asap2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	asap2b
Entrez Gene ID	560581
Full Name	ArfGAP with SH3 domain, ankyrin repeat and PH domain 2b
Synonyms	fe36f09,si:dkey-81b15.3,wu:fe36f09,zgc:158353
General protein information	Preferred Names ArfGAP with SH3 domain, ankyrin repeat and PH domain 2b Names ArfGAP with SH3 domain, ankyrin repeat and PH domain 2b arfGAP with SH3 domain, ankyrin repeat and PH domain 2b ddef2 ddef2b development and differentiation enhancing factor family
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160726.3	XP_005160783.1	arfGAP with SH3 domain, ankyrin repeat and PH domain 2b isoform X1
NM_001044933.2	NP_001038398.2	ArfGAP with SH3 domain, ankyrin repeat and PH domain 2b
XM_005160726.4	XP_005160783.1	arfGAP with SH3 domain, ankyrin repeat and PH domain 2b isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following asap2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the asap2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa33720	NM_001044933.2 Latest version!	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2b (asap2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa25008	XM_005160726.4 Latest version!	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2b (asap2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa25008	XM_005160726.3	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2b (asap2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

asap2b ( NM_001044933.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038398.2 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2b (asap2b), mRNA.

Price & Availability↑

CloneID	ODa33720
Clone ID Related Accession (Same CDS sequence)	NM_001044933.2
Accession Version	NM_001044933.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	ArfGAP with SH3 domain, ankyrin repeat and PH domain 2b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC129209.1. On Jun 3, 2007 this sequence version replaced NM_001044933.1. ##Evidence-Data-START## Transcript exon combination :: BC129209.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCCTGATC AGCTGACAGT GACTGAGTTT GTGGACATTA CCCATGAGGA CTATAAAGCA 
CCGACAACAT CAGTGTTCTG CACGCGCATG GCTCACTGCA GGAATACAGT CGCCGCTCTG 
GAAGAGGCGC TGGATCTGGA CCGCAGTGTA CTGCACAAAA TGAAGAAGTC AGTCAAGGCC 
ATAAACAGCT CTGGTCAGAC TCATGTAGAG AACGAGGAGC AGTACATCCA GGCCATAGAG 
AGGTTTACGG ATAACACTGT GTACAAAGAT GACCCTGAGA TGTCCAATTA CTTCCTCACA 
TTCGCTGGTT TCACCAAGGA GCTTACTGCT CTTTTCAAGA ACTTGCTACA GAACATGAAT 
AACATCATCA CTTTTCCACT AGACAGTCTG CTAAAGGGAG ACCTCAAAGG AGTCAAAGGG 
GATTTGAAAA AGCCATTTGA TAAAGCATGG AAGGATTATG AAACCAAACT GAGCAAGATT 
GAGAAAGAAA AGCGAGAACA TGCCAAACAG CACGGTCTGA TCCGAACAGA GATCAGCGGA 
GGAGAGATCG CAGAAGAGAT GGAGAAAGAG AGACGCCTGT TTCAGCTTCA GATGTGTGAG 
TACCTCATTA AAGTGAATGA AATCAAAGTC AAAAAGGGGG TCGACCTGCT TCACAACCTC 
ATCAAATACT TTCATGCCCA GTGCAATTTC TTTCAGGATG GGCTAAAGGT CGTGGACAAT 
CTGAAACCTT TCATGGAAAA GCTTGCCACA GACTTAACCG CGAACAAACA GACTCAAGAT 
GCAGAAAGGA AACAGTTGCT GCAGCTGAAA GAAACTCTTA AATCTGCTCT ACAGTCTGAG 
TGTAAGGAGG ATGCTCAGTC GAAGCAGAAC GCAGGCTACA GTCTTCACCA GTTGCAGGGC 
AATAAAGCTC ACGGCACGGA GCGCTCTGGG ATGCTCCTCA AACGCAGCGA GGGACTGAGG 
AAAGTTTGGC AGAAAAGGAA GTGCTCTGTG AAAAATGGAT TGTTGACTAT TTCACATGGA 
ACGCCCAATG CACCGCCAGC AAACCTGAAC CTCTTAACCT GCCAAGTGAA GCGTAACCCA 
