COL27A1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL27A1B
Entrez Gene ID	560145
Full Name	collagen, type XXVII, alpha 1b
Synonyms	col27a1
General protein information	Preferred Names collagen, type XXVII, alpha 1b Names collagen alpha-1(XXVII) chain B novel collagen family protein
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017356118.1	XP_017211607.1	collagen alpha-1(XXVII) chain B isoform X1
XM_017356118.2	XP_017211607.1	collagen alpha-1(XXVII) chain B isoform X1
XM_017356119.1	XP_017211608.1	collagen alpha-1(XXVII) chain B isoform X2
XM_021476201.1	XP_021331876.1	collagen alpha-1(XXVII) chain B isoform X2
NM_001080575.1	NP_001074044.1	collagen alpha-1(XXVII) chain B precursor
XM_009301947.1	XP_009300222.1	collagen alpha-1(XXVII) chain B isoform X3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	col27a1b	NP_001074044.1
Danio rerio (zebrafish)	col27a1a	NP_001156766.1

Gene Ontology
Bibliography

Related articles in PubMed

Fishing for collagen function: About development, regeneration and disease.
Bretaud S, Nauroy P, Malbouyres M, Ruggiero F
Seminars in cell & developmental biology89100-108(2019 05)

Collagen duplicate genes of bone and cartilage participate during regeneration of zebrafish fin skeleton.
Duran I, Csukasi F, Taylor SP, Krakow D, Becerra J, Bombarely A, Marí-Beffa M
Gene expression patterns : GEP19(1-2)60-9(2015 Sep-Nov)

Critical early roles for col27a1a and col27a1b in zebrafish notochord morphogenesis, vertebral mineralization and post-embryonic axial growth.
Christiansen HE, Lang MR, Pace JM, Parichy DM
PloS one4(12)e8481(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following COL27A1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL27A1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa142658	XM_021476201.1 Latest version!	Danio rerio collagen, type XXVII, alpha 1b (col27a1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa43244	XM_009301947.1 Latest version!	Danio rerio collagen, type XXVII, alpha 1b (col27a1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa59099	XM_017356118.2 Latest version!	Danio rerio collagen, type XXVII, alpha 1b (col27a1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa59100	XM_017356119.1 Latest version!	Danio rerio collagen, type XXVII, alpha 1b (col27a1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa38392	NM_001080575.1 Latest version!	Danio rerio collagen, type XXVII, alpha 1b (col27a1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
ODa59099	XM_017356118.1	Danio rerio collagen, type XXVII, alpha 1b (col27a1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL27A1B ( XM_021476201.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021331876.1 Danio rerio collagen, type XXVII, alpha 1b (col27a1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa142658
Clone ID Related Accession (Same CDS sequence)	XM_021476201.1
Accession Version	XM_021476201.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(XXVII) chain B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGAG AGCCTGGAAT GACTGGAAAT CCTGGGATGC TTGGTGAGAG GGGTCCTGTT 
GGTTTCGTCG GGCCTTTTGG TGAGATGGGA CTTGCTGGAG AAAAGGGAGA TCGTGGTGAG 
ACGGGTCAGC CTGGGCCACC TGGAGAAAAG GGAGCCATGG GTCACCCAGG GGCTCCAGGA 
GAGAGAGGCC TGTCTGGACC AGCAGGTGCT CCAGGGCCTC ATGGATCTAG GGGCCTCAGT 
GGCACCAGGG GGCCAAAAGG CACAAGGGGT GCAAGGGGAC CAGATGGACC AGTTGGAGAG 
AAAGGAATGA TGGGAATGAA GGGTCCTGAA GGTCCACCAG GAAAACAAGG TTTAAGCGGA 
CAAATGGGTA AAATTGGAGA AACTGGTGAA GCAGGACCTA CCGGATTTAC TGGTGTTCAA 
GGACCAACTG GACCTCCTGG AGCAAAAGGC ATCTTAGGAG AACCTGGTCC TCAAGGATCA 
CCGGGAGTGT TGGGTCCATT GGGTGAGATT GGAGCAATAG GTTTACCTGG GAAGGCTGGC 
GATCAGGGCT TACCGGGAGA ACCAGGAGAG AAGGGTGCAG TCGGGTCTCC TGGGAATATT 
GGAGAGCAAG GCCTGATTGG ACCCCGAGGT GACCCTGGAG TCGATGGAGA GGCTGGTCCA 
AGTGGTCCCG ATGGAGCCAA GGGTGAACAG GGGGATGTTG GACTGGAGGG AGAATCAGGC 
GAAAAAGGAG TCATCGGATT TAAAGGAACA GAGGGACGTA CTGGTGATCC TGGCTTAATA 
GGCGTCAAGG GCCCTGAAGG CAAAATAGGG AAAACTGGTG AGAGGGGAAA ACCTGGAGAG 
AAGGGAAGCA AAGGTCATCA AGGTCAACTT GGCGAGATGG GTGCTTTGGG TGAGCAAGGT 
GACACGGGTT TCATCGGGCC AAAAGGAAGC AGAGGAACTA CTGGATTTAT GGGTGCGACG 
GGAAAAATGG GTCAGCACGG AGAGCCAGGT TTGGTGGGAT ATGAAGGTCA TCAAGGGCCA 
CAAGGTTCAT TGGGATCCCC GGGACCAAAA GGTGAAAAGG GAGAGCAAGG CGATGACGGG 
AAGGTTGAAG GTCCACCTGG TCCTTCTGGT GACATCGGAC CTGTTGGCAA CAGAGGAGAC 
AGAGGAGAAC CTGGTGACCC TGGATACCCA GGCCTTGAAG GTGTTCAAGG AGAAAGAGGA 
AAAGAGGGGG CTCCTGGAGT CCCTGGGAAT TCAGGGCCTA GAGGTTTTCC AGGGCCCAAA 
GGATCTAAAG GCAATAAAGG TCCAAAAGGC AAAAACAGTC CTCGTGGTGA ATCTGGAAAC 
CGAGGGTCTC CTGGTCCTGT TGGTGTTCCT GGGCCCAGAG GGGTCATCGG TCGAGAGGGT 
TTTGAGGGGG ATCCTGGACC CGATGGAGCT GCTGGGAAAG ATGGAGCTAA AGGAATGCCA 
GGTGATCTTG GACATGATGG GGATGTTGGG CTGCCTGGAA AACCGGGTCC TCAAGGAAAT 
GCAGGAGCAC CAGGTCTACC AGGAGTTCAA GGCTCTTTTG GGCCAAAGGG GGAAAGGGGC 
ATCCCTGGCC AATCTGGACC TCCAGGCAAA AGAGGACTGA ACGGAGGAAT GGGATTTCCT 
GGAAAACAAG GCGACCAAGG CTTCAAAGGA CAACCTGGTG ATGCTGGTGA ACAGGGATTT 
CCTGGAGTTC TTGGAATATT TGGACCACAG GGGCCTCCTG GAGACTTCGG TCCAGTGGGT 
GTGCAAGGAC CTAAGGGTCC TCAGGGTTTA ATGGGGATGC AAGGAGCCAT CGGACCAGTT 
GGGATCATTG GGCCGAGTGG TAACCCAGGA CCGCAAGGAG ACAAAGGAAA CAAAGGGGAA 
ATGGGTGTTC AGGGACCCAG GGGGCCTCCA GGTCCTCGTG GACCACCGGG ACCTCCAGGG 
CTTCCTGCTG TAGCCTTTTC TCATGAAAAT GAGGCCCTTG GTGCTGCTTT ACATGTTTTT 
GATTCCCGCT CCGCAATAAG ATCTGAAATG TATCAGGATA CTGACCTTCC GCTGCTGGAT 
CAAGGAAGCG AGATTTTTAA GACTCTTCAC TACCTCAGCA TCCTGATCCA CAGTATCAAA 
AACCCGCTGG GAACGCAGGA GAATCCGGCC AGAATGTGCA GGGATCTGCT TGAATGCGAG 
CACCGGTTGA ATGATGGCAC TTATTGGATA GATCCTAACC TGGGCTGCTC CTCTGACAAC 
ATTGAAGTGA CCTGTAGCTT CACCAATGGA GGTCAAACCT GCCTTAAGCC AGTCACTGCA 
TCCAAGCTGG AGATCGGAGT CAGTCTGATC CAGATGAACT TCATCCACCT CCTGAGCTCT 
GAGGCTGTGC AGATCATCAC CGTTCACTGT CTGAACATCT CCGTTTGGGC ATCGGAAGAC 
TCCAAAACAC CTTCCTCCAG CATGGTATAC TTTAAAGCCT GGGATGGACA GATAATCGAG 
GCTGGAGGAT TCATCGAGCC TGATTTGCTA AAGGATGAGT GCTGGATCAC AGATGGTAGA 
TGGCATCAAA CACAGTTCAT TTTCCGGACG CAGGACCCAA ACTTACTACC CATTGTGGAG 
ATCTACAACT TGCCCAGCAC TAAACCTGGC TCACATTACC ACCTGGAGGT GGGCCCTGTG 
TGCTTTTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331876.1
CDS	106..2757
Translation	MKGEPGMTGNPGMLGERGPVGFVGPFGEMGLAGEKGDRGETGQPGPPGEKGAMGHPGAPGERGLSGPAGAPGPHGSRGLSGTRGPKGTRGARGPDGPVGEKGMMGMKGPEGPPGKQGLSGQMGKIGETGEAGPTGFTGVQGPTGPPGAKGILGEPGPQGSPGVLGPLGEIGAIGLPGKAGDQGLPGEPGEKGAVGSPGNIGEQGLIGPRGDPGVDGEAGPSGPDGAKGEQGDVGLEGESGEKGVIGFKGTEGRTGDPGLIGVKGPEGKIGKTGERGKPGEKGSKGHQGQLGEMGALGEQGDTGFIGPKGSRGTTGFMGATGKMGQHGEPGLVGYEGHQGPQGSLGSPGPKGEKGEQGDDGKVEGPPGPSGDIGPVGNRGDRGEPGDPGYPGLEGVQGERGKEGAPGVPGNSGPRGFPGPKGSKGNKGPKGKNSPRGESGNRGSPGPVGVPGPRGVIGREGFEGDPGPDGAAGKDGAKGMPGDLGHDGDVGLPGKPGPQGNAGAPGLPGVQGSFGPKGERGIPGQSGPPGKRGLNGGMGFPGKQGDQGFKGQPGDAGEQGFPGVLGIFGPQGPPGDFGPVGVQGPKGPQGLMGMQGAIGPVGIIGPSGNPGPQGDKGNKGEMGVQGPRGPPGPRGPPGPPGLPAVAFSHENEALGAALHVFDSRSAIRSEMYQDTDLPLLDQGSEIFKTLHYLSILIHSIKNPLGTQENPARMCRDLLECEHRLNDGTYWIDPNLGCSSDNIEVTCSFTNGGQTCLKPVTASKLEIGVSLIQMNFIHLLSSEAVQIITVHCLNISVWASEDSKTPSSSMVYFKAWDGQIIEAGGFIEPDLLKDECWITDGRWHQTQFIFRTQDPNLLPIVEIYNLPSTKPGSHYHLEVGPVCFL

Target ORF information:

RefSeq Version	XM_021476201.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XXVII, alpha 1b (col27a1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476201.1

ORF Insert Sequence:

ATGAAGGGAG AGCCTGGAAT GACTGGAAAT CCTGGGATGC TTGGTGAGAG GGGTCCTGTT 
GGTTTCGTCG GGCCTTTTGG TGAGATGGGA CTTGCTGGAG AAAAGGGAGA TCGTGGTGAG 
ACGGGTCAGC CTGGGCCACC TGGAGAAAAG GGAGCCATGG GTCACCCAGG GGCTCCAGGA 
GAGAGAGGCC TGTCTGGACC AGCAGGTGCT CCAGGGCCTC ATGGATCTAG GGGCCTCAGT 
GGCACCAGGG GGCCAAAAGG CACAAGGGGT GCAAGGGGAC CAGATGGACC AGTTGGAGAG 
AAAGGAATGA TGGGAATGAA GGGTCCTGAA GGTCCACCAG GAAAACAAGG TTTAAGCGGA 
CAAATGGGTA AAATTGGAGA AACTGGTGAA GCAGGACCTA CCGGATTTAC TGGTGTTCAA 
GGACCAACTG GACCTCCTGG AGCAAAAGGC ATCTTAGGAG AACCTGGTCC TCAAGGATCA 
CCGGGAGTGT TGGGTCCATT GGGTGAGATT GGAGCAATAG GTTTACCTGG GAAGGCTGGC 
GATCAGGGCT TACCGGGAGA ACCAGGAGAG AAGGGTGCAG TCGGGTCTCC TGGGAATATT 
GGAGAGCAAG GCCTGATTGG ACCCCGAGGT GACCCTGGAG TCGATGGAGA GGCTGGTCCA 
AGTGGTCCCG ATGGAGCCAA GGGTGAACAG GGGGATGTTG GACTGGAGGG AGAATCAGGC 
GAAAAAGGAG TCATCGGATT TAAAGGAACA GAGGGACGTA CTGGTGATCC TGGCTTAATA 
GGCGTCAAGG GCCCTGAAGG CAAAATAGGG AAAACTGGTG AGAGGGGAAA ACCTGGAGAG 
AAGGGAAGCA AAGGTCATCA AGGTCAACTT GGCGAGATGG GTGCTTTGGG TGAGCAAGGT 
GACACGGGTT TCATCGGGCC AAAAGGAAGC AGAGGAACTA CTGGATTTAT GGGTGCGACG 
GGAAAAATGG GTCAGCACGG AGAGCCAGGT TTGGTGGGAT ATGAAGGTCA TCAAGGGCCA 
CAAGGTTCAT TGGGATCCCC GGGACCAAAA GGTGAAAAGG GAGAGCAAGG CGATGACGGG 
AAGGTTGAAG GTCCACCTGG TCCTTCTGGT GACATCGGAC CTGTTGGCAA CAGAGGAGAC 
AGAGGAGAAC CTGGTGACCC TGGATACCCA GGCCTTGAAG GTGTTCAAGG AGAAAGAGGA 
AAAGAGGGGG CTCCTGGAGT CCCTGGGAAT TCAGGGCCTA GAGGTTTTCC AGGGCCCAAA 
GGATCTAAAG GCAATAAAGG TCCAAAAGGC AAAAACAGTC CTCGTGGTGA ATCTGGAAAC 
CGAGGGTCTC CTGGTCCTGT TGGTGTTCCT GGGCCCAGAG GGGTCATCGG TCGAGAGGGT 
TTTGAGGGGG ATCCTGGACC CGATGGAGCT GCTGGGAAAG ATGGAGCTAA AGGAATGCCA 
GGTGATCTTG GACATGATGG GGATGTTGGG CTGCCTGGAA AACCGGGTCC TCAAGGAAAT 
GCAGGAGCAC CAGGTCTACC AGGAGTTCAA GGCTCTTTTG GGCCAAAGGG GGAAAGGGGC 
ATCCCTGGCC AATCTGGACC TCCAGGCAAA AGAGGACTGA ACGGAGGAAT GGGATTTCCT 
GGAAAACAAG GCGACCAAGG CTTCAAAGGA CAACCTGGTG ATGCTGGTGA ACAGGGATTT 
CCTGGAGTTC TTGGAATATT TGGACCACAG GGGCCTCCTG GAGACTTCGG TCCAGTGGGT 
GTGCAAGGAC CTAAGGGTCC TCAGGGTTTA ATGGGGATGC AAGGAGCCAT CGGACCAGTT 
GGGATCATTG GGCCGAGTGG TAACCCAGGA CCGCAAGGAG ACAAAGGAAA CAAAGGGGAA 
ATGGGTGTTC AGGGACCCAG GGGGCCTCCA GGTCCTCGTG GACCACCGGG ACCTCCAGGG 
CTTCCTGCTG TAGCCTTTTC TCATGAAAAT GAGGCCCTTG GTGCTGCTTT ACATGTTTTT 
GATTCCCGCT CCGCAATAAG ATCTGAAATG TATCAGGATA CTGACCTTCC GCTGCTGGAT 
CAAGGAAGCG AGATTTTTAA GACTCTTCAC TACCTCAGCA TCCTGATCCA CAGTATCAAA 
AACCCGCTGG GAACGCAGGA GAATCCGGCC AGAATGTGCA GGGATCTGCT TGAATGCGAG 
CACCGGTTGA ATGATGGCAC TTATTGGATA GATCCTAACC TGGGCTGCTC CTCTGACAAC 
ATTGAAGTGA CCTGTAGCTT CACCAATGGA GGTCAAACCT GCCTTAAGCC AGTCACTGCA 
TCCAAGCTGG AGATCGGAGT CAGTCTGATC CAGATGAACT TCATCCACCT CCTGAGCTCT 
GAGGCTGTGC AGATCATCAC CGTTCACTGT CTGAACATCT CCGTTTGGGC ATCGGAAGAC 
TCCAAAACAC CTTCCTCCAG CATGGTATAC TTTAAAGCCT GGGATGGACA GATAATCGAG 
GCTGGAGGAT TCATCGAGCC TGATTTGCTA AAGGATGAGT GCTGGATCAC AGATGGTAGA 
TGGCATCAAA CACAGTTCAT TTTCCGGACG CAGGACCCAA ACTTACTACC CATTGTGGAG 
ATCTACAACT TGCCCAGCAC TAAACCTGGC TCACATTACC ACCTGGAGGT GGGCCCTGTG 
TGCTTTTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1B ( XM_009301947.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009300222.1 Danio rerio collagen, type XXVII, alpha 1b (col27a1b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa43244
Clone ID Related Accession (Same CDS sequence)	XM_009301947.1
Accession Version	XM_009301947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-1(XXVII) chain B isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG ACAATACCCC GAGCTCCAGA CTCAGAGCAG CTGGGGTCGG GGGGAGAGCA 
GTGTTTTTCT GCATGATTCT TTACTGTACA TGCTGTTTGA GGCTCGCACA AGCACAATCT 
GCAGATGTGG ACGTGCTGCA AAGACTGGGA TTGGTTGGGA AAAGACCACC TCAAGGCTTC 
ATCCCTATTA AATCAGGAGT CATCCTAACC ACAAGGGCGA GAATTGACGT CCCGGTGAGC 
TCCGTCATTC CTGTGTCACT GGGCTCGACG TTCTCTATCA TCCTCAGCGT ATGTTCCCAT 
CGCATAAACA GTGCTTTCCT TTTCACCATT GTGACAAAGA GGAAAAGACT GCACCTAGGG 
GTGCAGTTCA TCCCTGGACA GATATTAGTC TACCTAGGCC AAAACAGCTC TGTAAATTTT 
GATTACAATG TTCACAATGG CCAGTGGCAC AACCTGGCTT TGGAAATCCA AGGCCAGAAG 
GTTACTTTAT ATACTTCCTG TGGGAATACA AGCATACAGG CAAATCTGGA TTTTCAAACT 
GAGGAAACGC TGGATCCAGA GGGCTCCTTT CGCTTGGGAA AAATGAGCCA GAACTCAGTG 
CAGTTCGAAG GAGCCATCTG CCAGTTTGAC ATTCATCCCT CTGCTCAGGC AGCTCACAAT 
TATTGCAAGT ACATTAAAAA ACAATGCAGG GAGGCGGATA CTTATCGGCC AAACCTTCCT 
CCTCTTCTAC CACTATTACC CCTGGACCCT AATACATCTA CCATTCAAAC CCCCAAAGTT 
GTAACAGATT TTAACGAAAG ACATCTTTCA CTAACACGAG ATGAGATGAA TATTAATCAT 
GAAGGCCAAA CCACGGTGCC ACCAATGGTA ACACAGCCCA CATTACAGCT CCCCCTGCAG 
ACCACAGCGC AAACTACTGC CTCCATTAGG AACAGGACTT CACAAATATC TCCAAAGCCA 
ACTCAACAAA ACAGGAAGAA AGCAAAAAAA GAACGTAACA AGTTACATAT TTTAAAAGAG 
CACCAGATTT CTGTCACTGA CAGTCCAACA AGTCAACAAA AAAACCAAGT AGATATTCCA 
ACCACAACAA CACCTGAACT TTCTAGTACA TTCAGGGTGT CAGAAATGAC GACATTAGCA 
GCACAGAGAC CTCTGCCAAA GGAAACCTCC TTATTTGAAG CCAAAGCCAC CTTTTTTGAG 
TCTGTAACCC CTGCAGCTAT GGATGGTTTG CAAACGTTTG ACTTGGAACC GACCCAGTAT 
TCCTTCTTGG AGTTAACCGG ACTGAAAGGA GAACCCGGAC TACCGGGACC ACCAGGACCT 
CCGGGCCAGC CAGGATTACC GGGAAAGAGA GGTCCCCGGG GACCCTCTGG TCCGCATGGA 
AAACCAGGAC CCCCAGGGAC TCCTGGACCT AAGGGAAAAA AGGGAAATCC TGGAATCTCA 
CCTGGCAGAG CACCAAGTGG AGTAAAGGGC GACCCTGGAT TAGTAGGTCT GCCTGGACAA 
CCCGGTCAAC CCGGTCGTAA AGGACAGAAA GGCCATCCTG GTCCCTCTGG CCACCCTGGA 
GACCAAGGAG AAAGAGGACC GGATGGAAGT CCAGGTGCCA AAGGTTATCC TGGCAGGCAG 
GGCTTACCTG GGCCTATAGG AAATATGGGA CCAAAAGGCG TACGGGGCTT CATTGGAATC 
CCAGGCATCT TCGGTCTCCC TGGAGCTGAC GGAGAAAGAG GTTTACCTGG TGTTCCTGGG 
AAGAAGGGCA AGATGGGTAG GCCGGGCTTT CCAGGAGATT TTGGGGAGCG AGGACCACCA 
GGACCTGATG GAAACCCAGG GGAAATCGGC GCCCCGGGGC CTGTTGGCAT CCCTGGATTT 
ATTGGTGATA TGGGCCCTGT TGGAATGGTC GGTCCTCCTG GACTAATAGG ACCAAAGGGT 
GTCCCAGGAA ACCCAGGAGA GCCCGGACTG AAAGGTGATA AGGGTGAGCT CGGATTGCCA 
GGTGAACCCG GGGAACCTGG ATTTCAAGGT GACAAGGGTA TTCAGGGCTC ACCGGGTTTA 
CCAGGAGTTC AAGGAAAGCC AGGACCACAG GGGAAGATTG GAGATCGAGG TCCAGACGGT 
TTGCCCGGCC CACTTGGTCC TGAGGGTTTT CCAGGGGATA TTGGACCACC AGGACAGAAT 
GGTGTTGAGG GCCCAAAGGG GAATGCAGGA GTGAGAGGCA TTCCAGGCCC TGGAGGACTC 
CCTGGACTCG AGGGAGATCA GGGACCAGTG GGACCAGCAG GGGCTCCAGG ACTAGAGGGC 
AGACCTGGGA GGAAAGGTAT CTCTGGCAAT CCAGGGGAGG AAGGGATGAA GGGAGAGCCT 
GGAATGACTG GAAATCCTGG GATGCTTGGT GAGAGGGGTC CTGTTGGTTT CGTCGGGCCT 
TTTGGTGAGA TGGGACTTGC TGGAGAAAAG GGAGATCGTG GTGAGACGGG TCAGCCTGGG 
CCACCTGGAG AAAAGGGAGC CATGGGTCAC CCAGGGGCTC CAGGAGAGAG AGGCCTGTCT 
GGACCAGCAG GTGCTCCAGG GCCTCATGGA TCTAGGGGCC TCAGTGGCAC CAGGGGGCCA 
AAAGGCACAA GGGGTGCAAG GGGACCAGAT GGACCAGTTG GAGAGAAAGG AATGATGGGA 
ATGAAGGGTC CTGAAGGTCC ACCAGGAAAA CAAGGTTTAA GCGGACAAAT GGGTAAAATT 
GGAGAAACTG GTGAAGCAGG ACCTACCGGA TTTACTGGTG TTCAAGGACC AACTGGACCT 
CCTGGAGCAA AAGGCATCTT AGGAGAACCT GGTCCTCAAG GATCACCGGG AGTGTTGGGT 
CCATTGGGGT TTACCTGGGA AGGCTGGCGA TCAGGGCTTA CCGGGAGAAC CAGGAGAGAA 
GGGTGCAGTC GGGTCTCCTG GGAATATTGG AGAGCAAGGC CTGATTGGAC CCCGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300222.1
CDS	375..3314
Translation	MEPDNTPSSRLRAAGVGGRAVFFCMILYCTCCLRLAQAQSADVDVLQRLGLVGKRPPQGFIPIKSGVILTTRARIDVPVSSVIPVSLGSTFSIILSVCSHRINSAFLFTIVTKRKRLHLGVQFIPGQILVYLGQNSSVNFDYNVHNGQWHNLALEIQGQKVTLYTSCGNTSIQANLDFQTEETLDPEGSFRLGKMSQNSVQFEGAICQFDIHPSAQAAHNYCKYIKKQCREADTYRPNLPPLLPLLPLDPNTSTIQTPKVVTDFNERHLSLTRDEMNINHEGQTTVPPMVTQPTLQLPLQTTAQTTASIRNRTSQISPKPTQQNRKKAKKERNKLHILKEHQISVTDSPTSQQKNQVDIPTTTTPELSSTFRVSEMTTLAAQRPLPKETSLFEAKATFFESVTPAAMDGLQTFDLEPTQYSFLELTGLKGEPGLPGPPGPPGQPGLPGKRGPRGPSGPHGKPGPPGTPGPKGKKGNPGISPGRAPSGVKGDPGLVGLPGQPGQPGRKGQKGHPGPSGHPGDQGERGPDGSPGAKGYPGRQGLPGPIGNMGPKGVRGFIGIPGIFGLPGADGERGLPGVPGKKGKMGRPGFPGDFGERGPPGPDGNPGEIGAPGPVGIPGFIGDMGPVGMVGPPGLIGPKGVPGNPGEPGLKGDKGELGLPGEPGEPGFQGDKGIQGSPGLPGVQGKPGPQGKIGDRGPDGLPGPLGPEGFPGDIGPPGQNGVEGPKGNAGVRGIPGPGGLPGLEGDQGPVGPAGAPGLEGRPGRKGISGNPGEEGMKGEPGMTGNPGMLGERGPVGFVGPFGEMGLAGEKGDRGETGQPGPPGEKGAMGHPGAPGERGLSGPAGAPGPHGSRGLSGTRGPKGTRGARGPDGPVGEKGMMGMKGPEGPPGKQGLSGQMGKIGETGEAGPTGFTGVQGPTGPPGAKGILGEPGPQGSPGVLGPLGFTWEGWRSGLTGRTRREGCSRVSWEYWRARPDWTPR

Target ORF information:

RefSeq Version	XM_009301947.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XXVII, alpha 1b (col27a1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301947.1

ORF Insert Sequence:

ATGGAGCCGG ACAATACCCC GAGCTCCAGA CTCAGAGCAG CTGGGGTCGG GGGGAGAGCA 
GTGTTTTTCT GCATGATTCT TTACTGTACA TGCTGTTTGA GGCTCGCACA AGCACAATCT 
GCAGATGTGG ACGTGCTGCA AAGACTGGGA TTGGTTGGGA AAAGACCACC TCAAGGCTTC 
ATCCCTATTA AATCAGGAGT CATCCTAACC ACAAGGGCGA GAATTGACGT CCCGGTGAGC 
TCCGTCATTC CTGTGTCACT GGGCTCGACG TTCTCTATCA TCCTCAGCGT ATGTTCCCAT 
CGCATAAACA GTGCTTTCCT TTTCACCATT GTGACAAAGA GGAAAAGACT GCACCTAGGG 
GTGCAGTTCA TCCCTGGACA GATATTAGTC TACCTAGGCC AAAACAGCTC TGTAAATTTT 
GATTACAATG TTCACAATGG CCAGTGGCAC AACCTGGCTT TGGAAATCCA AGGCCAGAAG 
GTTACTTTAT ATACTTCCTG TGGGAATACA AGCATACAGG CAAATCTGGA TTTTCAAACT 
GAGGAAACGC TGGATCCAGA GGGCTCCTTT CGCTTGGGAA AAATGAGCCA GAACTCAGTG 
CAGTTCGAAG GAGCCATCTG CCAGTTTGAC ATTCATCCCT CTGCTCAGGC AGCTCACAAT 
TATTGCAAGT ACATTAAAAA ACAATGCAGG GAGGCGGATA CTTATCGGCC AAACCTTCCT 
CCTCTTCTAC CACTATTACC CCTGGACCCT AATACATCTA CCATTCAAAC CCCCAAAGTT 
GTAACAGATT TTAACGAAAG ACATCTTTCA CTAACACGAG ATGAGATGAA TATTAATCAT 
GAAGGCCAAA CCACGGTGCC ACCAATGGTA ACACAGCCCA CATTACAGCT CCCCCTGCAG 
ACCACAGCGC AAACTACTGC CTCCATTAGG AACAGGACTT CACAAATATC TCCAAAGCCA 
ACTCAACAAA ACAGGAAGAA AGCAAAAAAA GAACGTAACA AGTTACATAT TTTAAAAGAG 
CACCAGATTT CTGTCACTGA CAGTCCAACA AGTCAACAAA AAAACCAAGT AGATATTCCA 
ACCACAACAA CACCTGAACT TTCTAGTACA TTCAGGGTGT CAGAAATGAC GACATTAGCA 
GCACAGAGAC CTCTGCCAAA GGAAACCTCC TTATTTGAAG CCAAAGCCAC CTTTTTTGAG 
TCTGTAACCC CTGCAGCTAT GGATGGTTTG CAAACGTTTG ACTTGGAACC GACCCAGTAT 
TCCTTCTTGG AGTTAACCGG ACTGAAAGGA GAACCCGGAC TACCGGGACC ACCAGGACCT 
CCGGGCCAGC CAGGATTACC GGGAAAGAGA GGTCCCCGGG GACCCTCTGG TCCGCATGGA 
AAACCAGGAC CCCCAGGGAC TCCTGGACCT AAGGGAAAAA AGGGAAATCC TGGAATCTCA 
CCTGGCAGAG CACCAAGTGG AGTAAAGGGC GACCCTGGAT TAGTAGGTCT GCCTGGACAA 
CCCGGTCAAC CCGGTCGTAA AGGACAGAAA GGCCATCCTG GTCCCTCTGG CCACCCTGGA 
GACCAAGGAG AAAGAGGACC GGATGGAAGT CCAGGTGCCA AAGGTTATCC TGGCAGGCAG 
GGCTTACCTG GGCCTATAGG AAATATGGGA CCAAAAGGCG TACGGGGCTT CATTGGAATC 
CCAGGCATCT TCGGTCTCCC TGGAGCTGAC GGAGAAAGAG GTTTACCTGG TGTTCCTGGG 
AAGAAGGGCA AGATGGGTAG GCCGGGCTTT CCAGGAGATT TTGGGGAGCG AGGACCACCA 
GGACCTGATG GAAACCCAGG GGAAATCGGC GCCCCGGGGC CTGTTGGCAT CCCTGGATTT 
ATTGGTGATA TGGGCCCTGT TGGAATGGTC GGTCCTCCTG GACTAATAGG ACCAAAGGGT 
GTCCCAGGAA ACCCAGGAGA GCCCGGACTG AAAGGTGATA AGGGTGAGCT CGGATTGCCA 
GGTGAACCCG GGGAACCTGG ATTTCAAGGT GACAAGGGTA TTCAGGGCTC ACCGGGTTTA 
CCAGGAGTTC AAGGAAAGCC AGGACCACAG GGGAAGATTG GAGATCGAGG TCCAGACGGT 
TTGCCCGGCC CACTTGGTCC TGAGGGTTTT CCAGGGGATA TTGGACCACC AGGACAGAAT 
GGTGTTGAGG GCCCAAAGGG GAATGCAGGA GTGAGAGGCA TTCCAGGCCC TGGAGGACTC 
CCTGGACTCG AGGGAGATCA GGGACCAGTG GGACCAGCAG GGGCTCCAGG ACTAGAGGGC 
AGACCTGGGA GGAAAGGTAT CTCTGGCAAT CCAGGGGAGG AAGGGATGAA GGGAGAGCCT 
GGAATGACTG GAAATCCTGG GATGCTTGGT GAGAGGGGTC CTGTTGGTTT CGTCGGGCCT 
TTTGGTGAGA TGGGACTTGC TGGAGAAAAG GGAGATCGTG GTGAGACGGG TCAGCCTGGG 
CCACCTGGAG AAAAGGGAGC CATGGGTCAC CCAGGGGCTC CAGGAGAGAG AGGCCTGTCT 
GGACCAGCAG GTGCTCCAGG GCCTCATGGA TCTAGGGGCC TCAGTGGCAC CAGGGGGCCA 
AAAGGCACAA GGGGTGCAAG GGGACCAGAT GGACCAGTTG GAGAGAAAGG AATGATGGGA 
ATGAAGGGTC CTGAAGGTCC ACCAGGAAAA CAAGGTTTAA GCGGACAAAT GGGTAAAATT 
GGAGAAACTG GTGAAGCAGG ACCTACCGGA TTTACTGGTG TTCAAGGACC AACTGGACCT 
CCTGGAGCAA AAGGCATCTT AGGAGAACCT GGTCCTCAAG GATCACCGGG AGTGTTGGGT 
CCATTGGGGT TTACCTGGGA AGGCTGGCGA TCAGGGCTTA CCGGGAGAAC CAGGAGAGAA 
GGGTGCAGTC GGGTCTCCTG GGAATATTGG AGAGCAAGGC CTGATTGGAC CCCGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1B ( XM_017356118.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017211607.1 Danio rerio collagen, type XXVII, alpha 1b (col27a1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa59099
Clone ID Related Accession (Same CDS sequence)	XM_017356118.1 , XM_017356118.2
Accession Version	XM_017356118.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-1(XXVII) chain B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG ACAATACCCC GAGCTCCAGA CTCAGAGCAG CTGGGGTCGG GGGGAGAGCA 
GTGTTTTTCT GCATGATTCT TTACTGTACA TGCTGTTTGA GGCTCGCACA AGCACAATCT 
GCAGATGTGG ACGTGCTGCA AAGACTGGGA TTGGTTGGGA AAAGACCACC TCAAGGCTTC 
ATCCCTATTA AATCAGGAGT CATCCTAACC ACAAGGGCGA GAATTGACGT CCCGGTGAGC 
TCCGTCATTC CTGTGTCACT GGGCTCGACG TTCTCTATCA TCCTCAGCGT ATGTTCCCAT 
CGCATAAACA GTGCTTTCCT TTTCACCATT GTGACAAAGA GGAAAAGACT GCACCTAGGG 
GTGCAGTTCA TCCCTGGACA GATATTAGTC TACCTAGGCC AAAACAGCTC TGTAAATTTT 
GATTACAATG TTCACAATGG CCAGTGGCAC AACCTGGCTT TGGAAATCCA AGGCCAGAAG 
GTTACTTTAT ATACTTCCTG TGGGAATACA AGCATACAGG CAAATCTGGA TTTTCAAACT 
GAGGAAACGC TGGATCCAGA GGGCTCCTTT CGCTTGGGAA AAATGAGCCA GAACTCAGTG 
CAGTTCGAAG GAGCCATCTG CCAGTTTGAC ATTCATCCCT CTGCTCAGGC AGCTCACAAT 
TATTGCAAGT ACATTAAAAA ACAATGCAGG GAGGCGGATA CTTATCGGCC AAACCTTCCT 
CCTCTTCTAC CACTATTACC CCTGGACCCT AATACATCTA CCATTCAAAC CCCCAAAGTT 
GTAACAGATT TTAACGAAAG ACATCTTTCA CTAACACGAG ATGAGATGAA TATTAATCAT 
GAAGGCCAAA CCACGGTGCC ACCAATGGTA ACACAGCCCA CATTACAGCT CCCCCTGCAG 
ACCACAGCGC AAACTACTGC CTCCATTAGG AACAGGACTT CACAAATATC TCCAAAGCCA 
ACTCAACAAA ACAGGAAGAA AGCAAAAAAA GAACGTAACA AGTTACATAT TTTAAAAGAG 
CACCAGATTT CTGTCACTGA CAGTCCAACA AGTCAACAAA AAAACCAAGT AGATATTCCA 
ACCACAACAA CACCTGAACT TTCTAGTACA TTCAGGGTGT CAGAAATGAC GACATTAGCA 
GCACAGAGAC CTCTGCCAAA GGAAACCTCC TTATTTGAAG CCAAAGCCAC CTTTTTTGAG 
TCTGTAACCC CTGCAGCTAT GGATGGTTTG CAAACGTTTG ACTTGGAACC GACCCAGTAT 
TCCTTCTTGG AGTTAACCGG ACTGAAAGGA GAACCCGGAC TACCGGGACC ACCAGGACCT 
CCGGGCCAGC CAGGATTACC GGGAAAGAGA GGTCCCCGGG GACCCTCTGG TCCGCATGGA 
AAACCAGGAC CCCCAGGGAC TCCTGGACCT AAGGGAAAAA AGGGAAATCC TGGAATCTCA 
CCTGGCAGAG CACCAAGTGG AGTAAAGGGC GACCCTGGAT TAGTAGGTCT GCCTGGACAA 
CCCGGTCAAC CCGGTCGTAA AGGACAGAAA GGCCATCCTG GTCCCTCTGG CCACCCTGGA 
GACCAAGGAG AAAGAGGACC GGATGGAAGT CCAGGTGCCA AAGGTTATCC TGGCAGGCAG 
GGCTTACCTG GGCCTATAGG AAATATGGGA CCAAAAGGCG TACGGGGCTT CATTGGAATC 
CCAGGCATCT TCGGTCTCCC TGGAGCTGAC GGAGAAAGAG GTTTACCTGG TGTTCCTGGG 
AAGAAGGGCA AGATGGGTAG GCCGGGCTTT CCAGGAGATT TTGGGGAGCG AGGACCACCA 
GGACCTGATG GAAACCCAGG GGAAATCGGC GCCCCGGGGC CTGTTGGCAT CCCTGGATTT 
ATTGGTGATA TGGGCCCTGT TGGAATGGTC GGTCCTCCTG GACTAATAGG ACCAAAGGGT 
GTCCCAGGAA ACCCAGGAGA GCCCGGACTG AAAGGTGATA AGGGTGAGCT CGGATTGCCA 
GGTGAACCCG GGGAACCTGG ATTTCAAGGT GACAAGGGTA TTCAGGGCTC ACCGGGTTTA 
CCAGGAGTTC AAGGAAAGCC AGGACCACAG GGGAAGATTG GAGATCGAGG TCCAGACGGT 
TTGCCCGGCC CACTTGGTCC TGAGGGTTTT CCAGGGGATA TTGGACCACC AGGACAGAAT 
GGTGTTGAGG GCCCAAAGGG GAATGCAGGA GTGAGAGGCA TTCCAGGCCC TGGAGGACTC 
CCTGGACTCG AGGGAGATCA GGGACCAGTG GGACCAGCAG GGGCTCCAGG ACTAGAGGGC 
AGACCTGGGA GGAAAGGTAT CTCTGGCAAT CCAGGGGAGG AAGGGATGAA GGGAGAGCCT 
GGAATGACTG GAAATCCTGG GATGCTTGGT GAGAGGGGTC CTGTTGGTTT CGTCGGGCCT 
TTTGGTGAGA TGGGACTTGC TGGAGAAAAG GGAGATCGTG GTGAGACGGG TCAGCCTGGG 
CCACCTGGAG AAAAGGGAGC CATGGGTCAC CCAGGGGCTC CAGGAGAGAG AGGCCTGTCT 
GGACCAGCAG GTGCTCCAGG GCCTCATGGA TCTAGGGGCC TCAGTGGCAC CAGGGGGCCA 
AAAGGCACAA GGGGTGCAAG GGGACCAGAT GGACCAGTTG GAGAGAAAGG AATGATGGGA 
ATGAAGGGTC CTGAAGGTCC ACCAGGAAAA CAAGGTTTAA GCGGACAAAT GGGTAAAATT 
GGAGAAACTG GTGAAGCAGG ACCTACCGGA TTTACTGGTG TTCAAGGACC AACTGGACCT 
CCTGGAGCAA AAGGCATCTT AGGAGAACCT GGTCCTCAAG GATCACCGGG AGTGTTGGGT 
CCATTGGGTG AGATTGGAGC AATAGGTTTA CCTGGGAAGG CTGGCGATCA GGGCTTACCG 
GGAGAACCAG GAGAGAAGGG TGCAGTCGGG TCTCCTGGGA ATATTGGAGA GCAAGGCCTG 
ATTGGACCCC GAGGTGACCC TGGAGTCGAT GGAGAGGCTG GTCCAAGTGG TCCCGATGGA 
GCCAAGGGTG AACAGGGGGA TGTTGGACTG GAGGGAGAAT CAGGCGAAAA AGGAGTCATC 
GGATTTAAAG GAACAGAGGG ACGTACTGGT GATCCTGGCT TAATAGGCGT CAAGGGCCCT 
GAAGGCAAAA TAGGGAAAAC TGGTGAGAGG GGAAAACCTG GAGAGAAGGG AAGCAAAGGT 
CATCAAGGTC AACTTGGCGA GATGGGTGCT TTGGGTGAGC AAGGTGACAC GGGTTTCATC 
GGGCCAAAAG GAAGCAGAGG AACTACTGGA TTTATGGGTG CGACGGGAAA AATGGGTCAG 
CACGGAGAGC CAGGTTTGGT GGGATATGAA GGTCATCAAG GGCCACAAGG TTCATTGGGA 
TCCCCGGGAC CAAAAGGTGA AAAGGGAGAG CAAGGCGATG ACGGGAAGGT TGAAGGTCCA 
CCTGGTCCTT CTGGTGACAT CGGACCTGTT GGCAACAGAG GAGACAGAGG AGAACCTGGT 
GACCCTGGAT ACCCAGGCCT TGAAGGTGTT CAAGGAGAAA GAGGAAAAGA GGGGGCTCCT 
GGAGTCCCTG GGAATTCAGG GCCTAGAGGT TTTCCAGGGC CCAAAGGATC TAAAGGCAAT 
AAAGGTCCAA AAGGCAAAAA CAGTCCTCGT GGTGAATCTG GAAACCGAGG GTCTCCTGGT 
CCTGTTGGTG TTCCTGGGCC CAGAGGGGTC ATCGGTCGAG AGGGTTTTGA GGGGGATCCT 
GGACCCGATG GAGCTGCTGG GAAAGATGGA GCTAAAGGAA TGCCAGTAAG ACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211607.1
CDS	375..4151
Translation	MEPDNTPSSRLRAAGVGGRAVFFCMILYCTCCLRLAQAQSADVDVLQRLGLVGKRPPQGFIPIKSGVILTTRARIDVPVSSVIPVSLGSTFSIILSVCSHRINSAFLFTIVTKRKRLHLGVQFIPGQILVYLGQNSSVNFDYNVHNGQWHNLALEIQGQKVTLYTSCGNTSIQANLDFQTEETLDPEGSFRLGKMSQNSVQFEGAICQFDIHPSAQAAHNYCKYIKKQCREADTYRPNLPPLLPLLPLDPNTSTIQTPKVVTDFNERHLSLTRDEMNINHEGQTTVPPMVTQPTLQLPLQTTAQTTASIRNRTSQISPKPTQQNRKKAKKERNKLHILKEHQISVTDSPTSQQKNQVDIPTTTTPELSSTFRVSEMTTLAAQRPLPKETSLFEAKATFFESVTPAAMDGLQTFDLEPTQYSFLELTGLKGEPGLPGPPGPPGQPGLPGKRGPRGPSGPHGKPGPPGTPGPKGKKGNPGISPGRAPSGVKGDPGLVGLPGQPGQPGRKGQKGHPGPSGHPGDQGERGPDGSPGAKGYPGRQGLPGPIGNMGPKGVRGFIGIPGIFGLPGADGERGLPGVPGKKGKMGRPGFPGDFGERGPPGPDGNPGEIGAPGPVGIPGFIGDMGPVGMVGPPGLIGPKGVPGNPGEPGLKGDKGELGLPGEPGEPGFQGDKGIQGSPGLPGVQGKPGPQGKIGDRGPDGLPGPLGPEGFPGDIGPPGQNGVEGPKGNAGVRGIPGPGGLPGLEGDQGPVGPAGAPGLEGRPGRKGISGNPGEEGMKGEPGMTGNPGMLGERGPVGFVGPFGEMGLAGEKGDRGETGQPGPPGEKGAMGHPGAPGERGLSGPAGAPGPHGSRGLSGTRGPKGTRGARGPDGPVGEKGMMGMKGPEGPPGKQGLSGQMGKIGETGEAGPTGFTGVQGPTGPPGAKGILGEPGPQGSPGVLGPLGEIGAIGLPGKAGDQGLPGEPGEKGAVGSPGNIGEQGLIGPRGDPGVDGEAGPSGPDGAKGEQGDVGLEGESGEKGVIGFKGTEGRTGDPGLIGVKGPEGKIGKTGERGKPGEKGSKGHQGQLGEMGALGEQGDTGFIGPKGSRGTTGFMGATGKMGQHGEPGLVGYEGHQGPQGSLGSPGPKGEKGEQGDDGKVEGPPGPSGDIGPVGNRGDRGEPGDPGYPGLEGVQGERGKEGAPGVPGNSGPRGFPGPKGSKGNKGPKGKNSPRGESGNRGSPGPVGVPGPRGVIGREGFEGDPGPDGAAGKDGAKGMPVRQ

Target ORF information:

RefSeq Version	XM_017356118.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XXVII, alpha 1b (col27a1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356118.1

ORF Insert Sequence:

ATGGAGCCGG ACAATACCCC GAGCTCCAGA CTCAGAGCAG CTGGGGTCGG GGGGAGAGCA 
GTGTTTTTCT GCATGATTCT TTACTGTACA TGCTGTTTGA GGCTCGCACA AGCACAATCT 
GCAGATGTGG ACGTGCTGCA AAGACTGGGA TTGGTTGGGA AAAGACCACC TCAAGGCTTC 
ATCCCTATTA AATCAGGAGT CATCCTAACC ACAAGGGCGA GAATTGACGT CCCGGTGAGC 
TCCGTCATTC CTGTGTCACT GGGCTCGACG TTCTCTATCA TCCTCAGCGT ATGTTCCCAT 
CGCATAAACA GTGCTTTCCT TTTCACCATT GTGACAAAGA GGAAAAGACT GCACCTAGGG 
GTGCAGTTCA TCCCTGGACA GATATTAGTC TACCTAGGCC AAAACAGCTC TGTAAATTTT 
GATTACAATG TTCACAATGG CCAGTGGCAC AACCTGGCTT TGGAAATCCA AGGCCAGAAG 
GTTACTTTAT ATACTTCCTG TGGGAATACA AGCATACAGG CAAATCTGGA TTTTCAAACT 
GAGGAAACGC TGGATCCAGA GGGCTCCTTT CGCTTGGGAA AAATGAGCCA GAACTCAGTG 
CAGTTCGAAG GAGCCATCTG CCAGTTTGAC ATTCATCCCT CTGCTCAGGC AGCTCACAAT 
TATTGCAAGT ACATTAAAAA ACAATGCAGG GAGGCGGATA CTTATCGGCC AAACCTTCCT 
CCTCTTCTAC CACTATTACC CCTGGACCCT AATACATCTA CCATTCAAAC CCCCAAAGTT 
GTAACAGATT TTAACGAAAG ACATCTTTCA CTAACACGAG ATGAGATGAA TATTAATCAT 
GAAGGCCAAA CCACGGTGCC ACCAATGGTA ACACAGCCCA CATTACAGCT CCCCCTGCAG 
ACCACAGCGC AAACTACTGC CTCCATTAGG AACAGGACTT CACAAATATC TCCAAAGCCA 
ACTCAACAAA ACAGGAAGAA AGCAAAAAAA GAACGTAACA AGTTACATAT TTTAAAAGAG 
CACCAGATTT CTGTCACTGA CAGTCCAACA AGTCAACAAA AAAACCAAGT AGATATTCCA 
ACCACAACAA CACCTGAACT TTCTAGTACA TTCAGGGTGT CAGAAATGAC GACATTAGCA 
GCACAGAGAC CTCTGCCAAA GGAAACCTCC TTATTTGAAG CCAAAGCCAC CTTTTTTGAG 
TCTGTAACCC CTGCAGCTAT GGATGGTTTG CAAACGTTTG ACTTGGAACC GACCCAGTAT 
TCCTTCTTGG AGTTAACCGG ACTGAAAGGA GAACCCGGAC TACCGGGACC ACCAGGACCT 
CCGGGCCAGC CAGGATTACC GGGAAAGAGA GGTCCCCGGG GACCCTCTGG TCCGCATGGA 
AAACCAGGAC CCCCAGGGAC TCCTGGACCT AAGGGAAAAA AGGGAAATCC TGGAATCTCA 
CCTGGCAGAG CACCAAGTGG AGTAAAGGGC GACCCTGGAT TAGTAGGTCT GCCTGGACAA 
CCCGGTCAAC CCGGTCGTAA AGGACAGAAA GGCCATCCTG GTCCCTCTGG CCACCCTGGA 
GACCAAGGAG AAAGAGGACC GGATGGAAGT CCAGGTGCCA AAGGTTATCC TGGCAGGCAG 
GGCTTACCTG GGCCTATAGG AAATATGGGA CCAAAAGGCG TACGGGGCTT CATTGGAATC 
CCAGGCATCT TCGGTCTCCC TGGAGCTGAC GGAGAAAGAG GTTTACCTGG TGTTCCTGGG 
AAGAAGGGCA AGATGGGTAG GCCGGGCTTT CCAGGAGATT TTGGGGAGCG AGGACCACCA 
GGACCTGATG GAAACCCAGG GGAAATCGGC GCCCCGGGGC CTGTTGGCAT CCCTGGATTT 
ATTGGTGATA TGGGCCCTGT TGGAATGGTC GGTCCTCCTG GACTAATAGG ACCAAAGGGT 
GTCCCAGGAA ACCCAGGAGA GCCCGGACTG AAAGGTGATA AGGGTGAGCT CGGATTGCCA 
GGTGAACCCG GGGAACCTGG ATTTCAAGGT GACAAGGGTA TTCAGGGCTC ACCGGGTTTA 
CCAGGAGTTC AAGGAAAGCC AGGACCACAG GGGAAGATTG GAGATCGAGG TCCAGACGGT 
TTGCCCGGCC CACTTGGTCC TGAGGGTTTT CCAGGGGATA TTGGACCACC AGGACAGAAT 
GGTGTTGAGG GCCCAAAGGG GAATGCAGGA GTGAGAGGCA TTCCAGGCCC TGGAGGACTC 
CCTGGACTCG AGGGAGATCA GGGACCAGTG GGACCAGCAG GGGCTCCAGG ACTAGAGGGC 
AGACCTGGGA GGAAAGGTAT CTCTGGCAAT CCAGGGGAGG AAGGGATGAA GGGAGAGCCT 
GGAATGACTG GAAATCCTGG GATGCTTGGT GAGAGGGGTC CTGTTGGTTT CGTCGGGCCT 
TTTGGTGAGA TGGGACTTGC TGGAGAAAAG GGAGATCGTG GTGAGACGGG TCAGCCTGGG 
CCACCTGGAG AAAAGGGAGC CATGGGTCAC CCAGGGGCTC CAGGAGAGAG AGGCCTGTCT 
GGACCAGCAG GTGCTCCAGG GCCTCATGGA TCTAGGGGCC TCAGTGGCAC CAGGGGGCCA 
AAAGGCACAA GGGGTGCAAG GGGACCAGAT GGACCAGTTG GAGAGAAAGG AATGATGGGA 
ATGAAGGGTC CTGAAGGTCC ACCAGGAAAA CAAGGTTTAA GCGGACAAAT GGGTAAAATT 
GGAGAAACTG GTGAAGCAGG ACCTACCGGA TTTACTGGTG TTCAAGGACC AACTGGACCT 
CCTGGAGCAA AAGGCATCTT AGGAGAACCT GGTCCTCAAG GATCACCGGG AGTGTTGGGT 
CCATTGGGTG AGATTGGAGC AATAGGTTTA CCTGGGAAGG CTGGCGATCA GGGCTTACCG 
GGAGAACCAG GAGAGAAGGG TGCAGTCGGG TCTCCTGGGA ATATTGGAGA GCAAGGCCTG 
ATTGGACCCC GAGGTGACCC TGGAGTCGAT GGAGAGGCTG GTCCAAGTGG TCCCGATGGA 
GCCAAGGGTG AACAGGGGGA TGTTGGACTG GAGGGAGAAT CAGGCGAAAA AGGAGTCATC 
GGATTTAAAG GAACAGAGGG ACGTACTGGT GATCCTGGCT TAATAGGCGT CAAGGGCCCT 
GAAGGCAAAA TAGGGAAAAC TGGTGAGAGG GGAAAACCTG GAGAGAAGGG AAGCAAAGGT 
CATCAAGGTC AACTTGGCGA GATGGGTGCT TTGGGTGAGC AAGGTGACAC GGGTTTCATC 
GGGCCAAAAG GAAGCAGAGG AACTACTGGA TTTATGGGTG CGACGGGAAA AATGGGTCAG 
CACGGAGAGC CAGGTTTGGT GGGATATGAA GGTCATCAAG GGCCACAAGG TTCATTGGGA 
TCCCCGGGAC CAAAAGGTGA AAAGGGAGAG CAAGGCGATG ACGGGAAGGT TGAAGGTCCA 
CCTGGTCCTT CTGGTGACAT CGGACCTGTT GGCAACAGAG GAGACAGAGG AGAACCTGGT 
GACCCTGGAT ACCCAGGCCT TGAAGGTGTT CAAGGAGAAA GAGGAAAAGA GGGGGCTCCT 
GGAGTCCCTG GGAATTCAGG GCCTAGAGGT TTTCCAGGGC CCAAAGGATC TAAAGGCAAT 
AAAGGTCCAA AAGGCAAAAA CAGTCCTCGT GGTGAATCTG GAAACCGAGG GTCTCCTGGT 
CCTGTTGGTG TTCCTGGGCC CAGAGGGGTC ATCGGTCGAG AGGGTTTTGA GGGGGATCCT 
GGACCCGATG GAGCTGCTGG GAAAGATGGA GCTAAAGGAA TGCCAGTAAG ACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1B ( XM_017356118.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017211607.1 Danio rerio collagen, type XXVII, alpha 1b (col27a1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa59099
Clone ID Related Accession (Same CDS sequence)	XM_017356118.1 , XM_017356118.2
Accession Version	XM_017356118.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(XXVII) chain B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356118.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG ACAATACCCC GAGCTCCAGA CTCAGAGCAG CTGGGGTCGG GGGGAGAGCA 
GTGTTTTTCT GCATGATTCT TTACTGTACA TGCTGTTTGA GGCTCGCACA AGCACAATCT 
GCAGATGTGG ACGTGCTGCA AAGACTGGGA TTGGTTGGGA AAAGACCACC TCAAGGCTTC 
ATCCCTATTA AATCAGGAGT CATCCTAACC ACAAGGGCGA GAATTGACGT CCCGGTGAGC 
TCCGTCATTC CTGTGTCACT GGGCTCGACG TTCTCTATCA TCCTCAGCGT ATGTTCCCAT 
CGCATAAACA GTGCTTTCCT TTTCACCATT GTGACAAAGA GGAAAAGACT GCACCTAGGG 
GTGCAGTTCA TCCCTGGACA GATATTAGTC TACCTAGGCC AAAACAGCTC TGTAAATTTT 
GATTACAATG TTCACAATGG CCAGTGGCAC AACCTGGCTT TGGAAATCCA AGGCCAGAAG 
GTTACTTTAT ATACTTCCTG TGGGAATACA AGCATACAGG CAAATCTGGA TTTTCAAACT 
GAGGAAACGC TGGATCCAGA GGGCTCCTTT CGCTTGGGAA AAATGAGCCA GAACTCAGTG 
CAGTTCGAAG GAGCCATCTG CCAGTTTGAC ATTCATCCCT CTGCTCAGGC AGCTCACAAT 
TATTGCAAGT ACATTAAAAA ACAATGCAGG GAGGCGGATA CTTATCGGCC AAACCTTCCT 
CCTCTTCTAC CACTATTACC CCTGGACCCT AATACATCTA CCATTCAAAC CCCCAAAGTT 
GTAACAGATT TTAACGAAAG ACATCTTTCA CTAACACGAG ATGAGATGAA TATTAATCAT 
GAAGGCCAAA CCACGGTGCC ACCAATGGTA ACACAGCCCA CATTACAGCT CCCCCTGCAG 
ACCACAGCGC AAACTACTGC CTCCATTAGG AACAGGACTT CACAAATATC TCCAAAGCCA 
ACTCAACAAA ACAGGAAGAA AGCAAAAAAA GAACGTAACA AGTTACATAT TTTAAAAGAG 
CACCAGATTT CTGTCACTGA CAGTCCAACA AGTCAACAAA AAAACCAAGT AGATATTCCA 
ACCACAACAA CACCTGAACT TTCTAGTACA TTCAGGGTGT CAGAAATGAC GACATTAGCA 
GCACAGAGAC CTCTGCCAAA GGAAACCTCC TTATTTGAAG CCAAAGCCAC CTTTTTTGAG 
TCTGTAACCC CTGCAGCTAT GGATGGTTTG CAAACGTTTG ACTTGGAACC GACCCAGTAT 
TCCTTCTTGG AGTTAACCGG ACTGAAAGGA GAACCCGGAC TACCGGGACC ACCAGGACCT 
CCGGGCCAGC CAGGATTACC GGGAAAGAGA GGTCCCCGGG GACCCTCTGG TCCGCATGGA 
AAACCAGGAC CCCCAGGGAC TCCTGGACCT AAGGGAAAAA AGGGAAATCC TGGAATCTCA 
CCTGGCAGAG CACCAAGTGG AGTAAAGGGC GACCCTGGAT TAGTAGGTCT GCCTGGACAA 
CCCGGTCAAC CCGGTCGTAA AGGACAGAAA GGCCATCCTG GTCCCTCTGG CCACCCTGGA 
GACCAAGGAG AAAGAGGACC GGATGGAAGT CCAGGTGCCA AAGGTTATCC TGGCAGGCAG 
GGCTTACCTG GGCCTATAGG AAATATGGGA CCAAAAGGCG TACGGGGCTT CATTGGAATC 
CCAGGCATCT TCGGTCTCCC TGGAGCTGAC GGAGAAAGAG GTTTACCTGG TGTTCCTGGG 
AAGAAGGGCA AGATGGGTAG GCCGGGCTTT CCAGGAGATT TTGGGGAGCG AGGACCACCA 
GGACCTGATG GAAACCCAGG GGAAATCGGC GCCCCGGGGC CTGTTGGCAT CCCTGGATTT 
ATTGGTGATA TGGGCCCTGT TGGAATGGTC GGTCCTCCTG GACTAATAGG ACCAAAGGGT 
GTCCCAGGAA ACCCAGGAGA GCCCGGACTG AAAGGTGATA AGGGTGAGCT CGGATTGCCA 
GGTGAACCCG GGGAACCTGG ATTTCAAGGT GACAAGGGTA TTCAGGGCTC ACCGGGTTTA 
CCAGGAGTTC AAGGAAAGCC AGGACCACAG GGGAAGATTG GAGATCGAGG TCCAGACGGT 
TTGCCCGGCC CACTTGGTCC TGAGGGTTTT CCAGGGGATA TTGGACCACC AGGACAGAAT 
GGTGTTGAGG GCCCAAAGGG GAATGCAGGA GTGAGAGGCA TTCCAGGCCC TGGAGGACTC 
CCTGGACTCG AGGGAGATCA GGGACCAGTG GGACCAGCAG GGGCTCCAGG ACTAGAGGGC 
AGACCTGGGA GGAAAGGTAT CTCTGGCAAT CCAGGGGAGG AAGGGATGAA GGGAGAGCCT 
GGAATGACTG GAAATCCTGG GATGCTTGGT GAGAGGGGTC CTGTTGGTTT CGTCGGGCCT 
TTTGGTGAGA TGGGACTTGC TGGAGAAAAG GGAGATCGTG GTGAGACGGG TCAGCCTGGG 
CCACCTGGAG AAAAGGGAGC CATGGGTCAC CCAGGGGCTC CAGGAGAGAG AGGCCTGTCT 
GGACCAGCAG GTGCTCCAGG GCCTCATGGA TCTAGGGGCC TCAGTGGCAC CAGGGGGCCA 
AAAGGCACAA GGGGTGCAAG GGGACCAGAT GGACCAGTTG GAGAGAAAGG AATGATGGGA 
ATGAAGGGTC CTGAAGGTCC ACCAGGAAAA CAAGGTTTAA GCGGACAAAT GGGTAAAATT 
GGAGAAACTG GTGAAGCAGG ACCTACCGGA TTTACTGGTG TTCAAGGACC AACTGGACCT 
CCTGGAGCAA AAGGCATCTT AGGAGAACCT GGTCCTCAAG GATCACCGGG AGTGTTGGGT 
CCATTGGGTG AGATTGGAGC AATAGGTTTA CCTGGGAAGG CTGGCGATCA GGGCTTACCG 
GGAGAACCAG GAGAGAAGGG TGCAGTCGGG TCTCCTGGGA ATATTGGAGA GCAAGGCCTG 
ATTGGACCCC GAGGTGACCC TGGAGTCGAT GGAGAGGCTG GTCCAAGTGG TCCCGATGGA 
GCCAAGGGTG AACAGGGGGA TGTTGGACTG GAGGGAGAAT CAGGCGAAAA AGGAGTCATC 
GGATTTAAAG GAACAGAGGG ACGTACTGGT GATCCTGGCT TAATAGGCGT CAAGGGCCCT 
GAAGGCAAAA TAGGGAAAAC TGGTGAGAGG GGAAAACCTG GAGAGAAGGG AAGCAAAGGT 
CATCAAGGTC AACTTGGCGA GATGGGTGCT TTGGGTGAGC AAGGTGACAC GGGTTTCATC 
GGGCCAAAAG GAAGCAGAGG AACTACTGGA TTTATGGGTG CGACGGGAAA AATGGGTCAG 
CACGGAGAGC CAGGTTTGGT GGGATATGAA GGTCATCAAG GGCCACAAGG TTCATTGGGA 
TCCCCGGGAC CAAAAGGTGA AAAGGGAGAG CAAGGCGATG ACGGGAAGGT TGAAGGTCCA 
CCTGGTCCTT CTGGTGACAT CGGACCTGTT GGCAACAGAG GAGACAGAGG AGAACCTGGT 
GACCCTGGAT ACCCAGGCCT TGAAGGTGTT CAAGGAGAAA GAGGAAAAGA GGGGGCTCCT 
GGAGTCCCTG GGAATTCAGG GCCTAGAGGT TTTCCAGGGC CCAAAGGATC TAAAGGCAAT 
AAAGGTCCAA AAGGCAAAAA CAGTCCTCGT GGTGAATCTG GAAACCGAGG GTCTCCTGGT 
CCTGTTGGTG TTCCTGGGCC CAGAGGGGTC ATCGGTCGAG AGGGTTTTGA GGGGGATCCT 
GGACCCGATG GAGCTGCTGG GAAAGATGGA GCTAAAGGAA TGCCAGTAAG ACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211607.1
CDS	419..4195
Translation	MEPDNTPSSRLRAAGVGGRAVFFCMILYCTCCLRLAQAQSADVDVLQRLGLVGKRPPQGFIPIKSGVILTTRARIDVPVSSVIPVSLGSTFSIILSVCSHRINSAFLFTIVTKRKRLHLGVQFIPGQILVYLGQNSSVNFDYNVHNGQWHNLALEIQGQKVTLYTSCGNTSIQANLDFQTEETLDPEGSFRLGKMSQNSVQFEGAICQFDIHPSAQAAHNYCKYIKKQCREADTYRPNLPPLLPLLPLDPNTSTIQTPKVVTDFNERHLSLTRDEMNINHEGQTTVPPMVTQPTLQLPLQTTAQTTASIRNRTSQISPKPTQQNRKKAKKERNKLHILKEHQISVTDSPTSQQKNQVDIPTTTTPELSSTFRVSEMTTLAAQRPLPKETSLFEAKATFFESVTPAAMDGLQTFDLEPTQYSFLELTGLKGEPGLPGPPGPPGQPGLPGKRGPRGPSGPHGKPGPPGTPGPKGKKGNPGISPGRAPSGVKGDPGLVGLPGQPGQPGRKGQKGHPGPSGHPGDQGERGPDGSPGAKGYPGRQGLPGPIGNMGPKGVRGFIGIPGIFGLPGADGERGLPGVPGKKGKMGRPGFPGDFGERGPPGPDGNPGEIGAPGPVGIPGFIGDMGPVGMVGPPGLIGPKGVPGNPGEPGLKGDKGELGLPGEPGEPGFQGDKGIQGSPGLPGVQGKPGPQGKIGDRGPDGLPGPLGPEGFPGDIGPPGQNGVEGPKGNAGVRGIPGPGGLPGLEGDQGPVGPAGAPGLEGRPGRKGISGNPGEEGMKGEPGMTGNPGMLGERGPVGFVGPFGEMGLAGEKGDRGETGQPGPPGEKGAMGHPGAPGERGLSGPAGAPGPHGSRGLSGTRGPKGTRGARGPDGPVGEKGMMGMKGPEGPPGKQGLSGQMGKIGETGEAGPTGFTGVQGPTGPPGAKGILGEPGPQGSPGVLGPLGEIGAIGLPGKAGDQGLPGEPGEKGAVGSPGNIGEQGLIGPRGDPGVDGEAGPSGPDGAKGEQGDVGLEGESGEKGVIGFKGTEGRTGDPGLIGVKGPEGKIGKTGERGKPGEKGSKGHQGQLGEMGALGEQGDTGFIGPKGSRGTTGFMGATGKMGQHGEPGLVGYEGHQGPQGSLGSPGPKGEKGEQGDDGKVEGPPGPSGDIGPVGNRGDRGEPGDPGYPGLEGVQGERGKEGAPGVPGNSGPRGFPGPKGSKGNKGPKGKNSPRGESGNRGSPGPVGVPGPRGVIGREGFEGDPGPDGAAGKDGAKGMPVRQ

