGGAAA TGCAGCCAGT TCTAAATATT 
GACCACCTTC TGTCTAACAT TGGCAGGGTG GTGCCACTGC AAGACTTCTC AGCTGAGCGA 
ATGGATCAAA GTTTGCGATT CCACCAGCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_690730.6
CDS	596..3808
Translation	MDQGKDHKNEGLSSSQKTRPGETMAWKGPLTVVLRKNSLGFGFTLRHFIVYPPESAFNTNLKDEENGNGKGGQQFRFEPVDTIFVKNVKEEGPAHQAGLCTGDRLVRVNGESVLGKTFSQVIALIQNSDNVLELSIMPKDEDVLQLAYSQDAYLRGNEPYTGETQNLPDPPPVCNLNSKSPSTSQNHAIPVNMNSEIVVVSQPSPHCLIRHRICSSSSGGKTNLLDFHFANHNAAIASASLVHPRKASLPQGHSELCHQGLSDWYYSQVDQSSLGNSHHYQSSSQDRLYELGLAPGGSRGGRTHNSSQDALLHYSAVMGIHHSPCTDSYGLGRTSKLSSHSCSENLLAAYASYEQSFGHSLETLEKASILISPHYKIPTQPHEITQLIRVAECKSIANNHTNGSAVLQPSGEGLRQKQQPAVHIRHLPAQYVEGQMVGCHTSIPKTIPLQNPSSITPSAAESQEPQRSLVTVFTEEPLQLKKNLPSGIETHHCTSVNFIKPLSAKVLPSRSEYSVHKTNSGITPSSVEQDSLAVIPFKGSLKKSRRSSYMLAITTERSKSCDEGLNTFREEERVLLKLPKRVKSFFTDDSLESLKVAEDAHSKRHSTSELGNINLTDIRKQGWLHYKQILNDKRKKVGSGMRQWKRVFSVLHSSLLFLYKDKREAVLHATRAGSHGIGQEKDIQTISIQGCLIDIAYSETKRKHTFRLMTQDFCEYLLQAEDRDDMLMWIRVIREKSKTENEELGFSRQVLISKKLNDYKKQSPTSNKPDTSPKMHQMMPPFFLSKTENASAVSKAPGKDESIRAAWGINIKKKGKKMSPKTFGVRLEDCLPAASNKFVPQIVETCCSLVEEMGLEYTGIYRVPGNNVMVSSLQEQLDKGMVIDTAEERWKDLNVISSLLKSFFRKLPEPLFTDDKYNDFIDANRLEDTGDRLKTMRKLIRDLPDHNYHTLKFLIGHLKTVADHSEKNKMEPRNLALVFGPTLVRTSEDNMIEMVTHMADRYKITETLILHHTWFFSDDLDTDKTPEDQKQKEMQPVLNIDHLLSNIGRVVPLQDFSAERMDQSLRFHQP

Target ORF information:

RefSeq Version	XM_685638.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 23b (arhgap23b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_685638.8

ORF Insert Sequence:

ATGGATCAGG GTAAAGATCA CAAAAATGAA GGATTGTCGT CTAGTCAGAA GACTCGACCA 
GGAGAGACCA TGGCCTGGAA GGGTCCTCTC ACAGTGGTTC TGCGTAAAAA CTCTTTGGGC 
TTTGGTTTCA CATTGCGTCA TTTCATAGTC TATCCACCAG AGTCTGCCTT CAACACTAAC 
CTGAAGGATG AAGAAAATGG AAATGGCAAA GGAGGTCAGC AGTTTCGTTT TGAGCCAGTG 
GATACCATCT TTGTAAAGAA TGTGAAAGAG GAAGGACCCG CTCATCAGGC AGGCCTCTGC 
ACAGGTGACA GACTAGTGAG GGTGAATGGG GAAAGTGTGC TGGGGAAAAC GTTCTCTCAG 
GTCATAGCAT TGATTCAGAA TAGTGACAAT GTGCTGGAGT TGTCCATCAT GCCAAAAGAT 
GAGGATGTGC TACAGCTGGC ATATTCTCAA GATGCCTACT TGAGAGGGAA TGAGCCATAC 