GATGAGAAAA AATGCTTTGA TCTCATATCA CATGACAGAA CGTATCACTT CCAGACTGAG 
GATGAGGCAG AGTGTCAGGT ATGGGTTTCT GTTCTCCAGA ACAGTAAAGA AGAGGCGCTG 
AACAATGCCT TTAAAGATGA TCAGAATGAG GGAGAAAATA ACATTGTTCG AGAGCTCACT 
AAGGCCATCG TGGGGGAAGT GAAGAAAATG AGCGGCAATG ACGTGTGCTG TGACTGTGGA 
GCTTCCAATC CAACATGGCT CTCCACAAAC CTGGGTGTGT TGATTTGCAT TGAATGTTCT 
GGGATCCATC GGGAAATGGG CGTCCACTAC TCCCGAATAC AGTCTCTGAC ACTGGACCTC 
TTAGGCACAT CTGAACTATT GCTTGCTAAC AGCGTGGGAA ATGCAGCATT CAATGAAATC 
ATGGAAGCAA AACTGTCTTC AGAGATCCCA AAACCCAACC CTTCTAGTGA CATGCAGGTA 
CGAAAAGACT TCATCACAGC CAAATACACA GAGAAGCGTT TCGCTCAGAA GAAGTATGCA 
GATAACGCAG CTCGACTGCA TGCACTGTGT GATGCAGTGA AGTCTCGGGA CATCTTCTCC 
CTGATCCAGG TCTATGCTGA AGGACTGGAC CTGATGGAGA CCATTAATCA GCCTAACCAA 
CATGAACCAG GCGAGACATC ACTACATCTT GCGGTACGAA TGGTGGACCG AAACTCTCTC 
CATATTGTGG ACTTTCTTGT ACAGAACAGT GGCAATTTAG ACAAGCAGAC GGCCAAAGGA 
AGCACAGCGC TGCATTATTG CTGCTTGACT GATAACAGTG AATGTATGAA GCTGCTGCTG 
CGGGGGAAAG CATCTGTCAG CATTACTAAT GATGCTGGAG AGACTGCTCT GGATTTGGCG 
CAGCGTCTCA AACACTCCAA ATGCGAGGAG CTGCTGACTC AGGCGCAGAC GGGGAAGTTC 
AATGTCCATG TGCATGTGGA ATATGACTGG CGTCTGCATA ATGAGGATCT GGACGAGAGC 
GAAGATGAGA TGGAGGACAA GCCCATTCCC ATCAGGCGTG AGGAACGTCC AATAAGCTGT 
ATAGTTCCAG GCAGTGGCCC CATGATGCCC AACATGAGCG CTCTGGCTCG GGACGTGGCC 
AATGTGGTCA ATAATAAGCA GAGGGCTTTT ATTCCGAGCA TGATGATGAA CGAGACTTAC 
GGCACCATGC TCGATCCCAA CTCTCCACCA CTGGGTTTAC CAGGAGTACC TGGCATTCCT 
CTTTTACCCC CTCGGCCCTT GGGAAGGGGA TGGAGTCCAC CAATGGAGAA CATCGGTAGA 
CAGAGGTCAT GTTCAGATCC TGCAAACCCT CAAACTCCTG AACAAAATAA CTCTGTGTAT 
GTTCTGCCTC CTGCTCCTCC ACCTCCTCCT GCACCCAAGA GACCTCCACC TCCAGATCCA 
AAGGCCAGTC TTCTTCCTCC AGCAGCCACG GCTCCTCCTG CACCATCCGC ACCACTCCTT 
ATTCCACCTG CTCCTCTCAG GCCAGCGCCT ATAGTGCCCC CTGCACCAGT TATGCCCACT 
TCATTTTCAT ATAAACCACC ACCTCCACCT GTACCTGTGC CGCCAAAAGC CCCTGCATCC 
AAGCCAGTCA AATCCCCCAC TCGGGATGCC ATTGGTTCAT TTAGTAAAGT TGTTAGTGTA 
AACAAAGCTC CACCCATTGG CCCCCAATCA AGTGATAAAA CAGATGCTCC CTTATCTGCT 
TCAGCACAAG CTACTGCTCC TGTTCCTGCA CCAGAACCTA AACCCAGAAC AGCCCCACCA 
AAACAGAGAC CTAAGAGGGT AAAAGCGTTG TATAACTGTG TGGCTGATAA CGCAGATGAG 
CTGACCTTCT CAGAAGGAGA GATGATAATT GTGGACGGAG AAGAGGACAA GGAGTGGTGG 
GTGGGCCACA TTGATGGGGA ACCAACCAGA AGGGGCGTTT TTCCAGTCGC GTTCGTTCAC 
TTCGTCACTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038398.2
CDS	239..3313
Translation	MPDQLTVTEFVDITHEDYKAPTTSVFCTRMAHCRNTVAALEEALDLDRSVLHKMKKSVKAINSSGQTHVENEEQYIQAIERFTDNTVYKDDPEMSNYFLTFAGFTKELTALFKNLLQNMNNIITFPLDSLLKGDLKGVKGDLKKPFDKAWKDYETKLSKIEKEKREHAKQHGLIRTEISGGEIAEEMEKERRLFQLQMCEYLIKVNEIKVKKGVDLLHNLIKYFHAQCNFFQDGLKVVDNLKPFMEKLATDLTANKQTQDAERKQLLQLKETLKSALQSECKEDAQSKQNAGYSLHQLQGNKAHGTERSGMLLKRSEGLRKVWQKRKCSVKNGLLTISHGTPNAPPANLNLLTCQVKRNPDEKKCFDLISHDRTYHFQTEDEAECQVWVSVLQNSKEEALNNAFKDDQNEGENNIVRELTKAIVGEVKKMSGNDVCCDCGASNPTWLSTNLGVLICIECSGIHREMGVHYSRIQSLTLDLLGTSELLLANSVGNAAFNEIMEAKLSSEIPKPNPSSDMQVRKDFITAKYTEKRFAQKKYADNAARLHALCDAVKSRDIFSLIQVYAEGLDLMETINQPNQHEPGETSLHLAVRMVDRNSLHIVDFLVQNSGNLDKQTAKGSTALHYCCLTDNSECMKLLLRGKASVSITNDAGETALDLAQRLKHSKCEELLTQAQTGKFNVHVHVEYDWRLHNEDLDESEDEMEDKPIPIRREERPISCIVPGSGPMMPNMSALARDVANVVNNKQRAFIPSMMMNETYGTMLDPNSPPLGLPGVPGIPLLPPRPLGRGWSPPMENIGRQRSCSDPANPQTPEQNNSVYVLPPAPPPPPAPKRPPPPDPKASLLPPAATAPPAPSAPLLIPPAPLRPAPIVPPAPVMPTSFSYKPPPPPVPVPPKAPASKPVKSPTRDAIGSFSKVVSVNKAPPIGPQSSDKTDAPLSASAQATAPVPAPEPKPRTAPPKQRPKRVKALYNCVADNADELTFSEGEMIIVDGEEDKEWWVGHIDGEPTRRGVFPVAFVHFVTD

