gbe1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gbe1b
Entrez Gene ID	559212
Full Name	glucan (1,4-alpha-), branching enzyme 1b
Synonyms	gbe1,si:ch211-247m23.1
General protein information	Preferred Names glucan (1,4-alpha-), branching enzyme 1b Names 1,4-alpha-glucan-branching enzyme glucan (1,4-alpha-), branching enzyme 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009291498.3	XP_009289773.1	1,4-alpha-glucan-branching enzyme isoform X1
XM_009291498.2	XP_009289773.1	1,4-alpha-glucan-branching enzyme isoform X1
XM_009291499.1	XP_009289774.1	1,4-alpha-glucan-branching enzyme isoform X2
XM_009291499.2	XP_009289774.1	1,4-alpha-glucan-branching enzyme isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	GBE1	XP_425536.4
Danio rerio (zebrafish)	si:ch211-213e17.1	XP_002663606.3
Canis lupus familiaris (dog)	LOC478380	XP_535555.3
Rattus norvegicus (Norway rat)	Gbe1	NP_001093972.1
Arabidopsis thaliana (thale cress)	SBE2.1	NP_181180.1
Xenopus tropicalis (tropical clawed frog)	gbe1	XP_004912135.1
Oryza sativa (rice)	Os02g0528200	NP_001047009.1
Homo sapiens (human)	GBE1	NP_000149.3
Magnaporthe oryzae (rice blast fungus)	MGG_03186	XP_003716803.1
Kluyveromyces lactis	KLLA0A11176g	XP_451486.1
Neurospora crassa	NCU05429	XP_963252.2
Eremothecium gossypii	AGOS_AEL044W	NP_984817.1
Bos taurus (cattle)	GBE1	NP_001116201.1
Drosophila melanogaster (fruit fly)	CG33138	NP_001260941.1
Arabidopsis thaliana (thale cress)	SBE2.2	NP_195985.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010428	XP_311519.3
Pan troglodytes (chimpanzee)	GBE1	XP_516593.2
Macaca mulatta (Rhesus monkey)	GBE1	XP_001118968.2
Mus musculus (house mouse)	Gbe1	NP_083079.1
Danio rerio (zebrafish)	LOC559212	XP_687620.6
Saccharomyces cerevisiae (baker's yeast)	GLC3	NP_010905.1
Caenorhabditis elegans (roundworm)	CELE_T04A8.7	NP_497961.1

Gene Ontology

The following gbe1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gbe1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa43008	XM_009291499.2 Latest version!	Danio rerio glucan (1,4-alpha-), branching enzyme 1b (gbe1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa20231	XM_009291498.3 Latest version!	Danio rerio glucan (1,4-alpha-), branching enzyme 1b (gbe1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
ODa20231	XM_009291498.2	Danio rerio glucan (1,4-alpha-), branching enzyme 1b (gbe1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa43008	XM_009291499.1	Danio rerio glucan (1,4-alpha-), branching enzyme 1b (gbe1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gbe1b ( XM_009291499.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289774.1 Danio rerio glucan (1,4-alpha-), branching enzyme 1b (gbe1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa43008
Clone ID Related Accession (Same CDS sequence)	XM_009291499.1 , XM_009291499.2
Accession Version	XM_009291499.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	1,4-alpha-glucan-branching enzyme isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGT ACAAGTTTGA TGGATTCAGG TTCGACGGAG TCACATCCAT GCTCTACCAT 
CATCACGGCA TCGGATCTGG ATTTTCTGGG GATTATAACG AGTACTTTGG CCTGCAGGTG 
GATGAGGACT CTCTGGTCTA CCTGATGTTG GCGAACCATA TTTTACACAC TCTCTATGAA 
GACTGCATCA CTATCGCTGA GGATGTGTCC GGGATGCCTA CACTTTGCTG TCCAGTCCAA 
CAAGGAGGTC TGGGTTTTGA CTACCGCCTG GCCATGGCCA TTCCAGACAA ATGGATTCAA 
ATCCTCAAGG AATTCAAGGA CGAAGACTGG AACATGGGAA ACATTGTGTT TACGATGACC 
AACAGGCGAT ATGGAGAGAA ATGCATTGCT TACGCCGAGA GCCATGACCA GGCACTGGTG 
GGAGACAAAA CGCTGGCCTT CTGGCTGATG GACAAAGAGA TGTACACCAG CATGAGCGCT 
TTGATTCCCA TGAACTCCAT TATTGATCGG GGCATCCAGC TTCATAAGAT GATCCGACTG 
CTTACCCACA GCCTGGGAGG AGAGGGATAC CTCAACTTCA TGGGCAATGA GTTTGGCCAT 
CCAGAGTGGC TGGATTTTCC ACGAATCGGC AATAATGAGA GTTATCATTA TGCCCGCCGG 
CAGTATAACC TGGTGGACAT GGATCATCTG CGCTACCGGC AGCTGTACGC TTTTGACCGA 
GACATGAACC GCACGGAGGA CAACTACGGC TGGCTGGCTG CTCCACCGGC ATACGTCAGT 
GTCAAGCATG AGGGTGACAA GGTGATCGTA TTCGAGAGAG CAAACCTCAT CTTCATCTTC 
AACTTCCACC CTTTCAACAG CTACAGCGAC TACAGGGTGG CCGTGGGGCC GGCGGGGAAA 
TATAAAATCA AGCTGGACTC TGATGAGATC CAATATGGAG GCCATGGCAG ACTGGATCAC 
AATACTGAGT TCTTCACTGA GGCAATGGGA CTTAACAATC GGCCAAACTC TATGATGGTT 
TACATTCCCT GCAGAACCGC TATTGTTCTG GCCAACGAGG AAATCGATTA CTGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289774.1
CDS	537..1616
Translation	MEEYKFDGFRFDGVTSMLYHHHGIGSGFSGDYNEYFGLQVDEDSLVYLMLANHILHTLYEDCITIAEDVSGMPTLCCPVQQGGLGFDYRLAMAIPDKWIQILKEFKDEDWNMGNIVFTMTNRRYGEKCIAYAESHDQALVGDKTLAFWLMDKEMYTSMSALIPMNSIIDRGIQLHKMIRLLTHSLGGEGYLNFMGNEFGHPEWLDFPRIGNNESYHYARRQYNLVDMDHLRYRQLYAFDRDMNRTEDNYGWLAAPPAYVSVKHEGDKVIVFERANLIFIFNFHPFNSYSDYRVAVGPAGKYKIKLDSDEIQYGGHGRLDHNTEFFTEAMGLNNRPNSMMVYIPCRTAIVLANEEIDYCY