Target ORF information:

RefSeq Version	XM_017356118.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XXVII, alpha 1b (col27a1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356118.2

ORF Insert Sequence:

ATGGAGCCGG ACAATACCCC GAGCTCCAGA CTCAGAGCAG CTGGGGTCGG GGGGAGAGCA 
GTGTTTTTCT GCATGATTCT TTACTGTACA TGCTGTTTGA GGCTCGCACA AGCACAATCT 
GCAGATGTGG ACGTGCTGCA AAGACTGGGA TTGGTTGGGA AAAGACCACC TCAAGGCTTC 
ATCCCTATTA AATCAGGAGT CATCCTAACC ACAAGGGCGA GAATTGACGT CCCGGTGAGC 
TCCGTCATTC CTGTGTCACT GGGCTCGACG TTCTCTATCA TCCTCAGCGT ATGTTCCCAT 
CGCATAAACA GTGCTTTCCT TTTCACCATT GTGACAAAGA GGAAAAGACT GCACCTAGGG 
GTGCAGTTCA TCCCTGGACA GATATTAGTC TACCTAGGCC AAAACAGCTC TGTAAATTTT 
GATTACAATG TTCACAATGG CCAGTGGCAC AACCTGGCTT TGGAAATCCA AGGCCAGAAG 
GTTACTTTAT ATACTTCCTG TGGGAATACA AGCATACAGG CAAATCTGGA TTTTCAAACT 
GAGGAAACGC TGGATCCAGA GGGCTCCTTT CGCTTGGGAA AAATGAGCCA GAACTCAGTG 
CAGTTCGAAG GAGCCATCTG CCAGTTTGAC ATTCATCCCT CTGCTCAGGC AGCTCACAAT 
TATTGCAAGT ACATTAAAAA ACAATGCAGG GAGGCGGATA CTTATCGGCC AAACCTTCCT 
CCTCTTCTAC CACTATTACC CCTGGACCCT AATACATCTA CCATTCAAAC CCCCAAAGTT 
GTAACAGATT TTAACGAAAG ACATCTTTCA CTAACACGAG ATGAGATGAA TATTAATCAT 
GAAGGCCAAA CCACGGTGCC ACCAATGGTA ACACAGCCCA CATTACAGCT CCCCCTGCAG 
ACCACAGCGC AAACTACTGC CTCCATTAGG AACAGGACTT CACAAATATC TCCAAAGCCA 
ACTCAACAAA ACAGGAAGAA AGCAAAAAAA GAACGTAACA AGTTACATAT TTTAAAAGAG 
CACCAGATTT CTGTCACTGA CAGTCCAACA AGTCAACAAA AAAACCAAGT AGATATTCCA 
ACCACAACAA CACCTGAACT TTCTAGTACA TTCAGGGTGT CAGAAATGAC GACATTAGCA 
GCACAGAGAC CTCTGCCAAA GGAAACCTCC TTATTTGAAG CCAAAGCCAC CTTTTTTGAG 
TCTGTAACCC CTGCAGCTAT GGATGGTTTG CAAACGTTTG ACTTGGAACC GACCCAGTAT 
TCCTTCTTGG AGTTAACCGG ACTGAAAGGA GAACCCGGAC TACCGGGACC ACCAGGACCT 
CCGGGCCAGC CAGGATTACC GGGAAAGAGA GGTCCCCGGG GACCCTCTGG TCCGCATGGA 
AAACCAGGAC CCCCAGGGAC TCCTGGACCT AAGGGAAAAA AGGGAAATCC TGGAATCTCA 
CCTGGCAGAG CACCAAGTGG AGTAAAGGGC GACCCTGGAT TAGTAGGTCT GCCTGGACAA 
CCCGGTCAAC CCGGTCGTAA AGGACAGAAA GGCCATCCTG GTCCCTCTGG CCACCCTGGA 
GACCAAGGAG AAAGAGGACC GGATGGAAGT CCAGGTGCCA AAGGTTATCC TGGCAGGCAG 
GGCTTACCTG GGCCTATAGG AAATATGGGA CCAAAAGGCG TACGGGGCTT CATTGGAATC 
CCAGGCATCT TCGGTCTCCC TGGAGCTGAC GGAGAAAGAG GTTTACCTGG TGTTCCTGGG 
AAGAAGGGCA AGATGGGTAG GCCGGGCTTT CCAGGAGATT TTGGGGAGCG AGGACCACCA 
GGACCTGATG GAAACCCAGG GGAAATCGGC GCCCCGGGGC CTGTTGGCAT CCCTGGATTT 
ATTGGTGATA TGGGCCCTGT TGGAATGGTC GGTCCTCCTG GACTAATAGG ACCAAAGGGT 
GTCCCAGGAA ACCCAGGAGA GCCCGGACTG AAAGGTGATA AGGGTGAGCT CGGATTGCCA 
GGTGAACCCG GGGAACCTGG ATTTCAAGGT GACAAGGGTA TTCAGGGCTC ACCGGGTTTA 
CCAGGAGTTC AAGGAAAGCC AGGACCACAG GGGAAGATTG GAGATCGAGG TCCAGACGGT 
TTGCCCGGCC CACTTGGTCC TGAGGGTTTT CCAGGGGATA TTGGACCACC AGGACAGAAT 
GGTGTTGAGG GCCCAAAGGG GAATGCAGGA GTGAGAGGCA TTCCAGGCCC TGGAGGACTC 
CCTGGACTCG AGGGAGATCA GGGACCAGTG GGACCAGCAG GGGCTCCAGG ACTAGAGGGC 
AGACCTGGGA GGAAAGGTAT CTCTGGCAAT CCAGGGGAGG AAGGGATGAA GGGAGAGCCT 
GGAATGACTG GAAATCCTGG GATGCTTGGT GAGAGGGGTC CTGTTGGTTT CGTCGGGCCT 
TTTGGTGAGA TGGGACTTGC TGGAGAAAAG GGAGATCGTG GTGAGACGGG TCAGCCTGGG 
CCACCTGGAG AAAAGGGAGC CATGGGTCAC CCAGGGGCTC CAGGAGAGAG AGGCCTGTCT 
GGACCAGCAG GTGCTCCAGG GCCTCATGGA TCTAGGGGCC TCAGTGGCAC CAGGGGGCCA 
AAAGGCACAA GGGGTGCAAG GGGACCAGAT GGACCAGTTG GAGAGAAAGG AATGATGGGA 
ATGAAGGGTC CTGAAGGTCC ACCAGGAAAA CAAGGTTTAA GCGGACAAAT GGGTAAAATT 
GGAGAAACTG GTGAAGCAGG ACCTACCGGA TTTACTGGTG TTCAAGGACC AACTGGACCT 
CCTGGAGCAA AAGGCATCTT AGGAGAACCT GGTCCTCAAG GATCACCGGG AGTGTTGGGT 
CCATTGGGTG AGATTGGAGC AATAGGTTTA CCTGGGAAGG CTGGCGATCA GGGCTTACCG 
GGAGAACCAG GAGAGAAGGG TGCAGTCGGG TCTCCTGGGA ATATTGGAGA GCAAGGCCTG 
ATTGGACCCC GAGGTGACCC TGGAGTCGAT GGAGAGGCTG GTCCAAGTGG TCCCGATGGA 
GCCAAGGGTG AACAGGGGGA TGTTGGACTG GAGGGAGAAT CAGGCGAAAA AGGAGTCATC 
GGATTTAAAG GAACAGAGGG ACGTACTGGT GATCCTGGCT TAATAGGCGT CAAGGGCCCT 
GAAGGCAAAA TAGGGAAAAC TGGTGAGAGG GGAAAACCTG GAGAGAAGGG AAGCAAAGGT 
CATCAAGGTC AACTTGGCGA GATGGGTGCT TTGGGTGAGC AAGGTGACAC GGGTTTCATC 
GGGCCAAAAG GAAGCAGAGG AACTACTGGA TTTATGGGTG CGACGGGAAA AATGGGTCAG 
CACGGAGAGC CAGGTTTGGT GGGATATGAA GGTCATCAAG GGCCACAAGG TTCATTGGGA 
TCCCCGGGAC CAAAAGGTGA AAAGGGAGAG CAAGGCGATG ACGGGAAGGT TGAAGGTCCA 
CCTGGTCCTT CTGGTGACAT CGGACCTGTT GGCAACAGAG GAGACAGAGG AGAACCTGGT 
GACCCTGGAT ACCCAGGCCT TGAAGGTGTT CAAGGAGAAA GAGGAAAAGA GGGGGCTCCT 
GGAGTCCCTG GGAATTCAGG GCCTAGAGGT TTTCCAGGGC CCAAAGGATC TAAAGGCAAT 
AAAGGTCCAA AAGGCAAAAA CAGTCCTCGT GGTGAATCTG GAAACCGAGG GTCTCCTGGT 
CCTGTTGGTG TTCCTGGGCC CAGAGGGGTC ATCGGTCGAG AGGGTTTTGA GGGGGATCCT 
GGACCCGATG GAGCTGCTGG GAAAGATGGA GCTAAAGGAA TGCCAGTAAG ACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1B ( XM_017356119.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017211608.1 Danio rerio collagen, type XXVII, alpha 1b (col27a1b), transcript variant X3, mRNA.