Target ORF information:

RefSeq Version	NM_001044933.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2b (asap2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044933.2

ORF Insert Sequence:

ATGCCTGATC AGCTGACAGT GACTGAGTTT GTGGACATTA CCCATGAGGA CTATAAAGCA 
CCGACAACAT CAGTGTTCTG CACGCGCATG GCTCACTGCA GGAATACAGT CGCCGCTCTG 
GAAGAGGCGC TGGATCTGGA CCGCAGTGTA CTGCACAAAA TGAAGAAGTC AGTCAAGGCC 
ATAAACAGCT CTGGTCAGAC TCATGTAGAG AACGAGGAGC AGTACATCCA GGCCATAGAG 
AGGTTTACGG ATAACACTGT GTACAAAGAT GACCCTGAGA TGTCCAATTA CTTCCTCACA 
TTCGCTGGTT TCACCAAGGA GCTTACTGCT CTTTTCAAGA ACTTGCTACA GAACATGAAT 
AACATCATCA CTTTTCCACT AGACAGTCTG CTAAAGGGAG ACCTCAAAGG AGTCAAAGGG 
GATTTGAAAA AGCCATTTGA TAAAGCATGG AAGGATTATG AAACCAAACT GAGCAAGATT 
GAGAAAGAAA AGCGAGAACA TGCCAAACAG CACGGTCTGA TCCGAACAGA GATCAGCGGA 
GGAGAGATCG CAGAAGAGAT GGAGAAAGAG AGACGCCTGT TTCAGCTTCA GATGTGTGAG 
TACCTCATTA AAGTGAATGA AATCAAAGTC AAAAAGGGGG TCGACCTGCT TCACAACCTC 
ATCAAATACT TTCATGCCCA GTGCAATTTC TTTCAGGATG GGCTAAAGGT CGTGGACAAT 
CTGAAACCTT TCATGGAAAA GCTTGCCACA GACTTAACCG CGAACAAACA GACTCAAGAT 
GCAGAAAGGA AACAGTTGCT GCAGCTGAAA GAAACTCTTA AATCTGCTCT ACAGTCTGAG 
TGTAAGGAGG ATGCTCAGTC GAAGCAGAAC GCAGGCTACA GTCTTCACCA GTTGCAGGGC 
AATAAAGCTC ACGGCACGGA GCGCTCTGGG ATGCTCCTCA AACGCAGCGA GGGACTGAGG 
AAAGTTTGGC AGAAAAGGAA GTGCTCTGTG AAAAATGGAT TGTTGACTAT TTCACATGGA 
ACGCCCAATG CACCGCCAGC AAACCTGAAC CTCTTAACCT GCCAAGTGAA GCGTAACCCA 
GATGAGAAAA AATGCTTTGA TCTCATATCA CATGACAGAA CGTATCACTT CCAGACTGAG 
GATGAGGCAG AGTGTCAGGT ATGGGTTTCT GTTCTCCAGA ACAGTAAAGA AGAGGCGCTG 
AACAATGCCT TTAAAGATGA TCAGAATGAG GGAGAAAATA ACATTGTTCG AGAGCTCACT 
AAGGCCATCG TGGGGGAAGT GAAGAAAATG AGCGGCAATG ACGTGTGCTG TGACTGTGGA 
GCTTCCAATC CAACATGGCT CTCCACAAAC CTGGGTGTGT TGATTTGCAT TGAATGTTCT 
GGGATCCATC GGGAAATGGG CGTCCACTAC TCCCGAATAC AGTCTCTGAC ACTGGACCTC 
TTAGGCACAT CTGAACTATT GCTTGCTAAC AGCGTGGGAA ATGCAGCATT CAATGAAATC 
ATGGAAGCAA AACTGTCTTC AGAGATCCCA AAACCCAACC CTTCTAGTGA CATGCAGGTA 
CGAAAAGACT TCATCACAGC CAAATACACA GAGAAGCGTT TCGCTCAGAA GAAGTATGCA 
GATAACGCAG CTCGACTGCA TGCACTGTGT GATGCAGTGA AGTCTCGGGA CATCTTCTCC 
CTGATCCAGG TCTATGCTGA AGGACTGGAC CTGATGGAGA CCATTAATCA GCCTAACCAA 
CATGAACCAG GCGAGACATC ACTACATCTT GCGGTACGAA TGGTGGACCG AAACTCTCTC 
CATATTGTGG ACTTTCTTGT ACAGAACAGT GGCAATTTAG ACAAGCAGAC GGCCAAAGGA 
AGCACAGCGC TGCATTATTG CTGCTTGACT GATAACAGTG AATGTATGAA GCTGCTGCTG 
CGGGGGAAAG CATCTGTCAG CATTACTAAT GATGCTGGAG AGACTGCTCT GGATTTGGCG 
CAGCGTCTCA AACACTCCAA ATGCGAGGAG CTGCTGACTC AGGCGCAGAC GGGGAAGTTC 
AATGTCCATG TGCATGTGGA ATATGACTGG CGTCTGCATA ATGAGGATCT GGACGAGAGC 
GAAGATGAGA TGGAGGACAA GCCCATTCCC ATCAGGCGTG AGGAACGTCC AATAAGCTGT 
ATAGTTCCAG GCAGTGGCCC CATGATGCCC AACATGAGCG CTCTGGCTCG GGACGTGGCC 
AATGTGGTCA ATAATAAGCA GAGGGCTTTT ATTCCGAGCA TGATGATGAA CGAGACTTAC 
GGCACCATGC TCGATCCCAA CTCTCCACCA CTGGGTTTAC CAGGAGTACC TGGCATTCCT 
CTTTTACCCC CTCGGCCCTT GGGAAGGGGA TGGAGTCCAC CAATGGAGAA CATCGGTAGA 