Target ORF information:

RefSeq Version	XM_009291499.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glucan (1,4-alpha-), branching enzyme 1b (gbe1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291499.1

ORF Insert Sequence:

ATGGAGGAGT ACAAGTTTGA TGGATTCAGG TTCGACGGAG TCACATCCAT GCTCTACCAT 
CATCACGGCA TCGGATCTGG ATTTTCTGGG GATTATAACG AGTACTTTGG CCTGCAGGTG 
GATGAGGACT CTCTGGTCTA CCTGATGTTG GCGAACCATA TTTTACACAC TCTCTATGAA 
GACTGCATCA CTATCGCTGA GGATGTGTCC GGGATGCCTA CACTTTGCTG TCCAGTCCAA 
CAAGGAGGTC TGGGTTTTGA CTACCGCCTG GCCATGGCCA TTCCAGACAA ATGGATTCAA 
ATCCTCAAGG AATTCAAGGA CGAAGACTGG AACATGGGAA ACATTGTGTT TACGATGACC 
AACAGGCGAT ATGGAGAGAA ATGCATTGCT TACGCCGAGA GCCATGACCA GGCACTGGTG 
GGAGACAAAA CGCTGGCCTT CTGGCTGATG GACAAAGAGA TGTACACCAG CATGAGCGCT 
TTGATTCCCA TGAACTCCAT TATTGATCGG GGCATCCAGC TTCATAAGAT GATCCGACTG 
CTTACCCACA GCCTGGGAGG AGAGGGATAC CTCAACTTCA TGGGCAATGA GTTTGGCCAT 
CCAGAGTGGC TGGATTTTCC ACGAATCGGC AATAATGAGA GTTATCATTA TGCCCGCCGG 
CAGTATAACC TGGTGGACAT GGATCATCTG CGCTACCGGC AGCTGTACGC TTTTGACCGA 
GACATGAACC GCACGGAGGA CAACTACGGC TGGCTGGCTG CTCCACCGGC ATACGTCAGT 
GTCAAGCATG AGGGTGACAA GGTGATCGTA TTCGAGAGAG CAAACCTCAT CTTCATCTTC 
AACTTCCACC CTTTCAACAG CTACAGCGAC TACAGGGTGG CCGTGGGGCC GGCGGGGAAA 
TATAAAATCA AGCTGGACTC TGATGAGATC CAATATGGAG GCCATGGCAG ACTGGATCAC 
AATACTGAGT TCTTCACTGA GGCAATGGGA CTTAACAATC GGCCAAACTC TATGATGGTT 
TACATTCCCT GCAGAACCGC TATTGTTCTG GCCAACGAGG AAATCGATTA CTGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gbe1b ( XM_009291499.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289774.1 Danio rerio glucan (1,4-alpha-), branching enzyme 1b (gbe1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa43008
Clone ID Related Accession (Same CDS sequence)	XM_009291499.1 , XM_009291499.2
Accession Version	XM_009291499.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	1,4-alpha-glucan-branching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291499.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGT ACAAGTTTGA TGGATTCAGG TTCGACGGAG TCACATCCAT GCTCTACCAT 
CATCACGGCA TCGGATCTGG ATTTTCTGGG GATTATAACG AGTACTTTGG CCTGCAGGTG 
GATGAGGACT CTCTGGTCTA CCTGATGTTG GCGAACCATA TTTTACACAC TCTCTATGAA 
GACTGCATCA CTATCGCTGA GGATGTGTCC GGGATGCCTA CACTTTGCTG TCCAGTCCAA 
CAAGGAGGTC TGGGTTTTGA CTACCGCCTG GCCATGGCCA TTCCAGACAA ATGGATTCAA 
ATCCTCAAGG AATTCAAGGA CGAAGACTGG AACATGGGAA ACATTGTGTT TACGATGACC 
AACAGGCGAT ATGGAGAGAA ATGCATTGCT TACGCCGAGA GCCATGACCA GGCACTGGTG 
GGAGACAAAA CGCTGGCCTT CTGGCTGATG GACAAAGAGA TGTACACCAG CATGAGCGCT 
TTGATTCCCA TGAACTCCAT TATTGATCGG GGCATCCAGC TTCATAAGAT GATCCGACTG 
CTTACCCACA GCCTGGGAGG AGAGGGATAC CTCAACTTCA TGGGCAATGA GTTTGGCCAT 
CCAGAGTGGC TGGATTTTCC ACGAATCGGC AATAATGAGA GTTATCATTA TGCCCGCCGG 
CAGTATAACC TGGTGGACAT GGATCATCTG CGCTACCGGC AGCTGTACGC TTTTGACCGA 
GACATGAACC GCACGGAGGA CAACTACGGC TGGCTGGCTG CTCCACCGGC ATACGTCAGT 
GTCAAGCATG AGGGTGACAA GGTGATCGTA TTCGAGAGAG CAAACCTCAT CTTCATCTTC 
AACTTCCACC CTTTCAACAG CTACAGCGAC TACAGGGTGG CCGTGGGGCC GGCGGGGAAA 
TATAAAATCA AGCTGGACTC TGATGAGATC CAATATGGAG GCCATGGCAG ACTGGATCAC 
AATACTGAGT TCTTCACTGA GGCAATGGGA CTTAACAATC GGCCAAACTC TATGATGGTT 
TACATTCCCT GCAGAACCGC TATTGTTCTG GCCAACGAGG AAATCGATTA CTGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289774.1
CDS	533..1612
Translation	MEEYKFDGFRFDGVTSMLYHHHGIGSGFSGDYNEYFGLQVDEDSLVYLMLANHILHTLYEDCITIAEDVSGMPTLCCPVQQGGLGFDYRLAMAIPDKWIQILKEFKDEDWNMGNIVFTMTNRRYGEKCIAYAESHDQALVGDKTLAFWLMDKEMYTSMSALIPMNSIIDRGIQLHKMIRLLTHSLGGEGYLNFMGNEFGHPEWLDFPRIGNNESYHYARRQYNLVDMDHLRYRQLYAFDRDMNRTEDNYGWLAAPPAYVSVKHEGDKVIVFERANLIFIFNFHPFNSYSDYRVAVGPAGKYKIKLDSDEIQYGGHGRLDHNTEFFTEAMGLNNRPNSMMVYIPCRTAIVLANEEIDYCY

Target ORF information:

RefSeq Version	XM_009291499.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glucan (1,4-alpha-), branching enzyme 1b (gbe1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291499.2

ORF Insert Sequence:

ATGGAGGAGT ACAAGTTTGA TGGATTCAGG TTCGACGGAG TCACATCCAT GCTCTACCAT 
CATCACGGCA TCGGATCTGG ATTTTCTGGG GATTATAACG AGTACTTTGG CCTGCAGGTG 
GATGAGGACT CTCTGGTCTA CCTGATGTTG GCGAACCATA TTTTACACAC TCTCTATGAA 
GACTGCATCA CTATCGCTGA GGATGTGTCC GGGATGCCTA CACTTTGCTG TCCAGTCCAA 
CAAGGAGGTC TGGGTTTTGA CTACCGCCTG GCCATGGCCA TTCCAGACAA ATGGATTCAA 
ATCCTCAAGG AATTCAAGGA CGAAGACTGG AACATGGGAA ACATTGTGTT TACGATGACC 
AACAGGCGAT ATGGAGAGAA ATGCATTGCT TACGCCGAGA GCCATGACCA GGCACTGGTG 
GGAGACAAAA CGCTGGCCTT CTGGCTGATG GACAAAGAGA TGTACACCAG CATGAGCGCT 
TTGATTCCCA TGAACTCCAT TATTGATCGG GGCATCCAGC TTCATAAGAT GATCCGACTG 
CTTACCCACA GCCTGGGAGG AGAGGGATAC CTCAACTTCA TGGGCAATGA GTTTGGCCAT 
CCAGAGTGGC TGGATTTTCC ACGAATCGGC AATAATGAGA GTTATCATTA TGCCCGCCGG 
CAGTATAACC TGGTGGACAT GGATCATCTG CGCTACCGGC AGCTGTACGC TTTTGACCGA 
GACATGAACC GCACGGAGGA CAACTACGGC TGGCTGGCTG CTCCACCGGC ATACGTCAGT 
GTCAAGCATG AGGGTGACAA GGTGATCGTA TTCGAGAGAG CAAACCTCAT CTTCATCTTC 
AACTTCCACC CTTTCAACAG CTACAGCGAC TACAGGGTGG CCGTGGGGCC GGCGGGGAAA 
TATAAAATCA AGCTGGACTC TGATGAGATC CAATATGGAG GCCATGGCAG ACTGGATCAC 
AATACTGAGT TCTTCACTGA GGCAATGGGA CTTAACAATC GGCCAAACTC TATGATGGTT 
TACATTCCCT GCAGAACCGC TATTGTTCTG GCCAACGAGG AAATCGATTA CTGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gbe1b ( XM_009291498.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289773.1 Danio rerio glucan (1,4-alpha-), branching enzyme 1b (gbe1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20231
Clone ID Related Accession (Same CDS sequence)	XM_009291498.3 , XM_009291498.2
Accession Version	XM_009291498.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	1,4-alpha-glucan-branching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291498.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAT CGGAGAGTAA CATTTCAGTT CCTCACTTGA AATCTCTTCT GCAAATGGAT 
CCCTACCTCA AGCCCTTTGA AAAGGACTTC GAGAGGAGGT ATGGGCTGTT TGAGAAGCAG 
CTGGCTTTCT TGGAGGAGGC TGAAGGAGGC TTTGATCATT TCACTCGCAG TTATGAGAGT 
TTTGGCGTTC AGCGGCTGCA GGACAACAGC CTGGTCTTTA AGGAATGGGC TCCGGCTGCA 
GAGGCTCTGT TCCTCACTGG AGACTTCAAT GGCTGGGATA AGTTTTCCCA TCCCTACGCT 
AAAAAGGAGT TTGGGAAATG GGAGCTTCAC ATTCCTCCGA AAGAGGACAA GACACCTGCC 
GTCACTCATA ACTCCAAGCT GAAGGTGGTT GTTCACACCA GTGCGGGTGA GCGGCTGTAC 
CGAATCTCTC CTTGGGCAAA GTATGTGACC AGGCATGAGA AGTCCGTCAT CTATGATTGG 
GTGCACTGGG ACCCTCCTCA ACCTTACATT CACAAGCATC CCCGTCCACA GAAGCCCAGG 
AGCTTGCGGA TATACGAGTC CCATGTTGGC ATTGCTTCTC CAGAGGGCAA AATTGCGTCC 