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CloneID	ODa59100
Clone ID Related Accession (Same CDS sequence)	XM_017356119.1
Accession Version	XM_017356119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-1(XXVII) chain B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG ACAATACCCC GAGCTCCAGA CTCAGAGCAG CTGGGGTCGG GGGGAGAGCA 
GTGTTTTTCT GCATGATTCT TTACTGTACA TGCTGTTTGA GGCTCGCACA AGCACAATCT 
GCAGATGTGG ACGTGCTGCA AAGACTGGGA TTGGTTGGGA AAAGACCACC TCAAGGCTTC 
ATCCCTATTA AATCAGGAGT CATCCTAACC ACAAGGGCGA GAATTGACGT CCCGGTGAGC 
TCCGTCATTC CTGTGTCACT GGGCTCGACG TTCTCTATCA TCCTCAGCGT ATGTTCCCAT 
CGCATAAACA GTGCTTTCCT TTTCACCATT GTGACAAAGA GGAAAAGACT GCACCTAGGG 
GTGCAGTTCA TCCCTGGACA GATATTAGTC TACCTAGGCC AAAACAGCTC TGTAAATTTT 
GATTACAATG TTCACAATGG CCAGTGGCAC AACCTGGCTT TGGAAATCCA AGGCCAGAAG 
GTTACTTTAT ATACTTCCTG TGGGAATACA AGCATACAGG CAAATCTGGA TTTTCAAACT 
GAGGAAACGC TGGATCCAGA GGGCTCCTTT CGCTTGGGAA AAATGAGCCA GAACTCAGTG 
CAGTTCGAAG GAGCCATCTG CCAGTTTGAC ATTCATCCCT CTGCTCAGGC AGCTCACAAT 
TATTGCAAGT ACATTAAAAA ACAATGCAGG GAGGCGGATA CTTATCGGCC AAACCTTCCT 
CCTCTTCTAC CACTATTACC CCTGGACCCT AATACATCTA CCATTCAAAC CCCCAAAGTT 
GTAACAGATT TTAACGAAAG ACATCTTTCA CTAACACGAG ATGAGATGAA TATTAATCAT 
GAAGGCCAAA CCACGGTGCC ACCAATGGTA ACACAGCCCA CATTACAGCT CCCCCTGCAG 
ACCACAGCGC AAACTACTGC CTCCATTAGG AACAGGACTT CACAAATATC TCCAAAGCCA 
ACTCAACAAA ACAGGAAGAA AGCAAAAAAA GAACGTAACA AGTTACATAT TTTAAAAGAG 
CACCAGATTT CTGTCACTGA CAGTCCAACA AGTCAACAAA AAAACCAAGT AGATATTCCA 
ACCACAACAA CACCTGAACT TTCTAGTACA TTCAGGGTGT CAGAAATGAC GACATTAGCA 
GCACAGAGAC CTCTGCCAAA GGAAACCTCC TTATTTGAAG CCAAAGCCAC CTTTTTTGAG 
TCTGTAACCC CTGCAGCTAT GGATGGTTTG CAAACGTTTG ACTTGGAACC GACCCAGTAT 
TCCTTCTTGG AGTTAACCGG ACTGAAAGGA GAACCCGGAC TACCGGGACC ACCAGGACCT 
CCGGGCCAGC CAGGATTACC GGGAAAGAGA GGTCCCCGGG GACCCTCTGG TCCGCATGGA 
AAACCAGGAC CCCCAGGGAC TCCTGGACCT AAGGGAAAAA AGGGAAATCC TGGAATCTCA 
CCTGGCAGAG CACCAAGTGG AGTAAAGGGC GACCCTGGAT TAGTAGGTCT GCCTGGACAA 
CCCGGTCAAC CCGGTCGTAA AGGACAGAAA GGCCATCCTG GTCCCTCTGG CCACCCTGGA 
GACCAAGGAG AAAGAGGACC GGATGGAAGT CCAGGTGCCA AAGGTTATCC TGGCAGGCAG 
GGCTTACCTG GGCCTATAGG AAATATGGGA CCAAAAGGCG TACGGGGCTT CATTGGAATC 
CCAGGCATCT TCGGTCTCCC TGGAGCTGAC GGAGAAAGAG GTTTACCTGG TGTTCCTGGG 
AAGAAGGGCA AGATGGGTAG GCCGGGCTTT CCAGGAGATT TTGGGGAGCG AGGACCACCA 
GGACCTGATG GAAACCCAGG GGAAATCGGC GCCCCGGGGC CTGTTGGCAT CCCTGGATTT 
ATTGGTGATA TGGGCCCTGT TGGAATGGTC GGTCCTCCTG GACTAATAGG ACCAAAGGGT 
GTCCCAGGAA ACCCAGGAGA GCCCGGACTG AAAGGTGATA AGGGTGAGCT CGGATTGCCA 
GGTGAACCCG GGGAACCTGG ATTTCAAGGT GACAAGGGTA TTCAGGGCTC ACCGGGTTTA 
CCAGGAGTTC AAGGAAAGCC AGGACCACAG GGGAAGATTG GAGATCGAGG TCCAGACGGT 
TTGCCCGGCC CACTTGGTCC TGAGGGTTTT CCAGGGGATA TTGGACCACC AGGACAGAAT 
GGTGTTGAGG GCCCAAAGGG GAATGCAGGA GTGAGAGGCA TTCCAGGCCC TGGAGGACTC 
CCTGGACTCG AGGGAGATCA GGGACCAGTG GGACCAGCAG GGGCTCCAGG ACTAGAGGGC 
AGACCTGGGA GGAAAGGTAT CTCTGGCAAT CCAGGGGAGG AAGGGATGAA GGGAGAGCCT 
GGAATGACTG GAAATCCTGG GATGCTTGGT GAGAGGGGTC CTGTTGGTTT CGTCGGGCCT 
TTTGGTGAGA TGGGACTTGC TGGAGAAAAG GGAGATCGTG GTGAGACGGG TCAGCCTGGG 
CCACCTGGAG AAAAGGGAGC CATGGGTCAC CCAGGGGCTC CAGGAGAGAG AGGCCTGTCT 
GGACCAGCAG GTGCTCCAGG GCCTCATGGA TCTAGGGGCC TCAGTGGCAC CAGGGGGCCA 
AAAGGCACAA GGGGTGCAAG GGGACCAGAT GGACCAGTTG GAGAGAAAGG AATGATGGGA 
ATGAAGGGTC CTGAAGGTCC ACCAGGAAAA CAAGGTTTAA GCGGACAAAT GGGTAAAATT 
GGAGAAACTG GTGAAGCAGG ACCTACCGGA TTTACTGGTG TTCAAGGACC AACTGGACCT 
CCTGGAGCAA AAGGCATCTT AGGAGAACCT GGTCCTCAAG GATCACCGGG AGTGTTGGGT 
CCATTGGGTG AGATTGGAGC AATAGGTTTA CCTGGGAAGG CTGGCGATCA GGGCTTACCG 
GGAGAACCAG GAGAGAAGGG TGCAGTCGGG TCTCCTGGGA ATATTGGAGA GCAAGGCCTG 
ATTGGACCCC GAGGTGACCC TGGAGTCGAT GGAGAGGCTG GTCCAAGTGG TCCCGATGGA 
GCCAAGGGTG AACAGGGGGA TGTTGGACTG GAGGGAGAAT CAGGCGAAAA AGGAGTCATC 
GGATTTAAAG GAACAGAGGG ACGTACTGGT GATCCTGGCT TAATAGGCGT CAAGGGCCCT 
GAAGGCAAAA TAGGGAAAAC TGGTGAGAGG GGAAAACCTG GAGAGAAGGG AAGCAAAGGT 
CATCAAGGTC AACTTGGCGA GATGGGTGCT TTGGGTGAGC AAGGTGACAC GGGTTTCATC 
GGGCCAAAAG GAAGCAGAGG AACTACTGGA TTTATGGGTG CGACGGGAAA AATGGGTCAG 
CACGGAGAGC CAGGTTTGGT GGGATATGAA GGTCATCAAG GGCCACAAGG TTCATTGGGA 
TCCCCGGGAC CAAAAGGTGA AAAGGGAGAG CAAGGCGATG ACGGGAAGGT TGAAGGTCCA 
CCTGGTCCTT CTGGTGACAT CGTGAGGACC TGTTGGCAAC AGAGGAGACA GAGGAGAACC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211608.1
CDS	375..3860
Translation	MEPDNTPSSRLRAAGVGGRAVFFCMILYCTCCLRLAQAQSADVDVLQRLGLVGKRPPQGFIPIKSGVILTTRARIDVPVSSVIPVSLGSTFSIILSVCSHRINSAFLFTIVTKRKRLHLGVQFIPGQILVYLGQNSSVNFDYNVHNGQWHNLALEIQGQKVTLYTSCGNTSIQANLDFQTEETLDPEGSFRLGKMSQNSVQFEGAICQFDIHPSAQAAHNYCKYIKKQCREADTYRPNLPPLLPLLPLDPNTSTIQTPKVVTDFNERHLSLTRDEMNINHEGQTTVPPMVTQPTLQLPLQTTAQTTASIRNRTSQISPKPTQQNRKKAKKERNKLHILKEHQISVTDSPTSQQKNQVDIPTTTTPELSSTFRVSEMTTLAAQRPLPKETSLFEAKATFFESVTPAAMDGLQTFDLEPTQYSFLELTGLKGEPGLPGPPGPPGQPGLPGKRGPRGPSGPHGKPGPPGTPGPKGKKGNPGISPGRAPSGVKGDPGLVGLPGQPGQPGRKGQKGHPGPSGHPGDQGERGPDGSPGAKGYPGRQGLPGPIGNMGPKGVRGFIGIPGIFGLPGADGERGLPGVPGKKGKMGRPGFPGDFGERGPPGPDGNPGEIGAPGPVGIPGFIGDMGPVGMVGPPGLIGPKGVPGNPGEPGLKGDKGELGLPGEPGEPGFQGDKGIQGSPGLPGVQGKPGPQGKIGDRGPDGLPGPLGPEGFPGDIGPPGQNGVEGPKGNAGVRGIPGPGGLPGLEGDQGPVGPAGAPGLEGRPGRKGISGNPGEEGMKGEPGMTGNPGMLGERGPVGFVGPFGEMGLAGEKGDRGETGQPGPPGEKGAMGHPGAPGERGLSGPAGAPGPHGSRGLSGTRGPKGTRGARGPDGPVGEKGMMGMKGPEGPPGKQGLSGQMGKIGETGEAGPTGFTGVQGPTGPPGAKGILGEPGPQGSPGVLGPLGEIGAIGLPGKAGDQGLPGEPGEKGAVGSPGNIGEQGLIGPRGDPGVDGEAGPSGPDGAKGEQGDVGLEGESGEKGVIGFKGTEGRTGDPGLIGVKGPEGKIGKTGERGKPGEKGSKGHQGQLGEMGALGEQGDTGFIGPKGSRGTTGFMGATGKMGQHGEPGLVGYEGHQGPQGSLGSPGPKGEKGEQGDDGKVEGPPGPSGDIVRTCWQQRRQRRTW

Target ORF information:

RefSeq Version	XM_017356119.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XXVII, alpha 1b (col27a1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356119.1

ORF Insert Sequence:

ATGGAGCCGG ACAATACCCC GAGCTCCAGA CTCAGAGCAG CTGGGGTCGG GGGGAGAGCA 
GTGTTTTTCT GCATGATTCT TTACTGTACA TGCTGTTTGA GGCTCGCACA AGCACAATCT 
GCAGATGTGG ACGTGCTGCA AAGACTGGGA TTGGTTGGGA AAAGACCACC TCAAGGCTTC 
ATCCCTATTA AATCAGGAGT CATCCTAACC ACAAGGGCGA GAATTGACGT CCCGGTGAGC 
TCCGTCATTC CTGTGTCACT GGGCTCGACG TTCTCTATCA TCCTCAGCGT ATGTTCCCAT 
CGCATAAACA GTGCTTTCCT TTTCACCATT GTGACAAAGA GGAAAAGACT GCACCTAGGG 
GTGCAGTTCA TCCCTGGACA GATATTAGTC TACCTAGGCC AAAACAGCTC TGTAAATTTT 
GATTACAATG TTCACAATGG CCAGTGGCAC AACCTGGCTT TGGAAATCCA AGGCCAGAAG 
GTTACTTTAT ATACTTCCTG TGGGAATACA AGCATACAGG CAAATCTGGA TTTTCAAACT 
GAGGAAACGC TGGATCCAGA GGGCTCCTTT CGCTTGGGAA AAATGAGCCA GAACTCAGTG 
CAGTTCGAAG GAGCCATCTG CCAGTTTGAC ATTCATCCCT CTGCTCAGGC AGCTCACAAT 
TATTGCAAGT ACATTAAAAA ACAATGCAGG GAGGCGGATA CTTATCGGCC AAACCTTCCT 
CCTCTTCTAC CACTATTACC CCTGGACCCT AATACATCTA CCATTCAAAC CCCCAAAGTT 
GTAACAGATT TTAACGAAAG ACATCTTTCA CTAACACGAG ATGAGATGAA TATTAATCAT 
GAAGGCCAAA CCACGGTGCC ACCAATGGTA ACACAGCCCA CATTACAGCT CCCCCTGCAG 
ACCACAGCGC AAACTACTGC CTCCATTAGG AACAGGACTT CACAAATATC TCCAAAGCCA 
ACTCAACAAA ACAGGAAGAA AGCAAAAAAA GAACGTAACA AGTTACATAT TTTAAAAGAG 
CACCAGATTT CTGTCACTGA CAGTCCAACA AGTCAACAAA AAAACCAAGT AGATATTCCA 
ACCACAACAA CACCTGAACT TTCTAGTACA TTCAGGGTGT CAGAAATGAC GACATTAGCA 
GCACAGAGAC CTCTGCCAAA GGAAACCTCC TTATTTGAAG CCAAAGCCAC CTTTTTTGAG 
TCTGTAACCC CTGCAGCTAT GGATGGTTTG CAAACGTTTG ACTTGGAACC GACCCAGTAT 
TCCTTCTTGG AGTTAACCGG ACTGAAAGGA GAACCCGGAC TACCGGGACC ACCAGGACCT 
CCGGGCCAGC CAGGATTACC GGGAAAGAGA GGTCCCCGGG GACCCTCTGG TCCGCATGGA 
AAACCAGGAC CCCCAGGGAC TCCTGGACCT AAGGGAAAAA AGGGAAATCC TGGAATCTCA 
CCTGGCAGAG CACCAAGTGG AGTAAAGGGC GACCCTGGAT TAGTAGGTCT GCCTGGACAA 
CCCGGTCAAC CCGGTCGTAA AGGACAGAAA GGCCATCCTG GTCCCTCTGG CCACCCTGGA 
GACCAAGGAG AAAGAGGACC GGATGGAAGT CCAGGTGCCA AAGGTTATCC TGGCAGGCAG 
GGCTTACCTG GGCCTATAGG AAATATGGGA CCAAAAGGCG TACGGGGCTT CATTGGAATC 
CCAGGCATCT TCGGTCTCCC TGGAGCTGAC GGAGAAAGAG GTTTACCTGG TGTTCCTGGG 
AAGAAGGGCA AGATGGGTAG GCCGGGCTTT CCAGGAGATT TTGGGGAGCG AGGACCACCA 
GGACCTGATG GAAACCCAGG GGAAATCGGC GCCCCGGGGC CTGTTGGCAT CCCTGGATTT 
ATTGGTGATA TGGGCCCTGT TGGAATGGTC GGTCCTCCTG GACTAATAGG ACCAAAGGGT 
GTCCCAGGAA ACCCAGGAGA GCCCGGACTG AAAGGTGATA AGGGTGAGCT CGGATTGCCA 
GGTGAACCCG GGGAACCTGG ATTTCAAGGT GACAAGGGTA TTCAGGGCTC ACCGGGTTTA 
CCAGGAGTTC AAGGAAAGCC AGGACCACAG GGGAAGATTG GAGATCGAGG TCCAGACGGT 
TTGCCCGGCC CACTTGGTCC TGAGGGTTTT CCAGGGGATA TTGGACCACC AGGACAGAAT 
GGTGTTGAGG GCCCAAAGGG GAATGCAGGA GTGAGAGGCA TTCCAGGCCC TGGAGGACTC 
CCTGGACTCG AGGGAGATCA GGGACCAGTG GGACCAGCAG GGGCTCCAGG ACTAGAGGGC 
AGACCTGGGA GGAAAGGTAT CTCTGGCAAT CCAGGGGAGG AAGGGATGAA GGGAGAGCCT 
GGAATGACTG GAAATCCTGG GATGCTTGGT GAGAGGGGTC CTGTTGGTTT CGTCGGGCCT 
TTTGGTGAGA TGGGACTTGC TGGAGAAAAG GGAGATCGTG GTGAGACGGG TCAGCCTGGG 
CCACCTGGAG AAAAGGGAGC CATGGGTCAC CCAGGGGCTC CAGGAGAGAG AGGCCTGTCT 
GGACCAGCAG GTGCTCCAGG GCCTCATGGA TCTAGGGGCC TCAGTGGCAC CAGGGGGCCA 
AAAGGCACAA GGGGTGCAAG GGGACCAGAT GGACCAGTTG GAGAGAAAGG AATGATGGGA 
ATGAAGGGTC CTGAAGGTCC ACCAGGAAAA CAAGGTTTAA GCGGACAAAT GGGTAAAATT 
GGAGAAACTG GTGAAGCAGG ACCTACCGGA TTTACTGGTG TTCAAGGACC AACTGGACCT 
CCTGGAGCAA AAGGCATCTT AGGAGAACCT GGTCCTCAAG GATCACCGGG AGTGTTGGGT 
CCATTGGGTG AGATTGGAGC AATAGGTTTA CCTGGGAAGG CTGGCGATCA GGGCTTACCG 
GGAGAACCAG GAGAGAAGGG TGCAGTCGGG TCTCCTGGGA ATATTGGAGA GCAAGGCCTG 
ATTGGACCCC GAGGTGACCC TGGAGTCGAT GGAGAGGCTG GTCCAAGTGG TCCCGATGGA 
GCCAAGGGTG AACAGGGGGA TGTTGGACTG GAGGGAGAAT CAGGCGAAAA AGGAGTCATC 
GGATTTAAAG GAACAGAGGG ACGTACTGGT GATCCTGGCT TAATAGGCGT CAAGGGCCCT 
GAAGGCAAAA TAGGGAAAAC TGGTGAGAGG GGAAAACCTG GAGAGAAGGG AAGCAAAGGT 
CATCAAGGTC AACTTGGCGA GATGGGTGCT TTGGGTGAGC AAGGTGACAC GGGTTTCATC 
GGGCCAAAAG GAAGCAGAGG AACTACTGGA TTTATGGGTG CGACGGGAAA AATGGGTCAG 
CACGGAGAGC CAGGTTTGGT GGGATATGAA GGTCATCAAG GGCCACAAGG TTCATTGGGA 
TCCCCGGGAC CAAAAGGTGA AAAGGGAGAG CAAGGCGATG ACGGGAAGGT TGAAGGTCCA 
CCTGGTCCTT CTGGTGACAT CGTGAGGACC TGTTGGCAAC AGAGGAGACA GAGGAGAACC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1B ( NM_001080575.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001074044.1 Danio rerio collagen, type XXVII, alpha 1b (col27a1b), mRNA.