CAGAGGTCAT GTTCAGATCC TGCAAACCCT CAAACTCCTG AACAAAATAA CTCTGTGTAT 
GTTCTGCCTC CTGCTCCTCC ACCTCCTCCT GCACCCAAGA GACCTCCACC TCCAGATCCA 
AAGGCCAGTC TTCTTCCTCC AGCAGCCACG GCTCCTCCTG CACCATCCGC ACCACTCCTT 
ATTCCACCTG CTCCTCTCAG GCCAGCGCCT ATAGTGCCCC CTGCACCAGT TATGCCCACT 
TCATTTTCAT ATAAACCACC ACCTCCACCT GTACCTGTGC CGCCAAAAGC CCCTGCATCC 
AAGCCAGTCA AATCCCCCAC TCGGGATGCC ATTGGTTCAT TTAGTAAAGT TGTTAGTGTA 
AACAAAGCTC CACCCATTGG CCCCCAATCA AGTGATAAAA CAGATGCTCC CTTATCTGCT 
TCAGCACAAG CTACTGCTCC TGTTCCTGCA CCAGAACCTA AACCCAGAAC AGCCCCACCA 
AAACAGAGAC CTAAGAGGGT AAAAGCGTTG TATAACTGTG TGGCTGATAA CGCAGATGAG 
CTGACCTTCT CAGAAGGAGA GATGATAATT GTGGACGGAG AAGAGGACAA GGAGTGGTGG 
GTGGGCCACA TTGATGGGGA ACCAACCAGA AGGGGCGTTT TTCCAGTCGC GTTCGTTCAC 
TTCGTCACTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap2b ( XM_005160726.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160783.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2b (asap2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25008
Clone ID Related Accession (Same CDS sequence)	XM_005160726.3 , XM_005160726.4
Accession Version	XM_005160726.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	arfGAP with SH3 domain, ankyrin repeat and PH domain 2b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160726.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AGCTGACAGT GACTGAGTTT GTGGATATTA CCCATGAGGA CTATAAAGCA 
CCGACAACAT CAGTGTTCTG CACGCGCATG GCTCACTGCA GGAATACAGT CGCCGCTCTG 
GAAGAGGCGC TGGATCTGGA CCGCAGTGTA CTGCACAAAA TGAAGAAGTC AGTCAAGGCC 
ATAAACAGCT CTGGTCAGAC TCATGTAGAG AACGAGGAGC AGTACATCCA GGCCATAGAG 
AGGTTTACGG ATAACACTGT GTACAAAGAT GACCCTGAGA TGTCCAATTA CTTCCTCACA 
TTCGCTGGTT TCACCAAGGA GCTTACTGCT CTTTTCAAGA ACTTGCTACA GAACATGAAT 
AACATCATCA CTTTTCCACT AGACAGTCTG CTAAAGGGAG ACCTCAAAGG AGTCAAAGGG 
GATTTGAAAA AGCCATTTGA TAAAGCATGG AAGGATTATG AAACCAAACT GAGCAAGATT 
GAGAAAGAAA AGCGAGAACA TGCCAAACAG CACGGTCTGA TCCGAACAGA GATCAGTGGA 
GGAGAGATCG CAGAAGAGAT GGAGAAAGAG AGACGCCTGT TTCAGCTTCA GATGTGTGAG 
TACCTCATTA AAGTGAATGA AATCAAAGTC AAAAAGGGGG TCGACCTGCT TCACAACCTC 
ATCAAATACT TTCATGCCCA GTGCAATTTC TTTCAGGATG GGCTAAAGGT CGTGGACAAT 
CTGAAACCTT TCATGGAAAA GCTTGCCACA GACTTAACCG CGAACAAACA GACTCAAGAT 
GCAGAAAGGA AACAGTTGCT GCAGCTGAAA GAAACTCTTA AATCTGCTCT ACAGTCTGAG 
TGTAAGGAGG ATGCTCAGTC AAAGCAGAAC GCAGGCTACA GTCTTCACCA GTTGCAGGGC 
AATAAAGCTC ACGGCACGGA GCGCTCTGGG ATGCTCCTCA AACGCAGCGA GGGACTGAGG 
AAAGTTTGGC AGAAAAGGAA GTGCTCTGTG AAAAATGGAT TGTTGACTAT TTCACATGGA 
ACGCCCAATG CACCGCCAGC AAACCTGAAC CTCTTAACCT GCCAAGTGAA GCGTAACCCA 
GATGAGAAAA AATGCTTTGA TCTCATATCA CATGACAGAA CGTATCACTT CCAGACTGAG 
GATGAGGCAG AGTGTCAGGT ATGGGTTTCT GTTCTCCAGA ACAGTAAAGA AGAGGCGCTG 
AACAATGCCT TTAAAGATGA TCAGAATGAG GGAGAAAATA ACATTGTTCG AGAGCTCACT 
AAGGCCATCG TGGGGGAAGT GAAGAAAATG AGCGGCAATG ACGTGTGCTG TGACTGTGGA 
GCTTCCAATC CAACATGGCT CTCCACAAAC CTGGGTGTGT TGATTTGCAT TGAATGCTCT 
GGGATCCATC GGGAAATGGG CGTCCACTAC TCCCGAATAC AGTCTCTGAC ACTGGACCTC 
