crb1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	crb1
Entrez Gene ID	560942
Full Name	crumbs family member 1, photoreceptor morphogenesis associated
Synonyms	si:dkey-114d20.6,si:dkey-114d20.7
General protein information	Preferred Names crumbs family member 1, photoreceptor morphogenesis associated Names protein crumbs homolog 1 crumbs homolog 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001044943.1	NP_001038408.1	protein crumbs homolog 1
XM_005161738.3	XP_005161795.1	protein crumbs homolog 1 isoform X1
XM_005161738.4	XP_005161795.1	protein crumbs homolog 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CRB1	XP_005622350.1
Mus musculus (house mouse)	Crb1	NP_573502.2
Gallus gallus (chicken)	CRB1	XP_003641718.2
Pan troglodytes (chimpanzee)	CRB1	XP_525009.2
Danio rerio (zebrafish)	crb1	NP_001038408.1
Xenopus tropicalis (tropical clawed frog)	crb1	XP_002932223.2
Homo sapiens (human)	CRB1	NP_957705.1
Macaca mulatta (Rhesus monkey)	CRB1	XP_001110912.1
Bos taurus (cattle)	CRB1	NP_001179411.1
Rattus norvegicus (Norway rat)	Crb1	NP_001100652.1

Gene Ontology
Bibliography

Related articles in PubMed

Notch-mediated inhibition of neurogenesis is required for zebrafish spinal cord morphogenesis.
Sharma P, Saraswathy VM, Xiang L, Fürthauer M
Scientific reports9(1)9958(2019 Jul)

Regulation of the apical extension morphogenesis tunes the mechanosensory response of microvilliated neurons.
Desban L, Prendergast A, Roussel J, Rosello M, Geny D, Wyart C, Bardet PL
PLoS biology17(4)e3000235(2019 04)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Spatial-temporal expressions of Crumbs and Nagie oko and their interdependence in zebrafish central nervous system during early development.
Zou J, Wen Y, Yang X, Wei X
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience31(8)770-82(2013 Dec)

Crb apical polarity proteins maintain zebrafish retinal cone mosaics via intercellular binding of their extracellular domains.
Zou J, Wang X, Wei X
Developmental cell22(6)1261-74(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following crb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03024	NM_001044943.1 Latest version!	Danio rerio crumbs cell polarity complex component 1 (crb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa27000	XM_005161738.4 Latest version!	Danio rerio crumbs family member 1, photoreceptor morphogenesis associated (crb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa27000	XM_005161738.3	Danio rerio crumbs family member 1, photoreceptor morphogenesis associated (crb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crb1 ( NM_001044943.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038408.1 Danio rerio crumbs cell polarity complex component 1 (crb1), mRNA.