TACAGCAACT TCACCCACAA TGTGCTGCCC CGCATCAAAG ATCTGGGTTA TAACAGCATT 
CAGCTGATGG CCATCATGGA GCATGCCTAC TATGCTAGCT TCGGTTACCA GGTCACGAGT 
TTTTTTGCAG CATCAAGCCG TTACGGGACC CCGGAGGAGC TGAAGGAGCT GATTGACGTG 
GCTCATTCTC TCGGCATCAT CGTCCTGCTT GATGTGGTGC ACAGCCACGC GTCCAAAAAC 
ACAGAGGACG GCCTGAACCT GTTTGACGGC TCCGACTCCT GCTTCTTCCA CAGCGGGCCC 
CGTGGAGAAC ACAGCCTGTG GGACAGCAGA CTCTTCAACT ACTCCAGCTG GGAGGTGTTG 
CGTTTTCTCC TGTCGAACTT GCGCTGGTGG ATGGAGGAGT ACAAGTTTGA TGGATTCAGG 
TTCGACGGAG TCACATCCAT GCTCTACCAT CATCACGGCA TCGGATCTGG ATTTTCTGGG 
GATTATAACG AGTACTTTGG CCTGCAGGTG GATGAGGACT CTCTGGTCTA CCTGATGTTG 
GCGAACCATA TTTTACACAC TCTCTATGAA GACTGCATCA CTATCGCTGA GGATGTGTCC 
GGGATGCCTA CACTTTGCTG TCCAGTCCAA CAAGGAGGTC TGGGTTTTGA CTACCGCCTG 
GCCATGGCCA TTCCAGACAA ATGGATTCAA ATCCTCAAGG AATTCAAGGA CGAAGACTGG 
AACATGGGAA ACATTGTGTT TACGATGACC AACAGGCGAT ATGGAGAGAA ATGCATTGCT 
TACGCCGAGA GCCATGACCA GGCACTGGTG GGAGACAAAA CGCTGGCCTT CTGGCTGATG 
GACAAAGAGA TGTACACCAG CATGAGCGCT TTGATTCCCA TGAACTCCAT TATTGATCGG 
GGCATCCAGC TTCATAAGAT GATCCGACTG CTTACCCACA GCCTGGGAGG AGAGGGATAC 
CTCAACTTCA TGGGCAATGA GTTTGGCCAT CCAGAGTGGC TGGATTTTCC ACGAATCGGC 
AATAATGAGA GTTATCATTA TGCCCGCCGG CAGTATAACC TGGTGGACAT GGATCATCTG 
CGCTACCGGC AGCTGTACGC TTTTGACCGA GACATGAACC GCACGGAGGA CAACTACGGC 
TGGCTGGCTG CTCCACCGGC ATACGTCAGT GTCAAGCATG AGGGTGACAA GGTGATCGTA 
TTCGAGAGAG CAAACCTCAT CTTCATCTTC AACTTCCACC CTTTCAACAG CTACAGCGAC 
TACAGGGTGG CCGTGGGGCC GGCGGGGAAA TATAAAATCA AGCTGGACTC TGATGAGATC 
CAATATGGAG GCCATGGCAG ACTGGATCAC AATACTGAGT TCTTCACTGA GGCAATGGGA 
CTTAACAATC GGCCAAACTC TATGATGGTT TACATTCCCT GCAGAACCGC TATTGTTCTG 
GCCAACGAGG AAATCGATTA CTGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289773.1
CDS	201..2270
Translation	MAESESNISVPHLKSLLQMDPYLKPFEKDFERRYGLFEKQLAFLEEAEGGFDHFTRSYESFGVQRLQDNSLVFKEWAPAAEALFLTGDFNGWDKFSHPYAKKEFGKWELHIPPKEDKTPAVTHNSKLKVVVHTSAGERLYRISPWAKYVTRHEKSVIYDWVHWDPPQPYIHKHPRPQKPRSLRIYESHVGIASPEGKIASYSNFTHNVLPRIKDLGYNSIQLMAIMEHAYYASFGYQVTSFFAASSRYGTPEELKELIDVAHSLGIIVLLDVVHSHASKNTEDGLNLFDGSDSCFFHSGPRGEHSLWDSRLFNYSSWEVLRFLLSNLRWWMEEYKFDGFRFDGVTSMLYHHHGIGSGFSGDYNEYFGLQVDEDSLVYLMLANHILHTLYEDCITIAEDVSGMPTLCCPVQQGGLGFDYRLAMAIPDKWIQILKEFKDEDWNMGNIVFTMTNRRYGEKCIAYAESHDQALVGDKTLAFWLMDKEMYTSMSALIPMNSIIDRGIQLHKMIRLLTHSLGGEGYLNFMGNEFGHPEWLDFPRIGNNESYHYARRQYNLVDMDHLRYRQLYAFDRDMNRTEDNYGWLAAPPAYVSVKHEGDKVIVFERANLIFIFNFHPFNSYSDYRVAVGPAGKYKIKLDSDEIQYGGHGRLDHNTEFFTEAMGLNNRPNSMMVYIPCRTAIVLANEEIDYCY