TTAGGCACAT CTGAACTATT GCTTGCTAAC AGTGTGGGAA ATGCAGCATT CAATGAAATC 
ATGGAAGCAA AACTGTCTTC AGAGATCCCA AAACCCAACC CTTCTAGTGA CATGCAGGTA 
CGAAAAGACT TCATCACAGC CAAATACACA GAGAAGCGTT TCGCTCAGAA GAAGTATGCA 
GATAACGCAG CTCGACTGCA TGCACTGTGT GATGCAGTGA AGTCTCGGGA CATCTTCTCC 
CTGATCCAGG TCTATGCTGA AGGACTGGAC CTGATGGAGA CCATTAATCA GCCTAACCAA 
CATGAACCAG GCGAGACATC ACTACATCTT GCGGTACGAA TGGTGGACCG AAACTCCCTC 
CATATTGTGG ACTTTCTTGT ACAGAACAGT GGCAATTTAG ACAAGCAGAC AGCCAAAGGA 
AGCACAGCGC TACATTATTG CTGCTTGACT GATAACAGTG AATGTATGAA GCTGCTGCTG 
CGGGGGAAAG CATCTGTCAG CATTACTAAT GATGCTGGAG AGACTGCTCT GGATTTGGCG 
CAGCGTCTCA AACACTCCAA ATGCGAGGAG CTGCTGACTC AGGCGCAGAC GGGGAAGTTC 
AATGTCCATG TGCATGTGGA ATATGACTGG CGTCTGCATA ATGAGGATCT GGACGAGAGC 
GAAGATGAGA TGGAGGACAA GCCCATTCCC ATCAGGCGTG AGGAGCGTCC AATAAGCTGT 
ATAGTTCCAG GCAGTGGCCC CATGATGCCC AACATGAGCG CTCTGGCTCG GGACGTGGCC 
AATGTGGTCA ATAATAAGCA GAGGGCTTTT ATTCCGAGCA TGATGATGAA CGAGACTTAC 
GGCACCATGC TCGATCCCAA CTCTCCACCA CTGGGTTTAC CAGGAGTACC TGGCATTCCT 
CTTTTACCCC CTCGGCCCTT GGGAAGGGGA TGGAGTCCAC CAATGGAGAA CATCGGTAGA 
CAGAGGTCAT GTTCAGATCC TGCAAACCCT CAAACTCCTG AACAAAATAA CTCTGTGTAT 
GTTCTGCCTC CTGCTCCTCC ACCTCCTCCT GCACCCAAGA GACCTCCACC TCCAGATCCA 
AAGGCCAGTC TTCTTCCTCC AGCAGCCACG GCTCCTCCTG CACCATCCGC ACCGCTCCTT 
ATTCCACCTG CTCCTCTCAG GCCAGCGCCT GTAGTGCCCC CTGCACCAGT TATGCCCACT 
TCATTTTCAT ATAAACCACC ACCTCCACCT GTACCTGTAC AGCCAAAAGC CCCTGCATCC 
AAGCCAGTCA AATCCCCCAC TCGGGATGCC ATTGGTTCAT TTAGTAAAAT TGTAAGTGTA 
AACAAAGCTC CACCCATTGG CCCCCAATCA AGTGATAAAA CAGCACAAGC TACTGCTCCT 
GTTCCTGCAC CAGAACCTAA ACCCAGAACA GCCCCACCAA AACAGAGACC TAAGAGGGTA 
AAGGCGTTGT ATAACTGTGT GGCTGATAAC GCAGATGAAC TGACCTTCTC AGAAGGAGAG 
ATGATAATTG TGGACGGAGA AGAGGACAAG GAGTGGTGGG TGGGCCACAT TGATGGGGAA 
CCAACCAGAA GGGGCGTTTT TCCAGTCGCG TTCGTTCACT TCGTCACTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160783.1
CDS	276..3329
Translation	MPDQLTVTEFVDITHEDYKAPTTSVFCTRMAHCRNTVAALEEALDLDRSVLHKMKKSVKAINSSGQTHVENEEQYIQAIERFTDNTVYKDDPEMSNYFLTFAGFTKELTALFKNLLQNMNNIITFPLDSLLKGDLKGVKGDLKKPFDKAWKDYETKLSKIEKEKREHAKQHGLIRTEISGGEIAEEMEKERRLFQLQMCEYLIKVNEIKVKKGVDLLHNLIKYFHAQCNFFQDGLKVVDNLKPFMEKLATDLTANKQTQDAERKQLLQLKETLKSALQSECKEDAQSKQNAGYSLHQLQGNKAHGTERSGMLLKRSEGLRKVWQKRKCSVKNGLLTISHGTPNAPPANLNLLTCQVKRNPDEKKCFDLISHDRTYHFQTEDEAECQVWVSVLQNSKEEALNNAFKDDQNEGENNIVRELTKAIVGEVKKMSGNDVCCDCGASNPTWLSTNLGVLICIECSGIHREMGVHYSRIQSLTLDLLGTSELLLANSVGNAAFNEIMEAKLSSEIPKPNPSSDMQVRKDFITAKYTEKRFAQKKYADNAARLHALCDAVKSRDIFSLIQVYAEGLDLMETINQPNQHEPGETSLHLAVRMVDRNSLHIVDFLVQNSGNLDKQTAKGSTALHYCCLTDNSECMKLLLRGKASVSITNDAGETALDLAQRLKHSKCEELLTQAQTGKFNVHVHVEYDWRLHNEDLDESEDEMEDKPIPIRREERPISCIVPGSGPMMPNMSALARDVANVVNNKQRAFIPSMMMNETYGTMLDPNSPPLGLPGVPGIPLLPPRPLGRGWSPPMENIGRQRSCSDPANPQTPEQNNSVYVLPPAPPPPPAPKRPPPPDPKASLLPPAATAPPAPSAPLLIPPAPLRPAPVVPPAPVMPTSFSYKPPPPPVPVQPKAPASKPVKSPTRDAIGSFSKIVSVNKAPPIGPQSSDKTAQATAPVPAPEPKPRTAPPKQRPKRVKALYNCVADNADELTFSEGEMIIVDGEEDKEWWVGHIDGEPTRRGVFPVAFVHFVTD