Price & Availability↑

CloneID	ODa03024
Clone ID Related Accession (Same CDS sequence)	NM_001044943.1
Accession Version	NM_001044943.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574438400000
Organism	Danio rerio(zebrafish)
Product	protein crumbs homolog 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ314735.1. On Sep 1, 2006 this sequence version replaced XM_684191.1. ##Evidence-Data-START## Transcript exon combination :: DQ314735.1, BC163555.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGATATGG ATCTAGCGGT TTGGGGTTCA GTGTGGCTCA GATTGGTGGC TTGTGTCTTG 
TTTGTTCTGC TGCAATGGAC AGAAAGTGTA ACACTACTCA AGACCTCAAC ACTCTGCCTG 
GACAACCCTT GCCAACACCA GTCAGAGTGC AGGGAAGCCC TGTCTGATTT CCTGTGCCAA 
TGTCAGACGA CTGTGCCAGT CTTTCCCAGC ACGCGTTGTG ATTCTTCCAG TACGCTCTGC 
CAACTGTCCA TCTGCCAGGG CAATGCGACA TGTCAACCCA CCGGCGCCCA CCCAGGGGAA 
CTAGTTTGCC AGTGTGACTC CGGCTTGTTG GGGCAGGACT GTCTTTCCAG TGCCCAGCTC 
TGCGCTCAGG GGCATTGTGG GGACAGTGCC CACTGTCTGG CAGTGCGAGA CCAGAGTCCA 
GGGTACGCAT GCATCTGTCA GGAGGGTTAC ACTGGGAGAT CTTGCGAGAA AGAGGTGGAC 
CACTGCTCTC CTAACCCCTG CCGAAACCGC GCCATCTGCC GCAGCAGAAG GAATGGGCCC 
ACTTGCTTCT GCGTGCCAGG ATTCCAGGGC CAGCTCTGCG AGATTGAGGT GAATGAATGT 
GTGTCGAGGC CCTGTAGGAA CGGAGCCACC TGTGTGGACA AGATCGGGCA CTACATCTGT 
CTCTGCAGGC CTGGATACAT GGGTAGCAGC TGTGAGCTGG AGATTGATGA GTGTCAGTCA 
CAGCCCTGTC TCCACGGCGC CAGTTGCCAT GACCACATCA ATGGCTTCAC GTGCACATGC 
CTGGCGGGTT TCCAGGGCGA GAGCTGTGAA ATCAACATCG ACGAGTGCCG TGACCAGCCA 
TGCCAAAACG GGGCTCTGTG TGTGGATGAA ATCAATAGCT ACCGCTGTGA CTGCTCACAG 
ACTGCTAACT TCACTGGTGT TGACTGTGAG ATCCCGCCTC CGCCCTGCTG GTCTCAACCC 
TGCCTCAACA GCGCCCTCTG TGAGGATCAA CAGGAGAACT ACACCTGCAA CTGCTGGCCA 
GGGTTTGAGG GGCGTAACTG TGAGGTAGAC GTCAGTGAAT GTGAAAGCAG TCCCTGTGTG 
AATGAAGGCA TTTGCATGGA GCTTTCTTGG AAAACACTTT ACGGTACTGA GCCCCTGTTT 
ACAGCTAGAT ACAATCCCAG ACTTGCTTCC GGGTTCATCT GCAAGTGTCC GCCAGGATTC 
TCAGGTGCCT TGTGCGAGCA AAACACAACT GCCTGTACCA CCAGCCCATG TCATAATGGA 
GCCACATGTG AAGACTTCCT AGGCAGCTAC AAGTGCATCT GCCCTTCTGA AAGCGAAGAT 
GGAGTTCTCT ACGGTGGTCG AAACTGTAGC GAGCCTCTTA CAGGTTGCGA AGGTCACGAG 
TGCCAGAACG GAGCGTCCTG CATCCCATTC CTCAGCGAAG GTGTACATGG ATACAGTTGC 
ATCTGTCAGC CTGGCTACAC CGGCTCTTAC TGCCAAACGC TCACTGTGTT CTCCTTTGAG 
ACAAGCAGAG CATTCTTGCA CCTGCAGACC CCGCTGCTGG GTGCAGAAAC ATACTTCAAT 
ATCACGCTAA GCTTTCGTAC GGTCCTGGAG AACACAGTGC TCTTCCAAAG GGGCAGCGGA 
GGGGTCACAC TCAGCCTGGA GATACAGGAG ACACACCTGA TTCTTGATCT TAAGACTGAC 
CCTCAGCCAA ACGCAACCAG CTGGACTCTG ATGTTGCCTC AGGTTGTATC TGATGGTGAA 
TGGCACACAG TAGAAGCCGT ACTAGGAGAG GGTACCCTGC TGCTGCAGCT GCTGGAGCCA 
TGCCAGGGTG GACAAAACTG TGGTACAACA GCCCAAGTAA AGATTGGCGC CCTTGAGCTG 
GAGTCAGCCC TACTAAGCAC CTTTGTTGGA GGATTGGATG AGGGAGGGAG CTCCAGATCA 
TTCATCGGCT GCATGAGGGA CTTGCTCGTG GACTCGCAGC TGATGATACC GGAGGACTGG 
TTGAGCAGCT CAGCTGTAAA TGTTGTGCAG GGCTGTAGCC ATCATGACCG ATGCCTTTCT 
GGTCCATGCG AGAACCATGG AGAATGTGTT AACCTTTGGC AAGGTTACCA GTGCAAATGT 
CTGCGCCCAT ATGTGGGTCA AAACTGTGAT GAGGAGTATA TTACCACCAG ATTTGGCCAG 
GAAGACTCGA CAAGCTATGC TGTCTTCACC ATCAATGATG AACCTGACTC GGAAACACTT 
CATCTGTCCA TGTTCTTGCG TACTCGAAAG GACTCTGGTC TTTTGGTGCT GCTGGCCAAC 
AGCACCTCTG ACTACTTACA GATGTGGCTG GAAAAGGGAA AACTGACAGT TCAAGTCAAC 
AACTTGAAGA CTGTGACTGG TGAAAGGGTG CTTAATGATG GTGAGAGACA TTTTGTCAGT 
GTCACCATCG AGGATGGCAT GATGACTCTG CAGAACTCGG ATCATGAATT CGAAGCAACA 
TATGTTCAAC CTGTTTCCAT TCAGTTTGGG GATACGATCA ATGTCGGTGG CCTGTTTGAT 
GTACAAGCCT TAACTGCATT TGGTGGACAT TTTAAAGGGT GTCTACAAGA CTTGCAGCTT 