ACTGGAGAAA CACAAAACCT TCCCGATCCT CCTCCAGTCT GTAACTTAAA CAGCAAATCT 
CCCTCAACAT CGCAGAACCA TGCTATTCCT GTTAACATGA ACTCTGAGAT TGTTGTAGTG 
TCTCAACCTT CCCCTCACTG CCTGATTCGT CACCGCATCT GCTCTTCATC CTCAGGTGGG 
AAAACAAACC TGCTGGACTT CCACTTTGCC AACCACAATG CAGCTATTGC CTCTGCCTCA 
CTAGTTCACC CTAGGAAAGC CAGTCTTCCC CAAGGTCACA GTGAACTGTG CCACCAAGGT 
CTGTCAGACT GGTACTATAG CCAAGTAGAC CAATCAAGCC TTGGCAATTC CCATCATTAC 
CAGAGCAGCT CTCAAGACAG GCTTTATGAA CTGGGACTGG CACCAGGAGG GTCTCGTGGT 
GGTCGGACAC ACAACTCATC TCAGGATGCC CTACTACATT ACAGTGCAGT GATGGGGATC 
CACCATTCAC CTTGCACAGA TTCTTATGGT CTTGGGAGAA CAAGTAAACT CTCTAGCCAT 
TCCTGTTCTG AAAACTTGCT AGCTGCTTAT GCTTCGTACG AGCAGAGCTT TGGTCATTCA 
CTGGAAACTC TAGAAAAGGC ATCCATTCTG ATCTCACCCC ATTATAAAAT ACCAACACAG 
CCTCATGAAA TCACTCAGCT AATCAGGGTA GCGGAGTGCA AAAGTATTGC CAATAATCAC 
ACAAATGGTT CCGCAGTTTT GCAACCATCA GGTGAGGGTT TAAGGCAAAA ACAGCAGCCA 
GCTGTTCACA TCAGACATTT ACCTGCACAA TATGTTGAGG GTCAGATGGT TGGCTGCCAC 
ACCTCCATTC CAAAAACCAT CCCACTGCAG AATCCATCCT CCATCACTCC CTCTGCTGCA 
GAGTCCCAAG AGCCCCAACG CTCACTGGTC ACTGTGTTTA CTGAGGAACC TTTACAGCTT 
AAAAAAAATC TCCCCTCAGG AATTGAGACA CACCATTGTA CTTCTGTGAA TTTTATTAAA 
CCCCTTTCAG CTAAAGTTCT TCCCTCAAGG TCAGAATATT CTGTACACAA AACTAATAGT 
GGCATTACAC CCTCATCTGT GGAGCAGGAC TCCTTGGCTG TCATCCCATT CAAAGGCAGT 
CTGAAAAAAA GCCGTCGCTC CTCCTACATG CTGGCCATTA CTACGGAGCG ATCTAAATCA 
TGTGACGAGG GTCTGAACAC TTTTAGGGAA GAAGAACGTG TTCTCTTGAA ACTGCCAAAA 
AGAGTCAAAA GCTTTTTTAC AGATGATTCT CTAGAGAGTC TTAAAGTGGC AGAAGATGCA 
CACTCTAAAC GCCACTCCAC TTCTGAACTG GGAAACATCA ATCTTACAGA CATCAGAAAA 
CAAGGCTGGC TGCATTATAA ACAGATTCTT AATGATAAAC GGAAGAAAGT GGGCAGTGGT 
ATGCGGCAGT GGAAAAGAGT ATTTTCTGTG CTGCACTCTA GTTTACTGTT CCTCTACAAG 
GATAAGCGTG AGGCGGTGCT TCATGCAACT AGGGCAGGAA GTCATGGCAT TGGGCAAGAG 
AAGGATATTC AAACAATCAG CATTCAAGGC TGCTTGATTG ACATTGCATA CAGCGAGACT 
AAGCGTAAAC ACACTTTTCG GCTGATGACT CAGGACTTCT GTGAGTATCT TCTGCAGGCA 
GAGGACAGAG ATGACATGCT GATGTGGATA AGAGTTATCA GAGAGAAAAG CAAGACCGAA 
AATGAGGAAC TGGGCTTCTC CAGACAGGTT CTCATCAGTA AGAAGCTGAA TGACTATAAG 
AAACAGAGTC CAACAAGCAA CAAGCCAGAT ACTTCTCCTA AGATGCATCA AATGATGCCA 