Target ORF information:

RefSeq Version	XM_009291498.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glucan (1,4-alpha-), branching enzyme 1b (gbe1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291498.3

ORF Insert Sequence:

ATGGCAGAAT CGGAGAGTAA CATTTCAGTT CCTCACTTGA AATCTCTTCT GCAAATGGAT 
CCCTACCTCA AGCCCTTTGA AAAGGACTTC GAGAGGAGGT ATGGGCTGTT TGAGAAGCAG 
CTGGCTTTCT TGGAGGAGGC TGAAGGAGGC TTTGATCATT TCACTCGCAG TTATGAGAGT 
TTTGGCGTTC AGCGGCTGCA GGACAACAGC CTGGTCTTTA AGGAATGGGC TCCGGCTGCA 
GAGGCTCTGT TCCTCACTGG AGACTTCAAT GGCTGGGATA AGTTTTCCCA TCCCTACGCT 
AAAAAGGAGT TTGGGAAATG GGAGCTTCAC ATTCCTCCGA AAGAGGACAA GACACCTGCC 
GTCACTCATA ACTCCAAGCT GAAGGTGGTT GTTCACACCA GTGCGGGTGA GCGGCTGTAC 
CGAATCTCTC CTTGGGCAAA GTATGTGACC AGGCATGAGA AGTCCGTCAT CTATGATTGG 
GTGCACTGGG ACCCTCCTCA ACCTTACATT CACAAGCATC CCCGTCCACA GAAGCCCAGG 
AGCTTGCGGA TATACGAGTC CCATGTTGGC ATTGCTTCTC CAGAGGGCAA AATTGCGTCC 
TACAGCAACT TCACCCACAA TGTGCTGCCC CGCATCAAAG ATCTGGGTTA TAACAGCATT 
CAGCTGATGG CCATCATGGA GCATGCCTAC TATGCTAGCT TCGGTTACCA GGTCACGAGT 
TTTTTTGCAG CATCAAGCCG TTACGGGACC CCGGAGGAGC TGAAGGAGCT GATTGACGTG 
GCTCATTCTC TCGGCATCAT CGTCCTGCTT GATGTGGTGC ACAGCCACGC GTCCAAAAAC 
ACAGAGGACG GCCTGAACCT GTTTGACGGC TCCGACTCCT GCTTCTTCCA CAGCGGGCCC 
CGTGGAGAAC ACAGCCTGTG GGACAGCAGA CTCTTCAACT ACTCCAGCTG GGAGGTGTTG 
CGTTTTCTCC TGTCGAACTT GCGCTGGTGG ATGGAGGAGT ACAAGTTTGA TGGATTCAGG 
TTCGACGGAG TCACATCCAT GCTCTACCAT CATCACGGCA TCGGATCTGG ATTTTCTGGG 
GATTATAACG AGTACTTTGG CCTGCAGGTG GATGAGGACT CTCTGGTCTA CCTGATGTTG 
GCGAACCATA TTTTACACAC TCTCTATGAA GACTGCATCA CTATCGCTGA GGATGTGTCC 
GGGATGCCTA CACTTTGCTG TCCAGTCCAA CAAGGAGGTC TGGGTTTTGA CTACCGCCTG 
GCCATGGCCA TTCCAGACAA ATGGATTCAA ATCCTCAAGG AATTCAAGGA CGAAGACTGG 
AACATGGGAA ACATTGTGTT TACGATGACC AACAGGCGAT ATGGAGAGAA ATGCATTGCT 
TACGCCGAGA GCCATGACCA GGCACTGGTG GGAGACAAAA CGCTGGCCTT CTGGCTGATG 
GACAAAGAGA TGTACACCAG CATGAGCGCT TTGATTCCCA TGAACTCCAT TATTGATCGG 
GGCATCCAGC TTCATAAGAT GATCCGACTG CTTACCCACA GCCTGGGAGG AGAGGGATAC 
CTCAACTTCA TGGGCAATGA GTTTGGCCAT CCAGAGTGGC TGGATTTTCC ACGAATCGGC 
AATAATGAGA GTTATCATTA TGCCCGCCGG CAGTATAACC TGGTGGACAT GGATCATCTG 
CGCTACCGGC AGCTGTACGC TTTTGACCGA GACATGAACC GCACGGAGGA CAACTACGGC 
TGGCTGGCTG CTCCACCGGC ATACGTCAGT GTCAAGCATG AGGGTGACAA GGTGATCGTA 
TTCGAGAGAG CAAACCTCAT CTTCATCTTC AACTTCCACC CTTTCAACAG CTACAGCGAC 
TACAGGGTGG CCGTGGGGCC GGCGGGGAAA TATAAAATCA AGCTGGACTC TGATGAGATC 
CAATATGGAG GCCATGGCAG ACTGGATCAC AATACTGAGT TCTTCACTGA GGCAATGGGA 
CTTAACAATC GGCCAAACTC TATGATGGTT TACATTCCCT GCAGAACCGC TATTGTTCTG 
GCCAACGAGG AAATCGATTA CTGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gbe1b ( XM_009291498.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289773.1 Danio rerio glucan (1,4-alpha-), branching enzyme 1b (gbe1b), transcript variant X1, mRNA.