AATGAGAAGA AGCTTGAGTT TTTCCCAATT GATACTTCTG CGATGTCGTA CAAACCCGAT 
CGCGTGGTGG AAGTTACTGC AGGCTGCACC AGTGATGACA CTTGCTCTAA AAATCCTTGC 
CAAAATGGTG GCATCTGCTT TTCTTCATGG GATGATTTTA CTTGCAACTG CCCTCCTAAC 
ACATCAGGAC AACACTGTGA GGAGGTGAGC TGGTGTGACC TCAACCCGTG CCCACCACAA 
GCAATATGCA AGGCCCTAAA CCAGGGCTAT GAATGCATTT CAAACGTGAC TTTCCAGGAG 
AACACCACAC TTGTATACCA AGGCAATGGG CTGATATCCC GCCATCTGAC CAGTATTGTC 
TTTAATATCC GTACACGTAA GCGCAATGCC ATAGTGTTGC ATGCAGAGAG TGGATCAGAG 
TTTGTAACTG TATCTCTTCA AGATGGATTT CTGGTTCTTG AGCTTCTAAG TGGACCTACT 
ACTTCATCAT CATTGTCACC AGTAACCCTG CACAGCCCAA GGGTAGTGGC TGATGGGGAA 
TGGCATGTTA TAGAGCTGTT AATGGCCACT CCAGGGTCTA ACAGCTCCCA CTGGATTATG 
GTCCCCCTAG ATGAAAAAGA TGAGCCAACT AAATCAGACT CTATGACCGG AAATCTAGAC 
TTCCTCAGAG AGAGTGTGTA CATCATGCTA GGAGGACTAG GCCCTGATTC TGGCTCCAAT 
CTAATTGGTT GCTTGAGTAA TGTTGAGATT GGTGGAATCG TGCTTCCGTA TTATGGACAG 
ACTGAGGTGA GATTCCCACG CACACAGGAA GAAATGTTCA ATAAGATATC AGAGGAGCCT 
GTTCAAACCG GCTGTTTCGG GGAGGTGGTG TGTGAGCCCA ATCCCTGCCT GCATGGAGGC 
ATCTGTGACG ATCACTTCAA CCTTTTTCAC TGCTTCTGTC TTCCTGGCTG GGGCGGTGAT 
CACTGTGAGC TGAATACAAA CACCTGCGCC TCCAACCCCT GTCGACATGG ATATTGTAGT 
GTGCAGGACC TCACATACAA CTGCACATGT GAGGACGGAT ACACTGGCAC CAACTGTGAG 
ATGAAGGTGG ATGCTTGTGC TGGGCACAGG TGTGCTAATG GAGCGACCTG CTTGCGTGGC 
TTCAACATGT ATTCCTGCCT CTGTACCGAC AAGTTTACTG GGCCACTTTG CAACATTCAG 
ATTGAAGAGG TTCCGTGGTA CGTAGTTGTC AGGAACATAC CGCCAAAGCT CCCGGTGTCC 
ATCTGTGGCG ATGAACAGCA GAACTACACA TGTTTCAATG GGGGGAACTG TTCTGACACT 
AACATGCCGT GCGACTGCCA CCCTGGGTTT TCGGGTCACT GGTGTGAACT AGAACTTGAT 
GAATGTAGAT CCAACCCTTG CAAGAATGGC GGCTACTGCC AGAACATGGT CAACAGGTTC 
CAATGCGTCT GTGAAATGAC TTTTGCCGGC GAGACCTGCG AAGTGGACCT GAACGCAGAG 
AGTGTGACGT CTCAGCTGTT GCTCTCCATC AGCCTGGTGT GTGTGGTTCT GCTCTTGGTT 
GTTTTTGGTG TGACCACAGC TCTGGTCATT GCACTGAACC GAAGGGCCAC ACGGGGCACT 
TACAGCCCCA GTCGGCAGGA GAAGGAAGGC TCAAGGGTGG AAATGTGGAA CATTGTACAA 
CCTCCACCAA TGGAGAGACT CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038408.1
CDS	75..4361
Translation	MDMDLAVWGSVWLRLVACVLFVLLQWTESVTLLKTSTLCLDNPCQHQSECREALSDFLCQCQTTVPVFPSTRCDSSSTLCQLSICQGNATCQPTGAHPGELVCQCDSGLLGQDCLSSAQLCAQGHCGDSAHCLAVRDQSPGYACICQEGYTGRSCEKEVDHCSPNPCRNRAICRSRRNGPTCFCVPGFQGQLCEIEVNECVSRPCRNGATCVDKIGHYICLCRPGYMGSSCELEIDECQSQPCLHGASCHDHINGFTCTCLAGFQGESCEINIDECRDQPCQNGALCVDEINSYRCDCSQTANFTGVDCEIPPPPCWSQPCLNSALCEDQQENYTCNCWPGFEGRNCEVDVSECESSPCVNEGICMELSWKTLYGTEPLFTARYNPRLASGFICKCPPGFSGALCEQNTTACTTSPCHNGATCEDFLGSYKCICPSESEDGVLYGGRNCSEPLTGCEGHECQNGASCIPFLSEGVHGYSCICQPGYTGSYCQTLTVFSFETSRAFLHLQTPLLGAETYFNITLSFRTVLENTVLFQRGSGGVTLSLEIQETHLILDLKTDPQPNATSWTLMLPQVVSDGEWHTVEAVLGEGTLLLQLLEPCQGGQNCGTTAQVKIGALELESALLSTFVGGLDEGGSSRSFIGCMRDLLVDSQLMIPEDWLSSSAVNVVQGCSHHDRCLSGPCENHGECVNLWQGYQCKCLRPYVGQNCDEEYITTRFGQEDSTSYAVFTINDEPDSETLHLSMFLRTRKDSGLLVLLANSTSDYLQMWLEKGKLTVQVNNLKTVTGERVLNDGERHFVSVTIEDGMMTLQNSDHEFEATYVQPVSIQFGDTINVGGLFDVQALTAFGGHFKGCLQDLQLNEKKLEFFPIDTSAMSYKPDRVVEVTAGCTSDDTCSKNPCQNGGICFSSWDDFTCNCPPNTSGQHCEEVSWCDLNPCPPQAICKALNQGYECISNVTFQENTTLVYQGNGLISRHLTSIVFNIRTRKRNAIVLHAESGSEFVTVSLQDGFLVLELLSGPTTSSSLSPVTLHSPRVVADGEWHVIELLMATPGSNSSHWIMVPLDEKDEPTKSDSMTGNLDFLRESVYIMLGGLGPDSGSNLIGCLSNVEIGGIVLPYYGQTEVRFPRTQEEMFNKISEEPVQTGCFGEVVCEPNPCLHGGICDDHFNLFHCFCLPGWGGDHCELNTNTCASNPCRHGYCSVQDLTYNCTCEDGYTGTNCEMKVDACAGHRCANGATCLRGFNMYSCLCTDKFTGPLCNIQIEEVPWYVVVRNIPPKLPVSICGDEQQNYTCFNGGNCSDTNMPCDCHPGFSGHWCELELDECRSNPCKNGGYCQNMVNRFQCVCEMTFAGETCEVDLNAESVTSQLLLSISLVCVVLLLVVFGVTTALVIALNRRATRGTYSPSRQEKEGSRVEMWNIVQPPPMERLI