CCTTTCTTTC TCTCTAAAAC TGAAAACGCA TCTGCTGTAA GCAAAGCCCC AGGAAAAGAT 
GAGAGTATTA GGGCTGCATG GGGTATTAAC ATCAAGAAAA AGGGAAAGAA AATGAGTCCA 
AAAACTTTTG GGGTGAGGCT GGAGGACTGT CTGCCTGCTG CCAGTAATAA GTTTGTTCCA 
CAGATTGTGG AGACTTGTTG TAGTCTGGTG GAAGAGATGG GTTTGGAATA TACTGGCATA 
TACAGAGTGC CGGGGAACAA TGTCATGGTG TCTTCTCTTC AGGAACAGCT CGACAAAGGC 
ATGGTTATAG ACACTGCTGA GGAGAGATGG AAAGACCTGA ATGTCATCAG CAGTCTTCTG 
AAATCCTTCT TCAGAAAGCT TCCAGAACCT CTTTTTACAG ATGATAAATA TAATGACTTC 
ATTGATGCCA ACCGTTTAGA GGATACAGGG GATCGCTTGA AGACCATGAG GAAACTTATA 
CGTGACCTGC CGGATCACAA CTACCACACA CTGAAGTTTC TTATCGGTCA TCTGAAAACA 
GTTGCGGACC ATTCAGAAAA GAATAAGATG GAGCCTAGAA ATCTGGCCCT GGTGTTTGGC 
CCAACACTGG TGCGGACCTC AGAAGACAAC ATGATCGAGA TGGTCACTCA CATGGCAGAC 
CGCTACAAAA TCACAGAGAC TCTTATCTTG CATCATACAT GGTTCTTCAG TGATGATCTG 
GATACGGATA AGACACCAGA GGATCAGAAG CAGAAGGAAA TGCAGCCAGT TCTAAATATT 
GACCACCTTC TGTCTAACAT TGGCAGGGTG GTGCCACTGC AAGACTTCTC AGCTGAGCGA 
ATGGATCAAA GTTTGCGATT CCACCAGCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap23b ( XM_685638.9 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_690730.6 Danio rerio Rho GTPase activating protein 23b (arhgap23b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17719
Clone ID Related Accession (Same CDS sequence)	XM_685638.8 , XM_685638.9
Accession Version	XM_685638.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_685638.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAGG GTAAAGATCA CAAAAATGAA GGATTGTCGT CTAGTCAGAA GACTCGACCA 
GGAGAGACCA TGGCCTGGAA GGGTCCTCTC ACAGTGGTTC TGCGTAAAAA CTCTTTGGGC 
TTTGGTTTCA CATTGCGTCA TTTCATAGTC TATCCACCAG AGTCTGCCTT CAACACTAAC 
CTGAAGGATG AAGAAAATGG AAATGGCAAA GGAGGTCAGC AGTTTCGTTT TGAGCCAGTG 
GATACCATCT TTGTAAAGAA TGTGAAAGAG GAAGGACCCG CTCATCAGGC AGGCCTCTGC 
ACAGGTGACA GACTAGTGAG GGTGAATGGG GAAAGTGTGC TGGGGAAAAC GTTCTCTCAG 
GTCATAGCAT TGATTCAGAA TAGTGACAAT GTGCTGGAGT TGTCCATCAT GCCAAAAGAT 
GAGGATGTGC TACAGCTGGC ATATTCTCAA GATGCCTACT TGAGAGGGAA TGAGCCATAC 
ACTGGAGAAA CACAAAACCT TCCCGATCCT CCTCCAGTCT GTAACTTAAA CAGCAAATCT 