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CloneID	ODa20231
Clone ID Related Accession (Same CDS sequence)	XM_009291498.3 , XM_009291498.2
Accession Version	XM_009291498.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	1,4-alpha-glucan-branching enzyme isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291498.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAT CGGAGAGTAA CATTTCAGTT CCTCACTTGA AATCTCTTCT GCAAATGGAT 
CCCTACCTCA AGCCCTTTGA AAAGGACTTC GAGAGGAGGT ATGGGCTGTT TGAGAAGCAG 
CTGGCTTTCT TGGAGGAGGC TGAAGGAGGC TTTGATCATT TCACTCGCAG TTATGAGAGT 
TTTGGCGTTC AGCGGCTGCA GGACAACAGC CTGGTCTTTA AGGAATGGGC TCCGGCTGCA 
GAGGCTCTGT TCCTCACTGG AGACTTCAAT GGCTGGGATA AGTTTTCCCA TCCCTACGCT 
AAAAAGGAGT TTGGGAAATG GGAGCTTCAC ATTCCTCCGA AAGAGGACAA GACACCTGCC 
GTCACTCATA ACTCCAAGCT GAAGGTGGTT GTTCACACCA GTGCGGGTGA GCGGCTGTAC 
CGAATCTCTC CTTGGGCAAA GTATGTGACC AGGCATGAGA AGTCCGTCAT CTATGATTGG 
GTGCACTGGG ACCCTCCTCA ACCTTACATT CACAAGCATC CCCGTCCACA GAAGCCCAGG 
AGCTTGCGGA TATACGAGTC CCATGTTGGC ATTGCTTCTC CAGAGGGCAA AATTGCGTCC 
TACAGCAACT TCACCCACAA TGTGCTGCCC CGCATCAAAG ATCTGGGTTA TAACAGCATT 
CAGCTGATGG CCATCATGGA GCATGCCTAC TATGCTAGCT TCGGTTACCA GGTCACGAGT 
TTTTTTGCAG CATCAAGCCG TTACGGGACC CCGGAGGAGC TGAAGGAGCT GATTGACGTG 
GCTCATTCTC TCGGCATCAT CGTCCTGCTT GATGTGGTGC ACAGCCACGC GTCCAAAAAC 
ACAGAGGACG GCCTGAACCT GTTTGACGGC TCCGACTCCT GCTTCTTCCA CAGCGGGCCC 
CGTGGAGAAC ACAGCCTGTG GGACAGCAGA CTCTTCAACT ACTCCAGCTG GGAGGTGTTG 
CGTTTTCTCC TGTCGAACTT GCGCTGGTGG ATGGAGGAGT ACAAGTTTGA TGGATTCAGG 
TTCGACGGAG TCACATCCAT GCTCTACCAT CATCACGGCA TCGGATCTGG ATTTTCTGGG 
GATTATAACG AGTACTTTGG CCTGCAGGTG GATGAGGACT CTCTGGTCTA CCTGATGTTG 
GCGAACCATA TTTTACACAC TCTCTATGAA GACTGCATCA CTATCGCTGA GGATGTGTCC 
GGGATGCCTA CACTTTGCTG TCCAGTCCAA CAAGGAGGTC TGGGTTTTGA CTACCGCCTG 
GCCATGGCCA TTCCAGACAA ATGGATTCAA ATCCTCAAGG AATTCAAGGA CGAAGACTGG 