Target ORF information:

RefSeq Version	NM_001044943.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio crumbs cell polarity complex component 1 (crb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044943.1

ORF Insert Sequence:

ATGGATATGG ATCTAGCGGT TTGGGGTTCA GTGTGGCTCA GATTGGTGGC TTGTGTCTTG 
TTTGTTCTGC TGCAATGGAC AGAAAGTGTA ACACTACTCA AGACCTCAAC ACTCTGCCTG 
GACAACCCTT GCCAACACCA GTCAGAGTGC AGGGAAGCCC TGTCTGATTT CCTGTGCCAA 
TGTCAGACGA CTGTGCCAGT CTTTCCCAGC ACGCGTTGTG ATTCTTCCAG TACGCTCTGC 
CAACTGTCCA TCTGCCAGGG CAATGCGACA TGTCAACCCA CCGGCGCCCA CCCAGGGGAA 
CTAGTTTGCC AGTGTGACTC CGGCTTGTTG GGGCAGGACT GTCTTTCCAG TGCCCAGCTC 
TGCGCTCAGG GGCATTGTGG GGACAGTGCC CACTGTCTGG CAGTGCGAGA CCAGAGTCCA 
GGGTACGCAT GCATCTGTCA GGAGGGTTAC ACTGGGAGAT CTTGCGAGAA AGAGGTGGAC 
CACTGCTCTC CTAACCCCTG CCGAAACCGC GCCATCTGCC GCAGCAGAAG GAATGGGCCC 
ACTTGCTTCT GCGTGCCAGG ATTCCAGGGC CAGCTCTGCG AGATTGAGGT GAATGAATGT 
GTGTCGAGGC CCTGTAGGAA CGGAGCCACC TGTGTGGACA AGATCGGGCA CTACATCTGT 
CTCTGCAGGC CTGGATACAT GGGTAGCAGC TGTGAGCTGG AGATTGATGA GTGTCAGTCA 
CAGCCCTGTC TCCACGGCGC CAGTTGCCAT GACCACATCA ATGGCTTCAC GTGCACATGC 
CTGGCGGGTT TCCAGGGCGA GAGCTGTGAA ATCAACATCG ACGAGTGCCG TGACCAGCCA 
TGCCAAAACG GGGCTCTGTG TGTGGATGAA ATCAATAGCT ACCGCTGTGA CTGCTCACAG 
ACTGCTAACT TCACTGGTGT TGACTGTGAG ATCCCGCCTC CGCCCTGCTG GTCTCAACCC 
TGCCTCAACA GCGCCCTCTG TGAGGATCAA CAGGAGAACT ACACCTGCAA CTGCTGGCCA 
GGGTTTGAGG GGCGTAACTG TGAGGTAGAC GTCAGTGAAT GTGAAAGCAG TCCCTGTGTG 
AATGAAGGCA TTTGCATGGA GCTTTCTTGG AAAACACTTT ACGGTACTGA GCCCCTGTTT 
ACAGCTAGAT ACAATCCCAG ACTTGCTTCC GGGTTCATCT GCAAGTGTCC GCCAGGATTC 
TCAGGTGCCT TGTGCGAGCA AAACACAACT GCCTGTACCA CCAGCCCATG TCATAATGGA 
GCCACATGTG AAGACTTCCT AGGCAGCTAC AAGTGCATCT GCCCTTCTGA AAGCGAAGAT 
GGAGTTCTCT ACGGTGGTCG AAACTGTAGC GAGCCTCTTA CAGGTTGCGA AGGTCACGAG 
TGCCAGAACG GAGCGTCCTG CATCCCATTC CTCAGCGAAG GTGTACATGG ATACAGTTGC 
ATCTGTCAGC CTGGCTACAC CGGCTCTTAC TGCCAAACGC TCACTGTGTT CTCCTTTGAG 
ACAAGCAGAG CATTCTTGCA CCTGCAGACC CCGCTGCTGG GTGCAGAAAC ATACTTCAAT 
ATCACGCTAA GCTTTCGTAC GGTCCTGGAG AACACAGTGC TCTTCCAAAG GGGCAGCGGA 
GGGGTCACAC TCAGCCTGGA GATACAGGAG ACACACCTGA TTCTTGATCT TAAGACTGAC 
CCTCAGCCAA ACGCAACCAG CTGGACTCTG ATGTTGCCTC AGGTTGTATC TGATGGTGAA 
TGGCACACAG TAGAAGCCGT ACTAGGAGAG GGTACCCTGC TGCTGCAGCT GCTGGAGCCA 
TGCCAGGGTG GACAAAACTG TGGTACAACA GCCCAAGTAA AGATTGGCGC CCTTGAGCTG 
GAGTCAGCCC TACTAAGCAC CTTTGTTGGA GGATTGGATG AGGGAGGGAG CTCCAGATCA 
TTCATCGGCT GCATGAGGGA CTTGCTCGTG GACTCGCAGC TGATGATACC GGAGGACTGG 
TTGAGCAGCT CAGCTGTAAA TGTTGTGCAG GGCTGTAGCC ATCATGACCG ATGCCTTTCT 
GGTCCATGCG AGAACCATGG AGAATGTGTT AACCTTTGGC AAGGTTACCA GTGCAAATGT 
CTGCGCCCAT ATGTGGGTCA AAACTGTGAT GAGGAGTATA TTACCACCAG ATTTGGCCAG 
GAAGACTCGA CAAGCTATGC TGTCTTCACC ATCAATGATG AACCTGACTC GGAAACACTT 
CATCTGTCCA TGTTCTTGCG TACTCGAAAG GACTCTGGTC TTTTGGTGCT GCTGGCCAAC 
AGCACCTCTG ACTACTTACA GATGTGGCTG GAAAAGGGAA AACTGACAGT TCAAGTCAAC 
AACTTGAAGA CTGTGACTGG TGAAAGGGTG CTTAATGATG GTGAGAGACA TTTTGTCAGT 
GTCACCATCG AGGATGGCAT GATGACTCTG CAGAACTCGG ATCATGAATT CGAAGCAACA 
TATGTTCAAC CTGTTTCCAT TCAGTTTGGG GATACGATCA ATGTCGGTGG CCTGTTTGAT 
GTACAAGCCT TAACTGCATT TGGTGGACAT TTTAAAGGGT GTCTACAAGA CTTGCAGCTT 
AATGAGAAGA AGCTTGAGTT TTTCCCAATT GATACTTCTG CGATGTCGTA CAAACCCGAT 
CGCGTGGTGG AAGTTACTGC AGGCTGCACC AGTGATGACA CTTGCTCTAA AAATCCTTGC 
CAAAATGGTG GCATCTGCTT TTCTTCATGG GATGATTTTA CTTGCAACTG CCCTCCTAAC 
ACATCAGGAC AACACTGTGA GGAGGTGAGC TGGTGTGACC TCAACCCGTG CCCACCACAA 
GCAATATGCA AGGCCCTAAA CCAGGGCTAT GAATGCATTT CAAACGTGAC TTTCCAGGAG 
AACACCACAC TTGTATACCA AGGCAATGGG CTGATATCCC GCCATCTGAC CAGTATTGTC 
TTTAATATCC GTACACGTAA GCGCAATGCC ATAGTGTTGC ATGCAGAGAG TGGATCAGAG 
TTTGTAACTG TATCTCTTCA AGATGGATTT CTGGTTCTTG AGCTTCTAAG TGGACCTACT 
ACTTCATCAT CATTGTCACC AGTAACCCTG CACAGCCCAA GGGTAGTGGC TGATGGGGAA 
TGGCATGTTA TAGAGCTGTT AATGGCCACT CCAGGGTCTA ACAGCTCCCA CTGGATTATG 
GTCCCCCTAG ATGAAAAAGA TGAGCCAACT AAATCAGACT CTATGACCGG AAATCTAGAC 
TTCCTCAGAG AGAGTGTGTA CATCATGCTA GGAGGACTAG GCCCTGATTC TGGCTCCAAT 
CTAATTGGTT GCTTGAGTAA TGTTGAGATT GGTGGAATCG TGCTTCCGTA TTATGGACAG 
ACTGAGGTGA GATTCCCACG CACACAGGAA GAAATGTTCA ATAAGATATC AGAGGAGCCT 
GTTCAAACCG GCTGTTTCGG GGAGGTGGTG TGTGAGCCCA ATCCCTGCCT GCATGGAGGC 
ATCTGTGACG ATCACTTCAA CCTTTTTCAC TGCTTCTGTC TTCCTGGCTG GGGCGGTGAT 
CACTGTGAGC TGAATACAAA CACCTGCGCC TCCAACCCCT GTCGACATGG ATATTGTAGT 
GTGCAGGACC TCACATACAA CTGCACATGT GAGGACGGAT ACACTGGCAC CAACTGTGAG 
ATGAAGGTGG ATGCTTGTGC TGGGCACAGG TGTGCTAATG GAGCGACCTG CTTGCGTGGC 
TTCAACATGT ATTCCTGCCT CTGTACCGAC AAGTTTACTG GGCCACTTTG CAACATTCAG 
ATTGAAGAGG TTCCGTGGTA CGTAGTTGTC AGGAACATAC CGCCAAAGCT CCCGGTGTCC 
ATCTGTGGCG ATGAACAGCA GAACTACACA TGTTTCAATG GGGGGAACTG TTCTGACACT 
AACATGCCGT GCGACTGCCA CCCTGGGTTT TCGGGTCACT GGTGTGAACT AGAACTTGAT 
GAATGTAGAT CCAACCCTTG CAAGAATGGC GGCTACTGCC AGAACATGGT CAACAGGTTC 
CAATGCGTCT GTGAAATGAC TTTTGCCGGC GAGACCTGCG AAGTGGACCT GAACGCAGAG 
AGTGTGACGT CTCAGCTGTT GCTCTCCATC AGCCTGGTGT GTGTGGTTCT GCTCTTGGTT 
GTTTTTGGTG TGACCACAGC TCTGGTCATT GCACTGAACC GAAGGGCCAC ACGGGGCACT 
TACAGCCCCA GTCGGCAGGA GAAGGAAGGC TCAAGGGTGG AAATGTGGAA CATTGTACAA 