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CloneID	ODa38392
Clone ID Related Accession (Same CDS sequence)	NM_001080575.1
Accession Version	NM_001080575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574438400000
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(XXVII) chain B precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF032484.1. On Mar 11, 2007 this sequence version replaced XM_683038.2. ##Evidence-Data-START## Transcript exon combination :: EF032484.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCCGG ACAATACCCC GAGCTCCAGA CTCAGAGCAG CTGGGGTCGG GGGGAGAGCA 
GTGTTTTTCT GCATGGTTCT TTACTGTACA TGCTGTTTGA GGCTCGCACA AGCACAATCT 
GCAGATGTGG ACGTGCTGCA AAGACTGGGA TTGGTTGGGA AAAGACCACC TCAAGGCTTC 
ATCCCTATTA AATCAGGAGT CATCCTAACC ACAAGGGCAA GAATTGACGT CCCGGTGAGC 
TCCGTCATTC CTGTGTCACT GGGCTCGACG TTCTCTATCA TCCTCAGCGT ATGTTCCCAT 
CGCATAAACA ATGCTTTCCT TTTCACCATT GTGACAAAGA GGAAAAGACT GCACCTAGGG 
GTGCAGTTCA TCCCTGGACA GATATTAGTC TACCTAGGCC AAAACAGCTC TGTAAATTTT 
GATTACAATG TTCACAATGG CCAGTGGCAC AACCTGGCTT TGGAAATCCA AGGCCAAAAG 
GTTACTTTAT ATACTTCCTG TGGGAATACA AGCATACAGG CAAATCTGGA TTTTCAAAAT 
GAGGAAACGC TGGATTCAGA GGGCTCCTTT CGCTTGGGAA AAATGAGCCA GAACTCAGTG 
CAGTTCGAAG GAGCCATCTG CCAGTTTGAC ATTCATCCCT CTGCTCAGGC AGCTCACAAT 
TATTGCAAGT ACATTAAAAA ACAATGCAGG GAGGCGGATA CTTATCGGCC AAACCTTCCT 
CCTCTTCTAC CACTATTACC CCTGGACCCT AATAGATCTA CCATTCAAAC TCCCAAAGTT 
GTAACAGATA TCAACGAAAG ACATCTTTCA CTAACACGAG ATAAGATGAA TATTAATCAT 
GAAGGCCAAA CCACGGTGCC ACCAATGATA ACCCAGCCCA CACTACAGCT CCCCCTGCAG 
ACCACAGCGC AAACTACTGC CTCCATTAGG AACAGGACTT CACAAATATC TCCGAAGCCA 
ACTCAACAAA ACAGGAAGAA AGCAAAAAAA GAACGTAACA AGTTACATAT TTTAAAAGAG 
CACCAGATTT CTGTCACTGA CAGTCCAACA AGTCAACAAC AAAACCAAGT AGATATTCCA 
ACCACAACAA CACCTGAACT TTCTAGTACA TTCAGGGTGT CAGAAATGAC GACATTAGCA 
GCACAGAGAC CTCTGCCAAA GGAAACCTCC TTTTTTGAAG CCAAAGCCAC CTTTTTTGAG 
TCTGTAACCC CTGCAGCTAT GGATGGTTTG CAAACTTTTG ACTTGGAACC GACCCAGTAT 
TCCCTCTTGG AGTTAACTGG ACTGAAAGGA GAACCCGGAC TACCGGGACC ACCAGGACCT 
CCGGGCCAGC CAGGATTACC GGGAAAGAGA GGTCCCCGGG GTCCCTCTGG TCCGCATGGA 
AAACCAGGAC CCCCAGGGAC TCCTGGACCT AAGGGAAAAA AGGGAAATCC TGGAATCTCA 
CCTGGCAGAG CACCAAGTGG AATAAAGGGC GACCCTGGAT TAGTAGGTCT GCCTGGACAA 
CCCGGTCAAC CCGGTCGTAA AGGACAGAAA GGCCATCCTG GTCCCTCTGG CCACCCTGGA 
GACCAAGGAA TAAGAGGACC GGATGGAAGT CCAGGTGCCA AAGGTTATCC TGGCAGGCAG 
GGCTTACCTG GGCCTATAGG AAATATGGGA CCAAAAGGCG TACGGGGCTT CATTGGAATC 
CCAGGCATCT TCGGTCTCCC TGGAGCTGAC GGAGAAAGAG GTTTACCCGG TGTTCCTGGG 
AAGAAGGGCA AGATGGGTAG GCCGGGCTTT CCAGGAGATT TTGGGGAGCG AGGACCACCA 
GGACCTGATG GAAACCCAGG GGAAATCGGC GCCCCGGGGC CTGTTGGCAT CCCTGGATTT 
ATTGGTGATA TGGGCCCTGT TGGAATGGTC GGTCCTCCTG GACTAATAGG ACCAAAGGGT 
GTCCCAGGAA ACCCAGGAGA GCCCGGACTG AAAGGTGATA AGGGTGAGCT CGGATTGCCA 
GGTGAACCCG GGGAACCTGG ATTTCAAGGT GACAAGGGTA TTCAGGGCTC ACCGGGTTTA 
CCAGGAGTTC AAGGAAAGCC AGGACCACAG GGGAAGATTG GAGATCGAGG TCCAGACGGT 
TTGCCCGGCC CACTTGGTCC TGAGGGTTTT CCAGGGGATA TTGGACCACC AGGACAGAAT 
GGTGTTGAGG GCCCAAAGGG GAATGCAGGA GTGAGAGGCA TTCCAGGCCC TGGAGGACTC 
CCTGGACTCG AGGGAGATCA GGGACCAGTG GGACCAGCAG GGGCTCCAGG ACTAGAGGGC 
AGACCTGGGA GGAAAGGTAT CTCTGGCAAT CCAGGGGAGG AAGGGATGAA GGGAGAGCCT 
GGAATGACTG GAAATCCTGG GATGCTTGGT GAGAGGGGTC CTGTTGGTTT CGTCGGGCCT 
TTTGGTGAGA TGGGACTTGC TGGAGAAAAG GGAGATCGTG GTGAGACGGG TCAGCCTGGG 
CCACCTGGAG AAAAGGGAGC CATGGGTCAC CCAGGGGCTC CAGGAGAGAG AGGCCTGTCT 
GGACCAGCAG GTGCTCCAGG GCCTCATGGA TCTAGGGGCC TCAGTGGCAC CAGGGGGCCA 
AAAGGCACAA GGGGTGCAAG GGGACCAGAT GGACCAGTTG GAGAGAAAGG AATGATGGGA 
ATGAAGGGTC CTGAAGGTCC ACCAGGAAAA CAAGGTTTAA GCGGACAAAT GGGTAAAATT 
GGAGAAACTG GTGAAGCAGG ACCTACCGGA TTTACTGGTG TTCAAGGACC AACTGGACCT 
CCTGGAGCAA AAGGCATCTT AGGAGAACCT GGTCCTCAAG GATCACCGGG AGTGTTGGGT 
CCATTGGGTG AGATTGGAGC AATAGGTTTA CCTGGGAAGG CTGGCGATCA GGGCTTACCG 
GGAGAACCAG GAGAGAAGGG TGCAGTCGGG TCTCCTGGGA ATATTGGAGA GCAAGGCCTG 
ATTGGACCTC GAGGTGACCC TGGAGTCGAT GGAGAGGCTG GTCCAAGTGG TCCCGATGGA 
GCCAAGGGTG AACAGGGGGA TGTTGGACTG GAGGGAGAAT CAGGCGAAAA AGGAGTCATC 
GGATTTAAAG GAACAGAGGG ACGTACTGGT GATCCTGGCT TAATAGGCGT CAAGGGCCCT 
GAAGGCAAAC CAGGGAAAAT TGGTGAGAGG GGAAAACCTG GAGAGAAGGG CAGCAAAGGT 
CATCAAGGTC AACTTGGCGA GATGGGTGCT TTGGGTGAGC AAGGTGACAC GGGTTTCATC 
GGGCCAAAAG GAAGCAGAGG AACTACTGGA TTTATGGGTG CTCCGGGAAA AATGGGTCAG 
CAGGGAGAGC CAGGTTTGGT GGGATATGAA GGTCATCAAG GGCCACAAGG TTCATTGGGA 
TCCCCGGGAC CAAAAGGTGA AAAGGGAGAG CAAGGCGATG ACGGGAAGGT TGAAGGTCCA 
CCTGGTCCTT CTGGTGACAT CGGACCTGTT GGCAACAGAG GAGACAGAGG AGAACCTGGT 
GACCCTGGAT ACCCAGGCCT TGAAGGTGTT CAAGGAGAAA GAGGAAAAGA GGGGGCTCCT 
GGAGTCCCTG GGAATTCAGG GCCTAGAGGT TTTCCAGGGC CCAAAGGATC TAAAGGCAAT 
AAAGGTCCAA AAGGCAAAAA CAGTCCTCGT GGTGAATCTG GAAACCGAGG GTCTCCTGGT 
CCTGTTGGTG TTCCTGGGCC CAGAGGGGTC ATTGGTAGAG AGGGTTTTGA GGGGGAACCT 
GGACTGGATG GAGCTGCTGG GAAAGATGGA GCTAAAGGAA TGCCAGGTGA TCTTGGACGT 
GATGGGGATG TTGGGCTGCC TGGAAAACCG GGTCCTCAAG GAAATGCAGG AGCACCAGGT 
CTACCAGGAG TTCAAGGCTC TTTTGGGCCA AAGGGGGAAA GGGGCATCAC TGGTCATTCT 
GGACCTCCAG GCAAAAGAGG ACTGAACGGA GGAATGGGAT TTCCTGGAAA ACAAGGCGAC 
CAAGGCTTCA AAGGACAACC TGGTGATGCT GGTGAACAGG GATTTCCTGG AGTTCTTGGA 
ATATTTGGAC CACAGGGGCC TCCTGGAGAC TTCGGTCCAG TGGGTATACA GGGTCCAAAG 
GGTCCTCAGG GTTTAATGGG GATGCAAGGA GCCATCGGAC CAGTTGGGAT CATTGGGCCG 
AGTGGAAACC CAGGACCACA AGGAGACAAA GGAAACAAAG GGGAAATGGG TGTTCAGGGA 
CCCAGGGGGC CTCCAGGTCC TCGTGGACCA CCGGGACCTC CAGGGCTTCC TGCTGTAGCC 
TTCTCTCATG AAAATGAGGC CCTTGGTGCT GCTTTACATG TTTTTGATTC CCGCTCCGCA 
TTAAGATCTG AAATGTATCA GGATACTGAC CTTCCACTGC TGGATCAAGG AAGCGAGATT 
TTTAAGACTC TTCACTACCT CAGCATCCTG ATCCACAGTA TCAAAAACCC GCTGGGAACG 
CAGGAGAATC CGGCCAGAAT GTGCAGGGAT CTGCTTGAAT GCGAGCACCG GTTGAATGAT 
GGCACTTATT GGATAGATCC TAACCTGGGC TGCTCCTCTG ACAACATTGA AGTGACCTGT 
AGCTTCACCA GTGGAGGTCA AACCTGCCTT AAGCCAGTCA CTGCTTCCAA GCTGGAGATC 
GGAGTCAGTC TGATCCAGAT GAACTTCATC CACCTCCTGA GCTCTGAGGC TGTGCAGATC 
ATCACCGTTC ACTGTCTGAA CGTCTCCGTT TGGGCATCGG AAGACTCCAA AACACCTTCC 
TCCAGCATGG TATACTTTAA AGCCTGGGAT GGACAGATAA TCGAGGCTGG AGGATTCATC 
GAGCCTGATT TGCTGAAGGA TGAGTGCTGG ATCACAGATG GTAGATGGCA TCAAACACAG 
TTCATTTTCC GGACGCAGGA CCCAAACTTA CTACCCATTG TGGAGATCTA CAACTTGCCC 
AGCACTAAAC CTGGCTCACA TTACCACCTG GAGGTGGGCC CTGTGTGCTT TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074044.1
CDS	1..4977
Translation	MEPDNTPSSRLRAAGVGGRAVFFCMVLYCTCCLRLAQAQSADVDVLQRLGLVGKRPPQGFIPIKSGVILTTRARIDVPVSSVIPVSLGSTFSIILSVCSHRINNAFLFTIVTKRKRLHLGVQFIPGQILVYLGQNSSVNFDYNVHNGQWHNLALEIQGQKVTLYTSCGNTSIQANLDFQNEETLDSEGSFRLGKMSQNSVQFEGAICQFDIHPSAQAAHNYCKYIKKQCREADTYRPNLPPLLPLLPLDPNRSTIQTPKVVTDINERHLSLTRDKMNINHEGQTTVPPMITQPTLQLPLQTTAQTTASIRNRTSQISPKPTQQNRKKAKKERNKLHILKEHQISVTDSPTSQQQNQVDIPTTTTPELSSTFRVSEMTTLAAQRPLPKETSFFEAKATFFESVTPAAMDGLQTFDLEPTQYSLLELTGLKGEPGLPGPPGPPGQPGLPGKRGPRGPSGPHGKPGPPGTPGPKGKKGNPGISPGRAPSGIKGDPGLVGLPGQPGQPGRKGQKGHPGPSGHPGDQGIRGPDGSPGAKGYPGRQGLPGPIGNMGPKGVRGFIGIPGIFGLPGADGERGLPGVPGKKGKMGRPGFPGDFGERGPPGPDGNPGEIGAPGPVGIPGFIGDMGPVGMVGPPGLIGPKGVPGNPGEPGLKGDKGELGLPGEPGEPGFQGDKGIQGSPGLPGVQGKPGPQGKIGDRGPDGLPGPLGPEGFPGDIGPPGQNGVEGPKGNAGVRGIPGPGGLPGLEGDQGPVGPAGAPGLEGRPGRKGISGNPGEEGMKGEPGMTGNPGMLGERGPVGFVGPFGEMGLAGEKGDRGETGQPGPPGEKGAMGHPGAPGERGLSGPAGAPGPHGSRGLSGTRGPKGTRGARGPDGPVGEKGMMGMKGPEGPPGKQGLSGQMGKIGETGEAGPTGFTGVQGPTGPPGAKGILGEPGPQGSPGVLGPLGEIGAIGLPGKAGDQGLPGEPGEKGAVGSPGNIGEQGLIGPRGDPGVDGEAGPSGPDGAKGEQGDVGLEGESGEKGVIGFKGTEGRTGDPGLIGVKGPEGKPGKIGERGKPGEKGSKGHQGQLGEMGALGEQGDTGFIGPKGSRGTTGFMGAPGKMGQQGEPGLVGYEGHQGPQGSLGSPGPKGEKGEQGDDGKVEGPPGPSGDIGPVGNRGDRGEPGDPGYPGLEGVQGERGKEGAPGVPGNSGPRGFPGPKGSKGNKGPKGKNSPRGESGNRGSPGPVGVPGPRGVIGREGFEGEPGLDGAAGKDGAKGMPGDLGRDGDVGLPGKPGPQGNAGAPGLPGVQGSFGPKGERGITGHSGPPGKRGLNGGMGFPGKQGDQGFKGQPGDAGEQGFPGVLGIFGPQGPPGDFGPVGIQGPKGPQGLMGMQGAIGPVGIIGPSGNPGPQGDKGNKGEMGVQGPRGPPGPRGPPGPPGLPAVAFSHENEALGAALHVFDSRSALRSEMYQDTDLPLLDQGSEIFKTLHYLSILIHSIKNPLGTQENPARMCRDLLECEHRLNDGTYWIDPNLGCSSDNIEVTCSFTSGGQTCLKPVTASKLEIGVSLIQMNFIHLLSSEAVQIITVHCLNVSVWASEDSKTPSSSMVYFKAWDGQIIEAGGFIEPDLLKDECWITDGRWHQTQFIFRTQDPNLLPIVEIYNLPSTKPGSHYHLEVGPVCFL