Target ORF information:

RefSeq Version	XM_005160726.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2b (asap2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160726.3

ORF Insert Sequence:

ATGCCTGATC AGCTGACAGT GACTGAGTTT GTGGATATTA CCCATGAGGA CTATAAAGCA 
CCGACAACAT CAGTGTTCTG CACGCGCATG GCTCACTGCA GGAATACAGT CGCCGCTCTG 
GAAGAGGCGC TGGATCTGGA CCGCAGTGTA CTGCACAAAA TGAAGAAGTC AGTCAAGGCC 
ATAAACAGCT CTGGTCAGAC TCATGTAGAG AACGAGGAGC AGTACATCCA GGCCATAGAG 
AGGTTTACGG ATAACACTGT GTACAAAGAT GACCCTGAGA TGTCCAATTA CTTCCTCACA 
TTCGCTGGTT TCACCAAGGA GCTTACTGCT CTTTTCAAGA ACTTGCTACA GAACATGAAT 
AACATCATCA CTTTTCCACT AGACAGTCTG CTAAAGGGAG ACCTCAAAGG AGTCAAAGGG 
GATTTGAAAA AGCCATTTGA TAAAGCATGG AAGGATTATG AAACCAAACT GAGCAAGATT 
GAGAAAGAAA AGCGAGAACA TGCCAAACAG CACGGTCTGA TCCGAACAGA GATCAGTGGA 
GGAGAGATCG CAGAAGAGAT GGAGAAAGAG AGACGCCTGT TTCAGCTTCA GATGTGTGAG 
TACCTCATTA AAGTGAATGA AATCAAAGTC AAAAAGGGGG TCGACCTGCT TCACAACCTC 
ATCAAATACT TTCATGCCCA GTGCAATTTC TTTCAGGATG GGCTAAAGGT CGTGGACAAT 
CTGAAACCTT TCATGGAAAA GCTTGCCACA GACTTAACCG CGAACAAACA GACTCAAGAT 
GCAGAAAGGA AACAGTTGCT GCAGCTGAAA GAAACTCTTA AATCTGCTCT ACAGTCTGAG 
TGTAAGGAGG ATGCTCAGTC AAAGCAGAAC GCAGGCTACA GTCTTCACCA GTTGCAGGGC 
AATAAAGCTC ACGGCACGGA GCGCTCTGGG ATGCTCCTCA AACGCAGCGA GGGACTGAGG 
AAAGTTTGGC AGAAAAGGAA GTGCTCTGTG AAAAATGGAT TGTTGACTAT TTCACATGGA 
ACGCCCAATG CACCGCCAGC AAACCTGAAC CTCTTAACCT GCCAAGTGAA GCGTAACCCA 
GATGAGAAAA AATGCTTTGA TCTCATATCA CATGACAGAA CGTATCACTT CCAGACTGAG 
GATGAGGCAG AGTGTCAGGT ATGGGTTTCT GTTCTCCAGA ACAGTAAAGA AGAGGCGCTG 
AACAATGCCT TTAAAGATGA TCAGAATGAG GGAGAAAATA ACATTGTTCG AGAGCTCACT 
AAGGCCATCG TGGGGGAAGT GAAGAAAATG AGCGGCAATG ACGTGTGCTG TGACTGTGGA 
GCTTCCAATC CAACATGGCT CTCCACAAAC CTGGGTGTGT TGATTTGCAT TGAATGCTCT 
GGGATCCATC GGGAAATGGG CGTCCACTAC TCCCGAATAC AGTCTCTGAC ACTGGACCTC 
TTAGGCACAT CTGAACTATT GCTTGCTAAC AGTGTGGGAA ATGCAGCATT CAATGAAATC 
ATGGAAGCAA AACTGTCTTC AGAGATCCCA AAACCCAACC CTTCTAGTGA CATGCAGGTA 
CGAAAAGACT TCATCACAGC CAAATACACA GAGAAGCGTT TCGCTCAGAA GAAGTATGCA 
GATAACGCAG CTCGACTGCA TGCACTGTGT GATGCAGTGA AGTCTCGGGA CATCTTCTCC 
CTGATCCAGG TCTATGCTGA AGGACTGGAC CTGATGGAGA CCATTAATCA GCCTAACCAA 
CATGAACCAG GCGAGACATC ACTACATCTT GCGGTACGAA TGGTGGACCG AAACTCCCTC 
CATATTGTGG ACTTTCTTGT ACAGAACAGT GGCAATTTAG ACAAGCAGAC AGCCAAAGGA 
AGCACAGCGC TACATTATTG CTGCTTGACT GATAACAGTG AATGTATGAA GCTGCTGCTG 
CGGGGGAAAG CATCTGTCAG CATTACTAAT GATGCTGGAG AGACTGCTCT GGATTTGGCG 
CAGCGTCTCA AACACTCCAA ATGCGAGGAG CTGCTGACTC AGGCGCAGAC GGGGAAGTTC 
AATGTCCATG TGCATGTGGA ATATGACTGG CGTCTGCATA ATGAGGATCT GGACGAGAGC 
GAAGATGAGA TGGAGGACAA GCCCATTCCC ATCAGGCGTG AGGAGCGTCC AATAAGCTGT 
ATAGTTCCAG GCAGTGGCCC CATGATGCCC AACATGAGCG CTCTGGCTCG GGACGTGGCC 
AATGTGGTCA ATAATAAGCA GAGGGCTTTT ATTCCGAGCA TGATGATGAA CGAGACTTAC 
GGCACCATGC TCGATCCCAA CTCTCCACCA CTGGGTTTAC CAGGAGTACC TGGCATTCCT 
CTTTTACCCC CTCGGCCCTT GGGAAGGGGA TGGAGTCCAC CAATGGAGAA CATCGGTAGA 
CAGAGGTCAT GTTCAGATCC TGCAAACCCT CAAACTCCTG AACAAAATAA CTCTGTGTAT 
GTTCTGCCTC CTGCTCCTCC ACCTCCTCCT GCACCCAAGA GACCTCCACC TCCAGATCCA 
AAGGCCAGTC TTCTTCCTCC AGCAGCCACG GCTCCTCCTG CACCATCCGC ACCGCTCCTT 
ATTCCACCTG CTCCTCTCAG GCCAGCGCCT GTAGTGCCCC CTGCACCAGT TATGCCCACT 
TCATTTTCAT ATAAACCACC ACCTCCACCT GTACCTGTAC AGCCAAAAGC CCCTGCATCC 
AAGCCAGTCA AATCCCCCAC TCGGGATGCC ATTGGTTCAT TTAGTAAAAT TGTAAGTGTA 
AACAAAGCTC CACCCATTGG CCCCCAATCA AGTGATAAAA CAGCACAAGC TACTGCTCCT 
GTTCCTGCAC CAGAACCTAA ACCCAGAACA GCCCCACCAA AACAGAGACC TAAGAGGGTA 
AAGGCGTTGT ATAACTGTGT GGCTGATAAC GCAGATGAAC TGACCTTCTC AGAAGGAGAG 
ATGATAATTG TGGACGGAGA AGAGGACAAG GAGTGGTGGG TGGGCCACAT TGATGGGGAA 
CCAACCAGAA GGGGCGTTTT TCCAGTCGCG TTCGTTCACT TCGTCACTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap2b ( XM_005160726.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160783.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2b (asap2b), transcript variant X1, mRNA.