CCTCCACCAA TGGAGAGACT CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crb1 ( XM_005161738.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161795.1 Danio rerio crumbs family member 1, photoreceptor morphogenesis associated (crb1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27000
Clone ID Related Accession (Same CDS sequence)	XM_005161738.3 , XM_005161738.4
Accession Version	XM_005161738.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	protein crumbs homolog 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161738.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTTG TAGTTGGGAT TTGGAGTGTA CTGTTACTCA TTTCAGGTGC CTTGTGCGAG 
CAAAACACAA CTGCCTGTAC CACCAGCCCA TGTCATAATG GAGCCACATG TGAAGACTTC 
CTAGGCAGCT ACAAGTGCAT CTGCCCTTCT GAAAGCCAAG ATGGAGTCCT CTACGGTGGT 
CGAAACTGTA GCGAGCCTCT TACAGGTTGC GAAGGTCACG AGTGCCAGAA CGGAGCGTCC 
TGCATCCCAT TCCTCAGCGA AGGTGTACAT GGATACAGTT GCATCTGTCA GCCTGGCTAC 
ACCGGCTCTT ACTGCCAAAC GCTCACTGTG TTCTCCTTTG AGACAAGCAG AGCATTCTTG 
CACCTGCAGA CCCCGCTGCT GGGTGCAGAA ACATATTTCA ATATCACGCT AAGCTTTCGT 
ACAGTCCTGG AGAACACAGT GCTCTTCCAA AGGGGCAGCG GAGGGGTCAC ACTCAGCCTG 
GAGATACAGG AGACACACCT GATTCTTGAT CTTAAGACTG ACCCTCAGCC AAACGCAACC 
AGCTGGACTC TGATGTTGCC TCAGGTTGTA TCTGATGGTG AATGGCACAC AGTAGAAGCC 
GTACTTGGAG AGGGTACCCT GCTGCTGCAG CTGCTGGAGC CATGCCAGGG TGGACAAAAC 
TGTGGTACAA CAGCCCAAGT AAAGATCGGC GCCCTTGAGC TGGAGTCAGC CCTACTAAGC 
ACCTTTGTTG GAGGATTGGA TGAGGGAGGG AGCTCCAGAT CATTCATCGG CTGCATGAGG 
GACTTGCTCG TGGACTCGCA GCTGATGATA CCGGAGGACT GGTTGAGCAG CTCAGCTGTA 
AATGTTGTGC AGGGCTGTAG CCATCATGAC CGATGCCTTT CTGGTCCATG CGAGAACCAT 
GGAGAATGTG TTAACCTTTG GCAAGGTTAC CAGTGCAAAT GTCTGCGCCC ATATGTGGGT 
CAAAACTGTG ATGAGGAGTA TATTACCGCC AGATTTGCCC AGCAAGACTC GACAAGCTAT 
GCTGTCTTCA CCATCAATGA CGAACCTGAC TCGGAAACAC TTCATCTGTC CATGTTCTTG 
CGTACTCGAA AAGACTCTGG TCTTTTGGTG CTGCTGGCCA ACAGCACCTC TGACTACTTG 
CAGATGTGGC TGGAAAAGGG AAAACTGACA GTTCAAGTCA ACAACTTGAA GACTGTGACT 
GGTGAAAGGG TGCTTAATGA TGGTGAGAGA CATTTTGTCA GTGTCACCAT CGAGGATGGC 
ATGATGACTC TGCAGGACTC GGATCATGAA TTCGAAGCAA CATATGTTCA ACCTGTTTCC 
ATTCAGTTTG GGGATACGAT CAATGTCGGT GGCCTGTTTG ATGTACAAGC CTTAACTGCG 
TTTGGTGGAC ATTTTAAAGG TTGTCTACAA GACTTGCAGC TTAATGAGAA GAAGCTTGAG 
TTTTTCCCAA TTGATACTTC TGCGATGTCG TACAAACCCG ATCGCGTTGT GAAAGTTACT 
GCAGGCTGCA CCAGTGATGA CACTTGCTCA AAAAATCCTT GCCAAAATGG TGGCATCTGC 
TTTTCTTCAT GGGATGATTT TACTTGCAAC TGCCCTCCTA ACACATCAGG ACAACACTGT 
GAGGAGGTGA GCTGGTGTGA CCTCAACCCG TGCCCACCAC AAGCAATATG CCGGGCCCTA 
AACCAGGGCT ATGAATGCAT TTCAAACGTG ACTTTCCAGG AGAACACCAC ACTTGTATAC 
CAAGGCAATG GGCTGATATC CCGCCATCTG ACCAGTATTG TCTTTAATAT CCGTACACGT 
AAGCGCAATG CCATAGTGTT GCATGCAGAG AGTGGATCAG AGTTTGTAAC TGTATCTCTT 
CAAGATGGAT TTCTGGTTCT TGAGCTTCTA AGTGGACCTA CTACTTCATC CTCATTGTCA 
CCAGTAACCC TGCACAGCCC AAGGGTAGTC GCTGATGGGG AATGGCATGT TATAGAGCTG 
TTAATGGCCA CTCCAGGGTC TAACAGCTCC CACTGGATTA TGGTCCCCCT AGATGAAAAA 
GATGAGCCTA CTAAATCAGA CTCTATGACC GGAAATCTAG ACTTCCTCAG AGAGAGCGTG 
TACATCATGC TAGGAGGACT AGGCCCTGAT TCTGGCTCCA ATCTCATTGG TTGCTTGAGT 
AATGTTGAGA TTGGTGGAAT CGTGCTTCCG TATTATGGAC AGACTGAGGT GAGATTTCCA 
CGCACACAGG AAGAAATGTT CAATAAGATA TCCGAGGAGC CTGTTCAAAC CGGCTGTTTC 
GGGGAGGTGG TGTGTGAGCC CAATCCCTGC CTGCATGGAG GCATCTGTGA CGATCACTTC 
AACCTTTTTC ACTGCTTCTG TCTTCCTGGC TGGGGCGGTG ATCACTGTGA GCTGAATACA 
AACACCTGCG CCTCCAACCC CTGTCGACAT GGATATTGTA GTGTGCAGGA CCTCACATAC 
AACTGCACAT GTGAGGACGG ATACACTGGC ACCAACTGTG AGATGAAGGT GGATGCTTGT 
GCTGGGCACA GGTGTGCTAA TGGAGCGACC TGCTTGCGTG GCTTCAACAT GTATTCCTGC 
CTCTGTACCG ACAAGTTTAC TGGGCCACTT TGCAACATTC AGATTGAAGA GGTTCCGTGG 
TACGTAGTTG TCAGGAACAT ACCGCCAAAG CTCCCGGTGT CCATCTGTGG CGATGAACAG 
CAGAACTACA CATGTTTCAA TGGGGGGAAC TGTTCTGACA CCAACATGCC GTGCGACTGC 
CACCCTGGGT TTTCGGGTCA CTGGTGTGAA CTAGAACTTG ATGAATGTAG ATCCAATCCT 
TGCAAGAATG GCGGCTACTG CCAGAACATG GTCAACAGGT TCCAATGCGT CTGTGAAATG 
ACTTTTGCCG GCGAGACCTG CGAAGTGGAC CTGAACGCAG AGAGTGTGAC GTCTCAGCTG 
TTGCTCTCCA TCAGCCTGGT GTGTGTGGTT CTGCTCTTGG TTGTTTTTGG TGTGACCACA 
GCTCTGGTCA TTGCACTGAA CCGAAGGGCC ACACGGGGCA CTTACAGCCC CAGTCGGCAG 
GAGAAGGAAG GCTCAAGGGT GGAAATGTGG AACATTGTAC AACCTCCACC AATGGAGAGA 
CTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161795.1
CDS	166..3294
Translation	MEVVVGIWSVLLLISGALCEQNTTACTTSPCHNGATCEDFLGSYKCICPSESQDGVLYGGRNCSEPLTGCEGHECQNGASCIPFLSEGVHGYSCICQPGYTGSYCQTLTVFSFETSRAFLHLQTPLLGAETYFNITLSFRTVLENTVLFQRGSGGVTLSLEIQETHLILDLKTDPQPNATSWTLMLPQVVSDGEWHTVEAVLGEGTLLLQLLEPCQGGQNCGTTAQVKIGALELESALLSTFVGGLDEGGSSRSFIGCMRDLLVDSQLMIPEDWLSSSAVNVVQGCSHHDRCLSGPCENHGECVNLWQGYQCKCLRPYVGQNCDEEYITARFAQQDSTSYAVFTINDEPDSETLHLSMFLRTRKDSGLLVLLANSTSDYLQMWLEKGKLTVQVNNLKTVTGERVLNDGERHFVSVTIEDGMMTLQDSDHEFEATYVQPVSIQFGDTINVGGLFDVQALTAFGGHFKGCLQDLQLNEKKLEFFPIDTSAMSYKPDRVVKVTAGCTSDDTCSKNPCQNGGICFSSWDDFTCNCPPNTSGQHCEEVSWCDLNPCPPQAICRALNQGYECISNVTFQENTTLVYQGNGLISRHLTSIVFNIRTRKRNAIVLHAESGSEFVTVSLQDGFLVLELLSGPTTSSSLSPVTLHSPRVVADGEWHVIELLMATPGSNSSHWIMVPLDEKDEPTKSDSMTGNLDFLRESVYIMLGGLGPDSGSNLIGCLSNVEIGGIVLPYYGQTEVRFPRTQEEMFNKISEEPVQTGCFGEVVCEPNPCLHGGICDDHFNLFHCFCLPGWGGDHCELNTNTCASNPCRHGYCSVQDLTYNCTCEDGYTGTNCEMKVDACAGHRCANGATCLRGFNMYSCLCTDKFTGPLCNIQIEEVPWYVVVRNIPPKLPVSICGDEQQNYTCFNGGNCSDTNMPCDCHPGFSGHWCELELDECRSNPCKNGGYCQNMVNRFQCVCEMTFAGETCEVDLNAESVTSQLLLSISLVCVVLLLVVFGVTTALVIALNRRATRGTYSPSRQEKEGSRVEMWNIVQPPPMERLI