Target ORF information:

RefSeq Version	NM_001080575.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XXVII, alpha 1b (col27a1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080575.1

ORF Insert Sequence:

ATGGAGCCGG ACAATACCCC GAGCTCCAGA CTCAGAGCAG CTGGGGTCGG GGGGAGAGCA 
GTGTTTTTCT GCATGGTTCT TTACTGTACA TGCTGTTTGA GGCTCGCACA AGCACAATCT 
GCAGATGTGG ACGTGCTGCA AAGACTGGGA TTGGTTGGGA AAAGACCACC TCAAGGCTTC 
ATCCCTATTA AATCAGGAGT CATCCTAACC ACAAGGGCAA GAATTGACGT CCCGGTGAGC 
TCCGTCATTC CTGTGTCACT GGGCTCGACG TTCTCTATCA TCCTCAGCGT ATGTTCCCAT 
CGCATAAACA ATGCTTTCCT TTTCACCATT GTGACAAAGA GGAAAAGACT GCACCTAGGG 
GTGCAGTTCA TCCCTGGACA GATATTAGTC TACCTAGGCC AAAACAGCTC TGTAAATTTT 
GATTACAATG TTCACAATGG CCAGTGGCAC AACCTGGCTT TGGAAATCCA AGGCCAAAAG 
GTTACTTTAT ATACTTCCTG TGGGAATACA AGCATACAGG CAAATCTGGA TTTTCAAAAT 
GAGGAAACGC TGGATTCAGA GGGCTCCTTT CGCTTGGGAA AAATGAGCCA GAACTCAGTG 
CAGTTCGAAG GAGCCATCTG CCAGTTTGAC ATTCATCCCT CTGCTCAGGC AGCTCACAAT 
TATTGCAAGT ACATTAAAAA ACAATGCAGG GAGGCGGATA CTTATCGGCC AAACCTTCCT 
CCTCTTCTAC CACTATTACC CCTGGACCCT AATAGATCTA CCATTCAAAC TCCCAAAGTT 
GTAACAGATA TCAACGAAAG ACATCTTTCA CTAACACGAG ATAAGATGAA TATTAATCAT 
GAAGGCCAAA CCACGGTGCC ACCAATGATA ACCCAGCCCA CACTACAGCT CCCCCTGCAG 
ACCACAGCGC AAACTACTGC CTCCATTAGG AACAGGACTT CACAAATATC TCCGAAGCCA 
ACTCAACAAA ACAGGAAGAA AGCAAAAAAA GAACGTAACA AGTTACATAT TTTAAAAGAG 
CACCAGATTT CTGTCACTGA CAGTCCAACA AGTCAACAAC AAAACCAAGT AGATATTCCA 
ACCACAACAA CACCTGAACT TTCTAGTACA TTCAGGGTGT CAGAAATGAC GACATTAGCA 
GCACAGAGAC CTCTGCCAAA GGAAACCTCC TTTTTTGAAG CCAAAGCCAC CTTTTTTGAG 
TCTGTAACCC CTGCAGCTAT GGATGGTTTG CAAACTTTTG ACTTGGAACC GACCCAGTAT 
TCCCTCTTGG AGTTAACTGG ACTGAAAGGA GAACCCGGAC TACCGGGACC ACCAGGACCT 
CCGGGCCAGC CAGGATTACC GGGAAAGAGA GGTCCCCGGG GTCCCTCTGG TCCGCATGGA 
AAACCAGGAC CCCCAGGGAC TCCTGGACCT AAGGGAAAAA AGGGAAATCC TGGAATCTCA 
CCTGGCAGAG CACCAAGTGG AATAAAGGGC GACCCTGGAT TAGTAGGTCT GCCTGGACAA 
CCCGGTCAAC CCGGTCGTAA AGGACAGAAA GGCCATCCTG GTCCCTCTGG CCACCCTGGA 
GACCAAGGAA TAAGAGGACC GGATGGAAGT CCAGGTGCCA AAGGTTATCC TGGCAGGCAG 
GGCTTACCTG GGCCTATAGG AAATATGGGA CCAAAAGGCG TACGGGGCTT CATTGGAATC 
CCAGGCATCT TCGGTCTCCC TGGAGCTGAC GGAGAAAGAG GTTTACCCGG TGTTCCTGGG 
AAGAAGGGCA AGATGGGTAG GCCGGGCTTT CCAGGAGATT TTGGGGAGCG AGGACCACCA 
GGACCTGATG GAAACCCAGG GGAAATCGGC GCCCCGGGGC CTGTTGGCAT CCCTGGATTT 
ATTGGTGATA TGGGCCCTGT TGGAATGGTC GGTCCTCCTG GACTAATAGG ACCAAAGGGT 
GTCCCAGGAA ACCCAGGAGA GCCCGGACTG AAAGGTGATA AGGGTGAGCT CGGATTGCCA 
GGTGAACCCG GGGAACCTGG ATTTCAAGGT GACAAGGGTA TTCAGGGCTC ACCGGGTTTA 
CCAGGAGTTC AAGGAAAGCC AGGACCACAG GGGAAGATTG GAGATCGAGG TCCAGACGGT 
TTGCCCGGCC CACTTGGTCC TGAGGGTTTT CCAGGGGATA TTGGACCACC AGGACAGAAT 
GGTGTTGAGG GCCCAAAGGG GAATGCAGGA GTGAGAGGCA TTCCAGGCCC TGGAGGACTC 
CCTGGACTCG AGGGAGATCA GGGACCAGTG GGACCAGCAG GGGCTCCAGG ACTAGAGGGC 
AGACCTGGGA GGAAAGGTAT CTCTGGCAAT CCAGGGGAGG AAGGGATGAA GGGAGAGCCT 
GGAATGACTG GAAATCCTGG GATGCTTGGT GAGAGGGGTC CTGTTGGTTT CGTCGGGCCT 
TTTGGTGAGA TGGGACTTGC TGGAGAAAAG GGAGATCGTG GTGAGACGGG TCAGCCTGGG 
CCACCTGGAG AAAAGGGAGC CATGGGTCAC CCAGGGGCTC CAGGAGAGAG AGGCCTGTCT 
GGACCAGCAG GTGCTCCAGG GCCTCATGGA TCTAGGGGCC TCAGTGGCAC CAGGGGGCCA 
AAAGGCACAA GGGGTGCAAG GGGACCAGAT GGACCAGTTG GAGAGAAAGG AATGATGGGA 
ATGAAGGGTC CTGAAGGTCC ACCAGGAAAA CAAGGTTTAA GCGGACAAAT GGGTAAAATT 
GGAGAAACTG GTGAAGCAGG ACCTACCGGA TTTACTGGTG TTCAAGGACC AACTGGACCT 
CCTGGAGCAA AAGGCATCTT AGGAGAACCT GGTCCTCAAG GATCACCGGG AGTGTTGGGT 
CCATTGGGTG AGATTGGAGC AATAGGTTTA CCTGGGAAGG CTGGCGATCA GGGCTTACCG 
GGAGAACCAG GAGAGAAGGG TGCAGTCGGG TCTCCTGGGA ATATTGGAGA GCAAGGCCTG 
ATTGGACCTC GAGGTGACCC TGGAGTCGAT GGAGAGGCTG GTCCAAGTGG TCCCGATGGA 
GCCAAGGGTG AACAGGGGGA TGTTGGACTG GAGGGAGAAT CAGGCGAAAA AGGAGTCATC 
GGATTTAAAG GAACAGAGGG ACGTACTGGT GATCCTGGCT TAATAGGCGT CAAGGGCCCT 
GAAGGCAAAC CAGGGAAAAT TGGTGAGAGG GGAAAACCTG GAGAGAAGGG CAGCAAAGGT 
CATCAAGGTC AACTTGGCGA GATGGGTGCT TTGGGTGAGC AAGGTGACAC GGGTTTCATC 
GGGCCAAAAG GAAGCAGAGG AACTACTGGA TTTATGGGTG CTCCGGGAAA AATGGGTCAG 
CAGGGAGAGC CAGGTTTGGT GGGATATGAA GGTCATCAAG GGCCACAAGG TTCATTGGGA 
TCCCCGGGAC CAAAAGGTGA AAAGGGAGAG CAAGGCGATG ACGGGAAGGT TGAAGGTCCA 
CCTGGTCCTT CTGGTGACAT CGGACCTGTT GGCAACAGAG GAGACAGAGG AGAACCTGGT 
GACCCTGGAT ACCCAGGCCT TGAAGGTGTT CAAGGAGAAA GAGGAAAAGA GGGGGCTCCT 
GGAGTCCCTG GGAATTCAGG GCCTAGAGGT TTTCCAGGGC CCAAAGGATC TAAAGGCAAT 
AAAGGTCCAA AAGGCAAAAA CAGTCCTCGT GGTGAATCTG GAAACCGAGG GTCTCCTGGT 
CCTGTTGGTG TTCCTGGGCC CAGAGGGGTC ATTGGTAGAG AGGGTTTTGA GGGGGAACCT 
GGACTGGATG GAGCTGCTGG GAAAGATGGA GCTAAAGGAA TGCCAGGTGA TCTTGGACGT 
GATGGGGATG TTGGGCTGCC TGGAAAACCG GGTCCTCAAG GAAATGCAGG AGCACCAGGT 
CTACCAGGAG TTCAAGGCTC TTTTGGGCCA AAGGGGGAAA GGGGCATCAC TGGTCATTCT 
GGACCTCCAG GCAAAAGAGG ACTGAACGGA GGAATGGGAT TTCCTGGAAA ACAAGGCGAC 
CAAGGCTTCA AAGGACAACC TGGTGATGCT GGTGAACAGG GATTTCCTGG AGTTCTTGGA 
ATATTTGGAC CACAGGGGCC TCCTGGAGAC TTCGGTCCAG TGGGTATACA GGGTCCAAAG 
GGTCCTCAGG GTTTAATGGG GATGCAAGGA GCCATCGGAC CAGTTGGGAT CATTGGGCCG 
AGTGGAAACC CAGGACCACA AGGAGACAAA GGAAACAAAG GGGAAATGGG TGTTCAGGGA 
CCCAGGGGGC CTCCAGGTCC TCGTGGACCA CCGGGACCTC CAGGGCTTCC TGCTGTAGCC 
TTCTCTCATG AAAATGAGGC CCTTGGTGCT GCTTTACATG TTTTTGATTC CCGCTCCGCA 
TTAAGATCTG AAATGTATCA GGATACTGAC CTTCCACTGC TGGATCAAGG AAGCGAGATT 
TTTAAGACTC TTCACTACCT CAGCATCCTG ATCCACAGTA TCAAAAACCC GCTGGGAACG 
CAGGAGAATC CGGCCAGAAT GTGCAGGGAT CTGCTTGAAT GCGAGCACCG GTTGAATGAT 
GGCACTTATT GGATAGATCC TAACCTGGGC TGCTCCTCTG ACAACATTGA AGTGACCTGT 
AGCTTCACCA GTGGAGGTCA AACCTGCCTT AAGCCAGTCA CTGCTTCCAA GCTGGAGATC 
GGAGTCAGTC TGATCCAGAT GAACTTCATC CACCTCCTGA GCTCTGAGGC TGTGCAGATC 
ATCACCGTTC ACTGTCTGAA CGTCTCCGTT TGGGCATCGG AAGACTCCAA AACACCTTCC 
TCCAGCATGG TATACTTTAA AGCCTGGGAT GGACAGATAA TCGAGGCTGG AGGATTCATC 
GAGCCTGATT TGCTGAAGGA TGAGTGCTGG ATCACAGATG GTAGATGGCA TCAAACACAG 
TTCATTTTCC GGACGCAGGA CCCAAACTTA CTACCCATTG TGGAGATCTA CAACTTGCCC 
AGCACTAAAC CTGGCTCACA TTACCACCTG GAGGTGGGCC CTGTGTGCTT TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Fishing for collagen function: About development, regeneration and disease.
Seminars in cell & developmental biology89100-108(2019 05)
Bretaud S,Nauroy P,Malbouyres M,Ruggiero F

Collagen duplicate genes of bone and cartilage participate during regeneration of zebrafish fin skeleton.
Gene expression patterns : GEP19(1-2)60-9(2015 Sep-Nov)
Duran I,Csukasi F,Taylor SP,Krakow D,Becerra J,Bombarely A,Marí-Beffa M

Critical early roles for col27a1a and col27a1b in zebrafish notochord morphogenesis, vertebral mineralization and post-embryonic axial growth.
PloS one4(12)e8481(2009 Dec)
Christiansen HE,Lang MR,Pace JM,Parichy DM

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

COL27A1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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