CCCTCAACAT CGCAGAACCA TGCTATTCCT GTTAACATGA ACTCTGAGAT TGTTGTAGTG 
TCTCAACCTT CCCCTCACTG CCTGATTCGT CACCGCATCT GCTCTTCATC CTCAGGTGGG 
AAAACAAACC TGCTGGACTT CCACTTTGCC AACCACAATG CAGCTATTGC CTCTGCCTCA 
CTAGTTCACC CTAGGAAAGC CAGTCTTCCC CAAGGTCACA GTGAACTGTG CCACCAAGGT 
CTGTCAGACT GGTACTATAG CCAAGTAGAC CAATCAAGCC TTGGCAATTC CCATCATTAC 
CAGAGCAGCT CTCAAGACAG GCTTTATGAA CTGGGACTGG CACCAGGAGG GTCTCGTGGT 
GGTCGGACAC ACAACTCATC TCAGGATGCC CTACTACATT ACAGTGCAGT GATGGGGATC 
CACCATTCAC CTTGCACAGA TTCTTATGGT CTTGGGAGAA CAAGTAAACT CTCTAGCCAT 
TCCTGTTCTG AAAACTTGCT AGCTGCTTAT GCTTCGTACG AGCAGAGCTT TGGTCATTCA 
CTGGAAACTC TAGAAAAGGC ATCCATTCTG ATCTCACCCC ATTATAAAAT ACCAACACAG 
CCTCATGAAA TCACTCAGCT AATCAGGGTA GCGGAGTGCA AAAGTATTGC CAATAATCAC 
ACAAATGGTT CCGCAGTTTT GCAACCATCA GGTGAGGGTT TAAGGCAAAA ACAGCAGCCA 
GCTGTTCACA TCAGACATTT ACCTGCACAA TATGTTGAGG GTCAGATGGT TGGCTGCCAC 
ACCTCCATTC CAAAAACCAT CCCACTGCAG AATCCATCCT CCATCACTCC CTCTGCTGCA 
GAGTCCCAAG AGCCCCAACG CTCACTGGTC ACTGTGTTTA CTGAGGAACC TTTACAGCTT 
AAAAAAAATC TCCCCTCAGG AATTGAGACA CACCATTGTA CTTCTGTGAA TTTTATTAAA 
CCCCTTTCAG CTAAAGTTCT TCCCTCAAGG TCAGAATATT CTGTACACAA AACTAATAGT 
GGCATTACAC CCTCATCTGT GGAGCAGGAC TCCTTGGCTG TCATCCCATT CAAAGGCAGT 
CTGAAAAAAA GCCGTCGCTC CTCCTACATG CTGGCCATTA CTACGGAGCG ATCTAAATCA 
TGTGACGAGG GTCTGAACAC TTTTAGGGAA GAAGAACGTG TTCTCTTGAA ACTGCCAAAA 
AGAGTCAAAA GCTTTTTTAC AGATGATTCT CTAGAGAGTC TTAAAGTGGC AGAAGATGCA 
CACTCTAAAC GCCACTCCAC TTCTGAACTG GGAAACATCA ATCTTACAGA CATCAGAAAA 
CAAGGCTGGC TGCATTATAA ACAGATTCTT AATGATAAAC GGAAGAAAGT GGGCAGTGGT 
ATGCGGCAGT GGAAAAGAGT ATTTTCTGTG CTGCACTCTA GTTTACTGTT CCTCTACAAG 
GATAAGCGTG AGGCGGTGCT TCATGCAACT AGGGCAGGAA GTCATGGCAT TGGGCAAGAG 
AAGGATATTC AAACAATCAG CATTCAAGGC TGCTTGATTG ACATTGCATA CAGCGAGACT 
AAGCGTAAAC ACACTTTTCG GCTGATGACT CAGGACTTCT GTGAGTATCT TCTGCAGGCA 
GAGGACAGAG ATGACATGCT GATGTGGATA AGAGTTATCA GAGAGAAAAG CAAGACCGAA 
AATGAGGAAC TGGGCTTCTC CAGACAGGTT CTCATCAGTA AGAAGCTGAA TGACTATAAG 
AAACAGAGTC CAACAAGCAA CAAGCCAGAT ACTTCTCCTA AGATGCATCA AATGATGCCA 