AACATGGGAA ACATTGTGTT TACGATGACC AACAGGCGAT ATGGAGAGAA ATGCATTGCT 
TACGCCGAGA GCCATGACCA GGCACTGGTG GGAGACAAAA CGCTGGCCTT CTGGCTGATG 
GACAAAGAGA TGTACACCAG CATGAGCGCT TTGATTCCCA TGAACTCCAT TATTGATCGG 
GGCATCCAGC TTCATAAGAT GATCCGACTG CTTACCCACA GCCTGGGAGG AGAGGGATAC 
CTCAACTTCA TGGGCAATGA GTTTGGCCAT CCAGAGTGGC TGGATTTTCC ACGAATCGGC 
AATAATGAGA GTTATCATTA TGCCCGCCGG CAGTATAACC TGGTGGACAT GGATCATCTG 
CGCTACCGGC AGCTGTACGC TTTTGACCGA GACATGAACC GCACGGAGGA CAACTACGGC 
TGGCTGGCTG CTCCACCGGC ATACGTCAGT GTCAAGCATG AGGGTGACAA GGTGATCGTA 
TTCGAGAGAG CAAACCTCAT CTTCATCTTC AACTTCCACC CTTTCAACAG CTACAGCGAC 
TACAGGGTGG CCGTGGGGCC GGCGGGGAAA TATAAAATCA AGCTGGACTC TGATGAGATC 
CAATATGGAG GCCATGGCAG ACTGGATCAC AATACTGAGT TCTTCACTGA GGCAATGGGA 
CTTAACAATC GGCCAAACTC TATGATGGTT TACATTCCCT GCAGAACCGC TATTGTTCTG 
GCCAACGAGG AAATCGATTA CTGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289773.1
CDS	198..2267
Translation	MAESESNISVPHLKSLLQMDPYLKPFEKDFERRYGLFEKQLAFLEEAEGGFDHFTRSYESFGVQRLQDNSLVFKEWAPAAEALFLTGDFNGWDKFSHPYAKKEFGKWELHIPPKEDKTPAVTHNSKLKVVVHTSAGERLYRISPWAKYVTRHEKSVIYDWVHWDPPQPYIHKHPRPQKPRSLRIYESHVGIASPEGKIASYSNFTHNVLPRIKDLGYNSIQLMAIMEHAYYASFGYQVTSFFAASSRYGTPEELKELIDVAHSLGIIVLLDVVHSHASKNTEDGLNLFDGSDSCFFHSGPRGEHSLWDSRLFNYSSWEVLRFLLSNLRWWMEEYKFDGFRFDGVTSMLYHHHGIGSGFSGDYNEYFGLQVDEDSLVYLMLANHILHTLYEDCITIAEDVSGMPTLCCPVQQGGLGFDYRLAMAIPDKWIQILKEFKDEDWNMGNIVFTMTNRRYGEKCIAYAESHDQALVGDKTLAFWLMDKEMYTSMSALIPMNSIIDRGIQLHKMIRLLTHSLGGEGYLNFMGNEFGHPEWLDFPRIGNNESYHYARRQYNLVDMDHLRYRQLYAFDRDMNRTEDNYGWLAAPPAYVSVKHEGDKVIVFERANLIFIFNFHPFNSYSDYRVAVGPAGKYKIKLDSDEIQYGGHGRLDHNTEFFTEAMGLNNRPNSMMVYIPCRTAIVLANEEIDYCY

Target ORF information:

RefSeq Version	XM_009291498.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glucan (1,4-alpha-), branching enzyme 1b (gbe1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291498.2