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CloneID	ODa25008
Clone ID Related Accession (Same CDS sequence)	XM_005160726.3 , XM_005160726.4
Accession Version	XM_005160726.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	arfGAP with SH3 domain, ankyrin repeat and PH domain 2b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160726.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AGCTGACAGT GACTGAGTTT GTGGATATTA CCCATGAGGA CTATAAAGCA 
CCGACAACAT CAGTGTTCTG CACGCGCATG GCTCACTGCA GGAATACAGT CGCCGCTCTG 
GAAGAGGCGC TGGATCTGGA CCGCAGTGTA CTGCACAAAA TGAAGAAGTC AGTCAAGGCC 
ATAAACAGCT CTGGTCAGAC TCATGTAGAG AACGAGGAGC AGTACATCCA GGCCATAGAG 
AGGTTTACGG ATAACACTGT GTACAAAGAT GACCCTGAGA TGTCCAATTA CTTCCTCACA 
TTCGCTGGTT TCACCAAGGA GCTTACTGCT CTTTTCAAGA ACTTGCTACA GAACATGAAT 
AACATCATCA CTTTTCCACT AGACAGTCTG CTAAAGGGAG ACCTCAAAGG AGTCAAAGGG 
GATTTGAAAA AGCCATTTGA TAAAGCATGG AAGGATTATG AAACCAAACT GAGCAAGATT 
GAGAAAGAAA AGCGAGAACA TGCCAAACAG CACGGTCTGA TCCGAACAGA GATCAGTGGA 
GGAGAGATCG CAGAAGAGAT GGAGAAAGAG AGACGCCTGT TTCAGCTTCA GATGTGTGAG 
TACCTCATTA AAGTGAATGA AATCAAAGTC AAAAAGGGGG TCGACCTGCT TCACAACCTC 
ATCAAATACT TTCATGCCCA GTGCAATTTC TTTCAGGATG GGCTAAAGGT CGTGGACAAT 
CTGAAACCTT TCATGGAAAA GCTTGCCACA GACTTAACCG CGAACAAACA GACTCAAGAT 
GCAGAAAGGA AACAGTTGCT GCAGCTGAAA GAAACTCTTA AATCTGCTCT ACAGTCTGAG 
TGTAAGGAGG ATGCTCAGTC AAAGCAGAAC GCAGGCTACA GTCTTCACCA GTTGCAGGGC 
AATAAAGCTC ACGGCACGGA GCGCTCTGGG ATGCTCCTCA AACGCAGCGA GGGACTGAGG 
AAAGTTTGGC AGAAAAGGAA GTGCTCTGTG AAAAATGGAT TGTTGACTAT TTCACATGGA 
ACGCCCAATG CACCGCCAGC AAACCTGAAC CTCTTAACCT GCCAAGTGAA GCGTAACCCA 
GATGAGAAAA AATGCTTTGA TCTCATATCA CATGACAGAA CGTATCACTT CCAGACTGAG 
GATGAGGCAG AGTGTCAGGT ATGGGTTTCT GTTCTCCAGA ACAGTAAAGA AGAGGCGCTG 
AACAATGCCT TTAAAGATGA TCAGAATGAG GGAGAAAATA ACATTGTTCG AGAGCTCACT 
AAGGCCATCG TGGGGGAAGT GAAGAAAATG AGCGGCAATG ACGTGTGCTG TGACTGTGGA 
GCTTCCAATC CAACATGGCT CTCCACAAAC CTGGGTGTGT TGATTTGCAT TGAATGCTCT 
GGGATCCATC GGGAAATGGG CGTCCACTAC TCCCGAATAC AGTCTCTGAC ACTGGACCTC 
TTAGGCACAT CTGAACTATT GCTTGCTAAC AGTGTGGGAA ATGCAGCATT CAATGAAATC 
ATGGAAGCAA AACTGTCTTC AGAGATCCCA AAACCCAACC CTTCTAGTGA CATGCAGGTA 
CGAAAAGACT TCATCACAGC CAAATACACA GAGAAGCGTT TCGCTCAGAA GAAGTATGCA 
GATAACGCAG CTCGACTGCA TGCACTGTGT GATGCAGTGA AGTCTCGGGA CATCTTCTCC 
CTGATCCAGG TCTATGCTGA AGGACTGGAC CTGATGGAGA CCATTAATCA GCCTAACCAA 
CATGAACCAG GCGAGACATC ACTACATCTT GCGGTACGAA TGGTGGACCG AAACTCCCTC 
CATATTGTGG ACTTTCTTGT ACAGAACAGT GGCAATTTAG ACAAGCAGAC AGCCAAAGGA 
AGCACAGCGC TACATTATTG CTGCTTGACT GATAACAGTG AATGTATGAA GCTGCTGCTG 
CGGGGGAAAG CATCTGTCAG CATTACTAAT GATGCTGGAG AGACTGCTCT GGATTTGGCG 
CAGCGTCTCA AACACTCCAA ATGCGAGGAG CTGCTGACTC AGGCGCAGAC GGGGAAGTTC 
AATGTCCATG TGCATGTGGA ATATGACTGG CGTCTGCATA ATGAGGATCT GGACGAGAGC 
GAAGATGAGA TGGAGGACAA GCCCATTCCC ATCAGGCGTG AGGAGCGTCC AATAAGCTGT 
ATAGTTCCAG GCAGTGGCCC CATGATGCCC AACATGAGCG CTCTGGCTCG GGACGTGGCC 
AATGTGGTCA ATAATAAGCA GAGGGCTTTT ATTCCGAGCA TGATGATGAA CGAGACTTAC 
GGCACCATGC TCGATCCCAA CTCTCCACCA CTGGGTTTAC CAGGAGTACC TGGCATTCCT 
CTTTTACCCC CTCGGCCCTT GGGAAGGGGA TGGAGTCCAC CAATGGAGAA CATCGGTAGA 
CAGAGGTCAT GTTCAGATCC TGCAAACCCT CAAACTCCTG AACAAAATAA CTCTGTGTAT 
GTTCTGCCTC CTGCTCCTCC ACCTCCTCCT GCACCCAAGA GACCTCCACC TCCAGATCCA 
AAGGCCAGTC TTCTTCCTCC AGCAGCCACG GCTCCTCCTG CACCATCCGC ACCGCTCCTT 
ATTCCACCTG CTCCTCTCAG GCCAGCGCCT GTAGTGCCCC CTGCACCAGT TATGCCCACT 
TCATTTTCAT ATAAACCACC ACCTCCACCT GTACCTGTAC AGCCAAAAGC CCCTGCATCC 
AAGCCAGTCA AATCCCCCAC TCGGGATGCC ATTGGTTCAT TTAGTAAAAT TGTAAGTGTA 
AACAAAGCTC CACCCATTGG CCCCCAATCA AGTGATAAAA CAGCACAAGC TACTGCTCCT 
GTTCCTGCAC CAGAACCTAA ACCCAGAACA GCCCCACCAA AACAGAGACC TAAGAGGGTA 
AAGGCGTTGT ATAACTGTGT GGCTGATAAC GCAGATGAAC TGACCTTCTC AGAAGGAGAG 
ATGATAATTG TGGACGGAGA AGAGGACAAG GAGTGGTGGG TGGGCCACAT TGATGGGGAA 
CCAACCAGAA GGGGCGTTTT TCCAGTCGCG TTCGTTCACT TCGTCACTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160783.1
CDS	282..3335
Translation	MPDQLTVTEFVDITHEDYKAPTTSVFCTRMAHCRNTVAALEEALDLDRSVLHKMKKSVKAINSSGQTHVENEEQYIQAIERFTDNTVYKDDPEMSNYFLTFAGFTKELTALFKNLLQNMNNIITFPLDSLLKGDLKGVKGDLKKPFDKAWKDYETKLSKIEKEKREHAKQHGLIRTEISGGEIAEEMEKERRLFQLQMCEYLIKVNEIKVKKGVDLLHNLIKYFHAQCNFFQDGLKVVDNLKPFMEKLATDLTANKQTQDAERKQLLQLKETLKSALQSECKEDAQSKQNAGYSLHQLQGNKAHGTERSGMLLKRSEGLRKVWQKRKCSVKNGLLTISHGTPNAPPANLNLLTCQVKRNPDEKKCFDLISHDRTYHFQTEDEAECQVWVSVLQNSKEEALNNAFKDDQNEGENNIVRELTKAIVGEVKKMSGNDVCCDCGASNPTWLSTNLGVLICIECSGIHREMGVHYSRIQSLTLDLLGTSELLLANSVGNAAFNEIMEAKLSSEIPKPNPSSDMQVRKDFITAKYTEKRFAQKKYADNAARLHALCDAVKSRDIFSLIQVYAEGLDLMETINQPNQHEPGETSLHLAVRMVDRNSLHIVDFLVQNSGNLDKQTAKGSTALHYCCLTDNSECMKLLLRGKASVSITNDAGETALDLAQRLKHSKCEELLTQAQTGKFNVHVHVEYDWRLHNEDLDESEDEMEDKPIPIRREERPISCIVPGSGPMMPNMSALARDVANVVNNKQRAFIPSMMMNETYGTMLDPNSPPLGLPGVPGIPLLPPRPLGRGWSPPMENIGRQRSCSDPANPQTPEQNNSVYVLPPAPPPPPAPKRPPPPDPKASLLPPAATAPPAPSAPLLIPPAPLRPAPVVPPAPVMPTSFSYKPPPPPVPVQPKAPASKPVKSPTRDAIGSFSKIVSVNKAPPIGPQSSDKTAQATAPVPAPEPKPRTAPPKQRPKRVKALYNCVADNADELTFSEGEMIIVDGEEDKEWWVGHIDGEPTRRGVFPVAFVHFVTD