CCTTTCTTTC TCTCTAAAAC TGAAAACGCA TCTGCTGTAA GCAAAGCCCC AGGAAAAGAT 
GAGAGTATTA GGGCTGCATG GGGTATTAAC ATCAAGAAAA AGGGAAAGAA AATGAGTCCA 
AAAACTTTTG GGGTGAGGCT GGAGGACTGT CTGCCTGCTG CCAGTAATAA GTTTGTTCCA 
CAGATTGTGG AGACTTGTTG TAGTCTGGTG GAAGAGATGG GTTTGGAATA TACTGGCATA 
TACAGAGTGC CGGGGAACAA TGTCATGGTG TCTTCTCTTC AGGAACAGCT CGACAAAGGC 
ATGGTTATAG ACACTGCTGA GGAGAGATGG AAAGACCTGA ATGTCATCAG CAGTCTTCTG 
AAATCCTTCT TCAGAAAGCT TCCAGAACCT CTTTTTACAG ATGATAAATA TAATGACTTC 
ATTGATGCCA ACCGTTTAGA GGATACAGGG GATCGCTTGA AGACCATGAG GAAACTTATA 
CGTGACCTGC CGGATCACAA CTACCACACA CTGAAGTTTC TTATCGGTCA TCTGAAAACA 
GTTGCGGACC ATTCAGAAAA GAATAAGATG GAGCCTAGAA ATCTGGCCCT GGTGTTTGGC 
CCAACACTGG TGCGGACCTC AGAAGACAAC ATGATCGAGA TGGTCACTCA CATGGCAGAC 
CGCTACAAAA TCACAGAGAC TCTTATCTTG CATCATACAT GGTTCTTCAG TGATGATCTG 
GATACGGATA AGACACCAGA GGATCAGAAG CAGAAGGAAA TGCAGCCAGT TCTAAATATT 
GACCACCTTC TGTCTAACAT TGGCAGGGTG GTGCCACTGC AAGACTTCTC AGCTGAGCGA 
ATGGATCAAA GTTTGCGATT CCACCAGCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_690730.6
CDS	630..3842
Translation	MDQGKDHKNEGLSSSQKTRPGETMAWKGPLTVVLRKNSLGFGFTLRHFIVYPPESAFNTNLKDEENGNGKGGQQFRFEPVDTIFVKNVKEEGPAHQAGLCTGDRLVRVNGESVLGKTFSQVIALIQNSDNVLELSIMPKDEDVLQLAYSQDAYLRGNEPYTGETQNLPDPPPVCNLNSKSPSTSQNHAIPVNMNSEIVVVSQPSPHCLIRHRICSSSSGGKTNLLDFHFANHNAAIASASLVHPRKASLPQGHSELCHQGLSDWYYSQVDQSSLGNSHHYQSSSQDRLYELGLAPGGSRGGRTHNSSQDALLHYSAVMGIHHSPCTDSYGLGRTSKLSSHSCSENLLAAYASYEQSFGHSLETLEKASILISPHYKIPTQPHEITQLIRVAECKSIANNHTNGSAVLQPSGEGLRQKQQPAVHIRHLPAQYVEGQMVGCHTSIPKTIPLQNPSSITPSAAESQEPQRSLVTVFTEEPLQLKKNLPSGIETHHCTSVNFIKPLSAKVLPSRSEYSVHKTNSGITPSSVEQDSLAVIPFKGSLKKSRRSSYMLAITTERSKSCDEGLNTFREEERVLLKLPKRVKSFFTDDSLESLKVAEDAHSKRHSTSELGNINLTDIRKQGWLHYKQILNDKRKKVGSGMRQWKRVFSVLHSSLLFLYKDKREAVLHATRAGSHGIGQEKDIQTISIQGCLIDIAYSETKRKHTFRLMTQDFCEYLLQAEDRDDMLMWIRVIREKSKTENEELGFSRQVLISKKLNDYKKQSPTSNKPDTSPKMHQMMPPFFLSKTENASAVSKAPGKDESIRAAWGINIKKKGKKMSPKTFGVRLEDCLPAASNKFVPQIVETCCSLVEEMGLEYTGIYRVPGNNVMVSSLQEQLDKGMVIDTAEERWKDLNVISSLLKSFFRKLPEPLFTDDKYNDFIDANRLEDTGDRLKTMRKLIRDLPDHNYHTLKFLIGHLKTVADHSEKNKMEPRNLALVFGPTLVRTSEDNMIEMVTHMADRYKITETLILHHTWFFSDDLDTDKTPEDQKQKEMQPVLNIDHLLSNIGRVVPLQDFSAERMDQSLRFHQP