ORF Insert Sequence:

ATGGCAGAAT CGGAGAGTAA CATTTCAGTT CCTCACTTGA AATCTCTTCT GCAAATGGAT 
CCCTACCTCA AGCCCTTTGA AAAGGACTTC GAGAGGAGGT ATGGGCTGTT TGAGAAGCAG 
CTGGCTTTCT TGGAGGAGGC TGAAGGAGGC TTTGATCATT TCACTCGCAG TTATGAGAGT 
TTTGGCGTTC AGCGGCTGCA GGACAACAGC CTGGTCTTTA AGGAATGGGC TCCGGCTGCA 
GAGGCTCTGT TCCTCACTGG AGACTTCAAT GGCTGGGATA AGTTTTCCCA TCCCTACGCT 
AAAAAGGAGT TTGGGAAATG GGAGCTTCAC ATTCCTCCGA AAGAGGACAA GACACCTGCC 
GTCACTCATA ACTCCAAGCT GAAGGTGGTT GTTCACACCA GTGCGGGTGA GCGGCTGTAC 
CGAATCTCTC CTTGGGCAAA GTATGTGACC AGGCATGAGA AGTCCGTCAT CTATGATTGG 
GTGCACTGGG ACCCTCCTCA ACCTTACATT CACAAGCATC CCCGTCCACA GAAGCCCAGG 
AGCTTGCGGA TATACGAGTC CCATGTTGGC ATTGCTTCTC CAGAGGGCAA AATTGCGTCC 
TACAGCAACT TCACCCACAA TGTGCTGCCC CGCATCAAAG ATCTGGGTTA TAACAGCATT 
CAGCTGATGG CCATCATGGA GCATGCCTAC TATGCTAGCT TCGGTTACCA GGTCACGAGT 
TTTTTTGCAG CATCAAGCCG TTACGGGACC CCGGAGGAGC TGAAGGAGCT GATTGACGTG 
GCTCATTCTC TCGGCATCAT CGTCCTGCTT GATGTGGTGC ACAGCCACGC GTCCAAAAAC 
ACAGAGGACG GCCTGAACCT GTTTGACGGC TCCGACTCCT GCTTCTTCCA CAGCGGGCCC 
CGTGGAGAAC ACAGCCTGTG GGACAGCAGA CTCTTCAACT ACTCCAGCTG GGAGGTGTTG 
CGTTTTCTCC TGTCGAACTT GCGCTGGTGG ATGGAGGAGT ACAAGTTTGA TGGATTCAGG 
TTCGACGGAG TCACATCCAT GCTCTACCAT CATCACGGCA TCGGATCTGG ATTTTCTGGG 
GATTATAACG AGTACTTTGG CCTGCAGGTG GATGAGGACT CTCTGGTCTA CCTGATGTTG 
GCGAACCATA TTTTACACAC TCTCTATGAA GACTGCATCA CTATCGCTGA GGATGTGTCC 
GGGATGCCTA CACTTTGCTG TCCAGTCCAA CAAGGAGGTC TGGGTTTTGA CTACCGCCTG 
GCCATGGCCA TTCCAGACAA ATGGATTCAA ATCCTCAAGG AATTCAAGGA CGAAGACTGG 
AACATGGGAA ACATTGTGTT TACGATGACC AACAGGCGAT ATGGAGAGAA ATGCATTGCT 
TACGCCGAGA GCCATGACCA GGCACTGGTG GGAGACAAAA CGCTGGCCTT CTGGCTGATG 
GACAAAGAGA TGTACACCAG CATGAGCGCT TTGATTCCCA TGAACTCCAT TATTGATCGG 
GGCATCCAGC TTCATAAGAT GATCCGACTG CTTACCCACA GCCTGGGAGG AGAGGGATAC 
CTCAACTTCA TGGGCAATGA GTTTGGCCAT CCAGAGTGGC TGGATTTTCC ACGAATCGGC 
AATAATGAGA GTTATCATTA TGCCCGCCGG CAGTATAACC TGGTGGACAT GGATCATCTG 
CGCTACCGGC AGCTGTACGC TTTTGACCGA GACATGAACC GCACGGAGGA CAACTACGGC 
TGGCTGGCTG CTCCACCGGC ATACGTCAGT GTCAAGCATG AGGGTGACAA GGTGATCGTA 
TTCGAGAGAG CAAACCTCAT CTTCATCTTC AACTTCCACC CTTTCAACAG CTACAGCGAC 
TACAGGGTGG CCGTGGGGCC GGCGGGGAAA TATAAAATCA AGCTGGACTC TGATGAGATC 
CAATATGGAG GCCATGGCAG ACTGGATCAC AATACTGAGT TCTTCACTGA GGCAATGGGA 
CTTAACAATC GGCCAAACTC TATGATGGTT TACATTCCCT GCAGAACCGC TATTGTTCTG 
GCCAACGAGG AAATCGATTA CTGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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gbe1b cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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