Target ORF information:

RefSeq Version	XM_005160726.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2b (asap2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160726.4

ORF Insert Sequence:

ATGCCTGATC AGCTGACAGT GACTGAGTTT GTGGATATTA CCCATGAGGA CTATAAAGCA 
CCGACAACAT CAGTGTTCTG CACGCGCATG GCTCACTGCA GGAATACAGT CGCCGCTCTG 
GAAGAGGCGC TGGATCTGGA CCGCAGTGTA CTGCACAAAA TGAAGAAGTC AGTCAAGGCC 
ATAAACAGCT CTGGTCAGAC TCATGTAGAG AACGAGGAGC AGTACATCCA GGCCATAGAG 
AGGTTTACGG ATAACACTGT GTACAAAGAT GACCCTGAGA TGTCCAATTA CTTCCTCACA 
TTCGCTGGTT TCACCAAGGA GCTTACTGCT CTTTTCAAGA ACTTGCTACA GAACATGAAT 
AACATCATCA CTTTTCCACT AGACAGTCTG CTAAAGGGAG ACCTCAAAGG AGTCAAAGGG 
GATTTGAAAA AGCCATTTGA TAAAGCATGG AAGGATTATG AAACCAAACT GAGCAAGATT 
GAGAAAGAAA AGCGAGAACA TGCCAAACAG CACGGTCTGA TCCGAACAGA GATCAGTGGA 
GGAGAGATCG CAGAAGAGAT GGAGAAAGAG AGACGCCTGT TTCAGCTTCA GATGTGTGAG 
TACCTCATTA AAGTGAATGA AATCAAAGTC AAAAAGGGGG TCGACCTGCT TCACAACCTC 
ATCAAATACT TTCATGCCCA GTGCAATTTC TTTCAGGATG GGCTAAAGGT CGTGGACAAT 
CTGAAACCTT TCATGGAAAA GCTTGCCACA GACTTAACCG CGAACAAACA GACTCAAGAT 
GCAGAAAGGA AACAGTTGCT GCAGCTGAAA GAAACTCTTA AATCTGCTCT ACAGTCTGAG 
TGTAAGGAGG ATGCTCAGTC AAAGCAGAAC GCAGGCTACA GTCTTCACCA GTTGCAGGGC 
AATAAAGCTC ACGGCACGGA GCGCTCTGGG ATGCTCCTCA AACGCAGCGA GGGACTGAGG 
AAAGTTTGGC AGAAAAGGAA GTGCTCTGTG AAAAATGGAT TGTTGACTAT TTCACATGGA 
ACGCCCAATG CACCGCCAGC AAACCTGAAC CTCTTAACCT GCCAAGTGAA GCGTAACCCA 
GATGAGAAAA AATGCTTTGA TCTCATATCA CATGACAGAA CGTATCACTT CCAGACTGAG 
GATGAGGCAG AGTGTCAGGT ATGGGTTTCT GTTCTCCAGA ACAGTAAAGA AGAGGCGCTG 
AACAATGCCT TTAAAGATGA TCAGAATGAG GGAGAAAATA ACATTGTTCG AGAGCTCACT 
AAGGCCATCG TGGGGGAAGT GAAGAAAATG AGCGGCAATG ACGTGTGCTG TGACTGTGGA 
GCTTCCAATC CAACATGGCT CTCCACAAAC CTGGGTGTGT TGATTTGCAT TGAATGCTCT 
GGGATCCATC GGGAAATGGG CGTCCACTAC TCCCGAATAC AGTCTCTGAC ACTGGACCTC 
TTAGGCACAT CTGAACTATT GCTTGCTAAC AGTGTGGGAA ATGCAGCATT CAATGAAATC 
ATGGAAGCAA AACTGTCTTC AGAGATCCCA AAACCCAACC CTTCTAGTGA CATGCAGGTA 
CGAAAAGACT TCATCACAGC CAAATACACA GAGAAGCGTT TCGCTCAGAA GAAGTATGCA 
GATAACGCAG CTCGACTGCA TGCACTGTGT GATGCAGTGA AGTCTCGGGA CATCTTCTCC 
CTGATCCAGG TCTATGCTGA AGGACTGGAC CTGATGGAGA CCATTAATCA GCCTAACCAA 
CATGAACCAG GCGAGACATC ACTACATCTT GCGGTACGAA TGGTGGACCG AAACTCCCTC 
CATATTGTGG ACTTTCTTGT ACAGAACAGT GGCAATTTAG ACAAGCAGAC AGCCAAAGGA 
AGCACAGCGC TACATTATTG CTGCTTGACT GATAACAGTG AATGTATGAA GCTGCTGCTG 
CGGGGGAAAG CATCTGTCAG CATTACTAAT GATGCTGGAG AGACTGCTCT GGATTTGGCG 
CAGCGTCTCA AACACTCCAA ATGCGAGGAG CTGCTGACTC AGGCGCAGAC GGGGAAGTTC 
AATGTCCATG TGCATGTGGA ATATGACTGG CGTCTGCATA ATGAGGATCT GGACGAGAGC 
GAAGATGAGA TGGAGGACAA GCCCATTCCC ATCAGGCGTG AGGAGCGTCC AATAAGCTGT 
ATAGTTCCAG GCAGTGGCCC CATGATGCCC AACATGAGCG CTCTGGCTCG GGACGTGGCC 
AATGTGGTCA ATAATAAGCA GAGGGCTTTT ATTCCGAGCA TGATGATGAA CGAGACTTAC 
GGCACCATGC TCGATCCCAA CTCTCCACCA CTGGGTTTAC CAGGAGTACC TGGCATTCCT 
CTTTTACCCC CTCGGCCCTT GGGAAGGGGA TGGAGTCCAC CAATGGAGAA CATCGGTAGA 
CAGAGGTCAT GTTCAGATCC TGCAAACCCT CAAACTCCTG AACAAAATAA CTCTGTGTAT 
GTTCTGCCTC CTGCTCCTCC ACCTCCTCCT GCACCCAAGA GACCTCCACC TCCAGATCCA 
AAGGCCAGTC TTCTTCCTCC AGCAGCCACG GCTCCTCCTG CACCATCCGC ACCGCTCCTT 
ATTCCACCTG CTCCTCTCAG GCCAGCGCCT GTAGTGCCCC CTGCACCAGT TATGCCCACT 
TCATTTTCAT ATAAACCACC ACCTCCACCT GTACCTGTAC AGCCAAAAGC CCCTGCATCC 
AAGCCAGTCA AATCCCCCAC TCGGGATGCC ATTGGTTCAT TTAGTAAAAT TGTAAGTGTA 
AACAAAGCTC CACCCATTGG CCCCCAATCA AGTGATAAAA CAGCACAAGC TACTGCTCCT 
GTTCCTGCAC CAGAACCTAA ACCCAGAACA GCCCCACCAA AACAGAGACC TAAGAGGGTA 
AAGGCGTTGT ATAACTGTGT GGCTGATAAC GCAGATGAAC TGACCTTCTC AGAAGGAGAG 
ATGATAATTG TGGACGGAGA AGAGGACAAG GAGTGGTGGG TGGGCCACAT TGATGGGGAA 
CCAACCAGAA GGGGCGTTTT TCCAGTCGCG TTCGTTCACT TCGTCACTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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asap2b cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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