Target ORF information:

RefSeq Version	XM_005161738.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio crumbs family member 1, photoreceptor morphogenesis associated (crb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161738.3

ORF Insert Sequence:

ATGGAAGTTG TAGTTGGGAT TTGGAGTGTA CTGTTACTCA TTTCAGGTGC CTTGTGCGAG 
CAAAACACAA CTGCCTGTAC CACCAGCCCA TGTCATAATG GAGCCACATG TGAAGACTTC 
CTAGGCAGCT ACAAGTGCAT CTGCCCTTCT GAAAGCCAAG ATGGAGTCCT CTACGGTGGT 
CGAAACTGTA GCGAGCCTCT TACAGGTTGC GAAGGTCACG AGTGCCAGAA CGGAGCGTCC 
TGCATCCCAT TCCTCAGCGA AGGTGTACAT GGATACAGTT GCATCTGTCA GCCTGGCTAC 
ACCGGCTCTT ACTGCCAAAC GCTCACTGTG TTCTCCTTTG AGACAAGCAG AGCATTCTTG 
CACCTGCAGA CCCCGCTGCT GGGTGCAGAA ACATATTTCA ATATCACGCT AAGCTTTCGT 
ACAGTCCTGG AGAACACAGT GCTCTTCCAA AGGGGCAGCG GAGGGGTCAC ACTCAGCCTG 
GAGATACAGG AGACACACCT GATTCTTGAT CTTAAGACTG ACCCTCAGCC AAACGCAACC 
AGCTGGACTC TGATGTTGCC TCAGGTTGTA TCTGATGGTG AATGGCACAC AGTAGAAGCC 
GTACTTGGAG AGGGTACCCT GCTGCTGCAG CTGCTGGAGC CATGCCAGGG TGGACAAAAC 
TGTGGTACAA CAGCCCAAGT AAAGATCGGC GCCCTTGAGC TGGAGTCAGC CCTACTAAGC 
ACCTTTGTTG GAGGATTGGA TGAGGGAGGG AGCTCCAGAT CATTCATCGG CTGCATGAGG 
GACTTGCTCG TGGACTCGCA GCTGATGATA CCGGAGGACT GGTTGAGCAG CTCAGCTGTA 
AATGTTGTGC AGGGCTGTAG CCATCATGAC CGATGCCTTT CTGGTCCATG CGAGAACCAT 
GGAGAATGTG TTAACCTTTG GCAAGGTTAC CAGTGCAAAT GTCTGCGCCC ATATGTGGGT 
CAAAACTGTG ATGAGGAGTA TATTACCGCC AGATTTGCCC AGCAAGACTC GACAAGCTAT 
GCTGTCTTCA CCATCAATGA CGAACCTGAC TCGGAAACAC TTCATCTGTC CATGTTCTTG 
CGTACTCGAA AAGACTCTGG TCTTTTGGTG CTGCTGGCCA ACAGCACCTC TGACTACTTG 
CAGATGTGGC TGGAAAAGGG AAAACTGACA GTTCAAGTCA ACAACTTGAA GACTGTGACT 
GGTGAAAGGG TGCTTAATGA TGGTGAGAGA CATTTTGTCA GTGTCACCAT CGAGGATGGC 
ATGATGACTC TGCAGGACTC GGATCATGAA TTCGAAGCAA CATATGTTCA ACCTGTTTCC 
ATTCAGTTTG GGGATACGAT CAATGTCGGT GGCCTGTTTG ATGTACAAGC CTTAACTGCG 
TTTGGTGGAC ATTTTAAAGG TTGTCTACAA GACTTGCAGC TTAATGAGAA GAAGCTTGAG 
TTTTTCCCAA TTGATACTTC TGCGATGTCG TACAAACCCG ATCGCGTTGT GAAAGTTACT 
GCAGGCTGCA CCAGTGATGA CACTTGCTCA AAAAATCCTT GCCAAAATGG TGGCATCTGC 
TTTTCTTCAT GGGATGATTT TACTTGCAAC TGCCCTCCTA ACACATCAGG ACAACACTGT 
GAGGAGGTGA GCTGGTGTGA CCTCAACCCG TGCCCACCAC AAGCAATATG CCGGGCCCTA 
AACCAGGGCT ATGAATGCAT TTCAAACGTG ACTTTCCAGG AGAACACCAC ACTTGTATAC 
CAAGGCAATG GGCTGATATC CCGCCATCTG ACCAGTATTG TCTTTAATAT CCGTACACGT 
AAGCGCAATG CCATAGTGTT GCATGCAGAG AGTGGATCAG AGTTTGTAAC TGTATCTCTT 
CAAGATGGAT TTCTGGTTCT TGAGCTTCTA AGTGGACCTA CTACTTCATC CTCATTGTCA 
CCAGTAACCC TGCACAGCCC AAGGGTAGTC GCTGATGGGG AATGGCATGT TATAGAGCTG 
TTAATGGCCA CTCCAGGGTC TAACAGCTCC CACTGGATTA TGGTCCCCCT AGATGAAAAA 
GATGAGCCTA CTAAATCAGA CTCTATGACC GGAAATCTAG ACTTCCTCAG AGAGAGCGTG 
TACATCATGC TAGGAGGACT AGGCCCTGAT TCTGGCTCCA ATCTCATTGG TTGCTTGAGT 
AATGTTGAGA TTGGTGGAAT CGTGCTTCCG TATTATGGAC AGACTGAGGT GAGATTTCCA 
CGCACACAGG AAGAAATGTT CAATAAGATA TCCGAGGAGC CTGTTCAAAC CGGCTGTTTC 
GGGGAGGTGG TGTGTGAGCC CAATCCCTGC CTGCATGGAG GCATCTGTGA CGATCACTTC 
AACCTTTTTC ACTGCTTCTG TCTTCCTGGC TGGGGCGGTG ATCACTGTGA GCTGAATACA 
AACACCTGCG CCTCCAACCC CTGTCGACAT GGATATTGTA GTGTGCAGGA CCTCACATAC 
AACTGCACAT GTGAGGACGG ATACACTGGC ACCAACTGTG AGATGAAGGT GGATGCTTGT 
GCTGGGCACA GGTGTGCTAA TGGAGCGACC TGCTTGCGTG GCTTCAACAT GTATTCCTGC 
CTCTGTACCG ACAAGTTTAC TGGGCCACTT TGCAACATTC AGATTGAAGA GGTTCCGTGG 
TACGTAGTTG TCAGGAACAT ACCGCCAAAG CTCCCGGTGT CCATCTGTGG CGATGAACAG 
CAGAACTACA CATGTTTCAA TGGGGGGAAC TGTTCTGACA CCAACATGCC GTGCGACTGC 
CACCCTGGGT TTTCGGGTCA CTGGTGTGAA CTAGAACTTG ATGAATGTAG ATCCAATCCT 
TGCAAGAATG GCGGCTACTG CCAGAACATG GTCAACAGGT TCCAATGCGT CTGTGAAATG 
ACTTTTGCCG GCGAGACCTG CGAAGTGGAC CTGAACGCAG AGAGTGTGAC GTCTCAGCTG 
TTGCTCTCCA TCAGCCTGGT GTGTGTGGTT CTGCTCTTGG TTGTTTTTGG TGTGACCACA 
GCTCTGGTCA TTGCACTGAA CCGAAGGGCC ACACGGGGCA CTTACAGCCC CAGTCGGCAG 
GAGAAGGAAG GCTCAAGGGT GGAAATGTGG AACATTGTAC AACCTCCACC AATGGAGAGA 
CTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crb1 ( XM_005161738.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161795.1 Danio rerio crumbs family member 1, photoreceptor morphogenesis associated (crb1), transcript variant X1, mRNA.