Target ORF information:

RefSeq Version	XM_685638.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 23b (arhgap23b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_685638.9

ORF Insert Sequence:

ATGGATCAGG GTAAAGATCA CAAAAATGAA GGATTGTCGT CTAGTCAGAA GACTCGACCA 
GGAGAGACCA TGGCCTGGAA GGGTCCTCTC ACAGTGGTTC TGCGTAAAAA CTCTTTGGGC 
TTTGGTTTCA CATTGCGTCA TTTCATAGTC TATCCACCAG AGTCTGCCTT CAACACTAAC 
CTGAAGGATG AAGAAAATGG AAATGGCAAA GGAGGTCAGC AGTTTCGTTT TGAGCCAGTG 
GATACCATCT TTGTAAAGAA TGTGAAAGAG GAAGGACCCG CTCATCAGGC AGGCCTCTGC 
ACAGGTGACA GACTAGTGAG GGTGAATGGG GAAAGTGTGC TGGGGAAAAC GTTCTCTCAG 
GTCATAGCAT TGATTCAGAA TAGTGACAAT GTGCTGGAGT TGTCCATCAT GCCAAAAGAT 
GAGGATGTGC TACAGCTGGC ATATTCTCAA GATGCCTACT TGAGAGGGAA TGAGCCATAC 
ACTGGAGAAA CACAAAACCT TCCCGATCCT CCTCCAGTCT GTAACTTAAA CAGCAAATCT 
CCCTCAACAT CGCAGAACCA TGCTATTCCT GTTAACATGA ACTCTGAGAT TGTTGTAGTG 
TCTCAACCTT CCCCTCACTG CCTGATTCGT CACCGCATCT GCTCTTCATC CTCAGGTGGG 
AAAACAAACC TGCTGGACTT CCACTTTGCC AACCACAATG CAGCTATTGC CTCTGCCTCA 
CTAGTTCACC CTAGGAAAGC CAGTCTTCCC CAAGGTCACA GTGAACTGTG CCACCAAGGT 
CTGTCAGACT GGTACTATAG CCAAGTAGAC CAATCAAGCC TTGGCAATTC CCATCATTAC 
CAGAGCAGCT CTCAAGACAG GCTTTATGAA CTGGGACTGG CACCAGGAGG GTCTCGTGGT 
GGTCGGACAC ACAACTCATC TCAGGATGCC CTACTACATT ACAGTGCAGT GATGGGGATC 
CACCATTCAC CTTGCACAGA TTCTTATGGT CTTGGGAGAA CAAGTAAACT CTCTAGCCAT 
TCCTGTTCTG AAAACTTGCT AGCTGCTTAT GCTTCGTACG AGCAGAGCTT TGGTCATTCA 
CTGGAAACTC TAGAAAAGGC ATCCATTCTG ATCTCACCCC ATTATAAAAT ACCAACACAG 
CCTCATGAAA TCACTCAGCT AATCAGGGTA GCGGAGTGCA AAAGTATTGC CAATAATCAC 
ACAAATGGTT CCGCAGTTTT GCAACCATCA GGTGAGGGTT TAAGGCAAAA ACAGCAGCCA 
GCTGTTCACA TCAGACATTT ACCTGCACAA TATGTTGAGG GTCAGATGGT TGGCTGCCAC 
ACCTCCATTC CAAAAACCAT CCCACTGCAG AATCCATCCT CCATCACTCC CTCTGCTGCA 
GAGTCCCAAG AGCCCCAACG CTCACTGGTC ACTGTGTTTA CTGAGGAACC TTTACAGCTT 
AAAAAAAATC TCCCCTCAGG AATTGAGACA CACCATTGTA CTTCTGTGAA TTTTATTAAA 
CCCCTTTCAG CTAAAGTTCT TCCCTCAAGG TCAGAATATT CTGTACACAA AACTAATAGT 