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CloneID	ODa27000
Clone ID Related Accession (Same CDS sequence)	XM_005161738.3 , XM_005161738.4
Accession Version	XM_005161738.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	protein crumbs homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161738.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTTG TAGTTGGGAT TTGGAGTGTA CTGTTACTCA TTTCAGGTGC CTTGTGCGAG 
CAAAACACAA CTGCCTGTAC CACCAGCCCA TGTCATAATG GAGCCACATG TGAAGACTTC 
CTAGGCAGCT ACAAGTGCAT CTGCCCTTCT GAAAGCCAAG ATGGAGTCCT CTACGGTGGT 
CGAAACTGTA GCGAGCCTCT TACAGGTTGC GAAGGTCACG AGTGCCAGAA CGGAGCGTCC 
TGCATCCCAT TCCTCAGCGA AGGTGTACAT GGATACAGTT GCATCTGTCA GCCTGGCTAC 
ACCGGCTCTT ACTGCCAAAC GCTCACTGTG TTCTCCTTTG AGACAAGCAG AGCATTCTTG 
CACCTGCAGA CCCCGCTGCT GGGTGCAGAA ACATATTTCA ATATCACGCT AAGCTTTCGT 
ACAGTCCTGG AGAACACAGT GCTCTTCCAA AGGGGCAGCG GAGGGGTCAC ACTCAGCCTG 
GAGATACAGG AGACACACCT GATTCTTGAT CTTAAGACTG ACCCTCAGCC AAACGCAACC 
AGCTGGACTC TGATGTTGCC TCAGGTTGTA TCTGATGGTG AATGGCACAC AGTAGAAGCC 
GTACTTGGAG AGGGTACCCT GCTGCTGCAG CTGCTGGAGC CATGCCAGGG TGGACAAAAC 
TGTGGTACAA CAGCCCAAGT AAAGATCGGC GCCCTTGAGC TGGAGTCAGC CCTACTAAGC 
ACCTTTGTTG GAGGATTGGA TGAGGGAGGG AGCTCCAGAT CATTCATCGG CTGCATGAGG 
GACTTGCTCG TGGACTCGCA GCTGATGATA CCGGAGGACT GGTTGAGCAG CTCAGCTGTA 
AATGTTGTGC AGGGCTGTAG CCATCATGAC CGATGCCTTT CTGGTCCATG CGAGAACCAT 
GGAGAATGTG TTAACCTTTG GCAAGGTTAC CAGTGCAAAT GTCTGCGCCC ATATGTGGGT 
CAAAACTGTG ATGAGGAGTA TATTACCGCC AGATTTGCCC AGCAAGACTC GACAAGCTAT 
GCTGTCTTCA CCATCAATGA CGAACCTGAC TCGGAAACAC TTCATCTGTC CATGTTCTTG 
CGTACTCGAA AAGACTCTGG TCTTTTGGTG CTGCTGGCCA ACAGCACCTC TGACTACTTG 
CAGATGTGGC TGGAAAAGGG AAAACTGACA GTTCAAGTCA ACAACTTGAA GACTGTGACT 
GGTGAAAGGG TGCTTAATGA TGGTGAGAGA CATTTTGTCA GTGTCACCAT CGAGGATGGC 
ATGATGACTC TGCAGGACTC GGATCATGAA TTCGAAGCAA CATATGTTCA ACCTGTTTCC 
ATTCAGTTTG GGGATACGAT CAATGTCGGT GGCCTGTTTG ATGTACAAGC CTTAACTGCG 
TTTGGTGGAC ATTTTAAAGG TTGTCTACAA GACTTGCAGC TTAATGAGAA GAAGCTTGAG 
TTTTTCCCAA TTGATACTTC TGCGATGTCG TACAAACCCG ATCGCGTTGT GAAAGTTACT 
GCAGGCTGCA CCAGTGATGA CACTTGCTCA AAAAATCCTT GCCAAAATGG TGGCATCTGC 
TTTTCTTCAT GGGATGATTT TACTTGCAAC TGCCCTCCTA ACACATCAGG ACAACACTGT 
GAGGAGGTGA GCTGGTGTGA CCTCAACCCG TGCCCACCAC AAGCAATATG CCGGGCCCTA 
AACCAGGGCT ATGAATGCAT TTCAAACGTG ACTTTCCAGG AGAACACCAC ACTTGTATAC 
CAAGGCAATG GGCTGATATC CCGCCATCTG ACCAGTATTG TCTTTAATAT CCGTACACGT 
AAGCGCAATG CCATAGTGTT GCATGCAGAG AGTGGATCAG AGTTTGTAAC TGTATCTCTT 
CAAGATGGAT TTCTGGTTCT TGAGCTTCTA AGTGGACCTA CTACTTCATC CTCATTGTCA 
CCAGTAACCC TGCACAGCCC AAGGGTAGTC GCTGATGGGG AATGGCATGT TATAGAGCTG 
TTAATGGCCA CTCCAGGGTC TAACAGCTCC CACTGGATTA TGGTCCCCCT AGATGAAAAA 
GATGAGCCTA CTAAATCAGA CTCTATGACC GGAAATCTAG ACTTCCTCAG AGAGAGCGTG 
TACATCATGC TAGGAGGACT AGGCCCTGAT TCTGGCTCCA ATCTCATTGG TTGCTTGAGT 
AATGTTGAGA TTGGTGGAAT CGTGCTTCCG TATTATGGAC AGACTGAGGT GAGATTTCCA 
CGCACACAGG AAGAAATGTT CAATAAGATA TCCGAGGAGC CTGTTCAAAC CGGCTGTTTC 
GGGGAGGTGG TGTGTGAGCC CAATCCCTGC CTGCATGGAG GCATCTGTGA CGATCACTTC 
AACCTTTTTC ACTGCTTCTG TCTTCCTGGC TGGGGCGGTG ATCACTGTGA GCTGAATACA 
AACACCTGCG CCTCCAACCC CTGTCGACAT GGATATTGTA GTGTGCAGGA CCTCACATAC 
AACTGCACAT GTGAGGACGG ATACACTGGC ACCAACTGTG AGATGAAGGT GGATGCTTGT 
GCTGGGCACA GGTGTGCTAA TGGAGCGACC TGCTTGCGTG GCTTCAACAT GTATTCCTGC 
CTCTGTACCG ACAAGTTTAC TGGGCCACTT TGCAACATTC AGATTGAAGA GGTTCCGTGG 
TACGTAGTTG TCAGGAACAT ACCGCCAAAG CTCCCGGTGT CCATCTGTGG CGATGAACAG 
CAGAACTACA CATGTTTCAA TGGGGGGAAC TGTTCTGACA CCAACATGCC GTGCGACTGC 
CACCCTGGGT TTTCGGGTCA CTGGTGTGAA CTAGAACTTG ATGAATGTAG ATCCAATCCT 
TGCAAGAATG GCGGCTACTG CCAGAACATG GTCAACAGGT TCCAATGCGT CTGTGAAATG 
ACTTTTGCCG GCGAGACCTG CGAAGTGGAC CTGAACGCAG AGAGTGTGAC GTCTCAGCTG 
TTGCTCTCCA TCAGCCTGGT GTGTGTGGTT CTGCTCTTGG TTGTTTTTGG TGTGACCACA 
GCTCTGGTCA TTGCACTGAA CCGAAGGGCC ACACGGGGCA CTTACAGCCC CAGTCGGCAG 
GAGAAGGAAG GCTCAAGGGT GGAAATGTGG AACATTGTAC AACCTCCACC AATGGAGAGA 
CTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161795.1
CDS	181..3309
Translation	MEVVVGIWSVLLLISGALCEQNTTACTTSPCHNGATCEDFLGSYKCICPSESQDGVLYGGRNCSEPLTGCEGHECQNGASCIPFLSEGVHGYSCICQPGYTGSYCQTLTVFSFETSRAFLHLQTPLLGAETYFNITLSFRTVLENTVLFQRGSGGVTLSLEIQETHLILDLKTDPQPNATSWTLMLPQVVSDGEWHTVEAVLGEGTLLLQLLEPCQGGQNCGTTAQVKIGALELESALLSTFVGGLDEGGSSRSFIGCMRDLLVDSQLMIPEDWLSSSAVNVVQGCSHHDRCLSGPCENHGECVNLWQGYQCKCLRPYVGQNCDEEYITARFAQQDSTSYAVFTINDEPDSETLHLSMFLRTRKDSGLLVLLANSTSDYLQMWLEKGKLTVQVNNLKTVTGERVLNDGERHFVSVTIEDGMMTLQDSDHEFEATYVQPVSIQFGDTINVGGLFDVQALTAFGGHFKGCLQDLQLNEKKLEFFPIDTSAMSYKPDRVVKVTAGCTSDDTCSKNPCQNGGICFSSWDDFTCNCPPNTSGQHCEEVSWCDLNPCPPQAICRALNQGYECISNVTFQENTTLVYQGNGLISRHLTSIVFNIRTRKRNAIVLHAESGSEFVTVSLQDGFLVLELLSGPTTSSSLSPVTLHSPRVVADGEWHVIELLMATPGSNSSHWIMVPLDEKDEPTKSDSMTGNLDFLRESVYIMLGGLGPDSGSNLIGCLSNVEIGGIVLPYYGQTEVRFPRTQEEMFNKISEEPVQTGCFGEVVCEPNPCLHGGICDDHFNLFHCFCLPGWGGDHCELNTNTCASNPCRHGYCSVQDLTYNCTCEDGYTGTNCEMKVDACAGHRCANGATCLRGFNMYSCLCTDKFTGPLCNIQIEEVPWYVVVRNIPPKLPVSICGDEQQNYTCFNGGNCSDTNMPCDCHPGFSGHWCELELDECRSNPCKNGGYCQNMVNRFQCVCEMTFAGETCEVDLNAESVTSQLLLSISLVCVVLLLVVFGVTTALVIALNRRATRGTYSPSRQEKEGSRVEMWNIVQPPPMERLI

Target ORF information:

RefSeq Version	XM_005161738.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio crumbs family member 1, photoreceptor morphogenesis associated (crb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161738.4

ORF Insert Sequence:

ATGGAAGTTG TAGTTGGGAT TTGGAGTGTA CTGTTACTCA TTTCAGGTGC CTTGTGCGAG 
CAAAACACAA CTGCCTGTAC CACCAGCCCA TGTCATAATG GAGCCACATG TGAAGACTTC 
CTAGGCAGCT ACAAGTGCAT CTGCCCTTCT GAAAGCCAAG ATGGAGTCCT CTACGGTGGT 
CGAAACTGTA GCGAGCCTCT TACAGGTTGC GAAGGTCACG AGTGCCAGAA CGGAGCGTCC 
TGCATCCCAT TCCTCAGCGA AGGTGTACAT GGATACAGTT GCATCTGTCA GCCTGGCTAC 
ACCGGCTCTT ACTGCCAAAC GCTCACTGTG TTCTCCTTTG AGACAAGCAG AGCATTCTTG 
CACCTGCAGA CCCCGCTGCT GGGTGCAGAA ACATATTTCA ATATCACGCT AAGCTTTCGT 
ACAGTCCTGG AGAACACAGT GCTCTTCCAA AGGGGCAGCG GAGGGGTCAC ACTCAGCCTG 
GAGATACAGG AGACACACCT GATTCTTGAT CTTAAGACTG ACCCTCAGCC AAACGCAACC 
AGCTGGACTC TGATGTTGCC TCAGGTTGTA TCTGATGGTG AATGGCACAC AGTAGAAGCC 
GTACTTGGAG AGGGTACCCT GCTGCTGCAG CTGCTGGAGC CATGCCAGGG TGGACAAAAC 
TGTGGTACAA CAGCCCAAGT AAAGATCGGC GCCCTTGAGC TGGAGTCAGC CCTACTAAGC 
ACCTTTGTTG GAGGATTGGA TGAGGGAGGG AGCTCCAGAT CATTCATCGG CTGCATGAGG 
GACTTGCTCG TGGACTCGCA GCTGATGATA CCGGAGGACT GGTTGAGCAG CTCAGCTGTA 
AATGTTGTGC AGGGCTGTAG CCATCATGAC CGATGCCTTT CTGGTCCATG CGAGAACCAT 
GGAGAATGTG TTAACCTTTG GCAAGGTTAC CAGTGCAAAT GTCTGCGCCC ATATGTGGGT 
CAAAACTGTG ATGAGGAGTA TATTACCGCC AGATTTGCCC AGCAAGACTC GACAAGCTAT 
GCTGTCTTCA CCATCAATGA CGAACCTGAC TCGGAAACAC TTCATCTGTC CATGTTCTTG 
CGTACTCGAA AAGACTCTGG TCTTTTGGTG CTGCTGGCCA ACAGCACCTC TGACTACTTG 
CAGATGTGGC TGGAAAAGGG AAAACTGACA GTTCAAGTCA ACAACTTGAA GACTGTGACT 
GGTGAAAGGG TGCTTAATGA TGGTGAGAGA CATTTTGTCA GTGTCACCAT CGAGGATGGC 
ATGATGACTC TGCAGGACTC GGATCATGAA TTCGAAGCAA CATATGTTCA ACCTGTTTCC 
ATTCAGTTTG GGGATACGAT CAATGTCGGT GGCCTGTTTG ATGTACAAGC CTTAACTGCG 
TTTGGTGGAC ATTTTAAAGG TTGTCTACAA GACTTGCAGC TTAATGAGAA GAAGCTTGAG 
TTTTTCCCAA TTGATACTTC TGCGATGTCG TACAAACCCG ATCGCGTTGT GAAAGTTACT 
GCAGGCTGCA CCAGTGATGA CACTTGCTCA AAAAATCCTT GCCAAAATGG TGGCATCTGC 
TTTTCTTCAT GGGATGATTT TACTTGCAAC TGCCCTCCTA ACACATCAGG ACAACACTGT 
GAGGAGGTGA GCTGGTGTGA CCTCAACCCG TGCCCACCAC AAGCAATATG CCGGGCCCTA 
AACCAGGGCT ATGAATGCAT TTCAAACGTG ACTTTCCAGG AGAACACCAC ACTTGTATAC 
CAAGGCAATG GGCTGATATC CCGCCATCTG ACCAGTATTG TCTTTAATAT CCGTACACGT 
AAGCGCAATG CCATAGTGTT GCATGCAGAG AGTGGATCAG AGTTTGTAAC TGTATCTCTT 
CAAGATGGAT TTCTGGTTCT TGAGCTTCTA AGTGGACCTA CTACTTCATC CTCATTGTCA 
CCAGTAACCC TGCACAGCCC AAGGGTAGTC GCTGATGGGG AATGGCATGT TATAGAGCTG 
TTAATGGCCA CTCCAGGGTC TAACAGCTCC CACTGGATTA TGGTCCCCCT AGATGAAAAA 
GATGAGCCTA CTAAATCAGA CTCTATGACC GGAAATCTAG ACTTCCTCAG AGAGAGCGTG 
TACATCATGC TAGGAGGACT AGGCCCTGAT TCTGGCTCCA ATCTCATTGG TTGCTTGAGT 
AATGTTGAGA TTGGTGGAAT CGTGCTTCCG TATTATGGAC AGACTGAGGT GAGATTTCCA 
CGCACACAGG AAGAAATGTT CAATAAGATA TCCGAGGAGC CTGTTCAAAC CGGCTGTTTC 
GGGGAGGTGG TGTGTGAGCC CAATCCCTGC CTGCATGGAG GCATCTGTGA CGATCACTTC 
AACCTTTTTC ACTGCTTCTG TCTTCCTGGC TGGGGCGGTG ATCACTGTGA GCTGAATACA 
AACACCTGCG CCTCCAACCC CTGTCGACAT GGATATTGTA GTGTGCAGGA CCTCACATAC 
AACTGCACAT GTGAGGACGG ATACACTGGC ACCAACTGTG AGATGAAGGT GGATGCTTGT 
GCTGGGCACA GGTGTGCTAA TGGAGCGACC TGCTTGCGTG GCTTCAACAT GTATTCCTGC 
CTCTGTACCG ACAAGTTTAC TGGGCCACTT TGCAACATTC AGATTGAAGA GGTTCCGTGG 
TACGTAGTTG TCAGGAACAT ACCGCCAAAG CTCCCGGTGT CCATCTGTGG CGATGAACAG 
CAGAACTACA CATGTTTCAA TGGGGGGAAC TGTTCTGACA CCAACATGCC GTGCGACTGC 
CACCCTGGGT TTTCGGGTCA CTGGTGTGAA CTAGAACTTG ATGAATGTAG ATCCAATCCT 
TGCAAGAATG GCGGCTACTG CCAGAACATG GTCAACAGGT TCCAATGCGT CTGTGAAATG 
ACTTTTGCCG GCGAGACCTG CGAAGTGGAC CTGAACGCAG AGAGTGTGAC GTCTCAGCTG 
TTGCTCTCCA TCAGCCTGGT GTGTGTGGTT CTGCTCTTGG TTGTTTTTGG TGTGACCACA 
GCTCTGGTCA TTGCACTGAA CCGAAGGGCC ACACGGGGCA CTTACAGCCC CAGTCGGCAG 
GAGAAGGAAG GCTCAAGGGT GGAAATGTGG AACATTGTAC AACCTCCACC AATGGAGAGA 
CTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Notch-mediated inhibition of neurogenesis is required for zebrafish spinal cord morphogenesis.
Scientific reports9(1)9958(2019 Jul)
Sharma P,Saraswathy VM,Xiang L,Fürthauer M

Regulation of the apical extension morphogenesis tunes the mechanosensory response of microvilliated neurons.
PLoS biology17(4)e3000235(2019 04)
Desban L,Prendergast A,Roussel J,Rosello M,Geny D,Wyart C,Bardet PL

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Spatial-temporal expressions of Crumbs and Nagie oko and their interdependence in zebrafish central nervous system during early development.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience31(8)770-82(2013 Dec)
Zou J,Wen Y,Yang X,Wei X

Crb apical polarity proteins maintain zebrafish retinal cone mosaics via intercellular binding of their extracellular domains.
Developmental cell22(6)1261-74(2012 Jun)
Zou J,Wang X,Wei X

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crb1 cDNA ORF clone, Danio rerio(zebrafish)

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