GGCATTACAC CCTCATCTGT GGAGCAGGAC TCCTTGGCTG TCATCCCATT CAAAGGCAGT 
CTGAAAAAAA GCCGTCGCTC CTCCTACATG CTGGCCATTA CTACGGAGCG ATCTAAATCA 
TGTGACGAGG GTCTGAACAC TTTTAGGGAA GAAGAACGTG TTCTCTTGAA ACTGCCAAAA 
AGAGTCAAAA GCTTTTTTAC AGATGATTCT CTAGAGAGTC TTAAAGTGGC AGAAGATGCA 
CACTCTAAAC GCCACTCCAC TTCTGAACTG GGAAACATCA ATCTTACAGA CATCAGAAAA 
CAAGGCTGGC TGCATTATAA ACAGATTCTT AATGATAAAC GGAAGAAAGT GGGCAGTGGT 
ATGCGGCAGT GGAAAAGAGT ATTTTCTGTG CTGCACTCTA GTTTACTGTT CCTCTACAAG 
GATAAGCGTG AGGCGGTGCT TCATGCAACT AGGGCAGGAA GTCATGGCAT TGGGCAAGAG 
AAGGATATTC AAACAATCAG CATTCAAGGC TGCTTGATTG ACATTGCATA CAGCGAGACT 
AAGCGTAAAC ACACTTTTCG GCTGATGACT CAGGACTTCT GTGAGTATCT TCTGCAGGCA 
GAGGACAGAG ATGACATGCT GATGTGGATA AGAGTTATCA GAGAGAAAAG CAAGACCGAA 
AATGAGGAAC TGGGCTTCTC CAGACAGGTT CTCATCAGTA AGAAGCTGAA TGACTATAAG 
AAACAGAGTC CAACAAGCAA CAAGCCAGAT ACTTCTCCTA AGATGCATCA AATGATGCCA 
CCTTTCTTTC TCTCTAAAAC TGAAAACGCA TCTGCTGTAA GCAAAGCCCC AGGAAAAGAT 
GAGAGTATTA GGGCTGCATG GGGTATTAAC ATCAAGAAAA AGGGAAAGAA AATGAGTCCA 
AAAACTTTTG GGGTGAGGCT GGAGGACTGT CTGCCTGCTG CCAGTAATAA GTTTGTTCCA 
CAGATTGTGG AGACTTGTTG TAGTCTGGTG GAAGAGATGG GTTTGGAATA TACTGGCATA 
TACAGAGTGC CGGGGAACAA TGTCATGGTG TCTTCTCTTC AGGAACAGCT CGACAAAGGC 
ATGGTTATAG ACACTGCTGA GGAGAGATGG AAAGACCTGA ATGTCATCAG CAGTCTTCTG 
AAATCCTTCT TCAGAAAGCT TCCAGAACCT CTTTTTACAG ATGATAAATA TAATGACTTC 
ATTGATGCCA ACCGTTTAGA GGATACAGGG GATCGCTTGA AGACCATGAG GAAACTTATA 
CGTGACCTGC CGGATCACAA CTACCACACA CTGAAGTTTC TTATCGGTCA TCTGAAAACA 
GTTGCGGACC ATTCAGAAAA GAATAAGATG GAGCCTAGAA ATCTGGCCCT GGTGTTTGGC 
CCAACACTGG TGCGGACCTC AGAAGACAAC ATGATCGAGA TGGTCACTCA CATGGCAGAC 
CGCTACAAAA TCACAGAGAC TCTTATCTTG CATCATACAT GGTTCTTCAG TGATGATCTG 
GATACGGATA AGACACCAGA GGATCAGAAG CAGAAGGAAA TGCAGCCAGT TCTAAATATT 
GACCACCTTC TGTCTAACAT TGGCAGGGTG GTGCCACTGC AAGACTTCTC AGCTGAGCGA 
ATGGATCAAA GTTTGCGATT CCACCAGCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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arhgap23b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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